LOCUS       NM_002086               3273 bp    mRNA    linear   PRI 20-FEB-2025
DEFINITION  Homo sapiens growth factor receptor bound protein 2 (GRB2),
            transcript variant 1, mRNA.
ACCESSION   NM_002086 NM_025199
VERSION     NM_002086.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3273)
  AUTHORS   Li,Z., Zhao,P.L., Gao,X., Li,X., Meng,Y.Q., Li,Z.Q., Zhai,K.R.,
            Wei,S.L., Feng,H.M., Huang,H.R. and Li,B.
  TITLE     DUS4L suppresses invasion and metastasis in LUAD via modulation of
            PI3K/AKT and ERK/MAPK signaling through GRB2
  JOURNAL   Int Immunopharmacol 142 (Pt A), 113043 (2024)
   PUBMED   39216120
  REMARK    GeneRIF: DUS4L suppresses invasion and metastasis in LUAD via
            modulation of PI3K/AKT and ERK/MAPK signaling through GRB2.
REFERENCE   2  (bases 1 to 3273)
  AUTHORS   Khoroshkin,M., Buyan,A., Dodel,M., Navickas,A., Yu,J., Trejo,F.,
            Doty,A., Baratam,R., Zhou,S., Lee,S.B., Joshi,T., Garcia,K.,
            Choi,B., Miglani,S., Subramanyam,V., Modi,H., Carpenter,C.,
            Markett,D., Corces,M.R., Mardakheh,F.K., Kulakovskiy,I.V. and
            Goodarzi,H.
  TITLE     Systematic identification of post-transcriptional regulatory
            modules
  JOURNAL   Nat Commun 15 (1), 7872 (2024)
   PUBMED   39251607
  REMARK    Erratum:[Nat Commun. 2024 Oct 28;15(1):9277. doi:
            10.1038/s41467-024-52903-4.. PMID: 39468017]
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3273)
  AUTHORS   Tang,A.A.S., Macdonald,A., McPherson,M.J. and Tomlinson,D.C.
  TITLE     Targeting Grb2 SH3 Domains with Affimer Proteins Provides Novel
            Insights into Ras Signalling Modulation
  JOURNAL   Biomolecules 14 (8), 1040 (2024)
   PUBMED   39199427
  REMARK    GeneRIF: Targeting Grb2 SH3 Domains with Affimer Proteins Provides
            Novel Insights into Ras Signalling Modulation.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3273)
  AUTHORS   Qiu,T., Kong,Y., Wei,G., Sun,K., Wang,R., Wang,Y., Chen,Y.,
            Wang,W., Zhang,Y., Jiang,C., Yang,P., Xie,T. and Chen,X.
  TITLE     CCDC6-RET fusion protein regulates Ras/MAPK signaling through the
            fusion- GRB2-SHC1 signal niche
  JOURNAL   Proc Natl Acad Sci U S A 121 (23), e2322359121 (2024)
   PUBMED   38805286
  REMARK    GeneRIF: CCDC6-RET fusion protein regulates Ras/MAPK signaling
            through the fusion- GRB2-SHC1 signal niche.
REFERENCE   5  (bases 1 to 3273)
  AUTHORS   Ye,Z., Xu,S., Shi,Y., Cheng,X., Zhang,Y., Roy,S., Namjoshi,S.,
            Longo,M.A., Link,T.M., Schlacher,K., Peng,G., Yu,D., Wang,B.,
            Tainer,J.A. and Ahmed,Z.
  TITLE     GRB2 stabilizes RAD51 at reversed replication forks suppressing
            genomic instability and innate immunity against cancer
  JOURNAL   Nat Commun 15 (1), 2132 (2024)
   PUBMED   38459011
  REMARK    GeneRIF: GRB2 stabilizes RAD51 at reversed replication forks
            suppressing genomic instability and innate immunity against cancer.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3273)
  AUTHORS   Huang,L., Turck,C.W., Rao,P. and Peters,K.G.
  TITLE     GRB2 and SH-PTP2: potentially important endothelial signaling
            molecules downstream of the TEK/TIE2 receptor tyrosine kinase
  JOURNAL   Oncogene 11 (10), 2097-2103 (1995)
   PUBMED   7478529
REFERENCE   7  (bases 1 to 3273)
  AUTHORS   Meisner,H. and Czech,M.P.
