LOCUS       NM_002094               7141 bp    mRNA    linear   PRI 24-SEP-2024
DEFINITION  Homo sapiens G1 to S phase transition 1 (GSPT1), transcript variant
            1, mRNA.
ACCESSION   NM_002094
VERSION     NM_002094.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7141)
  AUTHORS   Sasayama,T., Hamada,T., Tanaka,K., Nagashima,H., Yamanishi,S. and
            Ueyama,T.
  TITLE     Potential of GSPT1 as a novel target for glioblastoma therapy
  JOURNAL   Cell Death Dis 15 (8), 572 (2024)
   PUBMED   39117611
  REMARK    GeneRIF: Potential of GSPT1 as a novel target for glioblastoma
            therapy.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7141)
  AUTHORS   Chen,X., Feng,C., Zha,J., Shen,Z. and Ji,W.
  TITLE     MicroRNA-508-3p regulates the proliferation of human lung cancer
            cells by targeting G1 to S phase transition 1 (GSPT1) protein
  JOURNAL   Acta Biochim Pol 70 (4), 835-841 (2023)
   PUBMED   38099479
  REMARK    GeneRIF: MicroRNA-508-3p regulates the proliferation of human lung
            cancer cells by targeting G1 to S phase transition 1 (GSPT1)
            protein.
REFERENCE   3  (bases 1 to 7141)
  AUTHORS   Xi,Y.Q., Gao,J.B., Li,X.F., Xu,L.H., Li,Z., Yang,L.J., Wang,J.,
            Wang,H.Q., Fang,X.C., Huang,S.R., Xie,W., Feng,M.H. and Zhang,J.W.
  TITLE     GSPT1 Functions as a Tumor Promoter in Human Liver Cancer
  JOURNAL   Curr Med Sci 43 (1), 104-114 (2023)
   PUBMED   36459303
  REMARK    GeneRIF: GSPT1 Functions as a Tumor Promoter in Human Liver Cancer.
REFERENCE   4  (bases 1 to 7141)
  AUTHORS   Hu,F., Peng,Y., Chang,S., Luo,X., Yuan,Y., Zhu,X., Xu,Y., Du,K.,
            Chen,Y., Deng,S., Yu,F., Feng,X., Fan,X., Ashktorab,H., Smoot,D.,
            Meltzer,S.J., Li,S., Wei,Y., Zhang,X. and Jin,Z.
  TITLE     Vimentin binds to a novel tumor suppressor protein, GSPT1-238aa,
            encoded by circGSPT1 with a selective encoding priority to halt
            autophagy in gastric carcinoma
  JOURNAL   Cancer Lett 545, 215826 (2022)
   PUBMED   35839920
  REMARK    GeneRIF: Vimentin binds to a novel tumor suppressor protein,
            GSPT1-238aa, encoded by circGSPT1 with a selective encoding
            priority to halt autophagy in gastric carcinoma.
REFERENCE   5  (bases 1 to 7141)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   6  (bases 1 to 7141)
  AUTHORS   Hoshino,S., Imai,M., Mizutani,M., Kikuchi,Y., Hanaoka,F., Ui,M. and
            Katada,T.
  TITLE     Molecular cloning of a novel member of the eukaryotic polypeptide
            chain-releasing factors (eRF). Its identification as eRF3
            interacting with eRF1
  JOURNAL   J Biol Chem 273 (35), 22254-22259 (1998)
   PUBMED   9712840
REFERENCE   7  (bases 1 to 7141)
  AUTHORS   Czaplinski,K., Ruiz-Echevarria,M.J., Paushkin,S.V., Han,X.,
            Weng,Y., Perlick,H.A., Dietz,H.C., Ter-Avanesyan,M.D. and
            Peltz,S.W.
  TITLE     The surveillance complex interacts with the translation release
            factors to enhance termination and degrade aberrant mRNAs
  JOURNAL   Genes Dev 12 (11), 1665-1677 (1998)
   PUBMED   9620853
REFERENCE   8  (bases 1 to 7141)
  AUTHORS   Ozawa,K., Murakami,Y., Eki,T., Yokoyama,K., Soeda,E., Hoshino,S.,
            Ui,M. and Hanaoka,F.
