LOCUS       NM_001134407           14706 bp    mRNA    linear   PRI 11-SEP-2024
DEFINITION  Homo sapiens glutamate ionotropic receptor NMDA type subunit 2A
            (GRIN2A), transcript variant 1, mRNA.
ACCESSION   NM_001134407
VERSION     NM_001134407.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 14706)
  AUTHORS   Korinek,M., Candelas Serra,M., Abdel Rahman,F., Dobrovolski,M.,
            Kuchtiak,V., Abramova,V., Fili,K., Tomovic,E., Hrcka Krausova,B.,
            Krusek,J., Cerny,J., Vyklicky,L., Balik,A. and Smejkalova,T.
  TITLE     Disease-Associated Variants in GRIN1, GRIN2A and GRIN2B genes:
            Insights into NMDA Receptor Structure, Function, and
            Pathophysiology
  JOURNAL   Physiol Res 73 (Suppl 1), S413-S434 (2024)
   PUBMED   38836461
  REMARK    GeneRIF: Disease-Associated Variants in GRIN1, GRIN2A and GRIN2B
            genes: Insights into NMDA Receptor Structure, Function, and
            Pathophysiology.
            Review article
REFERENCE   2  (bases 1 to 14706)
  AUTHORS   Raich,I., Lillo,J., Rebassa,J.B., Capo,T., Cordomi,A.,
            Reyes-Resina,I., Pallas,M. and Navarro,G.
  TITLE     Dual Role of NMDAR Containing NR2A and NR2B Subunits in Alzheimer's
            Disease
  JOURNAL   Int J Mol Sci 25 (9), 4757 (2024)
   PUBMED   38731978
  REMARK    GeneRIF: Dual Role of NMDAR Containing NR2A and NR2B Subunits in
            Alzheimer's Disease.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 14706)
  AUTHORS   Xu,Y., Song,R., Perszyk,R.E., Chen,W., Kim,S., Park,K.L.,
            Allen,J.P., Nocilla,K.A., Zhang,J., XiangWei,W., Tankovic,A.,
            McDaniels,E.D., Sheikh,R., Mizu,R.K., Karamchandani,M.M., Hu,C.,
            Kusumoto,H., Pecha,J., Cappuccio,G., Gaitanis,J., Sullivan,J.,
            Shashi,V., Petrovski,S., Jauss,R.T., Lee,H.K., Bozarth,X.,
            Lynch,D.R., Helbig,I., Pierson,T.M., Boerkoel,C.F., Myers,S.J.,
            Lemke,J.R., Benke,T.A., Yuan,H. and Traynelis,S.F.
  TITLE     De novo GRIN variants in M3 helix associated with neurological
            disorders control channel gating of NMDA receptor
  JOURNAL   Cell Mol Life Sci 81 (1), 153 (2024)
   PUBMED   38538865
  REMARK    GeneRIF: De novo GRIN variants in M3 helix associated with
            neurological disorders control channel gating of NMDA receptor.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 14706)
  AUTHORS   Lu,W., Yang,X., Zhong,W., Chen,G., Guo,X., Ye,Q., Xu,Y., Qi,Z.,
            Ye,Y., Zhang,J., Wang,Y., Wang,X., Wang,S., Zhao,Q., Zeng,W.,
            Huang,J., Ma,H. and Xie,J.
  TITLE     METTL14-mediated m6A epitranscriptomic modification contributes to
            chemotherapy-induced neuropathic pain by stabilizing GluN2A
            expression via IGF2BP2
  JOURNAL   J Clin Invest 134 (6), e174847 (2024)
   PUBMED   38319733
  REMARK    GeneRIF: METTL14-mediated m6A epitranscriptomic modification
            contributes to chemotherapy-induced neuropathic pain by stabilizing
            GluN2A expression via IGF2BP2.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 14706)
  AUTHORS   Shepard,N., Baez-Nieto,D., Iqbal,S., Kurganov,E., Budnik,N.,
            Campbell,A.J., Pan,J.Q., Sheng,M. and Farsi,Z.
  TITLE     Differential functional consequences of GRIN2A mutations associated
            with schizophrenia and neurodevelopmental disorders
  JOURNAL   Sci Rep 14 (1), 2798 (2024)
   PUBMED   38307912
  REMARK    GeneRIF: Differential functional consequences of GRIN2A mutations
            associated with schizophrenia and neurodevelopmental disorders.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 14706)
  AUTHORS   Kornau,H.C., Schenker,L.T., Kennedy,M.B. and Seeburg,P.H.
  TITLE     Domain interaction between NMDA receptor subunits and the
            postsynaptic density protein PSD-95
  JOURNAL   Science 269 (5231), 1737-1740 (1995)
   PUBMED   7569905
REFERENCE   7  (bases 1 to 14706)
  AUTHORS   Magnuson,D.S., Knudsen,B.E., Geiger,J.D., Brownstone,R.M. and
            Nath,A.
  TITLE     Human immunodeficiency virus type 1 tat activates
            non-N-methyl-D-aspartate excitatory amino acid receptors and causes
            neurotoxicity
  JOURNAL   Ann Neurol 37 (3), 373-380 (1995)
   PUBMED   7695237
REFERENCE   8  (bases 1 to 14706)
  AUTHORS   Sokolov,B.P. and Prockop,D.J.
  TITLE     A rapid and simple PCR-based method for isolation of cDNAs from
            differentially expressed genes
  JOURNAL   Nucleic Acids Res 22 (19), 4009-4015 (1994)
   PUBMED   7524031
REFERENCE   9  (bases 1 to 14706)
  AUTHORS   Strehlow,V., Myers,K.A., Morgan,A.T., Scheffer,I.E. and Lemke,J.R.
