LOCUS       NM_001379672            9374 bp    mRNA    linear   PRI 21-SEP-2024
DEFINITION  Homo sapiens guanylate cyclase 1 soluble subunit alpha 1 (GUCY1A1),
            transcript variant 15, mRNA.
ACCESSION   NM_001379672
VERSION     NM_001379672.1
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 9374)
  AUTHORS   Guey,S., Herve,D., Kossorotoff,M., Ha,G., Aloui,C., Bergametti,F.,
            Arnould,M., Guenou,H., Hadjadj,J., Dubois Teklali,F., Riant,F.,
            Balligand,J.L., Uzan,G., Villoutreix,B.O. and Tournier-Lasserve,E.
  TITLE     Biallelic variants in NOS3 and GUCY1A3, the two major genes of the
            nitric oxide pathway, cause moyamoya cerebral angiopathy
  JOURNAL   Hum Genomics 17 (1), 24 (2023)
   PUBMED   36941667
  REMARK    GeneRIF: Biallelic variants in NOS3 and GUCY1A3, the two major
            genes of the nitric oxide pathway, cause moyamoya cerebral
            angiopathy.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9374)
  AUTHORS   Liu,R., Kang,Y. and Chen,L.
  TITLE     Activation mechanism of human soluble guanylate cyclase by
            stimulators and activators
  JOURNAL   Nat Commun 12 (1), 5492 (2021)
   PUBMED   34535643
  REMARK    GeneRIF: Activation mechanism of human soluble guanylate cyclase by
            stimulators and activators.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 9374)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   4  (bases 1 to 9374)
  AUTHORS   Sharina,I., Lezgyieva,K., Krutsenko,Y. and Martin,E.
  TITLE     Higher susceptibility to heme oxidation and lower protein stability
            of the rare alpha1C517Ybeta1 sGC variant associated with moyamoya
            syndrome
  JOURNAL   Biochem Pharmacol 186, 114459 (2021)
   PUBMED   33571505
  REMARK    GeneRIF: Higher susceptibility to heme oxidation and lower protein
            stability of the rare alpha1C517Ybeta1 sGC variant associated with
            moyamoya syndrome.
REFERENCE   5  (bases 1 to 9374)
  AUTHORS   Korkmaz,Y., Puladi,B., Galler,K., Kammerer,P.W., Schroder,A.,
            Golz,L., Sparwasser,T., Bloch,W., Friebe,A. and Deschner,J.
  TITLE     Inflammation in the Human Periodontium Induces Downregulation of
            the alpha1- and beta1-Subunits of the sGC in Cementoclasts
  JOURNAL   Int J Mol Sci 22 (2), 539 (2021)
   PUBMED   33430449
  REMARK    GeneRIF: Inflammation in the Human Periodontium Induces
            Downregulation of the alpha1- and beta1-Subunits of the sGC in
            Cementoclasts.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 9374)
  AUTHORS   Zhou,Y., Zheng,J.B., Gu,X., Li,W. and Saunders,G.F.
  TITLE     A novel Pax-6 binding site in rodent B1 repetitive elements:
            coevolution between developmental regulation and repeated elements?
  JOURNAL   Gene 245 (2), 319-328 (2000)
   PUBMED   10717483
REFERENCE   7  (bases 1 to 9374)
  AUTHORS   Zabel,U., Weeger,M., La,M. and Schmidt,H.H.
  TITLE     Human soluble guanylate cyclase: functional expression and revised
            isoenzyme family
  JOURNAL   Biochem J 335 (Pt 1) (Pt 1), 51-57 (1998)
   PUBMED   9742212
REFERENCE   8  (bases 1 to 9374)
  AUTHORS   Papapetropoulos,A., Cziraki,A., Rubin,J.W., Stone,C.D. and
            Catravas,J.D.
