LOCUS       NM_015124               4500 bp    mRNA    linear   PRI 01-JUN-2024
DEFINITION  Homo sapiens GRAM domain containing 4 (GRAMD4), transcript variant
            1, mRNA.
ACCESSION   NM_015124 XM_005261398 XM_027105
VERSION     NM_015124.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4500)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   2  (bases 1 to 4500)
  AUTHORS   Zhang,J., Tu,K., Zhou,Z., Zheng,X., Gao,J., Yao,Y. and Liu,Q.
  TITLE     [Expression and clinicopathological significance of GRAMD4 in
            hepatocellular carcinoma]
  JOURNAL   Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 29 (2), 190-193 (2013)
   PUBMED   23388340
  REMARK    GeneRIF: The expression of GRAMD4 is up-regulated in hepatocellular
            carcinoma cell(HCC cell) lines and HCC tissues, and the increased
            expression is correlated with the clinicopathological
            characteristics of HCC.
REFERENCE   3  (bases 1 to 4500)
  AUTHORS   John,K., Alla,V., Meier,C. and Putzer,B.M.
  TITLE     GRAMD4 mimics p53 and mediates the apoptotic function of p73 at
            mitochondria
  JOURNAL   Cell Death Differ 18 (5), 874-886 (2011)
   PUBMED   21127500
  REMARK    GeneRIF: GRAMD4 induces changes in Bcl-2 and Bax protein levels
            Erratum:[Cell Death Differ. 2012 Mar;19(3):552]
REFERENCE   4  (bases 1 to 4500)
  AUTHORS   Stanelle,J., Tu-Rapp,H. and Putzer,B.M.
  TITLE     A novel mitochondrial protein DIP mediates E2F1-induced apoptosis
            independently of p53
  JOURNAL   Cell Death Differ 12 (4), 347-357 (2005)
   PUBMED   15565177
  REMARK    GeneRIF: localizes to the mitochondria, upregulated following E2F1
            induction
            GeneRIF: DIP induces p53-independent caspase-dependent and
            -independent apoptosis. DIP is localized in the mitochondria. DIP
            accumulates upon E2F1 activation.
REFERENCE   5  (bases 1 to 4500)
  AUTHORS   Stanelle,J., Stiewe,T., Theseling,C.C., Peter,M. and Putzer,B.M.
  TITLE     Gene expression changes in response to E2F1 activation
  JOURNAL   Nucleic Acids Res 30 (8), 1859-1867 (2002)
   PUBMED   11937641
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL021392.5 and AL096766.12.
            
            On Oct 13, 2018 this sequence version replaced NM_015124.4.
            
            Summary: GRAMD4 is a mitochondrial effector of E2F1 (MIM
            189971)-induced apoptosis (Stanelle et al., 2005 [PubMed
            15565177]).[supplied by OMIM, Jan 2011].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB018310.2 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1966682, SAMEA1968540
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000406902.6/ ENSP00000385689.1
            RefSeq Select criteria :: based on conservation
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-180               AL021392.5         27668-27847         c
            181-391             AL021392.5         21272-21482         c
            392-512             AL021392.5         10273-10393         c
            513-633             AL096766.12        10055-10175
            634-695             AL096766.12        13249-13310
            696-828             AL096766.12        14908-15040
            829-854             AL096766.12        15706-15731
            855-946             AL096766.12        15894-15985
            947-1038            AL096766.12        17483-17574
            1039-1087           AL096766.12        18693-18741
            1088-1159           AL096766.12        19964-20035
            1160-1203           AL096766.12        20557-20600
            1204-1313           AL096766.12        20667-20776
            1314-1468           AL096766.12        24711-24865
