LOCUS       NM_001366722            5147 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens glutamate receptor interacting protein 1 (GRIP1),
            transcript variant 3, mRNA.
ACCESSION   NM_001366722 XM_011538093
VERSION     NM_001366722.1
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5147)
  AUTHORS   Segal,D., Maier,S., Mastromarco,G.J., Qian,W.W., Nabeel-Shah,S.,
            Lee,H., Moore,G., Lacoste,J., Larsen,B., Lin,Z.Y.,
            Selvabaskaran,A., Liu,K., Smibert,C., Zhang,Z., Greenblatt,J.,
            Peng,J., Lee,H.O., Gingras,A.C. and Taipale,M.
  TITLE     A central chaperone-like role for 14-3-3 proteins in human cells
  JOURNAL   Mol Cell 83 (6), 974-993 (2023)
   PUBMED   36931259
REFERENCE   2  (bases 1 to 5147)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   3  (bases 1 to 5147)
  AUTHORS   Koprulu,M., Kumare,A., Bibi,A., Malik,S. and Tolun,A.
  TITLE     The first adolescent case of Fraser syndrome 3, with a novel
            nonsense variant in GRIP1
  JOURNAL   Am J Med Genet A 185 (6), 1858-1863 (2021)
   PUBMED   33709629
REFERENCE   4  (bases 1 to 5147)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   5  (bases 1 to 5147)
  AUTHORS   Twelvetrees,A.E., Lesept,F., Holzbaur,E.L.F. and Kittler,J.T.
  TITLE     The adaptor proteins HAP1a and GRIP1 collaborate to activate the
            kinesin-1 isoform KIF5C
  JOURNAL   J Cell Sci 132 (24) (2019)
   PUBMED   31757889
  REMARK    GeneRIF: The adaptor proteins HAP1a and GRIP1 collaborate to
            activate the kinesin-1 isoform KIF5C.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 5147)
  AUTHORS   Lu,W. and Ziff,E.B.
  TITLE     PICK1 interacts with ABP/GRIP to regulate AMPA receptor trafficking
  JOURNAL   Neuron 47 (3), 407-421 (2005)
   PUBMED   16055064
REFERENCE   7  (bases 1 to 5147)
  AUTHORS   Charych,E.I., Yu,W., Li,R., Serwanski,D.R., Miralles,C.P., Li,X.,
            Yang,B.Y., Pinal,N., Walikonis,R. and De Blas,A.L.
  TITLE     A four PDZ domain-containing splice variant form of GRIP1 is
            localized in GABAergic and glutamatergic synapses in the brain
  JOURNAL   J Biol Chem 279 (37), 38978-38990 (2004)
   PUBMED   15226318
  REMARK    GeneRIF: GRIP1c 4-7 plays a role not only in glutamatergic synapses
            but also in GABAergic synapses
REFERENCE   8  (bases 1 to 5147)
  AUTHORS   Braithwaite,S.P., Xia,H. and Malenka,R.C.
  TITLE     Differential roles for NSF and GRIP/ABP in AMPA receptor cycling
  JOURNAL   Proc Natl Acad Sci U S A 99 (10), 7096-7101 (2002)
   PUBMED   12011465
  REMARK    GeneRIF: after regulated endocytosis, binding to GRIP/ABP
            stabilizes the internalized receptors in an intracellular pool and
            prevents them from being recycled back to the plasma membrane or
            entering a degradative pathway.
REFERENCE   9  (bases 1 to 5147)
  AUTHORS   Lin,S.H., Arai,A.C., Wang,Z., Nothacker,H.P. and Civelli,O.
  TITLE     The carboxyl terminus of the prolactin-releasing peptide receptor
            interacts with PDZ domain proteins involved in
            alpha-amino-3-hydroxy-5-methylisoxazole-4-propionic acid receptor
            clustering
  JOURNAL   Mol Pharmacol 60 (5), 916-923 (2001)
   PUBMED   11641419
REFERENCE   10 (bases 1 to 5147)
  AUTHORS   Bruckner,K., Pablo Labrador,J., Scheiffele,P., Herb,A.,
            Seeburg,P.H. and Klein,R.
