LOCUS       NM_001278064            6846 bp    mRNA    linear   PRI 11-FEB-2025
DEFINITION  Homo sapiens glutamate metabotropic receptor 1 (GRM1), transcript
            variant 3, mRNA.
ACCESSION   NM_001278064
VERSION     NM_001278064.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6846)
  AUTHORS   Protasova,M.S., Andreeva,T.V., Klyushnikov,S.A., Illarioshkin,S.N.
            and Rogaev,E.I.
  TITLE     Genetic Variant in GRM1 Underlies Congenital Cerebellar Ataxia with
            No Obvious Intellectual Disability
  JOURNAL   Int J Mol Sci 24 (2), 1551 (2023)
   PUBMED   36675067
  REMARK    GeneRIF: Genetic Variant in GRM1 Underlies Congenital Cerebellar
            Ataxia with No Obvious Intellectual Disability.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6846)
  AUTHORS   Yousaf,H., Fatima,A., Ali,Z., Baig,S.M., Toft,M. and Iqbal,Z.
  TITLE     A Novel Nonsense Variant in GRM1 Causes Autosomal Recessive
            Spinocerebellar Ataxia 13 in a Consanguineous Pakistani Family
  JOURNAL   Genes (Basel) 13 (9), 1667 (2022)
   PUBMED   36140834
  REMARK    GeneRIF: A Novel Nonsense Variant in GRM1 Causes Autosomal
            Recessive Spinocerebellar Ataxia 13 in a Consanguineous Pakistani
            Family.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 6846)
  AUTHORS   Wu,Q.W. and Kapfhammer,J.P.
  TITLE     The Emerging Key Role of the mGluR1-PKCgamma Signaling Pathway in
            the Pathogenesis of Spinocerebellar Ataxias: A Neurodevelopmental
            Viewpoint
  JOURNAL   Int J Mol Sci 23 (16), 9169 (2022)
   PUBMED   36012439
  REMARK    GeneRIF: The Emerging Key Role of the mGluR1-PKCgamma Signaling
            Pathway in the Pathogenesis of Spinocerebellar Ataxias: A
            Neurodevelopmental Viewpoint.
            Review article
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 6846)
  AUTHORS   Ishibashi,K., Miura,Y., Wagatsuma,K., Kameyama,M. and Ishii,K.
  TITLE     Brain 11 C-ITMM PET to longitudinally assess type 1 metabotropic
            glutamate receptor availability in Alzheimer's disease
  JOURNAL   J Neuroimaging 31 (5), 864-868 (2021)
   PUBMED   34143915
  REMARK    GeneRIF: Brain (11) C-ITMM PET to longitudinally assess type 1
            metabotropic glutamate receptor availability in Alzheimer's
            disease.
REFERENCE   5  (bases 1 to 6846)
  AUTHORS   Hermans,E. and Challiss,R.A.
  TITLE     Structural, signalling and regulatory properties of the group I
            metabotropic glutamate receptors: prototypic family C
            G-protein-coupled receptors
  JOURNAL   Biochem J 359 (Pt 3), 465-484 (2001)
   PUBMED   11672421
  REMARK    Review article
REFERENCE   6  (bases 1 to 6846)
  AUTHORS   Brakeman,P.R., Lanahan,A.A., O'Brien,R., Roche,K., Barnes,C.A.,
            Huganir,R.L. and Worley,P.F.
  TITLE     Homer: a protein that selectively binds metabotropic glutamate
            receptors
  JOURNAL   Nature 386 (6622), 284-288 (1997)
   PUBMED   9069287
REFERENCE   7  (bases 1 to 6846)
  AUTHORS   Laurie,D.J., Boddeke,H.W., Hiltscher,R. and Sommer,B.
  TITLE     HmGlu1d, a novel splice variant of the human type I metabotropic
            glutamate receptor
  JOURNAL   Eur J Pharmacol 296 (2), R1-R3 (1996)
   PUBMED   8838462
  REMARK    Erratum:[Eur J Pharmacol. 1996 Apr 29;302(1-3):229. doi:
            10.1016/0014-2999(96)00144-6.. PMID: 8791012]
REFERENCE   8  (bases 1 to 6846)
  AUTHORS   Stephan,D., Bon,C., Holzwarth,J.A., Galvan,M. and Pruss,R.M.
