LOCUS       NM_001271867            3247 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens general transcription factor IIH subunit 3 (GTF2H3),
            transcript variant 3, mRNA.
ACCESSION   NM_001271867
VERSION     NM_001271867.2
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3247)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   2  (bases 1 to 3247)
  AUTHORS   Radu,L., Schoenwetter,E., Braun,C., Marcoux,J., Koelmel,W.,
            Schmitt,D.R., Kuper,J., Cianferani,S., Egly,J.M., Poterszman,A. and
            Kisker,C.
  TITLE     The intricate network between the p34 and p44 subunits is central
            to the activity of the transcription/DNA repair factor TFIIH
  JOURNAL   Nucleic Acids Res 45 (18), 10872-10883 (2017)
   PUBMED   28977422
  REMARK    GeneRIF: interface variants between the p34 and p44 subunits only
            mildly affected the association between the full length proteins
            and did not impinge on TFIIH activities due to the presence of an
            additional interface involving the C4 domain of p34.
REFERENCE   3  (bases 1 to 3247)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Paulo,J.A., Cannon,J.R., Ting,L.,
            Baltier,K., Colby,G., Gebreab,F., Gygi,M.P., Parzen,H., Szpyt,J.,
            Tam,S., Zarraga,G., Pontano-Vaites,L., Swarup,S., White,A.E.,
            Schweppe,D.K., Rad,R., Erickson,B.K., Obar,R.A., Guruharsha,K.G.,
            Li,K., Artavanis-Tsakonas,S., Gygi,S.P. and Harper,J.W.
  TITLE     Architecture of the human interactome defines protein communities
            and disease networks
  JOURNAL   Nature 545 (7655), 505-509 (2017)
   PUBMED   28514442
REFERENCE   4  (bases 1 to 3247)
  AUTHORS   He,Y., Yan,C., Fang,J., Inouye,C., Tjian,R., Ivanov,I. and
            Nogales,E.
  TITLE     Near-atomic resolution visualization of human transcription
            promoter opening
  JOURNAL   Nature 533 (7603), 359-365 (2016)
   PUBMED   27193682
REFERENCE   5  (bases 1 to 3247)
  AUTHORS   Marcon,E., Ni,Z., Pu,S., Turinsky,A.L., Trimble,S.S., Olsen,J.B.,
            Silverman-Gavrila,R., Silverman-Gavrila,L., Phanse,S., Guo,H.,
            Zhong,G., Guo,X., Young,P., Bailey,S., Roudeva,D., Zhao,D.,
            Hewel,J., Li,J., Graslund,S., Paduch,M., Kossiakoff,A.A.,
            Lupien,M., Emili,A., Wodak,S.J. and Greenblatt,J.
  TITLE     Human-chromatin-related protein interactions identify a demethylase
            complex required for chromosome segregation
  JOURNAL   Cell Rep 8 (1), 297-310 (2014)
   PUBMED   24981860
REFERENCE   6  (bases 1 to 3247)
  AUTHORS   Blau,J., Xiao,H., McCracken,S., O'Hare,P., Greenblatt,J. and
            Bentley,D.
  TITLE     Three functional classes of transcriptional activation domain
  JOURNAL   Mol Cell Biol 16 (5), 2044-2055 (1996)
   PUBMED   8628270
REFERENCE   7  (bases 1 to 3247)
  AUTHORS   Tong,X., Drapkin,R., Reinberg,D. and Kieff,E.
  TITLE     The 62- and 80-kDa subunits of transcription factor IIH mediate the
            interaction with Epstein-Barr virus nuclear protein 2
  JOURNAL   Proc Natl Acad Sci U S A 92 (8), 3259-3263 (1995)
   PUBMED   7724549
REFERENCE   8  (bases 1 to 3247)
  AUTHORS   Humbert,S., van Vuuren,H., Lutz,Y., Hoeijmakers,J.H., Egly,J.M. and
            Moncollin,V.
  TITLE     p44 and p34 subunits of the BTF2/TFIIH transcription factor have
            homologies with SSL1, a yeast protein involved in DNA repair
  JOURNAL   EMBO J 13 (10), 2393-2398 (1994)
   PUBMED   8194529
REFERENCE   9  (bases 1 to 3247)
  AUTHORS   Schaeffer,L., Roy,R., Humbert,S., Moncollin,V., Vermeulen,W.,
            Hoeijmakers,J.H., Chambon,P. and Egly,J.M.
