LOCUS       NM_007325               5148 bp    mRNA    linear   PRI 06-DEC-2024
DEFINITION  Homo sapiens glutamate ionotropic receptor AMPA type subunit 3
            (GRIA3), transcript variant 1, mRNA.
ACCESSION   NM_007325
VERSION     NM_007325.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5148)
  AUTHORS   Rinaldi,B., Bayat,A., Zachariassen,L.G., Sun,J.H., Ge,Y.H.,
            Zhao,D., Bonde,K., Madsen,L.H., Awad,I.A.A., Bagiran,D., Sbeih,A.,
            Shah,S.M., El-Sayed,S., Lyngby,S.M., Pedersen,M.G., Stenum-Berg,C.,
            Walker,L.C., Krey,I., Delahaye-Duriez,A., Emrick,L.T., Sully,K.,
            Murali,C.N., Burrage,L.C., Plaud Gonzalez,J.A., Parnes,M.,
            Friedman,J., Isidor,B., Lefranc,J., Redon,S., Heron,D., Mignot,C.,
            Keren,B., Fradin,M., Dubourg,C., Mercier,S., Besnard,T., Cogne,B.,
            Deb,W., Rivier,C., Milani,D., Bedeschi,M.F., Di Napoli,C.,
            Grilli,F., Marchisio,P., Koudijs,S., Veenma,D., Argilli,E.,
            Lynch,S.A., Au,P.Y.B., Ayala Valenzuela,F.E., Brown,C.,
            Masser-Frye,D., Jones,M., Patron Romero,L., Li,W.L., Thorpe,E.,
            Hecher,L., Johannsen,J., Denecke,J., McNiven,V., Szuto,A.,
            Wakeling,E., Cruz,V., Sency,V., Wang,H., Piard,J., Kortum,F.,
            Herget,T., Bierhals,T., Condell,A., Ben-Zeev,B., Kaur,S.,
            Christodoulou,J., Piton,A., Zweier,C., Kraus,C., Micalizzi,A.,
            Trivisano,M., Specchio,N., Lesca,G., Moller,R.S., Tumer,Z.,
            Musgaard,M., Gerard,B., Lemke,J.R., Shi,Y.S. and Kristensen,A.S.
  TITLE     Gain-of-function and loss-of-function variants in GRIA3 lead to
            distinct neurodevelopmental phenotypes
  JOURNAL   Brain 147 (5), 1837-1855 (2024)
   PUBMED   38038360
  REMARK    GeneRIF: Gain-of-function and loss-of-function variants in GRIA3
            lead to distinct neurodevelopmental phenotypes.
REFERENCE   2  (bases 1 to 5148)
  AUTHORS   Peng,S.X., Pei,J., Rinaldi,B., Chen,J., Ge,Y.H., Jia,M., Wang,J.,
            Delahaye-Duriez,A., Sun,J.H., Zang,Y.Y., Shi,Y.Y., Zhang,N.,
            Gao,X., Milani,D., Xu,X., Sheng,N., Gerard,B., Zhang,C., Bayat,A.,
            Liu,N., Yang,J.J. and Shi,Y.S.
  TITLE     Dysfunction of AMPA receptor GluA3 is associated with aggressive
            behavior in human
  JOURNAL   Mol Psychiatry 27 (10), 4092-4102 (2022)
   PUBMED   35697757
  REMARK    GeneRIF: Dysfunction of AMPA receptor GluA3 is associated with
            aggressive behavior in human.
REFERENCE   3  (bases 1 to 5148)
  AUTHORS   Hamanaka,K., Miyoshi,K., Sun,J.H., Hamada,K., Komatsubara,T.,
            Saida,K., Tsuchida,N., Uchiyama,Y., Fujita,A., Mizuguchi,T.,
            Gerard,B., Bayat,A., Rinaldi,B., Kato,M., Tohyama,J., Ogata,K.,
            Shi,Y.S., Saito,K., Miyatake,S. and Matsumoto,N.