  TITLE     cGMP accumulation and gene expression of soluble guanylate cyclase
            in human vascular tissue
  JOURNAL   J Cell Physiol 167 (2), 213-221 (1996)
   PUBMED   8613461
REFERENCE   9  (bases 1 to 9374)
  AUTHORS   Giuili,G., Roechel,N., Scholl,U., Mattei,M.G. and Guellaen,G.
  TITLE     Colocalization of the genes coding for the alpha 3 and beta 3
            subunits of soluble guanylyl cyclase to human chromosome 4 at
            q31.3-q33
  JOURNAL   Hum Genet 91 (3), 257-260 (1993)
   PUBMED   8097486
REFERENCE   10 (bases 1 to 9374)
  AUTHORS   Giuili,G., Scholl,U., Bulle,F. and Guellaen,G.
  TITLE     Molecular cloning of the cDNAs coding for the two subunits of
            soluble guanylyl cyclase from human brain
  JOURNAL   FEBS Lett 304 (1), 83-88 (1992)
   PUBMED   1352257
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC104083.3.
            
            Summary: Soluble guanylate cyclases are heterodimeric proteins that
            catalyze the conversion of GTP to 3',5'-cyclic GMP and
            pyrophosphate. The protein encoded by this gene is an alpha subunit
            of this complex and it interacts with a beta subunit to form the
            guanylate cyclase enzyme, which is activated by nitric oxide.
            Several transcript variants encoding a few different isoforms have
            been found for this gene. [provided by RefSeq, Jan 2012].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038191.1648739.1,
                                           SRR1803616.228571.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1968189
                                           [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-361               AC104083.3         111837-112197
            362-728             AC104083.3         140931-141297
            729-790             AC104083.3         148107-148168
            791-849             AC104083.3         152411-152469
            850-1559            AC104083.3         154717-155426
            1560-2045           AC104083.3         157273-157758
            2046-2189           AC104083.3         161334-161477
            2190-2344           AC104083.3         166213-166367
            2345-9374           AC104083.3         174205-181234
FEATURES             Location/Qualifiers
     source          1..9374
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="4"
                     /map="4q32.1"
     gene            1..9374
                     /gene="GUCY1A1"
                     /gene_synonym="GC-S-alpha-1; GC-SA3; GCS-alpha-3; GUC1A3;
                     GUCA3; GUCSA3; GUCY1A3; MYMY6"
                     /note="guanylate cyclase 1 soluble subunit alpha 1"
                     /db_xref="GeneID:2982"
                     /db_xref="HGNC:HGNC:4685"
                     /db_xref="MIM:139396"
     exon            1..361
                     /gene="GUCY1A1"
