LOCUS       NM_001083619            5611 bp    mRNA    linear   PRI 03-OCT-2024
DEFINITION  Homo sapiens glutamate ionotropic receptor AMPA type subunit 2
            (GRIA2), transcript variant 2, mRNA.
ACCESSION   NM_001083619
VERSION     NM_001083619.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5611)
  AUTHORS   Coombs,I.D., Ziobro,J., Krotov,V., Surtees,T.L., Cull-Candy,S.G.
            and Farrant,M.
  TITLE     A gain-of-function GRIA2 variant associated with neurodevelopmental
            delay and seizures: Functional characterization and targeted
            treatment
  JOURNAL   Epilepsia 63 (12), e156-e163 (2022)
   PUBMED   36161652
REFERENCE   2  (bases 1 to 5611)
  AUTHORS   La,T.M., Yamada,H., Seiriki,S., Li,S.A., Fujise,K., Katsumi,N.,
            Abe,T., Watanabe,M. and Takei,K.
  TITLE     Internalization of AMPA-type Glutamate Receptor in the MIN6
            Pancreatic beta-cell Line
  JOURNAL   Cell Struct Funct 45 (2), 121-130 (2020)
   PUBMED   32581155
  REMARK    GeneRIF: Internalization of AMPA-type Glutamate Receptor in the
            MIN6 Pancreatic beta-cell Line.
REFERENCE   3  (bases 1 to 5611)
  AUTHORS   Qneibi,M., Hamed,O., Natsheh,A.R., Fares,O., Jaradat,N., Emwas,N.,
            AbuHasan,Q., Al-Kerm,R. and Al-Kerm,R.
  TITLE     Inhibition and assessment of the biophysical gating properties of
            GluA2 and GluA2/A3 AMPA receptors using curcumin derivatives
  JOURNAL   PLoS One 14 (8), e0221132 (2019)
   PUBMED   31454362
  REMARK    GeneRIF: he current study investigated novel curcumin derivatives
            on the biophysical properties of AMPA receptors, specifically on
            the homomeric GluA2 and the heteromeric GluA2/A3 subunits and
            assessed for inhibitory actions
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5611)
  AUTHORS   Salpietro,V., Dixon,C.L., Guo,H., Bello,O.D., Vandrovcova,J.,
            Efthymiou,S., Maroofian,R., Heimer,G., Burglen,L., Valence,S.,
            Torti,E., Hacke,M., Rankin,J., Tariq,H., Colin,E., Procaccio,V.,
            Striano,P., Mankad,K., Lieb,A., Chen,S., Pisani,L., Bettencourt,C.,
            Mannikko,R., Manole,A., Brusco,A., Grosso,E., Ferrero,G.B.,
            Armstrong-Moron,J., Gueden,S., Bar-Yosef,O., Tzadok,M.,
            Monaghan,K.G., Santiago-Sim,T., Person,R.E., Cho,M.T., Willaert,R.,
            Yoo,Y., Chae,J.H., Quan,Y., Wu,H., Wang,T., Bernier,R.A., Xia,K.,
            Blesson,A., Jain,M., Motazacker,M.M., Jaeger,B., Schneider,A.L.,
            Boysen,K., Muir,A.M., Myers,C.T., Gavrilova,R.H., Gunderson,L.,
            Schultz-Rogers,L., Klee,E.W., Dyment,D., Osmond,M., Parellada,M.,
            Llorente,C., Gonzalez-Penas,J., Carracedo,A., Van Haeringen,A.,
            Ruivenkamp,C., Nava,C., Heron,D., Nardello,R., Iacomino,M.,
            Minetti,C., Skabar,A., Fabretto,A., Raspall-Chaure,M., Chez,M.,
            Tsai,A., Fassi,E., Shinawi,M., Constantino,J.N., De Zorzi,R.,
            Fortuna,S., Kok,F., Keren,B., Bonneau,D., Choi,M., Benzeev,B.,
            Zara,F., Mefford,H.C., Scheffer,I.E., Clayton-Smith,J., Macaya,A.,
            Rothman,J.E., Eichler,E.E., Kullmann,D.M. and Houlden,H.
