LOCUS       NM_000827               5584 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens glutamate ionotropic receptor AMPA type subunit 1
            (GRIA1), transcript variant 1, mRNA.
ACCESSION   NM_000827
VERSION     NM_000827.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5584)
  AUTHORS   Lai,S.K., Wu,K.L.K., Ma,C.W., Ng,K.P., Hu,X.Q., Tam,K.W.,
            Yung,W.H., Wang,Y.T., Wong,T.P., Shum,D.K. and Chan,Y.S.
  TITLE     Timely insertion of AMPA receptor in developing vestibular circuits
            is required for manifestation of righting reflexes and effective
            navigation
  JOURNAL   Prog Neurobiol 221, 102402 (2023)
   PUBMED   36608782
  REMARK    GeneRIF: Timely insertion of AMPA receptor in developing vestibular
            circuits is required for manifestation of righting reflexes and
            effective navigation.
REFERENCE   2  (bases 1 to 5584)
  AUTHORS   Ismail,V., Zachariassen,L.G., Godwin,A., Sahakian,M., Ellard,S.,
            Stals,K.L., Baple,E., Brown,K.T., Foulds,N., Wheway,G.,
            Parker,M.O., Lyngby,S.M., Pedersen,M.G., Desir,J., Bayat,A.,
            Musgaard,M., Guille,M., Kristensen,A.S. and Baralle,D.
  TITLE     Identification and functional evaluation of GRIA1 missense and
            truncation variants in individuals with ID: An emerging
            neurodevelopmental syndrome
  JOURNAL   Am J Hum Genet 109 (7), 1217-1241 (2022)
   PUBMED   35675825
  REMARK    GeneRIF: Identification and functional evaluation of GRIA1 missense
            and truncation variants in individuals with ID: An emerging
            neurodevelopmental syndrome.
REFERENCE   3  (bases 1 to 5584)
  AUTHORS   Zhao,M., Dong,J., Liao,Y., Lu,G., Pan,W., Zhou,H., Zuo,X. and
            Shan,B.
  TITLE     MicroRNA miR-18a-3p promotes osteoporosis and possibly contributes
            to spinal fracture by inhibiting the glutamate AMPA receptor
            subunit 1 gene (GRIA1)
  JOURNAL   Bioengineered 13 (1), 370-382 (2022)
   PUBMED   34937502
  REMARK    GeneRIF: MicroRNA miR-18a-3p promotes osteoporosis and possibly
            contributes to spinal fracture by inhibiting the glutamate AMPA
            receptor subunit 1 gene (GRIA1).
REFERENCE   4  (bases 1 to 5584)
  AUTHORS   Ge,Y. and Wang,Y.T.
  TITLE     GluA1-homomeric AMPA receptor in synaptic plasticity and
            neurological diseases
  JOURNAL   Neuropharmacology 197, 108708 (2021)
   PUBMED   34274350
  REMARK    GeneRIF: GluA1-homomeric AMPA receptor in synaptic plasticity and
            neurological diseases.
            Review article
REFERENCE   5  (bases 1 to 5584)
  AUTHORS   Melo,H.M., de Carvalho,C.R., Hoeller,A.A., Marques,J.L.B.,
            Linhares,M.N., Lopes,M.W., Fialho,G.L., Wolf,P., Lin,K.,
            Bortolotto,Z.A., Henley,J.M., D'Avila,A., Leal,R.B. and Walz,R.
  TITLE     AMPAr GluA1 Phosphorylation at Serine 845 in Limbic System Is
            Associated with Cardiac Autonomic Tone
  JOURNAL   Mol Neurobiol 58 (4), 1859-1870 (2021)
   PUBMED   33404979
  REMARK    GeneRIF: AMPAr GluA1 Phosphorylation at Serine 845 in Limbic System
            Is Associated with Cardiac Autonomic Tone.
REFERENCE   6  (bases 1 to 5584)
  AUTHORS   Yakel,J.L., Vissavajjhala,P., Derkach,V.A., Brickey,D.A. and
            Soderling,T.R.
  TITLE     Identification of a Ca2+/calmodulin-dependent protein kinase II
            regulatory phosphorylation site in non-N-methyl-D-aspartate
            glutamate receptors
  JOURNAL   Proc Natl Acad Sci U S A 92 (5), 1376-1380 (1995)
   PUBMED   7877986
REFERENCE   7  (bases 1 to 5584)
  AUTHORS   McNamara,J.O., Eubanks,J.H., McPherson,J.D., Wasmuth,J.J.,
            Evans,G.A. and Heinemann,S.F.
