LOCUS       NM_014619               5815 bp    mRNA    linear   PRI 07-APR-2024
DEFINITION  Homo sapiens glutamate ionotropic receptor kainate type subunit 4
            (GRIK4), transcript variant 2, mRNA.
ACCESSION   NM_014619 XM_005271519
VERSION     NM_014619.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5815)
  AUTHORS   Herbrechter,R., Hube,N., Buchholz,R. and Reiner,A.
  TITLE     Splicing and editing of ionotropic glutamate receptors: a
            comprehensive analysis based on human RNA-Seq data
  JOURNAL   Cell Mol Life Sci 78 (14), 5605-5630 (2021)
   PUBMED   34100982
REFERENCE   2  (bases 1 to 5815)
  AUTHORS   Pu,M., Wang,X. and Zhang,J.
  TITLE     Impact of GRIK4 gene polymorphisms on cognitive dysfunction in
            patients with major depression
  JOURNAL   Rev Neurol (Paris) 176 (10), 823-828 (2020)
   PUBMED   32245654
  REMARK    GeneRIF: Impact of GRIK4 gene polymorphisms on cognitive
            dysfunction in patients with major depression.
REFERENCE   3  (bases 1 to 5815)
  AUTHORS   Poweleit,E.A., Aldrich,S.L., Martin,L.J., Hahn,D., Strawn,J.R. and
            Ramsey,L.B.
  TITLE     Pharmacogenetics of Sertraline Tolerability and Response in
            Pediatric Anxiety and Depressive Disorders
  JOURNAL   J Child Adolesc Psychopharmacol 29 (5), 348-361 (2019)
   PUBMED   31066578
  REMARK    GeneRIF: In addition, while GRIK4 was not associated with
            sertraline tolerability and response, both HTR2A and SLC6A4 could
            influence tolerability and accelerate treatment response in
            pediatric patients.
REFERENCE   4  (bases 1 to 5815)
  AUTHORS   Koromina,M., Flitton,M., Mellor,I.R. and Knight,H.M.
  TITLE     A kainate receptor GluK4 deletion, protective against bipolar
            disorder, is associated with enhanced cognitive performance across
            diagnoses in the TwinsUK cohort
  JOURNAL   World J Biol Psychiatry 20 (5), 393-401 (2019)
   PUBMED   29243543
  REMARK    GeneRIF: GluK4 deletion carriers who had a mental health problem
            (predominately depression) showed better performance in
            visuo-spatial ability and mental processing speed compared to
            individuals with mental health problems homozygous for the
            insertion.
REFERENCE   5  (bases 1 to 5815)
  AUTHORS   Ren,D., Xu,F., Bi,Y., Niu,W., Zhang,R., Hu,J., Guo,Z., Cao,Y.,
            Huang,X., Wu,X., Yang,F., Wang,L., Li,W., Xu,Y., He,L., Yu,T.,
            Li,X. and He,G.
  TITLE     No association of GRIK4 polymorphisms with schizophrenia in the
            Chinese Han population
  JOURNAL   Psychiatr Genet 27 (4), 159-160 (2017)
   PUBMED   28658089
  REMARK    GeneRIF: the GRIK4 polymorphisms genotypes were distributed as
            follows: rs79526501: CC696: 16, CG302: 274, GG31: 35; rs11218016:
            CC455: 483, CT473: 436, TT101: 108; and rs6589847: AA32: 29, AG307:
            314, GG690: 686. However, there was no significant difference in
            allelic or genotypic frequency distributions between patients and
            controls. We did not find any significant association between GRIK4
            polymorphisms and schizophrenia.
REFERENCE   6  (bases 1 to 5815)
  AUTHORS   Darstein,M., Petralia,R.S., Swanson,G.T., Wenthold,R.J. and
            Heinemann,S.F.