  TITLE     Amelioration of a neurodevelopmental disorder by carbamazepine in a
            case having a gain-of-function GRIA3 variant
  JOURNAL   Hum Genet 141 (2), 283-293 (2022)
   PUBMED   35031858
  REMARK    GeneRIF: Amelioration of a neurodevelopmental disorder by
            carbamazepine in a case having a gain-of-function GRIA3 variant.
REFERENCE   4  (bases 1 to 5148)
  AUTHORS   Hu,M.J., Long,M. and Dai,R.J.
  TITLE     Acetylation of H3K27 activated lncRNA NEAT1 and promoted hepatic
            lipid accumulation in non-alcoholic fatty liver disease via
            regulating miR-212-5p/GRIA3
  JOURNAL   Mol Cell Biochem 477 (1), 191-203 (2022)
   PUBMED   34652536
  REMARK    GeneRIF: Acetylation of H3K27 activated lncRNA NEAT1 and promoted
            hepatic lipid accumulation in non-alcoholic fatty liver disease via
            regulating miR-212-5p/GRIA3.
REFERENCE   5  (bases 1 to 5148)
  AUTHORS   Sun,J.H., Chen,J., Ayala Valenzuela,F.E., Brown,C., Masser-Frye,D.,
            Jones,M., Romero,L.P., Rinaldi,B., Li,W.L., Li,Q.Q., Wu,D.,
            Gerard,B., Thorpe,E., Bayat,A. and Shi,Y.S.
  TITLE     X-linked neonatal-onset epileptic encephalopathy associated with a
            gain-of-function variant p.R660T in GRIA3
  JOURNAL   PLoS Genet 17 (6), e1009608 (2021)
   PUBMED   34161333
  REMARK    GeneRIF: X-linked neonatal-onset epileptic encephalopathy
            associated with a gain-of-function variant p.R660T in GRIA3.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 5148)
  AUTHORS   Tomiyama,M., Rodriguez-Puertas,R., Cortes,R., Christnacher,A.,
            Sommer,B., Pazos,A., Palacios,J.M. and Mengod,G.
  TITLE     Differential regional distribution of AMPA receptor subunit
            messenger RNAs in the human spinal cord as visualized by in situ
            hybridization
  JOURNAL   Neuroscience 75 (3), 901-915 (1996)
   PUBMED   8951883
REFERENCE   7  (bases 1 to 5148)
  AUTHORS   Rampersad,V., Elliott,C.E., Nutt,S.L., Foldes,R.L. and Kamboj,R.K.
  TITLE     Human glutamate receptor hGluR3 flip and flop isoforms: cloning and
            sequencing of the cDNAs and primary structure of the proteins
  JOURNAL   Biochim Biophys Acta 1219 (2), 563-566 (1994)
   PUBMED   7918660
REFERENCE   8  (bases 1 to 5148)
  AUTHORS   Rogers,S.W., Andrews,P.I., Gahring,L.C., Whisenand,T., Cauley,K.,
            Crain,B., Hughes,T.E., Heinemann,S.F. and McNamara,J.O.
  TITLE     Autoantibodies to glutamate receptor GluR3 in Rasmussen's
            encephalitis
  JOURNAL   Science 265 (5172), 648-651 (1994)
   PUBMED   8036512
REFERENCE   9  (bases 1 to 5148)
  AUTHORS   McNamara,J.O., Eubanks,J.H., McPherson,J.D., Wasmuth,J.J.,
            Evans,G.A. and Heinemann,S.F.
  TITLE     Chromosomal localization of human glutamate receptor genes
  JOURNAL   J Neurosci 12 (7), 2555-2562 (1992)
   PUBMED   1319477
REFERENCE   10 (bases 1 to 5148)
  AUTHORS   Hollmann,M., Hartley,M. and Heinemann,S.
  TITLE     Ca2+ permeability of KA-AMPA--gated glutamate receptor channels
            depends on subunit composition
  JOURNAL   Science 252 (5007), 851-853 (1991)
   PUBMED   1709304
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from Z83848.3, AL035426.2, Z82899.2,
            AL356213.10 and AL590139.11.
