LOCUS       NM_182982               2372 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens G protein-coupled receptor kinase 4 (GRK4), transcript
            variant 1, mRNA.
ACCESSION   NM_182982 XM_001128361
VERSION     NM_182982.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Yang,D., Tang,M., Zhang,M., Ren,H., Li,X., Zhang,Z., He,B.,
            Peng,S., Wang,W., Fang,D., Song,Y., Xiong,Y., Liu,Z.Z., Liang,L.,
            Shi,W., Fu,C., Hu,Y., Jose,P.A., Zhou,L., Han,Y. and Zeng,C.
  TITLE     Downregulation of G protein-coupled receptor kinase 4 protects
            against kidney ischemia-reperfusion injury
  JOURNAL   Kidney Int 103 (4), 719-734 (2023)
   PUBMED   36669643
  REMARK    GeneRIF: Downregulation of G protein-coupled receptor kinase 4
            protects against kidney ischemia-reperfusion injury.
REFERENCE   2  (bases 1 to 2372)
  AUTHORS   Yang,J., Hall,J.E., Jose,P.A., Chen,K. and Zeng,C.
  TITLE     Comprehensive insights in GRK4 and hypertension: From mechanisms to
            potential therapeutics
  JOURNAL   Pharmacol Ther 239, 108194 (2022)
   PUBMED   35487286
  REMARK    GeneRIF: Comprehensive insights in GRK4 and hypertension: From
            mechanisms to potential therapeutics.
            Review article
REFERENCE   3  (bases 1 to 2372)
  AUTHORS   Cao,N., Tang,H., Tian,M., Gong,X., Xu,Z., Zhou,B., Lan,C., Chen,C.,
            Qu,S., Zheng,S., Ren,H., Fan,C., Jose,P.A., Zeng,C. and Xia,T.
  TITLE     Genetic variants of GRK4 influence circadian rhythm of blood
            pressure and response to candesartan in hypertensive patients
  JOURNAL   Clin Exp Hypertens 43 (7), 597-603 (2021)
   PUBMED   33899625
  REMARK    GeneRIF: Genetic variants of GRK4 influence circadian rhythm of
            blood pressure and response to candesartan in hypertensive
            patients.
REFERENCE   4  (bases 1 to 2372)
  AUTHORS   Jiang,W., Wang,X., Li,R., Wang,P., Shan,G., Jia,X. and Gu,M.
  TITLE     Targeted capture sequencing identifies genetic variations of GRK4
            and RDH8 in Han Chinese with essential hypertension in Xinjiang
  JOURNAL   PLoS One 16 (7), e0255311 (2021)
   PUBMED   34297769
  REMARK    GeneRIF: Targeted capture sequencing identifies genetic variations
            of GRK4 and RDH8 in Han Chinese with essential hypertension in
            Xinjiang.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2372)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   6  (bases 1 to 2372)
  AUTHORS   Lazari,M.F., Liu,X., Nakamura,K., Benovic,J.L. and Ascoli,M.
  TITLE     Role of G protein-coupled receptor kinases on the agonist-induced
            phosphorylation and internalization of the follitropin receptor
  JOURNAL   Mol Endocrinol 13 (6), 866-878 (1999)
   PUBMED   10379886
REFERENCE   7  (bases 1 to 2372)
  AUTHORS   Sallese,M., Mariggio,S., Collodel,G., Moretti,E., Piomboni,P.,
            Baccetti,B. and De Blasi,A.
  TITLE     G protein-coupled receptor kinase GRK4. Molecular analysis of the
            four isoforms and ultrastructural localization in spermatozoa and
            germinal cells
  JOURNAL   J Biol Chem 272 (15), 10188-10195 (1997)
   PUBMED   9092566
REFERENCE   8  (bases 1 to 2372)
  AUTHORS   Premont,R.T., Macrae,A.D., Stoffel,R.H., Chung,N., Pitcher,J.A.,
            Ambrose,C., Inglese,J., MacDonald,M.E. and Lefkowitz,R.J.
  TITLE     Characterization of the G protein-coupled receptor kinase GRK4.
            Identification of four splice variants
  JOURNAL   J Biol Chem 271 (11), 6403-6410 (1996)
   PUBMED   8626439
REFERENCE   9  (bases 1 to 2372)
  AUTHORS   Sallese,M., Lombardi,M.S. and De Blasi,A.