  TITLE     Mapping of the human GSPT1 gene, a human homolog of the yeast GST1
            gene, to chromosomal band 16p13.1
  JOURNAL   Somat Cell Mol Genet 18 (2), 189-194 (1992)
   PUBMED   1574740
REFERENCE   9  (bases 1 to 7141)
  AUTHORS   Hoshino,S., Miyazawa,H., Enomoto,T., Hanaoka,F., Kikuchi,Y.,
            Kikuchi,A. and Ui,M.
  TITLE     A human homologue of the yeast GST1 gene codes for a GTP-binding
            protein and is expressed in a proliferation-dependent manner in
            mammalian cells
  JOURNAL   EMBO J 8 (12), 3807-3814 (1989)
   PUBMED   2511002
REFERENCE   10 (bases 1 to 7141)
  AUTHORS   Kikuchi,Y., Shimatake,H. and Kikuchi,A.
  TITLE     A yeast gene required for the G1-to-S transition encodes a protein
            containing an A-kinase target site and GTPase domain
  JOURNAL   EMBO J 7 (4), 1175-1182 (1988)
   PUBMED   2841115
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC007216.2.
            
            On Nov 23, 2018 this sequence version replaced NM_002094.3.
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The extent of this transcript is
            supported by transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803612.43615.1,
                                           SRR1660805.27369.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000434724.7/ ENSP00000398131.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-588               AC007216.2         111735-112322
            589-630             AC007216.2         129656-129697
            631-672             AC007216.2         129810-129851
            673-900             AC007216.2         130906-131133
            901-934             AC007216.2         132704-132737
            935-1012            AC007216.2         136552-136629
            1013-1193           AC007216.2         139941-140121
            1194-1348           AC007216.2         140760-140914
            1349-1489           AC007216.2         141080-141220
            1490-1583           AC007216.2         142417-142510
            1584-1664           AC007216.2         144596-144676
            1665-1838           AC007216.2         150111-150284
            1839-1928           AC007216.2         151516-151605
            1929-2097           AC007216.2         151762-151930
            2098-7141           AC007216.2         154520-159563
FEATURES             Location/Qualifiers
     source          1..7141
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="16"
                     /map="16p13.13"
     gene            1..7141
                     /gene="GSPT1"
                     /gene_synonym="551G9.2; eRF3a; ETF3A; GST1"
                     /note="G1 to S phase transition 1"
                     /db_xref="GeneID:2935"
                     /db_xref="HGNC:HGNC:4621"
                     /db_xref="MIM:139259"
     exon            1..588
                     /gene="GSPT1"
                     /gene_synonym="551G9.2; eRF3a; ETF3A; GST1"
                     /inference="alignment:Splign:2.1.0"
     CDS             237..2150
                     /gene="GSPT1"
                     /gene_synonym="551G9.2; eRF3a; ETF3A; GST1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     eukaryotic peptide chain release factor GTP-binding
                     subunit ERF3A; G1 to S phase transition protein 1 homolog;
                     eukaryotic peptide chain release factor subunit 3a;
                     eukaryotic release factor 3a"
                     /codon_start=1
