LOCUS       NM_001619               3403 bp    mRNA    linear   PRI 24-APR-2024
DEFINITION  Homo sapiens G protein-coupled receptor kinase 2 (GRK2), mRNA.
ACCESSION   NM_001619
VERSION     NM_001619.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3403)
  AUTHORS   Ye,P., Deng,Y., Gu,Y., Liu,P., Luo,J., Pu,J., Chen,J., Huang,Y.,
            Wang,N., Ji,Y. and Chen,S.
  TITLE     GRK2-YAP signaling is implicated in pulmonary arterial hypertension
            development
  JOURNAL   Chin Med J (Engl) 137 (7), 846-858 (2024)
   PUBMED   38242702
  REMARK    GeneRIF: GRK2-YAP signaling is implicated in pulmonary arterial
            hypertension development.
REFERENCE   2  (bases 1 to 3403)
  AUTHORS   Gao,M., Liang,X., Fan,M., Wu,Y., Dong,M.Y. and Du,R.L.
  TITLE     Exploring the potential biological function of GRK2 in colorectal
            cancer
  JOURNAL   Funct Integr Genomics 24 (2), 51 (2024)
   PUBMED   38446273
  REMARK    GeneRIF: Exploring the potential biological function of GRK2 in
            colorectal cancer.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3403)
  AUTHORS   Sanchez-Soto,M., Boldizsar,N.M., Schardien,K.A., Madaras,N.S.,
            Willette,B.K.A., Inbody,L.R., Dasaro,C., Moritz,A.E., Drube,J.,
            Haider,R.S., Free,R.B., Hoffman,C. and Sibley,D.R.
  TITLE     G Protein-Coupled Receptor Kinase 2 Selectively Enhances
            beta-Arrestin Recruitment to the D2 Dopamine Receptor through
            Mechanisms That Are Independent of Receptor Phosphorylation
  JOURNAL   Biomolecules 13 (10), 1552 (2023)
   PUBMED   37892234
  REMARK    GeneRIF: G Protein-Coupled Receptor Kinase 2 Selectively Enhances
            beta-Arrestin Recruitment to the D2 Dopamine Receptor through
            Mechanisms That Are Independent of Receptor Phosphorylation.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3403)
  AUTHORS   Zhang,J., Zhang,X., Shi,X., Liu,Y., Cheng,D., Tian,Q., Lin,N.,
            Wei,W. and Wu,H.
  TITLE     CXCL9, 10, 11/CXCR3 Axis Contributes to the Progress of Primary
            Sjogren's Syndrome by Activating GRK2 to Promote T Lymphocyte
            Migration
  JOURNAL   Inflammation 46 (3), 1047-1060 (2023)
   PUBMED   36801996
  REMARK    GeneRIF: CXCL9, 10, 11/CXCR3 Axis Contributes to the Progress of
            Primary Sjogren's Syndrome by Activating GRK2 to Promote T
            Lymphocyte Migration.
REFERENCE   5  (bases 1 to 3403)
  AUTHORS   Fu,L., Adu-Amankwaah,J., Sang,L., Tang,Z., Gong,Z., Zhang,X., Li,T.
            and Sun,H.
  TITLE     Gender differences in GRK2 in cardiovascular diseases and its
            interactions with estrogen
  JOURNAL   Am J Physiol Cell Physiol 324 (2), C505-C516 (2023)
   PUBMED   36622065
  REMARK    GeneRIF: Gender differences in GRK2 in cardiovascular diseases and
            its interactions with estrogen.
            Review article
REFERENCE   6  (bases 1 to 3403)
  AUTHORS   Guccione,M., Ettari,R., Taliani,S., Da Settimo,F., Zappala,M. and
            Grasso,S.
  TITLE     G-Protein-Coupled Receptor Kinase 2 (GRK2) Inhibitors: Current
            Trends and Future Perspectives
  JOURNAL   J Med Chem 59 (20), 9277-9294 (2016)
   PUBMED   27362616
  REMARK    Review article
REFERENCE   7  (bases 1 to 3403)
  AUTHORS   Eason,M.G., Moreira,S.P. and Liggett,S.B.
