LOCUS       NM_001514               1599 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens general transcription factor IIB (GTF2B), mRNA.
ACCESSION   NM_001514
VERSION     NM_001514.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Santana,J.F., Collins,G.S., Parida,M., Luse,D.S. and Price,D.H.
  TITLE     Differential dependencies of human RNA polymerase II promoters on
            TBP, TAF1, TFIIB and XPB
  JOURNAL   Nucleic Acids Res 50 (16), 9127-9148 (2022)
   PUBMED   35947745
  REMARK    GeneRIF: Differential dependencies of human RNA polymerase II
            promoters on TBP, TAF1, TFIIB and XPB.
REFERENCE   2  (bases 1 to 1599)
  AUTHORS   Cai,F., Chen,S., Yu,X., Zhang,J., Liang,W., Zhang,Y., Chen,Y.,
            Chen,S., Hong,Y., Yan,W., Wang,W., Zhang,J. and Wu,Q.
  TITLE     Transcription factor GTF2B regulates AIP protein expression in
            growth hormone-secreting pituitary adenomas and influences tumor
            phenotypes
  JOURNAL   Neuro Oncol 24 (6), 925-935 (2022)
   PUBMED   34932801
  REMARK    GeneRIF: Transcription factor GTF2B regulates AIP protein
            expression in growth hormone-secreting pituitary adenomas and
            influences tumor phenotypes.
REFERENCE   3  (bases 1 to 1599)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   4  (bases 1 to 1599)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   5  (bases 1 to 1599)
  AUTHORS   Ly,E., Powell,A.E., Goodrich,J.A. and Kugel,J.F.
  TITLE     Release of Human TFIIB from Actively Transcribing Complexes Is
            Triggered upon Synthesis of 7- and 9-nt RNAs
  JOURNAL   J Mol Biol 432 (14), 4049-4060 (2020)
   PUBMED   32417370
  REMARK    GeneRIF: Release of Human TFIIB from Actively Transcribing
            Complexes Is Triggered upon Synthesis of 7- and 9-nt RNAs.
REFERENCE   6  (bases 1 to 1599)
  AUTHORS   Ing,N.H., Beekman,J.M., Tsai,S.Y., Tsai,M.J. and O'Malley,B.W.
  TITLE     Members of the steroid hormone receptor superfamily interact with
            TFIIB (S300-II)
  JOURNAL   J Biol Chem 267 (25), 17617-17623 (1992)
   PUBMED   1517211
REFERENCE   7  (bases 1 to 1599)
  AUTHORS   Jacob,G.A., Luse,S.W. and Luse,D.S.
  TITLE     Abortive initiation is increased only for the weakest members of a
            set of down mutants of the adenovirus 2 major late promoter
  JOURNAL   J Biol Chem 266 (33), 22537-22544 (1991)
   PUBMED   1939271
REFERENCE   8  (bases 1 to 1599)
  AUTHORS   Malik,S., Hisatake,K., Sumimoto,H., Horikoshi,M. and Roeder,R.G.
  TITLE     Sequence of general transcription factor TFIIB and relationships to
            other initiation factors
  JOURNAL   Proc Natl Acad Sci U S A 88 (21), 9553-9557 (1991)
   PUBMED   1946368
REFERENCE   9  (bases 1 to 1599)
  AUTHORS   Ha,I., Lane,W.S. and Reinberg,D.
  TITLE     Cloning of a human gene encoding the general transcription
            initiation factor IIB
  JOURNAL   Nature 352 (6337), 689-695 (1991)
   PUBMED   1876184
REFERENCE   10 (bases 1 to 1599)
  AUTHORS   Conaway,R.C. and Conaway,J.W.
  TITLE     ATP activates transcription initiation from promoters by RNA
            polymerase II in a reversible step prior to RNA synthesis
  JOURNAL   J Biol Chem 263 (6), 2962-2968 (1988)
   PUBMED   2449431
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL445991.15.
            
            On Nov 22, 2018 this sequence version replaced NM_001514.5.
            
            Summary: This gene encodes the general transcription factor IIB,
            one of the ubiquitous factors required for transcription initiation
            by RNA polymerase II. The protein localizes to the nucleus where it
            forms a complex (the DAB complex) with transcription factors IID
            and IIA. Transcription factor IIB serves as a bridge between IID,
            the factor which initially recognizes the promoter sequence, and
            RNA polymerase II. [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK289822.1, BC020597.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000370500.10/ ENSP00000359531.5
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-85                AL445991.15        54433-54517         c
            86-192              AL445991.15        50211-50317         c
            193-326             AL445991.15        26931-27064         c
            327-473             AL445991.15        23090-23236         c
            474-603             AL445991.15        22832-22961         c
            604-885             AL445991.15        20156-20437         c
            886-1599            AL445991.15        15583-16296         c
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p22.2"
     gene            1..1599
                     /gene="GTF2B"
                     /gene_synonym="TF2B; TFIIB"
                     /note="general transcription factor IIB"
                     /db_xref="GeneID:2959"
                     /db_xref="HGNC:HGNC:4648"
                     /db_xref="MIM:189963"
     exon            1..85
                     /gene="GTF2B"
                     /gene_synonym="TF2B; TFIIB"
                     /inference="alignment:Splign:2.1.0"