  CONSRTM   SYNAPS Study Group
  TITLE     AMPA receptor GluA2 subunit defects are a cause of
            neurodevelopmental disorders
  JOURNAL   Nat Commun 10 (1), 3094 (2019)
   PUBMED   31300657
  REMARK    GeneRIF: Study reports heterozygous de novo GRIA2 mutations in 28
            unrelated patients with intellectual disability and
            neurodevelopmental abnormalities. When GluA2 subunits are
            co-expressed with GluA1, most GRIA2 mutations cause a decreased
            current amplitude and some also affect voltage rectification.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 5611)
  AUTHORS   Higuchi,M., Single,F.N., Kohler,M., Sommer,B., Sprengel,R. and
            Seeburg,P.H.
  TITLE     RNA editing of AMPA receptor subunit GluR-B: a base-paired
            intron-exon structure determines position and efficiency
  JOURNAL   Cell 75 (7), 1361-1370 (1993)
   PUBMED   8269514
REFERENCE   6  (bases 1 to 5611)
  AUTHORS   Efthymiou,S., Rumbos Siurana,E., Salpietro,V., Bayat,A. and
            Houlden,H.
  TITLE     GRIA2-Related Neurodevelopmental Disorder
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   38224558
REFERENCE   7  (bases 1 to 5611)
  AUTHORS   McNamara,J.O., Eubanks,J.H., McPherson,J.D., Wasmuth,J.J.,
            Evans,G.A. and Heinemann,S.F.
  TITLE     Chromosomal localization of human glutamate receptor genes
  JOURNAL   J Neurosci 12 (7), 2555-2562 (1992)
   PUBMED   1319477
REFERENCE   8  (bases 1 to 5611)
  AUTHORS   Sun,W., Ferrer-Montiel,A.V., Schinder,A.F., McPherson,J.P.,
            Evans,G.A. and Montal,M.
  TITLE     Molecular cloning, chromosomal mapping, and functional expression
            of human brain glutamate receptors
  JOURNAL   Proc Natl Acad Sci U S A 89 (4), 1443-1447 (1992)
   PUBMED   1311100
REFERENCE   9  (bases 1 to 5611)
  AUTHORS   Sommer,B., Kohler,M., Sprengel,R. and Seeburg,P.H.
  TITLE     RNA editing in brain controls a determinant of ion flow in
            glutamate-gated channels
  JOURNAL   Cell 67 (1), 11-19 (1991)
   PUBMED   1717158
REFERENCE   10 (bases 1 to 5611)
  AUTHORS   Sommer,B., Keinanen,K., Verdoorn,T.A., Wisden,W., Burnashev,N.,
            Herb,A., Kohler,M., Takagi,T., Sakmann,B. and Seeburg,P.H.
  TITLE     Flip and flop: a cell-specific functional switch in
            glutamate-operated channels of the CNS
  JOURNAL   Science 249 (4976), 1580-1585 (1990)
   PUBMED   1699275
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC112240.4 and AC079233.4.
            
            On Mar 28, 2020 this sequence version replaced NM_001083619.2.
            
            Summary: Glutamate receptors are the predominant excitatory
            neurotransmitter receptors in the mammalian brain and are activated
            in a variety of normal neurophysiologic processes. This gene
            product belongs to a family of glutamate receptors that are
            sensitive to alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate
            (AMPA), and function as ligand-activated cation channels. These
            channels are assembled from 4 related subunits, GRIA1-4. The
            subunit encoded by this gene (GRIA2) is subject to RNA editing
            (CAG->CGG; Q->R) within the second transmembrane domain, which is
            thought to render the channel impermeable to Ca(2+). Human and
            animal studies suggest that pre-mRNA editing is essential for brain
            function, and defective GRIA2 RNA editing at the Q/R site may be
            relevant to amyotrophic lateral sclerosis (ALS) etiology.
