LOCUS       NM_004492               1559 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens general transcription factor IIA subunit 2 (GTF2A2),
            transcript variant 1, mRNA.
ACCESSION   NM_004492
VERSION     NM_004492.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   2  (bases 1 to 1559)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 1559)
  AUTHORS   Fragoza,R., Das,J., Wierbowski,S.D., Liang,J., Tran,T.N., Liang,S.,
            Beltran,J.F., Rivera-Erick,C.A., Ye,K., Wang,T.Y., Yao,L., Mort,M.,
            Stenson,P.D., Cooper,D.N., Wei,X., Keinan,A., Schimenti,J.C.,
            Clark,A.G. and Yu,H.
  TITLE     Extensive disruption of protein interactions by genetic variants
            across the allele frequency spectrum in human populations
  JOURNAL   Nat Commun 10 (1), 4141 (2019)
   PUBMED   31515488
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1559)
  AUTHORS   Wang,J., Zhao,S., He,W., Wei,Y., Zhang,Y., Pegg,H., Shore,P.,
            Roberts,S.G.E. and Deng,W.
  TITLE     A transcription factor IIA-binding site differentially regulates
            RNA polymerase II-mediated transcription in a promoter
            context-dependent manner
  JOURNAL   J Biol Chem 292 (28), 11873-11885 (2017)
   PUBMED   28539359
  REMARK    GeneRIF: Data suggest that many TATA-box-containing promoters
            contain a TFIIA recognition element (IIARE) that activates
            transcription by increasing the recruitment of RNA polymerase II,
            TFIIA (TF2A1/TF2A2 subunits), TAF4, and P300. (TAF4 = TATA box
            binding protein (TBP)-associated factor, RNA polymerase II, C1;
            P300 = E1A binding protein p300)
REFERENCE   5  (bases 1 to 1559)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Paulo,J.A., Cannon,J.R., Ting,L.,
            Baltier,K., Colby,G., Gebreab,F., Gygi,M.P., Parzen,H., Szpyt,J.,
            Tam,S., Zarraga,G., Pontano-Vaites,L., Swarup,S., White,A.E.,
            Schweppe,D.K., Rad,R., Erickson,B.K., Obar,R.A., Guruharsha,K.G.,
            Li,K., Artavanis-Tsakonas,S., Gygi,S.P. and Harper,J.W.
  TITLE     Architecture of the human interactome defines protein communities
            and disease networks
  JOURNAL   Nature 545 (7655), 505-509 (2017)
   PUBMED   28514442
REFERENCE   6  (bases 1 to 1559)
  AUTHORS   DeJong,J., Bernstein,R. and Roeder,R.G.
  TITLE     Human general transcription factor TFIIA: characterization of a
            cDNA encoding the small subunit and requirement for basal and
            activated transcription
  JOURNAL   Proc Natl Acad Sci U S A 92 (8), 3313-3317 (1995)
   PUBMED   7724559
REFERENCE   7  (bases 1 to 1559)
  AUTHORS   Sun,X., Ma,D., Sheldon,M., Yeung,K. and Reinberg,D.
  TITLE     Reconstitution of human TFIIA activity from recombinant
            polypeptides: a role in TFIID-mediated transcription
  JOURNAL   Genes Dev 8 (19), 2336-2348 (1994)
   PUBMED   7958900
REFERENCE   8  (bases 1 to 1559)
  AUTHORS   Ozer,J., Moore,P.A., Bolden,A.H., Lee,A., Rosen,C.A. and
            Lieberman,P.M.
  TITLE     Molecular cloning of the small (gamma) subunit of human TFIIA
            reveals functions critical for activated transcription
  JOURNAL   Genes Dev 8 (19), 2324-2335 (1994)
   PUBMED   7958899
REFERENCE   9  (bases 1 to 1559)
  AUTHORS   Jacob,G.A., Luse,S.W. and Luse,D.S.
  TITLE     Abortive initiation is increased only for the weakest members of a
            set of down mutants of the adenovirus 2 major late promoter
  JOURNAL   J Biol Chem 266 (33), 22537-22544 (1991)
   PUBMED   1939271
REFERENCE   10 (bases 1 to 1559)
  AUTHORS   Conaway,R.C. and Conaway,J.W.
