LOCUS       NM_001077243            5619 bp    mRNA    linear   PRI 22-SEP-2024
DEFINITION  Homo sapiens glutamate ionotropic receptor AMPA type subunit 4
            (GRIA4), transcript variant 2, mRNA.
ACCESSION   NM_001077243
VERSION     NM_001077243.3
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5619)
  AUTHORS   Justo-Garrido,M., Lopez-Saavedra,A., Alcaraz,N.,
            Cortes-Gonzalez,C.C., Onate-Ocana,L.F., Caro-Sanchez,C.H.S.,
            Castro-Hernandez,C., Arriaga-Canon,C., Diaz-Chavez,J. and
            Herrera,L.A.
  TITLE     Association of SLC12A1 and GLUR4 Ion Transporters with Neoadjuvant
            Chemoresistance in Luminal Locally Advanced Breast Cancer
  JOURNAL   Int J Mol Sci 24 (22), 16104 (2023)
   PUBMED   38003293
  REMARK    GeneRIF: Association of SLC12A1 and GLUR4 Ion Transporters with
            Neoadjuvant Chemoresistance in Luminal Locally Advanced Breast
            Cancer.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5619)
  AUTHORS   Vega-Benedetti,A.F., Loi,E., Moi,L., Restivo,A., Cabras,F.,
            Deidda,S., Pretta,A., Ziranu,P., Orru,S., Scartozzi,M., Zorcolo,L.
            and Zavattari,P.
  TITLE     Colorectal cancer promoter methylation alteration affects the
            expression of glutamate ionotropic receptor AMPA type subunit 4
            alternative isoforms potentially relevant in colon tissue
  JOURNAL   Hum Cell 35 (1), 310-319 (2022)
   PUBMED   34719006
  REMARK    GeneRIF: Colorectal cancer promoter methylation alteration affects
            the expression of glutamate ionotropic receptor AMPA type subunit 4
            alternative isoforms potentially relevant in colon tissue.
REFERENCE   3  (bases 1 to 5619)
  AUTHORS   Herbrechter,R., Hube,N., Buchholz,R. and Reiner,A.
  TITLE     Splicing and editing of ionotropic glutamate receptors: a
            comprehensive analysis based on human RNA-Seq data
  JOURNAL   Cell Mol Life Sci 78 (14), 5605-5630 (2021)
   PUBMED   34100982
REFERENCE   4  (bases 1 to 5619)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   5  (bases 1 to 5619)
  AUTHORS   Aruscavage,P.J. and Bass,B.L.
  TITLE     A phylogenetic analysis reveals an unusual sequence conservation
            within introns involved in RNA editing
  JOURNAL   RNA 6 (2), 257-269 (2000)
   PUBMED   10688364
REFERENCE   6  (bases 1 to 5619)
  AUTHORS   Fletcher,E.J., Nutt,S.L., Hoo,K.H., Elliott,C.E., Korczak,B.,
            McWhinnie,E.A. and Kamboj,R.K.
  TITLE     Cloning, expression and pharmacological characterization of a human
            glutamate receptor: hGluR4
  JOURNAL   Recept Channels 3 (1), 21-31 (1995)
   PUBMED   8589990
REFERENCE   7  (bases 1 to 5619)
  AUTHORS   Lomeli,H., Mosbacher,J., Melcher,T., Hoger,T., Geiger,J.R.,
            Kuner,T., Monyer,H., Higuchi,M., Bach,A. and Seeburg,P.H.
  TITLE     Control of kinetic properties of AMPA receptor channels by nuclear
            RNA editing
  JOURNAL   Science 266 (5191), 1709-1713 (1994)
   PUBMED   7992055
REFERENCE   8  (bases 1 to 5619)
  AUTHORS   Hardy,M., Younkin,D., Tang,C.M., Pleasure,J., Shi,Q.Y., Williams,M.
            and Pleasure,D.
  TITLE     Expression of non-NMDA glutamate receptor channel genes by clonal
            human neurons
  JOURNAL   J Neurochem 63 (2), 482-489 (1994)
   PUBMED   7518497
REFERENCE   9  (bases 1 to 5619)
  AUTHORS   Roche,K.W., Raymond,L.A., Blackstone,C. and Huganir,R.L.
