LOCUS       NM_001004106            3002 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens G protein-coupled receptor kinase 6 (GRK6), transcript
            variant 1, mRNA.
ACCESSION   NM_001004106
VERSION     NM_001004106.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3002)
  AUTHORS   Stegen,M. and Frey,U.H.
  TITLE     The Role of G Protein-Coupled Receptor Kinase 6 Regulation in
            Inflammation and Pain
  JOURNAL   Int J Mol Sci 23 (24), 15880 (2022)
   PUBMED   36555521
  REMARK    GeneRIF: The Role of G Protein-Coupled Receptor Kinase 6 Regulation
            in Inflammation and Pain.
            Review article
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3002)
  AUTHORS   Song,S., Zhao,Y., Wang,X., Tong,X., Chen,X. and Xiong,Q.
  TITLE     M2 macrophages-derived exosomal miR-3917 promotes the progression
            of lung cancer via targeting GRK6
  JOURNAL   Biol Chem 404 (1), 41-57 (2022)
   PUBMED   36261031
  REMARK    GeneRIF: M2 macrophages-derived exosomal miR-3917 promotes the
            progression of lung cancer via targeting GRK6.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3002)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   4  (bases 1 to 3002)
  AUTHORS   Stegen,M., Engler,A., Ochsenfarth,C., Manthey,I., Peters,J.,
            Siffert,W. and Frey,U.H.
  TITLE     Characterization of the G protein-coupled receptor kinase 6
            promoter reveals a functional CREB binding site
  JOURNAL   PLoS One 16 (2), e0247087 (2021)
   PUBMED   33600497
  REMARK    GeneRIF: Characterization of the G protein-coupled receptor kinase
            6 promoter reveals a functional CREB binding site.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3002)
  AUTHORS   Vistrup-Parry,M., Sneddon,W.B., Bach,S., Stromgaard,K.,
            Friedman,P.A. and Mamonova,T.
  TITLE     Multisite NHERF1 phosphorylation controls GRK6A regulation of
            hormone-sensitive phosphate transport
  JOURNAL   J Biol Chem 296, 100473 (2021)
   PUBMED   33639163
  REMARK    GeneRIF: Multisite NHERF1 phosphorylation controls GRK6A regulation
            of hormone-sensitive phosphate transport.
REFERENCE   6  (bases 1 to 3002)
  AUTHORS   Bullrich,F., Druck,T., Kunapuli,P., Gomez,J., Gripp,K.W.,
            Schlegelberger,B., Lasota,J., Aronson,M., Cannizzaro,L.A.,
            Huebner,K. et al.
  TITLE     Chromosomal mapping of the genes GPRK5 and GPRK6 encoding G
            protein-coupled receptor kinases GRK5 and GRK6
  JOURNAL   Cytogenet Cell Genet 70 (3-4), 250-254 (1995)
   PUBMED   7789183
REFERENCE   7  (bases 1 to 3002)
  AUTHORS   Stoffel,R.H., Randall,R.R., Premont,R.T., Lefkowitz,R.J. and
            Inglese,J.
  TITLE     Palmitoylation of G protein-coupled receptor kinase, GRK6. Lipid
            modification diversity in the GRK family
  JOURNAL   J Biol Chem 269 (45), 27791-27794 (1994)
   PUBMED   7961702
REFERENCE   8  (bases 1 to 3002)
  AUTHORS   Loudon,R.P. and Benovic,J.L.
  TITLE     Expression, purification, and characterization of the G
            protein-coupled receptor kinase GRK6
  JOURNAL   J Biol Chem 269 (36), 22691-22697 (1994)
   PUBMED   8077221
REFERENCE   9  (bases 1 to 3002)
  AUTHORS   Haribabu,B. and Snyderman,R.
  TITLE     Identification of additional members of human G-protein-coupled
            receptor kinase multigene family
  JOURNAL   Proc Natl Acad Sci U S A 90 (20), 9398-9402 (1993)
   PUBMED   8415712
REFERENCE   10 (bases 1 to 3002)
  AUTHORS   Benovic,J.L. and Gomez,J.
