LOCUS       NM_004490               2415 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens growth factor receptor bound protein 14 (GRB14),
            transcript variant 1, mRNA.
ACCESSION   NM_004490 XM_939493 XM_944164 XM_944166 XM_944172 XM_944177
VERSION     NM_004490.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2415)
  AUTHORS   Sun,C., Forster,F., Gutsmann,B., Moulla,Y., Stroh,C., Dietrich,A.,
            Schon,M.R., Gartner,D., Lohmann,T., Dressler,M., Stumvoll,M.,
            Bluher,M., Kovacs,P., Breitfeld,J. and Guiu-Jurado,E.
  TITLE     Metabolic Effects of the Waist-To-Hip Ratio Associated Locus
            GRB14/COBLL1 Are Related to GRB14 Expression in Adipose Tissue
  JOURNAL   Int J Mol Sci 23 (15), 8558 (2022)
   PUBMED   35955692
  REMARK    GeneRIF: Metabolic Effects of the Waist-To-Hip Ratio Associated
            Locus GRB14/COBLL1 Are Related to GRB14 Expression in Adipose
            Tissue.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2415)
  AUTHORS   Wang,X., Cao,Q., Shi,Y., Wu,X., Mi,Y., Liu,K., Kan,Q., Fan,R.,
            Liu,Z. and Zhang,M.
  TITLE     Identification of low-dose radiation-induced exosomal circ-METRN
            and miR-4709-3p/GRB14/PDGFRalpha pathway as a key regulatory
            mechanism in Glioblastoma progression and radioresistance:
            Functional validation and clinical theranostic significance
  JOURNAL   Int J Biol Sci 17 (4), 1061-1078 (2021)
   PUBMED   33867829
  REMARK    GeneRIF: Identification of low-dose radiation-induced exosomal
            circ-METRN and miR-4709-3p/GRB14/PDGFRalpha pathway as a key
            regulatory mechanism in Glioblastoma progression and
            radioresistance: Functional validation and clinical theranostic
            significance.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2415)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 2415)
  AUTHORS   Taira,J., Yoshida,K., Takemoto,M., Hanada,K. and Sakamoto,H.
  TITLE     Dephosphorylation of clustered phosphoserine residues in human
            Grb14 by protein phosphatase 1 and its effect on insulin receptor
            complex formation
  JOURNAL   J Pept Sci 25 (10), e3207 (2019)
   PUBMED   31347216
  REMARK    GeneRIF: These findings suggested that phosphate groups on Ser(358)
            and Ser(362) in hGrb14 are dephosphorylated by PP1, and the
            dephosphorylation facilitates hGrb14-IR complex formation.
REFERENCE   5  (bases 1 to 2415)
  AUTHORS   Garcia-Palmero,I., Pompas-Veganzones,N., Villalobo,E., Gioria,S.,
            Haiech,J. and Villalobo,A.
  TITLE     The adaptors Grb10 and Grb14 are calmodulin-binding proteins
  JOURNAL   FEBS Lett 591 (8), 1176-1186 (2017)
   PUBMED   28295264
  REMARK    GeneRIF: Data suggest that GRB10 and GRB14 are both Ca2+-dependent
            CaM-binding proteins; more than one CaM-binding site and/or
            accessory CaM-binding sites appear to exist in GRB10 and GRB14, as
            compared to a single one present in GRB7. (GRB10 = growth factor
            receptor-bound protein 10; GRB14 = growth factor receptor-bound
            protein 14; CaM = calmodulin; GRB7 = growth factor receptor-bound
            protein 7)
REFERENCE   6  (bases 1 to 2415)
  AUTHORS   Jones,N., Master,Z., Jones,J., Bouchard,D., Gunji,Y., Sasaki,H.,
            Daly,R., Alitalo,K. and Dumont,D.J.