  TITLE     Distribution of kainate receptor subunits at hippocampal mossy
            fiber synapses
  JOURNAL   J Neurosci 23 (22), 8013-8019 (2003)
   PUBMED   12954862
REFERENCE   7  (bases 1 to 5815)
  AUTHORS   Ritter,L.M., Unis,A.S. and Meador-Woodruff,J.H.
  TITLE     Ontogeny of ionotropic glutamate receptor expression in human fetal
            brain
  JOURNAL   Brain Res Dev Brain Res 127 (2), 123-133 (2001)
   PUBMED   11334999
REFERENCE   8  (bases 1 to 5815)
  AUTHORS   Szpirer,C., Molne,M., Antonacci,R., Jenkins,N.A., Finelli,P.,
            Szpirer,J., Riviere,M., Rocchi,M., Gilbert,D.J., Copeland,N.G. et
            al.
  TITLE     The genes encoding the glutamate receptor subunits KA1 and KA2
            (GRIK4 and GRIK5) are located on separate chromosomes in human,
            mouse, and rat
  JOURNAL   Proc Natl Acad Sci U S A 91 (25), 11849-11853 (1994)
   PUBMED   7527545
REFERENCE   9  (bases 1 to 5815)
  AUTHORS   Wenthold,R.J., Trumpy,V.A., Zhu,W.S. and Petralia,R.S.
  TITLE     Biochemical and assembly properties of GluR6 and KA2, two members
            of the kainate receptor family, determined with subunit-specific
            antibodies
  JOURNAL   J Biol Chem 269 (2), 1332-1339 (1994)
   PUBMED   8288598
REFERENCE   10 (bases 1 to 5815)
  AUTHORS   Kamboj,R.K., Schoepp,D.D., Nutt,S., Shekter,L., Korczak,B.,
            True,R.A., Rampersad,V., Zimmerman,D.M. and Wosnick,M.A.
  TITLE     Molecular cloning, expression, and pharmacological characterization
            of humEAA1, a human kainate receptor subunit
  JOURNAL   J Neurochem 62 (1), 1-9 (1994)
   PUBMED   8263508
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA246271.1, AK292726.1,
            AP001929.5, AW016926.1 and DB306924.1.
            
            On Oct 11, 2019 this sequence version replaced NM_014619.4.
            
            Summary: This gene encodes a protein that belongs to the
            glutamate-gated ionic channel family. Glutamate functions as the
            major excitatory neurotransmitter in the central nervous system
            through activation of ligand-gated ion channels and G
            protein-coupled membrane receptors. The protein encoded by this
            gene forms functional heteromeric kainate-preferring ionic channels
            with the subunits encoded by related gene family members.
            Alternatively spliced transcript variants encoding different
            isoforms have been found for this gene. [provided by RefSeq, Sep
            2013].
            
            Transcript Variant: This variant (2) differs in the 5' UTR and
            encodes the same isoform (1), compared to variant 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK292726.1, SRR14372079.1507786.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000527524.8/ ENSP00000435648.2
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-11                DA246271.1         4-14
            12-1572             AK292726.1         1-1561
            1573-1573           AP001929.5         23645-23645
            1574-2118           AK292726.1         1563-2107