            This sequence is a reference standard in the RefSeqGene project.
            
            On May 17, 2019 this sequence version replaced NM_007325.4.
            
            Summary: Glutamate receptors are the predominant excitatory
            neurotransmitter receptors in the mammalian brain and are activated
            in a variety of normal neurophysiologic processes. These receptors
            are heteromeric protein complexes composed of multiple subunits,
            arranged to form ligand-gated ion channels. The classification of
            glutamate receptors is based on their activation by different
            pharmacologic agonists. The subunit encoded by this gene belongs to
            a family of AMPA (alpha-amino-3-hydroxy-5-methyl-4-isoxazole
            propionate)-sensitive glutamate receptors, and is subject to RNA
            editing (AGA->GGA; R->G). Alternative splicing at this locus
            results in different isoforms, which may vary in their signal
            transduction properties. [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) encodes isoform 1 (also known
            as flip isoform). RNA editing (AGA->GGA) changes Arg775Gly.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803617.37697.1,
                                           SRR1803612.141623.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968540, SAMEA2145544
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000620443.2/ ENSP00000478489.1
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            undergoes RNA editing  :: PMID: 10688364, 7992055
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-367               Z83848.3           66695-67061         c
            368-526             Z83848.3           65349-65507         c
            527-766             AL035426.2         2065-2304
            767-954             Z82899.2           57012-57199         c
            955-1008            Z82899.2           28262-28315         c
            1009-1170           AL356213.10        11844-12005
            1171-1338           AL356213.10        15512-15679
            1339-1443           AL356213.10        19870-19974
            1444-1551           AL356213.10        20288-20395
            1552-1758           AL356213.10        21584-21790
            1759-2135           AL356213.10        34278-34654
            2136-2334           AL356213.10        44817-45015
            2335-2582           AL356213.10        81741-81988
            2583-2697           AL590139.11        11146-11260
            2698-2945           AL590139.11        13882-14129
            2946-5148           AL590139.11        19796-21998
FEATURES             Location/Qualifiers
     source          1..5148
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="X"
                     /map="Xq25"
     gene            1..5148
                     /gene="GRIA3"
                     /gene_synonym="GluA3; GLUR-C; GLUR-K3; GLUR3; GLURC;
                     iGluR3; MRX94; MRXSW"
                     /note="glutamate ionotropic receptor AMPA type subunit 3"
                     /db_xref="GeneID:2892"
                     /db_xref="HGNC:HGNC:4573"
                     /db_xref="MIM:305915"
