LOCUS       NM_005160               9277 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens G protein-coupled receptor kinase 3 (GRK3), transcript
            variant 1, mRNA.
ACCESSION   NM_005160
VERSION     NM_005160.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 9277)
  AUTHORS   Naderinezhad,S., Zhang,G., Wang,Z., Zheng,D., Hulsurkar,M.,
            Bakhoum,M., Su,N., Yang,H., Shen,T. and Li,W.
  TITLE     A novel GRK3-HDAC2 regulatory pathway is a key direct link between
            neuroendocrine differentiation and angiogenesis in prostate cancer
            progression
  JOURNAL   Cancer Lett 571, 216333 (2023)
   PUBMED   37543278
  REMARK    GeneRIF: A novel GRK3-HDAC2 regulatory pathway is a key direct link
            between neuroendocrine differentiation and angiogenesis in prostate
            cancer progression.
REFERENCE   2  (bases 1 to 9277)
  AUTHORS   Wang,C., Chen,S., Li,X., Fan,L., Zhou,Z., Zhang,M., Shao,Y.,
            Shang,Z. and Niu,Y.
  TITLE     TEAD3 inhibits the proliferation and metastasis of prostate cancer
            via suppressing ADRBK2
  JOURNAL   Biochem Biophys Res Commun 654, 120-127 (2023)
   PUBMED   36907139
  REMARK    GeneRIF: TEAD3 inhibits the proliferation and metastasis of
            prostate cancer via suppressing ADRBK2.
REFERENCE   3  (bases 1 to 9277)
  AUTHORS   Rabjohns,E.M., Rampersad,R.R., Ghosh,A., Hurst,K., Eudy,A.M.,
            Brozowski,J.M., Lee,H.H., Ren,Y., Mirando,A., Gladman,J.,
            Bowser,J.L., Berg,K., Wani,S., Ralston,S.H., Hilton,M.J. and
            Tarrant,T.K.
  TITLE     Aged G Protein-Coupled Receptor Kinase 3 (Grk3)-Deficient Mice
            Exhibit Enhanced Osteoclastogenesis and Develop Bone Lesions
            Analogous to Human Paget's Disease of Bone
  JOURNAL   Cells 12 (7), 981 (2023)
   PUBMED   37048054
  REMARK    GeneRIF: Aged G Protein-Coupled Receptor Kinase 3 (Grk3)-Deficient
            Mice Exhibit Enhanced Osteoclastogenesis and Develop Bone Lesions
            Analogous to Human Paget's Disease of Bone.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 9277)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   5  (bases 1 to 9277)
  AUTHORS   Moller,T.C., Pedersen,M.F., van Senten,J.R., Seiersen,S.D.,
            Mathiesen,J.M., Bouvier,M. and Brauner-Osborne,H.
  TITLE     Dissecting the roles of GRK2 and GRK3 in mu-opioid receptor
            internalization and beta-arrestin2 recruitment using
            CRISPR/Cas9-edited HEK293 cells
  JOURNAL   Sci Rep 10 (1), 17395 (2020)
   PUBMED   33060647
  REMARK    GeneRIF: Dissecting the roles of GRK2 and GRK3 in mu-opioid
            receptor internalization and beta-arrestin2 recruitment using
            CRISPR/Cas9-edited HEK293 cells.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 9277)
  AUTHORS   Premont,R.T., Claing,A., Vitale,N., Freeman,J.L., Pitcher,J.A.,
            Patton,W.A., Moss,J., Vaughan,M. and Lefkowitz,R.J.
  TITLE     beta2-Adrenergic receptor regulation by GIT1, a G protein-coupled
            receptor kinase-associated ADP ribosylation factor
            GTPase-activating protein
  JOURNAL   Proc Natl Acad Sci U S A 95 (24), 14082-14087 (1998)
   PUBMED   9826657
REFERENCE   7  (bases 1 to 9277)
  AUTHORS   Oppermann,M., Freedman,N.J., Alexander,R.W. and Lefkowitz,R.J.
