LOCUS       NP_892027                578 aa            linear   PRI 19-SEP-2024
DEFINITION  G protein-coupled receptor kinase 4 isoform alpha [Homo sapiens].
ACCESSION   NP_892027 XP_001128361
VERSION     NP_892027.2
DBSOURCE    REFSEQ: accession NM_182982.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (residues 1 to 578)
  AUTHORS   Yang,D., Tang,M., Zhang,M., Ren,H., Li,X., Zhang,Z., He,B.,
            Peng,S., Wang,W., Fang,D., Song,Y., Xiong,Y., Liu,Z.Z., Liang,L.,
            Shi,W., Fu,C., Hu,Y., Jose,P.A., Zhou,L., Han,Y. and Zeng,C.
  TITLE     Downregulation of G protein-coupled receptor kinase 4 protects
            against kidney ischemia-reperfusion injury
  JOURNAL   Kidney Int 103 (4), 719-734 (2023)
   PUBMED   36669643
  REMARK    GeneRIF: Downregulation of G protein-coupled receptor kinase 4
            protects against kidney ischemia-reperfusion injury.
REFERENCE   2  (residues 1 to 578)
  AUTHORS   Yang,J., Hall,J.E., Jose,P.A., Chen,K. and Zeng,C.
  TITLE     Comprehensive insights in GRK4 and hypertension: From mechanisms to
            potential therapeutics
  JOURNAL   Pharmacol Ther 239, 108194 (2022)
   PUBMED   35487286
  REMARK    GeneRIF: Comprehensive insights in GRK4 and hypertension: From
            mechanisms to potential therapeutics.
            Review article
REFERENCE   3  (residues 1 to 578)
  AUTHORS   Cao,N., Tang,H., Tian,M., Gong,X., Xu,Z., Zhou,B., Lan,C., Chen,C.,
            Qu,S., Zheng,S., Ren,H., Fan,C., Jose,P.A., Zeng,C. and Xia,T.
  TITLE     Genetic variants of GRK4 influence circadian rhythm of blood
            pressure and response to candesartan in hypertensive patients
  JOURNAL   Clin Exp Hypertens 43 (7), 597-603 (2021)
   PUBMED   33899625
  REMARK    GeneRIF: Genetic variants of GRK4 influence circadian rhythm of
            blood pressure and response to candesartan in hypertensive
            patients.
REFERENCE   4  (residues 1 to 578)
  AUTHORS   Jiang,W., Wang,X., Li,R., Wang,P., Shan,G., Jia,X. and Gu,M.
  TITLE     Targeted capture sequencing identifies genetic variations of GRK4
            and RDH8 in Han Chinese with essential hypertension in Xinjiang
  JOURNAL   PLoS One 16 (7), e0255311 (2021)
   PUBMED   34297769
  REMARK    GeneRIF: Targeted capture sequencing identifies genetic variations
            of GRK4 and RDH8 in Han Chinese with essential hypertension in
            Xinjiang.
            Publication Status: Online-Only
REFERENCE   5  (residues 1 to 578)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   6  (residues 1 to 578)
  AUTHORS   Lazari,M.F., Liu,X., Nakamura,K., Benovic,J.L. and Ascoli,M.
  TITLE     Role of G protein-coupled receptor kinases on the agonist-induced
            phosphorylation and internalization of the follitropin receptor
  JOURNAL   Mol Endocrinol 13 (6), 866-878 (1999)
   PUBMED   10379886
REFERENCE   7  (residues 1 to 578)
  AUTHORS   Sallese,M., Mariggio,S., Collodel,G., Moretti,E., Piomboni,P.,
            Baccetti,B. and De Blasi,A.
  TITLE     G protein-coupled receptor kinase GRK4. Molecular analysis of the
            four isoforms and ultrastructural localization in spermatozoa and
            germinal cells
  JOURNAL   J Biol Chem 272 (15), 10188-10195 (1997)
   PUBMED   9092566
REFERENCE   8  (residues 1 to 578)
  AUTHORS   Premont,R.T., Macrae,A.D., Stoffel,R.H., Chung,N., Pitcher,J.A.,
            Ambrose,C., Inglese,J., MacDonald,M.E. and Lefkowitz,R.J.
  TITLE     Characterization of the G protein-coupled receptor kinase GRK4.
            Identification of four splice variants
  JOURNAL   J Biol Chem 271 (11), 6403-6410 (1996)
   PUBMED   8626439
REFERENCE   9  (residues 1 to 578)
  AUTHORS   Sallese,M., Lombardi,M.S. and De Blasi,A.
