LOCUS       NM_001320929            1631 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens general transcription factor IIA subunit 2 (GTF2A2),
            transcript variant 2, mRNA.
ACCESSION   NM_001320929 XM_005254325
VERSION     NM_001320929.2
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1631)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   2  (bases 1 to 1631)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 1631)
  AUTHORS   Fragoza,R., Das,J., Wierbowski,S.D., Liang,J., Tran,T.N., Liang,S.,
            Beltran,J.F., Rivera-Erick,C.A., Ye,K., Wang,T.Y., Yao,L., Mort,M.,
            Stenson,P.D., Cooper,D.N., Wei,X., Keinan,A., Schimenti,J.C.,
            Clark,A.G. and Yu,H.
  TITLE     Extensive disruption of protein interactions by genetic variants
            across the allele frequency spectrum in human populations
  JOURNAL   Nat Commun 10 (1), 4141 (2019)
   PUBMED   31515488
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1631)
  AUTHORS   Wang,J., Zhao,S., He,W., Wei,Y., Zhang,Y., Pegg,H., Shore,P.,
            Roberts,S.G.E. and Deng,W.
  TITLE     A transcription factor IIA-binding site differentially regulates
            RNA polymerase II-mediated transcription in a promoter
            context-dependent manner
  JOURNAL   J Biol Chem 292 (28), 11873-11885 (2017)
   PUBMED   28539359
  REMARK    GeneRIF: Data suggest that many TATA-box-containing promoters
            contain a TFIIA recognition element (IIARE) that activates
            transcription by increasing the recruitment of RNA polymerase II,
            TFIIA (TF2A1/TF2A2 subunits), TAF4, and P300. (TAF4 = TATA box
            binding protein (TBP)-associated factor, RNA polymerase II, C1;
            P300 = E1A binding protein p300)
REFERENCE   5  (bases 1 to 1631)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Paulo,J.A., Cannon,J.R., Ting,L.,
            Baltier,K., Colby,G., Gebreab,F., Gygi,M.P., Parzen,H., Szpyt,J.,
            Tam,S., Zarraga,G., Pontano-Vaites,L., Swarup,S., White,A.E.,
            Schweppe,D.K., Rad,R., Erickson,B.K., Obar,R.A., Guruharsha,K.G.,
            Li,K., Artavanis-Tsakonas,S., Gygi,S.P. and Harper,J.W.
  TITLE     Architecture of the human interactome defines protein communities
            and disease networks
  JOURNAL   Nature 545 (7655), 505-509 (2017)
   PUBMED   28514442
REFERENCE   6  (bases 1 to 1631)
  AUTHORS   DeJong,J., Bernstein,R. and Roeder,R.G.
  TITLE     Human general transcription factor TFIIA: characterization of a
            cDNA encoding the small subunit and requirement for basal and
            activated transcription
  JOURNAL   Proc Natl Acad Sci U S A 92 (8), 3313-3317 (1995)
   PUBMED   7724559
REFERENCE   7  (bases 1 to 1631)
  AUTHORS   Sun,X., Ma,D., Sheldon,M., Yeung,K. and Reinberg,D.
  TITLE     Reconstitution of human TFIIA activity from recombinant
            polypeptides: a role in TFIID-mediated transcription
  JOURNAL   Genes Dev 8 (19), 2336-2348 (1994)
   PUBMED   7958900
REFERENCE   8  (bases 1 to 1631)
  AUTHORS   Ozer,J., Moore,P.A., Bolden,A.H., Lee,A., Rosen,C.A. and
            Lieberman,P.M.
  TITLE     Molecular cloning of the small (gamma) subunit of human TFIIA
            reveals functions critical for activated transcription
  JOURNAL   Genes Dev 8 (19), 2324-2335 (1994)
   PUBMED   7958899
REFERENCE   9  (bases 1 to 1631)
  AUTHORS   Jacob,G.A., Luse,S.W. and Luse,D.S.
  TITLE     Abortive initiation is increased only for the weakest members of a
            set of down mutants of the adenovirus 2 major late promoter
  JOURNAL   J Biol Chem 266 (33), 22537-22544 (1991)
   PUBMED   1939271
REFERENCE   10 (bases 1 to 1631)
  AUTHORS   Conaway,R.C. and Conaway,J.W.
  TITLE     ATP activates transcription initiation from promoters by RNA
            polymerase II in a reversible step prior to RNA synthesis
  JOURNAL   J Biol Chem 263 (6), 2962-2968 (1988)
   PUBMED   2449431
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC092755.4, HY036972.1, HY002503.1 and BX099306.1.
