LOCUS       NM_001130682            9205 bp    mRNA    linear   PRI 21-SEP-2024
DEFINITION  Homo sapiens guanylate cyclase 1 soluble subunit alpha 1 (GUCY1A1),
            transcript variant 2, mRNA.
ACCESSION   NM_001130682
VERSION     NM_001130682.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 9205)
  AUTHORS   Guey,S., Herve,D., Kossorotoff,M., Ha,G., Aloui,C., Bergametti,F.,
            Arnould,M., Guenou,H., Hadjadj,J., Dubois Teklali,F., Riant,F.,
            Balligand,J.L., Uzan,G., Villoutreix,B.O. and Tournier-Lasserve,E.
  TITLE     Biallelic variants in NOS3 and GUCY1A3, the two major genes of the
            nitric oxide pathway, cause moyamoya cerebral angiopathy
  JOURNAL   Hum Genomics 17 (1), 24 (2023)
   PUBMED   36941667
  REMARK    GeneRIF: Biallelic variants in NOS3 and GUCY1A3, the two major
            genes of the nitric oxide pathway, cause moyamoya cerebral
            angiopathy.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9205)
  AUTHORS   Liu,R., Kang,Y. and Chen,L.
  TITLE     Activation mechanism of human soluble guanylate cyclase by
            stimulators and activators
  JOURNAL   Nat Commun 12 (1), 5492 (2021)
   PUBMED   34535643
  REMARK    GeneRIF: Activation mechanism of human soluble guanylate cyclase by
            stimulators and activators.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 9205)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   4  (bases 1 to 9205)
  AUTHORS   Sharina,I., Lezgyieva,K., Krutsenko,Y. and Martin,E.
  TITLE     Higher susceptibility to heme oxidation and lower protein stability
            of the rare alpha1C517Ybeta1 sGC variant associated with moyamoya
            syndrome
  JOURNAL   Biochem Pharmacol 186, 114459 (2021)
   PUBMED   33571505
  REMARK    GeneRIF: Higher susceptibility to heme oxidation and lower protein
            stability of the rare alpha1C517Ybeta1 sGC variant associated with
            moyamoya syndrome.
REFERENCE   5  (bases 1 to 9205)
  AUTHORS   Korkmaz,Y., Puladi,B., Galler,K., Kammerer,P.W., Schroder,A.,
            Golz,L., Sparwasser,T., Bloch,W., Friebe,A. and Deschner,J.
  TITLE     Inflammation in the Human Periodontium Induces Downregulation of
            the alpha1- and beta1-Subunits of the sGC in Cementoclasts
  JOURNAL   Int J Mol Sci 22 (2), 539 (2021)
   PUBMED   33430449
  REMARK    GeneRIF: Inflammation in the Human Periodontium Induces
            Downregulation of the alpha1- and beta1-Subunits of the sGC in
            Cementoclasts.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 9205)
  AUTHORS   Zhou,Y., Zheng,J.B., Gu,X., Li,W. and Saunders,G.F.
  TITLE     A novel Pax-6 binding site in rodent B1 repetitive elements:
            coevolution between developmental regulation and repeated elements?
  JOURNAL   Gene 245 (2), 319-328 (2000)
   PUBMED   10717483
REFERENCE   7  (bases 1 to 9205)
  AUTHORS   Zabel,U., Weeger,M., La,M. and Schmidt,H.H.
  TITLE     Human soluble guanylate cyclase: functional expression and revised
            isoenzyme family
  JOURNAL   Biochem J 335 (Pt 1) (Pt 1), 51-57 (1998)
   PUBMED   9742212
REFERENCE   8  (bases 1 to 9205)
  AUTHORS   Papapetropoulos,A., Cziraki,A., Rubin,J.W., Stone,C.D. and
            Catravas,J.D.
