LOCUS       NM_001012642            3284 bp    mRNA    linear   PRI 01-JUN-2024
DEFINITION  Homo sapiens GRAM domain containing 2A (GRAMD2A), mRNA.
ACCESSION   NM_001012642 XM_113796
VERSION     NM_001012642.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3284)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   2  (bases 1 to 3284)
  AUTHORS   Besprozvannaya,M., Dickson,E., Li,H., Ginburg,K.S., Bers,D.M.,
            Auwerx,J. and Nunnari,J.
  TITLE     GRAM domain proteins specialize functionally distinct ER-PM contact
            sites in human cells
  JOURNAL   Elife 7, e31019 (2018)
   PUBMED   29469807
  REMARK    GeneRIF: GRAMD2a, but not GRAMD1a, co-localizes with the E-Syt2/3
            tethers at endoplasmic reticulum-plasma membrane contact sites in a
            phosphatidylinositol phosphate lipid-dependent manner. Data from an
            analysis of cells lacking GRAMD2a suggest that it is an organizer
            of the membrane contact sites with pleiotropic functions including
            calcium homeostasis.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3284)
  AUTHORS   Luykx,J.J., Bakker,S.C., Lentjes,E., Neeleman,M., Strengman,E.,
            Mentink,L., DeYoung,J., de Jong,S., Sul,J.H., Eskin,E., van
            Eijk,K., van Setten,J., Buizer-Voskamp,J.E., Cantor,R.M., Lu,A.,
            van Amerongen,M., van Dongen,E.P., Keijzers,P., Kappen,T.,
            Borgdorff,P., Bruins,P., Derks,E.M., Kahn,R.S. and Ophoff,R.A.
  TITLE     Genome-wide association study of monoamine metabolite levels in
            human cerebrospinal fluid
  JOURNAL   Mol Psychiatry 19 (2), 228-234 (2014)
   PUBMED   23319000
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DA854662.1, BC038451.1 and BM663624.1.
            
            On Nov 22, 2018 this sequence version replaced NM_001012642.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC038451.1, AK095072.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000309731.12/ ENSP00000311657.7
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-18                DA854662.1         9-26
            19-2754             BC038451.1         1-2736
            2755-2756           BM663624.1         16-17
            2757-3284           BC038451.1         2737-3264
FEATURES             Location/Qualifiers
     source          1..3284
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="15"
                     /map="15q23"
     gene            1..3284
                     /gene="GRAMD2A"
                     /gene_synonym="GRAMD2"
                     /note="GRAM domain containing 2A"
                     /db_xref="GeneID:196996"
                     /db_xref="HGNC:HGNC:27287"
                     /db_xref="MIM:620181"
     exon            1..57
                     /gene="GRAMD2A"
                     /gene_synonym="GRAMD2"
                     /inference="alignment:Splign:2.1.0"
     CDS             17..1081
                     /gene="GRAMD2A"
                     /gene_synonym="GRAMD2"
                     /note="GRAM domain-containing protein 2; GRAM domain
                     containing 2"
                     /codon_start=1
                     /product="GRAM domain-containing protein 2A"
                     /protein_id="NP_001012660.1"
                     /db_xref="CCDS:CCDS32283.1"
                     /db_xref="GeneID:196996"
                     /db_xref="HGNC:HGNC:27287"
                     /db_xref="MIM:620181"