  TITLE     Molecular cloning and expression of GRK6. A new member of the G
            protein-coupled receptor kinase family
  JOURNAL   J Biol Chem 268 (26), 19521-19527 (1993)
   PUBMED   8366096
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC145098.2, BI258860.1 and
            BC009277.2.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_001004106.2.
            
            Summary: This gene encodes a member of the guanine
            nucleotide-binding protein (G protein)-coupled receptor kinase
            subfamily of the Ser/Thr protein kinase family. The protein
            phosphorylates the activated forms of G protein-coupled receptors
            thus initiating their deactivation. Several transcript variants
            encoding different isoforms have been described for this gene.
            [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) uses an alternate acceptor
            splice site in the last exon, compared to variant 2, resulting in a
            shorter isoform (A) compared to isoform B.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: L16862.1, SRR1163658.111172.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000355472.10/ ENSP00000347655.5
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-10                AC145098.2         63856-63865
            11-70               BI258860.1         1-60
            71-2733             BC009277.2         1-2663
            2734-2960           AC145098.2         79803-80029
            2961-3002           AC145098.2         80030-80071
FEATURES             Location/Qualifiers
     source          1..3002
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="5"
                     /map="5q35.3"
     gene            1..3002
                     /gene="GRK6"
                     /gene_synonym="GPRK6"
                     /note="G protein-coupled receptor kinase 6"
                     /db_xref="GeneID:2870"
                     /db_xref="HGNC:HGNC:4545"
                     /db_xref="MIM:600869"
     exon            1..222
                     /gene="GRK6"
                     /gene_synonym="GPRK6"
                     /inference="alignment:Splign:2.1.0"
     CDS             171..1901
                     /gene="GRK6"
                     /gene_synonym="GPRK6"
                     /EC_number="2.7.11.16"
                     /note="isoform A is encoded by transcript variant 1"
                     /codon_start=1
                     /product="G protein-coupled receptor kinase 6 isoform A"
                     /protein_id="NP_001004106.1"
                     /db_xref="CCDS:CCDS34303.1"
                     /db_xref="GeneID:2870"
                     /db_xref="HGNC:HGNC:4545"
                     /db_xref="MIM:600869"
                     /translation="MELENIVANTVLLKAREGGGGNRKGKSKKWRQMLQFPHISQCEE
                     LRLSLERDYHSLCERQPIGRLLFREFCATRPELSRCVAFLDGVAEYEVTPDDKRKACG
                     RQLTQNFLSHTGPDLIPEVPRQLVTNCTQRLEQGPCKDLFQELTRLTHEYLSVAPFAD
                     YLDSIYFNRFLQWKWLERQPVTKNTFRQYRVLGKGGFGEVCACQVRATGKMYACKKLE
                     KKRIKKRKGEAMALNEKQILEKVNSRFVVSLAYAYETKDALCLVLTLMNGGDLKFHIY
                     HMGQAGFPEARAVFYAAEICCGLEDLHRERIVYRDLKPENILLDDHGHIRISDLGLAV
                     HVPEGQTIKGRVGTVGYMAPEVVKNERYTFSPDWWALGCLLYEMIAGQSPFQQRKKKI
                     KREEVERLVKEVPEEYSERFSPQARSLCSQLLCKDPAERLGCRGGSAREVKEHPLFKK
                     LNFKRLGAGMLEPPFKPDPQAIYCKDVLDIEQFSTVKGVELEPTDQDFYQKFATGSVP
                     IPWQNEMVETECFQELNVFGLDGSVPPDLDWKGQPPAPPKKGLLQRLFSRQDCCGNCS
                     DSEEELPTRL"
     exon            223..318