  TITLE     Human metabotropic glutamate receptor 1: mRNA distribution,
            chromosome localization and functional expression of two splice
            variants
  JOURNAL   Neuropharmacology 35 (12), 1649-1660 (1996)
   PUBMED   9076744
REFERENCE   9  (bases 1 to 6846)
  AUTHORS   Desai,M.A., Burnett,J.P., Mayne,N.G. and Schoepp,D.D.
  TITLE     Cloning and expression of a human metabotropic glutamate receptor 1
            alpha: enhanced coupling on co-transfection with a glutamate
            transporter
  JOURNAL   Mol Pharmacol 48 (4), 648-657 (1995)
   PUBMED   7476890
REFERENCE   10 (bases 1 to 6846)
  AUTHORS   Perlman,S.
  TITLE     Hereditary Ataxia Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301317
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL096867.15, L76631.1,
            BC136280.1, AB208837.1, L76627.1 and AL035698.12.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_001278064.1.
            
            Summary: This gene encodes a metabotropic glutamate receptor that
            functions by activating phospholipase C. L-glutamate is the major
            excitatory neurotransmitter in the central nervous system and
            activates both ionotropic and metabotropic glutamate receptors.
            Glutamatergic neurotransmission is involved in most aspects of
            normal brain function and can be perturbed in many neuropathologic
            conditions. The canonical alpha isoform of the encoded protein is a
            disulfide-linked homodimer whose activity is mediated by a
            G-protein-coupled phosphatidylinositol-calcium second messenger
            system. This gene may be associated with many disease states,
            including schizophrenia, bipolar disorder, depression, and breast
            cancer. Alternative splicing results in multiple transcript
            variants encoding different isoforms. [provided by RefSeq, May
            2013].
            
            Transcript Variant: This variant (3) encodes the longest isoform
            (alpha).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660805.199463.1,
                                           SRR1660803.20848.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2145743, SAMEA2154665
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000282753.6/ ENSP00000282753.1
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-136               AL096867.15        96715-96850         c
            137-424             L76631.1           51-338
            425-4130            BC136280.1         1-3706
            4131-6840           AB208837.1         3747-6456
            6841-6843           L76627.1           6382-6384
            6844-6846           AL035698.12        135221-135223
FEATURES             Location/Qualifiers
     source          1..6846
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6q24.3"
     gene            1..6846
                     /gene="GRM1"
                     /gene_synonym="GPRC1A; MGLU1; MGLUR1; PPP1R85; SCA44;
                     SCAR13"
                     /note="glutamate metabotropic receptor 1"
                     /db_xref="GeneID:2911"
                     /db_xref="HGNC:HGNC:4593"
                     /db_xref="MIM:604473"
     exon            1..1156
                     /gene="GRM1"