  TITLE     DNA repair helicase: a component of BTF2 (TFIIH) basic
            transcription factor
  JOURNAL   Science 260 (5104), 58-63 (1993)
   PUBMED   8465201
REFERENCE   10 (bases 1 to 3247)
  AUTHORS   Gerard,M., Fischer,L., Moncollin,V., Chipoulet,J.M., Chambon,P. and
            Egly,J.M.
  TITLE     Purification and interaction properties of the human RNA polymerase
            B(II) general transcription factor BTF2
  JOURNAL   J Biol Chem 266 (31), 20940-20945 (1991)
   PUBMED   1939143
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK298128.1, BC016767.1,
            BC013216.1, BC039726.2, AC117503.8, CB955773.1, BM013501.1 and
            CA306634.1.
            
            On May 31, 2019 this sequence version replaced NM_001271867.1.
            
            Summary: This gene encodes a member of the TFB4 family. The encoded
            protein is a subunit of the core-TFIIH basal transcription factor
            and localizes to the nucleus. The encoded protein is involved in
            RNA transcription by RNA polymerase II and nucleotide excision
            repair and associates with the Cdk-activating kinase complex.
            Alternative splicing results in multiple transcript variants. A
            related pseudogene has been identified on chromosome 14. [provided
            by RefSeq, Dec 2012].
            
            Transcript Variant: This variant (3) lacks an exon in the central
            coding region and initiates translation at an alternate start
            codon, compared to variant 1. The encoded isoform (c) has a shorter
            N-terminus compared to isoform a.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK298128.1, SRR1163658.390.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-925               AK298128.1         1-925
            926-1424            BC016767.1         1001-1499
            1425-1472           AK298128.1         1425-1472
            1473-1792           BC013216.1         279-598
            1793-2295           BC039726.2         1829-2331
            2296-2345           AC117503.8         164807-164856       c
            2346-2451           CB955773.1         1-106
            2452-2525           BM013501.1         542-615
            2526-2781           CB955773.1         181-436
            2782-3247           CA306634.1         18-483              c
FEATURES             Location/Qualifiers
     source          1..3247
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12q24.31"
     gene            1..3247
                     /gene="GTF2H3"
                     /gene_synonym="BTF2; P34; TFB4; TFIIH"
                     /note="general transcription factor IIH subunit 3"
                     /db_xref="GeneID:2967"
                     /db_xref="HGNC:HGNC:4657"
                     /db_xref="MIM:601750"
     exon            1..44
                     /gene="GTF2H3"
                     /gene_synonym="BTF2; P34; TFB4; TFIIH"
                     /inference="alignment:Splign:2.1.0"
     exon            45..151
                     /gene="GTF2H3"
                     /gene_synonym="BTF2; P34; TFB4; TFIIH"
                     /inference="alignment:Splign:2.1.0"
     CDS             75..878
                     /gene="GTF2H3"
                     /gene_synonym="BTF2; P34; TFB4; TFIIH"
                     /note="isoform c is encoded by transcript variant 3; basic
                     transcription factor 2 34 kDa subunit; TFIIH basal
                     transcription factor complex p34 subunit; general
                     transcription factor IIH, polypeptide 3, 34kDa"
                     /codon_start=1