            Alternative splicing, resulting in transcript variants encoding
            different isoforms, (including the flip and flop isoforms that vary
            in their signal transduction properties), has been noted for this
            gene. [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC010574.1, AK309067.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2145544, SAMEA2145743
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000264426.14/ ENSP00000264426.9
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      longest protein
            undergoes RNA editing  :: PMID: 8269514, 15006707, 14985749
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-403               AC112240.4         22583-22985
            404-544             AC112240.4         23522-23662
            545-784             AC079233.4         91127-91366         c
            785-981             AC079233.4         82043-82239         c
            982-1035            AC079233.4         77207-77260         c
            1036-1197           AC079233.4         73319-73480         c
            1198-1365           AC079233.4         61932-62099         c
            1366-1470           AC079233.4         61565-61669         c
            1471-1581           AC079233.4         60798-60908         c
            1582-1788           AC079233.4         59041-59247         c
            1789-2159           AC079233.4         58171-58541         c
            2160-2358           AC079233.4         53456-53654         c
            2359-2606           AC079233.4         34775-35022         c
            2607-2721           AC079233.4         33794-33908         c
            2722-2970           AC079233.4         31871-32119         c
            2971-5611           AC079233.4         28843-31483         c
FEATURES             Location/Qualifiers
     source          1..5611
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="4"
                     /map="4q32.1"
     gene            1..5611
                     /gene="GRIA2"
                     /gene_synonym="GluA2; gluR-2; gluR-B; GluR-K2; GLUR2;
                     GLURB; HBGR2; NEDLIB"
                     /note="glutamate ionotropic receptor AMPA type subunit 2"
                     /db_xref="GeneID:2891"
                     /db_xref="HGNC:HGNC:4572"
                     /db_xref="MIM:138247"
     exon            1..403
                     /gene="GRIA2"
                     /gene_synonym="GluA2; gluR-2; gluR-B; GluR-K2; GLUR2;
                     GLURB; HBGR2; NEDLIB"
                     /inference="alignment:Splign:2.1.0"
     CDS             316..2967
                     /gene="GRIA2"
                     /gene_synonym="GluA2; gluR-2; gluR-B; GluR-K2; GLUR2;
                     GLURB; HBGR2; NEDLIB"
                     /note="isoform 2 precursor is encoded by transcript
                     variant 2; glutamate receptor 2; AMPA-selective glutamate
                     receptor 2; AMPA receptor subunit GluA2; glutamate
                     receptor, ionotropic, AMPA 2"
                     /codon_start=1
                     /product="glutamate receptor 2 isoform 2 precursor"