     CDS             69..1019
                     /gene="GTF2B"
                     /gene_synonym="TF2B; TFIIB"
                     /EC_number="2.3.1.48"
                     /note="RNA polymerase II transcription factor IIB;
                     S300-II; general transcription factor TFIIB"
                     /codon_start=1
                     /product="transcription initiation factor IIB"
                     /protein_id="NP_001505.1"
                     /db_xref="CCDS:CCDS715.1"
                     /db_xref="GeneID:2959"
                     /db_xref="HGNC:HGNC:4648"
                     /db_xref="MIM:189963"
                     /translation="MASTSRLDALPRVTCPNHPDAILVEDYRAGDMICPECGLVVGDR
                     VIDVGSEWRTFSNDKATKDPSRVGDSQNPLLSDGDLSTMIGKGTGAASFDEFGNSKYQ
                     NRRTMSSSDRAMMNAFKEITTMADRINLPRNIVDRTNNLFKQVYEQKSLKGRANDAIA
                     SACLYIACRQEGVPRTFKEICAVSRISKKEIGRCFKLILKALETSVDLITTGDFMSRF
                     CSNLCLPKQVQMAATHIARKAVELDLVPGRSPISVAAAAIYMASQASAEKRTQKEIGD
                     IAGVADVTIRQSYRLIYPRAPDLFPTDFKFDTPVDKLPQL"
     exon            86..192
                     /gene="GTF2B"
                     /gene_synonym="TF2B; TFIIB"
                     /inference="alignment:Splign:2.1.0"
     exon            193..326
                     /gene="GTF2B"
                     /gene_synonym="TF2B; TFIIB"
                     /inference="alignment:Splign:2.1.0"
     exon            327..473
                     /gene="GTF2B"
                     /gene_synonym="TF2B; TFIIB"
                     /inference="alignment:Splign:2.1.0"
     exon            474..603
                     /gene="GTF2B"
                     /gene_synonym="TF2B; TFIIB"
                     /inference="alignment:Splign:2.1.0"
     exon            604..885
                     /gene="GTF2B"
                     /gene_synonym="TF2B; TFIIB"
                     /inference="alignment:Splign:2.1.0"
     exon            886..1599
                     /gene="GTF2B"
                     /gene_synonym="TF2B; TFIIB"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1277..1282
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GTF2B"
                     /gene_synonym="TF2B; TFIIB"
                     /note="hexamer: AATAAA"
     polyA_site      1300
                     /gene="GTF2B"
                     /gene_synonym="TF2B; TFIIB"
                     /note="major polyA site"
     regulatory      1421..1426
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GTF2B"
                     /gene_synonym="TF2B; TFIIB"
                     /note="hexamer: AATAAA"
     polyA_site      1444
                     /gene="GTF2B"
                     /gene_synonym="TF2B; TFIIB"
     regulatory      1577..1582
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GTF2B"
                     /gene_synonym="TF2B; TFIIB"
                     /note="hexamer: ATTAAA"
     polyA_site      1583
                     /gene="GTF2B"
                     /gene_synonym="TF2B; TFIIB"
     polyA_site      1599
                     /gene="GTF2B"
                     /gene_synonym="TF2B; TFIIB"
ORIGIN      
        1 gccacattcg cttcctgctt tcggtgtgtc tgttgtgtct tgttgcgggc accgcagtcg
       61 ccgtgaagat ggcgtctacc agccgtttgg atgctcttcc aagagtcaca tgtccaaacc
      121 atccagatgc gattttagtg gaggactaca gagccggtga tatgatctgt cctgaatgtg
      181 gcttggttgt aggtgaccgg gttattgatg tgggatctga atggcgaact ttcagcaatg
      241 acaaagcaac aaaagatcca tctcgagttg gagattctca gaatcctctt ctgagtgatg
      301 gagatttgtc taccatgatt ggcaagggca caggagctgc aagttttgac gaatttggca
      361 attctaagta ccagaatcgg agaacaatga gcagttctga tcgggcaatg atgaatgcat
      421 tcaaagaaat cactaccatg gcagacagaa tcaatctacc tcgaaatata gttgatcgaa
      481 caaataattt attcaagcaa gtatatgaac agaagagcct gaagggaaga gctaatgatg
      541 ctatagcttc tgcttgtctc tatattgcct gtagacaaga aggggttcct aggacattta
      601 aagaaatatg tgccgtatca cgaatttcta agaaagaaat tggtcggtgt tttaaactta
      661 ttttgaaagc gctagaaacc agtgtggatt tgattacaac tggggacttc atgtccaggt
      721 tctgttccaa cctttgtctt cctaaacaag tacagatggc agctacacat atagcccgta
      781 aagctgtgga actggacttg gttcctggga ggagccccat ctctgtggca gcggcagcta
      841 tttacatggc ctcacaggca tcagctgaaa agaggaccca aaaagaaatt ggagatattg
      901 ctggtgttgc tgatgttaca atcagacagt cctatagact gatctatcct cgagccccag
      961 atctgtttcc tacagacttc aaatttgaca ccccagtgga caaactacca cagctataaa
     1021 ttgaggcagc taacgtcaaa ttcttgaata caaaactttg cctgttgtac atagcctata
     1081 caaaatgctg ggttgagcct ttcatgagga aaaacaaaag acatggtacg cattccaggg
     1141 ctgaatacta ttgcttggca ttctgtatgt atatactagt gaaacatatt taatgattta
     1201 aatttcttat caaatttctt ttgtagcaat ctaggaaact gtattttgga agatatttga
     1261 aattatgtaa ttcttgaata aaacattttt caaaactcaa gtttttgtta tatgttacat
     1321 gtaacttatg atacataatt acaaataatg caaatcattg cagctaataa agctgataga
     1381 ctttatttcc attacttata tatacatagt tttttatttt aataaattta tggaaagagc
     1441 aaaagctttt gagaaccatt gttaacatca acatcatagt ttccagtttg aaaggatgtg
     1501 tatgtgagat ttattatgta tattattaaa caagaagtga tgagcttggg ccttgaaagg
     1561 caccagcttg agagacatta aaatgttcta agtatgtga
//