  TITLE     GRIN2A-Related Disorders
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE, Bean LJH,
            Gripp KW and Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   27683935
REFERENCE   10 (bases 1 to 14706)
  AUTHORS   Monyer,H., Sprengel,R., Schoepfer,R., Herb,A., Higuchi,M.,
            Lomeli,H., Burnashev,N., Sakmann,B. and Seeburg,P.H.
  TITLE     Heteromeric NMDA receptors: molecular and functional distinction of
            subtypes
  JOURNAL   Science 256 (5060), 1217-1221 (1992)
   PUBMED   1350383
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC022168.6, AB209695.1,
            U09002.1, AC006531.1 and AC007218.6.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_001134407.2.
            
            Summary: This gene encodes a member of the glutamate-gated ion
            channel protein family. The encoded protein is an
            N-methyl-D-aspartate (NMDA) receptor subunit. NMDA receptors are
            both ligand-gated and voltage-dependent, and are involved in
            long-term potentiation, an activity-dependent increase in the
            efficiency of synaptic transmission thought to underlie certain
            kinds of memory and learning. These receptors are permeable to
            calcium ions, and activation results in a calcium influx into
            post-synaptic cells, which results in the activation of several
            signaling cascades. Disruption of this gene is associated with
            focal epilepsy and speech disorder with or without cognitive
            disability. Alternative splicing results in multiple transcript
            variants. [provided by RefSeq, May 2014].
            
            Transcript Variant: This variant (1) represents the longest
            transcript. Transcript variants 1 and 2 encode the same isoform
            (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: U09002.1, AB209695.1 [ECO:0000332]
            RNAseq introns              :: mixed/partial sample support
                                           SAMEA1966682, SAMEA1968189
                                           [ECO:0000350]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000330684.4/ ENSP00000332549.3
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-22                AC022168.6         24749-24770         c
            23-415              AB209695.1         1-393
            416-1844            U09002.1           1-1429
            1845-1845           AC006531.1         95602-95602         c
            1846-3623           U09002.1           1431-3208
            3624-14706          AC007218.6         120235-131317       c
FEATURES             Location/Qualifiers
     source          1..14706
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="16"
                     /map="16p13.2"
     gene            1..14706
                     /gene="GRIN2A"
                     /gene_synonym="EPND; FESD; GluN2A; LKS; NMDAR2A; NR2A"
                     /note="glutamate ionotropic receptor NMDA type subunit 2A"
                     /db_xref="GeneID:2903"
                     /db_xref="HGNC:HGNC:4585"
                     /db_xref="MIM:138253"
     exon            1..552
                     /gene="GRIN2A"
                     /gene_synonym="EPND; FESD; GluN2A; LKS; NMDAR2A; NR2A"
                     /inference="alignment:Splign:2.1.0"
     exon            553..984
                     /gene="GRIN2A"
                     /gene_synonym="EPND; FESD; GluN2A; LKS; NMDAR2A; NR2A"
                     /inference="alignment:Splign:2.1.0"
     CDS             571..4965
                     /gene="GRIN2A"
                     /gene_synonym="EPND; FESD; GluN2A; LKS; NMDAR2A; NR2A"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; N-methyl-D-aspartate receptor channel, subunit
                     epsilon-1; N-methyl-D-aspartate receptor subunit 2A;
                     N-methyl D-aspartate receptor subtype 2A; glutamate
                     receptor, ionotropic, N-methyl D-aspartate 2A; glutamate