            2119-2119           AP001929.5         75253-75253
            2120-3332           AK292726.1         2109-3321
            3333-5336           AP001929.5         104777-106780
            5337-5714           AW016926.1         15-392              c
            5715-5813           DB306924.1         1-99                c
            5814-5815           AP001929.5         107258-107259
FEATURES             Location/Qualifiers
     source          1..5815
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11q23.3"
     gene            1..5815
                     /gene="GRIK4"
                     /gene_synonym="EAA1; GluK4; GluK4-2; GRIK; KA1"
                     /note="glutamate ionotropic receptor kainate type subunit
                     4"
                     /db_xref="GeneID:2900"
                     /db_xref="HGNC:HGNC:4582"
                     /db_xref="MIM:600282"
     exon            1..140
                     /gene="GRIK4"
                     /gene_synonym="EAA1; GluK4; GluK4-2; GRIK; KA1"
                     /inference="alignment:Splign:2.1.0"
     exon            141..248
                     /gene="GRIK4"
                     /gene_synonym="EAA1; GluK4; GluK4-2; GRIK; KA1"
                     /inference="alignment:Splign:2.1.0"
     exon            249..380
                     /gene="GRIK4"
                     /gene_synonym="EAA1; GluK4; GluK4-2; GRIK; KA1"
                     /inference="alignment:Splign:2.1.0"
     CDS             299..3169
                     /gene="GRIK4"
                     /gene_synonym="EAA1; GluK4; GluK4-2; GRIK; KA1"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 2; excitatory amino acid receptor 1; glutamate
                     receptor KA1; glutamate receptor, ionotropic, kainate 4;
                     putative NMDtranscript(altAcc_e11); GluK4(alt_5'UTR)"
                     /codon_start=1
                     /product="glutamate receptor ionotropic, kainate 4 isoform
                     1 precursor"
                     /protein_id="NP_055434.2"
                     /db_xref="CCDS:CCDS8433.1"
                     /db_xref="GeneID:2900"
                     /db_xref="HGNC:HGNC:4582"
                     /db_xref="MIM:600282"
                     /translation="MPRVSAPLVLLPAWLVMVACSPHSLRIAAILDDPMECSRGERLS
                     ITLAKNRINRAPERLGKAKVEVDIFELLRDSEYETAETMCQILPKGVVAVLGPSSSPA
                     SSSIISNICGEKEVPHFKVAPEEFVKFQFQRFTTLNLHPSNTDISVAVAGILNFFNCT
                     TACLICAKAECLLNLEKLLRQFLISKDTLSVRMLDDTRDPTPLLKEIRDDKTATIIIH
                     ANASMSHTILLKAAELGMVSAYYTYIFTNLEFSLQRMDSLVDDRVNILGFSIFNQSHA
                     FFQEFAQSLNQSWQENCDHVPFTGPALSSALLFDAVYAVVTAVQELNRSQEIGVKPLS
                     CGSAQIWQHGTSLMNYLRMVELEGLTGHIEFNSKGQRSNYALKILQFTRNGFRQIGQW
                     HVAEGLSMDSHLYASNISDTLFNTTLVVTTILENPYLMLKGNHQEMEGNDRYEGFCVD
                     MLKELAEILRFNYKIRLVGDGVYGVPEANGTWTGMVGELIARKADLAVAGLTITAERE
                     KVIDFSKPFMTLGISILYRVHMGRKPGYFSFLDPFSPGVWLFMLLAYLAVSCVLFLVA
