LOCUS       NM_001271866            3198 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens general transcription factor IIH subunit 3 (GTF2H3),
            transcript variant 2, mRNA.
ACCESSION   NM_001271866
VERSION     NM_001271866.2
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3198)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   2  (bases 1 to 3198)
  AUTHORS   Radu,L., Schoenwetter,E., Braun,C., Marcoux,J., Koelmel,W.,
            Schmitt,D.R., Kuper,J., Cianferani,S., Egly,J.M., Poterszman,A. and
            Kisker,C.
  TITLE     The intricate network between the p34 and p44 subunits is central
            to the activity of the transcription/DNA repair factor TFIIH
  JOURNAL   Nucleic Acids Res 45 (18), 10872-10883 (2017)
   PUBMED   28977422
  REMARK    GeneRIF: interface variants between the p34 and p44 subunits only
            mildly affected the association between the full length proteins
            and did not impinge on TFIIH activities due to the presence of an
            additional interface involving the C4 domain of p34.
REFERENCE   3  (bases 1 to 3198)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Paulo,J.A., Cannon,J.R., Ting,L.,
            Baltier,K., Colby,G., Gebreab,F., Gygi,M.P., Parzen,H., Szpyt,J.,
            Tam,S., Zarraga,G., Pontano-Vaites,L., Swarup,S., White,A.E.,
            Schweppe,D.K., Rad,R., Erickson,B.K., Obar,R.A., Guruharsha,K.G.,
            Li,K., Artavanis-Tsakonas,S., Gygi,S.P. and Harper,J.W.
  TITLE     Architecture of the human interactome defines protein communities
            and disease networks
  JOURNAL   Nature 545 (7655), 505-509 (2017)
   PUBMED   28514442
REFERENCE   4  (bases 1 to 3198)
  AUTHORS   He,Y., Yan,C., Fang,J., Inouye,C., Tjian,R., Ivanov,I. and
            Nogales,E.
  TITLE     Near-atomic resolution visualization of human transcription
            promoter opening
  JOURNAL   Nature 533 (7603), 359-365 (2016)
   PUBMED   27193682
REFERENCE   5  (bases 1 to 3198)
  AUTHORS   Marcon,E., Ni,Z., Pu,S., Turinsky,A.L., Trimble,S.S., Olsen,J.B.,
            Silverman-Gavrila,R., Silverman-Gavrila,L., Phanse,S., Guo,H.,
            Zhong,G., Guo,X., Young,P., Bailey,S., Roudeva,D., Zhao,D.,
            Hewel,J., Li,J., Graslund,S., Paduch,M., Kossiakoff,A.A.,
            Lupien,M., Emili,A., Wodak,S.J. and Greenblatt,J.
  TITLE     Human-chromatin-related protein interactions identify a demethylase
            complex required for chromosome segregation
  JOURNAL   Cell Rep 8 (1), 297-310 (2014)
   PUBMED   24981860
REFERENCE   6  (bases 1 to 3198)
  AUTHORS   Blau,J., Xiao,H., McCracken,S., O'Hare,P., Greenblatt,J. and
            Bentley,D.
  TITLE     Three functional classes of transcriptional activation domain
  JOURNAL   Mol Cell Biol 16 (5), 2044-2055 (1996)
   PUBMED   8628270
REFERENCE   7  (bases 1 to 3198)
  AUTHORS   Tong,X., Drapkin,R., Reinberg,D. and Kieff,E.
  TITLE     The 62- and 80-kDa subunits of transcription factor IIH mediate the
            interaction with Epstein-Barr virus nuclear protein 2
  JOURNAL   Proc Natl Acad Sci U S A 92 (8), 3259-3263 (1995)
   PUBMED   7724549
REFERENCE   8  (bases 1 to 3198)
  AUTHORS   Humbert,S., van Vuuren,H., Lutz,Y., Hoeijmakers,J.H., Egly,J.M. and
            Moncollin,V.
  TITLE     p44 and p34 subunits of the BTF2/TFIIH transcription factor have
            homologies with SSL1, a yeast protein involved in DNA repair
  JOURNAL   EMBO J 13 (10), 2393-2398 (1994)
   PUBMED   8194529
REFERENCE   9  (bases 1 to 3198)
  AUTHORS   Schaeffer,L., Roy,R., Humbert,S., Moncollin,V., Vermeulen,W.,
            Hoeijmakers,J.H., Chambon,P. and Egly,J.M.