                     receptor ionotropic, NMDA 2A; glutamate ionotropic
                     receptor NMDA 2A"
                     /codon_start=1
                     /product="glutamate receptor ionotropic, NMDA 2A isoform 1
                     precursor"
                     /protein_id="NP_001127879.1"
                     /db_xref="CCDS:CCDS10539.1"
                     /db_xref="GeneID:2903"
                     /db_xref="HGNC:HGNC:4585"
                     /db_xref="MIM:138253"
                     /translation="MGRVGYWTLLVLPALLVWRGPAPSAAAEKGPPALNIAVMLGHSH
                     DVTERELRTLWGPEQAAGLPLDVNVVALLMNRTDPKSLITHVCDLMSGARIHGLVFGD
                     DTDQEAVAQMLDFISSHTFVPILGIHGGASMIMADKDPTSTFFQFGASIQQQATVMLK
                     IMQDYDWHVFSLVTTIFPGYREFISFVKTTVDNSFVGWDMQNVITLDTSFEDAKTQVQ
                     LKKIHSSVILLYCSKDEAVLILSEARSLGLTGYDFFWIVPSLVSGNTELIPKEFPSGL
                     ISVSYDDWDYSLEARVRDGIGILTTAASSMLEKFSYIPEAKASCYGQMERPEVPMHTL
                     HPFMVNVTWDGKDLSFTEEGYQVHPRLVVIVLNKDREWEKVGKWENHTLSLRHAVWPR
                     YKSFSDCEPDDNHLSIVTLEEAPFVIVEDIDPLTETCVRNTVPCRKFVKINNSTNEGM
                     NVKKCCKGFCIDILKKLSRTVKFTYDLYLVTNGKHGKKVNNVWNGMIGEVVYQRAVMA
                     VGSLTINEERSEVVDFSVPFVETGISVMVSRSNGTVSPSAFLEPFSASVWVMMFVMLL
                     IVSAIAVFVFEYFSPVGYNRNLAKGKAPHGPSFTIGKAIWLLWGLVFNNSVPVQNPKG
                     TTSKIMVSVWAFFAVIFLASYTANLAAFMIQEEFVDQVTGLSDKKFQRPHDYSPPFRF
                     GTVPNGSTERNIRNNYPYMHQYMTKFNQKGVEDALVSLKTGKLDAFIYDAAVLNYKAG
                     RDEGCKLVTIGSGYIFATTGYGIALQKGSPWKRQIDLALLQFVGDGEMEELETLWLTG
                     ICHNEKNEVMSSQLDIDNMAGVFYMLAAAMALSLITFIWEHLFYWKLRFCFTGVCSDR
                     PGLLFSISRGIYSCIHGVHIEEKKKSPDFNLTGSQSNMLKLLRSAKNISSMSNMNSSR
                     MDSPKRAADFIQRGSLIMDMVSDKGNLMYSDNRSFQGKESIFGDNMNELQTFVANRQK
                     DNLNNYVFQGQHPLTLNESNPNTVEVAVSTESKANSRPRQLWKKSVDSIRQDSLSQNP
                     VSQRDEATAENRTHSLKSPRYLPEEMAHSDISETSNRATCHREPDNSKNHKTKDNFKR
                     SVASKYPKDCSEVERTYLKTKSSSPRDKIYTIDGEKEPGFHLDPPQFVENVTLPENVD
                     FPDPYQDPSENFRKGDSTLPMNRNPLHNEEGLSNNDQYKLYSKHFTLKDKGSPHSETS
                     ERYRQNSTHCRSCLSNMPTYSGHFTMRSPFKCDACLRMGNLYDIDEDQMLQETGNPAT
                     GEQVYQQDWAQNNALQLQKNKLRISRQHSYDNIVDKPRELDLSRPSRSISLKDRERLL
                     EGNFYGSLFSVPSSKLSGKKSSLFPQGLEDSKRSKSLLPDHTSDNPFLHSHRDDQRLV
                     IGRCPSDPYKHSLPSQAVNDSYLRSSLRSTASYCSRDSRGHNDVYISEHVMPYAANKN
                     NMYSTPRVLNSCSNRRVYKKMPSIESDV"
     exon            985..1577
                     /gene="GRIN2A"
                     /gene_synonym="EPND; FESD; GluN2A; LKS; NMDAR2A; NR2A"
                     /inference="alignment:Splign:2.1.0"
     exon            1578..1692
                     /gene="GRIN2A"
                     /gene_synonym="EPND; FESD; GluN2A; LKS; NMDAR2A; NR2A"
                     /inference="alignment:Splign:2.1.0"
     exon            1693..1898
                     /gene="GRIN2A"
                     /gene_synonym="EPND; FESD; GluN2A; LKS; NMDAR2A; NR2A"
                     /inference="alignment:Splign:2.1.0"
     exon            1899..2067
                     /gene="GRIN2A"
                     /gene_synonym="EPND; FESD; GluN2A; LKS; NMDAR2A; NR2A"
                     /inference="alignment:Splign:2.1.0"
     exon            2068..2221
                     /gene="GRIN2A"
                     /gene_synonym="EPND; FESD; GluN2A; LKS; NMDAR2A; NR2A"
                     /inference="alignment:Splign:2.1.0"
     exon            2222..2347
                     /gene="GRIN2A"
                     /gene_synonym="EPND; FESD; GluN2A; LKS; NMDAR2A; NR2A"
                     /inference="alignment:Splign:2.1.0"
     exon            2348..2577
                     /gene="GRIN2A"
                     /gene_synonym="EPND; FESD; GluN2A; LKS; NMDAR2A; NR2A"
                     /inference="alignment:Splign:2.1.0"
     exon            2578..2738
                     /gene="GRIN2A"