                     /translation="MTALSRSEATEEGGNQQMHRKTASLNSPVSCKEKPDRVEEPPDY
                     SLHWPEGLKGEEIKKCGREGITLNKYNQQYHKLFKDVPLEEVVLKVCSCALQRDFLLQ
                     GRLYISPNWLCFHASLFGKDIKVVIPVVSVQMIKKHKMARLLPNGLAITTNTSQKYIF
                     VSLLSRDSVYDLLRRVCTHLQPSSKKSLSVREFSGEPESLEVLIPEMKWRKVCPSSRS
                     LSLPDNIPCIPPSSVDSTDSFFPSRKPPMSEKSRAQVASENGGRWAWPMPGWGPACPK
                     KMPNCSPTAKNAVYEEDELEEEPRSTGELRLWDYRLLKVFFVLICFLVMSSSYLAFRI
                     SRLEQQLCSLSWDDPVPGHR"
     exon            58..150
                     /gene="GRAMD2A"
                     /gene_synonym="GRAMD2"
                     /inference="alignment:Splign:2.1.0"
     exon            151..208
                     /gene="GRAMD2A"
                     /gene_synonym="GRAMD2"
                     /inference="alignment:Splign:2.1.0"
     exon            209..284
                     /gene="GRAMD2A"
                     /gene_synonym="GRAMD2"
                     /inference="alignment:Splign:2.1.0"
     exon            285..388
                     /gene="GRAMD2A"
                     /gene_synonym="GRAMD2"
                     /inference="alignment:Splign:2.1.0"
     exon            389..487
                     /gene="GRAMD2A"
                     /gene_synonym="GRAMD2"
                     /inference="alignment:Splign:2.1.0"
     exon            488..559
                     /gene="GRAMD2A"
                     /gene_synonym="GRAMD2"
                     /inference="alignment:Splign:2.1.0"
     exon            560..616
                     /gene="GRAMD2A"
                     /gene_synonym="GRAMD2"
                     /inference="alignment:Splign:2.1.0"
     exon            617..761
                     /gene="GRAMD2A"
                     /gene_synonym="GRAMD2"
                     /inference="alignment:Splign:2.1.0"
     exon            762..972
                     /gene="GRAMD2A"
                     /gene_synonym="GRAMD2"
                     /inference="alignment:Splign:2.1.0"
     exon            973..1077
                     /gene="GRAMD2A"
                     /gene_synonym="GRAMD2"
                     /inference="alignment:Splign:2.1.0"
     exon            1078..3284
                     /gene="GRAMD2A"
                     /gene_synonym="GRAMD2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3258..3263
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GRAMD2A"
                     /gene_synonym="GRAMD2"
                     /note="hexamer: AATAAA"
     polyA_site      3284
                     /gene="GRAMD2A"
                     /gene_synonym="GRAMD2"
                     /note="major polyA site"
ORIGIN      
        1 gggtgcaggc gccgggatga ccgctttaag ccggagcgag gccaccgagg agggcggcaa
       61 ccaacaaatg cacagaaaga cagcttctct gaacagtcct gtgtcctgca aagagaaacc
      121 agacagagtt gaggagcccc cggactacag tctgcactgg ccagaaggct tgaagggtga
      181 agagataaag aagtgtggcc gagaagggat aacactgaat aaatacaacc agcaatacca
      241 caagctgttt aaggatgttc ccttggagga agtggttctc aaagtgtgtt cctgtgccct
      301 ccagagggac ttcctcctcc agggccggct ctacatctcc cccaactggc tctgcttcca
      361 tgccagcctc tttggcaagg atatcaaggt ggtcattcct gtggtgtctg tgcaaatgat
      421 caaaaaacac aagatggcac ggctccttcc caatggactg gccatcacca ccaacaccag
      481 ccagaagtat atctttgtgt cactgctctc ccgggacagt gtatatgacc tgctgaggag
      541 agtctgcacc cacctacagc cttccagcaa gaagagtctg agtgtaagag aattttcagg