  TITLE     DNA repair helicase: a component of BTF2 (TFIIH) basic
            transcription factor
  JOURNAL   Science 260 (5104), 58-63 (1993)
   PUBMED   8465201
REFERENCE   10 (bases 1 to 3198)
  AUTHORS   Gerard,M., Fischer,L., Moncollin,V., Chipoulet,J.M., Chambon,P. and
            Egly,J.M.
  TITLE     Purification and interaction properties of the human RNA polymerase
            B(II) general transcription factor BTF2
  JOURNAL   J Biol Chem 266 (31), 20940-20945 (1991)
   PUBMED   1939143
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL600169.1, DB170244.1,
            BC016767.1, BG720598.1, BC039726.2, AC117503.8, CB955773.1,
            BM013501.1 and CA306634.1.
            
            On May 31, 2019 this sequence version replaced NM_001271866.1.
            
            Summary: This gene encodes a member of the TFB4 family. The encoded
            protein is a subunit of the core-TFIIH basal transcription factor
            and localizes to the nucleus. The encoded protein is involved in
            RNA transcription by RNA polymerase II and nucleotide excision
            repair and associates with the Cdk-activating kinase complex.
            Alternative splicing results in multiple transcript variants. A
            related pseudogene has been identified on chromosome 14. [provided
            by RefSeq, Dec 2012].
            
            Transcript Variant: This variant (2) lacks two in-frame exons but
            maintains the reading frame, compared to variant 1. This variant
            encodes isoform b, which is shorter than isoform a.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803613.228613.1,
                                           SRR1803613.140407.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-16                AL600169.1         37-52
            17-129              DB170244.1         1-113
            130-517             BC016767.1         125-512
            518-586             DB170244.1         504-572
            587-634             BG720598.1         572-619
            635-2246            BC039726.2         720-2331
            2247-2296           AC117503.8         164807-164856       c
            2297-2402           CB955773.1         1-106
            2403-2476           BM013501.1         542-615
            2477-2732           CB955773.1         181-436
            2733-3198           CA306634.1         18-483              c
FEATURES             Location/Qualifiers
     source          1..3198
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12q24.31"
     gene            1..3198
                     /gene="GTF2H3"
                     /gene_synonym="BTF2; P34; TFB4; TFIIH"
                     /note="general transcription factor IIH subunit 3"
                     /db_xref="GeneID:2967"
                     /db_xref="HGNC:HGNC:4657"
                     /db_xref="MIM:601750"
     exon            1..44
                     /gene="GTF2H3"
                     /gene_synonym="BTF2; P34; TFB4; TFIIH"
                     /inference="alignment:Splign:2.1.0"
     CDS             32..829
                     /gene="GTF2H3"
                     /gene_synonym="BTF2; P34; TFB4; TFIIH"
                     /note="isoform b is encoded by transcript variant 2; basic
                     transcription factor 2 34 kDa subunit; TFIIH basal
                     transcription factor complex p34 subunit; general
                     transcription factor IIH, polypeptide 3, 34kDa"
                     /codon_start=1
                     /product="general transcription factor IIH subunit 3