                     /product="eukaryotic peptide chain release factor
                     GTP-binding subunit ERF3A isoform 1"
                     /protein_id="NP_002085.3"
                     /db_xref="CCDS:CCDS45412.1"
                     /db_xref="GeneID:2935"
                     /db_xref="HGNC:HGNC:4621"
                     /db_xref="MIM:139259"
                     /translation="MDPGSGGGGGGGGGGGSSSGSSSSDSAPDCWDQADMEAPGPGPC
                     GGGGSLAAAAEAQRENLSAAFSRQLNVNAKPFVPNVHAAEFVPSFLRGPAAPPPPVGG
                     AANNHGAGSGAGGRAAPVESSQEEQSLCEGSNSAVSMELSEPIVENGETEMSPEESWE
                     HKEEISEAEPGGGSLGDGRPPEESAHEMMEEEEEIPKPKSVVAPPGAPKKEHVNVVFI
                     GHVDAGKSTIGGQIMYLTGMVDKRTLEKYEREAKEKNRETWYLSWALDTNQEERDKGK
                     TVEVGRAYFETEKKHFTILDAPGHKSFVPNMIGGASQADLAVLVISARKGEFETGFEK
                     GGQTREHAMLAKTAGVKHLIVLINKMDDPTVNWSNERYEECKEKLVPFLKKVGFNPKK
                     DIHFMPCSGLTGANLKEQSDFCPWYIGLPFIPYLDNLPNFNRSVDGPIRLPIVDKYKD
                     MGTVVLGKLESGSICKGQQLVMMPNKHNVEVLGILSDDVETDTVAPGENLKIRLKGIE
                     EEEILPGFILCDPNNLCHSGRTFDAQIVIIEHKSIICPGYNAVLHIHTCIEEVEITAL
                     ICLVDKKSGEKSKTRPRFVKQDQVCIARLRTAGTICLETFKDFPQMGRFTLRDEGKTI
                     AIGKVLKLVPEKD"
     exon            589..630
                     /gene="GSPT1"
                     /gene_synonym="551G9.2; eRF3a; ETF3A; GST1"
                     /inference="alignment:Splign:2.1.0"
     exon            631..672
                     /gene="GSPT1"
                     /gene_synonym="551G9.2; eRF3a; ETF3A; GST1"
                     /inference="alignment:Splign:2.1.0"
     exon            673..900
                     /gene="GSPT1"
                     /gene_synonym="551G9.2; eRF3a; ETF3A; GST1"
                     /inference="alignment:Splign:2.1.0"
     exon            901..934
                     /gene="GSPT1"
                     /gene_synonym="551G9.2; eRF3a; ETF3A; GST1"
                     /inference="alignment:Splign:2.1.0"
     exon            935..1012
                     /gene="GSPT1"
                     /gene_synonym="551G9.2; eRF3a; ETF3A; GST1"
                     /inference="alignment:Splign:2.1.0"
     exon            1013..1193
                     /gene="GSPT1"
                     /gene_synonym="551G9.2; eRF3a; ETF3A; GST1"
                     /inference="alignment:Splign:2.1.0"
     exon            1194..1348
                     /gene="GSPT1"
                     /gene_synonym="551G9.2; eRF3a; ETF3A; GST1"
                     /inference="alignment:Splign:2.1.0"
     exon            1349..1489
                     /gene="GSPT1"
                     /gene_synonym="551G9.2; eRF3a; ETF3A; GST1"
                     /inference="alignment:Splign:2.1.0"
     exon            1490..1583
                     /gene="GSPT1"
                     /gene_synonym="551G9.2; eRF3a; ETF3A; GST1"
                     /inference="alignment:Splign:2.1.0"
     exon            1584..1664
                     /gene="GSPT1"
                     /gene_synonym="551G9.2; eRF3a; ETF3A; GST1"
                     /inference="alignment:Splign:2.1.0"
     exon            1665..1838
                     /gene="GSPT1"
                     /gene_synonym="551G9.2; eRF3a; ETF3A; GST1"
                     /inference="alignment:Splign:2.1.0"
     exon            1839..1928
                     /gene="GSPT1"
                     /gene_synonym="551G9.2; eRF3a; ETF3A; GST1"