  TITLE     cGMP accumulation and gene expression of soluble guanylate cyclase
            in human vascular tissue
  JOURNAL   J Cell Physiol 167 (2), 213-221 (1996)
   PUBMED   8613461
REFERENCE   9  (bases 1 to 9205)
  AUTHORS   Giuili,G., Roechel,N., Scholl,U., Mattei,M.G. and Guellaen,G.
  TITLE     Colocalization of the genes coding for the alpha 3 and beta 3
            subunits of soluble guanylyl cyclase to human chromosome 4 at
            q31.3-q33
  JOURNAL   Hum Genet 91 (3), 257-260 (1993)
   PUBMED   8097486
REFERENCE   10 (bases 1 to 9205)
  AUTHORS   Giuili,G., Scholl,U., Bulle,F. and Guellaen,G.
  TITLE     Molecular cloning of the cDNAs coding for the two subunits of
            soluble guanylyl cyclase from human brain
  JOURNAL   FEBS Lett 304 (1), 83-88 (1992)
   PUBMED   1352257
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA793192.1, Y15723.1,
            AC104083.3 and CD671731.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Jun 2, 2019 this sequence version replaced NM_001130682.2.
            
            Summary: Soluble guanylate cyclases are heterodimeric proteins that
            catalyze the conversion of GTP to 3',5'-cyclic GMP and
            pyrophosphate. The protein encoded by this gene is an alpha subunit
            of this complex and it interacts with a beta subunit to form the
            guanylate cyclase enzyme, which is activated by nitric oxide.
            Several transcript variants encoding a few different isoforms have
            been found for this gene. [provided by RefSeq, Jan 2012].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803617.185004.1,
                                           SRR1803611.279345.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000506455.6/ ENSP00000424361.1
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-278               DA793192.1         139-416
            279-1377            Y15723.1           498-1596
            1378-1378           AC104083.3         155414-155414
            1379-2534           Y15723.1           1598-2753
            2535-9091           AC104083.3         174564-181120
            9092-9205           CD671731.1         19-132              c
FEATURES             Location/Qualifiers
     source          1..9205
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="4"
                     /map="4q32.1"
     gene            1..9205
                     /gene="GUCY1A1"
                     /gene_synonym="GC-S-alpha-1; GC-SA3; GCS-alpha-3; GUC1A3;
                     GUCA3; GUCSA3; GUCY1A3; MYMY6"
                     /note="guanylate cyclase 1 soluble subunit alpha 1"
                     /db_xref="GeneID:2982"
                     /db_xref="HGNC:HGNC:4685"
                     /db_xref="MIM:139396"
     exon            1..118
                     /gene="GUCY1A1"
                     /gene_synonym="GC-S-alpha-1; GC-SA3; GCS-alpha-3; GUC1A3;