  TITLE     A 115 kDa tyrosine phosphorylated protein associates with Grb-2 in
            activated Jurkat cells
  JOURNAL   Ann N Y Acad Sci 766, 204-205 (1995)
   PUBMED   7486658
REFERENCE   8  (bases 1 to 3273)
  AUTHORS   Matuoka,K., Shibata,M., Yamakawa,A. and Takenawa,T.
  TITLE     Cloning of ASH, a ubiquitous protein composed of one Src homology
            region (SH) 2 and two SH3 domains, from human and rat cDNA
            libraries
  JOURNAL   Proc Natl Acad Sci U S A 89 (19), 9015-9019 (1992)
   PUBMED   1384039
REFERENCE   9  (bases 1 to 3273)
  AUTHORS   Lowenstein,E.J., Daly,R.J., Batzer,A.G., Li,W., Margolis,B.,
            Lammers,R., Ullrich,A., Skolnik,E.Y., Bar-Sagi,D. and
            Schlessinger,J.
  TITLE     The SH2 and SH3 domain-containing protein GRB2 links receptor
            tyrosine kinases to ras signaling
  JOURNAL   Cell 70 (3), 431-442 (1992)
   PUBMED   1322798
REFERENCE   10 (bases 1 to 3273)
  AUTHORS   Jagielski,J. and Pruszynski,B.
  TITLE     [A case of adrenal adenoma diagnosed by means of adrenal
            phlebography (author's transl)]
  JOURNAL   Pol Przegl Radiol Med Nukl 41 (4), 291-292 (1977)
   PUBMED   917890
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC011933.17.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_002086.4.
            
            Summary: The protein encoded by this gene binds the epidermal
            growth factor receptor and contains one SH2 domain and two SH3
            domains. Its two SH3 domains direct complex formation with
            proline-rich regions of other proteins, and its SH2 domain binds
            tyrosine phosphorylated sequences. This gene is similar to the Sem5
            gene of C.elegans, which is involved in the signal transduction
            pathway. Two alternatively spliced transcript variants encoding
            different isoforms have been found for this gene. [provided by
            RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: DRR138522.646033.1, BC000631.2
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968540, SAMEA2142348
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000316804.10/ ENSP00000339007.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-190               AC011933.17        21031-21220
            191-405             AC011933.17        32944-33158
            406-503             AC011933.17        93912-94009
            504-626             AC011933.17        100689-100811
            627-795             AC011933.17        104882-105050
            796-3273            AC011933.17        106156-108633
FEATURES             Location/Qualifiers
     source          1..3273
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17q25.1"
     gene            1..3273
                     /gene="GRB2"
                     /gene_synonym="ASH; EGFRBP-GRB2; Grb3-3; MST084; MSTP084;
                     NCKAP2"
                     /note="growth factor receptor bound protein 2"
                     /db_xref="GeneID:2885"
                     /db_xref="HGNC:HGNC:4566"
                     /db_xref="MIM:108355"
     exon            1..190
                     /gene="GRB2"
                     /gene_synonym="ASH; EGFRBP-GRB2; Grb3-3; MST084; MSTP084;
                     NCKAP2"
                     /inference="alignment:Splign:2.1.0"
     exon            191..405
                     /gene="GRB2"
                     /gene_synonym="ASH; EGFRBP-GRB2; Grb3-3; MST084; MSTP084;
                     NCKAP2"
                     /inference="alignment:Splign:2.1.0"