                     /protein_id="NP_001077088.2"
                     /db_xref="CCDS:CCDS43274.1"
                     /db_xref="GeneID:2891"
                     /db_xref="HGNC:HGNC:4572"
                     /db_xref="MIM:138247"
                     /translation="MQKIMHISVLLSPVLWGLIFGVSSNSIQIGGLFPRGADQEYSAF
                     RVGMVQFSTSEFRLTPHIDNLEVANSFAVTNAFCSQFSRGVYAIFGFYDKKSVNTITS
                     FCGTLHVSFITPSFPTDGTHPFVIQMRPDLKGALLSLIEYYQWDKFAYLYDSDRGLST
                     LQAVLDSAAEKKWQVTAINVGNINNDKKDEMYRSLFQDLELKKERRVILDCERDKVND
                     IVDQVITIGKHVKGYHYIIANLGFTDGDLLKIQFGGANVSGFQIVDYDDSLVSKFIER
                     WSTLEEKEYPGAHTTTIKYTSALTYDAVQVMTEAFRNLRKQRIEISRRGNAGDCLANP
                     AVPWGQGVEIERALKQVQVEGLSGNIKFDQNGKRINYTINIMELKTNGPRKIGYWSEV
                     DKMVVTLTELPSGNDTSGLENKTVVVTTILESPYVMMKKNHEMLEGNERYEGYCVDLA
                     AEIAKHCGFKYKLTIVGDGKYGARDADTKIWNGMVGELVYGKADIAIAPLTITLVREE
                     VIDFSKPFMSLGISIMIKKPQKSKPGVFSFLDPLAYEIWMCIVFAYIGVSVVLFLVSR
                     FSPYEWHTEEFEDGRETQSSESTNEFGIFNSLWFSLGAFMQQGCDISPRSLSGRIVGG
                     VWWFFTLIIISSYTANLAAFLTVERMVSPIESAEDLSKQTEIAYGTLDSGSTKEFFRR
                     SKIAVFDKMWTYMRSAEPSVFVRTTAEGVARVRKSKGKYAYLLESTMNEYIEQRKPCD
                     TMKVGGNLDSKGYGIATPKGSSLRNAVNLAVLKLNEQGLLDKLKNKWWYDKGECGSGG
                     GDSKEKTSALSLSNVAGVFYILVGGLGLAMLVALIEFCYKSRAEAKRMKVAKNAQNIN
                     PSSSQNSQNFATYKEGYNVYGIESVKI"
     exon            404..544
                     /gene="GRIA2"
                     /gene_synonym="GluA2; gluR-2; gluR-B; GluR-K2; GLUR2;
                     GLURB; HBGR2; NEDLIB"
                     /inference="alignment:Splign:2.1.0"
     exon            545..784
                     /gene="GRIA2"
                     /gene_synonym="GluA2; gluR-2; gluR-B; GluR-K2; GLUR2;
                     GLURB; HBGR2; NEDLIB"
                     /inference="alignment:Splign:2.1.0"
     exon            785..981
                     /gene="GRIA2"
                     /gene_synonym="GluA2; gluR-2; gluR-B; GluR-K2; GLUR2;
                     GLURB; HBGR2; NEDLIB"
                     /inference="alignment:Splign:2.1.0"
     exon            982..1035
                     /gene="GRIA2"
                     /gene_synonym="GluA2; gluR-2; gluR-B; GluR-K2; GLUR2;
                     GLURB; HBGR2; NEDLIB"
                     /inference="alignment:Splign:2.1.0"
     exon            1036..1197
                     /gene="GRIA2"
                     /gene_synonym="GluA2; gluR-2; gluR-B; GluR-K2; GLUR2;
                     GLURB; HBGR2; NEDLIB"
                     /inference="alignment:Splign:2.1.0"
     exon            1198..1365
                     /gene="GRIA2"
                     /gene_synonym="GluA2; gluR-2; gluR-B; GluR-K2; GLUR2;
                     GLURB; HBGR2; NEDLIB"
                     /inference="alignment:Splign:2.1.0"
     exon            1366..1470
                     /gene="GRIA2"
                     /gene_synonym="GluA2; gluR-2; gluR-B; GluR-K2; GLUR2;
                     GLURB; HBGR2; NEDLIB"
                     /inference="alignment:Splign:2.1.0"