            1469-1613           AL096766.12        25538-25682
            1614-1707           AL096766.12        26525-26618
            1708-1792           AL096766.12        27336-27420
            1793-1861           AL096766.12        28468-28536
            1862-4500           AL096766.12        29015-31653
FEATURES             Location/Qualifiers
     source          1..4500
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="22"
                     /map="22q13.31"
     gene            1..4500
                     /gene="GRAMD4"
                     /gene_synonym="DIP"
                     /note="GRAM domain containing 4"
                     /db_xref="GeneID:23151"
                     /db_xref="HGNC:HGNC:29113"
                     /db_xref="MIM:613691"
     exon            1..180
                     /gene="GRAMD4"
                     /gene_synonym="DIP"
                     /inference="alignment:Splign:2.1.0"
     exon            181..391
                     /gene="GRAMD4"
                     /gene_synonym="DIP"
                     /inference="alignment:Splign:2.1.0"
     CDS             230..1966
                     /gene="GRAMD4"
                     /gene_synonym="DIP"
                     /note="isoform 1 is encoded by transcript variant 1; GRAM
                     domain-containing protein 4; death-inducing-protein"
                     /codon_start=1
                     /product="GRAM domain-containing protein 4 isoform 1"
                     /protein_id="NP_055939.1"
                     /db_xref="CCDS:CCDS33672.1"
                     /db_xref="GeneID:23151"
                     /db_xref="HGNC:HGNC:29113"
                     /db_xref="MIM:613691"
                     /translation="MLRRLDKIRFRGHKRDDFLDLAESPNASDTECSDEIPLKVPRTS
                     PRDSEELRDPAGPGTLIMATGVQDFNRTEFDRLNEIKGHLEIALLEKHFLQEELRKLR
                     EETNAEMLRQELDRERQRRMELEQKVQEVLKARTEEQMAQQPPKGQAQASNGAERRSQ
                     GLSSRLQKWFYERFGEYVEDFRFQPEENTVETEEPLSARRLTENMRRLKRGAKPVTNF
                     VKNLSALSDWYSVYTSAIAFTVYMNAVWHGWAIPLFLFLAILRLSLNYLIARGWRIQW
                     SIVPEVSEPVEPPKEDLTVSEKFQLVLDVAQKAQNLFGKMADILEKIKNLFMWVQPEI
                     TQKLYVALWAAFLASCFFPYRLVGLAVGLYAGIKFFLIDFIFKRCPRLRAKYDTPYII
                     WRSLPTDPQLKERSSAAVSRRLQTTSSRSYVPSAPAGLGKEEDAGRFHSTKKGNFHEI
                     FNLTENERPLAVCENGWRCCLINRDRKMPTDYIRNGVLYVTENYLCFESSKSGSSKRN
                     KVIKLVDITDIQKYKVLSVLPGSGMGIAVSTPSTQKPLVFGAMVHRDEAFETILSQYI
                     KITSAAASGGDS"
     exon            392..512
                     /gene="GRAMD4"
                     /gene_synonym="DIP"
                     /inference="alignment:Splign:2.1.0"
     exon            513..633
                     /gene="GRAMD4"
                     /gene_synonym="DIP"
                     /inference="alignment:Splign:2.1.0"
     exon            634..695
                     /gene="GRAMD4"
                     /gene_synonym="DIP"
                     /inference="alignment:Splign:2.1.0"
     exon            696..828
                     /gene="GRAMD4"
                     /gene_synonym="DIP"
                     /inference="alignment:Splign:2.1.0"
     exon            829..854
                     /gene="GRAMD4"
                     /gene_synonym="DIP"
                     /inference="alignment:Splign:2.1.0"
     exon            855..946
                     /gene="GRAMD4"
                     /gene_synonym="DIP"
                     /inference="alignment:Splign:2.1.0"
     exon            947..1038
                     /gene="GRAMD4"
                     /gene_synonym="DIP"
                     /inference="alignment:Splign:2.1.0"
     exon            1039..1087
                     /gene="GRAMD4"
                     /gene_synonym="DIP"