  TITLE     Phosphorylation of the type 1A angiotensin II receptor by G
            protein-coupled receptor kinases and protein kinase C
  JOURNAL   J Biol Chem 271 (22), 13266-13272 (1996)
   PUBMED   8662816
REFERENCE   8  (bases 1 to 9277)
  AUTHORS   Calabrese,G., Sallese,M., Stornaiuolo,A., Stuppia,L., Palka,G. and
            De Blasi,A.
  TITLE     Chromosome mapping of the human arrestin (SAG), beta-arrestin 2
            (ARRB2), and beta-adrenergic receptor kinase 2 (ADRBK2) genes
  JOURNAL   Genomics 23 (1), 286-288 (1994)
   PUBMED   7695743
REFERENCE   9  (bases 1 to 9277)
  AUTHORS   Parruti,G., Ambrosini,G., Sallese,M. and De Blasi,A.
  TITLE     Molecular cloning, functional expression and mRNA analysis of human
            beta-adrenergic receptor kinase 2
  JOURNAL   Biochem Biophys Res Commun 190 (2), 475-481 (1993)
   PUBMED   8427589
REFERENCE   10 (bases 1 to 9277)
  AUTHORS   Benovic,J.L., Onorato,J.J., Arriza,J.L., Stone,W.C., Lohse,M.,
            Jenkins,N.A., Gilbert,D.J., Copeland,N.G., Caron,M.G. and
            Lefkowitz,R.J.
  TITLE     Cloning, expression, and chromosomal localization of
            beta-adrenergic receptor kinase 2. A new member of the receptor
            kinase family
  JOURNAL   J Biol Chem 266 (23), 14939-14946 (1991)
   PUBMED   1869533
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL022329.9, BC036797.1,
            BX387339.2 and BC063545.1.
            
            On Nov 23, 2018 this sequence version replaced NM_005160.3.
            
            Summary: The beta-adrenergic receptor kinase specifically
            phosphorylates the agonist-occupied form of the beta-adrenergic and
            related G protein-coupled receptors. Overall, the beta adrenergic
            receptor kinase 2 has 85% amino acid similarity with beta
            adrenergic receptor kinase 1, with the protein kinase catalytic
            domain having 95% similarity. These data suggest the existence of a
            family of receptor kinases which may serve broadly to regulate
            receptor function. [provided by RefSeq, Jul 2008].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data because no single transcript was available
            for the full length of the gene. The extent of this transcript is
            supported by transcript alignments and ESTs.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803615.49367.1,
                                           SRR5189667.339304.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000324198.11/ ENSP00000317578.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-219               AL022329.9         15417-15635
            220-287             BC036797.1         1-68
            288-343             BX387339.2         114-169
            344-1526            BC036797.1         69-1251
            1527-3002           BC036797.1         1402-2877
            3003-7951           AL022329.9         173762-178710
            7952-9275           BC063545.1         1-1324