  TITLE     Four consecutive serines in the third intracellular loop are the
            sites for beta-adrenergic receptor kinase-mediated phosphorylation
            and desensitization of the alpha 2A-adrenergic receptor
  JOURNAL   J Biol Chem 270 (9), 4681-4688 (1995)
   PUBMED   7876239
REFERENCE   8  (bases 1 to 3403)
  AUTHORS   Prossnitz,E.R., Kim,C.M., Benovic,J.L. and Ye,R.D.
  TITLE     Phosphorylation of the N-formyl peptide receptor carboxyl terminus
            by the G protein-coupled receptor kinase, GRK2
  JOURNAL   J Biol Chem 270 (3), 1130-1137 (1995)
   PUBMED   7836371
REFERENCE   9  (bases 1 to 3403)
  AUTHORS   Chuang,T.T., Sallese,M., Ambrosini,G., Parruti,G. and De Blasi,A.
  TITLE     High expression of beta-adrenergic receptor kinase in human
            peripheral blood leukocytes. Isoproterenol and platelet activating
            factor can induce kinase translocation
  JOURNAL   J Biol Chem 267 (10), 6886-6892 (1992)
   PUBMED   1339451
REFERENCE   10 (bases 1 to 3403)
  AUTHORS   Benovic,J.L., Stone,W.C., Huebner,K., Croce,C., Caron,M.G. and
            Lefkowitz,R.J.
  TITLE     cDNA cloning and chromosomal localization of the human
            beta-adrenergic receptor kinase
  JOURNAL   FEBS Lett 283 (1), 122-126 (1991)
   PUBMED   2037065
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC090863.1.
            
            On Nov 22, 2018 this sequence version replaced NM_001619.4.
            
            Summary: This gene encodes a member of the G protein-coupled
            receptor kinase family of proteins. The encoded protein
            phosphorylates the beta-adrenergic receptor as well as a wide range
            of other substrates including non-GPCR cell surface receptors, and
            cytoskeletal, mitochondrial, and transcription factor proteins.
            Data from rodent models supports a role for this gene in embryonic
            development, heart function and metabolism. Elevated expression of
            this gene has been observed in human patients with heart failure
            and Alzheimer's disease. [provided by RefSeq, Sep 2017].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC037963.1, SRR1163657.418571.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000308595.10/ ENSP00000312262.5
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-204               BC090863.1         5-208
            205-3403            BC090863.1         212-3410
FEATURES             Location/Qualifiers
     source          1..3403
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11q13.2"
     gene            1..3403
                     /gene="GRK2"
                     /gene_synonym="ADRBK1; BARK1; BETA-ARK1"
                     /note="G protein-coupled receptor kinase 2"
                     /db_xref="GeneID:156"
                     /db_xref="HGNC:HGNC:289"
                     /db_xref="MIM:109635"
     exon            1..340
                     /gene="GRK2"
                     /gene_synonym="ADRBK1; BARK1; BETA-ARK1"
                     /inference="alignment:Splign:2.1.0"
     CDS             228..2297
                     /gene="GRK2"
                     /gene_synonym="ADRBK1; BARK1; BETA-ARK1"
                     /EC_number="2.7.11.15"