                     /gene_synonym="GC-S-alpha-1; GC-SA3; GCS-alpha-3; GUC1A3;
                     GUCA3; GUCSA3; GUCY1A3; MYMY6"
                     /inference="alignment:Splign:2.1.0"
     exon            362..728
                     /gene="GUCY1A1"
                     /gene_synonym="GC-S-alpha-1; GC-SA3; GCS-alpha-3; GUC1A3;
                     GUCA3; GUCSA3; GUCY1A3; MYMY6"
                     /inference="alignment:Splign:2.1.0"
     CDS             474..2546
                     /gene="GUCY1A1"
                     /gene_synonym="GC-S-alpha-1; GC-SA3; GCS-alpha-3; GUC1A3;
                     GUCA3; GUCSA3; GUCY1A3; MYMY6"
                     /EC_number="4.6.1.2"
                     /note="isoform A is encoded by transcript variant 15;
                     soluble guanylate cyclase large subunit; guanylate cyclase
                     1, soluble, alpha 3; guanylate cyclase soluble subunit
                     alpha-1"
                     /codon_start=1
                     /product="guanylate cyclase soluble subunit alpha-1
                     isoform A"
                     /protein_id="NP_001366601.1"
                     /db_xref="CCDS:CCDS34085.1"
                     /db_xref="GeneID:2982"
                     /db_xref="HGNC:HGNC:4685"
                     /db_xref="MIM:139396"
                     /translation="MFCTKLKDLKITGECPFSLLAPGQVPNESSEEAAGSSESCKATV
                     PICQDIPEKNIQESLPQRKTSRSRVYLHTLAESICKLIFPEFERLNVALQRTLAKHKI
                     KESRKSLEREDFEKTIAEQAVAAGVPVEVIKESLGEEVFKICYEEDENILGVVGGTLK
                     DFLNSFSTLLKQSSHCQEAGKRGRLEDASILCLDKEDDFLHVYYFFPKRTTSLILPGI
                     IKAAAHVLYETEVEVSLMPPCFHNDCSEFVNQPYLLYSVHMKSTKPSLSPSKPQSSLV
                     IPTSLFCKTFPFHFMFDKDMTILQFGNGIRRLMNRRDFQGKPNFEEYFEILTPKINQT
                     FSGIMTMLNMQFVVRVRRWDNSVKKSSRVMDLKGQMIYIVESSAILFLGSPCVDRLED
                     FTGRGLYLSDIPIHNALRDVVLIGEQARAQDGLKKRLGKLKATLEQAHQALEEEKKKT
                     VDLLCSIFPCEVAQQLWQGQVVQAKKFSNVTMLFSDIVGFTAICSQCSPLQVITMLNA
                     LYTRFDQQCGELDVYKVETIGDAYCVAGGLHKESDTHAVQIALMALKMMELSDEVMSP
                     HGEPIKMRIGLHSGSVFAGVVGVKMPRYCLFGNNVTLANKFESCSVPRKINVSPTTYR
                     LLKDCPGFVFTPRSREELPPNFPSEIPGICHFLDAYQQGTNSKPCFQKKDVEDGNANF
                     LGKASGID"
     exon            729..790
                     /gene="GUCY1A1"
                     /gene_synonym="GC-S-alpha-1; GC-SA3; GCS-alpha-3; GUC1A3;
                     GUCA3; GUCSA3; GUCY1A3; MYMY6"
                     /inference="alignment:Splign:2.1.0"
     exon            791..849
                     /gene="GUCY1A1"
                     /gene_synonym="GC-S-alpha-1; GC-SA3; GCS-alpha-3; GUC1A3;
                     GUCA3; GUCSA3; GUCY1A3; MYMY6"
                     /inference="alignment:Splign:2.1.0"
     exon            850..1559
                     /gene="GUCY1A1"