  TITLE     EphrinB ligands recruit GRIP family PDZ adaptor proteins into raft
            membrane microdomains
  JOURNAL   Neuron 22 (3), 511-524 (1999)
   PUBMED   10197531
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC122686.6, AC090710.16,
            AC135251.2 and AC078889.20.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Oct 17, 2018 this sequence version replaced XM_011538093.2.
            
            Summary: This gene encodes a member of the glutamate receptor
            interacting protein family. The encoded scaffold protein binds to
            and mediates the trafficking and membrane organization of a number
            of transmembrane proteins. Alternatively spliced transcript
            variants encoding different isoforms have been described. [provided
            by RefSeq, May 2010].
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: mixed sample support SAMEA1965299, SAMEA1966682
                              [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            inferred exon combination :: based on alignments, homology
            MANE Ensembl match        :: ENST00000359742.9/ ENSP00000352780.4
            RefSeq Select criteria    :: based on manual assertion,
                                         conservation
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-227               AC122686.6         52734-52960
            228-308             AC090710.16        159651-159731       c
            309-444             AC090710.16        104619-104754       c
            445-590             AC090710.16        101882-102027       c
            591-674             AC090710.16        92635-92718         c
            675-750             AC090710.16        80705-80780         c
            751-896             AC090710.16        78423-78568         c
            897-1044            AC090710.16        28079-28226         c
            1045-1214           AC090710.16        25728-25897         c
            1215-1370           AC090710.16        18991-19146         c
            1371-1526           AC090710.16        18213-18368         c
            1527-1713           AC090710.16        8126-8312           c
            1714-1859           AC090710.16        7388-7533           c
            1860-1940           AC135251.2         6565-6645
            1941-2010           AC135251.2         18404-18473
            2011-2156           AC135251.2         32765-32910
            2157-2301           AC135251.2         44841-44985
            2302-2441           AC135251.2         46377-46516
            2442-2636           AC135251.2         46691-46885
            2637-2793           AC135251.2         59757-59913
            2794-2905           AC135251.2         61908-62019
            2906-2950           AC135251.2         62132-62176
            2951-3184           AC135251.2         67266-67499
            3185-3331           AC078889.20        17248-17394
            3332-5147           AC078889.20        21565-23380
FEATURES             Location/Qualifiers
     source          1..5147
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12q14.3"
     gene            1..5147
                     /gene="GRIP1"
                     /gene_synonym="FRASRS3; GRIP"
                     /note="glutamate receptor interacting protein 1"
                     /db_xref="GeneID:23426"