                     /inference="alignment:Splign:2.1.0"
     exon            1088..1159
                     /gene="GRAMD4"
                     /gene_synonym="DIP"
                     /inference="alignment:Splign:2.1.0"
     exon            1160..1203
                     /gene="GRAMD4"
                     /gene_synonym="DIP"
                     /inference="alignment:Splign:2.1.0"
     exon            1204..1313
                     /gene="GRAMD4"
                     /gene_synonym="DIP"
                     /inference="alignment:Splign:2.1.0"
     exon            1314..1468
                     /gene="GRAMD4"
                     /gene_synonym="DIP"
                     /inference="alignment:Splign:2.1.0"
     exon            1469..1613
                     /gene="GRAMD4"
                     /gene_synonym="DIP"
                     /inference="alignment:Splign:2.1.0"
     exon            1614..1707
                     /gene="GRAMD4"
                     /gene_synonym="DIP"
                     /inference="alignment:Splign:2.1.0"
     exon            1708..1792
                     /gene="GRAMD4"
                     /gene_synonym="DIP"
                     /inference="alignment:Splign:2.1.0"
     exon            1793..1861
                     /gene="GRAMD4"
                     /gene_synonym="DIP"
                     /inference="alignment:Splign:2.1.0"
     exon            1862..4500
                     /gene="GRAMD4"
                     /gene_synonym="DIP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4475..4480
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GRAMD4"
                     /gene_synonym="DIP"
                     /note="hexamer: AATAAA"
     polyA_site      4500
                     /gene="GRAMD4"
                     /gene_synonym="DIP"
                     /note="major polyA site"
ORIGIN      
        1 gcacaggact ggagtcctcc aggctccagg gcagcagaca ccaccctctc cgtgcctgct
       61 ggtgttgggc ggcttggagg ctatggttcc tgggtcctcg tagcacatcc tggttctggg
      121 ccaggacctg aaggagccac agtgaaagag tgtttctgga tgtatctgag acagacggag
      181 ggaacccgag cgtcatgtta gggtgaagca gaggacctca gtgctgaaca tgctaaggag
      241 gttggacaaa atcaggttca gaggtcacaa gagagatgac ttcctcgatc tagcggagtc
      301 tccaaatgcc tcggacaccg aatgcagcga cgaaatcccc ctgaaggtac cgcggacctc
      361 gccccgggac agcgaggagc tgagggaccc tgctggtcca gggaccctca tcatggccac
      421 aggagtccag gactttaacc ggacagagtt tgatcgactg aatgagatca aaggtcacct
      481 ggaaattgcc ttattggaaa aacatttctt acaggaggag ctccggaagc tgcgagaaga
      541 aaccaacgcg gagatgctgc ggcaggagct ggaccgcgag cggcagcggc ggatggagct
      601 ggagcagaag gtgcaggagg tgctgaaggc cagaaccgag gagcagatgg ctcagcagcc
      661 cccaaaaggg caggcccagg ccagcaatgg agcagagcgc cggagccagg ggctgtcctc
      721 gcgcctgcag aagtggttct acgagcggtt tggggagtac gtggaggact tccggttcca
      781 gcccgaggag aacactgtgg agacagagga acccctgagc gcccgcaggt taactgaaaa
      841 tatgagacgg ctcaagcgcg gtgccaagcc ggtcactaac tttgtgaaga acctctctgc
      901 cttatccgac tggtactccg tctacacgtc tgccattgcc ttcaccgtgt acatgaatgc
      961 cgtgtggcat ggctgggcca tcccattgtt cttatttcta gcaattctga ggttatccct
     1021 caattacctc atcgccaggg ggtggcggat acagtggagc atcgtgcccg aagtgtctga
     1081 gcccgtggaa cctccaaagg aagacctgac tgtgtctgag aagttccagc tggtgctgga
     1141 cgtcgcccag aaagcccaga accttttcgg gaagatggct gacatcctgg agaagatcaa
     1201 gaacttgttc atgtgggtcc agccggagat cacacagaag ctgtatgtgg cgctctgggc
     1261 tgccttcctg gcctcctgct tcttccccta ccgcctggtg gggcttgccg tgggactcta
     1321 tgctggtatc aagttcttcc tcattgattt catctttaaa cgctgcccga ggctgcgcgc
     1381 caagtacgac acgccctata tcatctggag gagtctcccc accgacccgc agctcaagga
     1441 gcgctccagc gccgcagtct cacgcaggct gcagacgacc tcgtcacgga gctacgtacc