     exon            1..367
                     /gene="GRIA3"
                     /gene_synonym="GluA3; GLUR-C; GLUR-K3; GLUR3; GLURC;
                     iGluR3; MRX94; MRXSW"
                     /inference="alignment:Splign:2.1.0"
     CDS             259..2943
                     /gene="GRIA3"
                     /gene_synonym="GluA3; GLUR-C; GLUR-K3; GLUR3; GLURC;
                     iGluR3; MRX94; MRXSW"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; glutamate receptor subunit 3; AMPA receptor
                     subunit GluA3; dJ1171F9.1; glutamate receptor C; gluR-3;
                     AMPA-selective glutamate receptor 3; glutamate receptor,
                     ionotrophic, AMPA 3; glutamate receptor, ionotropic, AMPA
                     3"
                     /codon_start=1
                     /product="glutamate receptor 3 isoform 1 precursor"
                     /protein_id="NP_015564.5"
                     /db_xref="CCDS:CCDS14605.1"
                     /db_xref="GeneID:2892"
                     /db_xref="HGNC:HGNC:4573"
                     /db_xref="MIM:305915"
                     /translation="MARQKKMGQSVLRAVFFLVLGLLGHSHGGFPNTISIGGLFMRNT
                     VQEHSAFRFAVQLYNTNQNTTEKPFHLNYHVDHLDSSNSFSVTNAFCSQFSRGVYAIF
                     GFYDQMSMNTLTSFCGALHTSFVTPSFPTDADVQFVIQMRPALKGAILSLLGHYKWEK
                     FVYLYDTERGFSILQAIMEAAVQNNWQVTARSVGNIKDVQEFRRIIEEMDRRQEKRYL
                     IDCEVERINTILEQVVILGKHSRGYHYMLANLGFTDILLERVMHGGANITGFQIVNNE
                     NPMVQQFIQRWVRLDEREFPEAKNAPLKYTSALTHDAILVIAEAFRYLRRQRVDVSRR
                     GSAGDCLANPAVPWSQGIDIERALKMVQVQGMTGNIQFDTYGRRTNYTIDVYEMKVSG
                     SRKAGYWNEYERFVPFSDQQISNDSASSENRTIVVTTILESPYVMYKKNHEQLEGNER
                     YEGYCVDLAYEIAKHVRIKYKLSIVGDGKYGARDPETKIWNGMVGELVYGRADIAVAP
                     LTITLVREEVIDFSKPFMSLGISIMIKKPQKSKPGVFSFLDPLAYEIWMCIVFAYIGV
                     SVVLFLVSRFSPYEWHLEDNNEEPRDPQSPPDPPNEFGIFNSLWFSLGAFMQQGCDIS
                     PRSLSGRIVGGVWWFFTLIIISSYTANLAAFLTVERMVSPIESAEDLAKQTEIAYGTL
                     DSGSTKEFFRRSKIAVYEKMWSYMKSAEPSVFTKTTADGVARVRKSKGKFAFLLESTM
                     NEYIEQRKPCDTMKVGGNLDSKGYGVATPKGSALRTPVNLAVLKLSEQGILDKLKNKW
                     WYDKGECGAKDSGSKDKTSALSLSNVAGVFYILVGGLGLAMMVALIEFCYKSRAESKR
                     MKLTKNTQNFKPAPATNTQNYATYREGYNVYGTESVKI"
     exon            368..526
                     /gene="GRIA3"
                     /gene_synonym="GluA3; GLUR-C; GLUR-K3; GLUR3; GLURC;
                     iGluR3; MRX94; MRXSW"
                     /inference="alignment:Splign:2.1.0"
     exon            527..766
                     /gene="GRIA3"
                     /gene_synonym="GluA3; GLUR-C; GLUR-K3; GLUR3; GLURC;
                     iGluR3; MRX94; MRXSW"
                     /inference="alignment:Splign:2.1.0"
     exon            767..954
                     /gene="GRIA3"
                     /gene_synonym="GluA3; GLUR-C; GLUR-K3; GLUR3; GLURC;
                     iGluR3; MRX94; MRXSW"
                     /inference="alignment:Splign:2.1.0"
     exon            955..1008
                     /gene="GRIA3"
                     /gene_synonym="GluA3; GLUR-C; GLUR-K3; GLUR3; GLURC;