                     /db_xref="HGNC:HGNC:18708"
                     /db_xref="MIM:604597"
     exon            1..227
                     /gene="GRIP1"
                     /gene_synonym="FRASRS3; GRIP"
                     /inference="alignment:Splign:2.1.0"
     CDS             173..3559
                     /gene="GRIP1"
                     /gene_synonym="FRASRS3; GRIP"
                     /note="isoform 3 is encoded by transcript variant 3"
                     /codon_start=1
                     /product="glutamate receptor-interacting protein 1 isoform
                     3"
                     /protein_id="NP_001353651.1"
                     /db_xref="CCDS:CCDS91722.1"
                     /db_xref="GeneID:23426"
                     /db_xref="HGNC:HGNC:18708"
                     /db_xref="MIM:604597"
                     /translation="MIAVSFKCRCQILRRLTKDESPYTKSASQTKPPDGALAVRRQSI
                     PEEFKGSTVVELMKKEGTTLGLTVSGGIDKDGKPRVSNLRQGGIAARSDQLDVGDYIK
                     AVNGINLAKFRHDEIISLLKNVGERVVLEVEYELPPVSVQGSSVIFRTVEVTLHKEGN
                     TFGFVIRGGAHDDRNKSRPVVITCVRPGGPADREGTIKPGDRLLSVDGIRLLGTTHAE
                     AMSILKQCGQEAALLIEYDVSVMDSVATASGPLLVEVAKTPGASLGVALTTSMCCNKQ
                     VIVIDKIKSASIADRCGALHVGDHILSIDGTSMEYCTLAEATQFLANTTDQVKLEILP
                     HHQTRLALKGPDHVKIQRSDRQLTWDSWASNHSSLHTNHHYNTYHPDHCRVPALTFPK
                     APPPNSPPALVSSSFSPTSMSAYSLSSLNMGTLPRSLYSTSPRGTMMRRRLKKKDFKS
                     SLSLASSTVGLAGQVVHTETTEVVLTADPVTGFGIQLQGSVFATETLSSPPLISYIEA
                     DSPAERCGVLQIGDRVMAINGIPTEDSTFEEASQLLRDSSITSKVTLEIEFDVAESVI
                     PSSGTFHVKLPKKHNVELGITISSPSSRKPGDPLVISDIKKGSVAHRTGTLELGDKLL
                     AIDNIRLDNCSMEDAVQILQQCEDLVKLKIRKDEDNSDEQESSGAIIYTVELKRYGGP
                     LGITISGTEEPFDPIIISSLTKGGLAERTGAIHIGDRILAINSSSLKGKPLSEAIHLL
                     QMAGETVTLKIKKQTDAQSASSPKKFPISSHLSDLGDVEEDSSPAQKPGKLSDMYPST
                     VPSVDSAVDSWDGSAIDTSYGTQGTSFQASGYNFNTYDWRSPKQRGSLSPVTKPRSQT
                     YPDVGLSYEDWDRSTASGFAGAADSAETEQEENFWSQALEDLETCGQSGILRELEEKA
                     DRRVSLRNMTLLATIMSGSTMSLNHEAPTPRSQLGRQASFQERSSSRPHYSQTTRSNT
                     LPSDVGRKSVTLRKMKQEIKEIMSPTPVELHKVTLYKDSDMEDFGFSVADGLLEKGVY
                     VKNIRPAGPGDLGGLKPYDRLLQVNHVRTRDFDCCLVVPLIAESGNKLDLVISRNPLA
                     SQKSIDQQSLPGDWSEQNSAFFQQPSHGGNLETREPTNTL"
     exon            228..308
                     /gene="GRIP1"
                     /gene_synonym="FRASRS3; GRIP"
                     /inference="alignment:Splign:2.1.0"
     exon            309..444
                     /gene="GRIP1"
                     /gene_synonym="FRASRS3; GRIP"
                     /inference="alignment:Splign:2.1.0"
     exon            445..590
                     /gene="GRIP1"
                     /gene_synonym="FRASRS3; GRIP"
                     /inference="alignment:Splign:2.1.0"
     exon            591..674
                     /gene="GRIP1"
                     /gene_synonym="FRASRS3; GRIP"
                     /inference="alignment:Splign:2.1.0"
     exon            675..750
                     /gene="GRIP1"
                     /gene_synonym="FRASRS3; GRIP"
                     /inference="alignment:Splign:2.1.0"
     exon            751..896
                     /gene="GRIP1"