                     /gene_synonym="EPND; FESD; GluN2A; LKS; NMDAR2A; NR2A"
                     /inference="alignment:Splign:2.1.0"
     exon            2739..2926
                     /gene="GRIN2A"
                     /gene_synonym="EPND; FESD; GluN2A; LKS; NMDAR2A; NR2A"
                     /inference="alignment:Splign:2.1.0"
     exon            2927..3165
                     /gene="GRIN2A"
                     /gene_synonym="EPND; FESD; GluN2A; LKS; NMDAR2A; NR2A"
                     /inference="alignment:Splign:2.1.0"
     exon            3166..14706
                     /gene="GRIN2A"
                     /gene_synonym="EPND; FESD; GluN2A; LKS; NMDAR2A; NR2A"
                     /inference="alignment:Splign:2.1.0"
     regulatory      9572..9577
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GRIN2A"
                     /gene_synonym="EPND; FESD; GluN2A; LKS; NMDAR2A; NR2A"
                     /note="hexamer: ATTAAA"
     polyA_site      9606
                     /gene="GRIN2A"
                     /gene_synonym="EPND; FESD; GluN2A; LKS; NMDAR2A; NR2A"
     regulatory      14671..14676
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GRIN2A"
                     /gene_synonym="EPND; FESD; GluN2A; LKS; NMDAR2A; NR2A"
                     /note="hexamer: AATAAA"
     polyA_site      14706
                     /gene="GRIN2A"
                     /gene_synonym="EPND; FESD; GluN2A; LKS; NMDAR2A; NR2A"
                     /note="major polyA site"
ORIGIN      
        1 ctctttctct ccctacctcc ctcgctcagc agctcccggt cgcacaactc ccagcagccg
       61 gcgctgggga ggtggtaggg ggtgcgggtg gaggtgccca gttagcttct cgtgggtggc
      121 caacctgggg ctcccattct cctgtgcacc ctggagtgga gctcccgcta tcagccacag
      181 gggctgagga tccgcactgg gggcgtcgtg ggcatcgggc ttcagcttcc cccctcttgt
      241 ctagcccccc tgggcgcata ggggggcttg tgagcactgc agactgcagc cttcccctca
      301 accctccctc ccctcgttgg tgggctgacc ctgcctctcc ttctctcctc cgccccgtta
      361 gagccctgct gcagagcctc cggctgggat agccgccccc cgtgggggcg atgcggacag
      421 cgcgggacag ccaggggagc gcgctggggc cgcagcatgc gggaacccgc taaacccggt
      481 ggctgctgag gcggccgaga tgctcgtgcg cgcagcgcgc cccactgcat cctcgacctt
      541 ctcgggctac agggaccgtc agtggcgact atgggcagag tgggctattg gaccctgctg
      601 gtgctgccgg cccttctggt ctggcgcggt ccggcgccga gcgcggcggc ggagaagggt
      661 ccccccgcgc taaatattgc ggtgatgctg ggtcacagcc acgacgtgac agagcgcgaa
      721 cttcgaacac tgtggggccc cgagcaggcg gcggggctgc ccctggacgt gaacgtggta
      781 gctctgctga tgaaccgcac cgaccccaag agcctcatca cgcacgtgtg cgacctcatg
      841 tccggggcac gcatccacgg cctcgtgttt ggggacgaca cggaccagga ggccgtagcc
      901 cagatgctgg attttatctc ctcccacacc ttcgtcccca tcttgggcat tcatgggggc
      961 gcatctatga tcatggctga caaggatccg acgtctacct tcttccagtt tggagcgtcc
     1021 atccagcagc aagccacggt catgctgaag atcatgcagg attatgactg gcatgtcttc
     1081 tccctggtga ccactatctt ccctggctac agggaattca tcagcttcgt caagaccaca
     1141 gtggacaaca gctttgtggg ctgggacatg cagaatgtga tcacactgga cacttccttt
     1201 gaggatgcaa agacacaagt ccagctgaag aagatccact cttctgtcat cttgctctac
     1261 tgttccaaag acgaggctgt tctcattctg agtgaggccc gctcccttgg cctcaccggg
     1321 tatgatttct tctggattgt ccccagcttg gtctctggga acacggagct catcccaaaa
     1381 gagtttccat cgggactcat ttctgtctcc tacgatgact gggactacag cctggaggcg
     1441 agagtgaggg acggcattgg catcctaacc accgctgcat cttctatgct ggagaagttc
     1501 tcctacatcc ccgaggccaa ggccagctgc tacgggcaga tggagaggcc agaggtcccg
     1561 atgcacacct tgcacccatt tatggtcaat gttacatggg atggcaaaga cttatccttc
     1621 actgaggaag gctaccaggt gcaccccagg ctggtggtga ttgtgctgaa caaagaccgg
     1681 gaatgggaaa aggtgggcaa gtgggagaac catacgctga gcctgaggca cgccgtgtgg
     1741 cccaggtaca agtccttctc cgactgtgag ccggatgaca accatctcag catcgtcacc
     1801 ctggaggagg ccccattcgt catcgtggaa gacatagacc ccctgaccga gacgtgtgtg
     1861 aggaacaccg tgccatgtcg gaagttcgtc aaaatcaaca attcaaccaa tgaggggatg
     1921 aatgtgaaga aatgctgcaa ggggttctgc attgatattc tgaagaagct ttccagaact