  TITLE     Transmembrane topology of the glutamate receptor subunit GluR6
  JOURNAL   J Biol Chem 269 (16), 11679-11682 (1994)
   PUBMED   8163463
REFERENCE   10 (bases 1 to 5619)
  AUTHORS   McNamara,J.O., Eubanks,J.H., McPherson,J.D., Wasmuth,J.J.,
            Evans,G.A. and Heinemann,S.F.
  TITLE     Chromosomal localization of human glutamate receptor genes
  JOURNAL   J Neurosci 12 (7), 2555-2562 (1992)
   PUBMED   1319477
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AP001561.4, AP000641.4,
            AP000673.4 and AP000813.4.
            
            On Aug 14, 2020 this sequence version replaced NM_001077243.2.
            
            Summary: Glutamate receptors are the predominant excitatory
            neurotransmitter receptors in the mammalian brain and are activated
            in a variety of normal neurophysiologic processes. These receptors
            are heteromeric protein complexes composed of multiple subunits,
            arranged to form ligand-gated ion channels. The classification of
            glutamate receptors is based on their activation by different
            pharmacologic agonists. The subunit encoded by this gene belongs to
            a family of AMPA (alpha-amino-3-hydroxy-5-methyl-4-isoxazole
            propionate)-sensitive glutamate receptors, and is subject to RNA
            editing (AGA->GGA; R->G). Alternative splicing of this gene results
            in transcript variants encoding different isoforms, which may vary
            in their signal transduction properties. Some haplotypes of this
            gene show a positive association with schizophrenia. [provided by
            RefSeq, Jul 2008].
            
            Transcript Variant: This variant (2) contains an alternate in-frame
            coding exon, and uses a downstream donor splice site at the
            penultimate exon, compared to transcript variant 1. This results in
            a shorter isoform (2) with a different C-terminus compared to
            isoform 1. RNA editing (AGA->GGA) changes Arg765Gly.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data because no single transcript was available
            for the full length of the gene. The extent of this transcript is
            supported by transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660807.685.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2145743, SAMEA2146411
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            undergoes RNA editing :: PMID: 10688364, 7992055
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-356               AP001561.4         80434-80789
            357-534             AP001561.4         81269-81446
            535-693             AP001561.4         82637-82795
            694-933             AP000641.4         48462-48701
            934-1118            AP000673.4         68522-68706
            1119-1172           AP000673.4         94017-94070
            1173-1331           AP000673.4         104767-104925
            1332-1499           AP000673.4         110312-110479
            1500-1604           AP000673.4         111695-111799
            1605-1715           AP000673.4         116933-117043
            1716-1922           AP000673.4         125210-125416
            1923-2293           AP000673.4         130897-131267
            2294-2492           AP000673.4         133239-133437
            2493-2740           AP000673.4         140220-140467
            2741-2855           AP000813.4         24268-24382