  TITLE     Chromosomal localization of human glutamate receptor genes
  JOURNAL   J Neurosci 12 (7), 2555-2562 (1992)
   PUBMED   1319477
REFERENCE   8  (bases 1 to 5584)
  AUTHORS   Sun,W., Ferrer-Montiel,A.V., Schinder,A.F., McPherson,J.P.,
            Evans,G.A. and Montal,M.
  TITLE     Molecular cloning, chromosomal mapping, and functional expression
            of human brain glutamate receptors
  JOURNAL   Proc Natl Acad Sci U S A 89 (4), 1443-1447 (1992)
   PUBMED   1311100
REFERENCE   9  (bases 1 to 5584)
  AUTHORS   Potier,M.C., Spillantini,M.G. and Carter,N.P.
  TITLE     The human glutamate receptor cDNA GluR1: cloning, sequencing,
            expression and localization to chromosome 5
  JOURNAL   DNA Seq 2 (4), 211-218 (1992)
   PUBMED   1320959
REFERENCE   10 (bases 1 to 5584)
  AUTHORS   Puckett,C., Gomez,C.M., Korenberg,J.R., Tung,H., Meier,T.J.,
            Chen,X.N. and Hood,L.
  TITLE     Molecular cloning and chromosomal localization of one of the human
            glutamate receptor genes
  JOURNAL   Proc Natl Acad Sci U S A 88 (17), 7557-7561 (1991)
   PUBMED   1652753
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DC335107.1, AK310189.1,
            M81886.1, X58633.1, AB209094.1 and AL120214.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_000827.3.
            
            Summary: Glutamate receptors are the predominant excitatory
            neurotransmitter receptors in the mammalian brain and are activated
            in a variety of normal neurophysiologic processes. These receptors
            are heteromeric protein complexes with multiple subunits, each
            possessing transmembrane regions, and all arranged to form a
            ligand-gated ion channel. The classification of glutamate receptors
            is based on their activation by different pharmacologic agonists.
            This gene belongs to a family of
            alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA)
            receptors. Alternatively spliced transcript variants encoding
            different isoforms have been found for this gene. [provided by
            RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) encodes isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803614.221732.1,
                                           SRR1803616.21965.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2145743, SAMEA2154665
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000285900.10/ ENSP00000285900.4
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-301               DC335107.1         148-448
            302-1342            AK310189.1         227-1267
            1343-2316           M81886.1           1267-2240
            2317-3037           X58633.1           2201-2921
            3038-3462           AB209094.1         3104-3528
            3463-5394           AB209094.1         3530-5461
            5395-5584           AL120214.1         80-269
FEATURES             Location/Qualifiers
     source          1..5584
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="5"
                     /map="5q33.2"
     gene            1..5584
                     /gene="GRIA1"
                     /gene_synonym="GluA1; GLUH1; GLUR1; GLURA; HBGR1; MRD67;
                     MRT76"
                     /note="glutamate ionotropic receptor AMPA type subunit 1"
                     /db_xref="GeneID:2890"
                     /db_xref="HGNC:HGNC:4571"