                     /gene="GRK6"
                     /gene_synonym="GPRK6"
                     /inference="alignment:Splign:2.1.0"
     exon            319..431
                     /gene="GRK6"
                     /gene_synonym="GPRK6"
                     /inference="alignment:Splign:2.1.0"
     exon            432..509
                     /gene="GRK6"
                     /gene_synonym="GPRK6"
                     /inference="alignment:Splign:2.1.0"
     exon            510..610
                     /gene="GRK6"
                     /gene_synonym="GPRK6"
                     /inference="alignment:Splign:2.1.0"
     exon            611..703
                     /gene="GRK6"
                     /gene_synonym="GPRK6"
                     /inference="alignment:Splign:2.1.0"
     exon            704..767
                     /gene="GRK6"
                     /gene_synonym="GPRK6"
                     /inference="alignment:Splign:2.1.0"
     exon            768..908
                     /gene="GRK6"
                     /gene_synonym="GPRK6"
                     /inference="alignment:Splign:2.1.0"
     exon            909..1099
                     /gene="GRK6"
                     /gene_synonym="GPRK6"
                     /inference="alignment:Splign:2.1.0"
     exon            1100..1137
                     /gene="GRK6"
                     /gene_synonym="GPRK6"
                     /inference="alignment:Splign:2.1.0"
     exon            1138..1227
                     /gene="GRK6"
                     /gene_synonym="GPRK6"
                     /inference="alignment:Splign:2.1.0"
     exon            1228..1436
                     /gene="GRK6"
                     /gene_synonym="GPRK6"
                     /inference="alignment:Splign:2.1.0"
     exon            1437..1574
                     /gene="GRK6"
                     /gene_synonym="GPRK6"
                     /inference="alignment:Splign:2.1.0"
     exon            1575..1712
                     /gene="GRK6"
                     /gene_synonym="GPRK6"
                     /inference="alignment:Splign:2.1.0"
     exon            1713..1847
                     /gene="GRK6"
                     /gene_synonym="GPRK6"
                     /inference="alignment:Splign:2.1.0"
     exon            1848..3002
                     /gene="GRK6"
                     /gene_synonym="GPRK6"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      2168
                     /gene="GRK6"
                     /gene_synonym="GPRK6"
     regulatory      2712..2717
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GRK6"
                     /gene_synonym="GPRK6"
                     /note="hexamer: AATAGA"
     polyA_site      2733
                     /gene="GRK6"
                     /gene_synonym="GPRK6"
                     /note="major polyA site"
     regulatory      2897..2902
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GRK6"
                     /gene_synonym="GPRK6"
                     /note="hexamer: ATTACA"
     polyA_site      2941
                     /gene="GRK6"
                     /gene_synonym="GPRK6"
     polyA_site      2955
                     /gene="GRK6"
                     /gene_synonym="GPRK6"
     polyA_site      2960
                     /gene="GRK6"
                     /gene_synonym="GPRK6"
     polyA_site      3002
                     /gene="GRK6"
                     /gene_synonym="GPRK6"
ORIGIN      
        1 ggagagtgcg cggctggctg cggcggccgg ggaggccggg gaggccgcgg cgcggtcact
       61 gcgagccgag ccgagccgcg ccgagccgcg ccgatcgcca tccggcctcg gcactcgcgc