                     RLTPYEWYSPHPCAQGRCNLLVNQYSLGNSLWFPVGGFMQQGSTIAPRALSTRCVSGV
                     WWAFTLIIISSYTANLAAFLTVQRMDVPIESVDDLADQTAIEYGTIHGGSSMTFFQNS
                     RYQTYQRMWNYMYSKQPSVFVKSTEEGIARVLNSNYAFLLESTMNEYYRQRNCNLTQI
                     GGLLDTKGYGIGMPVGSVFRDEFDLAILQLQENNRLEILKRKWWEGGKCPKEEDHRAK
                     GLGMENIGGIFVVLICGLIVAIFMAMLEFLWTLRHSEATEVSVCQEMVTELRSIILCQ
                     DSIHPRRRRAAVPPPRPPIPEERRPRGTATLSNGKLCGAGEPDQLAQRLAQEAALVAR
                     GCTHIRVCPECRRFQGLRARPSPARSEESLEWEKTTNSSEPE"
     exon            381..545
                     /gene="GRIK4"
                     /gene_synonym="EAA1; GluK4; GluK4-2; GRIK; KA1"
                     /inference="alignment:Splign:2.1.0"
     exon            546..643
                     /gene="GRIK4"
                     /gene_synonym="EAA1; GluK4; GluK4-2; GRIK; KA1"
                     /inference="alignment:Splign:2.1.0"
     exon            644..809
                     /gene="GRIK4"
                     /gene_synonym="EAA1; GluK4; GluK4-2; GRIK; KA1"
                     /inference="alignment:Splign:2.1.0"
     exon            810..988
                     /gene="GRIK4"
                     /gene_synonym="EAA1; GluK4; GluK4-2; GRIK; KA1"
                     /inference="alignment:Splign:2.1.0"
     exon            989..1042
                     /gene="GRIK4"
                     /gene_synonym="EAA1; GluK4; GluK4-2; GRIK; KA1"
                     /inference="alignment:Splign:2.1.0"
     exon            1043..1204
                     /gene="GRIK4"
                     /gene_synonym="EAA1; GluK4; GluK4-2; GRIK; KA1"
                     /inference="alignment:Splign:2.1.0"
     exon            1205..1357
                     /gene="GRIK4"
                     /gene_synonym="EAA1; GluK4; GluK4-2; GRIK; KA1"
                     /inference="alignment:Splign:2.1.0"
     exon            1358..1462
                     /gene="GRIK4"
                     /gene_synonym="EAA1; GluK4; GluK4-2; GRIK; KA1"
                     /inference="alignment:Splign:2.1.0"
     exon            1463..1570
                     /gene="GRIK4"
                     /gene_synonym="EAA1; GluK4; GluK4-2; GRIK; KA1"
                     /inference="alignment:Splign:2.1.0"
     exon            1571..1774
                     /gene="GRIK4"
                     /gene_synonym="EAA1; GluK4; GluK4-2; GRIK; KA1"
                     /inference="alignment:Splign:2.1.0"
     exon            1775..1888
                     /gene="GRIK4"
                     /gene_synonym="EAA1; GluK4; GluK4-2; GRIK; KA1"
                     /inference="alignment:Splign:2.1.0"
     exon            1889..1998
                     /gene="GRIK4"
                     /gene_synonym="EAA1; GluK4; GluK4-2; GRIK; KA1"
                     /inference="alignment:Splign:2.1.0"
     exon            1999..2172
                     /gene="GRIK4"
                     /gene_synonym="EAA1; GluK4; GluK4-2; GRIK; KA1"
                     /inference="alignment:Splign:2.1.0"
     exon            2173..2338
                     /gene="GRIK4"