                     /db_xref="MIM:138248"
     exon            1..301
                     /gene="GRIA1"
                     /gene_synonym="GluA1; GLUH1; GLUR1; GLURA; HBGR1; MRD67;
                     MRT76"
                     /inference="alignment:Splign:2.1.0"
     CDS             220..2940
                     /gene="GRIA1"
                     /gene_synonym="GluA1; GLUH1; GLUR1; GLURA; HBGR1; MRD67;
                     MRT76"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; AMPA 1; gluR-1; gluR-A; gluR-K1; AMPA-selective
                     glutamate receptor 1; AMPA receptor subunit GluA1;
                     glutamate receptor, ionotropic, AMPA 1"
                     /codon_start=1
                     /product="glutamate receptor 1 isoform 1 precursor"
                     /protein_id="NP_000818.2"
                     /db_xref="CCDS:CCDS4322.1"
                     /db_xref="GeneID:2890"
                     /db_xref="HGNC:HGNC:4571"
                     /db_xref="MIM:138248"
                     /translation="MQHIFAFFCTGFLGAVVGANFPNNIQIGGLFPNQQSQEHAAFRF
                     ALSQLTEPPKLLPQIDIVNISDSFEMTYRFCSQFSKGVYAIFGFYERRTVNMLTSFCG
                     ALHVCFITPSFPVDTSNQFVLQLRPELQDALISIIDHYKWQKFVYIYDADRGLSVLQK
                     VLDTAAEKNWQVTAVNILTTTEEGYRMLFQDLEKKKERLVVVDCESERLNAILGQIIK
                     LEKNGIGYHYILANLGFMDIDLNKFKESGANVTGFQLVNYTDTIPAKIMQQWKNSDAR
                     DHTRVDWKRPKYTSALTYDGVKVMAEAFQSLRRQRIDISRRGNAGDCLANPAVPWGQG
                     IDIQRALQQVRFEGLTGNVQFNEKGRRTNYTLHVIEMKHDGIRKIGYWNEDDKFVPAA
                     TDAQAGGDNSSVQNRTYIVTTILEDPYVMLKKNANQFEGNDRYEGYCVELAAEIAKHV
                     GYSYRLEIVSDGKYGARDPDTKAWNGMVGELVYGRADVAVAPLTITLVREEVIDFSKP
                     FMSLGISIMIKKPQKSKPGVFSFLDPLAYEIWMCIVFAYIGVSVVLFLVSRFSPYEWH
                     SEEFEEGRDQTTSDQSNEFGIFNSLWFSLGAFMQQGCDISPRSLSGRIVGGVWWFFTL
                     IIISSYTANLAAFLTVERMVSPIESAEDLAKQTEIAYGTLEAGSTKEFFRRSKIAVFE
                     KMWTYMKSAEPSVFVRTTEEGMIRVRKSKGKYAYLLESTMNEYIEQRKPCDTMKVGGN
                     LDSKGYGIATPKGSALRNPVNLAVLKLNEQGLLDKLKNKWWYDKGECGSGGGDSKDKT
                     SALSLSNVAGVFYILIGGLGLAMLVALIEFCYKSRSESKRMKGFCLIPQQSINEAIRT
                     STLPRNSGAGASSGGSGENGRVVSHDFPKSMQSIPCMSHSSGMPLGATGL"
     exon            302..439
                     /gene="GRIA1"
                     /gene_synonym="GluA1; GLUH1; GLUR1; GLURA; HBGR1; MRD67;
                     MRT76"
                     /inference="alignment:Splign:2.1.0"
     exon            440..679
                     /gene="GRIA1"
                     /gene_synonym="GluA1; GLUH1; GLUR1; GLURA; HBGR1; MRD67;
                     MRT76"
                     /inference="alignment:Splign:2.1.0"
     exon            680..864
                     /gene="GRIA1"
                     /gene_synonym="GluA1; GLUH1; GLUR1; GLURA; HBGR1; MRD67;
                     MRT76"
                     /inference="alignment:Splign:2.1.0"
     exon            865..918
                     /gene="GRIA1"
                     /gene_synonym="GluA1; GLUH1; GLUR1; GLURA; HBGR1; MRD67;
                     MRT76"
                     /inference="alignment:Splign:2.1.0"
     exon            919..1080
                     /gene="GRIA1"
                     /gene_synonym="GluA1; GLUH1; GLUR1; GLURA; HBGR1; MRD67;
                     MRT76"
                     /inference="alignment:Splign:2.1.0"