                     /gene_synonym="GPRC1A; MGLU1; MGLUR1; PPP1R85; SCA44;
                     SCAR13"
                     /inference="alignment:Splign:2.1.0"
     CDS             457..4041
                     /gene="GRM1"
                     /gene_synonym="GPRC1A; MGLU1; MGLUR1; PPP1R85; SCA44;
                     SCAR13"
                     /note="isoform alpha precursor is encoded by transcript
                     variant 3; protein phosphatase 1, regulatory subunit 85;
                     glutamate receptor, metabotropic 1"
                     /codon_start=1
                     /product="metabotropic glutamate receptor 1 isoform alpha
                     precursor"
                     /protein_id="NP_001264993.1"
                     /db_xref="CCDS:CCDS5209.1"
                     /db_xref="GeneID:2911"
                     /db_xref="HGNC:HGNC:4593"
                     /db_xref="MIM:604473"
                     /translation="MVGLLLFFFPAIFLEVSLLPRSPGRKVLLAGASSQRSVARMDGD
                     VIIGALFSVHHQPPAEKVPERKCGEIREQYGIQRVEAMFHTLDKINADPVLLPNITLG
                     SEIRDSCWHSSVALEQSIEFIRDSLISIRDEKDGINRCLPDGQSLPPGRTKKPIAGVI
                     GPGSSSVAIQVQNLLQLFDIPQIAYSATSIDLSDKTLYKYFLRVVPSDTLQARAMLDI
                     VKRYNWTYVSAVHTEGNYGESGMDAFKELAAQEGLCIAHSDKIYSNAGEKSFDRLLRK
                     LRERLPKARVVVCFCEGMTVRGLLSAMRRLGVVGEFSLIGSDGWADRDEVIEGYEVEA
                     NGGITIKLQSPEVRSFDDYFLKLRLDTNTRNPWFPEFWQHRFQCRLPGHLLENPNFKR
                     ICTGNESLEENYVQDSKMGFVINAIYAMAHGLQNMHHALCPGHVGLCDAMKPIDGSKL
                     LDFLIKSSFIGVSGEEVWFDEKGDAPGRYDIMNLQYTEANRYDYVHVGTWHEGVLNID
                     DYKIQMNKSGVVRSVCSEPCLKGQIKVIRKGEVSCCWICTACKENEYVQDEFTCKACD
                     LGWWPNADLTGCEPIPVRYLEWSNIESIIAIAFSCLGILVTLFVTLIFVLYRDTPVVK
                     SSSRELCYIILAGIFLGYVCPFTLIAKPTTTSCYLQRLLVGLSSAMCYSALVTKTNRI
                     ARILAGSKKKICTRKPRFMSAWAQVIIASILISVQLTLVVTLIIMEPPMPILSYPSIK
                     EVYLICNTSNLGVVAPLGYNGLLIMSCTYYAFKTRNVPANFNEAKYIAFTMYTTCIIW
                     LAFVPIYFGSNYKIITTCFAVSLSVTVALGCMFTPKMYIIIAKPERNVRSAFTTSDVV
                     RMHVGDGKLPCRSNTFLNIFRRKKAGAGNANSNGKSVSWSEPGGGQVPKGQHMWHRLS
                     VHVKTNETACNQTAVIKPLTKSYQGSGKSLTFSDTSTKTLYNVEEEEDAQPIRFSPPG
                     SPSMVVHRRVPSAATTPPLPSHLTAEETPLFLAEPALPKGLPPPLQQQQQPPPQQKSL
                     MDQLQGVVSNFSTAIPDFHAVLAGPGGPGNGLRSLYPPPPPPQHLQMLPLQLSTFGEE
                     LVSPPADDDDDSERFKLLQEYVYEHEREGNTEEDELEEEEEDLQAASKLTPDDSPALT
                     PPSPFRDSVASGSSVPSSPVSESVLCTPPNVSYASVILRDYKQSSSTL"
     exon            1157..1406
                     /gene="GRM1"
                     /gene_synonym="GPRC1A; MGLU1; MGLUR1; PPP1R85; SCA44;
                     SCAR13"
                     /inference="alignment:Splign:2.1.0"
     exon            1407..1642
                     /gene="GRM1"
                     /gene_synonym="GPRC1A; MGLU1; MGLUR1; PPP1R85; SCA44;
                     SCAR13"
                     /inference="alignment:Splign:2.1.0"
     exon            1643..1889
                     /gene="GRM1"
                     /gene_synonym="GPRC1A; MGLU1; MGLUR1; PPP1R85; SCA44;
                     SCAR13"
                     /inference="alignment:Splign:2.1.0"
     exon            1890..2058
                     /gene="GRM1"