                     /gene_synonym="GC-S-alpha-1; GC-SA3; GCS-alpha-3; GUC1A3;
                     GUCA3; GUCSA3; GUCY1A3; MYMY6"
                     /inference="alignment:Splign:2.1.0"
     exon            1560..2045
                     /gene="GUCY1A1"
                     /gene_synonym="GC-S-alpha-1; GC-SA3; GCS-alpha-3; GUC1A3;
                     GUCA3; GUCSA3; GUCY1A3; MYMY6"
                     /inference="alignment:Splign:2.1.0"
     exon            2046..2189
                     /gene="GUCY1A1"
                     /gene_synonym="GC-S-alpha-1; GC-SA3; GCS-alpha-3; GUC1A3;
                     GUCA3; GUCSA3; GUCY1A3; MYMY6"
                     /inference="alignment:Splign:2.1.0"
     exon            2190..2344
                     /gene="GUCY1A1"
                     /gene_synonym="GC-S-alpha-1; GC-SA3; GCS-alpha-3; GUC1A3;
                     GUCA3; GUCSA3; GUCY1A3; MYMY6"
                     /inference="alignment:Splign:2.1.0"
     exon            2345..9374
                     /gene="GUCY1A1"
                     /gene_synonym="GC-S-alpha-1; GC-SA3; GCS-alpha-3; GUC1A3;
                     GUCA3; GUCSA3; GUCY1A3; MYMY6"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3685..3690
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GUCY1A1"
                     /gene_synonym="GC-S-alpha-1; GC-SA3; GCS-alpha-3; GUC1A3;
                     GUCA3; GUCSA3; GUCY1A3; MYMY6"
                     /note="hexamer: ATTAAA"
     polyA_site      3704
                     /gene="GUCY1A1"
                     /gene_synonym="GC-S-alpha-1; GC-SA3; GCS-alpha-3; GUC1A3;
                     GUCA3; GUCSA3; GUCY1A3; MYMY6"
                     /note="major polyA site"
     regulatory      4641..4646
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GUCY1A1"
                     /gene_synonym="GC-S-alpha-1; GC-SA3; GCS-alpha-3; GUC1A3;
                     GUCA3; GUCSA3; GUCY1A3; MYMY6"
                     /note="hexamer: AATAAA"
     polyA_site      4662
                     /gene="GUCY1A1"
                     /gene_synonym="GC-S-alpha-1; GC-SA3; GCS-alpha-3; GUC1A3;
                     GUCA3; GUCSA3; GUCY1A3; MYMY6"
                     /note="major polyA site"
     regulatory      9349..9354
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GUCY1A1"
                     /gene_synonym="GC-S-alpha-1; GC-SA3; GCS-alpha-3; GUC1A3;
                     GUCA3; GUCSA3; GUCY1A3; MYMY6"
                     /note="hexamer: AATAAA"
     polyA_site      9374
                     /gene="GUCY1A1"
                     /gene_synonym="GC-S-alpha-1; GC-SA3; GCS-alpha-3; GUC1A3;
                     GUCA3; GUCSA3; GUCY1A3; MYMY6"
                     /note="major polyA site"
ORIGIN      
        1 agacgggggt gggcgccgag cagcgccgcg ccggctgctg agctcgcggg gccgggccgt
       61 gagaacgcgc ggggcgcgcc ggggccgctg gtctcccacg gccagggcgc ctgggcccca
      121 gagcccatca gccgcgtgca gcgctgcggc ctccggcgcg ccggcaggca agcgagcggg