     exon            1471..1581
                     /gene="GRIA2"
                     /gene_synonym="GluA2; gluR-2; gluR-B; GluR-K2; GLUR2;
                     GLURB; HBGR2; NEDLIB"
                     /inference="alignment:Splign:2.1.0"
     exon            1582..1788
                     /gene="GRIA2"
                     /gene_synonym="GluA2; gluR-2; gluR-B; GluR-K2; GLUR2;
                     GLURB; HBGR2; NEDLIB"
                     /inference="alignment:Splign:2.1.0"
     exon            1789..2159
                     /gene="GRIA2"
                     /gene_synonym="GluA2; gluR-2; gluR-B; GluR-K2; GLUR2;
                     GLURB; HBGR2; NEDLIB"
                     /inference="alignment:Splign:2.1.0"
     exon            2160..2358
                     /gene="GRIA2"
                     /gene_synonym="GluA2; gluR-2; gluR-B; GluR-K2; GLUR2;
                     GLURB; HBGR2; NEDLIB"
                     /inference="alignment:Splign:2.1.0"
     exon            2359..2606
                     /gene="GRIA2"
                     /gene_synonym="GluA2; gluR-2; gluR-B; GluR-K2; GLUR2;
                     GLURB; HBGR2; NEDLIB"
                     /inference="alignment:Splign:2.1.0"
     exon            2607..2721
                     /gene="GRIA2"
                     /gene_synonym="GluA2; gluR-2; gluR-B; GluR-K2; GLUR2;
                     GLURB; HBGR2; NEDLIB"
                     /inference="alignment:Splign:2.1.0"
     exon            2722..2970
                     /gene="GRIA2"
                     /gene_synonym="GluA2; gluR-2; gluR-B; GluR-K2; GLUR2;
                     GLURB; HBGR2; NEDLIB"
                     /inference="alignment:Splign:2.1.0"
     exon            2971..5611
                     /gene="GRIA2"
                     /gene_synonym="GluA2; gluR-2; gluR-B; GluR-K2; GLUR2;
                     GLURB; HBGR2; NEDLIB"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5584..5589
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GRIA2"
                     /gene_synonym="GluA2; gluR-2; gluR-B; GluR-K2; GLUR2;
                     GLURB; HBGR2; NEDLIB"
                     /note="hexamer: CATAAA"
     polyA_site      5611
                     /gene="GRIA2"
                     /gene_synonym="GluA2; gluR-2; gluR-B; GluR-K2; GLUR2;
                     GLURB; HBGR2; NEDLIB"
                     /note="major polyA site"
ORIGIN      
        1 gagtgtgtgt gagtgcatgg gagggtgctg aatattccga gacactggga ccacagcggc
       61 agctccgctg aaaactgcat tcagccagtc ctccggactt ctggagcggg gacagggcgc
      121 agggcatcag cagccaccag caggacctgg gaaataggga ttcttctgcc tccacttcag
      181 gttttagcag cttggtgcta aattgctgtc tcaaaatgca gaggatctaa tttgcagagg
      241 aaaacagcca aagaaggaag aggaggaaaa ggaaaaaaaa aggggtatat tgtggatgct
      301 ctacttttct tggaaatgca aaagattatg catatttctg tcctcctttc tcctgtttta
      361 tggggactga tttttggtgt ctcttctaac agcatacaga taggggggct atttcctagg
      421 ggcgccgatc aagaatacag tgcatttcga gtagggatgg ttcagttttc cacttcggag
      481 ttcagactga caccccacat cgacaatttg gaggtggcaa acagcttcgc agtcactaat
      541 gctttctgct cccagttttc gagaggagtc tatgctattt ttggatttta tgacaagaag