            2856-3103           AP000813.4         32631-32878
            3104-5619           AP000813.4         37896-40411
FEATURES             Location/Qualifiers
     source          1..5619
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11q22.3"
     gene            1..5619
                     /gene="GRIA4"
                     /gene_synonym="GluA4; GluA4-ATD; GLUR4; GLUR4C; GLURD;
                     NEDSGA"
                     /note="glutamate ionotropic receptor AMPA type subunit 4"
                     /db_xref="GeneID:2893"
                     /db_xref="HGNC:HGNC:4574"
                     /db_xref="MIM:138246"
     exon            1..356
                     /gene="GRIA4"
                     /gene_synonym="GluA4; GluA4-ATD; GLUR4; GLUR4C; GLURD;
                     NEDSGA"
                     /inference="alignment:Splign:2.1.0"
     exon            357..534
                     /gene="GRIA4"
                     /gene_synonym="GluA4; GluA4-ATD; GLUR4; GLUR4C; GLURD;
                     NEDSGA"
                     /inference="alignment:Splign:2.1.0"
     CDS             447..3101
                     /gene="GRIA4"
                     /gene_synonym="GluA4; GluA4-ATD; GLUR4; GLUR4C; GLURD;
                     NEDSGA"
                     /note="isoform 2 precursor is encoded by transcript
                     variant 2; glutamate receptor 4; AMPA-selective glutamate
                     receptor 4; gluR-4; gluR-D; glutamate receptor,
                     ionotrophic, AMPA 4; glutamate receptor, ionotropic, AMPA
                     4"
                     /codon_start=1
                     /product="glutamate receptor 4 isoform 2 precursor"
                     /protein_id="NP_001070711.3"
                     /db_xref="CCDS:CCDS41706.1"
                     /db_xref="GeneID:2893"
                     /db_xref="HGNC:HGNC:4574"
                     /db_xref="MIM:138246"
                     /translation="MRIISRQIVLLFSGFWGLAMGAFPSSVQIGGLFIRNTDQEYTAF
                     RLAIFLHNTSPNASEAPFNLVPHVDNIETANSFAVTNAFCSQYSRGVFAIFGLYDKRS
                     VHTLTSFCSALHISLITPSFPTEGESQFVLQLRPSLRGALLSLLDHYEWNCFVFLYDT
                     DRGYSILQAIMEKAGQNGWHVSAICVENFNDVSYRQLLEELDRRQEKKFVIDCEIERL
                     QNILEQIVSVGKHVKGYHYIIANLGFKDISLERFIHGGANVTGFQLVDFNTPMVIKLM
                     DRWKKLDQREYPGSETPPKYTSALTYDGVLVMAETFRSLRRQKIDISRRGNAGDCLAN
                     PAAPWGQGIDMERTLKQVRIQGLTGNVQFDHYGRRVNYTMDVFELKSTGPRKVGYWND
                     MDKLVLIQDVPTLGNDTAAIENRTVVVTTIMESPYVMYKKNHEMFEGNDKYEGYCVDL
                     ASEIAKHIGIKYKIAIVPDGKYGARDADTKIWNGMVGELVYGKAEIAIAPLTITLVRE
                     EVIDFSKPFMSLGISIMIKKPQKSKPGVFSFLDPLAYEIWMCIVFAYIGVSVVLFLVS
                     RFSPYEWHTEEPEDGKEGPSDQPPNEFGIFNSLWFSLGAFMQQGCDISPRSLSGRIVG
                     GVWWFFTLIIISSYTANLAAFLTVERMVSPIESAEDLAKQTEIAYGTLDSGSTKEFFR
                     RSKIAVYEKMWTYMRSAEPSVFTRTTAEGVARVRKSKGKFAFLLESTMNEYIEQRKPC
                     DTMKVGGNLDSKGYGVATPKGSSLRNAVNLAVLKLNEQGLLDKLKNKWWYDKGECGSG
                     GGDSKDKTSALSLSNVAGVFYILVGGLGLAMLVALIEFCYKSRAEAKRMKVAKSAQTF
                     NPTSSQNTQNLATYREGYNVYGTESIKI"
     exon            535..693
                     /gene="GRIA4"
                     /gene_synonym="GluA4; GluA4-ATD; GLUR4; GLUR4C; GLURD;
                     NEDSGA"
                     /inference="alignment:Splign:2.1.0"
     exon            694..933
                     /gene="GRIA4"
                     /gene_synonym="GluA4; GluA4-ATD; GLUR4; GLUR4C; GLURD;