      181 cgcagctggg ggatgcctgg accccggagg cagcagggct ggaggcggtg gcgggcttca
      241 gggcaacttc acccggccgg ctgcggcgtg ggtaagaggc gcgtcctcct ccacagcaaa
      301 agctgcgatc gctgggctgc ccagagaagg cagccggcta actgcccagc ggctccacga
      361 gacatcccag ttaccagtgt ccttgaattg atagtggctt ctgtttgtca gtctcatata
      421 agaactacag ctcatcagga ggagatcgca gcagggtaag agacaccaac accatgttct
      481 gcacgaagct caaggatctc aagatcacag gagagtgtcc tttctcctta ctggcaccag
      541 gtcaagttcc taacgagtct tcagaggagg cagcaggaag ctcagagagc tgcaaagcaa
      601 ccgtgcccat ctgtcaagac attcctgaga agaacataca agaaagtctt cctcaaagaa
      661 aaaccagtcg gagccgagtc tatcttcaca ctttggcaga gagtatttgc aaactgattt
      721 tcccagagtt tgaacggctg aatgttgcac ttcagagaac attggcaaag cacaaaataa
      781 aagaaagcag gaaatctttg gaaagagaag actttgaaaa aacaattgca gagcaagcag
      841 ttgcagcagg agttccagtg gaggttatca aagaatctct tggtgaagag gtttttaaaa
      901 tatgttacga ggaagatgaa aacatccttg gggtggttgg aggcaccctt aaagattttt
      961 taaacagctt cagtaccctt ctgaaacaga gcagccattg ccaagaagca ggaaaaaggg
     1021 gcaggcttga ggacgcctcc attctatgcc tggataagga ggatgatttt ctacatgttt
     1081 actacttctt ccctaagaga accacctccc tgattcttcc cggcatcata aaggcagctg
     1141 ctcacgtatt atatgaaacg gaagtggaag tgtcgttaat gcctccctgc ttccataatg
     1201 attgcagcga gtttgtgaat cagccctact tgttgtactc cgttcacatg aaaagcacca
     1261 agccatccct gtcccccagc aaaccccagt cctcgctggt gattcccaca tcgctattct
     1321 gcaagacatt tccattccat ttcatgtttg acaaagatat gacaattctg caatttggca
     1381 atggcatcag aaggctgatg aacaggagag actttcaagg aaagcctaat tttgaagaat
     1441 actttgaaat tctgactcca aaaatcaacc agacgtttag cgggatcatg actatgttga
     1501 atatgcagtt tgttgtacga gtgaggagat gggacaactc tgtgaaaaaa tcttcaaggg
     1561 ttatggacct caaaggccaa atgatctaca ttgttgaatc cagtgcaatc ttgtttttgg
     1621 ggtcaccctg tgtggacaga ttagaagatt ttacaggacg agggctctac ctctcagaca
     1681 tcccaattca caatgcactg agggatgtgg tcttaatagg ggaacaagcc cgagctcaag
     1741 atggcctgaa gaagaggctg gggaagctga aggctaccct tgagcaagcc caccaagccc
     1801 tggaggagga gaagaaaaag acagtagacc ttctgtgctc catatttccc tgtgaggttg
     1861 ctcagcagct gtggcaaggg caagttgtgc aagccaagaa gttcagtaat gtcaccatgc
     1921 tcttctcaga catcgttggg ttcactgcca tctgctccca gtgctcaccg ctgcaggtca
     1981 tcaccatgct caatgcactg tacactcgct tcgaccagca gtgtggagag ctggatgtct
     2041 acaaggtgga gaccattggc gatgcctatt gtgtagctgg gggattacac aaagagagtg
     2101 atactcatgc tgttcagata gcgctgatgg ccctgaagat gatggagctc tctgatgaag
     2161 ttatgtctcc ccatggagaa cctatcaaga tgcgaattgg actgcactct ggatcagttt
     2221 ttgctggcgt cgttggagtt aaaatgcccc gttactgtct ttttggaaac aatgtcactc
     2281 tggctaacaa atttgagtcc tgcagtgtac cacgaaaaat caatgtcagc ccaacaactt
     2341 acagattact caaagactgt cctggtttcg tgtttacccc tcgatcaagg gaggaacttc
     2401 caccaaactt ccctagtgaa atccccggaa tctgccattt tctggatgct taccaacaag