                     /gene_synonym="FRASRS3; GRIP"
                     /inference="alignment:Splign:2.1.0"
     exon            897..1044
                     /gene="GRIP1"
                     /gene_synonym="FRASRS3; GRIP"
                     /inference="alignment:Splign:2.1.0"
     exon            1045..1214
                     /gene="GRIP1"
                     /gene_synonym="FRASRS3; GRIP"
                     /inference="alignment:Splign:2.1.0"
     exon            1215..1370
                     /gene="GRIP1"
                     /gene_synonym="FRASRS3; GRIP"
                     /inference="alignment:Splign:2.1.0"
     exon            1371..1526
                     /gene="GRIP1"
                     /gene_synonym="FRASRS3; GRIP"
                     /inference="alignment:Splign:2.1.0"
     exon            1527..1713
                     /gene="GRIP1"
                     /gene_synonym="FRASRS3; GRIP"
                     /inference="alignment:Splign:2.1.0"
     exon            1714..1859
                     /gene="GRIP1"
                     /gene_synonym="FRASRS3; GRIP"
                     /inference="alignment:Splign:2.1.0"
     exon            1860..1940
                     /gene="GRIP1"
                     /gene_synonym="FRASRS3; GRIP"
                     /inference="alignment:Splign:2.1.0"
     exon            1941..2010
                     /gene="GRIP1"
                     /gene_synonym="FRASRS3; GRIP"
                     /inference="alignment:Splign:2.1.0"
     exon            2011..2156
                     /gene="GRIP1"
                     /gene_synonym="FRASRS3; GRIP"
                     /inference="alignment:Splign:2.1.0"
     exon            2157..2301
                     /gene="GRIP1"
                     /gene_synonym="FRASRS3; GRIP"
                     /inference="alignment:Splign:2.1.0"
     exon            2302..2441
                     /gene="GRIP1"
                     /gene_synonym="FRASRS3; GRIP"
                     /inference="alignment:Splign:2.1.0"
     exon            2442..2636
                     /gene="GRIP1"
                     /gene_synonym="FRASRS3; GRIP"
                     /inference="alignment:Splign:2.1.0"
     exon            2637..2793
                     /gene="GRIP1"
                     /gene_synonym="FRASRS3; GRIP"
                     /inference="alignment:Splign:2.1.0"
     exon            2794..2905
                     /gene="GRIP1"
                     /gene_synonym="FRASRS3; GRIP"
                     /inference="alignment:Splign:2.1.0"
     exon            2906..2950
                     /gene="GRIP1"
                     /gene_synonym="FRASRS3; GRIP"
                     /inference="alignment:Splign:2.1.0"
     exon            2951..3184
                     /gene="GRIP1"
                     /gene_synonym="FRASRS3; GRIP"
                     /inference="alignment:Splign:2.1.0"
     exon            3185..3331
                     /gene="GRIP1"
                     /gene_synonym="FRASRS3; GRIP"
                     /inference="alignment:Splign:2.1.0"
     exon            3332..5147
                     /gene="GRIP1"
                     /gene_synonym="FRASRS3; GRIP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5103..5108
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GRIP1"
                     /gene_synonym="FRASRS3; GRIP"