            9276-9277           AL022329.9         180035-180036
FEATURES             Location/Qualifiers
     source          1..9277
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="22"
                     /map="22q12.1"
     gene            1..9277
                     /gene="GRK3"
                     /gene_synonym="ADRBK2; BARK2"
                     /note="G protein-coupled receptor kinase 3"
                     /db_xref="GeneID:157"
                     /db_xref="HGNC:HGNC:290"
                     /db_xref="MIM:109636"
     exon            1..479
                     /gene="GRK3"
                     /gene_synonym="ADRBK2; BARK2"
                     /inference="alignment:Splign:2.1.0"
     CDS             367..2433
                     /gene="GRK3"
                     /gene_synonym="ADRBK2; BARK2"
                     /EC_number="2.7.11.15"
                     /note="isoform 1 is encoded by transcript variant 1;
                     beta-adrenergic receptor kinase 2; beta-ARK-2; adrenergic,
                     beta, receptor kinase 2"
                     /codon_start=1
                     /product="G protein-coupled receptor kinase 3 isoform 1"
                     /protein_id="NP_005151.2"
                     /db_xref="CCDS:CCDS13832.1"
                     /db_xref="GeneID:157"
                     /db_xref="HGNC:HGNC:290"
                     /db_xref="MIM:109636"
                     /translation="MADLEAVLADVSYLMAMEKSKATPAARASKRIVLPEPSIRSVMQ
                     KYLAERNEITFDKIFNQKIGFLLFKDFCLNEINEAVPQVKFYEEIKEYEKLDNEEDRL
                     CRSRQIYDAYIMKELLSCSHPFSKQAVEHVQSHLSKKQVTSTLFQPYIEEICESLRGD
                     IFQKFMESDKFTRFCQWKNVELNIHLTMNEFSVHRIIGRGGFGEVYGCRKADTGKMYA
                     MKCLDKKRIKMKQGETLALNERIMLSLVSTGDCPFIVCMTYAFHTPDKLCFILDLMNG
                     GDLHYHLSQHGVFSEKEMRFYATEIILGLEHMHNRFVVYRDLKPANILLDEHGHARIS
                     DLGLACDFSKKKPHASVGTHGYMAPEVLQKGTAYDSSADWFSLGCMLFKLLRGHSPFR
                     QHKTKDKHEIDRMTLTVNVELPDTFSPELKSLLEGLLQRDVSKRLGCHGGGSQEVKEH
                     SFFKGVDWQHVYLQKYPPPLIPPRGEVNAADAFDIGSFDEEDTKGIKLLDCDQELYKN
                     FPLVISERWQQEVTETVYEAVNADTDKIEARKRAKNKQLGHEEDYALGKDCIMHGYML
                     KLGNPFLTQWQRRYFYLFPNRLEWRGEGESRQNLLTMEQILSVEETQIKDKKCILFRI
                     KGGKQFVLQCESDPEFVQWKKELNETFKEAQRLLRRAPKFLNKPRSGTVELPKPSLCH
                     RNSNGL"
     exon            480..556
                     /gene="GRK3"
                     /gene_synonym="ADRBK2; BARK2"
                     /inference="alignment:Splign:2.1.0"
     exon            557..630
                     /gene="GRK3"
                     /gene_synonym="ADRBK2; BARK2"
                     /inference="alignment:Splign:2.1.0"
     exon            631..732
                     /gene="GRK3"
                     /gene_synonym="ADRBK2; BARK2"
                     /inference="alignment:Splign:2.1.0"
     exon            733..807
                     /gene="GRK3"
                     /gene_synonym="ADRBK2; BARK2"