  TITLE     Two isoforms of G protein-coupled receptor kinase 4 identified by
            molecular cloning
  JOURNAL   Biochem Biophys Res Commun 199 (2), 848-854 (1994)
   PUBMED   8135832
REFERENCE   10 (bases 1 to 2372)
  AUTHORS   Ambrose,C., James,M., Barnes,G., Lin,C., Bates,G., Altherr,M.,
            Duyao,M., Groot,N., Church,D., Wasmuth,J.J. et al.
  TITLE     A novel G protein-coupled receptor kinase gene cloned from 4p16.3
  JOURNAL   Hum Mol Genet 1 (9), 697-703 (1992)
   PUBMED   1338872
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL390065.20, BI465220.1,
            BC027597.1, BM725978.1 and U33054.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_182982.2.
            
            Summary: This gene encodes a member of the guanine
            nucleotide-binding protein (G protein)-coupled receptor kinase
            subfamily of the Ser/Thr protein kinase family. The protein
            phosphorylates the activated forms of G protein-coupled receptors
            thus initiating its deactivation. This gene has been linked to both
            genetic and acquired hypertension. Several transcript variants
            encoding different isoforms have been found for this gene.
            [provided by RefSeq, Nov 2013].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (alpha).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK292390.1, BC117320.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968968, SAMEA2148093
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000398052.9/ ENSP00000381129.4
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-45                AL390065.20        109627-109671       c
            46-51               BI465220.1         48-53
            52-1956             BC027597.1         8-1912
            1957-2045           BM725978.1         178-266
            2046-2354           BC027597.1         2006-2314
            2355-2359           U33054.1           2109-2113
            2360-2372           AL390065.20        32482-32494         c
FEATURES             Location/Qualifiers
     source          1..2372
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="4"
                     /map="4p16.3"
     gene            1..2372
                     /gene="GRK4"
                     /gene_synonym="GPRK2L; GPRK4; GRK4a; IT11"
                     /note="G protein-coupled receptor kinase 4"
                     /db_xref="GeneID:2868"
                     /db_xref="HGNC:HGNC:4543"
                     /db_xref="MIM:137026"
     exon            1..552
                     /gene="GRK4"
                     /gene_synonym="GPRK2L; GPRK4; GRK4a; IT11"
                     /inference="alignment:Splign:2.1.0"
     CDS             501..2237
                     /gene="GRK4"
                     /gene_synonym="GPRK2L; GPRK4; GRK4a; IT11"
                     /EC_number="2.7.11.16"
                     /note="isoform alpha is encoded by transcript variant 1; G
                     protein-coupled receptor kinase 2-like"
                     /codon_start=1
                     /product="G protein-coupled receptor kinase 4 isoform
                     alpha"
                     /protein_id="NP_892027.2"
                     /db_xref="CCDS:CCDS33946.1"
                     /db_xref="GeneID:2868"
                     /db_xref="HGNC:HGNC:4543"
                     /db_xref="MIM:137026"
                     /translation="MELENIVANSLLLKARQGGYGKKSGRSKKWKEILTLPPVSQCSE
                     LRHSIEKDYSSLCDKQPIGRRLFRQFCDTKPTLKRHIEFLDAVAEYEVADDEDRSDCG