     CDS             328..981
                     /gene="GRB2"
                     /gene_synonym="ASH; EGFRBP-GRB2; Grb3-3; MST084; MSTP084;
                     NCKAP2"
                     /note="isoform 1 is encoded by transcript variant 1;
                     HT027; growth factor receptor-bound protein 3; epidermal
                     growth factor receptor-binding protein GRB2; abundant SRC
                     homology; protein Ash; SH2/SH3 adapter GRB2; epididymis
                     secretory sperm binding protein"
                     /codon_start=1
                     /product="growth factor receptor-bound protein 2 isoform
                     1"
                     /protein_id="NP_002077.1"
                     /db_xref="CCDS:CCDS11721.1"
                     /db_xref="GeneID:2885"
                     /db_xref="HGNC:HGNC:4566"
                     /db_xref="MIM:108355"
                     /translation="MEAIAKYDFKATADDELSFKRGDILKVLNEECDQNWYKAELNGK
                     DGFIPKNYIEMKPHPWFFGKIPRAKAEEMLSKQRHDGAFLIRESESAPGDFSLSVKFG
                     NDVQHFKVLRDGAGKYFLWVVKFNSLNELVDYHRSTSVSRNQQIFLRDIEQVPQQPTY
                     VQALFDFDPQEDGELGFRRGDFIHVMDNSDPNWWKGACHGQTGMFPRNYVTPVNRNV"
     exon            406..503
                     /gene="GRB2"
                     /gene_synonym="ASH; EGFRBP-GRB2; Grb3-3; MST084; MSTP084;
                     NCKAP2"
                     /inference="alignment:Splign:2.1.0"
     exon            504..626
                     /gene="GRB2"
                     /gene_synonym="ASH; EGFRBP-GRB2; Grb3-3; MST084; MSTP084;
                     NCKAP2"
                     /inference="alignment:Splign:2.1.0"
     exon            627..795
                     /gene="GRB2"
                     /gene_synonym="ASH; EGFRBP-GRB2; Grb3-3; MST084; MSTP084;
                     NCKAP2"
                     /inference="alignment:Splign:2.1.0"
     exon            796..3273
                     /gene="GRB2"
                     /gene_synonym="ASH; EGFRBP-GRB2; Grb3-3; MST084; MSTP084;
                     NCKAP2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1319..1324
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GRB2"
                     /gene_synonym="ASH; EGFRBP-GRB2; Grb3-3; MST084; MSTP084;
                     NCKAP2"
                     /note="hexamer: AATAAA"
     polyA_site      1338
                     /gene="GRB2"
                     /gene_synonym="ASH; EGFRBP-GRB2; Grb3-3; MST084; MSTP084;
                     NCKAP2"
     regulatory      3250..3255
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GRB2"
                     /gene_synonym="ASH; EGFRBP-GRB2; Grb3-3; MST084; MSTP084;
                     NCKAP2"
                     /note="hexamer: AATAAA"
     polyA_site      3273
                     /gene="GRB2"
                     /gene_synonym="ASH; EGFRBP-GRB2; Grb3-3; MST084; MSTP084;
                     NCKAP2"
                     /note="major polyA site"
ORIGIN      
        1 agccaggagg tattgctgct tcggcgaccg ggcggcggca gcggcggcgg cggctgtggc
       61 agagtctgtg cctgtggcgg tgacggcggc gggagcaagc gctgccctcg cagagcagcc
      121 ttggggtcgc cggccgctcg cagcgttgtg gaggggcggg ccggacgctg agcggagcag
      181 ctgcgccacg ggtggcattg tgtgtcccag agtgccggag cgagtcccag aagagaggcg
      241 aggctaagcc cagagcgctg ggttgcttca gcagggaaga ctcccttccc cctgcttcag
      301 gctgctgagc actgagcagc gctcagaatg gaagccatcg ccaaatatga cttcaaagct
      361 actgcagacg acgagctgag cttcaaaagg ggggacatcc tcaaggtttt gaacgaagaa
      421 tgtgatcaga actggtacaa ggcagagctt aatggaaaag acggcttcat tcccaagaac