                     /inference="alignment:Splign:2.1.0"
     exon            808..869
                     /gene="GRK3"
                     /gene_synonym="ADRBK2; BARK2"
                     /inference="alignment:Splign:2.1.0"
     exon            870..921
                     /gene="GRK3"
                     /gene_synonym="ADRBK2; BARK2"
                     /inference="alignment:Splign:2.1.0"
     exon            922..1013
                     /gene="GRK3"
                     /gene_synonym="ADRBK2; BARK2"
                     /inference="alignment:Splign:2.1.0"
     exon            1014..1113
                     /gene="GRK3"
                     /gene_synonym="ADRBK2; BARK2"
                     /inference="alignment:Splign:2.1.0"
     exon            1114..1192
                     /gene="GRK3"
                     /gene_synonym="ADRBK2; BARK2"
                     /inference="alignment:Splign:2.1.0"
     exon            1193..1323
                     /gene="GRK3"
                     /gene_synonym="ADRBK2; BARK2"
                     /inference="alignment:Splign:2.1.0"
     exon            1324..1418
                     /gene="GRK3"
                     /gene_synonym="ADRBK2; BARK2"
                     /inference="alignment:Splign:2.1.0"
     exon            1419..1526
                     /gene="GRK3"
                     /gene_synonym="ADRBK2; BARK2"
                     /inference="alignment:Splign:2.1.0"
     exon            1527..1593
                     /gene="GRK3"
                     /gene_synonym="ADRBK2; BARK2"
                     /inference="alignment:Splign:2.1.0"
     exon            1594..1694
                     /gene="GRK3"
                     /gene_synonym="ADRBK2; BARK2"
                     /inference="alignment:Splign:2.1.0"
     exon            1695..1761
                     /gene="GRK3"
                     /gene_synonym="ADRBK2; BARK2"
                     /inference="alignment:Splign:2.1.0"
     exon            1762..1857
                     /gene="GRK3"
                     /gene_synonym="ADRBK2; BARK2"
                     /inference="alignment:Splign:2.1.0"
     exon            1858..2020
                     /gene="GRK3"
                     /gene_synonym="ADRBK2; BARK2"
                     /inference="alignment:Splign:2.1.0"
     exon            2021..2157
                     /gene="GRK3"
                     /gene_synonym="ADRBK2; BARK2"
                     /inference="alignment:Splign:2.1.0"
     exon            2158..2271
                     /gene="GRK3"
                     /gene_synonym="ADRBK2; BARK2"
                     /inference="alignment:Splign:2.1.0"
     exon            2272..9277
                     /gene="GRK3"
                     /gene_synonym="ADRBK2; BARK2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2628..2633
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GRK3"
                     /gene_synonym="ADRBK2; BARK2"
                     /note="hexamer: AATAAA"
     polyA_site      2653
                     /gene="GRK3"