  TITLE     Two isoforms of G protein-coupled receptor kinase 4 identified by
            molecular cloning
  JOURNAL   Biochem Biophys Res Commun 199 (2), 848-854 (1994)
   PUBMED   8135832
REFERENCE   10 (residues 1 to 578)
  AUTHORS   Ambrose,C., James,M., Barnes,G., Lin,C., Bates,G., Altherr,M.,
            Duyao,M., Groot,N., Church,D., Wasmuth,J.J. et al.
  TITLE     A novel G protein-coupled receptor kinase gene cloned from 4p16.3
  JOURNAL   Hum Mol Genet 1 (9), 697-703 (1992)
   PUBMED   1338872
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL390065.20, BI465220.1,
            BC027597.1, BM725978.1 and U33054.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On or before Sep 20, 2006 this sequence version replaced
            XP_001128361.1, NP_892027.1.
            
            Summary: This gene encodes a member of the guanine
            nucleotide-binding protein (G protein)-coupled receptor kinase
            subfamily of the Ser/Thr protein kinase family. The protein
            phosphorylates the activated forms of G protein-coupled receptors
            thus initiating its deactivation. This gene has been linked to both
            genetic and acquired hypertension. Several transcript variants
            encoding different isoforms have been found for this gene.
            [provided by RefSeq, Nov 2013].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (alpha).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK292390.1, BC117320.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968968, SAMEA2148093
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000398052.9/ ENSP00000381129.4
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Homo sapiens"
                     /db_xref="taxon:9606"
                     /chromosome="4"
                     /map="4p16.3"
     Protein         1..578
                     /product="G protein-coupled receptor kinase 4 isoform
                     alpha"
                     /EC_number="2.7.11.16"
                     /note="G protein-coupled receptor kinase 2-like"
                     /calculated_mol_wt=66452
     Region          52..184
                     /region_name="RGS"
                     /note="Regulator of G protein signaling (RGS) domain
                     superfamily; cl02565"
                     /db_xref="CDD:470619"
     Region          186..470
                     /region_name="STKc_GRK4"
                     /note="Catalytic domain of the Serine/Threonine Kinase, G
                     protein-coupled Receptor Kinase 4; cd05631"
                     /db_xref="CDD:173720"
     Site            order(193..197,201,214,216,248,264..265,267,271,273,312,
                     314,316..317,319,329..330,333,346..351,378,384,387)
                     /site_type="active"
                     /db_xref="CDD:173720"
     Site            order(193..197,201,214,216,265..267,319,330)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:173720"
     Site            order(197,271,273,312,314,316,333,346..351,378,384,387)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:173720"
     Site            329..351
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:173720"
     CDS             1..578
                     /gene="GRK4"
                     /gene_synonym="GPRK2L; GPRK4; GRK4a; IT11"
                     /coded_by="NM_182982.3:501..2237"
                     /note="isoform alpha is encoded by transcript variant 1"
                     /db_xref="CCDS:CCDS33946.1"
                     /db_xref="GeneID:2868"
                     /db_xref="HGNC:HGNC:4543"
                     /db_xref="MIM:137026"
ORIGIN      
        1 melenivans lllkarqggy gkksgrskkw keiltlppvs qcselrhsie kdysslcdkq
       61 pigrrlfrqf cdtkptlkrh iefldavaey evaddedrsd cglsildrff ndklaaplpe
      121 ippdvvtecr lglkeenpsk kafeectrva hnylrgepfe eyqessyfsq flqwkwlerq
      181 pvtkntfrhy rvlgkggfge vcacqvratg kmyackklqk krikkrkgea malnekrile
      241 kvqsrfvvsl ayayetkdal clvltimngg dlkfhiynlg npgfdeqrav fyaaelccgl
      301 edlqrerivy rdlkpenill ddrghirisd lglateipeg qrvrgrvgtv gymapevvnn
      361 ekytfspdww glgcliyemi qghspfkkyk ekvkweevdq rikndteeys ekfsedaksi
      421 crmlltknps krlgcrgega agvkqhpvfk dinfrrlean mleppfcpdp havyckdvld
      481 ieqfsvvkgi yldtadedfy arfatgcvsi pwqnemiesg cfkdinkses eealpldldk
      541 nihtpvsrpn rgffyrlfrr ggcltmvpse kevepkqc
//