      601 ggaacctgag tctctggaag tcctcatccc tgagatgaag tggagaaagg tatgcccttc
      661 ctccaggtcc ctgtctctcc cagacaacat cccttgtatc cctccatcat ccgtggactc
      721 cacagacagt ttcttcccct ccaggaagcc tccaatgtct gaaaagtcaa gagcccaagt
      781 agcttcagaa aatggtggga ggtgggcatg gcccatgcca ggctggggtc ctgcctgccc
      841 taagaagatg ccgaactgct ctcccactgc aaagaatgct gtctatgagg aggatgagct
      901 ggaggaggag cccaggagca ctggggagct gaggctctgg gattaccggc tcctcaaggt
      961 cttctttgtg ctgatctgct tcctggtcat gtcctcatcc tacctggcgt tccgtatttc
     1021 tcggctagag cagcagttat gctccttgag ttgggatgac ccagtccctg ggcacaggta
     1081 acagccactt ggcctttgtc cccattcctt ctccaagaag aatgttctgg gtgctaagtc
     1141 tccaccaatg atccctgtgg tttatgctgc tgctgtgcta agactgccta tgaaggaaaa
     1201 tattccagat ccctcctcct ccccgcagct cttctcttcc ctctatgaga gatctgaagt
     1261 acgtgtttac aaagtaactg gcttctacaa tttttggact caagcaaaaa gccagatttt
     1321 ttggaaccaa ctgaggaatt ctgtacacaa agcttcagca aacacttaga gaaaacaaac
     1381 aaacaaacaa actgggtcct tggaaacagt tctggcacac aggcagaaca ctggaggcca
     1441 cctcaaagga cagaggccat gctttcgcat ttggaatgtg gctctgccac acagctcact
     1501 cctatggtgg ggtcagaggt cagaacacaa accacccatg tgggctctgc tccctcccaa
     1561 cactctgtgt tcccacaggg cttctctgcg ggcagccagg cacctgtcag gcttgggcag
     1621 gggcctgctg agaatctgtg aatgcagagg ggagggctca tggccaggat tccagccaga
     1681 gatgaatttc catgctctgg gccagggctc tgtgccggcc cctcatgagg tggctggctg
     1741 cattatgggg agatgctgcc cacggcggag atggtggcag tggctgtgaa gaacagaggt
     1801 acctgaattc tgtgtcctca gctgtcactt tccggtgaga caagtggagc agggcctttg
     1861 atcactgtgg tcacagacct ggtactgggt tttgcccttg tgtgctcaaa catttctaag
     1921 gtgtcccaag ccctggaaat agggaaggga agggtggagg cttggatctg aggtcttgct
     1981 gtccactccc tgcctgcctg cccgcatctc aggcatggcc aacagcagcc cgttgagggg
     2041 ggcttgtctg gacagttgcg tgtgatggtg ttgagccatc acagggcaga ggtcacttgt
     2101 gtcaccccca tagtcattgg gttgcaggcc atggaggagg gacctccact ccatggggcc
     2161 tattctccat taaggtggaa gtgtccactg tgggcctagc acagtgcagg aaccaggcca
     2221 gggggtctat ctcatacctc tggggacagg aaaggtagag gatgaggagg ttggacgtgg
     2281 tggtggcttt gccttggata ttcctcttcc tctatcaagg ccttctcttc ttctctgtgt
     2341 cccacaactc tggcctcatc gccctgggac tcccatctgc taagatagtg gctttggagg
     2401 ttctggaagg gcttagtgag gaggccagaa gtatcaggga aagttgctgg tgatggggtg
     2461 ggcggtgtca gaagcagcca ctgttttccc ctcttcagac actgagagat taatcaggga
     2521 atgaaatcca ggtggaaatt atttattgtg aagcagacga ctgtctgtcc tgtttatgac
     2581 aaacccaaat gagaattgga gggactacca gtaagtggtc tcttcatgct ctccccatcc
     2641 cagtttttag gatgaaattt agagcctggc cttttgtgtt gcttgcacca aatctgtatg
     2701 tctcaggtga aactcactgt tccatgaatg gtcatccttt tttttttttt ttttttggaa
     2761 ccagcccctt tcaccctttc atccgtcttt tttttttagg aaaataaccc ttcttgcttc
     2821 agcacttctc cctggtcctt gctgaaagac agagataaaa atccattctc tgagccttgc
     2881 ttggttgtca gaggccatga gaggtgccag ttataggtgg atgtgccaag atgctggtga
     2941 acttggtctt cagctatacc caggctcaga aagggcaaga gccatgctgc agcgtaggtg
     3001 actttggagg tgcacttggg gcccagggct ttgagtgttg cgggtgtgcc tgtccctcca
     3061 gatagtgctc tgtttctctc tgttgtcccc ctgcctggtc ctctggggcc actgtgcttt
     3121 ctgctgtgtg catttataaa tgatgtgtat tttatataga cctgcttgca ttggctgatg
     3181 ctcctctaat tccctgagtt tgattcaacc acccttgggt tgttttgcta tggccttagc
     3241 ctttgaaaat attttaaaat aaaaaacttg atttttctag tgta
//