                     /gene_synonym="ADRBK2; BARK2"
     regulatory      9255..9260
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GRK3"
                     /gene_synonym="ADRBK2; BARK2"
                     /note="hexamer: AATAAA"
     polyA_site      9277
                     /gene="GRK3"
                     /gene_synonym="ADRBK2; BARK2"
                     /note="major polyA site"
ORIGIN      
        1 acttggtcgg gaggcgccgg cccagcgagg ccgctgggac tgtgcactga ggggcgctga
       61 ccgttggacg cgcgctcccc gcagaccctc gctgaaggag cagggggcgg cgcgcgtgcg
      121 cggggcgccg ggcggcgcgc gagggggcgg agcggggagg cgtgctgcga ccccggccgg
      181 ctacagcctg cggcgcgcgc agagcgctag tggggcgcgc ggcgcgcgcg cggggcgggg
      241 gcgcgcggag gggggggctg ccccggggcg gcccccccag gtcggggcgc ggcgggcggc
      301 ggcggcgggc gcgcgtcccg tccaggtccg gagtaaccgc cgccgccgcc gccaaagctc
      361 gccaacatgg cggacctgga ggctgtgctg gccgatgtca gttacctgat ggccatggag
      421 aagagcaagg cgaccccggc cgcccgcgcc agcaagagga tcgtcctgcc ggagcccagt
      481 atccggagtg tgatgcagaa gtaccttgca gagagaaatg aaataacctt tgacaagatt
      541 ttcaatcaga aaattggttt cttgctattt aaagattttt gtttgaatga aattaatgaa
      601 gctgtacctc aggtgaagtt ttatgaagag ataaaggaat atgaaaaact tgataatgag
      661 gaagaccgcc tttgcagaag tcgacaaatt tatgatgcct acatcatgaa ggaacttctt
      721 tcctgttcac atcctttctc aaagcaagct gtagaacacg tacaaagtca tttatccaag
      781 aaacaagtga catcaactct ttttcagcca tacatagaag aaatttgtga aagccttcga
      841 ggtgacattt ttcaaaaatt tatggaaagt gacaagttca ctagattttg tcagtggaaa
      901 aacgttgaat taaatatcca tttgaccatg aatgagttca gtgtgcatag gattattgga
      961 cgaggaggat tcggggaagt ttatggttgc aggaaagcag acactggaaa aatgtatgca
     1021 atgaaatgct tagataagaa gaggatcaaa atgaaacaag gagaaacatt agccttaaat
     1081 gaaagaatca tgttgtctct tgtcagcaca ggagactgtc ctttcattgt atgtatgacc
     1141 tatgccttcc ataccccaga taaactctgc ttcatcctgg atctgatgaa cgggggcgat
     1201 ttgcactacc acctttcaca acacggtgtg ttctctgaga aggagatgcg gttttatgcc
     1261 actgaaatca ttctgggtct ggaacacatg cacaatcggt ttgttgtcta cagagatttg
     1321 aagccagcaa atattctctt ggatgaacat ggacacgcaa gaatatcaga tcttggtctt
     1381 gcctgcgatt tttccaaaaa gaagcctcat gcgagtgttg gcacccatgg gtacatggct
     1441 cccgaggtgc tgcagaaggg gacggcctat gacagcagtg ccgactggtt ctccctgggc
     1501 tgcatgcttt tcaaacttct gagaggtcac agccctttca gacaacataa aaccaaagac
     1561 aagcatgaaa ttgaccgaat gacactcacc gtgaatgtgg aacttccaga caccttctct
     1621 cctgaactga agtccctttt ggagggcttg cttcagcgag acgttagcaa gcggctgggc
     1681 tgtcacggag gcggctcaca ggaagtaaaa gagcacagct ttttcaaagg tgttgactgg
     1741 cagcatgtct acttacaaaa gtacccacca cccttgattc ctccccgggg agaagtcaat
     1801 gctgctgatg cctttgatat tggctcattt gatgaagagg ataccaaagg gattaagcta
     1861 cttgattgcg accaagaact ctacaagaac ttccctttgg tcatctctga acgctggcag
     1921 caagaagtaa cggaaacagt ttatgaagca gtaaatgcag acacagataa aatcgaggcc
     1981 aggaagagag ctaaaaataa gcaacttggc cacgaagaag attacgctct ggggaaggac
     2041 tgtattatgc acgggtacat gctgaaactg ggaaacccat ttctgactca gtggcagcgt