            
            On May 31, 2019 this sequence version replaced NM_001320929.1.
            
            Summary: Accurate transcription initiation on TATA-containing class
            II genes involves the ordered assembly of RNA polymerase II
            (POLR2A; MIM 180660) and the general initiation factors TFIIA,
            TFIIB (MIM 189963), TFIID (MIM 313650), TFIIE (MIM 189962), TFIIF
            (MIM 189968), TFIIG/TFIIJ, and TFIIH (MIM 189972). The first step
            involves recognition of the TATA element by the TATA-binding
            subunit (TBP; MIM 600075) and may be regulated by TFIIA, a factor
            that interacts with both TBP and a TBP-associated factor (TAF; MIM
            600475) in TFIID. TFIIA has 2 subunits (43 and 12 kD) in yeast and
            3 subunits in higher eukaryotes. In HeLa extracts, it consists of a
            35-kD alpha subunit and a 19-kD beta subunit encoded by the N- and
            C-terminal regions of GTF2A1 (MIM 600520), respectively, and a
            12-kD gamma subunit encoded by GTF2A2 (DeJong et al., 1995 [PubMed
            7724559]).[supplied by OMIM, Mar 2008].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: HY002503.1, HY036972.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-51                AC092755.4         79289-79339         c
            52-84               HY036972.1         1-33
            85-155              AC092755.4         79185-79255         c
            156-627             HY002503.1         92-563
            628-1542            AC092755.4         60185-61099         c
            1543-1631           BX099306.1         534-622
FEATURES             Location/Qualifiers
     source          1..1631
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="15"
                     /map="15q22.2"
     gene            1..1631
                     /gene="GTF2A2"
                     /gene_synonym="HsT18745; T18745; TF2A2; TFIIA; TFIIA-12;
                     TFIIA-gamma; TFIIAS"
                     /note="general transcription factor IIA subunit 2"
                     /db_xref="GeneID:2958"
                     /db_xref="HGNC:HGNC:4647"
                     /db_xref="MIM:600519"
     exon            1..182
                     /gene="GTF2A2"
                     /gene_synonym="HsT18745; T18745; TF2A2; TFIIA; TFIIA-12;
                     TFIIA-gamma; TFIIAS"
                     /inference="alignment:Splign:2.1.0"
     exon            183..303
                     /gene="GTF2A2"
                     /gene_synonym="HsT18745; T18745; TF2A2; TFIIA; TFIIA-12;
                     TFIIA-gamma; TFIIAS"
                     /inference="alignment:Splign:2.1.0"
     CDS             232..561
                     /gene="GTF2A2"
                     /gene_synonym="HsT18745; T18745; TF2A2; TFIIA; TFIIA-12;
                     TFIIA-gamma; TFIIAS"
                     /note="TFIIA p12 subunit; transcription initiation factor
                     IIA gamma chain; general transcription factor IIA, 2,
                     12kDa; TFIIA gamma subunit; general transcription factor
                     IIA 2"
                     /codon_start=1
                     /product="transcription initiation factor IIA subunit 2"
                     /protein_id="NP_001307858.1"
                     /db_xref="CCDS:CCDS10173.1"
                     /db_xref="GeneID:2958"
                     /db_xref="HGNC:HGNC:4647"
                     /db_xref="MIM:600519"
                     /translation="MAYQLYRNTTLGNSLQESLDELIQSQQITPQLALQVLLQFDKAI
                     NAALAQRVRNRVNFRGSLNTYRFCDNVWTFVLNDVEFREVTELIKVDKVKIVACDGKN
                     TGSNTTE"
     exon            304..408
                     /gene="GTF2A2"
                     /gene_synonym="HsT18745; T18745; TF2A2; TFIIA; TFIIA-12;
                     TFIIA-gamma; TFIIAS"
                     /inference="alignment:Splign:2.1.0"