                     LSILDRFFNDKLAAPLPEIPPDVVTECRLGLKEENPSKKAFEECTRVAHNYLRGEPFE
                     EYQESSYFSQFLQWKWLERQPVTKNTFRHYRVLGKGGFGEVCACQVRATGKMYACKKL
                     QKKRIKKRKGEAMALNEKRILEKVQSRFVVSLAYAYETKDALCLVLTIMNGGDLKFHI
                     YNLGNPGFDEQRAVFYAAELCCGLEDLQRERIVYRDLKPENILLDDRGHIRISDLGLA
                     TEIPEGQRVRGRVGTVGYMAPEVVNNEKYTFSPDWWGLGCLIYEMIQGHSPFKKYKEK
                     VKWEEVDQRIKNDTEEYSEKFSEDAKSICRMLLTKNPSKRLGCRGEGAAGVKQHPVFK
                     DINFRRLEANMLEPPFCPDPHAVYCKDVLDIEQFSVVKGIYLDTADEDFYARFATGCV
                     SIPWQNEMIESGCFKDINKSESEEALPLDLDKNIHTPVSRPNRGFFYRLFRRGGCLTM
                     VPSEKEVEPKQC"
     exon            553..648
                     /gene="GRK4"
                     /gene_synonym="GPRK2L; GPRK4; GRK4a; IT11"
                     /inference="alignment:Splign:2.1.0"
     exon            649..761
                     /gene="GRK4"
                     /gene_synonym="GPRK2L; GPRK4; GRK4a; IT11"
                     /inference="alignment:Splign:2.1.0"
     exon            762..839
                     /gene="GRK4"
                     /gene_synonym="GPRK2L; GPRK4; GRK4a; IT11"
                     /inference="alignment:Splign:2.1.0"
     exon            840..943
                     /gene="GRK4"
                     /gene_synonym="GPRK2L; GPRK4; GRK4a; IT11"
                     /inference="alignment:Splign:2.1.0"
     exon            944..1036
                     /gene="GRK4"
                     /gene_synonym="GPRK2L; GPRK4; GRK4a; IT11"
                     /inference="alignment:Splign:2.1.0"
     exon            1037..1100
                     /gene="GRK4"
                     /gene_synonym="GPRK2L; GPRK4; GRK4a; IT11"
                     /inference="alignment:Splign:2.1.0"
     exon            1101..1241
                     /gene="GRK4"
                     /gene_synonym="GPRK2L; GPRK4; GRK4a; IT11"
                     /inference="alignment:Splign:2.1.0"
     exon            1242..1432
                     /gene="GRK4"
                     /gene_synonym="GPRK2L; GPRK4; GRK4a; IT11"
                     /inference="alignment:Splign:2.1.0"
     exon            1433..1470
                     /gene="GRK4"
                     /gene_synonym="GPRK2L; GPRK4; GRK4a; IT11"
                     /inference="alignment:Splign:2.1.0"
     exon            1471..1560
                     /gene="GRK4"
                     /gene_synonym="GPRK2L; GPRK4; GRK4a; IT11"
                     /inference="alignment:Splign:2.1.0"
     exon            1561..1769
                     /gene="GRK4"
                     /gene_synonym="GPRK2L; GPRK4; GRK4a; IT11"
                     /inference="alignment:Splign:2.1.0"
     exon            1770..1907
                     /gene="GRK4"
                     /gene_synonym="GPRK2L; GPRK4; GRK4a; IT11"
                     /inference="alignment:Splign:2.1.0"
     exon            1908..2045
                     /gene="GRK4"
                     /gene_synonym="GPRK2L; GPRK4; GRK4a; IT11"
                     /inference="alignment:Splign:2.1.0"
     exon            2046..2183
                     /gene="GRK4"
                     /gene_synonym="GPRK2L; GPRK4; GRK4a; IT11"
                     /inference="alignment:Splign:2.1.0"
     exon            2184..2372
                     /gene="GRK4"
                     /gene_synonym="GPRK2L; GPRK4; GRK4a; IT11"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2321..2326
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GRK4"
                     /gene_synonym="GPRK2L; GPRK4; GRK4a; IT11"
                     /note="hexamer: AATACA"