     1501 cagcgcaccg gccggcctgg gtaaagagga ggacgccggt cgcttccaca gcaccaagaa
     1561 gggcaatttc cacgagatct tcaatctgac agaaaacgag cgtccgctgg cggtgtgcga
     1621 gaatggctgg cgctgctgcc tcatcaacag ggaccggaag atgcccacgg actacatcag
     1681 gaacggggtg ctctacgtca cggagaatta cttgtgcttc gaaagctcca aatctgggtc
     1741 ctcaaagagg aacaaagtca tcaagctagt ggacatcacg gacatccaga agtacaaggt
     1801 cctgtctgtc ctcccaggct caggcatggg gattgccgtg tcgacgccat ccacccagaa
     1861 accgctcgtg tttggtgcca tggtgcacag ggatgaggcc ttcgagacca ttctcagcca
     1921 gtacatcaag atcacctcag cggcagcgtc tggcggggac agctagtatt gacttgccca
     1981 ggacgttgct ggaattttct ttttcttttt ctttttcttt tttttttttt acgatttggt
     2041 agtggaaaca attggacatc ctcatgagct tttgcaataa ttctcctgga cctgtggttc
     2101 tattgtgttg acctctgcgt tttatcgacc aagaaggggc cagggctcac agggacgggg
     2161 gtgcccctct cccacagggc acgtcaggtg cctctgaggg ccacccgcag actgggggag
     2221 ggggcagagg ccctcggggg cccgtggaga agacacacag gacccctggc cctgcccttc
     2281 tccgttccag cctggacaga gaaacctctc cagccacccc aagaggttct cgcaaccttg
     2341 tgtcccgctc tccagaggcc agaagctcgt ccaccaccaa agccatagct gaagagtgcg
     2401 gggcccttcc tcctggggac agaaagatgt cgtcaaggag ggacatgggg gcctttcacc
     2461 aaccaccgag aaacgggcct ggcggccctc cttcctctta catgagaccc tcctgtggca
     2521 tttgcccttg gtgccgggct ggggccgggc gcagtgaccc tgcctgcgct ccacactcgc
     2581 tccacgggaa cagagagggt gagaagggcc cacccctcgc ctgccctcag tgtctttggt
     2641 ggcaccttcc ttgctggcct ccagggcgct cagcaccgcg tctgtaaggg cctgcctgct
     2701 gctctcggcc tgacacgccg gccaggaggt ctgtagctgg ggaccagtaa gggcacagga
     2761 tggtgcaggt aaaagcacat ctttctcaca ctttgctctt tggaaggccc aggagaacat
     2821 ccgcgaaggc tgttggaggt gctccgagca ctgtggcatg tctggcacat ggcccccagg
     2881 ctgcggttgc ctgggttggt tgggggagga agtggggagg agtgttccgg gaccatggtg
     2941 gcccaggctg cagccgcctt tgggccatcc gagaggctct ggcagcccct gtgctttagg
     3001 gagcaaccgt gagccgagcc cagaggcctg ggcctgcact gcctgcagcc gacatgcgac
     3061 agcgttccct cccccgcgtg cctagccggt gccggtccgg gcacagaccc ccccagcccc
     3121 cgccctgccc cagggaagcc tgggcttccc gggaacaagg tggcatttgt ggagggagcg
     3181 cccgcaggcc tggtctgctg gggccgcctg cgctgggctg aagggaggga aaggcggctt
     3241 gggcctcctg gaaggaggtg gccaccccgc gggcctgcgt gtctgctggg gcggatcccg
     3301 cagctccctc agcttgtcct gagtcccttg ggtgtcgttg agattgttgt tttttgaaga
     3361 aacagaagat tctatttttt acagcgagca agctggtttt cttatttttg tatccttttt
     3421 cagatgtaat ttttatcttt gctccgatcc tcatttgctg gtgtgggtga gggatccggc
     3481 ggcatgggct ggtttcaccc ccttcacgag gggccgcaga gtcacacgct ggtgccgggg
     3541 gtgctttggg gggagctgcg ccgatcacca gattaagcac atgtcctatc ccaggcggtg
     3601 gagcggagcc cccgtggctc tggactgcgc ggacgttggc gtcaggatga ccacacggcg
     3661 gcctttcccg aatggggaca gaacccgctc tgagccgtgg gtctggctcc tgtaggggac
     3721 tggctctctt ggtgcaccag gggaggggga catatcccag tgaaccccac cttggcgcct
     3781 gaggcaacac agggtgggca ctgacccacc cccaggggcg gctgcagagg cagtgcccgc
     3841 agacaatggc cacacctctc tccccagggc ccggcagtgc ccaaggatgg gtccggggcc
     3901 tcggggccaa tgagcgcctc ttcctaggtg ctgggattca gtccccaaac acagcgggag
     3961 gggtccctgg ggcagatggg gctttaccag cgtcgggtgg tttagttcga gtcccttttg
     4021 tggagaaagg gagatgaaaa ctgaccacgt gccaggtgtg gccgaagccc ccagggaggg
     4081 ccacattcgg ggagcggggg gtcgggggag ggccaccgac tggctctgct gccagcacag
     4141 gcccctccct ggaagtcctc gggagcggag cgcggatcgg cacgggctct gggctccccg
     4201 tggagagaag ctgtagtttt taccaaattg tgtacatctg ggcagatgtt taatttctgt
     4261 gactaatcac tgaactagac gaatgttaaa ttttttatgt ctgaagcctg agtctatttt
     4321 ggatctgtaa ataatcattg ccagtgtgac ttttgttcaa caaaaggatt gtactgtatt
     4381 aagaaccgat gaaaaaaatt ctcctgtaac atttttttaa gaaaactttg tttgtttaaa
     4441 gaaaaagtat tgtataaatt ataattttta tttaaataaa cctaaaatgc tttgtgctaa
//