      601 tctgtaaata ccatcacatc attttgcgga acactccacg tctccttcat cactcccagc
      661 ttcccaacag atggcacaca tccatttgtc attcagatga gacccgacct caaaggagct
      721 ctccttagct tgattgaata ctatcaatgg gacaagtttg catacctcta tgacagtgac
      781 agaggcttat caacactgca agctgtgctg gattctgctg ctgaaaagaa atggcaagtg
      841 actgctatca atgtgggaaa cattaacaat gacaagaaag atgagatgta ccgatcactt
      901 tttcaagatc tggagttaaa aaaggaacgg cgtgtaattc tggactgtga aagggataaa
      961 gtaaacgaca ttgtagacca ggttattacc attggaaaac atgttaaagg gtaccactac
     1021 atcattgcaa atctgggatt tactgatgga gacctattaa aaatccagtt tggaggtgca
     1081 aatgtctctg gatttcagat agtggactat gatgattcgt tggtatctaa atttatagaa
     1141 agatggtcaa cactggaaga aaaagaatac cctggagctc acacaacaac aattaagtat
     1201 acttctgctc tgacctatga tgccgttcaa gtgatgactg aagccttccg caacctaagg
     1261 aagcaaagaa ttgaaatctc ccgaaggggg aatgcaggag actgtctggc aaacccagca
     1321 gtgccctggg gacaaggtgt agaaatagaa agggccctca aacaggttca ggttgaaggt
     1381 ctctcaggaa atataaagtt tgaccagaat ggaaaaagaa taaactatac aattaacatc
     1441 atggagctca aaactaatgg gccccggaag attggctact ggagtgaagt ggacaaaatg
     1501 gttgttaccc ttactgagct cccttctgga aatgacacct ctgggcttga gaataagact
     1561 gttgttgtca ccacaatttt ggaatctccg tatgttatga tgaagaaaaa tcatgaaatg
     1621 cttgaaggca atgagcgcta tgagggctac tgtgttgacc tggctgcaga aatcgccaaa
     1681 cattgtgggt tcaagtacaa gttgacaatt gttggtgatg gcaagtatgg ggccagggat
     1741 gcagacacga aaatttggaa tgggatggtt ggagaacttg tatatgggaa agctgatatt
     1801 gcaattgctc cattaactat tacccttgtg agagaagagg tgattgactt ctcaaagccc
     1861 ttcatgagcc tcgggatatc tatcatgatc aagaagcctc agaagtccaa accaggagtg
     1921 ttttcctttc ttgatccttt agcctatgag atctggatgt gcattgtttt tgcctacatt
     1981 ggggtcagtg tagttttatt cctggtcagc agatttagcc cctacgagtg gcacactgag
     2041 gagtttgaag atggaagaga aacacaaagt agtgaatcaa ctaatgaatt tgggattttt
     2101 aatagtctct ggttttcctt gggtgccttt atgcagcaag gatgcgatat ttcgccaaga
     2161 tccctctctg ggcgcattgt tggaggtgtg tggtggttct ttaccctgat cataatctcc
     2221 tcctacacgg ctaacttagc tgccttcctg actgtagaga ggatggtgtc tcccatcgaa
     2281 agtgctgagg atctttctaa gcaaacagaa attgcttatg gaacattaga ctctggctcc
     2341 actaaagagt ttttcaggag atctaaaatt gcagtgtttg ataaaatgtg gacctacatg
     2401 cggagtgcgg agccctctgt gtttgtgagg actacggccg aaggggtggc tagagtgcgg
     2461 aagtccaaag ggaaatatgc ctacttgttg gagtccacga tgaacgagta cattgagcaa
     2521 aggaagcctt gcgacaccat gaaagttggt ggaaacctgg attccaaagg ctatggcatc
     2581 gcaacaccta aaggatcctc attaagaaat gcggttaacc tcgcagtact aaaactgaat
     2641 gaacaaggcc tgttggacaa attgaaaaac aaatggtggt acgacaaagg agagtgcggc
     2701 agcgggggag gtgattccaa ggaaaagacc agtgccctca gtctgagcaa cgttgctgga
     2761 gtattctaca tccttgtcgg gggccttggt ttggcaatgc tggtggcttt gattgagttc
     2821 tgttacaagt caagggccga ggcgaaacga atgaaggtgg caaagaatgc acagaatatt
     2881 aacccatctt cctcgcagaa ttcacagaat tttgcaactt ataaggaagg ttacaacgta
     2941 tatggcatcg aaagtgttaa aatttagggg atgaccttga atgatgccat gaggaacaag
     3001 gcaaggctgt caattacagg aagtactgga gaaaatggac gtgttatgac tccagaattt
     3061 cccaaagcag tgcatgctgt cccttacgtg agtcctggca tgggaatgaa tgtcagtgtg