     1981 gtgaagttta cttacgacct ctatctggtg accaatggga agcatggcaa gaaagttaac
     2041 aatgtgtgga atggaatgat cggtgaagtg gtctatcaac gggcagtcat ggcagttggc
     2101 tcgctcacca tcaatgagga acgttctgaa gtggtggact tctctgtgcc ctttgtggaa
     2161 acgggaatca gtgtcatggt ttcaagaagt aatggcaccg tctcaccttc tgcttttcta
     2221 gaaccattca gcgcctctgt ctgggtgatg atgtttgtga tgctgctcat tgtttctgcc
     2281 atagctgttt ttgtctttga atacttcagc cctgttggat acaacagaaa cttagccaaa
     2341 gggaaagcac cccatgggcc ttcttttaca attggaaaag ctatatggct tctttggggc
     2401 ctggtgttca ataactccgt gcctgtccag aatcctaaag ggaccaccag caagatcatg
     2461 gtatctgtat gggccttctt cgctgtcata ttcctggcta gctacacagc caatctggct
     2521 gccttcatga tccaagagga atttgtggac caagtgaccg gcctcagtga caaaaagttt
     2581 cagagacctc atgactattc cccacctttt cgatttggga cagtgcctaa tggaagcacg
     2641 gagagaaaca ttcggaataa ctatccctac atgcatcagt acatgaccaa atttaatcag
     2701 aaaggagtag aggacgcctt ggtcagcctg aaaacgggga agctggacgc tttcatctac
     2761 gatgccgcag tcttgaatta caaggctggg agggatgaag gctgcaagct ggtgaccatc
     2821 gggagtgggt acatctttgc caccaccggt tatggaattg cccttcagaa aggctctcct
     2881 tggaagaggc agatcgacct ggccttgctt cagtttgtgg gtgatggtga gatggaggag
     2941 ctggagaccc tgtggctcac tgggatctgc cacaacgaga agaacgaggt gatgagcagc
     3001 cagctggaca ttgacaacat ggcgggcgta ttctacatgc tggctgccgc catggccctt
     3061 agcctcatca ccttcatctg ggagcacctc ttctactgga agctgcgctt ctgtttcacg
     3121 ggcgtgtgct ccgaccggcc tgggttgctc ttctccatca gcaggggcat ctacagctgc
     3181 attcatggag tgcacattga agaaaagaag aagtctccag acttcaatct gacgggatcc
     3241 cagagcaaca tgttaaaact cctccggtca gccaaaaaca tttccagcat gtccaacatg
     3301 aactcctcaa gaatggactc acccaaaaga gctgctgact tcatccaaag aggttccctc
     3361 atcatggaca tggtttcaga taaggggaat ttgatgtact cagacaacag gtcctttcag
     3421 gggaaagaga gcatttttgg agacaacatg aacgaactcc aaacatttgt ggccaaccgg
     3481 cagaaggata acctcaataa ctatgtattc cagggacaac atcctcttac tctcaatgag
     3541 tccaacccta acacggtgga ggtggccgtg agcacagaat ccaaagcgaa ctctagaccc
     3601 cggcagctgt ggaagaaatc cgtggattcc atacgccagg attcactatc ccagaatcca
     3661 gtctcccaga gggatgaggc aacagcagag aataggaccc actccctaaa gagccctagg
     3721 tatcttccag aagagatggc ccactctgac atttcagaaa cgtcaaatcg ggccacgtgc
     3781 cacagggaac ctgacaacag taagaaccac aaaaccaagg acaactttaa aaggtcagtg
     3841 gcctccaaat accccaagga ctgtagtgag gtcgagcgca cctacctgaa aaccaaatca
     3901 agctccccta gagacaagat ctacactata gatggtgaga aggagcctgg tttccactta
     3961 gatccacccc agtttgttga aaatgtgacc ctgcccgaga acgtggactt cccggacccc
     4021 taccaggatc ccagtgaaaa cttccgcaag ggggactcca cgctgccaat gaaccggaac
     4081 cccttgcata atgaagaggg gctttccaac aacgaccagt ataaactcta ctccaagcac
     4141 ttcaccttga aagacaaggg ttccccgcac agtgagacca gcgagcgata ccggcagaac
     4201 tccacgcact gcagaagctg cctttccaac atgcccacct attcaggcca cttcaccatg
     4261 aggtccccct tcaagtgcga tgcctgcctg cggatgggga acctctatga catcgatgaa
     4321 gaccagatgc ttcaggagac aggtaaccca gccaccgggg agcaggtcta ccagcaggac
     4381 tgggcacaga acaatgccct tcaattacaa aagaacaagc taaggattag ccgtcagcat
     4441 tcctacgata acattgtcga caaacctagg gagctagacc ttagcaggcc ctcccggagc
     4501 ataagcctca aggacaggga acggcttctg gagggaaatt tttacggcag cctgtttagt
     4561 gtcccctcaa gcaaactctc ggggaaaaaa agctcccttt tcccccaagg tctggaggac
     4621 agcaagagga gcaagtctct cttgccagac cacacctccg ataacccttt cctccactcc
     4681 cacagggatg accaacgctt ggttattggg agatgcccct cggaccctta caaacactcg
     4741 ttgccatccc aggcggtgaa tgacagctat cttcggtcgt ccttgaggtc aacggcatcg
     4801 tactgttcca gggacagtcg gggccacaat gatgtgtata tttcggagca tgttatgcct
     4861 tatgctgcaa ataagaataa tatgtactct acccccaggg ttttaaattc ctgcagcaat
     4921 agacgcgtgt acaagaaaat gcctagtatc gaatctgatg tttaaaaatc ttccattaat
     4981 gttttatcta tagggaaata tacgtaatgg ccaatgttct ggagggtaaa tgttggatgt
     5041 ccaatagtgc cctgctaaga ggaagaagat gtagggaggt attttgttgt tgttgttgtt
     5101 ggctcttttg cacacggctt catgccataa tcttccactc aaggaatctt gtgaggtgtg