     2101 cgctattttt acctctttcc aaatagactt gaatggagag gagagggaga gtcccggcaa
     2161 aatttactga caatggaaca gattctctct gtggaagaaa ctcaaattaa agacaaaaaa
     2221 tgcattttgt tcagaataaa aggagggaaa caatttgtct tgcaatgtga gagtgatcca
     2281 gagtttgtgc agtggaagaa agagttgaac gaaaccttca aggaggccca gcggctattg
     2341 cgtcgtgccc cgaagttcct caacaaacct cggtcaggta ctgtggagct cccaaagcca
     2401 tccctctgtc acagaaacag caacggcctc tagcacccag aaacagggag ggtcctcgag
     2461 gaggacacac cagggtctca gccttttggg gtgaacgagg atgaggcatc tgatctattc
     2521 gctaccggga ctcctccagg ctcccgagag gagtcgggac ccttcggctt ggggtcagct
     2581 cagctccctg ccttgtcaca tttgtctgca ttagaaacta ctgaagaaat aaaagttctt
     2641 tttctttgct acacactttg gtacctatga acctagaact tgaagtgact cctacttatc
     2701 acgtaaattt ttatgtctga tatcaaacac atcttagact ccccagaatg gaatttaaag
     2761 atgttcagtg ttgggtaaca gattgcccta agcattgcca catattctgt ctagtcactg
     2821 ctgattttct atgtctttgc tccatactgc tgggggatgg gagagccaca gtgtgtttct
     2881 tttgtgcact tcgcaactga cttcttgtcc tggggttaaa agttgaagat attttctgat
     2941 gatattaaaa gttgaagata tttctgcact tgggccctcc tctgggagcc gcacccacat
     3001 gactgccctg cctctgacca gtctgttccg gggccccctc agccaggtgg gaatgacgga
     3061 cacgtactat ccaagtgtat gggattaact aatcattgaa ggcattcatc cgtccatcat
     3121 tggaaagatt tacagtgatt ctgaaggaca ggccgtggag ttttaggttt caggggcaag
     3181 agcagttttc aaaagtcttt gagtccagtg tgcacgagtc gacaagcagt acctggcatg
     3241 caggagcact catgggtgag tccgtctcag gtctcgacaa ttagcagttg tgtgacagtc
     3301 attctggttc cttctgcctg accctgggag acatatcagt aatggatgta caaaagcagg
     3361 tctgttttat gtcttagtat aatttcagat gaattgtatt gaaaaaatgc tgaggaatga
     3421 atgtgtcaaa atgggttaac tgtgtatatt gactttcatg tcgtcatgca tctgtcatga
     3481 atgaatgata ctttgcactg ggctgtacga cagtgaggac cttagggcat gaagcctttt
     3541 tcctggtccc agcagcatct gccctgtgaa gtttgttttc tcccactgcc tccaggcccc
     3601 actgataccc ccaaatagat gctgggttat gagaaccagc gaaatccccc atgtcatcag
     3661 tcttaaaaaa aaaattttac aaatccacgt atttgtccca ttcttggagt agttttagtg
     3721 tatgtcttta cattaactac taacagtata aataacttga catcgtaatt gtctgcatcc
     3781 tgtccttgat atttttagca gttccaaatc tttgtttttg tatttgtttg ctgtgttcat
     3841 gggcaaagta agtacttttt aatgcagtta ttttgagagt ttggaagata attaccaaaa
     3901 gggtccatta tttcataaga gttactttgc aaaaaaaaaa atgtgggttt ttttttttgt
     3961 ctatctcaac tactagttgg ggtttaaatt aacatacatt ttctactatc tgttatttcc
     4021 agtgtgggag gagggatgta ctacttacat gcattctcct tatttaaaaa ggaagaatag
     4081 tattcaaatt ctgttgaaac acacacacac acacacacac acacacacac acactccaga
     4141 agcagaaaag ccattgttct taaagagtga atgtcttccc agccctggtt aattatagct
     4201 gtgactgatg ccgttcccgt ctgcatctca agctcatagg ttctcagcat gtgcagttga
     4261 ggatgcgctg ggcctcatgc ctgttctaga tctccaggat aaagggcctg ctgttgactc
     4321 caccagggtc tgggcttagc gtctaatatc tcgtacctag ggcgtgagct gcacaaacgt
     4381 gttcagaaag attattcaac tttcccatac ttgttctaaa attgagctga tccgcatctc
     4441 tttcaaaaac tagaatttct gctctaagaa tagaacataa ggctccactc ccttttagaa