     2461 gaacaaactc aaaaccatgc ttccaaaaga aagatgtgga agatggcaat gccaattttt
     2521 taggcaaagc atcaggaata gattagcaac ctatatacct atttataagt ctttggggtt
     2581 tgactcattg aagatgtgta gagcctctga aagcacttta gggattgtag atggctaaca
     2641 agcagtatta aaatttcagg agccaagtca caatctttct cctgtttaac atgacaaaat
     2701 gtatgtactc acttcagtac ttcagctctt caagaaaaaa aaaaaaaacc ttaaaaagct
     2761 acttttgtgg gagtatttct attatataac cagcacttac tacctgtact caaaattcag
     2821 caccttgtac atatatcaga taattgtagt caattgtaca aactgatgga gtcacctgca
     2881 atctcatatc ctggtggaat gccatggtta ttaaagtgtg tttgtgatag tgtcgtcaaa
     2941 aaaaaggttt ttgaatagaa attacattct ttgacaccct cagttctttc aaactttcaa
     3001 ctctgcattt tattgcattt tctcatttaa ctgttagtat atgtttgaat ataggctgac
     3061 tttttttctc tattgcactt tcttcttttt ttctttttag aaaataagca attaggggtt
     3121 agatgcaata tgaatataaa atagttctgt aaataccaaa tattattttt agttctggaa
     3181 gttaaaaata ctctccatag taacgtgtgt tataataata aatatttctt ttgtttgact
     3241 tttttaaaat tctacaatgg taaaagctat tttaatccat gttagcaatt taattcctat
     3301 taattcattt tactatcata atagcaaagt attcagaata aaagaggttt atatcttcta
     3361 ttaaaatgca gtaatactat tcaaatctaa tttagctgga agcaacatgg gagtaaagta
     3421 atcatccagg ggacccacca taaaggacat tggtaaaaca tttacacacg taaacacacg
     3481 tgcaggcaca cttggaacaa tatggaccca cttcagcaga catcttctat tgccatgctg
     3541 agctgtttaa agttttaaga agaaaatcta agtatggaag tagaaaataa gacttgttct
     3601 tttggtggat atctcatttt tcttgagtat ttgtgctgaa cattttggtg tatgtctatg
     3661 aactaagaat caagtttttg tctcattaaa tatacatata tgaagtgttt ttattctact
     3721 tgaaatatct tgacttaaaa aaaacgactg ttagtattga tgaagcaaat gaggcagtga
     3781 ctacattatg gaaatgtcta agtatttttt ccggaacttc aactttttct ttttgtttta
     3841 cataaacatt taagcagcta ggaactttta gttttagtta ggtcagttga agttctctgg
     3901 tgaactaaaa aatctacatt ttggttgggg ataaagattt gattttaaaa acagcttgaa
     3961 tttaaagcaa gaaaaagatg aacgttatag ttttaaaatc aatattagca gagccataga
     4021 taggctgagc tcttatttgg ggttctgaga aataactcag tttaaaactt ctttaactgc
     4081 ctcccttagt acccaggcta gcccatggtg acatcaggga tattcattac tgattggcct
     4141 ctagtattcc aagggtacta ctatagatat tacaatgatg ctgtttacta agtgataacc
     4201 ccgtatctta caggactgac tttgactcaa taaaccactc agcctattat tggaaagtgc
     4261 cataggcctt gagaaagtat agttacatcc tcacaaatat atgaagttaa gtcactactt
     4321 acaaggaaaa aaaatgtaaa tacatttttc tttctggaac cgaactctta ttatggggaa
     4381 ataatagaaa taaagaaaat gtaaaagcat gaaatggaat gtagtcttga tcaaacattt
     4441 cagcagacct caatttgctt acaatctgta ctcaaaaagt ttataattca atcagaattc
     4501 aaaaagtatt ttgtgtgttt gtgatgtatt agcatataca caaaacaaat gaggggaatg
     4561 tatataatct tggtgaaaag ttgtatggga gttttttgta cgattcctgc aacttttcta
     4621 taaacgtggc attatatcta aataaaaaat taccaaaaaa catattttca cagtgaagtt
     4681 aaatcatctg tgataggttc cccagaccag cctacaaagg cccattaaat acagaataat
     4741 ttgtggatct tcaaagagga tgaacataca tttccatgac agagtccata cgaaattcac