                     /gene_synonym="GPRC1A; MGLU1; MGLUR1; PPP1R85; SCA44;
                     SCAR13"
                     /inference="alignment:Splign:2.1.0"
     exon            2059..2185
                     /gene="GRM1"
                     /gene_synonym="GPRC1A; MGLU1; MGLUR1; PPP1R85; SCA44;
                     SCAR13"
                     /inference="alignment:Splign:2.1.0"
     exon            2186..3116
                     /gene="GRM1"
                     /gene_synonym="GPRC1A; MGLU1; MGLUR1; PPP1R85; SCA44;
                     SCAR13"
                     /inference="alignment:Splign:2.1.0"
     exon            3117..6846
                     /gene="GRM1"
                     /gene_synonym="GPRC1A; MGLU1; MGLUR1; PPP1R85; SCA44;
                     SCAR13"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6823..6828
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GRM1"
                     /gene_synonym="GPRC1A; MGLU1; MGLUR1; PPP1R85; SCA44;
                     SCAR13"
                     /note="hexamer: AATAAA"
     polyA_site      6846
                     /gene="GRM1"
                     /gene_synonym="GPRC1A; MGLU1; MGLUR1; PPP1R85; SCA44;
                     SCAR13"
                     /note="major polyA site"
ORIGIN      
        1 aagctgttcc tgcagccgat atcaggatgt gccgaaatga aacggacaag gcaactgtta
       61 acattataga ccccagagtt ttaacacagg tcctctgatg acaaggttgt gatttttctc
      121 tgtcttttgt cagcagcatc tagctcaccg ctgccaacac gacttccact gtactcttga
      181 tcaatttacc ttgatgcact accggtgaag aacggggact cgaattccct tacaaacgcc
      241 tccagcttgt agaggcggtc gtggaggacc cagaggagga gacgaagggg aaggaggcgg
      301 tggtggagga ggcaaaggcc ttggacgacc attgttggcg aggggcacca ctccgggaga
      361 ggcggcgctg ggcgtcttgg gggtgcgcgc cgggagcctg cagcgggacc agcgtgggaa
      421 cgcggctggc aggctgtgga cctcgtcctc accaccatgg tcgggctcct tttgtttttt
      481 ttcccagcga tctttttgga ggtgtccctt ctccccagaa gccccggcag gaaagtgttg
      541 ctggcaggag cgtcgtctca gcgctcggtg gccagaatgg acggagatgt catcattgga
      601 gccctcttct cagtccatca ccagcctccg gccgagaaag tgcccgagag gaagtgtggg
      661 gagatcaggg agcagtatgg catccagagg gtggaggcca tgttccacac gttggataag
      721 atcaacgcgg acccggtcct cctgcccaac atcaccctgg gcagtgagat ccgggactcc
      781 tgctggcact cttccgtggc tctggaacag agcattgagt tcattaggga ctctctgatt
      841 tccattcgag atgagaagga tgggatcaac cggtgtctgc ctgacggcca gtccctcccc
      901 ccaggcagga ctaagaagcc cattgcggga gtgatcggtc ccggctccag ctctgtagcc
      961 attcaagtgc agaacctgct ccagctcttc gacatccccc agatcgctta ttcagccaca
     1021 agcatcgacc tgagtgacaa aactttgtac aaatacttcc tgagggttgt cccttctgac
     1081 actttgcagg caagggccat gcttgacata gtcaaacgtt acaattggac ctatgtctct
     1141 gcagtccaca cggaagggaa ttatggggag agcggaatgg acgctttcaa agagctggct
     1201 gcccaggaag gcctctgtat cgcccattct gacaaaatct acagcaacgc tggggagaag
     1261 agctttgacc gactcttgcg caaactccga gagaggcttc ccaaggctag agtggtggtc
     1321 tgcttctgtg aaggcatgac agtgcgagga ctcctgagcg ccatgcggcg ccttggcgtc
     1381 gtgggcgagt tctcactcat tggaagtgat ggatgggcag acagagatga agtcattgaa
     1441 ggttatgagg tggaagccaa cgggggaatc acgataaagc tgcagtctcc agaggtcagg
     1501 tcatttgatg attatttcct gaaactgagg ctggacacta acacgaggaa tccctggttc
     1561 cctgagttct ggcaacatcg gttccagtgc cgccttccag gacaccttct ggaaaatccc