                     /note="beta-ARK-1; adrenergic beta receptor kinase 1"
                     /codon_start=1
                     /product="beta-adrenergic receptor kinase 1"
                     /protein_id="NP_001610.2"
                     /db_xref="CCDS:CCDS8156.1"
                     /db_xref="GeneID:156"
                     /db_xref="HGNC:HGNC:289"
                     /db_xref="MIM:109635"
                     /translation="MADLEAVLADVSYLMAMEKSKATPAARASKKILLPEPSIRSVMQ
                     KYLEDRGEVTFEKIFSQKLGYLLFRDFCLNHLEEARPLVEFYEEIKKYEKLETEEERV
                     ARSREIFDSYIMKELLACSHPFSKSATEHVQGHLGKKQVPPDLFQPYIEEICQNLRGD
                     VFQKFIESDKFTRFCQWKNVELNIHLTMNDFSVHRIIGRGGFGEVYGCRKADTGKMYA
                     MKCLDKKRIKMKQGETLALNERIMLSLVSTGDCPFIVCMSYAFHTPDKLSFILDLMNG
                     GDLHYHLSQHGVFSEADMRFYAAEIILGLEHMHNRFVVYRDLKPANILLDEHGHVRIS
                     DLGLACDFSKKKPHASVGTHGYMAPEVLQKGVAYDSSADWFSLGCMLFKLLRGHSPFR
                     QHKTKDKHEIDRMTLTMAVELPDSFSPELRSLLEGLLQRDVNRRLGCLGRGAQEVKES
                     PFFRSLDWQMVFLQKYPPPLIPPRGEVNAADAFDIGSFDEEDTKGIKLLDSDQELYRN
                     FPLTISERWQQEVAETVFDTINAETDRLEARKKAKNKQLGHEEDYALGKDCIMHGYMS
                     KMGNPFLTQWQRRYFYLFPNRLEWRGEGEAPQSLLTMEEIQSVEETQIKERKCLLLKI
                     RGGKQFILQCDSDPELVQWKKELRDAYREAQQLVQRVPKMKNKPRSPVVELSKVPLVQ
                     RGSANGL"
     exon            341..417
                     /gene="GRK2"
                     /gene_synonym="ADRBK1; BARK1; BETA-ARK1"
                     /inference="alignment:Splign:2.1.0"
     exon            418..491
                     /gene="GRK2"
                     /gene_synonym="ADRBK1; BARK1; BETA-ARK1"
                     /inference="alignment:Splign:2.1.0"
     exon            492..593
                     /gene="GRK2"
                     /gene_synonym="ADRBK1; BARK1; BETA-ARK1"
                     /inference="alignment:Splign:2.1.0"
     exon            594..668
                     /gene="GRK2"
                     /gene_synonym="ADRBK1; BARK1; BETA-ARK1"
                     /inference="alignment:Splign:2.1.0"
     exon            669..730
                     /gene="GRK2"
                     /gene_synonym="ADRBK1; BARK1; BETA-ARK1"
                     /inference="alignment:Splign:2.1.0"
     exon            731..782
                     /gene="GRK2"
                     /gene_synonym="ADRBK1; BARK1; BETA-ARK1"
                     /inference="alignment:Splign:2.1.0"
     exon            783..874
                     /gene="GRK2"
                     /gene_synonym="ADRBK1; BARK1; BETA-ARK1"
                     /inference="alignment:Splign:2.1.0"
     exon            875..974
                     /gene="GRK2"
                     /gene_synonym="ADRBK1; BARK1; BETA-ARK1"
                     /inference="alignment:Splign:2.1.0"
     exon            975..1053
                     /gene="GRK2"
                     /gene_synonym="ADRBK1; BARK1; BETA-ARK1"
                     /inference="alignment:Splign:2.1.0"
     exon            1054..1184
                     /gene="GRK2"
                     /gene_synonym="ADRBK1; BARK1; BETA-ARK1"
                     /inference="alignment:Splign:2.1.0"