                     /note="hexamer: AATAAA"
     polyA_site      5147
                     /gene="GRIP1"
                     /gene_synonym="FRASRS3; GRIP"
                     /note="major polyA site"
ORIGIN      
        1 agatgacatg tagcggacct ctgcatcagc caagaggaat taagctttgt cactccccac
       61 tgggactacc tttctcctgg tatgtgcgca aggaattcat atgctgctgc tgctgcagcc
      121 accagagcag agagccttga gtacactttg ccacagaaag cagtgagcaa gaatgatagc
      181 tgtctctttt aaatgccgtt gtcaaattct gaggcgactt actaaagatg agagtcccta
      241 cactaaatcc gccagccaga caaagccgcc tgatggagcg ttggctgtga ggagacagag
      301 catcccagag gaattcaagg gctccacagt cgtcgagctg atgaagaagg aaggcactac
      361 cctgggtctg acggtatcgg gaggaattga taaggatggc aagccaagag tatctaatct
      421 gcggcaagga ggaattgctg ctagaagtga ccagctggat gtgggtgact acatcaaagc
      481 agtgaatgga atcaacctgg ccaaattccg ccatgacgag atcatcagct tgctgaagaa
      541 tgtgggagaa agagtggttc ttgaagtaga gtacgagctt ccaccggtct ctgtgcaagg
      601 atcaagtgtt attttccgaa cagtggaggt cacattacat aaagaaggca atacctttgg
      661 ttttgtaatt cgagggggag cacatgatga tagaaataaa tctcgtccag ttgtgataac
      721 atgtgttcgt cctggagggc ctgctgacag agagggcacg atcaaacccg gtgacaggtt
      781 gctcagtgtg gatggaattc ggcttcttgg aactacgcat gctgaagcca tgagtattct
      841 taaacaatgt ggacaagaag cagcactgct gatagaatat gatgtctcag taatggactc
      901 tgtggcaaca gcatccgggc cactactagt cgaagttgcc aaaactcctg gtgccagcct
      961 tggggttgcc ctaactacct cgatgtgctg taacaaacaa gtcattgtca tagacaaaat
     1021 caaatctgca agtattgcag acagatgtgg cgcattgcat gtgggagatc acatcctctc
     1081 catcgatgga accagcatgg agtactgtac acttgcagaa gcaacccagt tcctggccaa
     1141 caccactgac caggtcaagc ttgagatcct tccccatcat cagacccggc tggccctaaa
     1201 ggggcccgac catgtgaaaa ttcagaggag cgacaggcaa cttacctggg attcctgggc
     1261 cagcaaccac agcagccttc acaccaacca tcactataac acgtaccacc ctgaccattg
     1321 cagagtacca gccctgacat tcccgaaagc acctcctcca aacagccctc cagctttggt
     1381 gtcttcatcc ttctctccta cctccatgag tgcatacagc ctgagttccc tgaacatggg
     1441 gactctacct cgaagcctct actccaccag cccacgtgga accatgatga ggaggagact
     1501 gaaaaagaaa gacttcaaaa gctcattgtc cttagcctcc agcacagtag gattggctgg
     1561 gcaggttgtt cacacagaaa ccacagaggt tgtgctgacg gcagatcctg tcacaggatt
     1621 tgggatccaa ctgcagggca gtgtgtttgc cacagaaact ctctcttctc cacctctgat
     1681 ttcctatatc gaagctgaca gcccagcaga gagatgtggg gtgctacaga ttggagacag
     1741 agtgatggcc atcaatggaa ttccaacaga agacagcacc ttcgaagaag ccagtcagct
     1801 cctccgagac tcttcaatca cgagcaaggt cacactggaa atcgagtttg atgttgcaga
     1861 gtctgtcatc ccaagtagtg gaacatttca tgtaaagctg cctaagaagc acaatgtgga
     1921 acttggaata accataagtt caccatccag tagaaaacca ggagaccccc tcgtcatttc
     1981 agatatcaag aaagggagtg tggcacacag aactgggacc ctggaacttg gggataaatt
     2041 gctcgcaata gataatatcc ggctggacaa ctgttccatg gaagatgcag ttcagatcct
     2101 ccagcaatgt gaagacctgg tgaagctcaa aatccgcaaa gatgaagata attcagatga
     2161 gcaagaaagt tccggagcaa ttatttacac cgtggagctt aaacgctacg gggggcccct
     2221 tggcatcaca atttcaggaa ctgaagagcc gtttgatcct ataatcattt caagcctcac
     2281 taaaggggga ttagctgaaa gaactggcgc aatccacata ggagaccgaa tcctagccat
     2341 caatagcagc agcttgaaag ggaagcctct gagtgaagcc atccatttgt tacagatggc
     2401 aggagagact gtcaccttga aaattaagaa acagacagat gcccagtcag catcgagccc
     2461 caagaagttc cctatttcta gccatttgag tgacctgggg gatgtggagg aggactcctc