                     isoform b"
                     /protein_id="NP_001258795.1"
                     /db_xref="GeneID:2967"
                     /db_xref="HGNC:HGNC:4657"
                     /db_xref="MIM:601750"
                     /translation="MVSDEDELNLLVIVVDANPIWWGKQALKESQFTLSKCIDAVMVL
                     GNSHLFMNRSNKLAVIASHIQESRFLYPGKNGRLGDFFGDPGNPPEFNPSGSKDGKYE
                     LLTSANEVIVEEIKDLMTKSDIKGQHTETLLAGSLAKALCYIHRMNKEVKDNQEMKSR
                     ILACDITGGLYLKVPQMPSLLQYLLWVFLPDQDQRSQLILPPPVHVDYRAACFCHRNL
                     IEIGYVCSVCLSIFCNFSPICTTCETAFKISLPPVLKAKKKKLKVSA"
     exon            45..124
                     /gene="GTF2H3"
                     /gene_synonym="BTF2; P34; TFB4; TFIIH"
                     /inference="alignment:Splign:2.1.0"
     exon            125..231
                     /gene="GTF2H3"
                     /gene_synonym="BTF2; P34; TFB4; TFIIH"
                     /inference="alignment:Splign:2.1.0"
     exon            232..395
                     /gene="GTF2H3"
                     /gene_synonym="BTF2; P34; TFB4; TFIIH"
                     /inference="alignment:Splign:2.1.0"
     exon            396..458
                     /gene="GTF2H3"
                     /gene_synonym="BTF2; P34; TFB4; TFIIH"
                     /inference="alignment:Splign:2.1.0"
     exon            459..488
                     /gene="GTF2H3"
                     /gene_synonym="BTF2; P34; TFB4; TFIIH"
                     /inference="alignment:Splign:2.1.0"
     exon            489..517
                     /gene="GTF2H3"
                     /gene_synonym="BTF2; P34; TFB4; TFIIH"
                     /inference="alignment:Splign:2.1.0"
     exon            518..586
                     /gene="GTF2H3"
                     /gene_synonym="BTF2; P34; TFB4; TFIIH"
                     /inference="alignment:Splign:2.1.0"
     exon            587..722
                     /gene="GTF2H3"
                     /gene_synonym="BTF2; P34; TFB4; TFIIH"
                     /inference="alignment:Splign:2.1.0"
     exon            723..759
                     /gene="GTF2H3"
                     /gene_synonym="BTF2; P34; TFB4; TFIIH"
                     /inference="alignment:Splign:2.1.0"
     exon            760..3198
                     /gene="GTF2H3"
                     /gene_synonym="BTF2; P34; TFB4; TFIIH"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3042..3047
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GTF2H3"
                     /gene_synonym="BTF2; P34; TFB4; TFIIH"
                     /note="hexamer: ATTATA"
     polyA_site      3059
                     /gene="GTF2H3"
                     /gene_synonym="BTF2; P34; TFB4; TFIIH"
                     /note="major polyA site"
     regulatory      3178..3183
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GTF2H3"
                     /gene_synonym="BTF2; P34; TFB4; TFIIH"
                     /note="hexamer: AATAAA"
     polyA_site      3198
                     /gene="GTF2H3"
                     /gene_synonym="BTF2; P34; TFB4; TFIIH"
                     /note="major polyA site"
ORIGIN      
        1 acttgctctg cgctgaggtg ctgggacagc catggtttca gacgaagatg aattgaatct
       61 tctggttatt gtagttgatg ccaacccaat ttggtgggga aagcaagcat taaaggaatc
      121 tcagttcact ttatccaaat gcatagatgc cgtgatggtg ctgggaaatt cgcatttatt
      181 catgaatcgt tccaacaaac ttgctgtgat agcaagtcac attcaagaaa gccgattctt
      241 atatcctgga aagaatggca gacttggaga cttcttcgga gaccctggca accctcctga
      301 atttaatccc tctgggagta aagatggaaa atacgaactt ttaacctcag caaatgaagt