                     iGluR3; MRX94; MRXSW"
                     /inference="alignment:Splign:2.1.0"
     exon            1009..1170
                     /gene="GRIA3"
                     /gene_synonym="GluA3; GLUR-C; GLUR-K3; GLUR3; GLURC;
                     iGluR3; MRX94; MRXSW"
                     /inference="alignment:Splign:2.1.0"
     exon            1171..1338
                     /gene="GRIA3"
                     /gene_synonym="GluA3; GLUR-C; GLUR-K3; GLUR3; GLURC;
                     iGluR3; MRX94; MRXSW"
                     /inference="alignment:Splign:2.1.0"
     exon            1339..1443
                     /gene="GRIA3"
                     /gene_synonym="GluA3; GLUR-C; GLUR-K3; GLUR3; GLURC;
                     iGluR3; MRX94; MRXSW"
                     /inference="alignment:Splign:2.1.0"
     exon            1444..1551
                     /gene="GRIA3"
                     /gene_synonym="GluA3; GLUR-C; GLUR-K3; GLUR3; GLURC;
                     iGluR3; MRX94; MRXSW"
                     /inference="alignment:Splign:2.1.0"
     exon            1552..1758
                     /gene="GRIA3"
                     /gene_synonym="GluA3; GLUR-C; GLUR-K3; GLUR3; GLURC;
                     iGluR3; MRX94; MRXSW"
                     /inference="alignment:Splign:2.1.0"
     exon            1759..2135
                     /gene="GRIA3"
                     /gene_synonym="GluA3; GLUR-C; GLUR-K3; GLUR3; GLURC;
                     iGluR3; MRX94; MRXSW"
                     /inference="alignment:Splign:2.1.0"
     exon            2136..2334
                     /gene="GRIA3"
                     /gene_synonym="GluA3; GLUR-C; GLUR-K3; GLUR3; GLURC;
                     iGluR3; MRX94; MRXSW"
                     /inference="alignment:Splign:2.1.0"
     exon            2335..2582
                     /gene="GRIA3"
                     /gene_synonym="GluA3; GLUR-C; GLUR-K3; GLUR3; GLURC;
                     iGluR3; MRX94; MRXSW"
                     /inference="alignment:Splign:2.1.0"
     exon            2583..2697
                     /gene="GRIA3"
                     /gene_synonym="GluA3; GLUR-C; GLUR-K3; GLUR3; GLURC;
                     iGluR3; MRX94; MRXSW"
                     /inference="alignment:Splign:2.1.0"
     exon            2698..2945
                     /gene="GRIA3"
                     /gene_synonym="GluA3; GLUR-C; GLUR-K3; GLUR3; GLURC;
                     iGluR3; MRX94; MRXSW"
                     /inference="alignment:Splign:2.1.0"
     exon            2946..5148
                     /gene="GRIA3"
                     /gene_synonym="GluA3; GLUR-C; GLUR-K3; GLUR3; GLURC;
                     iGluR3; MRX94; MRXSW"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5128..5133
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GRIA3"
                     /gene_synonym="GluA3; GLUR-C; GLUR-K3; GLUR3; GLURC;
                     iGluR3; MRX94; MRXSW"
                     /note="hexamer: AATAAA"
     polyA_site      5148
                     /gene="GRIA3"
                     /gene_synonym="GluA3; GLUR-C; GLUR-K3; GLUR3; GLURC;
                     iGluR3; MRX94; MRXSW"
                     /note="major polyA site"
ORIGIN      