     4501 aagatatatg aattggaaaa tgctctgaaa gtccttttgc ttcaaacaaa agtgtaaact
     4561 tttacacttc cccaactcac atttgatttg taatgatatg gttgagaagt acatctagat
     4621 gtcatttatt aaaagtgctt tgtaagacta gattgagctg tttctgaggg cggtcaccag
     4681 ttgtgttggg gtctggtttg agtgccttct gccaaaatgt tgtgatggag gtgtttctgc
     4741 gaccagacac aggataccgc tgtgtctgca cccggttgcc tgcatggcca gaggaaaagt
     4801 cagttggatt aaacatcatg gtatacttgg ctgttgtttt tttttaattt tttaattttt
     4861 tgggataggg cctcgctctg tcacccaggc tggagaacag tgggatgatc atggctcact
     4921 gcagccttga attcctaggt tcaagcaatc ctcccacgtc agcctcctga gtagctagga
     4981 ctacaggtgc atgccacctt tcctggctaa tttatttttt gggtagagat ggggtcttga
     5041 actcttaggc tcaagtgatc ctccttcctt ggcctcccaa aatgctggaa ttagagatgt
     5101 aagccaccat gcccagccat agtacttgga tgttttagaa ggttttccaa gtattacata
     5161 attcctagat gttcaccctt attacactcc aactattaaa aaggtcaaaa ttcagcctat
     5221 tttttttcat tattttagat tcctgtggtt gggatatttt aacattgatg agaaaaataa
     5281 ttgaggttga tatttttaca aaatcatgcg gtaataagtc ttgatttcat gattcaaaag
     5341 aatcaataaa gcctaaaaat aatagattac tttaagctgc tatgtaagat atatatggaa
     5401 taaattaaaa acctttgtga attcaggttt attattttta acctaaaaca ttctctttgg
     5461 ttcattcatc ccctcatgtc atgggggctc attggttttc cttctttgtc atatttaagt
     5521 atgatttttc aacaaaactt ctagaagtca gcttattatg tcaccattca tgcaaagtgc
     5581 tcatgcctct gattggtcca ttcactgacg tgacaatttc aggtcctatg tttaaaaaga
     5641 aggggctggc cgggcacgat ggctcacgcc tataatccca gcactttggg aggccgagag
     5701 gggcggttca cgaggtcagg agattgagac catcctggtt agcagagtga aaccccgtct
     5761 ctactaaaaa tacaaataaa aattagccgg gcgtggtggc gggcgcctgt agtcccagct
     5821 acttgggagg ctgaggcagg agaatggcat gaacccggga ggcagagctt gcagtgagcc
     5881 gagattgcgc cactgcactc cagcctgggc gacagagcga gactctgtct caaaaaaaaa
     5941 aaggaggggg gctaaatatc cagtgagatg cactgaggaa aggaagcatt ttgctgaaga
     6001 cagcagcagc aacaaacaat ggtctgtttg ttgcaaacaa gatgtagctt gatttctggt
     6061 ctgacatatg ccatatacag atattagaaa cgactgtttg aaggccacac tggtcatcta
     6121 caaagtaatg tttaccaatt gacgacaggg atttaactag attaaaaaga tcaaagtgtg
     6181 gtttttctct gctttttaaa atttcactcg gaatttgtag ctgggccaat tcaacacatt
     6241 ttacttttca gtggaattga tttttctaat gtttcagaat tttaacatat caagaagaaa
     6301 acaacgttct caaagtctgg cctctttagc atgatgtaaa cctatagaaa tgctttgaaa
     6361 tgtgctggtg taagataaga gttatcttgt atgatttaat catatgcagt gttgtctcag
     6421 ttacgttcag ggaaatgttt ctgtgtcatt cagagatgct tgatgaatta acacctccca
     6481 ccctgagtga ggggttgact tgttgggaga tgatttgggc ttcactggga tctgtgacag
     6541 gtgggggctg ggctgggtgt cacaaagaga atagtggtag aaatcgggcg aaggaagaaa
     6601 gaagttactg gtaaaaatca ttacaccata aagcaccaag gaaataactg agttaaaata
     6661 ggtgaagttt cttttttccc ccctgtaaca ggagagtttt ccttatgata attattctga
     6721 gacttggtca ctttgttttt gaatgtggag ctgctgaact cattcagaag ccatttgctg
     6781 cctatcagga ctttctgaag aagttctttt gcctctgcct accctctggc accctcccat
     6841 ggaggcacag gggacccaga gctaaagcat taccaggcca tctccaaaac accccgtgtg