      121 gcgatcccgg ccggcggcgc ggcccggcgg gccaggcggc gccacagccc atggagctcg
      181 agaacatcgt agcgaacacg gtgctactca aggcccggga aggtggcggt ggaaatcgca
      241 aaggcaaaag caagaaatgg cggcagatgc tccagttccc tcacatcagc cagtgcgaag
      301 agctgcggct cagcctcgag cgtgactatc acagcctgtg cgagcggcag cccattgggc
      361 gcctgctgtt ccgagagttc tgtgccacga ggccggagct gagccgctgc gtcgccttcc
      421 tggatggggt ggccgagtat gaagtgaccc cggatgacaa gcggaaggca tgtgggcggc
      481 agctaacgca gaattttctg agccacacgg gtcctgacct catccctgag gtcccccggc
      541 agctggtgac gaactgcacc cagcggctgg agcagggtcc ctgcaaagac cttttccagg
      601 aactcacccg gctgacccac gagtacctga gcgtggcccc ttttgccgac tacctcgaca
      661 gcatctactt caaccgtttc ctgcagtgga agtggctgga aaggcagcca gtgaccaaaa
      721 acaccttcag gcaataccga gtcctgggca aaggtggctt tggggaggtg tgcgcctgcc
      781 aggtgcgggc cacaggtaag atgtatgcct gcaagaagct agagaaaaag cggatcaaga
      841 agcggaaagg ggaggccatg gcgctgaacg agaagcagat cctggagaaa gtgaacagta
      901 ggtttgtagt gagcttggcc tacgcctatg agaccaagga cgcgctgtgc ctggtgctga
      961 cactgatgaa cgggggcgac ctcaagttcc acatctacca catgggccag gctggcttcc
     1021 ccgaagcgcg ggccgtcttc tacgccgccg agatctgctg tggcctggag gacctgcacc
     1081 gggagcgcat cgtgtacagg gacctgaagc ccgagaacat cttgctggat gaccacggcc
     1141 acatccgcat ctctgacctg ggactagctg tgcatgtgcc cgagggccag accatcaaag
     1201 ggcgtgtggg caccgtgggt tacatggctc cggaggtggt gaagaatgaa cggtacacgt
     1261 tcagccctga ctggtgggcg ctcggctgcc tcctgtacga gatgatcgca ggccagtcgc
     1321 ccttccagca gaggaagaag aagatcaagc gggaggaggt ggagcggctg gtgaaggagg
     1381 tccccgagga gtattccgag cgcttttccc cgcaggcccg ctcactttgc tcacagctcc
     1441 tctgcaagga ccctgccgaa cgcctggggt gtcgtggggg cagtgcccgc gaggtgaagg
     1501 agcaccccct ctttaagaag ctgaacttca agcggctggg agctggcatg ctggagccgc
     1561 cgttcaagcc tgacccccag gccatttact gcaaggatgt tctggacatt gaacagttct
     1621 ctacggtcaa gggcgtggag ctggagccta ccgaccagga cttctaccag aagtttgcca
     1681 caggcagtgt gcccatcccc tggcagaacg agatggtgga gaccgagtgc ttccaagagc
     1741 tgaatgtctt tgggctggat ggctcagttc ccccagacct ggactggaag ggccagccac
     1801 ctgcacctcc taaaaaggga ctgctgcaga gactcttcag tcgccaagat tgctgtggaa
     1861 actgcagcga cagcgaggaa gagctcccca cccgcctcta gcccccagcc cgaggccccc
     1921 accagcagtt ggcggtagca gctactccga gcgccgttta cagttttgca cagtgatctt
     1981 ccccattgtc cactcaagtc gtggcctggg gaacacagac ggagctgtcc ccagtgtcct
     2041 ccgtccctca gcccctggcc tggctgagtt tggcagggcc tgggccatcc ctgggacaaa
     2101 ggtgcgtccc ttcagctctt ctccgtggag ctcggggctt tctgtattta tgtatttgta
     2161 cgaatgtata tagcgaccag agcattctta attcccgccg cagacctggc gcccccgcct
     2221 tggctcctgg gggcagccag ccctggctgg gagagcggga gctggcagag gagccactgc
     2281 caaactcaag gctcctctgg cccagcttgg atggctgagg gtggtcacac ccctgagcct
     2341 tcagcactgt gctggccacc ccggcctctg agtaagactc gtgcctcccc ctgctgccct
     2401 gggctcaggc tgctaccctc tggggcccaa agctgtccct tctcagtgct tgtcagcgct
     2461 gggtctgggg cctctgtatg ccctaggcct gtgccaaagt ggccagagat tgggctgcct
     2521 gtgataccca tcagcccact gccccggccg gcccagatag gtctgcctct gccttccagc
     2581 tcccacagcc tggtccctga tactgggctc tgtcctgcag acacctcttt cagaaacgcc
     2641 caagcccagc ccctaggagg gggtggggca tccctggtca accctcaaac attccggact
     2701 cccctcataa caatagacac atgtgcccag caataatccg ccccttcctg tgtgcgcctg
     2761 tggggtgcgt gcgcgcgcgt gtgtacctgt gtgggtgaag gggatagggc gaggctgtgc
     2821 ctgtgcccca ggtcccagcc ctggcccttc ccagactgtg atggccatcc tggtcccagt
     2881 gttagggtag catgggatta cagggccctg ttttttccat atttaaagcc aatttttatt
     2941 actcgttttg tccaacgtaa gctgccacat gtctctgtgt gaatagtccg agcacaaacc
     3001 tg
//