        1 aagaaaggaa gagagagcga gcgagagaga gcgagcgaat aagagagaga gtaagaggga
       61 gagagaagaa gaggaagaag aggaggcggc ggcagcggag gaggaggagg actagtgtgg
      121 ggtggaaagg aagagtgagc gagagcaagt taaggggagg gggtgtaaga gccagcgaat
      181 tctttttctt tttctattat tattttgacg actcctgagt tgcgcccatg ctcttgtcag
      241 cttcgtttta ggcgtagcat ggccaggcag aagaaaatgg ggcaaagcgt gctccgggcg
      301 gtcttctttt tagtcctggg gcttttgggt cattctcacg gaggattccc caacaccatc
      361 agcataggtg gacttttcat gagaaacaca gtgcaggagc acagcgcttt ccgctttgcc
      421 gtgcagttat acaacaccaa ccagaacacc accgagaagc ccttccattt gaattaccac
      481 gtagatcact tggattcctc caatagtttt tccgtgacaa atgctttctg ctcccagttc
      541 tcgagagggg tgtatgccat ctttggattc tatgaccaga tgtcaatgaa caccctgacc
      601 tccttctgtg gggccctgca cacatccttt gttacgccta gcttccccac tgacgcagat
      661 gtgcagtttg tcatccagat gcgcccagcc ttgaagggcg ctattctgag tcttctgggt
      721 cattacaagt gggagaagtt tgtgtacctc tatgacacag aacgaggatt ttccatcctc
      781 caagcgatta tggaagcagc agtgcaaaac aactggcaag taacagcaag gtctgtggga
      841 aacataaagg acgtccaaga attcaggcgc atcattgaag aaatggacag gaggcaggaa
      901 aagcgatact tgattgactg cgaagtcgaa aggattaaca caattttgga acaggttgtg
      961 atcctaggga aacactcaag aggttatcac tacatgctcg ctaacctggg ttttactgat
     1021 attttactgg aaagagtcat gcatggggga gccaacatta caggtttcca gattgtcaac
     1081 aatgaaaacc ctatggttca gcagttcata cagcgctggg tgaggctgga tgaaagggaa
     1141 ttccctgaag ccaagaatgc accactaaag tatacatctg cattgacaca cgacgcaata
     1201 ctggtcatag cagaagcttt ccgctacctg aggaggcagc gagtagatgt gtcccggaga
     1261 ggaagtgctg gagactgctt agcaaatcct gctgtgccct ggagtcaagg aattgatatt
     1321 gagagagctc tgaaaatggt gcaagtacaa ggaatgactg gaaatattca atttgacact
     1381 tatggacgta ggacaaatta taccatcgat gtgtatgaaa tgaaagtcag tggctctcga
     1441 aaagctggct actggaatga gtatgaaagg tttgtgcctt tctcagatca gcaaatcagc
     1501 aatgacagtg catcctcaga gaatcggacc atagtagtga ctaccattct ggaatcacca
     1561 tatgtaatgt acaagaagaa ccatgagcaa ctggaaggaa atgaacgata tgaaggctat
     1621 tgtgtagacc tagcctatga aatagccaaa catgtaagga tcaaatacaa attgtccatc
     1681 gttggtgacg ggaaatatgg tgcaagggat ccagagacta aaatatggaa cggcatggtt
     1741 ggggaacttg tctatgggag agctgatata gctgttgctc cactcactat aacattggtc
     1801 cgtgaagaag tcatagattt ttcaaagcca ttcatgagcc tgggcatctc catcatgata
     1861 aagaagcctc agaaatcaaa accaggcgta ttctcatttc tggatcccct ggcttatgaa
     1921 atctggatgt gcattgtctt tgcttacatt ggagtcagcg tagttctttt cctagtcagc
     1981 aggttcagtc cttatgaatg gcacttggaa gacaacaatg aagaacctcg tgacccacaa
     2041 agtcctcctg atcctccaaa tgaatttgga atatttaaca gtctttggtt ttccttgggt
     2101 gcctttatgc agcaaggatg tgatatttct ccaagatcac tctccgggcg cattgttgga
     2161 ggggtttggt ggttcttcac cctgatcata atttcttcct atactgccaa tctcgctgct
     2221 ttcctgactg tggagaggat ggtttctccc atagagagtg ctgaagactt agctaaacag
     2281 actgaaattg catatgggac cctggactcc ggttcaacaa aagaattttt cagaagatcc
     2341 aaaattgctg tgtacgagaa aatgtggtct tacatgaaat cagcggagcc atctgtgttt
     2401 accaaaacaa cagcagacgg agtggcccga gtgcgaaagt ccaagggaaa gttcgccttc
     2461 ctgctggagt caaccatgaa tgagtacatt gagcagagaa aaccatgtga tacgatgaaa
     2521 gttggtggaa atctggattc caaaggctat ggtgtggcaa cccctaaagg ctcagcatta