     exon            1081..1248
                     /gene="GRIA1"
                     /gene_synonym="GluA1; GLUH1; GLUR1; GLURA; HBGR1; MRD67;
                     MRT76"
                     /inference="alignment:Splign:2.1.0"
     exon            1249..1353
                     /gene="GRIA1"
                     /gene_synonym="GluA1; GLUH1; GLUR1; GLURA; HBGR1; MRD67;
                     MRT76"
                     /inference="alignment:Splign:2.1.0"
     exon            1354..1464
                     /gene="GRIA1"
                     /gene_synonym="GluA1; GLUH1; GLUR1; GLURA; HBGR1; MRD67;
                     MRT76"
                     /inference="alignment:Splign:2.1.0"
     exon            1465..1671
                     /gene="GRIA1"
                     /gene_synonym="GluA1; GLUH1; GLUR1; GLURA; HBGR1; MRD67;
                     MRT76"
                     /inference="alignment:Splign:2.1.0"
     exon            1672..2042
                     /gene="GRIA1"
                     /gene_synonym="GluA1; GLUH1; GLUR1; GLURA; HBGR1; MRD67;
                     MRT76"
                     /inference="alignment:Splign:2.1.0"
     exon            2043..2241
                     /gene="GRIA1"
                     /gene_synonym="GluA1; GLUH1; GLUR1; GLURA; HBGR1; MRD67;
                     MRT76"
                     /inference="alignment:Splign:2.1.0"
     exon            2242..2489
                     /gene="GRIA1"
                     /gene_synonym="GluA1; GLUH1; GLUR1; GLURA; HBGR1; MRD67;
                     MRT76"
                     /inference="alignment:Splign:2.1.0"
     exon            2490..2604
                     /gene="GRIA1"
                     /gene_synonym="GluA1; GLUH1; GLUR1; GLURA; HBGR1; MRD67;
                     MRT76"
                     /inference="alignment:Splign:2.1.0"
     exon            2605..2739
                     /gene="GRIA1"
                     /gene_synonym="GluA1; GLUH1; GLUR1; GLURA; HBGR1; MRD67;
                     MRT76"
                     /inference="alignment:Splign:2.1.0"
     exon            2740..5584
                     /gene="GRIA1"
                     /gene_synonym="GluA1; GLUH1; GLUR1; GLURA; HBGR1; MRD67;
                     MRT76"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5375..5380
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GRIA1"
                     /gene_synonym="GluA1; GLUH1; GLUR1; GLURA; HBGR1; MRD67;
                     MRT76"
                     /note="hexamer: ATTAAA"
     polyA_site      5395
                     /gene="GRIA1"
                     /gene_synonym="GluA1; GLUH1; GLUR1; GLURA; HBGR1; MRD67;
                     MRT76"
                     /note="major polyA site"
     regulatory      5562..5567
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GRIA1"
                     /gene_synonym="GluA1; GLUH1; GLUR1; GLURA; HBGR1; MRD67;
                     MRT76"
                     /note="hexamer: ATTAAA"
     polyA_site      5584
                     /gene="GRIA1"
                     /gene_synonym="GluA1; GLUH1; GLUR1; GLURA; HBGR1; MRD67;
                     MRT76"
                     /note="major polyA site"
ORIGIN      
        1 agttaatctg gctgtcagtt ggtgttaacg ctgcagttta agtgttcgga ttccaaggga
       61 aacagacaaa cctcacgaaa ggaaggaagc aagcaagcaa ggaaggaact gcaggaggaa
      121 aagaacaggc agaacagcga gaagaataaa gggaaagggg gggaaacacc aaatctatga