      361 tattgttgaa gagattaaag atctaatgac caaaagtgac ataaagggtc aacatacaga
      421 aactttgctg gcaggatccc tggccaaagc cctttgctac attcatagaa tgaacaagga
      481 agttaaagac aatcaggaaa tgaaatcaag gatattggct tgtgacatca cgggaggact
      541 gtacctgaag gtgcctcaga tgccttctct tctgcagtat ttgctgtggg tgtttcttcc
      601 cgatcaagat cagagatctc agttaatcct cccaccccca gttcatgttg actacagggc
      661 tgcttgcttc tgtcatcgaa atctcattga aattggttat gtctgttctg tgtgtttgtc
      721 aatattctgc aatttcagcc ccatttgtac tacgtgcgag acagccttta aaatttctct
      781 gcctccagtg ctgaaagcca agaaaaagaa actgaaagtg tctgcctgag gataaaatat
      841 tttccccatc ttttagagct gttaatagaa attatatagc agattctttg ttgggaagac
      901 tgaaaaaaat aaagataggt ataggataat ttttaatatg gtgaccttac agaaaatatt
      961 tcccaaacat ccttttcatc ctgtgcttct ggaggactga tttgtttgag ggaatcattc
     1021 tatgcattat atcctaaaat attctatgac tggtttctgt ccatgtttgt ggctttcatt
     1081 tttttaatgg gatgactatt agtcaaagtc agcttgtcat gactcatcat aggctttcta
     1141 acctactccc tgaatccggg tcctcattgt gaaatgcatg ccatacgaaa tttgaacgta
     1201 gctttggaaa aagggactat ttgtggagta atggcattaa tcaacataga acatcttatt
     1261 tgaatcaaca gttaacttca gtagtcatgt gaataaaatt cttattgtct aaattgagac
     1321 agcctcagat atttgcagat atttactttt tgtctgatat cagtacatat ttggacaaag
     1381 tcatctaaat aatagtttgt caccaaataa ctacaaaatc tcattttaaa tgagtaagga
     1441 gaacgtgtac agaagcaaat tttcttcaaa atagttgtgg gaagagctta tatgtgaaag
     1501 cttatgactg gttttgaggg agaacttact ggagaaaatg gactctatgt taagtatggt
     1561 tttcagatag aattctttcc ttttttaatg aggaaaaaaa atccacatta atattgaaac
     1621 tgcacctgta atcccagcac tttgggaggc tgaggacaga ggattgcttg agcccaggag
     1681 ttcgagagca gcctgggcag caaagtgaga ccccatctct actaaaaatt taaatgtatt
     1741 tattaaaact gttctctaga agctttggac tgaatcccaa aagtgtttat aagttcaaaa
     1801 gcaaaagtat ttgtaatttc aacaacaaaa aatgtatttc tttatgtaat cttgaaatta
     1861 ttaaaagtcc ttttagcttc tagcacatat ttgtacaaag agtttaagga atggtggctg
     1921 gtttggtttg ttttttaaaa atgtttactg acgaggccgg gcgtggtggc tcacccctgc
     1981 aatcccagca ctttgggagg ccgaggcagg cagatcacaa ggtcaggagt tcaagatcag
     2041 cctggccagt atggtgaaac cctgtctcta ctaaaaatag aaaaattagc catgcgaagt
     2101 agcaggtgcc tgtagttcca gctactcggg aggctgaggc aagagaattg cttgaatcca
     2161 ggaggcagag gttgcagtga gccaagatag cgcctctgta ctccagcctg ggtgacagag
     2221 cgagactctg tatcaaaaaa aaaaaagatc gggcacgttg gctcacgcct gtaatcccag
     2281 cactttggga ggccaaggtg ggtggatcac gaggtcagga gtgatcaggc tgggtttctg
     2341 ttttgttttg ttttgtgaga cagagtctcg ctctgttgcc caggctgggg tgcagtggtg
     2401 caagctcggc tctctgcaag ctctgtctcc tgggttcacg ccattctcct gcctcagcct
     2461 cccaagtagc tgggattact actctactga acccatgagg cagaaatttg agaccatcct
     2521 gcccaacgtg gtgaaaacat ggtgaaaccc tgtctctact aaaaatacaa aaattagctg
     2581 ggcgtggtgg caggtgcctg taatcccagc tactcaggag actgaggcag gagaattgct
     2641 tgaacccagg aggtggaggt tgcagtgagc caagatcaag attgcgccat tgcactccag
     2701 cctgggcgac aagaacaaaa ctctgtctca aaaaaaaaaa aaaaaaaaaa gtctgtactg
     2761 ataaaaccca ttgtgtacaa aactttgtat gtaaggaaga ttttaatttt ctctttatac
     2821 aagctgagtc atatttaaat aatttgatgt tggcttagat aatttcagat agattttata
     2881 ttctggattt gtgtttttgt taacaaatat acaaagactt tggtgatcac tttgcaaata
     2941 tttgttaatc cttgagtttg agaacctgtc ttttaaaaat aatattttgt atactaatta
     3001 agtgtaatgg aaatcacaat tttaagtcta ggaaataaag tattatatat actttcaaac
     3061 aagctagcaa ggcttttatt attacttttt tatttgaaat atcttatata tttggttagt
     3121 tctgtttaac ttgtttttaa ctgttgccct tatgagttat tttatataaa tttttacaat
     3181 aaaataattt gattttca
//