                     /inference="alignment:Splign:2.1.0"
     exon            1929..2097
                     /gene="GSPT1"
                     /gene_synonym="551G9.2; eRF3a; ETF3A; GST1"
                     /inference="alignment:Splign:2.1.0"
     exon            2098..7141
                     /gene="GSPT1"
                     /gene_synonym="551G9.2; eRF3a; ETF3A; GST1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2570..2575
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GSPT1"
                     /gene_synonym="551G9.2; eRF3a; ETF3A; GST1"
                     /note="hexamer: AATAAA"
     polyA_site      2590
                     /gene="GSPT1"
                     /gene_synonym="551G9.2; eRF3a; ETF3A; GST1"
                     /note="major polyA site"
     regulatory      3039..3044
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GSPT1"
                     /gene_synonym="551G9.2; eRF3a; ETF3A; GST1"
                     /note="hexamer: AATAAA"
     polyA_site      3067
                     /gene="GSPT1"
                     /gene_synonym="551G9.2; eRF3a; ETF3A; GST1"
     regulatory      7114..7119
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GSPT1"
                     /gene_synonym="551G9.2; eRF3a; ETF3A; GST1"
                     /note="hexamer: AATAAA"
     polyA_site      7141
                     /gene="GSPT1"
                     /gene_synonym="551G9.2; eRF3a; ETF3A; GST1"
                     /note="major polyA site"
ORIGIN      
        1 gctcacacac acgaggagga gggttgagct gccgccgccg ccgcctctgt cgtcgtcgcg
       61 agtgtggagt cgggactgga gctgctgccg cggcgacgcc ggggatcttt gtcgctagct
      121 cccggccctt ctgccccgcc gccttccctc agtcagcgtt gcccactcct ctccggccgg
      181 gcgcccctgc ctccatttcc cgctctctgt ccaccacaca cacggccccc ccgatcatgg
      241 atccgggcag tggcggcggc ggcggcggcg gcggcggcgg cgggagcagc agcggcagca
      301 gcagcagcga ctcggcgcct gactgctggg accaggcgga catggaagcc cccgggccgg
      361 gcccttgcgg cggcggcggc tccctggcgg cggcggccga ggcccagcgg gagaacctca
      421 gcgcggcctt cagccggcaa ctcaacgtca acgccaagcc cttcgtgccc aacgtccacg
      481 ccgccgagtt cgtgccgtcc ttcctgcggg gcccggcagc gccgccaccc ccagttggcg
      541 gcgccgccaa taaccacgga gccggcagcg gcgcgggagg ccgtgcggca cctgtggaat
      601 cctctcaaga ggaacagtca ttgtgtgaag gttcaaattc agctgttagc atggaacttt
      661 cagaacctat tgtagaaaat ggagagacag aaatgtctcc agaagaatca tgggagcaca
      721 aagaagaaat aagtgaagca gagccagggg gtggttcctt gggagatgga aggccgccag
      781 aggaaagtgc ccatgaaatg atggaggagg aagaggaaat cccaaaacct aagtctgtgg
      841 ttgcaccgcc aggtgctcct aagaaagagc atgtaaatgt agtattcatt gggcacgtag
      901 atgctggcaa gtcaaccatt ggaggacaaa taatgtattt gactggaatg gttgacaaaa
      961 ggacgcttga aaagtatgaa agagaagcta aagagaaaaa cagagaaact tggtacttgt
     1021 cttgggcctt agacacaaat caggaagaac gagacaaggg taaaacagta gaagtgggtc
     1081 gtgcctattt tgaaaccgaa aagaagcatt tcacaattct agatgcccct ggccacaaga
     1141 gttttgtccc aaatatgatt ggtggtgcct ctcaagctga tttggctgtg ctggtaatct
     1201 cagccaggaa aggagagttt gaaactggat ttgaaaaagg aggacagaca agagaacatg
     1261 caatgttggc aaagacagca ggtgtaaaac acctaattgt gctaattaat aagatggatg
     1321 atccaacagt aaattggagc aatgagagat atgaagaatg taaggagaaa ctagtgccat
     1381 ttttgaaaaa agttggcttc aatcccaaaa aggacattca ctttatgccc tgctcaggac
     1441 ttactggagc aaatctcaaa gagcagtcgg atttctgtcc ttggtacatt ggattaccgt
     1501 ttattccata tctggataat ttgccgaact tcaatagatc agttgatgga ccaatcaggc
     1561 tgccaattgt ggataagtac aaggatatgg gcactgtggt cctgggaaag ctggaatcag