                     /product="general transcription factor IIH subunit 3
                     isoform c"
                     /protein_id="NP_001258796.1"
                     /db_xref="CCDS:CCDS61275.1"
                     /db_xref="GeneID:2967"
                     /db_xref="HGNC:HGNC:4657"
                     /db_xref="MIM:601750"
                     /translation="MVLGNSHLFMNRSNKLAVIASHIQESRFLYPGKNGRLGDFFGDP
                     GNPPEFNPSGSKDGKYELLTSANEVIVEEIKDLMTKSDIKGQHTETLLAGSLAKALCY
                     IHRMNKEVKDNQEMKSRILVIKAAEDSALQYMNFMNVIFAAQKQNILIDACVLDSDSG
                     LLQQACDITGGLYLKVPQMPSLLQYLLWVFLPDQDQRSQLILPPPVHVDYRAACFCHR
                     NLIEIGYVCSVCLSIFCNFSPICTTCETAFKISLPPVLKAKKKKLKVSA"
     exon            152..315
                     /gene="GTF2H3"
                     /gene_synonym="BTF2; P34; TFB4; TFIIH"
                     /inference="alignment:Splign:2.1.0"
     exon            316..378
                     /gene="GTF2H3"
                     /gene_synonym="BTF2; P34; TFB4; TFIIH"
                     /inference="alignment:Splign:2.1.0"
     exon            379..408
                     /gene="GTF2H3"
                     /gene_synonym="BTF2; P34; TFB4; TFIIH"
                     /inference="alignment:Splign:2.1.0"
     exon            409..437
                     /gene="GTF2H3"
                     /gene_synonym="BTF2; P34; TFB4; TFIIH"
                     /inference="alignment:Splign:2.1.0"
     exon            438..512
                     /gene="GTF2H3"
                     /gene_synonym="BTF2; P34; TFB4; TFIIH"
                     /inference="alignment:Splign:2.1.0"
     exon            513..566
                     /gene="GTF2H3"
                     /gene_synonym="BTF2; P34; TFB4; TFIIH"
                     /inference="alignment:Splign:2.1.0"
     exon            567..635
                     /gene="GTF2H3"
                     /gene_synonym="BTF2; P34; TFB4; TFIIH"
                     /inference="alignment:Splign:2.1.0"
     exon            636..771
                     /gene="GTF2H3"
                     /gene_synonym="BTF2; P34; TFB4; TFIIH"
                     /inference="alignment:Splign:2.1.0"
     exon            772..808
                     /gene="GTF2H3"
                     /gene_synonym="BTF2; P34; TFB4; TFIIH"
                     /inference="alignment:Splign:2.1.0"
     exon            809..3247
                     /gene="GTF2H3"
                     /gene_synonym="BTF2; P34; TFB4; TFIIH"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3091..3096
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GTF2H3"
                     /gene_synonym="BTF2; P34; TFB4; TFIIH"
                     /note="hexamer: ATTATA"
     polyA_site      3108
                     /gene="GTF2H3"
                     /gene_synonym="BTF2; P34; TFB4; TFIIH"
                     /note="major polyA site"
     regulatory      3227..3232
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GTF2H3"
                     /gene_synonym="BTF2; P34; TFB4; TFIIH"
                     /note="hexamer: AATAAA"
     polyA_site      3247
                     /gene="GTF2H3"
                     /gene_synonym="BTF2; P34; TFB4; TFIIH"
                     /note="major polyA site"
ORIGIN      
        1 acttgctctg cgctgaggtg ctgggacagc catggtttca gacgttcact ttatccaaat
       61 gcatagatgc cgtgatggtg ctgggaaatt cgcatttatt catgaatcgt tccaacaaac
      121 ttgctgtgat agcaagtcac attcaagaaa gccgattctt atatcctgga aagaatggca
      181 gacttggaga cttcttcgga gaccctggca accctcctga atttaatccc tctgggagta
      241 aagatggaaa atacgaactt ttaacctcag caaatgaagt tattgttgaa gagattaaag
      301 atctaatgac caaaagtgac ataaagggtc aacatacaga aactttgctg gcaggatccc