     1621 aactttaaac gaatctgcac aggcaatgaa agcttagaag aaaactatgt ccaggacagt
     1681 aagatggggt ttgtcatcaa tgccatctat gccatggcac atgggctgca gaacatgcac
     1741 catgccctct gccctggcca cgtgggcctc tgcgatgcca tgaagcccat cgacggcagc
     1801 aagctgctgg acttcctcat caagtcctca ttcattggag tatctggaga ggaggtgtgg
     1861 tttgatgaga aaggagacgc tcctggaagg tatgatatca tgaatctgca gtacactgaa
     1921 gctaatcgct atgactatgt gcacgttgga acctggcatg aaggagtgct gaacattgat
     1981 gattacaaaa tccagatgaa caagagtgga gtggtgcggt ctgtgtgcag tgagccttgc
     2041 ttaaagggcc agattaaggt tatacggaaa ggagaagtga gctgctgctg gatttgcacg
     2101 gcctgcaaag agaatgaata tgtgcaagat gagttcacct gcaaagcttg tgacttggga
     2161 tggtggccca atgcagatct aacaggctgt gagcccattc ctgtgcgcta tcttgagtgg
     2221 agcaacatcg aatccattat agccatcgcc ttttcatgcc tgggaatcct tgttaccttg
     2281 tttgtcaccc taatctttgt actgtaccgg gacacaccag tggtcaaatc ctccagtcgg
     2341 gagctctgct acatcatcct agctggcatc ttccttggtt atgtgtgccc attcactctc
     2401 attgccaaac ctactaccac ctcctgctac ctccagcgcc tcttggttgg cctctcctct
     2461 gcgatgtgct actctgcttt agtgactaaa accaatcgta ttgcacgcat cctggctggc
     2521 agcaagaaga agatctgcac ccggaagccc aggttcatga gtgcctgggc tcaggtgatc
     2581 attgcctcaa ttctgattag tgtgcaacta accctggtgg taaccctgat catcatggaa
     2641 ccccctatgc ccattctgtc ctacccaagt atcaaggaag tctaccttat ctgcaatacc
     2701 agcaacctgg gtgtggtggc ccctttgggc tacaatggac tcctcatcat gagctgtacc
     2761 tactatgcct tcaagacccg caacgtgccc gccaacttca acgaggccaa atatatcgcg
     2821 ttcaccatgt acaccacctg tatcatctgg ctagcttttg tgcccattta ctttgggagc
     2881 aactacaaga tcatcacaac ttgctttgca gtgagtctca gtgtaacagt ggctctgggg
     2941 tgcatgttca ctcccaagat gtacatcatt attgccaagc ctgagaggaa tgtccgcagt
     3001 gccttcacca cctctgatgt tgtccgcatg catgttggcg atggcaagct gccctgccgc
     3061 tccaacactt tcctcaacat cttccgaaga aagaaggcag gggcagggaa tgccaattct
     3121 aatggcaagt ctgtgtcatg gtctgaacca ggtggaggac aggtgcccaa gggacagcat
     3181 atgtggcacc gcctctctgt gcacgtgaag accaatgaga cggcctgcaa ccaaacagcc
     3241 gtcatcaagc ccctcactaa aagttaccaa ggctctggca agagcctgac cttttcagat
     3301 accagcacca agacccttta caacgtagag gaggaggagg atgcccagcc gattcgcttt
     3361 agcccgcctg gtagcccttc catggtggtg cacaggcgcg tgccaagcgc ggcgaccact
     3421 ccgcctctgc cgtcccacct gaccgcagag gagacccccc tcttcctggc cgaaccagcc
     3481 ctccccaagg gcttgccccc tcctctccag cagcagcagc aaccccctcc acagcagaaa
     3541 tcgctgatgg accagctcca gggagtggtc agcaacttca gtaccgcgat cccggatttt
     3601 cacgcggtgc tggcaggccc cggtggtccc gggaacgggc tgcggtccct gtacccgccc
     3661 ccgccacctc cgcagcacct gcagatgctg ccgctgcagc tgagcacctt tggggaggag
     3721 ctggtctccc cgcccgcgga cgacgacgac gacagcgaga ggtttaagct cctccaggag
     3781 tacgtgtatg agcacgagcg ggaagggaac acggaagaag acgaactgga agaggaggag
     3841 gaggacctgc aggcggccag caaactgacc ccggatgatt cgcctgcgct gacgcctccg
     3901 tcgcctttcc gcgactcggt ggcctcgggc agctcggtgc ccagctcccc cgtgtccgag
     3961 tcggtgctct gcacccctcc caacgtatcc tacgcctctg tcattctgcg ggactacaag
     4021 caaagctctt ccaccctgta agggggaagg gtccacatag aaaagcaaga caagccagag
     4081 atctcccaca cctccagaga tgtgcaaaca gctgggagga aaagcctggg agtggggggc
     4141 ctcgtcggga ggacaggaga ccgctgctgc tgctgccgct actgctgctg ctgccttaag