     1621 gatctatttg taaaggccag cagcttgtga tgatgccaaa caagcacaac gtggaagttc
     1681 ttggaatact ttccgatgat gtagagactg ataccgtagc cccaggtgaa aacctcaaaa
     1741 tcagactgaa aggaattgaa gaagaggaga ttcttccagg gtttatactt tgtgatccta
     1801 ataatctttg tcattctgga cgcacatttg atgcccagat agtgattata gagcacaaat
     1861 ccatcatctg cccaggctat aatgcggtgc tgcatattca tacctgtatt gaggaggtgg
     1921 aaataacagc cttaatctgc ttggtagaca aaaaatcagg agaaaaaagt aagacccgac
     1981 cccgttttgt caaacaagat caagtatgca ttgctcgctt aaggacagca ggaaccatct
     2041 gccttgagac ctttaaagac ttccctcaga tgggtcgttt caccttaaga gatgagggta
     2101 agaccattgc aattggaaaa gttctgaaac tggttccaga gaaagactaa gcattttctt
     2161 gatgaccctg cacaatactg tgaggaaaat tgactgcaga agcctacttc acaccgcctt
     2221 ctcttatttt ctgcccattg ataaacctct ccccatattt tgcaaagagg aaattcacag
     2281 caaaagtcca cattatgtca gctttctcat attgagagct ctgctatgcc actgttgaat
     2341 ttttcccaag attcctgtcc ctagccctca cttcaaactc tgcttccttg gacagatttg
     2401 gcaatagctt tgtaagtgat gtggacataa ttgcctacaa taatgaaaac ctacaggaat
     2461 ttttttattt ttcattttcc ccttaggcat atttagtatt tttcccccag gcagatcatt
     2521 ctgagtgtgc gagtgtgtgt gcacatgtta caaaggcaac taccatgtta ataaaatatt
     2581 caatttgaaa tccttttcgg tatttgaatt gcttttgaat aatgtttttt atctggatgt
     2641 aacattgttg cattagcttt ttaactttcc caagtaattg aatacatttt attacttgga
     2701 cttttataaa ctctttccct acccactata aatgagacat tcacagcgtt caagtttgta
     2761 ttaaaggaaa ggattagttt gaccccttct tttgatggtt aatgcataca tgcagttaaa
     2821 tccctttatg caaatgtgac actgctttac taggtctttt agttatttat ttattttttt
     2881 tttttttgcc aatttatatt gtaacatgat ttcttgaaca cagtaagttg ttttaaaata
     2941 aaagaacagc atactgtagt ttttataagt aaagcgtaat gaaattgtac ccctagagaa
     3001 aattccatta acttgttaaa ttagtggaat taacaacaaa taaagcatgt ttgagacctg
     3061 gcaaaaattc ctctggtagt atttataaat agagctgcat gcctctagta tgaaaaccgt
     3121 atcagttgca agtgccactt ctacaagtta ctcagtttac tctttgtatc agtaacttta
     3181 aaggttggat gatccttgct ggttaaagct aaatctcaac ctagcaacta aatgaaaata
     3241 tttagaatca tcagaatctg aacagactaa aattatcagc gataagcaga atcaagcagg
     3301 gtataagttt tatctcaatt atttgaaatt gactgagttt tcttaagtgt aaagctgaaa
     3361 tttgctaacc atgtttttga tgaaccacag tgcagcattg tgtgggtttt agattgaatg
     3421 actctctgct ataattatca tgactttgaa atttttcatg agaataaatg aggaatggag
     3481 gcaatttgtg ggtttatcca aagccccctt gatgttgagt gttgccattg atcaaagata
     3541 gcctctttta gattgtgatt tctctctaaa taagatactt tgtatattta atgacaagtc
     3601 tcttccctat aattttgctc ctcagaaaag aaaagcctag acagactgta tttccagtca
     3661 cagaacataa aacttaatct aatgggtgtt ttgttttgtt ttgagacaga ggctcactct
     3721 gtcacctagg ctggagtgca gtggctcgat ctcagctcac tgcagcctcc acctcccgga
     3781 ttcaagcaat ttcctgcctc agcctcttga gtagctggga ttacaggagc cctccacgag
     3841 gcccagttaa tttttgtatc tttagtggag acgaggtttc cccctattgg tcaggctggt
     3901 ctcaaacttc tgacctcaag tgatccactc gcctcagcct ctcaaaagtg ctgggattac
     3961 aggcatgagg cgccgcactt ggcctagtgt tttcttaact gtgaaattcc cattcatttc
     4021 ttgaatgagg ctacatctta tggacagagc aaagttattg tcctacagat tcttaaaact
     4081 ataattatgg ctattgcatg aaatttaaat agattttatt atgtctgcaa atctctgggc
     4141 ttttattttt ctggaaaata taggagcttt aatcaaaaca taatagttct ttttgtaatt
     4201 ccatgttaat aaaaacaaat actagcaatt gcttgaattt taatgaatat ttaaaagttc
     4261 aagagccacg gaaatcactt ccagagataa gagttccctt tctaaataga acacattttt
     4321 aaaaaataag ttatgtttgc tactaaaaca tttacactgt tagactatta tgtgcatgtt