      481 tacatagaaa tgaaaccaca tccgtggttt tttggcaaaa tccccagagc caaggcagaa
      541 gaaatgctta gcaaacagcg gcacgatggg gcctttctta tccgagagag tgagagcgct
      601 cctggggact tctccctctc tgtcaagttt ggaaacgatg tgcagcactt caaggtgctc
      661 cgagatggag ccgggaagta cttcctctgg gtggtgaagt tcaattcttt gaatgagctg
      721 gtggattatc acagatctac atctgtctcc agaaaccagc agatattcct gcgggacata
      781 gaacaggtgc cacagcagcc gacatacgtc caggccctct ttgactttga tccccaggag
      841 gatggagagc tgggcttccg ccggggagat tttatccatg tcatggataa ctcagacccc
      901 aactggtgga aaggagcttg ccacgggcag accggcatgt ttccccgcaa ttatgtcacc
      961 cccgtgaacc ggaacgtcta agagtcaaga agcaattatt taaagaaagt gaaaaatgta
     1021 aaacacatac aaaagaatta aacccacaag ctgcctctga cagcagcctg tgagggagtg
     1081 cagaacacct ggccgggtca ccctgtgacc ctctcacttt ggttggaact ttagggggtg
     1141 ggagggggcg ttggatttaa aaatgccaaa acttacctat aaattaagaa gagtttttat
     1201 tacaaatttt cactgctgct cctctttccc ctcctttgtc ttttttttca tccttttttc
     1261 tcttctgtcc atcagtgcat gacgtttaag gccacgtata gtcctagctg acgccaataa
     1321 taaaaaacaa gaaaccaagt gggctggtat tctctctatg caaaatgtct gttttagttg
     1381 gaatgactga aagaagaaca gctgttcctg tgttcttcgt atatacacac aaaaaggagc
     1441 gggcagggcc gctcgatgcc tttgctgttt agcttcctcc agaggagggg acttgtagga
     1501 atctgccttc cagcccagac ccccagtgta ttttgtccaa gttcacagta gagtagggta
     1561 gaaggaaagc atgtctctgc ttccatggct tcctgagaaa gcccacctgg gctgggcgcg
     1621 gtggctcacg cctgtaatcc cagcactttg ggaggccaag gtgggcggat cacaaggtca
     1681 ggagttcgag accaacctag ccaacatggt gaaaccccgt ctctactaaa aataagaaat
     1741 tagccgggtg tggcacgcac ctgtagtccc agctacttgg gagcctgagg caggagaatc
     1801 gcttgaacct gggaagtgga ggttgagtga gccgggaccg tgccattgta ctccagcctg
     1861 ggtgacagag cgagattccg tctcaaaaaa aaaaaaaaaa agcccacctg aaagcctgtc
     1921 tctttccact ttgttggccc ttccagtggg attatcgagc atgttgtttt ttcatagtgc
     1981 ctttttcctt atttcaaggg ttgcttctga gtggtgtttt tttttttttt ttaatttgtt
     2041 ttgttttaaa ataagttaaa ggcagtccag agcttttcag ccaatttgtc tcctactctg
     2101 tgtaaatatt tttccctccg ggcaggggag ccagggtaga gcaaaggaga caaagcagga
     2161 gtggaaggtg aggcgttctc ctgcttgtac taagccagga ggctttaagc tccagcttta
     2221 agggttgtga gccccttggg ggttcaggga actgcttgcc cagggtgcag tgtgagtgtg
     2281 atgggccacc ggggcaagag ggaaggtgac cgcccagctc tcccacatcc cactggatct
     2341 ggcttacagg ggggtcggaa gcctgtcctc accgtctcgg gggttgtggc ccccgccccc
     2401 tccctatatg cacccctgga accagcaagt cccagacaag gagagcggag gaggaagtca
     2461 tgggaacgca gcctccagtt gtagcaggtt tcactattcc tatgctgggg tacacagtga
     2521 gagtactcac ttttcacttg tcttgctctt agattgggcc atggctttca tcctgtgtcc
     2581 cctgacctgt ccaggtgagt gtgagggcag cactgggaag ctggagtgct gcttgtgcct
     2641 cccttcccag tgggctgtgt tgactgctgc tccccacccc taccgatggt cccaggaagc
     2701 agggagagtt ggggaaggca agattggaaa gacaggaaga ccaaggcctc ggcagaactc
     2761 tctgtcttct ctccacttct ggtcccctgt ggtgatgtgc ctgtaatctt tttctccacc
     2821 caaacccctt cccacgacaa aaacaagact gcctccctct cttccgggag ctggtgacag
     2881 ccttgggcct ttcagtccca aagcggccga tgggagtctc cctccgactc cagatatgaa
     2941 cagggcccag gcctggagcg tttgctgtgc caggaggcgg cagctcttct gggcagagcc
     3001 tgtccccgcc ttccctcact cttcctcatc ctgcttctct tttcctcgca gatgataaaa
     3061 ggaatctggc attctacacc tggaccattt gattgtttta ttttggaatt ggtgtatatc
     3121 atgaagcctt gctgaactaa gttttgtgtg tatatattta aaaaaaaaat cagtgtttaa
     3181 ataaagacct atgtacttaa tcctttaact ctgcggatag catttggtag gtagtgatta
     3241 actgtgaata ataaatacac aatgaattct tca
//