  TITLE     Identification of Tek/Tie2 binding partners. Binding to a
            multifunctional docking site mediates cell survival and migration
  JOURNAL   J Biol Chem 274 (43), 30896-30905 (1999)
   PUBMED   10521483
REFERENCE   7  (bases 1 to 2415)
  AUTHORS   Kasus-Jacobi,A., Perdereau,D., Auzan,C., Clauser,E., Van
            Obberghen,E., Mauvais-Jarvis,F., Girard,J. and Burnol,A.F.
  TITLE     Identification of the rat adapter Grb14 as an inhibitor of insulin
            actions
  JOURNAL   J Biol Chem 273 (40), 26026-26035 (1998)
   PUBMED   9748281
REFERENCE   8  (bases 1 to 2415)
  AUTHORS   Dong,L.Q., Du,H., Porter,S.G., Kolakowski,L.F. Jr., Lee,A.V.,
            Mandarino,L.J., Fan,J., Yee,D. and Liu,F.
  TITLE     Cloning, chromosome localization, expression, and characterization
            of an Src homology 2 and pleckstrin homology domain-containing
            insulin receptor binding protein hGrb10gamma
  JOURNAL   J Biol Chem 272 (46), 29104-29112 (1997)
   PUBMED   9360986
  REMARK    Erratum:[J Biol Chem 1998 Feb 13;273(7):4288. Mandarino J
            [corrected to Mandarino LJ]]
REFERENCE   9  (bases 1 to 2415)
  AUTHORS   Baker,E., Sutherland,G.R., Sutherland,R.L. and Daly,R.J.
  TITLE     Assignment of the human GRB14 gene to chromosome 2q22-q24 by
            fluorescence in situ hybridization
  JOURNAL   Genomics 36 (1), 218-220 (1996)
   PUBMED   8812444
REFERENCE   10 (bases 1 to 2415)
  AUTHORS   Daly,R.J., Sanderson,G.M., Janes,P.W. and Sutherland,R.L.
  TITLE     Cloning and characterization of GRB14, a novel member of the GRB7
            gene family
  JOURNAL   J Biol Chem 271 (21), 12502-12510 (1996)
   PUBMED   8647858
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC107075.4, BC053559.1 and
            AC110086.3.
            This sequence is a reference standard in the RefSeqGene project.
            
            On May 17, 2019 this sequence version replaced NM_004490.2.
            
            Summary: The product of this gene belongs to a small family of
            adapter proteins that are known to interact with a number of
            receptor tyrosine kinases and signaling molecules. This gene
            encodes a growth factor receptor-binding protein that interacts
            with insulin receptors and insulin-like growth-factor receptors.
            This protein likely has an inhibitory effect on receptor tyrosine
            kinase signaling and, in particular, on insulin receptor signaling.
            This gene may play a role in signaling pathways that regulate
            growth and metabolism. Alternative splicing results in multiple
            transcript variants. [provided by RefSeq, Dec 2014].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803613.267463.1,
                                           SRR1803612.265908.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1968189,
                                           SAMEA1968540 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000263915.8/ ENSP00000263915.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-162               AC107075.4         123851-124012       c
            163-451             BC053559.1         1-289
            452-452             AC107075.4         122262-122262       c
            453-2019            BC053559.1         291-1857
            2020-2415           AC110086.3         99852-100247        c
FEATURES             Location/Qualifiers
     source          1..2415
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q24.3"