     2581 agaacgcctg taaaccttgc agtattgaaa ctcagtgaac aaggcatctt agacaagctg
     2641 aaaaacaaat ggtggtacga taagggggaa tgtggagcca aggactccgg gagtaaggac
     2701 aagaccagcg ctctgagcct gagcaatgtg gcaggcgttt tctatatact tgtcggaggt
     2761 ctggggctgg ccatgatggt ggctttgata gaattctgtt acaaatcacg ggcagagtcc
     2821 aaacgcatga aactcacaaa gaacacccaa aactttaagc ctgctcctgc caccaacact
     2881 cagaattatg ctacatacag agaaggctac aacgtgtatg gaacagagag tgttaagatc
     2941 tagggatccc ttcccactgg aggcatgtga tgagaggaaa tcaccgaaaa cgtggctgct
     3001 tcaaggatcc tgagccagat ttcactctcc ttggtgtcgg gcatgacacg aatattgctg
     3061 atggtgcaat gacctttcaa taggaaaaac tgattttttt ttccttcagt gccttatgga
     3121 acactctgag actcgcgaca atgcaaacca tcattgaaat ctttttgctt tgcttgaaaa
     3181 aaaataatta aaataaaaac caacaaaaat ggacatgcaa gattccagta tgcgaaaaaa
     3241 aatcttatta agtcaattca acaaaagcca ttctttgata ccactgcaga gtatataaac
     3301 accatgttct ttaatacaca cacacacaca cacacacaca cacacacaca tttaaattcc
     3361 aattcagcaa agaggcccat ctaagctaaa aaaattaatt cttcctgatt aaaaagaaaa
     3421 aatctgtctc ccagtgtttg ggaagacgga ctggcatttc ttctaggatc tgctgaccag
     3481 atgtttttgg tatttcctgt tggtggtgat gttctgtgca ctctatttcc tttcaatgtt
     3541 gctgaaatgt gtatatcttt agaatgtaaa tgcaacactt aagaaaattc aaacactttg
     3601 gaaaagggac taaacagtga tttctctgtg ttcttgaaat ggttttgtga aaatgctttg
     3661 ataacttccc actcaaagaa gagatttaca gagctttcga aattgacttt gtgtgtagca
     3721 agggacgggg cactatcagg atacctcttg gtgctttcct aaaatggatc ccggggcttt
     3781 ccaaggagcc tggaatttca gctcacagat ctgtttttct tgcttcagtg tgcattttaa
     3841 gtcaatagag ctgagtatct agcattgagg tgagggaaat gctgcctata ctcccagatg
     3901 tgtttagaat atctcagaaa caacactgtg tttagctcgg ctttctctgc taagtatgcc
     3961 tttcaagtgt acaccacgga gacaggaccg cgttgcaagg cgggacagca ggttcagacc
     4021 acagttctca gtctgacttt actcttgcta ggtctgtcct actagctgtt gcctgctacc
     4081 gcccatggct ctccatcgga ctgcatgtgt ccttttctag tttgcaaaga ctaaaatgca
     4141 ttcccaaacc tactgctaat ctgagggcct cagcatcact tccagatcct tgcttggagc
     4201 agtctctcta ttgactctct cagatcgctc cactgctcca tgggctatca agtaactaac
     4261 tgcatacctg ccgttggcat catcagaaca gtccgaagaa atagtctcca ctcactaatt
     4321 acctcctata taacgacgta tgcttcctgt agttcagtag tttgctctca tcgataacgt
     4381 gcattgggaa gtttccagac tgcaaaaact aggagctcgc attcatttcc caagtgtgac
     4441 ccttagatgc ttagttgact cgctgcatat ttgctcttgt cttcagaaaa gaaaggaaga
     4501 agtatcgttc caacgaaatg tttccagaaa agtgtactat aaactttcat tccaaaaatg
     4561 gtgtcataag caaacaactc acttgtcaaa tttcaaatgg tattgaacaa aaaaagaaag
     4621 ctgttgtgtt tttgttttgt tttgttttca tgaaactgtg attttcaact tatgaatgct
     4681 ataatgtccc agcgcgggaa gctcacgctg tgtgaacatg aagttgtata aaacaaacca
     4741 accaacctac acacaaatgt tttcataggc actgtataaa gaaaaatgta tgtttattaa
     4801 ctcaaatcag tttttcagag aggaaacgtc actgagatga agaggcgggt aaattggttt
     4861 gttatttttt aaaaaaaact tgcatgttta aaaaaaagtt gattgcttca aatttctgct
     4921 actaacttca agctatggga gtttggcagt agtcacttga ggattttttt tccaattctt
     4981 ttctttttgt tgttaaagct gtacttcagt gaacagaaaa attgccaagc aaactaatgg
     5041 ctataaaagc gtaatttgca tgtgtgggca taaactacag agcctcattg ccatgaggta
     5101 ttgtacaaag ttttaataca ttttgtaaat aaaattgtaa agaaagaa
//