                     GUCA3; GUCSA3; GUCY1A3; MYMY6"
                     /inference="alignment:Splign:2.1.0"
     exon            119..192
                     /gene="GUCY1A1"
                     /gene_synonym="GC-S-alpha-1; GC-SA3; GCS-alpha-3; GUC1A3;
                     GUCA3; GUCSA3; GUCY1A3; MYMY6"
                     /inference="alignment:Splign:2.1.0"
     exon            193..559
                     /gene="GUCY1A1"
                     /gene_synonym="GC-S-alpha-1; GC-SA3; GCS-alpha-3; GUC1A3;
                     GUCA3; GUCSA3; GUCY1A3; MYMY6"
                     /inference="alignment:Splign:2.1.0"
     CDS             305..2377
                     /gene="GUCY1A1"
                     /gene_synonym="GC-S-alpha-1; GC-SA3; GCS-alpha-3; GUC1A3;
                     GUCA3; GUCSA3; GUCY1A3; MYMY6"
                     /EC_number="4.6.1.2"
                     /note="isoform A is encoded by transcript variant 2;
                     soluble guanylate cyclase large subunit; guanylate cyclase
                     1, soluble, alpha 3; guanylate cyclase soluble subunit
                     alpha-1"
                     /codon_start=1
                     /product="guanylate cyclase soluble subunit alpha-1
                     isoform A"
                     /protein_id="NP_001124154.1"
                     /db_xref="CCDS:CCDS34085.1"
                     /db_xref="GeneID:2982"
                     /db_xref="HGNC:HGNC:4685"
                     /db_xref="MIM:139396"
                     /translation="MFCTKLKDLKITGECPFSLLAPGQVPNESSEEAAGSSESCKATV
                     PICQDIPEKNIQESLPQRKTSRSRVYLHTLAESICKLIFPEFERLNVALQRTLAKHKI
                     KESRKSLEREDFEKTIAEQAVAAGVPVEVIKESLGEEVFKICYEEDENILGVVGGTLK
                     DFLNSFSTLLKQSSHCQEAGKRGRLEDASILCLDKEDDFLHVYYFFPKRTTSLILPGI
                     IKAAAHVLYETEVEVSLMPPCFHNDCSEFVNQPYLLYSVHMKSTKPSLSPSKPQSSLV
                     IPTSLFCKTFPFHFMFDKDMTILQFGNGIRRLMNRRDFQGKPNFEEYFEILTPKINQT
                     FSGIMTMLNMQFVVRVRRWDNSVKKSSRVMDLKGQMIYIVESSAILFLGSPCVDRLED
                     FTGRGLYLSDIPIHNALRDVVLIGEQARAQDGLKKRLGKLKATLEQAHQALEEEKKKT
                     VDLLCSIFPCEVAQQLWQGQVVQAKKFSNVTMLFSDIVGFTAICSQCSPLQVITMLNA
                     LYTRFDQQCGELDVYKVETIGDAYCVAGGLHKESDTHAVQIALMALKMMELSDEVMSP
                     HGEPIKMRIGLHSGSVFAGVVGVKMPRYCLFGNNVTLANKFESCSVPRKINVSPTTYR
                     LLKDCPGFVFTPRSREELPPNFPSEIPGICHFLDAYQQGTNSKPCFQKKDVEDGNANF
                     LGKASGID"
     exon            560..621
                     /gene="GUCY1A1"
                     /gene_synonym="GC-S-alpha-1; GC-SA3; GCS-alpha-3; GUC1A3;
                     GUCA3; GUCSA3; GUCY1A3; MYMY6"
                     /inference="alignment:Splign:2.1.0"
     exon            622..680
                     /gene="GUCY1A1"
                     /gene_synonym="GC-S-alpha-1; GC-SA3; GCS-alpha-3; GUC1A3;