     5161 tgctgagcat ggcagacacc agataggtga gtccttaacc aaaaataact aactacataa
     5221 gggcaagtct ccgggacatg cctactgggt atgttggcaa taatgatgca ttggatgcca
     5281 atggtgatgt tatgatttcc tatattccaa attccattaa ggtcagccca ccatgtaatt
     5341 ttctcatcag aaatgcctaa tggtttctct aatacagaat aagcaatatg gtgtgcatgt
     5401 aaacctgaca cagacaaaat aaaaacagtt aagaatgcat ctgcactgta gtgcgattga
     5461 catgtgcaag agattaggaa gtttggctcg taacagtttc agctttcttg ttatgccttc
     5521 catcacagcc caggctcacc ccaagaactc caggctcccc taaagaatag caaatcagtg
     5581 tgttcgtgat gactgtgcta ccttcattat agttcatttc caagacacat ctggagccaa
     5641 aggcccgagg gaccctcagg tggggagagc tacaggaatc tctttggatg ttgatgtgtg
     5701 tttctctcac cctcggcttc gatggtcttg ttcagagctg cataaactaa cacatttatg
     5761 tctccgagat ctaagtgtgg atcttctgtc tgtgacacag tggccattgt agtttatccc
     5821 gaagacgcct atgtacgtaa gtttgcattt cctcccttct ggtgatgact cagggttgta
     5881 tagtatctgt taccccttcc ctcccagagt aaccataact cgttccgttt ccaaacagcc
     5941 atggtggtgt ccaattagct gtgtatcgct cttcccagag ttgttaatgt ggtgacatgc
     6001 accaacagcc gtatgtgtac tgtgatctgt aagaagtaca atgccatctg tctgccgaag
     6061 gctagcatgg ttttaggttt atcttccttc acatccagaa attctgttgg acactcactt
     6121 ccaccccaaa ctcctcaaat caaaagcctt caaaacacga ggcactcttg gatctaccct
     6181 gagtatcctc caaactgtgg atacagttta gtgagacaag caatttctcc cttctgagtt
     6241 attctctctg ttggtggcaa accacttcat agcaccaaca gagatgtagg aaaaattcct
     6301 caaagtattt gtcatttctg agtcgcctgc attatcccat tcttattctc ctcaaacctg
     6361 tgcatatatg acatgaaatg atatccattt tttttttaag ttagagacag agaggggaat
     6421 acttatgcat ggggagcctg ttagcacagt gcctgccaca aaaacaagtg cccccgacaa
     6481 gatagttgct atgttatgac actttctcag atcaggattt tctagtttaa aaattaaata
     6541 tcataaaacc agcaaataat attaactaaa ccttttatat agttctactc tgttaattag
     6601 tgtatttcat gtacatgaat atttttgaag tatttgggat aactagccat cagtgtttgc
     6661 agaagacctc agtctgtttt gggccattac ggaaacacta agctaatgta gactcgcctg
     6721 gccatttcct ggtttcctcc tgcattctgc tttacattct tgaagaaagg gataaagtgg
     6781 caggttgaaa aattatccca tttcttctcc tcatggtgtg cgttctgttt tgtaagtatt
     6841 attttcttgc atgtgggcaa gtgtcaggga tgattccctt aaagctcaag gatgcatgga
     6901 atagctccat ctttgggaaa gtacacctga cctacagtgt tcctataatg aaaagtggtt
     6961 gcaataaatt tcagggtcaa tcatagggag acagagggag gcagtgggtg agaggagctt
     7021 cctttcattt ttgaaaggca gaaggggctg actaggtgct ggggatgcat gtgcttgaaa
     7081 atgacatttt atttcccgga tgctgtacct ctgctgcccc aagacccagc catggagaag
     7141 cctggatcga agggcccaga atagtcaccc caacatctga tcctaaatct gaatctccat
     7201 gactgtgtag ccttttagcc ctttctacct tctgcccttc cagtgcattc cagaccttaa
     7261 tccaccccag caaggatcgc ctccatttcc tcatgagtct gggatgtttt gacatgaatg
     7321 aattaaaaag tcttcccaga atacagaaga gtgatttgtc gtcaagccaa agaggctttc
     7381 tgtcttcctc tggtgtcctc taaacccagg gggctgccaa atgccacaga gacctccagg
     7441 gagagtgaga gttatacttg gactgacttc atcggtcaga aagccttccc ctaacagcag
     7501 cattatttct gctattaacc ccatacatct ctggataaag aaactgaaaa cgtggccttt
     7561 ggaagaatca acagtaatgt tgtgaaaaaa atatatattc ttttcactga ccgaagtgtt
     7621 ttttcaaata aataatcaga aatgttatgc tacacaaagt ttatggtatc ccttctctac
     7681 taattcaatg actcttcaat gtgatgatcc caagcaaaaa aaaaaaaaaa aaaaaaaaat
     7741 caactactga tcagatggtc aatttctaag gaaaagatgt agagttattc aaaggtcatc
     7801 taagtcagtt cagtctttgc aaaaagaata tcaggaatgt aacgtccagt ggtaaatgga
     7861 acccacagta tgagaagtaa caagaattaa ataggaagcc tggaagcctg agactataga
     7921 tctatcacag aagattcccg taacatatcc cagctctgag ttcttagatt tgttgatgtc
     7981 aaggagccca aatttctgaa cacaaatgcc tgagtttcag ctggcttaag tcagagctgg
     8041 catccataat gatattttgt taattctaat tggttttccc cacttgagaa ggacccaact
     8101 agtcctcaga taggtacact tgaatgcaaa cggttgggtc tctgtccatg ggtaatagca
     8161 cgcacatgca accaatttaa aatactctgg gtaagagagt cttctagagc ataaactgta
     8221 cttgagaaac ctttgtgctt ctggaaaatg ggtttcatca tccttacatc tgcaggttct
     8281 taggccactt atggtttcaa tgctctgagc tggcaagtcc ctataggtct atctttctgc