      361 tggccaaagc cctttgctac attcatagaa tgaacaagga agttaaagac aatcaggaaa
      421 tgaaatcaag gatattggtg attaaggctg cagaagacag tgcgttgcag tatatgaact
      481 tcatgaatgt catctttgca gcacagaaac agaatatttt gattgatgcc tgtgttttag
      541 actccgactc agggctcctc caacaggctt gtgacatcac gggaggactg tacctgaagg
      601 tgcctcagat gccttctctt ctgcagtatt tgctgtgggt gtttcttccc gatcaagatc
      661 agagatctca gttaatcctc ccacccccag ttcatgttga ctacagggct gcttgcttct
      721 gtcatcgaaa tctcattgaa attggttatg tctgttctgt gtgtttgtca atattctgca
      781 atttcagccc catttgtact acgtgcgaga cagcctttaa aatttctctg cctccagtgc
      841 tgaaagccaa gaaaaagaaa ctgaaagtgt ctgcctgagg ataaaatatt ttccccatct
      901 tttagagctg ttaatagaaa ttatatagca gattctttgt tgggaagact gaaaaaaata
      961 aagataggta taggataatt tttaatatgg tgaccttaca gaaaatattt cccaaacatc
     1021 cttttcatcc tgtgcttctg gaggactgat ttgtttgagg gaatcattct atgcattata
     1081 tcctaaaata ttctatgact ggtttctgtc catgtttgtg gctttcattt ttttaatggg
     1141 atgactatta gtcaaagtca gcttgtcatg actcatcata ggctttctaa cctactccct
     1201 gaatccgggt cctcattgtg aaatgcatgc catacgaaat ttgaacgtag ctttggaaaa
     1261 agggactatt tgtggagtaa tggcattaat caacatagaa catcttattt gaatcaacag
     1321 ttaacttcag tagtcatgtg aataaaattc ttattgtcta aattgagaca gcctcagata
     1381 tttgcagata tttacttttt gtctgatatc agtacatatt tggacaaagt catctaaata
     1441 atagtttgtc accaaataac tacaaaatct cattttaaat gagtaaggag aacgtgtaca
     1501 gaagcaaatt ttcttcaaaa tagttgtggg aagagcttat atgtgaaagc ttatgactgg
     1561 ttttgaggga gaacttactg gagaaaatgg actctatgtt aagtatggtt ttcagataga
     1621 attctttcct tttttaatga ggaaaaaaaa tccacattaa tattgaaact gcacctgtaa
     1681 tcccagcact ttgggaggct gaggacagag gattgcttga gcccaggagt tcgagagcag
     1741 cctgggcagc aaagtgagac cccatctcta ctaaaaattt aaatgtattt attaaaactg
     1801 ttctctagaa gctttggact gaatcccaaa agtgtttata agttcaaaag caaaagtatt
     1861 tgtaatttca acaacaaaaa atgtatttct ttatgtaatc ttgaaattat taaaagtcct
     1921 tttagcttct agcacatatt tgtacaaaga gtttaaggaa tggtggctgg tttggtttgt
     1981 tttttaaaaa tgtttactga cgaggccggg cgtggtggct cacccctgca atcccagcac
     2041 tttgggaggc cgaggcaggc agatcacaag gtcaggagtt caagatcagc ctggccagta
     2101 tggtgaaacc ctgtctctac taaaaataga aaaattagcc atgcgaagta gcaggtgcct
     2161 gtagttccag ctactcggga ggctgaggca agagaattgc ttgaatccag gaggcagagg
     2221 ttgcagtgag ccaagatagc gcctctgtac tccagcctgg gtgacagagc gagactctgt
     2281 atcaaaaaaa aaaaagatcg ggcacgttgg ctcacgcctg taatcccagc actttgggag
     2341 gccaaggtgg gtggatcacg aggtcaggag tgatcaggct gggtttctgt tttgttttgt
     2401 tttgtgagac agagtctcgc tctgttgccc aggctggggt gcagtggtgc aagctcggct
     2461 ctctgcaagc tctgtctcct gggttcacgc cattctcctg cctcagcctc ccaagtagct
     2521 gggattacta ctctactgaa cccatgaggc agaaatttga gaccatcctg cccaacgtgg
     2581 tgaaaacatg gtgaaaccct gtctctacta aaaatacaaa aattagctgg gcgtggtggc
     2641 aggtgcctgt aatcccagct actcaggaga ctgaggcagg agaattgctt gaacccagga
     2701 ggtggaggtt gcagtgagcc aagatcaaga ttgcgccatt gcactccagc ctgggcgaca
     2761 agaacaaaac tctgtctcaa aaaaaaaaaa aaaaaaaaag tctgtactga taaaacccat
     2821 tgtgtacaaa actttgtatg taaggaagat tttaattttc tctttataca agctgagtca
     2881 tatttaaata atttgatgtt ggcttagata atttcagata gattttatat tctggatttg
     2941 tgtttttgtt aacaaatata caaagacttt ggtgatcact ttgcaaatat ttgttaatcc
     3001 ttgagtttga gaacctgtct tttaaaaata atattttgta tactaattaa gtgtaatgga
     3061 aatcacaatt ttaagtctag gaaataaagt attatatata ctttcaaaca agctagcaag
     3121 gcttttatta ttactttttt atttgaaata tcttatatat ttggttagtt ctgtttaact
     3181 tgtttttaac tgttgccctt atgagttatt ttatataaat ttttacaata aaataatttg
     3241 attttca
//