                     GUCA3; GUCSA3; GUCY1A3; MYMY6"
                     /inference="alignment:Splign:2.1.0"
     exon            681..1390
                     /gene="GUCY1A1"
                     /gene_synonym="GC-S-alpha-1; GC-SA3; GCS-alpha-3; GUC1A3;
                     GUCA3; GUCSA3; GUCY1A3; MYMY6"
                     /inference="alignment:Splign:2.1.0"
     exon            1391..1876
                     /gene="GUCY1A1"
                     /gene_synonym="GC-S-alpha-1; GC-SA3; GCS-alpha-3; GUC1A3;
                     GUCA3; GUCSA3; GUCY1A3; MYMY6"
                     /inference="alignment:Splign:2.1.0"
     exon            1877..2020
                     /gene="GUCY1A1"
                     /gene_synonym="GC-S-alpha-1; GC-SA3; GCS-alpha-3; GUC1A3;
                     GUCA3; GUCSA3; GUCY1A3; MYMY6"
                     /inference="alignment:Splign:2.1.0"
     exon            2021..2175
                     /gene="GUCY1A1"
                     /gene_synonym="GC-S-alpha-1; GC-SA3; GCS-alpha-3; GUC1A3;
                     GUCA3; GUCSA3; GUCY1A3; MYMY6"
                     /inference="alignment:Splign:2.1.0"
     exon            2176..9205
                     /gene="GUCY1A1"
                     /gene_synonym="GC-S-alpha-1; GC-SA3; GCS-alpha-3; GUC1A3;
                     GUCA3; GUCSA3; GUCY1A3; MYMY6"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3516..3521
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GUCY1A1"
                     /gene_synonym="GC-S-alpha-1; GC-SA3; GCS-alpha-3; GUC1A3;
                     GUCA3; GUCSA3; GUCY1A3; MYMY6"
                     /note="hexamer: ATTAAA"
     polyA_site      3535
                     /gene="GUCY1A1"
                     /gene_synonym="GC-S-alpha-1; GC-SA3; GCS-alpha-3; GUC1A3;
                     GUCA3; GUCSA3; GUCY1A3; MYMY6"
                     /note="major polyA site"
     regulatory      4472..4477
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GUCY1A1"
                     /gene_synonym="GC-S-alpha-1; GC-SA3; GCS-alpha-3; GUC1A3;
                     GUCA3; GUCSA3; GUCY1A3; MYMY6"
                     /note="hexamer: AATAAA"
     polyA_site      4493
                     /gene="GUCY1A1"
                     /gene_synonym="GC-S-alpha-1; GC-SA3; GCS-alpha-3; GUC1A3;
                     GUCA3; GUCSA3; GUCY1A3; MYMY6"
                     /note="major polyA site"
     regulatory      9180..9185
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GUCY1A1"
                     /gene_synonym="GC-S-alpha-1; GC-SA3; GCS-alpha-3; GUC1A3;
                     GUCA3; GUCSA3; GUCY1A3; MYMY6"
                     /note="hexamer: AATAAA"
     polyA_site      9205
                     /gene="GUCY1A1"
                     /gene_synonym="GC-S-alpha-1; GC-SA3; GCS-alpha-3; GUC1A3;
                     GUCA3; GUCSA3; GUCY1A3; MYMY6"
                     /note="major polyA site"
ORIGIN      