     4381 gccaagactc ttaagtaact tggatatcaa ctgtgaaggg cctacctcta aaaagtaaca
     4441 ggtcatacaa atacaaatgt aactgtaaaa attccactgg attcttgcat atttgcaaga
     4501 ttagattatt caaaagaaat ttcagtgcta aaattaacca gcaacataag ttctatgggc
     4561 tttgaaaatt gttctcatct ttttaaagtt gatgcatttt caatcctgct tacacaggct
     4621 gttcatttgg ataagtaaat aaaatgtcta aggtgaactt ggcattatgt ggagatgttg
     4681 gaccgttata gagcaataca aattcctatg ctgtcattct gttttctgca aatgcaaacg
     4741 tgcttatatg gtcaacagtg caaaaatagg gtagttggct gcatatttag ggtattacct
     4801 aagcatttgt tctctaacgt tgctctacta gaatgatttt tttcttgcat cttttcacat
     4861 taatgatgtt ctttatataa ctttcatgcg attatttagt tttttaaatt aataaagtga
     4921 atttaagaaa tattgaaata aacatctaag taattgccat tttaaaccct tgtttcttac
     4981 tgtgggagag ggggaaatac agcactcatt tcttgttttt aatttgcaga agtaagtgaa
     5041 aatctatgta aaatcaaacc aaaagagttg gactgagtgt gtattgtctt gagattaagt
     5101 gacaaatagt aaagtgttac tgagtaatta agcccatgta tttttttttt gtgagttgaa
     5161 aatctttgaa atatgtgata accgaatgtc aaaagttcct aaactctaac agtgcaggtt
     5221 gttcactgta acgaggtaac tcatatttgc tggttacata aactacaagt actgctctca
     5281 caatatggga ctttgaactg tgatgtagtt caacagttgc cggcatcctc tcagctgata
     5341 cgctgcgaat attttgggtt agacttgcag ccagatgcag ttttgcaacc caagaaaaaa
     5401 gttgaaccta tgatcaaaaa ctgctcccaa gatgaacctg gaaaaaaatc agctaagctc
     5461 ccttggcgat ctgcaggaac actagtaatg actggaatta ctccgtgatc tttgatgact
     5521 attacacata acagcactct agcacctttt cttactggca tggacttcct catggactgc
     5581 tacttcatgg atgatagctt cattgctttg ggtagggatt taaggtagtc aaggggaaaa
     5641 tacgcagttt tattacaggt cttaacatca ggcaactttc aactttaaaa ccctttgtga
     5701 aaaatgtggt tatagcacta tagctctgat tttaggatgg ttaaatgtta tattcattgt
     5761 tggcttacct tatcaaactg tgccattaat cctttcacag acataggtaa ggaagagaac
     5821 aaccagtgga ttcaggggac aattatctat ctccaaataa taggctttta tttcttgcag
     5881 ctaacttttt cagtgattct agcagatgcc atctagtaca tccttgatct tgtttctttc
     5941 gtgagagatc tcgccatggc agcatcttgt taagtaagtg taattgcaca tgcacaaaag
     6001 acttaactag ctttacattt agcagtcagt tggttagatt aggtttcata gtaaatgaat
     6061 aggaatagaa agaataggaa gtgtttttat tttccagtag taattccgtg gattccattt
     6121 gacccagttt actatcagtt cagttcaggt agatttggtt caacttttgg tggtttttgg
     6181 ctctaggata ttcttgactt taatatccta gaacttactg agtcttccct tcaataaata
     6241 cacttctcac atacctctaa tcctatgctt ccttgaaaca ataatgctag ctgagttgtt
     6301 tactaaggat tattataagg gcctgaaggt gtgggagtgg agattaatta aaacctttat
     6361 gttctccaat ataagggaaa agcaggttgg tactacttct gattaggcag aaaacaccag
     6421 gattccttaa gtgatccttg aaatggttat tgttttctgc cttgtcacat ttgccactgt
     6481 gccctttaaa acgatgtgga aacctcaggt ttgtggacag cacaggtgga atgacatctt
     6541 gtgcttcctg aggctcccct ctaccaggca cattagctta gtgcttcaga tgtcagccca
     6601 agtccttgtt acctcctttt cctgctgccc agggaagagt gtgtgtgctg gagctggagc
     6661 gcttgcactc ttcaggtgac tattctcacc tccatttcct ccacatgcat taggtgaaac
     6721 tgaggtctaa gcctcctgca aggtctacat tttaaggact cacacatcag gctctcagaa
     6781 atgtacacag gtattagttc tgtttgttct aaaggaaatg tgggtatctc tcaggccagg
     6841 acttagtgac tagttttcgc tagacagcag gttaatacct agatctcatt taaaaaaaaa
     6901 aaaaaaaaac aggattaaag ggaactgatc aggtttgttg agttttttag cctaattcca
     6961 aagcatggaa gagtgctcta ggtaggaaag aaagcttttt cttacgattt gtagctacct
     7021 actgtgcctg acttggtgcc tgtgtgagga ttaagccctt agtctgctct tgcaattatt
     7081 caaatgacaa attaaatttg cttttgtaat aacaataaaa gttgtcatct tcccttttga
     7141 a
//