     4201 taggaagaga gggaaggaca ccaagcaaaa aatgttccag gccaggattc ggattcttga
     4261 attactcgaa gccttctctg ggaagaaagg gaattctgac aaagcacaat tccatatggt
     4321 atgtaacttt tatcacaaat caaatagtga catcacaaac ataatgtcct cttttgcaca
     4381 attgtgcata gatatatata tgcccacaca cactgggcca tgcttgccaa ggaacagccc
     4441 acgtggacat gccagtcgga tcatgagttc acctgatggc attcggagtg agctggtgga
     4501 gccagacaga gcaggtgcgg ggaagggaag ggcccaggcc agacccatcc caaacggatg
     4561 atgggatgat gggacagcag ctccttgctc agaagccctt ctccccgctg ggctgacaga
     4621 ctcctcatct tcaggagact caggaatgga gcggcacagg ggtctctctt catccactgc
     4681 aacccatcca gtgccagctt tgagattgca cttgaagaaa ggtgcatgga ccccctgctg
     4741 ctctgcagat tccctttatt taggaaaaca ggaataagag caaaattatc accaaaaagt
     4801 gcttcatcag gcgtgctaca ggaggaagga gctagaaata gaacaatcca tcagcatgag
     4861 actttgaaaa aaaaacacat gatcagcttc tcatgttcca tattcactta ttggcgattt
     4921 ggggaaaagg ccggaacaag agattgttac gagagtggca gaaacccttt tgtagattga
     4981 cttgtgtttg tgccaagcgg gctttccatt gaccttcagt taaagaacaa accatgtgac
     5041 aaaattgtta ccttccactt actgtagcaa ataataccta caagttgaac ttctaagatg
     5101 cgtatatgta caatttggtg ccattatttc tcctacgtat tagagaaaca aatccatctt
     5161 tgaatctaat ggtgtactca tagcaactat tactggttta aatgacaaat aattctatcc
     5221 tattgtcact gaagtccttg taactagcga gtgaatgtgt tcctgtgtcc ttgtatatgt
     5281 gcgatcgtaa aatttgtgca atgtaatgtc aaattgactg gtcaatgtca acctagtagt
     5341 caatctaact gcaattagaa attgtctttt gaatatacta tatatatttt ttatgttcca
     5401 ataatgtttt gtacatcatt gtcatcaata tctacagaag ctctttgacg gtttgaatac
     5461 tatggctcaa ggttttcata tgcagctcgg atggacattt ttcttctaag atggaactta
     5521 tttttcagat attttctgat gtggagatat gttattaatg aagtggtttg aaaatttgtt
     5581 atattaaaag tgcacaaaaa ctgagagtga aaataaaagg tacattttat aagcttgcac
     5641 acattattaa cacataagat tgaacaaagc atttagatta ttccaggtta tatcattttt
     5701 ttaaagattt tccacagcta cttgagtgtc taacatacag taacatctaa ctcagctaat
     5761 aatttgtaaa atctttatca atcacatttt gccttctttt aatttttatg ttcatggact
     5821 tttattcctg tgtcttggct gtcataactt tttatttctg ctatttgctg ttgtgtaata
     5881 tccatggaca tgtaatccac ttactccatc tttacaatcc ctttttacca ccaataaaag
     5941 gatttttctt gctgttttga tttcttctat tatttgtgga atgaattata ccccccttaa
     6001 atatctttgt ttatgcctta tgttcagtca tattttaata tgcttccttc atattgaagc
     6061 tgctgatttc tcagccaaaa atcatcttag aatctttaaa tatccattgc atcatttgtt
     6121 cagaatttaa catccattcc aatgttggag gcttgtatta cttatatttc atcatattct
     6181 attgccaagt ttagtcagtt ccacaccaag aatgaactgc atttccttta aaaattattt
     6241 taaaacacct ttattgaaaa gatctcatga ctgagatgtg gactttggtt ccatgttttc
     6301 attgtaagaa agcagagagc ggaaaatcaa tggctccagt gattaataga tgggttttta
     6361 gtaattgaca aattcatgag ggaaagcata tgatctcttt attagtgaat catgcttatt
     6421 ttttactctt aatgccacta atatacatcc ctaatatcac agggcttgtg cattcagatt
     6481 tttaaaaaat taggatagat aaggaaacaa cttatattca agtgtaagat gatatcaggt
     6541 tggtctaaga cttttggtga acacgttcat tcaactgtga tcactttatt actctgaatg
     6601 cctactatta tcctgattat ggggtctcct gaataaatag agtattagtc cttatgtcat
     6661 cattgttcaa aattggagat gtacacatac ataccctata ccaagagggc cgaaactctt
     6721 caccttgatg tatgttctga tacaagttgt tcagcttctt gtaaatgtgt tttccttcgg
     6781 cttgttactg ccttttgtca aataatcttg acaatgctgt ataataaata ttttctattt
     6841 attaaa
//