  TITLE     ATP activates transcription initiation from promoters by RNA
            polymerase II in a reversible step prior to RNA synthesis
  JOURNAL   J Biol Chem 263 (6), 2962-2968 (1988)
   PUBMED   2449431
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK312015.1, BC000287.2, CV572751.1 and DB531003.1.
            
            On Nov 22, 2018 this sequence version replaced NM_004492.2.
            
            Summary: Accurate transcription initiation on TATA-containing class
            II genes involves the ordered assembly of RNA polymerase II
            (POLR2A; MIM 180660) and the general initiation factors TFIIA,
            TFIIB (MIM 189963), TFIID (MIM 313650), TFIIE (MIM 189962), TFIIF
            (MIM 189968), TFIIG/TFIIJ, and TFIIH (MIM 189972). The first step
            involves recognition of the TATA element by the TATA-binding
            subunit (TBP; MIM 600075) and may be regulated by TFIIA, a factor
            that interacts with both TBP and a TBP-associated factor (TAF; MIM
            600475) in TFIID. TFIIA has 2 subunits (43 and 12 kD) in yeast and
            3 subunits in higher eukaryotes. In HeLa extracts, it consists of a
            35-kD alpha subunit and a 19-kD beta subunit encoded by the N- and
            C-terminal regions of GTF2A1 (MIM 600520), respectively, and a
            12-kD gamma subunit encoded by GTF2A2 (DeJong et al., 1995 [PubMed
            7724559]).[supplied by OMIM, Mar 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR5189655.73923.1,
                                           SRR5189658.92639.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN03267760, SAMN03267776
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000396060.7/ ENSP00000379372.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-390               AK312015.1         24-413
            391-755             BC000287.2         376-740
            756-1175            CV572751.1         177-596
            1176-1559           DB531003.1         15-398
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="15"
                     /map="15q22.2"
     gene            1..1559
                     /gene="GTF2A2"
                     /gene_synonym="HsT18745; T18745; TF2A2; TFIIA; TFIIA-12;
                     TFIIA-gamma; TFIIAS"
                     /note="general transcription factor IIA subunit 2"
                     /db_xref="GeneID:2958"
                     /db_xref="HGNC:HGNC:4647"
                     /db_xref="MIM:600519"
     exon            1..110
                     /gene="GTF2A2"
                     /gene_synonym="HsT18745; T18745; TF2A2; TFIIA; TFIIA-12;
                     TFIIA-gamma; TFIIAS"
                     /inference="alignment:Splign:2.1.0"
     exon            111..231
                     /gene="GTF2A2"
                     /gene_synonym="HsT18745; T18745; TF2A2; TFIIA; TFIIA-12;
                     TFIIA-gamma; TFIIAS"
                     /inference="alignment:Splign:2.1.0"
     CDS             160..489
                     /gene="GTF2A2"
                     /gene_synonym="HsT18745; T18745; TF2A2; TFIIA; TFIIA-12;
                     TFIIA-gamma; TFIIAS"
                     /note="TFIIA p12 subunit; transcription initiation factor
                     IIA gamma chain; general transcription factor IIA, 2,
                     12kDa; TFIIA gamma subunit; general transcription factor
                     IIA 2"
                     /codon_start=1
                     /product="transcription initiation factor IIA subunit 2"
                     /protein_id="NP_004483.1"
                     /db_xref="CCDS:CCDS10173.1"
                     /db_xref="GeneID:2958"
                     /db_xref="HGNC:HGNC:4647"
                     /db_xref="MIM:600519"
                     /translation="MAYQLYRNTTLGNSLQESLDELIQSQQITPQLALQVLLQFDKAI
                     NAALAQRVRNRVNFRGSLNTYRFCDNVWTFVLNDVEFREVTELIKVDKVKIVACDGKN
                     TGSNTTE"
     exon            232..336
                     /gene="GTF2A2"