        1 acacttttcc tgcgctagag cagcgagcag cctggaacag acccaggcgg aggacacctg
       61 tgggggaggg agcgcctgga ggagcttaga gaccccagcc gggcgtgatc tcaccatgtg
      121 cggatttgcg aggcgcgccc tggagctgct agagatccgg aagcacagcc ccgaggtgtg
      181 cgaagccacc aaacatccca gttaccagtg tccttgaatt gatagtggct tctgtttgtc
      241 agtctcatat aagaactaca gctcatcagg aggagatcgc agcagggtaa gagacaccaa
      301 caccatgttc tgcacgaagc tcaaggatct caagatcaca ggagagtgtc ctttctcctt
      361 actggcacca ggtcaagttc ctaacgagtc ttcagaggag gcagcaggaa gctcagagag
      421 ctgcaaagca accgtgccca tctgtcaaga cattcctgag aagaacatac aagaaagtct
      481 tcctcaaaga aaaaccagtc ggagccgagt ctatcttcac actttggcag agagtatttg
      541 caaactgatt ttcccagagt ttgaacggct gaatgttgca cttcagagaa cattggcaaa
      601 gcacaaaata aaagaaagca ggaaatcttt ggaaagagaa gactttgaaa aaacaattgc
      661 agagcaagca gttgcagcag gagttccagt ggaggttatc aaagaatctc ttggtgaaga
      721 ggtttttaaa atatgttacg aggaagatga aaacatcctt ggggtggttg gaggcaccct
      781 taaagatttt ttaaacagct tcagtaccct tctgaaacag agcagccatt gccaagaagc
      841 aggaaaaagg ggcaggcttg aggacgcctc cattctatgc ctggataagg aggatgattt
      901 tctacatgtt tactacttct tccctaagag aaccacctcc ctgattcttc ccggcatcat
      961 aaaggcagct gctcacgtat tatatgaaac ggaagtggaa gtgtcgttaa tgcctccctg
     1021 cttccataat gattgcagcg agtttgtgaa tcagccctac ttgttgtact ccgttcacat
     1081 gaaaagcacc aagccatccc tgtcccccag caaaccccag tcctcgctgg tgattcccac
     1141 atcgctattc tgcaagacat ttccattcca tttcatgttt gacaaagata tgacaattct
     1201 gcaatttggc aatggcatca gaaggctgat gaacaggaga gactttcaag gaaagcctaa
     1261 ttttgaagaa tactttgaaa ttctgactcc aaaaatcaac cagacgttta gcgggatcat
     1321 gactatgttg aatatgcagt ttgttgtacg agtgaggaga tgggacaact ctgtgaaaaa
     1381 atcttcaagg gttatggacc tcaaaggcca aatgatctac attgttgaat ccagtgcaat
     1441 cttgtttttg gggtcaccct gtgtggacag attagaagat tttacaggac gagggctcta
     1501 cctctcagac atcccaattc acaatgcact gagggatgtg gtcttaatag gggaacaagc
     1561 ccgagctcaa gatggcctga agaagaggct ggggaagctg aaggctaccc ttgagcaagc
     1621 ccaccaagcc ctggaggagg agaagaaaaa gacagtagac cttctgtgct ccatatttcc
     1681 ctgtgaggtt gctcagcagc tgtggcaagg gcaagttgtg caagccaaga agttcagtaa
     1741 tgtcaccatg ctcttctcag acatcgttgg gttcactgcc atctgctccc agtgctcacc
     1801 gctgcaggtc atcaccatgc tcaatgcact gtacactcgc ttcgaccagc agtgtggaga
     1861 gctggatgtc tacaaggtgg agaccattgg cgatgcctat tgtgtagctg ggggattaca
     1921 caaagagagt gatactcatg ctgttcagat agcgctgatg gccctgaaga tgatggagct
     1981 ctctgatgaa gttatgtctc cccatggaga acctatcaag atgcgaattg gactgcactc
     2041 tggatcagtt tttgctggcg tcgttggagt taaaatgccc cgttactgtc tttttggaaa
     2101 caatgtcact ctggctaaca aatttgagtc ctgcagtgta ccacgaaaaa tcaatgtcag
     2161 cccaacaact tacagattac tcaaagactg tcctggtttc gtgtttaccc ctcgatcaag
     2221 ggaggaactt ccaccaaact tccctagtga aatccccgga atctgccatt ttctggatgc
     2281 ttaccaacaa ggaacaaact caaaaccatg cttccaaaag aaagatgtgg aagatggcaa