                     /gene_synonym="EAA1; GluK4; GluK4-2; GRIK; KA1"
                     /inference="alignment:Splign:2.1.0"
     exon            2339..2564
                     /gene="GRIK4"
                     /gene_synonym="EAA1; GluK4; GluK4-2; GRIK; KA1"
                     /inference="alignment:Splign:2.1.0"
     exon            2565..2693
                     /gene="GRIK4"
                     /gene_synonym="EAA1; GluK4; GluK4-2; GRIK; KA1"
                     /inference="alignment:Splign:2.1.0"
     exon            2694..2812
                     /gene="GRIK4"
                     /gene_synonym="EAA1; GluK4; GluK4-2; GRIK; KA1"
                     /inference="alignment:Splign:2.1.0"
     exon            2813..5815
                     /gene="GRIK4"
                     /gene_synonym="EAA1; GluK4; GluK4-2; GRIK; KA1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4907..4912
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GRIK4"
                     /gene_synonym="EAA1; GluK4; GluK4-2; GRIK; KA1"
                     /note="hexamer: AATAAA"
     polyA_site      4930
                     /gene="GRIK4"
                     /gene_synonym="EAA1; GluK4; GluK4-2; GRIK; KA1"
                     /note="major polyA site"
     regulatory      5690..5695
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GRIK4"
                     /gene_synonym="EAA1; GluK4; GluK4-2; GRIK; KA1"
                     /note="hexamer: AATAAA"
     polyA_site      5714
                     /gene="GRIK4"
                     /gene_synonym="EAA1; GluK4; GluK4-2; GRIK; KA1"
                     /note="major polyA site"
     regulatory      5797..5802
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GRIK4"
                     /gene_synonym="EAA1; GluK4; GluK4-2; GRIK; KA1"
                     /note="hexamer: AATAAA"
     polyA_site      5815
                     /gene="GRIK4"
                     /gene_synonym="EAA1; GluK4; GluK4-2; GRIK; KA1"
ORIGIN      
        1 agactgcctt cagctgcggg cggatcgggc tggagccgcc acggctgctg cggaagagga
       61 aaaacggcca acagcagccc cgcggccggc ccggcagcgc ccggcccccg gctcagcccc
      121 cggagtgcgg agccgaccag cagatacttt ctgagtgcct actatgtgcc agcctgtgct
      181 aggcactgag gacacaggtg gaaaagcccg aattgctccc tgctctcctg gcgctcatca
      241 ccccggagag ttatgtcatg cccaggccag cagggggctc catgaggatt catagaagat
      301 gccccgcgtc tcggcgcctt tggtgctgct tcctgcgtgg ctcgtgatgg tcgcctgcag
      361 cccgcactcc ttgaggatcg ctgctatctt ggacgacccc atggagtgca gcagagggga
      421 gcggctctcc atcaccctgg ccaagaaccg catcaaccgc gctcctgaga ggctgggcaa
      481 ggccaaggtc gaagtggaca tctttgagct tctcagagac agcgagtacg agactgcaga
      541 aaccatgtgt cagatcctcc ccaagggggt ggtcgctgtc ctcggaccat cgtccagccc
      601 agcctccagc tccatcatca gcaacatctg tggagagaag gaggtccctc acttcaaagt
      661 ggccccagag gagttcgtca agttccagtt ccagagattc acaaccctga acctccaccc
      721 cagcaacact gacatcagcg tggctgtagc tgggatcctg aacttcttca actgcaccac
      781 cgcctgcctc atctgtgcca aagcagaatg ccttttaaac ctagagaagc tgctccggca
      841 attccttatc tccaaggaca cgctgtccgt ccgcatgctg gatgacaccc gggaccccac