     gene            1..2415
                     /gene="GRB14"
                     /note="growth factor receptor bound protein 14"
                     /db_xref="GeneID:2888"
                     /db_xref="HGNC:HGNC:4565"
                     /db_xref="MIM:601524"
     exon            1..364
                     /gene="GRB14"
                     /inference="alignment:Splign:2.1.0"
     CDS             174..1796
                     /gene="GRB14"
                     /note="isoform 1 is encoded by transcript variant 1; GRB14
                     adapter protein"
                     /codon_start=1
                     /product="growth factor receptor-bound protein 14 isoform
                     1"
                     /protein_id="NP_004481.2"
                     /db_xref="CCDS:CCDS2222.1"
                     /db_xref="GeneID:2888"
                     /db_xref="HGNC:HGNC:4565"
                     /db_xref="MIM:601524"
                     /translation="MTTSLQDGQSAASRAAARDSPLAAQVCGAAQGRGDAHDLAPAPW
                     LHARALLPLPDGTRGCAADRRKKKDLDVPEMPSIPNPFPELCCSPFTSVLSADLFPKA
                     NSRKKQVIKVYSEDETSRALDVPSDITARDVCQLLILKNHYIDDHSWTLFEHLPHIGV
                     ERTIEDHELVIEVLSNWGIEEENKLYFRKNYAKYEFFKNPMYFFPEHMVSFATETNGE
                     ISPTQILQMFLSSSTYPEIHGFLHAKEQGKKSWKKIYFFLRRSGLYFSTKGTSKEPRH
                     LQFFSEFGNSDIYVSLAGKKKHGAPTNYGFCFKPNKAGGPRDLKMLCAEEEQSRTCWV
                     TAIRLLKYGMQLYQNYMHPYQGRSGCSSQSISPMRSISENSLVAMDFSGQKSRVIENP
                     TEALSVAVEEGLAWRKKGCLRLGTHGSPTASSQSSATNMAIHRSQPWFHHKISRDEAQ
                     RLIIQQGLVDGVFLVRDSQSNPKTFVLSMSHGQKIKHFQIIPVEDDGEMFHTLDDGHT
                     RFTDLIQLVEFYQLNKGVLPCKLKHYCARIAL"
     exon            365..497
                     /gene="GRB14"
                     /inference="alignment:Splign:2.1.0"
     exon            498..654
                     /gene="GRB14"
                     /inference="alignment:Splign:2.1.0"
     exon            655..776
                     /gene="GRB14"
                     /inference="alignment:Splign:2.1.0"
     exon            777..851
                     /gene="GRB14"
                     /inference="alignment:Splign:2.1.0"
     exon            852..989
                     /gene="GRB14"
                     /inference="alignment:Splign:2.1.0"
     exon            990..1100
                     /gene="GRB14"
                     /inference="alignment:Splign:2.1.0"
     exon            1101..1196
                     /gene="GRB14"
                     /inference="alignment:Splign:2.1.0"
     exon            1197..1277
                     /gene="GRB14"
                     /inference="alignment:Splign:2.1.0"
     exon            1278..1394
                     /gene="GRB14"
                     /inference="alignment:Splign:2.1.0"
     exon            1395..1467
                     /gene="GRB14"
                     /inference="alignment:Splign:2.1.0"
     exon            1468..1555
                     /gene="GRB14"
                     /inference="alignment:Splign:2.1.0"
     exon            1556..1649
                     /gene="GRB14"
                     /inference="alignment:Splign:2.1.0"
     exon            1650..2415
                     /gene="GRB14"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1997..2002
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GRB14"
                     /note="hexamer: ATTAAA"
     polyA_site      2016
                     /gene="GRB14"