     2521 accagcacag aagccaggca agctctccga catgtacccc tccacggtgc ccagtgtgga
     2581 cagtgctgtg gattcatggg atgggtctgc aatagacacc agctatggaa ctcaaggcac
     2641 tagttttcag gcctcaggat acaatttcaa cacctatgac tggaggagtc caaaacagag
     2701 aggcagcttg tccccagtca ctaagcctcg aagccagact tacccagatg tggggctgag
     2761 ttatgaagac tgggaccggt ccacagccag tggttttgca ggggctgccg atagtgcaga
     2821 gacagaacaa gaggagaact tctggtctca agcgctggag gatttggaaa cctgcggaca
     2881 gtcaggaatt ctgagagaac tggaggagaa agctgacagg cgtgtgtctt tgagaaacat
     2941 gactctcttg gcaacaatca tgtcggggag cacgatgagt ttgaatcatg aggctccaac
     3001 acctcgcagt cagctggggc gacaggccag cttccaggag cgcagcagct cgcggccgca
     3061 ctacagccaa acaactcgga gcaacaccct gccttcagat gtgggtagga agtcagtaac
     3121 cctgagaaaa atgaaacaag aaataaagga gatcatgtct ccaactcctg tggagctgca
     3181 caaggtgacc ttgtacaagg actctgacat ggaggacttt gggttcagtg tagcagatgg
     3241 cttactggag aaaggagtgt atgtcaaaaa tattcgccca gctgggccag gagatcttgg
     3301 tggcttaaag ccctatgaca ggctcttaca ggtgaatcat gtccgaacca gagactttga
     3361 ctgctgcctt gttgtgcccc tcatagcaga atccgggaat aagctggacc tggttattag
     3421 tagaaaccca ctggcttcac agaagtctat agaccaacag agtctaccag gagattggag
     3481 tgaacagaac agtgcttttt tccagcagcc tagccacggt ggtaatttgg agacacgaga
     3541 acccactaat acattatagc aacgcttttt ataaagcagg acaaaagaca atatctacat
     3601 ggtgctaaaa atcctttaag atttctgtga cctttgaagc acagataatc aatcaacgtg
     3661 gcattaactg caagcacagg ggtcttttaa atctctctca tggctcatgt tcacttccct
     3721 tttcaagttg aagaggtttc ttttttggtg accactatgg tatatggtgg gcaatgccct
     3781 gccagtccca acggtagaga aaaataggcc gtcccccaca actctacaat taacatcaga
     3841 ggaaattttt tacaagttca tcttactatc actttttaaa aagagaaaca tctgtttgaa
     3901 aatattctct gtgatgattt ccttaattca ctttgaaatc agtttcttac tatgaagtca
     3961 ttaatgtaag aacttggcca acaaagcttt tcttctcata ggctggctct actaggggaa
     4021 ctagtgtttg gtaaactgct gggactacca caatgggagg ggtacaggta taaaattaag
     4081 ttatcttaaa atgtttcagc aatgatgcac gtaggagacc ataataggtg gtggtaaatg
     4141 ttttgcccac gtataggaat gattttaact aagatgtatg ctattcccta tgcaacaaat
     4201 tatcaaacag gatatgtctt gtgacctgtt ttttttttaa ggacacattt ttaatagctg
     4261 aaaatctctg ataatgaatt agagtgtgta gtaaacatga gaattagtta tattatctta
     4321 tttttaaaat tcaagactaa gaacttcaga gaatgaagag tctattaaaa tgaggttcat
     4381 cttaatgata ggcaaaccaa actcatactg cttgacatgt tttgaaaact ggtaatattg
     4441 agggtgtaca gcacatgtac ttaaaaatga cactggacta tcttttgctc tgagccatgc
     4501 cacttaccga aattgtaaat acatttttca caaatgcatt gccaattatt accatccctc
     4561 aaagcaataa attgtgacag ttgctttaaa tgtttgtcag caactgtttt catttgttca
     4621 gatattttga atagctacac taataactgt tattatttgg tgaacataaa aaaataaata
     4681 gatctgtata ttgatggtag actctccata ttgaaatgat tattttccaa acattttcat
     4741 tttggtcaat aattcaaact accacttagg caaagtattc gaacactgtg tcctttgttt
     4801 aaggaaatat aaaaaaaaaa tcacctttct ttttgtgcaa aaaaaatatt atttcaatca
     4861 catttcagaa ccgccagggc aagaaagtat aaagcaggat catgttaaga aaaaagaaaa
     4921 aaagatcatg agtcacttaa atatgtattt ttatttgtaa caaacaagta ttaaactgta
     4981 aagtattttt gtacaaattt aatactttaa tagcatggta tttatcgtct atgtatggtt
     5041 ttggggaatt caaaattgtt caaatatttg tatggaaaaa aataaaaccc tctaccaaat
     5101 ggaataaaca gtgattttaa aaagccaaat aaagaggata tgcatta
//