     regulatory      2331..2336
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GRK4"
                     /gene_synonym="GPRK2L; GPRK4; GRK4a; IT11"
                     /note="hexamer: AATAAA"
     polyA_site      2337
                     /gene="GRK4"
                     /gene_synonym="GPRK2L; GPRK4; GRK4a; IT11"
                     /note="major polyA site"
     polyA_site      2372
                     /gene="GRK4"
                     /gene_synonym="GPRK2L; GPRK4; GRK4a; IT11"
                     /note="major polyA site"
ORIGIN      
        1 gtagtgcccc ggccgcggcg gcgaggggcg ctccctcttc agctaagccg ttagcgccga
       61 gcccgcccgg gagcgggtcg ccggccgcgg cgggcgcccc tgccagtgag cccctgtccg
      121 aagtgagcca cggcattgac tcggggctgc ccgggggcag ggcactgagg agggagttgc
      181 gcgcgcgagg cgagggcgat ggggccaaga agaaccgggg cgatagcgcg gcagcggtga
      241 cagctgggac ccgccgcggt cgggctgccc cctcccctcg ccccgaccgc tcccctgctg
      301 gtgagggcct gcggaggcgg cggcggcggc gcccttggtg gcagtggtgg cggcggagca
      361 gcctcccggg atcgtgtccg gagctcgagg agagggtggt gcccggcgag ctatgcacgg
      421 gggcggcggc gtctcctcct gttccgcctc ctcagtctcc tcggtctcgc agaatccgcc
      481 ggcggcggcg gcgccaggac atggagctcg agaacatcgt ggccaactcg ctgctgctga
      541 aagcgcgtca aggaggatat ggcaaaaaaa gtggtcgtag taaaaaatgg aaggagatac
      601 tgacactgcc tcctgtcagc cagtgcagtg agcttagaca ttccattgaa aaggattata
      661 gcagtctttg tgacaagcaa ccgataggaa gacgtctctt caggcagttc tgtgatacca
      721 aacccactct aaagaggcac attgaattct tggatgcagt ggcagaatat gaagttgccg
      781 atgatgagga ccgaagtgat tgtggactgt caatcttaga tagattcttc aatgataagt
      841 tggcagcccc tttaccagaa atacctccag atgttgtgac agaatgtaga ttgggactga
      901 aggaggagaa cccttccaaa aaagcctttg aggaatgtac tagagttgcc cataactacc
      961 taagagggga accatttgaa gaataccaag aaagctcata tttttctcag tttttacaat
     1021 ggaaatggct ggaaaggcaa cccgtaacaa agaacacatt tagacattac agagttctag
     1081 gaaaaggcgg atttggagag gtttgcgcct gtcaagtgcg agccacagga aaaatgtatg
     1141 cctgcaaaaa gctacaaaaa aaaagaataa agaagaggaa aggtgaagct atggctctaa
     1201 atgagaaaag aattctggag aaagtgcaaa gtagattcgt agttagttta gcctacgctt
     1261 atgaaaccaa agatgccttg tgcttggtgc tcaccattat gaatggaggg gatttgaagt
     1321 ttcacattta caacctgggc aatcccggct ttgatgagca gagagccgtt ttctatgctg
     1381 cagagctgtg ttgcggcttg gaagatttac agagggaaag aattgtatac agagacttga
     1441 agcctgagaa tattctcctt gatgatcgtg gacacatccg gatttcagac ctcggtttgg
     1501 ccacagagat cccagaagga cagagggttc gaggaagagt tggaacagtc ggctacatgg
     1561 cacctgaagt tgtcaataat gaaaagtata cgtttagtcc cgattggtgg ggacttggct
     1621 gtctgatcta tgaaatgatt cagggacatt ctccattcaa aaaatacaaa gagaaagtca
     1681 aatgggagga ggtcgatcaa agaatcaaga atgataccga ggagtattct gagaagtttt
     1741 cagaggatgc caaatctatc tgcaggatgt tactcaccaa gaatccaagc aagcggctgg
     1801 gctgcagggg cgagggagcg gctggggtga agcagcaccc cgtgttcaag gacatcaact
     1861 tcaggaggct ggaggcaaac atgctggagc cccctttctg tcctgatcct catgccgttt
     1921 actgtaagga cgtcctggat atcgagcagt tctcggtggt gaaagggatc tacctggaca
     1981 ccgcagatga agacttctat gctcggtttg ctaccgggtg tgtctccatc ccctggcaga
     2041 atgagatgat cgaatctggg tgtttcaaag acatcaacaa aagtgaaagt gaggaagctt
     2101 tgccattaga tctagacaag aacatacata ccccggtttc cagaccaaac agaggcttct
     2161 tctatagact cttcagaaga gggggctgcc tgaccatggt ccccagtgag aaggaagtgg
     2221 aacccaagca atgctgagca ccccggtgcg gaccacagag cagaccctgg cgccaggaag
     2281 gagcatgtgt tagcgtctcg tcccacctgg aattgtaata aatacatcta aataaaacat
     2341 gccttgggag tgtacagacc tttctgcact aa
//