     8341 cagagagact tgcaggatta taatctcaca atctcaccac tcattactgt tatgtcctct
     8401 gcaaatctct ctgcgttatt ttagccagca ccatgactgg gcatccacgg attactcttg
     8461 attttctctt ccctgattca caggatgcca gagagtctga gtatttagtt tttctggtga
     8521 aagcattaaa attcacacat tgcagcaaaa tctgtcaaat catttcacgc ttattttctg
     8581 gggagaaaaa tagaaaatta caccttgttt acaaatgaaa tagtttcact taaatgggac
     8641 tctagccaag gcagttaatt agtcaaaaga tccacagctg tcatatagtc acacattgtt
     8701 tattctattt gctatgtcga ccactgatgt gtatttaata ttctgcatcc ttcacaggaa
     8761 atgacaaatg ccctgaatta tttgcaaata gaattgctaa tgaaaaagaa aaaaaatgtt
     8821 gatgttaatt aaatggtgat taaaaattta tctcctttaa atctaaaaag tgcagcttta
     8881 ataatcagtt gtcatataaa taagtacctt gtactgaaat cccttgcggt ttaagaccta
     8941 aataaacact aatttgagaa ctctaaatac cttacagagg agctggatat attcgttaac
     9001 attatgcaca ttcctgtact gtttctttgt gcatagctgt tgtgcatgaa tccttgaaaa
     9061 ggggacatgc gttgtttctg aaatggagcc tgtttgtatt aaccatggct atagtagaca
     9121 tgattggact caaagaaaaa gaagtttgag taggtggaag ctaaatgtgt tgcttgatat
     9181 ttaatgagtg aagggttcta tgtaaaggca attttccttc caaaagtact gtttattttc
     9241 actggagaca aaattagacc tttccagata ccccatctct ttttggtgaa cagggttgaa
     9301 agtgctgtgg ggtctatact tgaaaagttc tcccaatatg cagttactgt ttcccattca
     9361 atccctatac attgtactgt gttcatatct aattgtatca gatgaggtca gcttgccact
     9421 tgatatagat cagagatgat caaatgcact tatcctgtga atacaactgc ttgtgtacac
     9481 atgtatataa tatacatgct tgatttctac aattcacacg taagtgtatt atccaaataa
     9541 aattgtacat attgtaaagt ttatgttaaa aattaaatgg tgatgatggt attaacacat
     9601 ggaaaaccca ttctggttat ttttatggga ggatatttct gtagagaggg cactatattt
     9661 ttttaatctc agttgtaggg aggatgacag agcctaagta gaatatgggt gttggcgttc
     9721 ttggcccctt ctttaaaaaa caaaactctg ggatgagggg tatagaatat attctgctcg
     9781 catttgcttc tgaagacaat gccgatatgg acttgtgtgt gatttcctta aacacatcaa
     9841 agggcataga tttcctattt aaattcctta tcctctttcc tcacctgcac tctttctgtg
     9901 cccagcagta ctttcagtat agtattagct agaagtgcct gcccagaagg gtcttagaaa
     9961 aatagagttc gtagaaccac accaaaagtt aggagaaggg gaggagagat gagaaaaatg
    10021 aagtttacaa atgggcagtt gcctgtttaa gatcatcaag ttagagagta tcagagcgag
    10081 gattagatcc aagacatggt tcaatttctg gctcgattct ctgaccgggc tgacttattt
    10141 ctaggtttct ttcaagggga aactcctgag tagctctgga ataagagcca gagatcccca
    10201 taaggctgcc ctggtttttg ttttgttttg ttttttgttt ttgccacttg ccccagaaag
    10261 acctacccat gcttctttga atttggtttt cctttaaact ccctcacaat ccctgtctta
    10321 aagttcctag aagagaaaac ttttctcaga gaccgtctcc ttaggaaaaa tcaacaggga
    10381 tattgtttaa aacctcaaac tgaagataaa aggctggcag tgaccattga aataattgag
    10441 gtctatgtat catgcattga ggaagaacca agcacattga ccagctaata ggacttcact
    10501 gaatgtccac tttattttga aagccagttt gggattgtgg gggaggggat gcaaaataaa
    10561 tagaaaatat agtctcttcc cataagtagc ttatggttta gaagttcagt tctctaatac
    10621 aaaggataac caaggaccca tgcaatgcaa acagagacta aacagcaaaa attagaaatg
    10681 acaactaaag ttgtagccag gaaaaaaaaa ataatctgat tctatgagca tacctttttt
    10741 aaaaaaaaaa aaaaagtcct tccctaaacc tttaagtaac tccggagaat agagttagtg
    10801 gtcccggaaa tgcagttgat ttattttaat cttcaaataa tggacttagg agacccagga
    10861 gctgctaacc aggtgaacac actcacactc ctacattttt gcacaggaag gcttttgtct
    10921 ggttccaacc agttctccag tccttgccaa atcatgcctc ctcccagcct tgcattatga
    10981 catgtgcctc aagaaaaggt tgaaaaaatt ccccaaagga ttagaaaata gaccatgatg
    11041 taaaaagacg aagcatttcc ctaaagcttt tttgtttggt tttgtttttg acatttgaag
    11101 aacccttctg atacaccagg gtatgaatgt atcagggaag catagaggga aaattcttta
    11161 tcaagttcca agtgcctact gctgtggctg acagaaagca tcaggttccc tagagagaaa
    11221 acggggcaaa gagatggcaa aaggaataga ggtgtatttc aggaaggatg gcgaggtgga
    11281 gagggagctt ttgtcactct gtctatccca ttctgtgcat tcttggaatg ttacattttc
    11341 tgcttatcgc aagtacttgc catggatctg cacctgaatt tgaaaatatc aaaatatatc
    11401 taaattgagt gtaggcgata ttctaggcat atcctagaat gtgatcctca aaacaacctt
    11461 aagatataaa tatgattgct cccattttac agataaggaa actgaggctc aggtttttct