      901 cccgctcctc aaggagatcc gggacgacaa gaccgccacc atcatcatcc acgccaacgc
      961 ctccatgtcc cacaccatcc tcctgaaggc agccgaactt gggatggtgt cagcctatta
     1021 cacatacatc ttcactaatc tggagttctc actccagaga atggacagcc ttgtggatga
     1081 tcgtgtcaac atcctgggat tttccatttt caaccaatcc catgctttct tccaagagtt
     1141 tgcccagagc ctcaaccagt cctggcagga gaactgtgac catgtgccct tcactgggcc
     1201 tgcgctctcc tcggccctgc tgtttgatgc tgtctatgct gtggtgactg cggtgcagga
     1261 actgaaccgg agccaagaga tcggcgtgaa gcccttgtcc tgcggctcgg cccagatctg
     1321 gcagcacggc accagcctca tgaactacct gcgcatggta gaattggaag gtcttaccgg
     1381 ccacattgaa ttcaacagca aaggccagag gtccaactac gctttgaaaa tcttacagtt
     1441 cacaaggaat ggttttcggc agatcggcca gtggcacgtg gcagagggcc tcagcatgga
     1501 cagccacctc tatgcctcca acatctcgga cactctcttc aacaccaccc tggtcgtcac
     1561 caccatcctg gagaacccat atttaatgct gaaggggaac caccaggaga tggaaggcaa
     1621 tgaccgctac gagggcttct gtgtggacat gctcaaggag ctggcagaga tcctccgatt
     1681 caactacaag atccgcctgg ttggggatgg cgtgtacggc gttcccgagg ccaacggcac
     1741 ctggacggga atggtcgggg agctgatcgc taggaaagca gatctggctg tggcaggcct
     1801 caccattaca gctgaacggg agaaggtgat tgatttctct aagccattca tgactctggg
     1861 aattagcatt ctttaccgcg ttcatatggg acgcaaaccc ggctatttct ccttcctgga
     1921 cccattttct ccgggcgtct ggctcttcat gcttctagcc tatctggccg tcagctgtgt
     1981 cctcttcctg gtggctcggt tgacgcccta cgagtggtac agcccacacc catgtgccca
     2041 gggccggtgc aacctcctgg tgaaccagta ctccctgggc aacagcctct ggtttccggt
     2101 cggggggttc atgcagcaag gctccaccat cgcccctcgc gccttatcca cccgctgtgt
     2161 cagtggcgtc tggtgggcat tcacgctgat catcatctca tcctacacgg ccaacctggc
     2221 agccttcctg accgtgcagc gcatggatgt gcccattgag tcagtggatg acctggctga
     2281 ccagaccgcc attgaatatg gcacaattca cggaggctcc agcatgacct tcttccaaaa
     2341 ttcccgctac cagacctacc aacgcatgtg gaattacatg tattccaagc agcccagcgt
     2401 gttcgtgaag agcacagagg agggaatcgc cagggtgttg aattccaact acgccttcct
     2461 cctggaatcc accatgaacg agtactatcg gcagcgaaac tgcaacctca ctcagattgg
     2521 gggcctgctg gacaccaagg gctatgggat tggcatgcca gtcggctcgg ttttccggga
     2581 cgagtttgat ctggccattc tccagctgca ggagaacaac cgcctggaga tcctgaagcg
     2641 caaatggtgg gaaggaggga agtgccccaa ggaggaagat cacagagcta aaggcctggg
     2701 aatggagaat attggtggaa tctttgtggt tcttatttgt ggcttaatcg tggccatttt
     2761 tatggctatg ttggagtttt tatggactct cagacactca gaagcaactg aggtgtccgt
     2821 ctgccaggag atggtgaccg agctgcgcag cattatcctg tgtcaggaca gtatccaccc
     2881 ccgccggcgg cgcgccgcag tcccgccgcc ccggcccccc atccccgagg agcgccgacc
     2941 gcggggcacg gcgacgctca gcaacgggaa gctgtgcggg gcaggggagc ccgaccagct
     3001 cgcgcagaga ctggcgcagg aggccgccct ggtggcccgc ggctgcacgc acatccgcgt
     3061 ctgccccgag tgccgccgct tccagggcct gcgggcacgg ccgtcgcccg cccgcagcga
     3121 ggagagcctg gagtgggaga aaaccaccaa cagcagcgag cccgagtagt cccggaggcc
     3181 acaggacgcg cagaggccgg gcggggcggg aggggagggg cggggcgggc gctgctgtca
     3241 gccgccagcc ggaacttgta cagcgtcgac acctctccag atttcggatc cagtcacttt
     3301 tcaaaaagat caaggagcct gacgccccag ccagagaccg cgcccggtca gggagcaggg
