LOCUS       NM_001012513             839 bp    mRNA    linear   PRI 06-APR-2024
DEFINITION  Homo sapiens gastrin releasing peptide (GRP), transcript variant 3,
            mRNA.
ACCESSION   NM_001012513
VERSION     NM_001012513.3
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Li,H., Chen,X., Xu,J., Zhu,L., Li,C., Sun,X., Li,X., Guo,J., Li,J.,
            Wang,S., He,Y., Wang,H., Huang,C., Meng,X.M. and Li,J.
  TITLE     GRP/GRPR enhances alcohol-associated liver injury through the
            IRF1-mediated Caspase-1 inflammasome and NOX2-dependent ROS pathway
  JOURNAL   Hepatology 79 (2), 392-408 (2024)
   PUBMED   37409771
  REMARK    GeneRIF: GRP/GRPR enhances alcohol-associated liver injury through
            the IRF1-mediated Caspase-1 inflammasome and NOX2-dependent ROS
            pathway.
REFERENCE   2  (bases 1 to 839)
  AUTHORS   Li,L., Zhang,Q., Wang,Y. and Xu,C.
  TITLE     Evaluating the diagnostic and prognostic value of serum TuM2-PK,
            NSE, and ProGRP in small cell lung cancer
  JOURNAL   J Clin Lab Anal 37 (7), e24865 (2023)
   PUBMED   37088873
  REMARK    GeneRIF: Evaluating the diagnostic and prognostic value of serum
            TuM2-PK, NSE, and ProGRP in small cell lung cancer.
REFERENCE   3  (bases 1 to 839)
  AUTHORS   Giovanella,L., Fontana,M., Keller,F., Campenni',A., Ceriani,L. and
            Paone,G.
  TITLE     Circulating pro-gastrin releasing peptide (ProGRP) in patients with
            medullary thyroid carcinoma
  JOURNAL   Clin Chem Lab Med 59 (9), 1569-1573 (2021)
   PUBMED   33860650
  REMARK    GeneRIF: Circulating pro-gastrin releasing peptide (ProGRP) in
            patients with medullary thyroid carcinoma.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 839)
  AUTHORS   Yu,M., Yang,C., Wang,S., Zeng,Y., Chen,Z., Feng,N., Ning,C.,
            Wang,L., Xue,L. and Zhang,Z.
  TITLE     Serum ProGRP as a novel biomarker of bone metastasis in prostate
            cancer
  JOURNAL   Clin Chim Acta 510, 437-441 (2020)
   PUBMED   32791134
  REMARK    GeneRIF: Serum ProGRP as a novel biomarker of bone metastasis in
            prostate cancer.
REFERENCE   5  (bases 1 to 839)
  AUTHORS   Kayalar,O., Oztay,F. and Ongen,H.G.
  TITLE     Gastrin-releasing peptide induces fibrotic response in MRC5s and
            proliferation in A549s
  JOURNAL   Cell Commun Signal 18 (1), 96 (2020)
   PUBMED   32552754
  REMARK    GeneRIF: Gastrin-releasing peptide induces fibrotic response in
            MRC5s and proliferation in A549s.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 839)
  AUTHORS   Baraniuk,J.N., Lundgren,J.D., Shelhamer,J.H. and Kaliner,M.A.
  TITLE     Gastrin releasing peptide (GRP) binding sites in human bronchi
  JOURNAL   Neuropeptides 21 (2), 81-84 (1992)
   PUBMED   1557184
REFERENCE   7  (bases 1 to 839)
  AUTHORS   Lebacq-Verheyden,A.M., Bertness,V., Kirsch,I., Hollis,G.F.,
            McBride,O.W. and Battey,J.
  TITLE     Human gastrin-releasing peptide gene maps to chromosome band 18q21
  JOURNAL   Somat Cell Mol Genet 13 (1), 81-86 (1987)
   PUBMED   3027901
REFERENCE   8  (bases 1 to 839)
  AUTHORS   Sausville,E.A., Lebacq-Verheyden,A.M., Spindel,E.R., Cuttitta,F.,
            Gazdar,A.F. and Battey,J.F.
  TITLE     Expression of the gastrin-releasing peptide gene in human small
            cell lung cancer. Evidence for alternative processing resulting in
            three distinct mRNAs
  JOURNAL   J Biol Chem 261 (5), 2451-2457 (1986)
   PUBMED   3003116
REFERENCE   9  (bases 1 to 839)
  AUTHORS   Spindel,E.R., Zilberberg,M.D., Habener,J.F. and Chin,W.W.
  TITLE     Two prohormones for gastrin-releasing peptide are encoded by two
            mRNAs differing by 19 nucleotides
  JOURNAL   Proc Natl Acad Sci U S A 83 (1), 19-23 (1986)
   PUBMED   3001723
REFERENCE   10 (bases 1 to 839)
  AUTHORS   Spindel,E.R., Chin,W.W., Price,J., Rees,L.H., Besser,G.M. and
            Habener,J.F.
  TITLE     Cloning and characterization of cDNAs encoding human
            gastrin-releasing peptide
  JOURNAL   Proc Natl Acad Sci U S A 81 (18), 5699-5703 (1984)
   PUBMED   6207529
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC067859.7, BC004488.2 and
            CK903483.1.
            
            On May 31, 2019 this sequence version replaced NM_001012513.2.
            