     exon            1185..1279
                     /gene="GRK2"
                     /gene_synonym="ADRBK1; BARK1; BETA-ARK1"
                     /inference="alignment:Splign:2.1.0"
     exon            1280..1387
                     /gene="GRK2"
                     /gene_synonym="ADRBK1; BARK1; BETA-ARK1"
                     /inference="alignment:Splign:2.1.0"
     exon            1388..1454
                     /gene="GRK2"
                     /gene_synonym="ADRBK1; BARK1; BETA-ARK1"
                     /inference="alignment:Splign:2.1.0"
     exon            1455..1555
                     /gene="GRK2"
                     /gene_synonym="ADRBK1; BARK1; BETA-ARK1"
                     /inference="alignment:Splign:2.1.0"
     exon            1556..1622
                     /gene="GRK2"
                     /gene_synonym="ADRBK1; BARK1; BETA-ARK1"
                     /inference="alignment:Splign:2.1.0"
     exon            1623..1718
                     /gene="GRK2"
                     /gene_synonym="ADRBK1; BARK1; BETA-ARK1"
                     /inference="alignment:Splign:2.1.0"
     exon            1719..1881
                     /gene="GRK2"
                     /gene_synonym="ADRBK1; BARK1; BETA-ARK1"
                     /inference="alignment:Splign:2.1.0"
     exon            1882..2018
                     /gene="GRK2"
                     /gene_synonym="ADRBK1; BARK1; BETA-ARK1"
                     /inference="alignment:Splign:2.1.0"
     exon            2019..2132
                     /gene="GRK2"
                     /gene_synonym="ADRBK1; BARK1; BETA-ARK1"
                     /inference="alignment:Splign:2.1.0"
     exon            2133..3403
                     /gene="GRK2"
                     /gene_synonym="ADRBK1; BARK1; BETA-ARK1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3385..3390
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GRK2"
                     /gene_synonym="ADRBK1; BARK1; BETA-ARK1"
                     /note="hexamer: TATAAA"
     polyA_site      3403
                     /gene="GRK2"
                     /gene_synonym="ADRBK1; BARK1; BETA-ARK1"
                     /note="major polyA site"
ORIGIN      
        1 agtcccggcg ggagcggagc gcgagccggg gccgggcccg agccggcgcc atggggcggc
       61 gccgcctgtg agcggcggcg agcggagccg cgggcgccga gcagggccag gcgggaggcg
      121 tcggcgcccg aggccgagcg agccgcggcc gggccgggcc gagcgccgag cgagcaggag
      181 cggcggcggc ggcggcggcg gcgggaggag gcagcgccgc cgccaagatg gcggacctgg
      241 aggcggtgct ggccgacgtg agctacctga tggccatgga gaagagcaag gccacgccgg
      301 ccgcgcgcgc cagcaagaag atcctgctgc ccgagcccag catccgcagt gtcatgcaga
      361 agtacctgga ggaccggggc gaggtgacct ttgagaagat cttttcccag aagctggggt
      421 acctgctctt ccgagacttc tgcctgaacc acctggagga ggccaggccc ttggtggaat
      481 tctatgagga gatcaagaag tacgagaagc tggagacgga ggaggagcgt gtggcccgca
      541 gccgggagat cttcgactca tacatcatga aggagctgct ggcctgctcg catcccttct
      601 cgaagagtgc cactgagcat gtccaaggcc acctggggaa gaagcaggtg cctccggatc
      661 tcttccagcc atacatcgaa gagatttgtc aaaacctccg aggggacgtg ttccagaaat
      721 tcattgagag cgataagttc acacggtttt gccagtggaa gaatgtggag ctcaacatcc
      781 acctgaccat gaatgacttc agcgtgcatc gcatcattgg gcgcgggggc tttggcgagg