     exon            409..535
                     /gene="GTF2A2"
                     /gene_synonym="HsT18745; T18745; TF2A2; TFIIA; TFIIA-12;
                     TFIIA-gamma; TFIIAS"
                     /inference="alignment:Splign:2.1.0"
     exon            536..1631
                     /gene="GTF2A2"
                     /gene_synonym="HsT18745; T18745; TF2A2; TFIIA; TFIIA-12;
                     TFIIA-gamma; TFIIAS"
                     /inference="alignment:Splign:2.1.0"
     regulatory      804..809
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GTF2A2"
                     /gene_synonym="HsT18745; T18745; TF2A2; TFIIA; TFIIA-12;
                     TFIIA-gamma; TFIIAS"
                     /note="hexamer: AATAAA"
     polyA_site      827
                     /gene="GTF2A2"
                     /gene_synonym="HsT18745; T18745; TF2A2; TFIIA; TFIIA-12;
                     TFIIA-gamma; TFIIAS"
                     /note="major polyA site"
     regulatory      933..938
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GTF2A2"
                     /gene_synonym="HsT18745; T18745; TF2A2; TFIIA; TFIIA-12;
                     TFIIA-gamma; TFIIAS"
                     /note="hexamer: AATAAA"
     polyA_site      959
                     /gene="GTF2A2"
                     /gene_synonym="HsT18745; T18745; TF2A2; TFIIA; TFIIA-12;
                     TFIIA-gamma; TFIIAS"
     regulatory      1609..1614
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GTF2A2"
                     /gene_synonym="HsT18745; T18745; TF2A2; TFIIA; TFIIA-12;
                     TFIIA-gamma; TFIIAS"
                     /note="hexamer: AATAAA"
     polyA_site      1631
                     /gene="GTF2A2"
                     /gene_synonym="HsT18745; T18745; TF2A2; TFIIA; TFIIA-12;
                     TFIIA-gamma; TFIIAS"
ORIGIN      
        1 ccttcgctcc tgggatctgc ccgttaccga ccgttgccgg caaggccccg cggccggcac
       61 cggagaaggg cggcgaggcg gcggtgatgg tcgcgtcccg tggcgggagg ctcgtcgttc
      121 acggggcgcc cacagaaccc acctagggga ggtcttcagc ccaccaccgt attttcagcc
      181 aggacatcaa tgcaagcttg aataagaaaa acaaattctt cctcctaagc catggcatat
      241 cagttataca gaaatactac tttgggaaac agtcttcagg agagcctaga tgagctcata
      301 cagtctcaac agatcacccc ccaacttgcc cttcaagttc tacttcagtt tgataaggct
      361 ataaatgcag cactggctca gagggtcagg aacagagtca atttcagggg ctctctaaat
      421 acgtacagat tctgcgataa tgtgtggact tttgtactga atgatgttga attcagagag
      481 gtgacagaac ttattaaagt ggataaagtg aaaattgtag cctgtgatgg taaaaatact
      541 ggctccaata ctacagaatg aatagaaaaa atatgacttt tttacaccat cttctgttat
      601 tcattgcttt tgaagagaag catagaagag actttttatt tattctagaa ttgcagaaat
      661 gactacactg tgctatacca gagaattcca gtagaaagaa acttgtaact ctgtagcctc
      721 ttacatcacc tttattatac agcatgaaaa accataactt ttttttaagg acaaaagttg
      781 ttgccttcct aagaaccttc tttaataaac tcattttaaa actctgagta ataactggtg
      841 aagctattaa ggtagcaata tgaagatatt agataatgca ttttcccatt tcctttgcaa
      901 ggaacatgat gccctttcag aatgcttatt tcaataaagt catgttttgt cagttgtaac
      961 tttacctgat gaaagcatca gcaggggcaa aaccaaactc tggatggaat tttttaagaa
     1021 aatcccttgc tttttaaaat acaaaaccaa agttaatcat tggtataaaa aaacccaagt
     1081 acaacttgat taggaaaagt tagacaatca tagcaaaaac aaaaccaaaa aactttcctt
     1141 cttagaccag gtaacatgag gaagggggaa aaaaacccaa acaaaatagt ccccccacat
     1201 gacaggtgtc ttcagcttgc catattgatc caaagttcat acattttatc atgtacaaga
     1261 tacattagac ttacaataaa gtctaattat tagactttta tgtcagtgga ttgtgcttgc
     1321 agaaatacag aagtaacata ctgaccctag gtgaggagtt tcttacatgg ttaactggat
     1381 tgacccaatt ggagtaaaag atttttgctg ttacctcgat gctcctgcag ttgttacctc
     1441 agcatgccac tgtagtcctt gactctcaca ttaaaactaa ctttggctag gtactggtgg
     1501 ctcacacctg taagtaatcc cagtacttta ggaggctgag actggaggat tacttgagtc
     1561 caggagttgg agaccagcct ggccaatata gactgtccct acaaagaaaa taaaattgtt
     1621 tcatcttcca a
//