     2341 tgccaatttt ttaggcaaag catcaggaat agattagcaa cctatatacc tatttataag
     2401 tctttggggt ttgactcatt gaagatgtgt agagcctctg aaagcacttt agggattgta
     2461 gatggctaac aagcagtatt aaaatttcag gagccaagtc acaatctttc tcctgtttaa
     2521 catgacaaaa tgtatgtact cacttcagta cttcagctct tcaagaaaaa aaaaaaaaac
     2581 cttaaaaagc tacttttgtg ggagtatttc tattatataa ccagcactta ctacctgtac
     2641 tcaaaattca gcaccttgta catatatcag ataattgtag tcaattgtac aaactgatgg
     2701 agtcacctgc aatctcatat cctggtggaa tgccatggtt attaaagtgt gtttgtgata
     2761 gtgtcgtcaa aaaaaaggtt tttgaataga aattacattc tttgacaccc tcagttcttt
     2821 caaactttca actctgcatt ttattgcatt ttctcattta actgttagta tatgtttgaa
     2881 tataggctga ctttttttct ctattgcact ttcttctttt tttcttttta gaaaataagc
     2941 aattaggggt tagatgcaat atgaatataa aatagttctg taaataccaa atattatttt
     3001 tagttctgga agttaaaaat actctccata gtaacgtgtg ttataataat aaatatttct
     3061 tttgtttgac ttttttaaaa ttctacaatg gtaaaagcta ttttaatcca tgttagcaat
     3121 ttaattccta ttaattcatt ttactatcat aatagcaaag tattcagaat aaaagaggtt
     3181 tatatcttct attaaaatgc agtaatacta ttcaaatcta atttagctgg aagcaacatg
     3241 ggagtaaagt aatcatccag gggacccacc ataaaggaca ttggtaaaac atttacacac
     3301 gtaaacacac gtgcaggcac acttggaaca atatggaccc acttcagcag acatcttcta
     3361 ttgccatgct gagctgttta aagttttaag aagaaaatct aagtatggaa gtagaaaata
     3421 agacttgttc ttttggtgga tatctcattt ttcttgagta tttgtgctga acattttggt
     3481 gtatgtctat gaactaagaa tcaagttttt gtctcattaa atatacatat atgaagtgtt
     3541 tttattctac ttgaaatatc ttgacttaaa aaaaacgact gttagtattg atgaagcaaa
     3601 tgaggcagtg actacattat ggaaatgtct aagtattttt tccggaactt caactttttc
     3661 tttttgtttt acataaacat ttaagcagct aggaactttt agttttagtt aggtcagttg
     3721 aagttctctg gtgaactaaa aaatctacat tttggttggg gataaagatt tgattttaaa
     3781 aacagcttga atttaaagca agaaaaagat gaacgttata gttttaaaat caatattagc
     3841 agagccatag ataggctgag ctcttatttg gggttctgag aaataactca gtttaaaact
     3901 tctttaactg cctcccttag tacccaggct agcccatggt gacatcaggg atattcatta
     3961 ctgattggcc tctagtattc caagggtact actatagata ttacaatgat gctgtttact
     4021 aagtgataac cccgtatctt acaggactga ctttgactca ataaaccact cagcctatta
     4081 ttggaaagtg ccataggcct tgagaaagta tagttacatc ctcacaaata tatgaagtta
     4141 agtcactact tacaaggaaa aaaaatgtaa atacattttt ctttctggaa ccgaactctt
     4201 attatgggga aataatagaa ataaagaaaa tgtaaaagca tgaaatggaa tgtagtcttg
     4261 atcaaacatt tcagcagacc tcaatttgct tacaatctgt actcaaaaag tttataattc
     4321 aatcagaatt caaaaagtat tttgtgtgtt tgtgatgtat tagcatatac acaaaacaaa
     4381 tgaggggaat gtatataatc ttggtgaaaa gttgtatggg agttttttgt acgattcctg
     4441 caacttttct ataaacgtgg cattatatct aaataaaaaa ttaccaaaaa acatattttc
     4501 acagtgaagt taaatcatct gtgataggtt ccccagacca gcctacaaag gcccattaaa
     4561 tacagaataa tttgtggatc ttcaaagagg atgaacatac atttccatga cagagtccat
