LOCUS       NM_033553                597 bp    mRNA    linear   PRI 01-JUN-2024
DEFINITION  Homo sapiens guanylate cyclase activator 2A (GUCA2A), mRNA.
ACCESSION   NM_033553 XM_046404
VERSION     NM_033553.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 597)
  AUTHORS   Blomain,E.S., Rappaport,J.A., Pattison,A.M., Bashir,B.,
            Caparosa,E., Stem,J., Snook,A.E. and Waldman,S.A.
  TITLE     APC-beta-catenin-TCF signaling silences the intestinal
            guanylin-GUCY2C tumor suppressor axis
  JOURNAL   Cancer Biol Ther 21 (5), 441-451 (2020)
   PUBMED   32037952
  REMARK    GeneRIF: APC-beta-catenin-TCF signaling silences the intestinal
            guanylin-GUCY2C tumor suppressor axis.
REFERENCE   2  (bases 1 to 597)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 597)
  AUTHORS   Fernandez-Cachon,M.L., Pedersen,S.L., Rigbolt,K.T., Zhang,C.,
            Fabricius,K., Hansen,H.H., Elster,L., Fink,L.N., Schafer,M.,
            Rhee,N.A., Langholz,E., Wandall,E., Friis,S.U., Vilmann,P.,
            Kristiansen,V.B., Schmidt,C., Schreiter,K., Breitschopf,K.,
            Hubschle,T., Jorsal,T., Vilsboll,T., Schmidt,T., Theis,S.,
            Knop,F.K., Larsen,P.J. and Jelsing,J.
  TITLE     Guanylin and uroguanylin mRNA expression is increased following
            Roux-en-Y gastric bypass, but guanylins do not play a significant
            role in body weight regulation and glycemic control
  JOURNAL   Peptides 101, 32-43 (2018)
   PUBMED   29289697
  REMARK    GeneRIF: Guanylin and uroguanylin, as well as their prohormones, do
            not seem to play a significant role in body weight regulation and
            glycemic control, suggesting that guanylin-family peptides do not
            show promise as targets for the treatment of obesity or diabetes.
REFERENCE   4  (bases 1 to 597)
  AUTHORS   Rodriguez,A., Gomez-Ambrosi,J., Catalan,V., Ezquerro,S.,
            Mendez-Gimenez,L., Becerril,S., Ibanez,P., Vila,N., Margall,M.A.,
            Moncada,R., Valenti,V., Silva,C., Salvador,J. and Fruhbeck,G.
  TITLE     Guanylin and uroguanylin stimulate lipolysis in human visceral
            adipocytes
  JOURNAL   Int J Obes (Lond) 40 (9), 1405-1415 (2016)
   PUBMED   27108812
  REMARK    GeneRIF: Both guanylin and uroguanylin trigger lipolysis in human
            visceral adipocytes. Given the lipolytic action of the guanylin
            system on visceral adipocytes, the herein reported decrease of
            circulating prouroguanylin concentrations in obese patients may
            have a role in excessive fat accumulation in obesity.
REFERENCE   5  (bases 1 to 597)
  AUTHORS   Lan,D., Niu,J., Miao,J., Dong,X., Wang,H., Yang,G., Wang,K. and
            Miao,Y.
  TITLE     Expression of guanylate cyclase-C, guanylin, and uroguanylin is
            downregulated proportionally to the ulcerative colitis disease
            activity index
  JOURNAL   Sci Rep 6, 25034 (2016)
   PUBMED   27125248
  REMARK    GeneRIF: Results demonstrate that GC-C and its ligands, guanylin
            and uroguanylin are downregulated in ulcerative colitis (UC), and
            this downregulation is more significant with aggravation of the
            clinical condition. Therefore, the GC-C signaling pathway may be
            implicated in the progression of UC.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 597)
  AUTHORS   Adams,M.D., Kerlavage,A.R., Fleischmann,R.D., Fuldner,R.A.,
            Bult,C.J., Lee,N.H., Kirkness,E.F., Weinstock,K.G., Gocayne,J.D.,
            White,O. et al.
  TITLE     Initial assessment of human gene diversity and expression patterns
            based upon 83 million nucleotides of cDNA sequence
  JOURNAL   Nature 377 (6547 Suppl), 3-174 (1995)
   PUBMED   7566098
REFERENCE   7  (bases 1 to 597)
  AUTHORS   Sciaky,D., Jenkins,N.A., Gilbert,D.J., Copeland,N.G., Sonoda,G.,
            Testa,J.R. and Cohen,M.B.
  TITLE     Mapping of guanylin to murine chromosome 4 and human chromosome
            1p34-p35
  JOURNAL   Genomics 26 (2), 427-429 (1995)
   PUBMED   7601480
REFERENCE   8  (bases 1 to 597)
  AUTHORS   Hill,O., Kuhn,M., Zucht,H.D., Cetin,Y., Kulaksiz,H., Adermann,K.,
            Klock,G., Rechkemmer,G., Forssmann,W.G. and Magert,H.J.