      181 ttggacctgg gcttcttttt cgccaatgca aaaaggaata tgcagcacat ttttgccttc
      241 ttctgcaccg gtttcctagg cgcggtagta ggtgccaatt tccccaacaa tatccagatc
      301 gggggattat ttccaaacca gcagtcacag gaacatgctg cttttagatt tgctttgtcg
      361 caactcacag agcccccgaa gctgctcccc cagattgata ttgtgaacat cagcgacagc
      421 tttgagatga cctatagatt ctgttcccag ttctccaaag gagtctatgc catctttggg
      481 ttttatgaac gtaggactgt caacatgctg acctcctttt gtggggccct ccacgtctgc
      541 ttcattacgc cgagctttcc cgttgataca tccaatcagt ttgtccttca gctgcgccct
      601 gaactgcagg atgccctcat cagcatcatt gaccattaca agtggcagaa atttgtctac
      661 atttatgatg ccgaccgggg cttatccgtc ctgcagaaag tcctggatac agctgctgag
      721 aagaactggc aggtgacagc agtcaacatt ttgacaacca cagaggaggg ataccggatg
      781 ctctttcagg acctggagaa gaaaaaggag cggctggtgg tggtggactg tgaatcagaa
      841 cgcctcaatg ctatcttggg ccagattata aagctagaga agaatggcat cggctaccac
      901 tacattcttg caaatctggg cttcatggac attgacttaa acaaattcaa ggagagtggc
      961 gccaatgtga caggtttcca gctggtgaac tacacagaca ctattccggc caagatcatg
     1021 cagcagtgga agaatagtga tgctcgagac cacacacggg tggactggaa gagacccaag
     1081 tacacctctg cgctcaccta cgatggggtg aaggtgatgg ctgaggcttt ccagagcctg
     1141 cggaggcaga gaattgatat atctcgccgg gggaatgctg gggattgtct ggctaaccca
     1201 gctgttccct ggggccaagg gatcgacatc cagagagctc tgcagcaggt gcgatttgaa
     1261 ggtttaacag gaaacgtgca gtttaatgag aaaggacgcc ggaccaacta cacgctccac
     1321 gtgattgaaa tgaaacatga cggcatccga aagattggtt actggaatga agatgataag
     1381 tttgtccctg cagccaccga tgcccaagct gggggcgata attcaagtgt tcagaacaga
     1441 acatacatcg tcacaacaat cctagaagat ccttatgtga tgctcaagaa gaacgccaat
     1501 cagtttgagg gcaatgaccg ttacgagggc tactgtgtag agctggcggc agagattgcc
     1561 aagcacgtgg gctactccta ccgtctggag attgtcagtg atggaaaata cggagcccga
     1621 gaccctgaca cgaaggcctg gaatggcatg gtgggagagc tggtctatgg aagagcagat
     1681 gtggctgtgg ctcccttaac tatcactttg gtccgggaag aagttataga tttctccaaa
     1741 ccatttatga gtttggggat ctccatcatg attaaaaaac cacagaaatc caagccgggt
     1801 gtcttctcct tccttgatcc tttggcttat gagatttgga tgtgcattgt ttttgcctac
     1861 attggagtga gtgttgtcct cttcctggtc agccgcttca gtccctatga atggcacagt
     1921 gaagagtttg aggaaggacg ggaccagaca accagtgacc agtccaatga gtttgggata
     1981 ttcaacagtt tgtggttctc cctgggagcc ttcatgcagc aaggatgtga catttctccc
     2041 aggtccctgt ctggtcgcat cgttggtggc gtctggtggt tcttcacctt aatcatcatc
     2101 tcctcatata cagccaatct ggccgccttc ctgaccgtgg agaggatggt gtctcccatt
     2161 gagagtgcag aggacctagc gaagcagaca gaaattgcct acgggacgct ggaagcagga
     2221 tctactaagg agttcttcag gaggtctaaa attgctgtgt ttgagaagat gtggacatac
     2281 atgaagtcag cagagccatc agtttttgtg cggaccacag aggaggggat gattcgagtg
     2341 aggaaatcca aaggcaaata tgcctacctc ctggagtcca ccatgaatga gtacattgag
     2401 cagcggaaac cctgtgacac catgaaggtg ggaggtaact tggattccaa aggctatggc
     2461 attgcaacac ccaaggggtc tgccctgaga aatccagtaa acctggcagt gttaaaactg
     2521 aacgagcagg ggcttttgga caaattgaaa aacaaatggt ggtacgacaa gggcgagtgc
     2581 ggcagcgggg gaggtgattc caaggacaag acaagcgctc tgagcctcag caatgtggca
     2641 ggcgtgttct acatcctgat cggaggactt ggactagcca tgctggttgc cttaatcgag
     2701 ttctgctaca aatcccgtag tgaatccaag cggatgaagg gtttttgttt gatcccacag
     2761 caatccatca acgaagccat acggacatcg accctccccc gcaacagcgg ggcaggagcc
     2821 agcagcggcg gcagtggaga gaatggtcgg gtggtcagcc atgacttccc caagtccatg