                     NEDSGA"
                     /inference="alignment:Splign:2.1.0"
     exon            934..1118
                     /gene="GRIA4"
                     /gene_synonym="GluA4; GluA4-ATD; GLUR4; GLUR4C; GLURD;
                     NEDSGA"
                     /inference="alignment:Splign:2.1.0"
     exon            1119..1172
                     /gene="GRIA4"
                     /gene_synonym="GluA4; GluA4-ATD; GLUR4; GLUR4C; GLURD;
                     NEDSGA"
                     /inference="alignment:Splign:2.1.0"
     exon            1173..1331
                     /gene="GRIA4"
                     /gene_synonym="GluA4; GluA4-ATD; GLUR4; GLUR4C; GLURD;
                     NEDSGA"
                     /inference="alignment:Splign:2.1.0"
     exon            1332..1499
                     /gene="GRIA4"
                     /gene_synonym="GluA4; GluA4-ATD; GLUR4; GLUR4C; GLURD;
                     NEDSGA"
                     /inference="alignment:Splign:2.1.0"
     exon            1500..1604
                     /gene="GRIA4"
                     /gene_synonym="GluA4; GluA4-ATD; GLUR4; GLUR4C; GLURD;
                     NEDSGA"
                     /inference="alignment:Splign:2.1.0"
     exon            1605..1715
                     /gene="GRIA4"
                     /gene_synonym="GluA4; GluA4-ATD; GLUR4; GLUR4C; GLURD;
                     NEDSGA"
                     /inference="alignment:Splign:2.1.0"
     exon            1716..1922
                     /gene="GRIA4"
                     /gene_synonym="GluA4; GluA4-ATD; GLUR4; GLUR4C; GLURD;
                     NEDSGA"
                     /inference="alignment:Splign:2.1.0"
     exon            1923..2293
                     /gene="GRIA4"
                     /gene_synonym="GluA4; GluA4-ATD; GLUR4; GLUR4C; GLURD;
                     NEDSGA"
                     /inference="alignment:Splign:2.1.0"
     exon            2294..2492
                     /gene="GRIA4"
                     /gene_synonym="GluA4; GluA4-ATD; GLUR4; GLUR4C; GLURD;
                     NEDSGA"
                     /inference="alignment:Splign:2.1.0"
     exon            2493..2740
                     /gene="GRIA4"
                     /gene_synonym="GluA4; GluA4-ATD; GLUR4; GLUR4C; GLURD;
                     NEDSGA"
                     /inference="alignment:Splign:2.1.0"
     exon            2741..2855
                     /gene="GRIA4"
                     /gene_synonym="GluA4; GluA4-ATD; GLUR4; GLUR4C; GLURD;
                     NEDSGA"
                     /inference="alignment:Splign:2.1.0"
     exon            2856..3103
                     /gene="GRIA4"
                     /gene_synonym="GluA4; GluA4-ATD; GLUR4; GLUR4C; GLURD;
                     NEDSGA"
                     /inference="alignment:Splign:2.1.0"
     exon            3104..5619
                     /gene="GRIA4"
                     /gene_synonym="GluA4; GluA4-ATD; GLUR4; GLUR4C; GLURD;
                     NEDSGA"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5599..5604
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GRIA4"
                     /gene_synonym="GluA4; GluA4-ATD; GLUR4; GLUR4C; GLURD;
                     NEDSGA"
                     /note="hexamer: AATAAA"
     polyA_site      5619
                     /gene="GRIA4"
                     /gene_synonym="GluA4; GluA4-ATD; GLUR4; GLUR4C; GLURD;
                     NEDSGA"
                     /note="major polyA site"
ORIGIN      
        1 agtggcagaa gagggctagg ctgagaggga agccaggact gtaggagagg gaggcagccc