    11521 caacctcgtc agcaactaga attagctagt aaatccctaa cgttagacat attttcaaag
    11581 tgcctggtct cctccaggga ggtcagaata gaagtcagaa agagaggtgt gctcttttga
    11641 ggttcctggg catataccct tgagtatcag cctctgtgac agggaagtct gttagggtct
    11701 tacccctcat tgcctgatct gtgtgtatgg agaacttctg gggatctcac ctctgttcat
    11761 agttccttct gagacttgaa cactgtttgt gtctcccttt catatgcttg cacttagcat
    11821 ttaaaaaaat ttctggggag ttattttctc tacttagcat gtgcctaatc ttaaatatct
    11881 ctttcctaat gtctcatctt tttatcagtc aacatcaggg ctaattccat taaacatata
    11941 tacatagtac atgctctcat gtgtgtgggt atgcacacac acacacacag ccatgtgacc
    12001 ctccattaaa gtcctcctaa atattgtgac ccatgttcaa agaggtgata ttatttgcaa
    12061 gttgactatt cctcctattt catgtctatg tttggttatc tttgccaggc agatggaatg
    12121 agtttcagag gtgggacagg gaataggaaa tagagtttct gcatgaatga tggagagctt
    12181 tggtagtcag aatgattgag gaccagctat taaaatgttg ttgttgtttt ccttcttggt
    12241 ctgttccttt ttttttttct tttccaaatt gtcttagaga taattcccgg ctcacttagc
    12301 attaggataa tgacggttat tctgtcccac tgaagagtgt tgaactggaa cgcttccacc
    12361 gggctttata ggtacccaga atggcctaat gtttaacata tcctgcaggc atgcccctga
    12421 gcacacactg cctttcctgc attcccccct ctccatggtc taagccacat ttctttcctg
    12481 gcatttatat ttactgagag gtgaggggtg cattaggccc atttgatgga tgaggacact
    12541 agcggggatt tgaaggaatg tctttctggg agtctccatg aattctctgg agagttagct
    12601 aacttaatgc aagttaagtg ctagtctatg agaaaaattt ctcagctgtt ttcatctttc
    12661 aagctggcag taaaaagcct tttaagacag aaagattatt atttgttatt tggagatcct
    12721 gttgttgggt ctacaagtgc gtgaccatgt tctccttgga atgttgagct atgcagaaat
    12781 ggagcactcc aaagaacgat ttgaccaata gcatttcttc tctgggggtt gtatttcaaa
    12841 gcatgcaact ctccagggaa ccagaactaa attgcttaaa atgaagtcat tcctcagatt
    12901 aacttcctca gataaagtgt cagcggtctg cagaaacgaa gaagacaaaa ctgagattat
    12961 cactcataat tctcttactt actatgtcag tgaaacaatg agtttgcatt tttgcaatcc
    13021 tagaacattc ttcattagcc ctgggtcatg acctcttcca gttaattctc tttcacacct
    13081 ttaggaaaga tttaagatga accttcaata ggatattaac ataactcata gccaatacca
    13141 cagctgcctt tcaaattaat gaggttaatt gttctccagc aaacatgagt ttgtctttgg
    13201 cattttaaat gcttcccatt gatctgacat tttgctgttt caagttttaa agggctcaaa
    13261 tcaaagacta ttgataactg agcaaagagc gaagatccag aaatacgaaa acattgtctt
    13321 tttttttcca tgaaaaacaa tcatagcctt ttgaattcaa tcgaagtttc tacattagcc
    13381 atctaagact tatttaatta tttctgttct cagtcaagct aattcaagtg aatgaacagt
    13441 attgactttt aaaatctttt ttaaattttt ttaaatcttt agtttattaa gtttgtagaa
    13501 aagctctggg gccatgacca cttacgtaaa tgtttcagtt taaaaacaaa agattcaggc
    13561 ctctaatttg agccaaatcc aggtgatctt gtttgaaatt tttgatgaat ttgaaaagat
    13621 gaaagtggaa cttttaacat tcatgttccc caaatttttc actgggaagg gatgctaatt
    13681 gcctacttaa gatataagtt caagaataac attttcatag aaaattcaga aaactgcttg
    13741 acacagcagt gacatagtta gatgtggctc agatgccttc caaacctgag ggtccccaaa
    13801 gatttcttta ccagttgttt ttaactatga atcttaatct tgttcattcc cctgccaaaa
    13861 caaatttaaa agcataaacc tgctgaatta attggcagaa ttcaccatag tttatatcac
    13921 cccagaatgt ttatgtgtat atgtttatgt atagtatata tatttcaagc tgaatgttca
    13981 gaaagaataa ggtgagtttc ttggtagctt gaataagaat tagatcataa aaattataaa
    14041 taatggcctt ctcaaagagg ataaatgatt catttttcac tcacgtatta ataatatgac
    14101 ttgttgcata atacacaagc tggtcattgc ctgcaagaaa gttattgaat taagcagcta
    14161 tccaggttta tgtctgagtc catttttaac ccaacaaggc taggattgaa ctccatatgc
    14221 agagggtttc tattcacagc tacattcaaa cagaatccaa aacccacatc agaactgact
    14281 tgatgtcttg tatggtggag ctgaaagcaa gctgtctgct ctaagcaatg gtcttcattt
    14341 gggaattaat gttcactgat caactgcagc agtttatatc tgtcatgttt taatccactg
    14401 catgaggaag tgctaataag tgtattaaat atttccaagc atagctacaa aattatatgt
    14461 ttgcttaaca cctttctatg atagaggaag tatatactat ggttgtcttc ttgtagtatg
    14521 tcttttaatt ttgttcaatg tgttatcaac tgttttaagg aatggggatt gaaattcgtg
    14581 tgcaaactga tatgtatata actcctgagg caacagaagt gcccggtcat atggtgtaaa
    14641 aatgtaaata caggaaaatt tcatttttct aataaagata atttctgcca attgatatga
    14701 aaatga
//