     4801 aatttagatg ctaaaaaatt taaggaatat ctttttttct ctatcttatc tgctttgaag
     4861 catagcgatt agcgagaaac ttttcagatc cagaccactg atggtagcca gagtagagac
     4921 cctggagcat agattcttaa aataaataag gggcccaagt cagagctttg aggtattgaa
     4981 gatggaaaaa tagtggacaa acagcctgta atcaggttgg ggattacggg tactgagtta
     5041 tgttgccatt taactattct aaaaaataag actgggtctc aagactgctt ccctaacaga
     5101 ctgtttctcc tgaaaatgca ggagctattc tttctgttct ggttatatag tttccattca
     5161 tggcttcttg tcctgttgtt ttttgtgtcg agtacactat ataaattatc tccttataca
     5221 tatttctcag gcaagcacag agttatactg aacttttcta aagatgcttt gcatcaagaa
     5281 gcaaagggaa atacagaatt aaaatgtttc tttccatttt gctttgtttt tctatatcga
     5341 tctagacttt gtaggaaaat gcaaagcgta tatttaagaa aacctaaata agaatagatt
     5401 catttactca ttttcattta ttcattcatg aagaatttat tgaatgccta ctatgtgcca
     5461 ggaatattgc tatatttttg aaatttaagg ataaaatatg gcccaatcta tcaagaaagg
     5521 aaagtgaaag acaaatgtaa gacataatta caacattctg tgggagcaca aagtagggga
     5581 ctcagttctt agagaagtct tttggtctta gagttcatca agcagagaag gggaaggaca
     5641 tggtccagga aaataaatca gcacacaaag agatggggtc ttgagggtgc atagtacatt
     5701 ctggacagcc agttacctgg gatgagttgg gagggaggag aataaggaca aaagaccatc
     5761 tgggcaaaaa tcacgaaggg gtatgtgtgt catgtaaagg tgtgccatga tagttattca
     5821 tattgctatt gtaatattaa tatatagtaa ttaactacac atgacacagc tttacatgac
     5881 cttaagtagt tatcaacatt accataatag taatattaat aactacaata agagccatta
     5941 ttattcactt gaggcacttg ttcaaaatag attttacaaa agaaaaaaaa aaaagaagca
     6001 acatgagtaa aattagatcc attggctact gactccctct ccaacagttt taaagctact
     6061 agataagaga agcatcaagt cttaaaggaa gagaatggta tgatcagatg tgtgttttcc
     6121 aaagactatt agaaacagca tggttaagga tagggtggat atttacttgc acaggtgact
     6181 gaatgaacat ctgtttatac atcaagggaa aatgattcat tttatatcct ggatatttta
     6241 ccataagtat gagcataaag ctgggatgtc tggcattgga attttatgtc actctcaagg
     6301 ctaattagtc tttcagatcc tttggtaggg aggaaagaag agttgcttca gcattttgac
     6361 taacatttct tccaaatttc ttctccctga tatttccagc cacgccaagg caaagacttc
     6421 ccagctctgt ctcaggttag gttaaaagaa ggcataggat ggaagagggt ggtctgtggt
     6481 aggcctagca aatgaaagag atgaataaac aagagtgtct agtctaacta gtgcctgagg
     6541 aatagacatc agtcctctga gattacccag ctggaaaact gcccagatgg agctttacat
     6601 gcaaagctcc agtgaagtct agtgctgtat aatgctttaa atccacaatc aagcccttgt
     6661 tgatgcaaat gttagtcgcc caaagcagaa tgggttaacg aattactgca gtggttttag
     6721 gtgcatgggt ttgacccatg taaatcattt taataagtaa aattgaatct ttgatacata
     6781 tttgttgagg agcttgtcag atcactacct ttcactgaac agtaaaacat ggatcattta
     6841 tttcacctca ttttgcactt caatgcaatt tcactcttta ggtggttgta cttggacagt
     6901 taaatgaaga ctgtctttaa gggtgcaggg atggagaaca cagcatatat gagaggtggg
     6961 agggcagaga cgccttgcga ttcctgtgag gagggactta ccgtctcctc caaaacagaa
     7021 attcagtgct gcagagacgg tagcaaagtg cagttctctt gttaaaacac aggctaaaag