       61 gtcctcctca cgaacctgca aggatgcggc aggggcctgg gggcatgggg aggtactaac
      121 cccccggagc ccccgattgg ggcttgcaga cctggcccgt gggcggattt tctgcctagc
      181 gcagccgaga agcagaggtg ccaggaaaac caagagaggg gcgctggggg tgcccatccc
      241 cagagtcggt ccctctgcga accgaggaag aaaagaggag ggagtcagcg agtggtcaga
      301 agggaaaacc tgacaccaga ctggctccgg agcgtccggg agactggggc gctccgcgcc
      361 atcgtcttca atgcttctct gaacagcctt taggaagagt gcgagagaaa gagagagagc
      421 gcgcgccagg gagaggagaa aagaagatga ggattatttc cagacagatt gtcttgttat
      481 tttctggatt ttggggactc gccatgggag cctttccgag cagcgtgcaa ataggtggtc
      541 tcttcatccg aaacacagat caggaataca ctgcttttcg attagcaatt tttcttcata
      601 acaccagccc caatgcgtcg gaagctcctt ttaatttggt acctcatgtg gacaacattg
      661 agacagccaa cagttttgct gtaacaaacg ccttctgttc ccagtattct agaggagtat
      721 ttgccatttt tggactctat gataagaggt cggtacatac cttgacctca ttctgcagcg
      781 ccttacatat ctccctcatc acaccaagtt tccctactga gggggagagc cagtttgtgc
      841 tgcaactaag accttcgtta cgaggagcac tcttgagttt gctggatcac tacgaatgga
      901 actgttttgt cttcctgtat gacacagaca ggggatactc gatactccaa gctattatgg
      961 aaaaagcagg acaaaatggt tggcatgtca gcgctatatg tgtggaaaat tttaatgatg
     1021 tcagctatag gcaacttcta gaagaacttg acagaagaca agagaagaag tttgtaatag
     1081 actgtgagat agagagactt caaaacatat tagaacagat tgtaagtgtt ggaaagcatg
     1141 ttaaaggcta ccattatatc attgcaaact tgggattcaa ggatatttct cttgagaggt
     1201 ttatacatgg tggagccaat gttactggat tccagttggt ggattttaat acacctatgg
     1261 taatcaaact aatggatcgc tggaagaaac tagatcagag agagtatcca ggatctgaga
     1321 ctcctccaaa gtacacctct gctctgactt atgatggagt ccttgtgatg gctgaaactt
     1381 tccgaagtct taggaggcag aaaattgata tctcaaggag aggaaatgct ggggattgtc
     1441 tggcaaatcc tgctgctcca tggggccagg gaattgacat ggagaggaca ctcaaacagg
     1501 ttcgaattca agggctgaca gggaatgttc agtttgacca ctatggacgt agagtcaatt
     1561 acacaatgga tgtgtttgag ctgaaaagca caggacctag aaaggttggt tactggaatg
     1621 atatggataa gttagtcttg attcaagatg taccaactct tggcaatgac acagctgcta
     1681 ttgagaacag aacagtggtt gtaaccacaa ttatggaatc cccatatgtt atgtacaaga
     1741 aaaatcatga aatgtttgaa ggaaatgaca agtatgaagg atactgtgta gatttggcat
     1801 ctgaaattgc aaaacatatt ggtatcaagt ataaaattgc cattgtccct gatggaaaat
     1861 atggagcaag ggatgcagac acaaaaatct ggaatgggat ggtaggagaa cttgtttatg
     1921 ggaaagcaga gattgctatt gcccctctga caatcacttt ggtacgagag gaggtcattg
     1981 acttttctaa gcccttcatg agtttgggca tatctatcat gatcaaaaag cctcagaaat
     2041 ccaaaccagg agtgttttcc ttcttggatc ctctggccta tgagatttgg atgtgcatag
     2101 tctttgccta cattggtgtc agcgtggtct tattcctagt tagtagattt agtccatatg
     2161 agtggcacac agaagagcca gaggacggaa aggaaggacc cagcgaccag cctcccaatg
     2221 agtttggcat ctttaacagc ctctggtttt ccctgggtgc ttttatgcag caaggatgtg
     2281 acatttcacc cagatccctc tcaggtcgaa ttgttggagg tgtttggtgg ttctttacac
     2341 tcatcattat atcatcttat actgctaacc tcgctgcttt cctgacggtt gagcgaatgg
     2401 tctctcccat agaaagtgca gaagacctgg ccaaacaaac agaaattgcc tatggaacac
     2461 tggattcagg atcaacaaaa gaattcttca gaagatcaaa aatagcagtg tatgaaaaga
     2521 tgtggaccta catgcgatca gcagagccat cagtattcac taggactaca gctgagggag
     2581 tagctcgtgt ccgcaaatcc aagggcaaat ttgcctttct cctggagtcc actatgaatg
     2641 aatacattga gcagcgaaag ccatgtgaca cgatgaaagt gggaggaaat ctggattcca
     2701 aaggctatgg agtagcaacg cccaagggtt cctcattaag aaatgctgtt aacctcgcag
     2761 ttttaaaact gaatgaacaa ggcctcttgg acaaattgaa aaacaaatgg tggtacgaca