      841 tctatgggtg ccggaaggct gacacaggca agatgtacgc catgaagtgc ctggacaaaa
      901 agcgcatcaa gatgaagcag ggggagaccc tggccctgaa cgagcgcatc atgctctcgc
      961 tcgtcagcac tggggactgc ccattcattg tctgcatgtc atacgcgttc cacacgccag
     1021 acaagctcag cttcatcctg gacctcatga acggtgggga cctgcactac cacctctccc
     1081 agcacggggt cttctcagag gctgacatgc gcttctatgc ggccgagatc atcctgggcc
     1141 tggagcacat gcacaaccgc ttcgtggtct accgggacct gaagccagcc aacatccttc
     1201 tggacgagca tggccacgtg cggatctcgg acctgggcct ggcctgtgac ttctccaaga
     1261 agaagcccca tgccagcgtg ggcacccacg ggtacatggc tccggaggtc ctgcagaagg
     1321 gcgtggccta cgacagcagt gccgactggt tctctctggg gtgcatgctc ttcaagttgc
     1381 tgcgggggca cagccccttc cggcagcaca agaccaaaga caagcatgag atcgaccgca
     1441 tgacgctgac gatggccgtg gagctgcccg actccttctc ccctgaacta cgctccctgc
     1501 tggaggggtt gctgcagagg gatgtcaacc ggagattggg ctgcctgggc cgaggggctc
     1561 aggaggtgaa agagagcccc tttttccgct ccctggactg gcagatggtc ttcttgcaga
     1621 agtaccctcc cccgctgatc cccccacgag gggaggtgaa cgcggccgac gccttcgaca
     1681 ttggctcctt cgatgaggag gacacaaaag gaatcaagtt actggacagt gatcaggagc
     1741 tctaccgcaa cttccccctc accatctcgg agcggtggca gcaggaggtg gcagagactg
     1801 tcttcgacac catcaacgct gagacagacc ggctggaggc tcgcaagaaa gccaagaaca
     1861 agcagctggg ccatgaggaa gactacgccc tgggcaagga ctgcatcatg catggctaca
     1921 tgtccaagat gggcaacccc ttcctgaccc agtggcagcg gcggtacttc tacctgttcc
     1981 ccaaccgcct cgagtggcgg ggcgagggcg aggccccgca gagcctgctg accatggagg
     2041 agatccagtc ggtggaggag acgcagatca aggagcgcaa gtgcctgctc ctcaagatcc
     2101 gcggtgggaa acagttcatt ttgcagtgcg atagcgaccc tgagctggtg cagtggaaga
     2161 aggagctgcg cgacgcctac cgcgaggccc agcagctggt gcagcgggtg cccaagatga
     2221 agaacaagcc gcgctcgccc gtggtggagc tgagcaaggt gccgctggtc cagcgcggca
     2281 gtgccaacgg cctctgaccc gcccacccgc cttttataaa cctctaattt attttgtcga
     2341 atttttatta tttgttttcc cgccaagcgg aaaaggtttt attttgtaat tattgtgatt
     2401 tcccgtggcc ccagcctggc ccagctcccc cgggaggggc ccgcttgcct cggctcctgc
     2461 tgcaccaacc cagccgctgc ccggcgccct ctgtcctgac ttcaggggct gcccgctccc
     2521 agtgtcttcc tgtgggggaa gagcacagcc ctcccgcccc ttccccgagg gatgatgcca
     2581 caccaagctg tgccaccctg ggctctgtgg gctgcactct gtgcccatgg gcactgctgg
     2641 gtggcccatc ccccctcacc aggggcaggc acagcacagg gatccgactt gaattttccc
     2701 actgcacccc ctcctgctgc agaggggcag gccctgcact gtcctgctcc acagtgttgg
     2761 cgagaggagg ggcccgttgt ctccctggcc ctcaaggcct cccacagtga ctcgggctcc
     2821 tgtgccctta ttcaggaaaa gcctctgtgt cactggctgc ctccactccc acttccctga
     2881 cactgcgggg cttggctgag agagtggcat tggcagcagg tgctgctacc ctccctgctg
     2941 tcccctcttg ccccaacccc cagcacccgg gctcagggac cacagcaagg cacctgcagg
     3001 ttgggccata ctggcctcgc ctggcctgag gtctcgctga tgctgggctg ggtgcgaccc
     3061 catctgccca ggacggggcc ggccaggtgg gcgggcagca cagcaaggag gctggctggg
     3121 gcctatcagt gtgcccccca tcctggccca tcagtgtacc cccgcccagg ctggccagcc
     3181 ccacagccca cgtcctgtca gtgccgccgc ctcgcccacc gcatgccccc tcgtgccagt
     3241 cgcgctgcct gtgtggtgtc gcgccttctc ccccccgggg ctgggttggc gcaccctccc
     3301 ctcccgtcta ctcattcccc ggggcgtttc tttgccgatt tttgaatgtg attttaaaga
     3361 gtgaaaaatg agactatgcg tttttataaa aaatggtgcc tga
//