     4621 acgaaattca caatttagat gctaaaaaat ttaaggaata tctttttttc tctatcttat
     4681 ctgctttgaa gcatagcgat tagcgagaaa cttttcagat ccagaccact gatggtagcc
     4741 agagtagaga ccctggagca tagattctta aaataaataa ggggcccaag tcagagcttt
     4801 gaggtattga agatggaaaa atagtggaca aacagcctgt aatcaggttg gggattacgg
     4861 gtactgagtt atgttgccat ttaactattc taaaaaataa gactgggtct caagactgct
     4921 tccctaacag actgtttctc ctgaaaatgc aggagctatt ctttctgttc tggttatata
     4981 gtttccattc atggcttctt gtcctgttgt tttttgtgtc gagtacacta tataaattat
     5041 ctccttatac atatttctca ggcaagcaca gagttatact gaacttttct aaagatgctt
     5101 tgcatcaaga agcaaaggga aatacagaat taaaatgttt ctttccattt tgctttgttt
     5161 ttctatatcg atctagactt tgtaggaaaa tgcaaagcgt atatttaaga aaacctaaat
     5221 aagaatagat tcatttactc attttcattt attcattcat gaagaattta ttgaatgcct
     5281 actatgtgcc aggaatattg ctatattttt gaaatttaag gataaaatat ggcccaatct
     5341 atcaagaaag gaaagtgaaa gacaaatgta agacataatt acaacattct gtgggagcac
     5401 aaagtagggg actcagttct tagagaagtc ttttggtctt agagttcatc aagcagagaa
     5461 ggggaaggac atggtccagg aaaataaatc agcacacaaa gagatggggt cttgagggtg
     5521 catagtacat tctggacagc cagttacctg ggatgagttg ggagggagga gaataaggac
     5581 aaaagaccat ctgggcaaaa atcacgaagg ggtatgtgtg tcatgtaaag gtgtgccatg
     5641 atagttattc atattgctat tgtaatatta atatatagta attaactaca catgacacag
     5701 ctttacatga ccttaagtag ttatcaacat taccataata gtaatattaa taactacaat
     5761 aagagccatt attattcact tgaggcactt gttcaaaata gattttacaa aagaaaaaaa
     5821 aaaaagaagc aacatgagta aaattagatc cattggctac tgactccctc tccaacagtt
     5881 ttaaagctac tagataagag aagcatcaag tcttaaagga agagaatggt atgatcagat
     5941 gtgtgttttc caaagactat tagaaacagc atggttaagg atagggtgga tatttacttg
     6001 cacaggtgac tgaatgaaca tctgtttata catcaaggga aaatgattca ttttatatcc
     6061 tggatatttt accataagta tgagcataaa gctgggatgt ctggcattgg aattttatgt
     6121 cactctcaag gctaattagt ctttcagatc ctttggtagg gaggaaagaa gagttgcttc
     6181 agcattttga ctaacatttc ttccaaattt cttctccctg atatttccag ccacgccaag
     6241 gcaaagactt cccagctctg tctcaggtta ggttaaaaga aggcatagga tggaagaggg
     6301 tggtctgtgg taggcctagc aaatgaaaga gatgaataaa caagagtgtc tagtctaact
     6361 agtgcctgag gaatagacat cagtcctctg agattaccca gctggaaaac tgcccagatg
     6421 gagctttaca tgcaaagctc cagtgaagtc tagtgctgta taatgcttta aatccacaat
     6481 caagcccttg ttgatgcaaa tgttagtcgc ccaaagcaga atgggttaac gaattactgc
     6541 agtggtttta ggtgcatggg tttgacccat gtaaatcatt ttaataagta aaattgaatc
     6601 tttgatacat atttgttgag gagcttgtca gatcactacc tttcactgaa cagtaaaaca
     6661 tggatcattt atttcacctc attttgcact tcaatgcaat ttcactcttt aggtggttgt
     6721 acttggacag ttaaatgaag actgtcttta agggtgcagg gatggagaac acagcatata
     6781 tgagaggtgg gagggcagag acgccttgcg attcctgtga ggagggactt accgtctcct
     6841 ccaaaacaga aattcagtgc tgcagagacg gtagcaaagt gcagttctct tgttaaaaca