                     /note="major polyA site"
     regulatory      2141..2146
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GRB14"
                     /note="hexamer: AATAAA"
     polyA_site      2163
                     /gene="GRB14"
     regulatory      2391..2396
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GRB14"
                     /note="hexamer: ATTAAA"
     polyA_site      2415
                     /gene="GRB14"
ORIGIN      
        1 gcagatagct cggccgcgcg tctcagccgc cggggccccg agcgcaggcg gcgaggccac
       61 cacacctgca gagcgctcgg gctgcctagc cggcacctcg cctcccgccg cgcaaacccc
      121 ttctccccac gcgccgagtc tcccatgacg cccgagcccc ccggccggcg acaatgacca
      181 cttccctgca agatgggcag agcgccgcga gcagggcggc tgcccgggat tcgccgctgg
      241 ccgcccaggt gtgtggcgct gcccagggga ggggcgacgc ccacgacctg gcgccggccc
      301 cctggctgca cgcgcgagcg ctcctgcccc ttccggacgg gacccgcggc tgtgctgcag
      361 acaggagaaa aaagaaagat cttgatgttc cggaaatgcc atctattcca aacccttttc
      421 ctgagctatg ctgttctcca tttacatctg tgttgtcagc agacctattt cccaaagcaa
      481 attcaaggaa aaaacaggtg attaaagtat acagtgaaga tgaaaccagc agggctttag
      541 atgtacccag tgacataacg gctcgagatg tttgtcagct gttgatcctg aagaatcatt
      601 acattgatga ccacagctgg accctttttg agcacctgcc tcacataggt gtagaaagaa
      661 caatagaaga ccacgaactg gtgattgaag tgctatccaa ctgggggata gaagaagaaa
      721 acaaactata ctttagaaaa aattatgcca aatatgagtt ctttaaaaac ccaatgtatt
      781 tttttccaga gcatatggta tcttttgcaa ctgaaaccaa tggtgaaata tcccccacac
      841 agattttgca gatgtttctg agttcaagca catatcctga aattcatggt ttcttacatg
      901 cgaaagaaca gggaaagaag tcttggaaaa aaatttactt ttttctaaga agatctggtt
      961 tatatttttc tactaaagga acatcaaagg aaccgcggca tttgcagttt ttcagcgaat
     1021 ttggcaatag tgatatttat gtgtcactgg caggcaaaaa aaaacatgga gcaccgacta
     1081 actatggatt ctgctttaag cctaacaaag cgggagggcc ccgagacctg aaaatgctct
     1141 gtgcagaaga agagcagagt aggacgtgct gggtgaccgc gattagattg cttaagtatg
     1201 gcatgcagct gtaccagaat tatatgcatc catatcaagg tagaagtggc tgcagttcac
     1261 agagcatatc acctatgaga agtatatcag agaattccct ggtagcaatg gacttctcag
     1321 gccagaaaag cagagttata gaaaatccca ctgaagccct ttcagttgcg gttgaagaag
     1381 gactcgcttg gaggaaaaaa ggatgtttac gcctgggcac tcacggtagc cccactgcct
     1441 cttcacagag ctctgccaca aacatggcta tccaccggtc ccagccatgg tttcaccaca
     1501 aaatttctag agatgaggct cagcgattga ttattcagca aggacttgtg gatggagttt
     1561 tcttggtacg ggatagtcag agtaacccca aaactttcgt actgtcaatg agtcatggac
     1621 aaaaaataaa gcactttcaa attataccag tagaagatga cggtgaaatg ttccacacac
     1681 tggatgatgg ccacacaaga tttacagatc taatacagct ggtggagttc tatcaactca
     1741 ataagggcgt tcttccttgc aagttgaaac attattgtgc taggattgct ctctagacaa
     1801 gccagaagtg acttattaaa ctattgaagg aaaaggactc aagaaaaata ataaaagacc
     1861 ataaataagg gcgaaaacat taccatgtga aaagaatgta tttcacctgc aagttacaaa
     1921 aaaatagttt gtgcattgca aataagcaaa gacttggatt gactttacat tcatcattta
     1981 aaattcatta gttaaaatta aaccttagga aaaaaatgac ttggtgtgtt cttgtgtgat
     2041 ttttacattg ctataatatt ttcttcaaat atgcatattt aaaacatgtc tcccttattt
     2101 accatatagc aacatcagaa ttctgaaaca caaaatatga aataaattgg gagtcaggaa
     2161 taattatttg tgcactaaat ttcaattata ctatttttta atgttaagaa atatgataat
     2221 aacttatcaa ttaaaagaaa ctatccacaa cctgttaatg aaactaaaaa ctattttgaa
     2281 ttatatattt tccgggttta tgatcatctt aaaatgaaat catattttga gatagtagct
     2341 ttaaaccatt ttgaaatatg ttgatttttt ccaggtaatt taaaaatatt attaaatagt
     2401 taattataaa attta
//