     2881 caatcgattc cttgcatgag ccacagttca gggatgccct tgggagccac gggattgtaa
     2941 ctggagcaga tggagacccc ttggggagca ggctcgggct ccccagcccc atcccaaacc
     3001 cttcagtgcc aaaaacaaca acaaaatgaa acgcaaccac caccaaccac tgcgaccaca
     3061 agaaggatga ttcaacaggt tttcctgaag aattgaaaaa ccattttgct gtcccttttc
     3121 cttttttgat gttctttcac ccttttctgt ttgctaagtg aggatgaaaa aataacactg
     3181 tactgcaata aggggagagt aaccctgtct aatgaaacct gtgtctctga gagtagagtc
     3241 actggaacac taatgaggaa actgcactgt tttattttaa ttcagttgtt agtgtgtctt
     3301 agtgtgtgca attttttttc ttactaatat ccatggtttg caggttctgt taggcccttt
     3361 ccttctcctt acttcttatc cccaactccc tacccacccc tcttcagttt tcagattgga
     3421 gattcaagat ttgttccact ttacaagcaa gaggaaaaaa aagcaacctt caaactaatt
     3481 ctccatgggg gctctccatg ttaccctcca ctccttggcc caaacctctg atggagatag
     3541 acattgttgg agaagtgggc tgccttcccc aagtggggca ctgcttaagc acttattcag
     3601 tggagaacac aggtgaaaag caactcagga tgagggtggt ggagagggca ggggcagatg
     3661 tgcagtcaga gaaggactcc tgaagttact gctgctcaga aaaacagttc ctttaatgtg
     3721 gaagagccat ttcataggtc ataggtggta tggtatattt cttcagagtc aaccttggcc
     3781 ctgagaagta tgtcctcctg gtgtgctcag gctcaacggc agtctggtgg ctgaaggcac
     3841 ttggcctcct aaaccaagca gaattttggg aagagataac agccagggag atattgccca
     3901 tgattctcac tttttctttg cctggcatct aagcaggaac ccattgtgga gtagactctc
     3961 ttcttctatg gagcctctga catggggagc aatgctaagc aagctaagtg taaaagaaaa
     4021 gtgacagaat aattttggaa gaggaagcct catcaaaagc tcacacaaaa tagagcttcc
     4081 catggtgtgc cctatcctag gtttaagaaa acacgtatga agtttatgct gatgcaaaga
     4141 acttgggttt ttatgttaat ataaagtgtt gttttagcat gtggccagat gatgctctgt
     4201 catctttaga aagtgagata accaaggaaa taattgaagg agtataggga gatggattaa
     4261 gttgataatg acatttaggg caacttaaga cctttgatcc caggttctaa ctcaaagagg
     4321 ctgaccttcc cccagctaag atagcatgag gacgttgtat tccaatatac gtatgattgg
     4381 ggctacaaag ctgaactaaa gcaagattgg tgaagtggca gggtttatag agagaagccc
     4441 aggctgagtt cagcttttgt tggaagtgag aatccctgac atatagcttt cttggagatc
     4501 ccaactctca ttcttggtgc aactggcttc cagctctcca gcagtcactc tcctaggtgc
     4561 atgattcagt gcgtgccatg tgtcattagc ttttactgat aaccatattc tggcttgttc
     4621 ccttaccccc tacttctatc caattttctc tgctaggggt tatcattagc aattgacatg
     4681 ctaaaggttt tggagcccac ctaggggtag gtgcagcttt attggctttt ctgtggattc
     4741 tctcagtgga cccacaccat ctctatgtct ctccactctc ctgccttcag ccatagcaaa
     4801 gaatccttcc aaaatcaaac tcttcacttt tttgactcaa gtgttgttgt tcagtctctc
     4861 gcgtgtcaat gtggtcatgg ttcatgaaac cggaccctca agatggatga ttgcttttaa
     4921 ctactgccag ctgatgtctc tcagcccctg ccctcataca agatttttct cagccttcag
     4981 cctaccactg cagaatccga tgtgacccac cattagggag tctgcatctt ggaagagttg
     5041 gaaataaccc tttaacatca acatgcttca aagacttttt gcctttggcc tagtaagatg
     5101 cctctccagc tactgagccc acaagtaaca tgagcggata aaaagagact tgtttgtgct
     5161 agaaatgagg gtctatgcta tgagggggtc caagactctg gcgaaatgtg ctttttcatc
     5221 aatggagaaa tgaaaggaaa acacaagcaa gaaaaaagtt aacttgtatt atgtattttt
     5281 actacacttt tcttaaaaat agagcattgg gaaaactctg aaagagactg acatttttct
     5341 caacaggaat ccatacttaa cagttctggc tttcattaaa ttttgctctt tggtacctgg
     5401 gccttttatt taacatctat atttgtttta actctcttgg cagatgtgtg aaaggattct
     5461 tgcttgatca aacactaagt atttttttgg ttcttgtttt tctttcaaat agccaggttt
     5521 ttttcttttg gtatttgcat aaaatgaaaa tatcaccgaa tattaaatca ctgtggatcc
     5581 atta
//