  TITLE     Analysis of the human guanylin gene and the processing and cellular
            localization of the peptide
  JOURNAL   Proc Natl Acad Sci U S A 92 (6), 2046-2050 (1995)
   PUBMED   7892222
REFERENCE   9  (bases 1 to 597)
  AUTHORS   Wiegand,R.C., Kato,J., Huang,M.D., Fok,K.F., Kachur,J.F. and
            Currie,M.G.
  TITLE     Human guanylin: cDNA isolation, structure, and activity
  JOURNAL   FEBS Lett 311 (2), 150-154 (1992)
   PUBMED   1327879
REFERENCE   10 (bases 1 to 597)
  AUTHORS   de Sauvage,F.J., Keshav,S., Kuang,W.J., Gillett,N., Henzel,W. and
            Goeddel,D.V.
  TITLE     Precursor structure, expression, and tissue distribution of human
            guanylin
  JOURNAL   Proc Natl Acad Sci U S A 89 (19), 9089-9093 (1992)
   PUBMED   1409606
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC114492.6, T28160.1 and M97496.1.
            
            On Nov 23, 2018 this sequence version replaced NM_033553.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: ERR279846.619.1, ERR279843.4380.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968540, SAMEA2142348
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000357001.3/ ENSP00000349493.2
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-21                AC114492.6         101947-101967       c
            22-82               T28160.1           2-62
            83-596              M97496.1           58-571
            597-597             AC114492.6         99912-99912         c
FEATURES             Location/Qualifiers
     source          1..597
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p34.2"
     gene            1..597
                     /gene="GUCA2A"
                     /gene_synonym="GCAP-I; GUCA2; STARA"
                     /note="guanylate cyclase activator 2A"
                     /db_xref="GeneID:2980"
                     /db_xref="HGNC:HGNC:4682"
                     /db_xref="MIM:139392"
     exon            1..108
                     /gene="GUCA2A"
                     /gene_synonym="GCAP-I; GUCA2; STARA"
                     /inference="alignment:Splign:2.1.0"
     CDS             34..381
                     /gene="GUCA2A"
                     /gene_synonym="GCAP-I; GUCA2; STARA"
                     /note="guanylate cyclase activator 2A (guanylin 2,
                     intestinal, heat-stable); guanylate cyclase-activating
                     protein 1; guanylate cyclase-activating protein I;
                     prepro-guanylin"
                     /codon_start=1
                     /product="guanylin precursor"
                     /protein_id="NP_291031.2"
                     /db_xref="CCDS:CCDS465.1"
                     /db_xref="GeneID:2980"
                     /db_xref="HGNC:HGNC:4682"
                     /db_xref="MIM:139392"
                     /translation="MNAFLLSALCLLGAWAALAGGVTVQDGNFSFSLESVKKLKDLQE
                     PQEPRVGKLRNFAPIPGEPVVPILCSNPNFPEELKPLCKEPNAQEILQRLEEIAEDPG
                     TCEICAYAACTGC"
     exon            109..316
                     /gene="GUCA2A"
                     /gene_synonym="GCAP-I; GUCA2; STARA"
                     /inference="alignment:Splign:2.1.0"
     exon            317..597
                     /gene="GUCA2A"
                     /gene_synonym="GCAP-I; GUCA2; STARA"
                     /inference="alignment:Splign:2.1.0"
     regulatory      577..582
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GUCA2A"
                     /gene_synonym="GCAP-I; GUCA2; STARA"
                     /note="hexamer: AATAAA"
     polyA_site      597
                     /gene="GUCA2A"
                     /gene_synonym="GCAP-I; GUCA2; STARA"
                     /note="major polyA site"
ORIGIN      
        1 agaacagcca cccctctctc gggcactgct gccatgaatg ccttcctgct ctccgcactg
       61 tgcctccttg gggcctgggc cgccttggca ggaggggtca ccgtgcagga tggaaatttc
      121 tccttttctc tggagtcagt gaagaagctc aaagacctcc aggagcccca ggagcccagg
      181 gttgggaaac tcaggaactt tgcacccatc cctggtgaac ctgtggttcc catcctctgt
      241 agcaacccga actttccaga agaactcaag cctctctgca aggagcccaa tgcccaggag
      301 atacttcaga ggctggagga aatcgctgag gacccgggca catgtgaaat ctgtgcctac
      361 gctgcctgta ccggatgcta ggggggcttg cccactgcct gcctcccctc cgcagcaggg
      421 aagctctttt ctcctgcaga aagggccacc catgatactc cactcccagc agctcaacct
      481 accctggtcc agtcgggagg agcagcccgg ggaggaactg ggtgactgga ggcctcgccc
      541 caacactgtc cttccctgcc acttcaaccc ccagctaata aaccagattc cagagta
//