            Summary: This gene encodes a member of the bombesin-like family of
            gastrin-releasing peptides. The encoded preproprotein is
            proteolytically processed to generate two peptides,
            gastrin-releasing peptide and neuromedin-C. These peptides regulate
            numerous functions of the gastrointestinal and central nervous
            systems, including release of gastrointestinal hormones, smooth
            muscle cell contraction, and epithelial cell proliferation. These
            peptides are also likely to play a role in human cancers of the
            lung, colon, stomach, pancreas, breast, and prostate. Alternative
            splicing results in multiple transcript variants, at least one of
            which encodes a preproprotein that is proteolytically processed.
            [provided by RefSeq, Jan 2016].
            
            Transcript Variant: This variant (3) uses an alternate splice site
            in the coding region, which results in a frameshift and an early
            stop codon, compared to variant 1. It encodes isoform 3 which has a
            shorter and distinct C-terminus, compared to isoform 1. This
            variant has also been identified as 'splice isoform 2',
            'pro-gastrin releasing peptide type 3', and 'gastrin-releasing
            peptide nirs variant 1'.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: DRR138508.36580.1,
                                           SRR7410570.642288.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2148874, SAMEA2151119
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-10                AC067859.7         85955-85964
            11-247              BC004488.2         1-237
            248-471             CK903483.1         234-457
            472-839             BC004488.2         481-848
FEATURES             Location/Qualifiers
     source          1..839
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="18"
                     /map="18q21.32"
     gene            1..839
                     /gene="GRP"
                     /gene_synonym="BN; GRP-10; preproGRP; proGRP"
                     /note="gastrin releasing peptide"
                     /db_xref="GeneID:2922"
                     /db_xref="HGNC:HGNC:4605"
                     /db_xref="MIM:137260"
     exon            1..247
                     /gene="GRP"
                     /gene_synonym="BN; GRP-10; preproGRP; proGRP"
                     /inference="alignment:Splign:2.1.0"
     CDS             109..525
                     /gene="GRP"
                     /gene_synonym="BN; GRP-10; preproGRP; proGRP"
                     /note="isoform 3 preproprotein is encoded by transcript
                     variant 3; bombesin; neuromedin C; pre-progastrin
                     releasing peptide; prepro-GRP; testicular tissue protein
                     Li 103"
                     /codon_start=1
                     /product="gastrin-releasing peptide isoform 3
                     preproprotein"
                     /protein_id="NP_001012531.1"
                     /db_xref="CCDS:CCDS45878.1"
                     /db_xref="GeneID:2922"
                     /db_xref="HGNC:HGNC:4605"
                     /db_xref="MIM:137260"
                     /translation="MRGRELPLVLLALVLCLAPRGRAVPLPAGGGTVLTKMYPRGNHW
                     AVGHLMGKKSTGESSSVSERGSLKQQLREYIRWEEAARNLLGLIEAKENRNHQPPQPK
                     ALGNQQPSWDSEDSSNFKDLVDSLLQVLNVKEGTPS"
     exon            248..471
                     /gene="GRP"
                     /gene_synonym="BN; GRP-10; preproGRP; proGRP"
                     /inference="alignment:Splign:2.1.0"
     exon            472..839
                     /gene="GRP"
                     /gene_synonym="BN; GRP-10; preproGRP; proGRP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      668..673
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GRP"
                     /gene_synonym="BN; GRP-10; preproGRP; proGRP"
                     /note="hexamer: ACTAAA"
     polyA_site      693
                     /gene="GRP"
                     /gene_synonym="BN; GRP-10; preproGRP; proGRP"
     regulatory      813..818
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GRP"
                     /gene_synonym="BN; GRP-10; preproGRP; proGRP"
                     /note="hexamer: AATAAA"
     polyA_site      839
                     /gene="GRP"
                     /gene_synonym="BN; GRP-10; preproGRP; proGRP"
                     /note="major polyA site"
ORIGIN      
        1 agcagtagca ccagcggctg cggcggcgga gctcctccga ggtccgggtc accagtctct
       61 gctcttccca gcctctccgg cgcgctccaa gggcttcccg tcgggaccat gcgcggccgt
      121 gagctcccgc tggtcctgct ggcgctggtc ctctgcctgg cgccccgggg gcgagcggtc
      181 ccgctgcctg cgggcggagg gaccgtgctg accaagatgt acccgcgcgg caaccactgg
      241 gcggtggggc acttaatggg gaaaaagagc acaggggagt cttcttctgt ttctgagaga
      301 gggagcctga agcagcagct gagagagtac atcaggtggg aagaagctgc aaggaatttg
      361 ctgggtctca tagaagcaaa ggagaacaga aaccaccagc cacctcaacc caaggccctg
      421 ggcaatcagc agccttcgtg ggattcagag gatagcagca acttcaaaga tttggtagac
      481 tctctgctcc aggttctcaa cgtgaaggaa ggaaccccca gctgaaccag caatgataat
      541 gatggcctct ctcaaaagag aaaaacaaaa cccctaagag actgcgttct gcaagcatca
      601 gttctacgga tcatcaacaa gatttccttg tgcaaaatat ttgactattc tgtatctttc
      661 atccttgact aaattcgtga ttttcaagca gcatcttctg gtttaaactt gtttgctgtg
      721 aacaattgtc gaaaagagtc ttccaattaa tgctttttta tatctaggct acctgttggt
      781 tagattcaag gccccgagct gttaccattc acaataaaag cttaaacaca ttgtccaaa
//