     3361 tccacccgga aacgttgcac ccaaagggca aaggacggcc ctccctcctg ggcacaagga
     3421 cccatcttct cccagtgggt ctttccctct cgccaaaata acaagagtat agggtggggg
     3481 gtccctaccc agaccagtcc aatgaattgg tggaatcatc agttgaattt ccccctagtc
     3541 aggggccaat gtaccctccg tctagttctt acagaaaaaa aaaaaattaa acagggaagt
     3601 ttttcttttc tggatttgta tatttttgtt aatgttcttt tcccttttct ttcctcctct
     3661 ccttttcttc tttgtcatct tctcagtcct gttaatttgt tttgtgtttt ttggaggggg
     3721 aggctgggtt agggaatgga agcctaaata atccctattt cttctttttc ctgaattttg
     3781 gaatattgcg ttaccagtgc atccgatttc aggtgcttaa ctctctgtat ggtgactgag
     3841 gggcctgcag gagcacagga gaaggagtct tgggaagaat cagatggaga gtctcaaaag
     3901 tccattgcag taaactttga gatgccaaaa agcaattaac tcatcctata ctcagacctt
     3961 gaaacgcctg gaatgtcaag ggttaatctg tcttttcttt ccctttctct ttctgactgt
     4021 cactattact gggtttgatt tgatcttttt gtgaatgtag tctctgaaga cagacagggg
     4081 aggagaacag aatgcacaaa gtatcctagg actttgttta aggagcggaa agagcagata
     4141 gaattttccc ataccatggc cttggcttcc ggtggaacgt gccgttcaca gaggcggaag
     4201 gactgggcca ccccgttggt atggtgcata ctttgtcaac tgcatctctt ttcaaccaaa
     4261 ctcaaatgag tggctgccta tgaagccaga cccccaagac tatcatggat tgggtggttt
     4321 cttgctactt agacgtgaga cctgggtgca agtgtatgtg tgtgtatgtg tgcgcgcgtg
     4381 tgcgtgtgtg tatttattca acagaaagac cttaagtatt caagaatttg ctaggttcct
     4441 gggagagagg tggagtctcc tagtcaatac acgggcctgg gaaccaggaa ctcttgagtc
     4501 gtaacctcag cttgagcagt agccctccca ccttgtatga ctttaggtaa agcatttaac
     4561 ttctcttcca tctaccaaat aggagttact gatgctgtcc aggttaccag gtgactgcta
     4621 gtgactgctc tccatgtgag aagtttgcag agtccagagc acacatctga tgggaggggc
     4681 gaggctttct acagctgctt gacctttgct tggcttcact aattgggagt ttcacgcaga
     4741 gttctcattg gctgatggaa aacaaattct atgcaatttc tgattgtaaa cttagaaaat
     4801 tcagagagaa acaaacagcc gctaccaacc acctccacct ccaccaccgc tgccaccacc
     4861 ttgaatggaa ttgtcctcac ttttgttgct gttgcaaatg tactaaaata aagacatctt
     4921 tggaagatga cgagcttaat tcatgttttt gccatttggg ttggtcggca tcctctttat
     4981 gccaggacaa tcagttaggg ctgccgtcct gggggccaaa tggtaccctg attattcctg
     5041 tgagggatac tcgttttgtc ctcacctgac ctcactcatg aatttgaatc ctttatcttt
     5101 tttctcttta accatcaaat gacaacaagc tgtgcaagac acttgtgtct ttttttaaac
     5161 cttgccttga tcctcgtgga gaaacgtgga tttggtggga agggcacccg aaggccagct
     5221 gacaacaaac cccaatggtg ccactctgct ccaacctgca gctccatcct atgcttctca
     5281 cagacaacgt ggttcctact gtcagatcac atttggggcg ggaagggtgg ttttttaaaa
     5341 aaactcgttt ttatgagcag gctatcttga tcaatagcaa acttttttta atggattagt
     5401 gaaataaatg tccccatgca tcatttatag tctgtttctt tctccgtgtg gaatcaagat
     5461 aagactgcca gcactagacg gtttctgtta gctgctgaga ccagggcctc accttgctca
     5521 agctagtggg tgaatttgca accagctaat actcttcctt ggtttgtttc cactttttat
     5581 ctgttggttg ttggctttct tttccattct gtattttcta tctgactctg tatactgtat
     5641 aaagcagttt tttcttgtct gtttatcttt cttcaactgg aatatttaca ataaaacaga
     5701 aacaaatcct gaaatgttag tgatggatga cagctgtttg ggtttagaaa gtatggttca
     5761 gtgtgtctaa tatccaggta ctagacttga aaagcaaata aaatcataaa cccac
//