                     /gene_synonym="HsT18745; T18745; TF2A2; TFIIA; TFIIA-12;
                     TFIIA-gamma; TFIIAS"
                     /inference="alignment:Splign:2.1.0"
     exon            337..463
                     /gene="GTF2A2"
                     /gene_synonym="HsT18745; T18745; TF2A2; TFIIA; TFIIA-12;
                     TFIIA-gamma; TFIIAS"
                     /inference="alignment:Splign:2.1.0"
     exon            464..1559
                     /gene="GTF2A2"
                     /gene_synonym="HsT18745; T18745; TF2A2; TFIIA; TFIIA-12;
                     TFIIA-gamma; TFIIAS"
                     /inference="alignment:Splign:2.1.0"
     regulatory      732..737
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GTF2A2"
                     /gene_synonym="HsT18745; T18745; TF2A2; TFIIA; TFIIA-12;
                     TFIIA-gamma; TFIIAS"
                     /note="hexamer: AATAAA"
     polyA_site      755
                     /gene="GTF2A2"
                     /gene_synonym="HsT18745; T18745; TF2A2; TFIIA; TFIIA-12;
                     TFIIA-gamma; TFIIAS"
                     /note="major polyA site"
     regulatory      861..866
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GTF2A2"
                     /gene_synonym="HsT18745; T18745; TF2A2; TFIIA; TFIIA-12;
                     TFIIA-gamma; TFIIAS"
                     /note="hexamer: AATAAA"
     polyA_site      887
                     /gene="GTF2A2"
                     /gene_synonym="HsT18745; T18745; TF2A2; TFIIA; TFIIA-12;
                     TFIIA-gamma; TFIIAS"
     regulatory      1537..1542
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GTF2A2"
                     /gene_synonym="HsT18745; T18745; TF2A2; TFIIA; TFIIA-12;
                     TFIIA-gamma; TFIIAS"
                     /note="hexamer: AATAAA"
     polyA_site      1559
                     /gene="GTF2A2"
                     /gene_synonym="HsT18745; T18745; TF2A2; TFIIA; TFIIA-12;
                     TFIIA-gamma; TFIIAS"
ORIGIN      
        1 agttctgcgg tttctgcggc ggctggagag gtggtcggag aagtaggaac ctcctgccgg
       61 gctcgtggcg gcttctgtcc gctccgcgga gggaagcgcc ttccccacag gacatcaatg
      121 caagcttgaa taagaaaaac aaattcttcc tcctaagcca tggcatatca gttatacaga
      181 aatactactt tgggaaacag tcttcaggag agcctagatg agctcataca gtctcaacag
      241 atcacccccc aacttgccct tcaagttcta cttcagtttg ataaggctat aaatgcagca
      301 ctggctcaga gggtcaggaa cagagtcaat ttcaggggct ctctaaatac gtacagattc
      361 tgcgataatg tgtggacttt tgtactgaat gatgttgaat tcagagaggt gacagaactt
      421 attaaagtgg ataaagtgaa aattgtagcc tgtgatggta aaaatactgg ctccaatact
      481 acagaatgaa tagaaaaaat atgacttttt tacaccatct tctgttattc attgcttttg
      541 aagagaagca tagaagagac tttttattta ttctagaatt gcagaaatga ctacactgtg
      601 ctataccaga gaattccagt agaaagaaac ttgtaactct gtagcctctt acatcacctt
      661 tattatacag catgaaaaac cataactttt ttttaaggac aaaagttgtt gccttcctaa
      721 gaaccttctt taataaactc attttaaaac tctgagtaat aactggtgaa gctattaagg
      781 tagcaatatg aagatattag ataatgcatt ttcccatttc ctttgcaagg aacatgatgc
      841 cctttcagaa tgcttatttc aataaagtca tgttttgtca gttgtaactt tacctgatga
      901 aagcatcagc aggggcaaaa ccaaactctg gatggaattt tttaagaaaa tcccttgctt
      961 tttaaaatac aaaaccaaag ttaatcattg gtataaaaaa acccaagtac aacttgatta
     1021 ggaaaagtta gacaatcata gcaaaaacaa aaccaaaaaa ctttccttct tagaccaggt
     1081 aacatgagga agggggaaaa aaacccaaac aaaatagtcc ccccacatga caggtgtctt
     1141 cagcttgcca tattgatcca aagttcatac attttatcat gtacaagata cattagactt
     1201 acaataaagt ctaattatta gacttttatg tcagtggatt gtgcttgcag aaatacagaa
     1261 gtaacatact gaccctaggt gaggagtttc ttacatggtt aactggattg acccaattgg
     1321 agtaaaagat ttttgctgtt acctcgatgc tcctgcagtt gttacctcag catgccactg
     1381 tagtccttga ctctcacatt aaaactaact ttggctaggt actggtggct cacacctgta
     1441 agtaatccca gtactttagg aggctgagac tggaggatta cttgagtcca ggagttggag
     1501 accagcctgg ccaatataga ctgtccctac aaagaaaata aaattgtttc atcttccaa
//