     3121 actgatctct cgtgattgat aagaaccttt tgagtgcctt acacaatggt tttcttgtgt
     3181 gtttattgtc aaagtggtga gaggcatcca gtatcttgaa gacttttctt tcagccaaga
     3241 attcttaaat atgtggagtt catcttgaat tgtaaggaat gattaattaa aacacaacat
     3301 ctttttctac tcgagttaca gacaaagcgt ggtggacatg cacagctaac atggaagtac
     3361 tataatttac ctgaagtctt tgtacagaca acaaacctgt ttctgcagcc actattgtta
     3421 gtctcttgat tcataatgac ttaagcacac ttgacatcaa ctgcatcaag atgtgacatg
     3481 ttttataaaa aaaggaaaaa aaacatttaa aactaaaaaa tatttttagg tattttcaca
     3541 aacaaactgg cttttaaata aatttgcttc catattggtt gaataagaca aaaacaatta
     3601 aactgagtgg gaagtgaata aaaaaaggct ttaggtatcg attccatatt tttcaaagcc
     3661 aaatatgtaa atgctaagga aagtaaacaa agaggagatt ccaatcttgt aatttaatat
     3721 tgttattaaa actttaatgt atcctattct ttaacatttg gtgttaatat aaaattactt
     3781 ggcaatgctt gacatttgaa ataaacattt ttctattgtt ttattgcaag tggtccaatt
     3841 aattttgctt agctacagtt tggtcataaa tcaagtgagt ttaaagacac taccaagttg
     3901 ttaggtgccc agagaaaatt tctccctttt aaaaaggcca ggtgattttt caaatgtaat
     3961 cttgccccca aagtaatatc tgaatatctt tttgacatgt ctaaatatat atatatataa
     4021 agaaatattt gttaacacaa aagcatttga tctatgtaga taaatgctaa tagatttaaa
     4081 aagctaatat taacaaatac cagaatacgt gaagttccat ttttaaagtg tttgagctta
     4141 cagaagagaa acattcattt taaatgaagt aaaaaatgcc ttgaaagtaa ttctttagat
     4201 agttgcccat tgattaaatt ccaaaaacta aatatgtttt tagctttaaa attataaaag
     4261 ctgtcataaa ctttatatat tatgaatttt aaaatatgtt tgagtctcct gcaatatagt
     4321 ttcatcccat tgacatcaat taaaaataac cctaatatat tatttttata tttattcctc
     4381 aggtggaatg gctattttaa tatgcccagt gtggataaaa tgtcacattt ctgtaacttt
     4441 tgactaaaga gcctatattt atctagttaa tgaatttaaa ggatctatct ttcccttcat
     4501 aaaatacctc ttatttccat taaagccccc caagtttaat taatttagga ttttgaatga
     4561 ttattgacat ccaatagtta tttttaatat ttgtattctt gttatttctg gaagaaagcc
     4621 tttgtgtagc acttggtatt ttgcaaagtg cttttaaaac attcttactt accgtatttc
     4681 atagaaggga aggaaaaatg taaggtttaa cagtaagcac ttgcattgaa catggaggca
     4741 tgtggtatca tgatattctt cactaaattt agctgtccct aatcacagat cctaaggtaa
     4801 tataatataa ttttagtgca tttctcctca tcaggaatgc tggaggtgca ttttaagttt
     4861 taataataag tgctagaatg accaaattgc agactaattg tttccatatt gtacttaaaa
     4921 tgagttttta aaagtgaaaa agaaatgact atatacaatc aatgctattt attgtacctc
     4981 tgggcctact cttctaaaaa ttgtagctta tcgatttttc tctgtcaagc ttgaactaat
     5041 gtaaataatt gaaataatgt aaagttatat tttcatgttt ttatagatac aacatgacaa
     5101 gaatacataa tgtaagagta tttcaactat ggataatgtt gattggataa tgcacatctc
     5161 agttacaagc agtactcata gtttaatatc catgtaacgg tgcatcaata tattgctata
     5221 taaatatgtc tgtgtgcata taagtgaaaa gtggtcaaac aagagtgatg acagctgtct
     5281 aaaggttttt ttattcattt tatataaaaa ctgttatgga aagaccaaaa tgtttatgaa
     5341 ctattcttat gtaaatttac aattgtcctt tactgtactt ttttgtttac agtatagtac
     5401 cttattttct gctgtgttaa gtgggtgtca aactccaaga agacatacac tttctataac
     5461 ttctattgaa gatattggaa tttccaattt ttcatgtgta ctatgtcaga aaatgctttc
     5521 gattttattt ttaaatctaa catcggatgg cttttccgga gtgttgtaaa aacttcaatc
     5581 atacataaaa catgttctta caaaaggcaa a
//