     7081 cagtgactgc ttaacttatt aagcagagaa aaccaaaatg tcattttcaa ccaaaattga
     7141 gtgttagagt ttatggtctc tgttatgata cttttcaaga cctgggaaat atttgtatcc
     7201 tttagtgaaa aggggaagaa tcaattactg aaagccataa gcttacagta acggagccaa
     7261 ttaatccttt gtcatctttg ttgagtttag ttttcttctg ttcttagcac gtcagctagt
     7321 agatctttga tgaactgttg ctcctcttta aatgtttctt ttattatatt gatgatgtgc
     7381 actcattcag tattcttatt ttaagctgca gtaatcactg tacatttgat tgccatctgt
     7441 acttcccttg aagcagcttt gcttattaca gccacacctg catctgtttt gactagccca
     7501 cataacctac acatttttca tccaaagcta tcccattacc ttatttatgt ccatttattt
     7561 tcatatatag tatctgccaa aatagtagca aaagttttat caatgtgtag atttcaatca
     7621 gaaaaaccta agcaaaagga aaaaaaagtg atattctaaa agccacactt cttttatcac
     7681 cagtgcagtt ttttccatca tcataaactt agttttatga aaatgttaat atcttggatt
     7741 taaacattgg ggtagaaatc aggacaaaat ttaaatgtca tatccaggac ttaatctcag
     7801 atattctggt agaagatatt tagataaggt tgtggctgcc tgtcaagttt tttaattttt
     7861 taaattatat aatgtcagaa aattaaatca caaatgagcc ttccagattg tgaggaaaat
     7921 tacttggctg aaaatgactt tgcataatgc ccaaatcttg aagtatctgc tcagatgttt
     7981 gttccatctg aaatgctgcc aaatccatct gtattgagga aagagctatg aggtagtttc
     8041 agagaaagtg aaaacaaaac aaagcaaaac aaaaatgcta ttacagaagt tggacagccc
     8101 aatgtgttaa gtattataca cctggaacaa gagcagcaaa actgaagctc tttggaaagt
     8161 tatggcaaca gtggtctggg aattctgggc ctttagaaat aatcattccc agtgatacag
     8221 ggatgtgcag catccagaca ctgcttaatc acagtgagct gccgtcttcc ctgaataagt
     8281 tgtatcttat aacattgaag tgaatgaaaa tgtattagta tttgagaatt tactattcct
     8341 tcacctcctt gcacatgtaa attaaagatg ttttggaaaa tatgagaact tgatgttcac
     8401 tgccctagag cactattctc caacctttta gattcctgca atctgttaat aaaatattgt
     8461 aggacaacaa ctctatagac tgatacatgc tgctctcaat ccatatacta catattagga
     8521 cagcttttct tttctaaatg agcatgaata taaatagaaa ttctagtatt ttcttcacct
     8581 accccagtga atcatcttta tattttactt gggaaactgc tacagttggc tagtgggtct
     8641 tcacttttat tgctttgaga aatggataaa agacatagac tatcacccct ccaaaaatgt
     8701 gcatatggcc ccatacaaga cacatgtact gccagagctt ggggaactcc ctagaatcta
     8761 ctcctagacc accctagtga agtaccatag accatattgt ctagatggga caaaataata
     8821 aattttgact aagaacttac tgtttagaga gaaaaaatag taaataatgt tcaaaatgag
     8881 aagacaataa ctgacaagtc tgtgattttt gggttagatg agaccacttt tgatttttga
     8941 ggaataaaag gagttagttt tttggtcctg atgtgtaaaa tgattcatgg ggtttcacat
     9001 acagacaagt agttttcttt tcagcaaata tttaacatat ctctcaatat atttttcacc
     9061 agtttttggt aaatatgcat tgctataggt ataccaagtt ttttttgtta tgtttagtaa
     9121 ctaatttcat tttttctgta tatgtaaata tagctattgg accctgcatt gaaaaccttc
     9181 ttagtactaa caacactcct tttattatta ccttcatagt tactttaaat gacatattta
     9241 tttcgagttt tacttctggc tgagttgcaa agtctatgag aatgttacct cctgtctcag
     9301 aagcttgtta gttattgtgc cacctaaata ttgtaataaa agtgtttaaa taaaaatctt
     9361 cttaaaattt aaaa
//