     6901 caggctaaaa gcagtgactg cttaacttat taagcagaga aaaccaaaat gtcattttca
     6961 accaaaattg agtgttagag tttatggtct ctgttatgat acttttcaag acctgggaaa
     7021 tatttgtatc ctttagtgaa aaggggaaga atcaattact gaaagccata agcttacagt
     7081 aacggagcca attaatcctt tgtcatcttt gttgagttta gttttcttct gttcttagca
     7141 cgtcagctag tagatctttg atgaactgtt gctcctcttt aaatgtttct tttattatat
     7201 tgatgatgtg cactcattca gtattcttat tttaagctgc agtaatcact gtacatttga
     7261 ttgccatctg tacttccctt gaagcagctt tgcttattac agccacacct gcatctgttt
     7321 tgactagccc acataaccta cacatttttc atccaaagct atcccattac cttatttatg
     7381 tccatttatt ttcatatata gtatctgcca aaatagtagc aaaagtttta tcaatgtgta
     7441 gatttcaatc agaaaaacct aagcaaaagg aaaaaaaagt gatattctaa aagccacact
     7501 tcttttatca ccagtgcagt tttttccatc atcataaact tagttttatg aaaatgttaa
     7561 tatcttggat ttaaacattg gggtagaaat caggacaaaa tttaaatgtc atatccagga
     7621 cttaatctca gatattctgg tagaagatat ttagataagg ttgtggctgc ctgtcaagtt
     7681 ttttaatttt ttaaattata taatgtcaga aaattaaatc acaaatgagc cttccagatt
     7741 gtgaggaaaa ttacttggct gaaaatgact ttgcataatg cccaaatctt gaagtatctg
     7801 ctcagatgtt tgttccatct gaaatgctgc caaatccatc tgtattgagg aaagagctat
     7861 gaggtagttt cagagaaagt gaaaacaaaa caaagcaaaa caaaaatgct attacagaag
     7921 ttggacagcc caatgtgtta agtattatac acctggaaca agagcagcaa aactgaagct
     7981 ctttggaaag ttatggcaac agtggtctgg gaattctggg cctttagaaa taatcattcc
     8041 cagtgataca gggatgtgca gcatccagac actgcttaat cacagtgagc tgccgtcttc
     8101 cctgaataag ttgtatctta taacattgaa gtgaatgaaa atgtattagt atttgagaat
     8161 ttactattcc ttcacctcct tgcacatgta aattaaagat gttttggaaa atatgagaac
     8221 ttgatgttca ctgccctaga gcactattct ccaacctttt agattcctgc aatctgttaa
     8281 taaaatattg taggacaaca actctataga ctgatacatg ctgctctcaa tccatatact
     8341 acatattagg acagcttttc ttttctaaat gagcatgaat ataaatagaa attctagtat
     8401 tttcttcacc taccccagtg aatcatcttt atattttact tgggaaactg ctacagttgg
     8461 ctagtgggtc ttcactttta ttgctttgag aaatggataa aagacataga ctatcacccc
     8521 tccaaaaatg tgcatatggc cccatacaag acacatgtac tgccagagct tggggaactc
     8581 cctagaatct actcctagac caccctagtg aagtaccata gaccatattg tctagatggg
     8641 acaaaataat aaattttgac taagaactta ctgtttagag agaaaaaata gtaaataatg
     8701 ttcaaaatga gaagacaata actgacaagt ctgtgatttt tgggttagat gagaccactt
     8761 ttgatttttg aggaataaaa ggagttagtt ttttggtcct gatgtgtaaa atgattcatg
     8821 gggtttcaca tacagacaag tagttttctt ttcagcaaat atttaacata tctctcaata
     8881 tatttttcac cagtttttgg taaatatgca ttgctatagg tataccaagt tttttttgtt
     8941 atgtttagta actaatttca ttttttctgt atatgtaaat atagctattg gaccctgcat
     9001 tgaaaacctt cttagtacta acaacactcc ttttattatt accttcatag ttactttaaa
     9061 tgacatattt atttcgagtt ttacttctgg ctgagttgca aagtctatga gaatgttacc
     9121 tcctgtctca gaagcttgtt agttattgtg ccacctaaat attgtaataa aagtgtttaa
     9181 ataaaaatct tcttaaaatt taaaa
//