     6901 tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg cactttgcag cccccgaggt
     6961 ggagaggcag tgtctggatc actgtgaatg cattgcccca ttggtcagtt ggggacactg
     7021 ttacaaatcc actgaagtcc tggtaaaact gtcaagagta acaggcctct tctgttctac
     7081 cctgctcact tccacggtga gttaccagcc tgggcaacac agcaagaccc catctctaca
     7141 aaaaaaattt ttttaagtaa ttaaccgttt aaattttttc ctaaagattt aacatgattt
     7201 ttccctccta tgtaaagttt actggagaga cttgaattac ttaaattcat gttaatatga
     7261 ttttttttta atccaggtca cattttaaca aagtttatta tgaaacaaat gaaatttgaa
     7321 ctctaaaatg gtactccttg gcttcctcaa gtcacaatga actttatatt ttctttgtcc
     7381 ttaaggacta agatagttgt tttatttcag ccgaatcaca gagataacca ctcctgcagg
     7441 cccccacagc tggcccaaag gggctgtctt tctgacctgg ctgtgttagc actgattgag
     7501 aaacgcaggc tcccaaattt taaattgcct ttattaaaaa cacaaactac agaaaatggg
     7561 ttaagagtat acgcatttca tcaaacacat ataggggaaa aaatccttca atttagagtt
     7621 aaataactca gctttgtata gtagagttag cgctccagta tctaacaatc tcagaatcat
     7681 ctctgaaaac tggtaactat gcttccattt ttaattttgt cctaaatatc agatgtcttt
     7741 gatgtaaggg tagggaatgg agaaatattt tcaattgtgt atttgtatta caaagaactt
     7801 gaaatttact ttcttagttg attatattaa atgatgtata tattatatgt ggtttataag
     7861 ctcaacactg gccatttttt tagttttatt gttaaatggt atttttctat gtttaattat
     7921 aatagatctg gctttttctg gatagcataa agatcactga actatatata tataagaaac
     7981 aagagttcta ttttagcaca aaggcatttt atattattta ttgaatccat aagtttgttt
     8041 tcgtcaaaaa cattccatat tatttctgct cctttttatt tgtatagttt gttatttaaa
     8101 gaaatggcag tccttcctgt tcttaataca ataaaattga aataatgcac ctagtaatgt
     8161 ggccgacatc tcttctcacc accatggact gttttcaaca acagttgatc ttctggtctg
     8221 tgctgagagg cgcatgcatg tctttcgtca cgtcgggcag cacacctgct gtgaaatact
     8281 gctttcatct acctcttcag aaggcttctt gcttgttgac aagtaccgca aaggctttat
     8341 tctggactgg ctatctcata aaaggatttc tgtaagactt tgcagtgtca ttccctcaga
     8401 acctaggttt gtttctaaag ccacggtatt gtccaggagc ccctgtgtgt ggggcaggta
     8461 gctatccctc ccatgtcatt agtaatcctt taggatttaa ggtacaactg gacagcatca
     8521 ttccttcccc ttattgtgcc aaatccccac catcagcctt gccattgcct taagatttga
     8581 ttattgcacc caattaccta accactaaac agaaaggcca ccttcactct ttgaaaaagg
     8641 caagctgtgc ttagaaacac tgcttttaag agtagcacat ttgagtgtga ctttttcccc
     8701 ccttcactat ttcaaaatgg ttttgaaatg gggtcttaaa ggtaagcgcc ctcatacatg
     8761 actgaaactt tgtgagaggt cttatatttg aatggaccct taatgattta tgtgaaatag
     8821 aatgaagtcc tgtctctgtg agagaacgtg cctcctcact catttgtctc tgtctgtttt
     8881 catagccatc aatatagtaa catatttact atattcttga atacccttga agaaagaaat
     8941 ccgttttcta ttgtgcattg ctatacgaag tgaagccagt aaactagata ctgtaaatct
     9001 agatattgta cctagacaaa atatcattgg ttctatctct ttttgtatct gttgtgccag
     9061 ggaaggttta taatcccttc tcagtataca ctcactagtg cacgtctgaa atagtatccc
     9121 acgggagatg ctgctccacg tctgaggtca cctgccctgt gtggggcaca ccaccgtcag
     9181 caccaccgtt tttacagtta ctttggagct gctagactgg ttttctgtgt tggtaaattg
     9241 cctatataaa tctgaataaa aaggatctgt acaaaga
//