     2821 aaggagaatg tggcagcggg ggaggtgact ccaaggacaa gacgagtgcc ttgagcctga
     2881 gcaatgtagc aggcgtcttc tacattctgg ttggcggctt gggcttggca atgctggtgg
     2941 ctttgataga gttctgttac aagtccaggg cagaagcgaa gagaatgaag gtggcaaaga
     3001 gtgcacagac ttttaaccca acttcctcgc agaataccca gaatttagca acctatagag
     3061 aaggttacaa cgtatatgga accgaaagta ttaaaattta gggctgacct tttctgaagc
     3121 cataagaaac aaagccagat tatccatcac tgggagtgtg ggagagaatg gccgcgtctt
     3181 gacgcctgac tgcccaaagg ctgtacacac tggaactgca atcagacaaa gttcaggatt
     3241 ggctgtcatt gcatcggacc taccataaaa accaaaaaaa taattgagtg ccttaattaa
     3301 actgttggtg actggtggaa acgcagccct gagggacacg ccacgcgcgg gtctttgcta
     3361 aaccaatcct ttggctgaga gcgggaagtc cgtcctaacg cgctggccgg acatcagcag
     3421 cagcaacgtg tgcatgagct cagctcggaa acccaaactc agattttata tcaggaaaac
     3481 tcacaattga ggtttttttc ggggagtggg tgggggaggg atctgggatg ggtgtattaa
     3541 cagcaacaaa tttcattcga gtggactcaa aaactaatca gacttatgag ttagcgcatt
     3601 aaactgtgaa gttcttgctc agaaaggcct ttgtcttcac cggaaaggat aaaatagttg
     3661 tagaagtccg tgaacatgct aacctgtgtc tccagaacat ccatatagtc catggaagaa
     3721 aatccagctg agaaaacaaa tcactaaact gtgataagaa aataatgaac aaacatgtaa
     3781 aacctgtggg aaaaaaaaat aaaggaagta tgtacactta ctttggagaa aacaaatact
     3841 gaaacatgct tgctttttaa ctgacgtaaa ttcagtagag gacaacacaa ttcttttttc
     3901 taaccatctt agggaacaat acattgcaat aattgatata aatgccatca ctgtaataaa
     3961 ctttagagac ttttttttat aaaagttgtt ggtcatcttc ttgtttgctg taaccttcac
     4021 tatgtcacat gagtcgattc accgattgca tttgtctcac aaccaggaag aaaagcaaaa
     4081 ggaagaaaac gtttaggttc aatcatcagt ctgcggtgta gactcgaaag agatgacagg
     4141 tcactcatgt taatggtatt atttataatc tcattctgtg tacaacattg tggtttttgt
     4201 acccaccaaa aagaataaaa cagcagatgt tcttacaata tctacagagc ttaaaagttt
     4261 tttcttatcg ttataaaagt tatttgagaa attataagac tataagagag attgtattag
     4321 tggtgggcca tagtggaaaa tgtagctagc cctcattatt ttttgcatac taagctaccc
     4381 ctccttttca gatctttgac tcattaacag attaaactgt caaagatgga gtctttgagt
     4441 tggggaatga atcactgtcc taacaacaac ataccttgta attgtgtgtt gaaattttac
     4501 ttgactgtat tttgctgcat aaaattatgt gtctcttggg cttcttccct tattcctatt
     4561 gttcccttta aatcatatga aggcattcat aatagcttgg ggtagataac aaatgaagaa
     4621 ttagtctttg ttttcaactg gaaattgtaa agaaaattat actcatgttt atttataaaa
     4681 atcaccttat gtatgaatta aactaacatg gttcaaaaga aggtttggtt catttgaaat
     4741 aataaataag tactctaata cagataaaaa tcatgtactt agggtattgg cagaaagcac
     4801 aagttaggat gatttcagaa gtctggcctt gaaggatgag ttgagtttta acaggaggag
     4861 aaggtgttaa gagccatatg agtgagcagt ggcccaaagc catgcacatc agtggctcat
     4921 ttaaggaatg aatgccatta gatgggctac tgagagtaca gggatattat ggaagataaa
     4981 gttggaaaag ctgaaggatt gattttcttc catcaactct caagatccca ttcgccattc
     5041 aatctctgtg ctgcagtaag agcaatctta aacagtataa atcacacaca cacacacaca
     5101 cacacacaca cacacacaca agtccctcag gaaaaattcc aagctcttga gaagatcaca
     5161 tgagcccctt catgacctgg cgcttgctta tttcttccag gacttctctc acttctatcc
     5221 agctattccc gtcagcaaat gaacctccaa agcagcacat ggagcactgc atagactatt
     5281 tcctcagtgc gtaactcctc cctgtctcct ctttacctga gtaacttgta ctcatccttc
     5341 aatactccaa ctgaatttta cttaccctga aaagatttcc atggctatcc accacccccc
     5401 tgcctgtgag actgagttag gtgccctttt tcatgtcttt cccccatcac ggcacttacc
     5461 atactgcgtt gtaattgcct gtgtactcgt ctgtataact actagactgt aagctccttg
     5521 agggcaggga ctgtgtctat cttgttcaca gttgtatccc cagcacccag cacagtgcct
     5581 ggcatattgt aggtgcttaa taaatatttg ttgaatgaa
//
