LOCUS       NM_020895               2659 bp    mRNA    linear   PRI 06-APR-2024
DEFINITION  Homo sapiens GRAM domain containing 1A (GRAMD1A), transcript
            variant 1, mRNA.
ACCESSION   NM_020895
VERSION     NM_020895.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2659)
  AUTHORS   Liu,Y., Fu,S., Zhang,Z., Wang,S., Cheng,X., Li,Z., Ding,Y., Sun,T.
            and Ma,M.
  TITLE     GRAMD1A Is a Biomarker of Kidney Renal Clear Cell Carcinoma and Is
            Associated with Immune Infiltration in the Tumour Microenvironment
  JOURNAL   Dis Markers 2022, 5939021 (2022)
   PUBMED   35860689
  REMARK    GeneRIF: GRAMD1A Is a Biomarker of Kidney Renal Clear Cell
            Carcinoma and Is Associated with Immune Infiltration in the Tumour
            Microenvironment.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2659)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 2659)
  AUTHORS   Besprozvannaya,M., Dickson,E., Li,H., Ginburg,K.S., Bers,D.M.,
            Auwerx,J. and Nunnari,J.
  TITLE     GRAM domain proteins specialize functionally distinct ER-PM contact
            sites in human cells
  JOURNAL   Elife 7, e31019 (2018)
   PUBMED   29469807
  REMARK    GeneRIF: GRAMD2a, but not GRAMD1a, co-localizes with the E-Syt2/3
            tethers at endoplasmic reticulum-plasma membrane contact sites in a
            phosphatidylinositol phosphate lipid-dependent manner. Data from an
            analysis of cells lacking GRAMD2a suggest that it is an organizer
            of the membrane contact sites with pleiotropic functions including
            calcium homeostasis.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2659)
  AUTHORS   Fu,B., Meng,W., Zhao,H., Zhang,B., Tang,H., Zou,Y., Yao,J., Li,H.
            and Zhang,T.
  TITLE     GRAM domain-containing protein 1A (GRAMD1A) promotes the expansion
            of hepatocellular carcinoma stem cell and hepatocellular carcinoma
            growth through STAT5
  JOURNAL   Sci Rep 6, 31963 (2016)
   PUBMED   27585821
  REMARK    GeneRIF: STAT5 was the target of GRAMD1A; GRAMD1A regulated the
            target genes of STAT5 and the transcriptional activity of STAT5.
            Inhibition of STAT5 in indicated HCC cells overexpressing GRAMD1A
            suppressed the effects of GRAMD1A on the self-renewal of HCC stem
            cell, resistance to chemotherapy and tumor growth, suggesting
            GRAMD1A promoted the self-renewal of HCC stem cells and the
            development of HCC by increasing STAT5 level.
            Publication Status: Online-Only
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DA390646.1, BC014077.2, AB040966.1 and CD367242.1.
            
            On Jun 2, 2019 this sequence version replaced NM_020895.4.
            
            Transcript Variant: This variant (1) encodes isoform 1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC014077.2, SRR1660809.70291.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1966682, SAMEA1968540
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000317991.10/ ENSP00000441032.1
            RefSeq Select criteria :: based on manual assertion, conservation
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-127               DA390646.1         4-130
            128-1226            BC014077.2         119-1217
            1227-1377           AB040966.1         861-1011
            1378-2084           BC014077.2         1369-2075
            2085-2659           CD367242.1         21-595              c
FEATURES             Location/Qualifiers
     source          1..2659
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="19"
                     /map="19q13.11"
     gene            1..2659
                     /gene="GRAMD1A"
                     /gene_synonym="KIAA1533"
                     /note="GRAM domain containing 1A"
                     /db_xref="GeneID:57655"
                     /db_xref="HGNC:HGNC:29305"
                     /db_xref="MIM:620178"
     exon            1..164
                     /gene="GRAMD1A"
                     /gene_synonym="KIAA1533"
                     /inference="alignment:Splign:2.1.0"
     CDS             157..2331
                     /gene="GRAMD1A"
                     /gene_synonym="KIAA1533"
                     /note="isoform 1 is encoded by transcript variant 1; GRAM
                     domain-containing protein 1A; protein Aster-A"
                     /codon_start=1
                     /product="protein Aster-A isoform 1"
                     /protein_id="NP_065946.2"
                     /db_xref="CCDS:CCDS42546.1"
                     /db_xref="GeneID:57655"
                     /db_xref="HGNC:HGNC:29305"
                     /db_xref="MIM:620178"
                     /translation="MFDTTPHSGRSTPSSSPSLRKRLQLLPPSRPPPEPEPGTMVEKG
                     SDSSSEKGGVPGTPSTQSLGSRNFIRNSKKMQSWYSMLSPTYKQRNEDFRKLFSKLPE
                     AERLIVDYSCALQREILLQGRLYLSENWICFYSNIFRWETTISIQLKEVTCLKKEKTA
                     KLIPNAIQICTESEKHFFTSFGARDRCFLLIFRLWQNALLEKTLSPRELWHLVHQCYG
                     SELGLTSEDEDYVSPLQLNGLGTPKEVGDVIALSDITSSGAADRSQEPSPVGSRRGHV
                     TPNLSRASSDADHGAEEDKEEQVDSQPDASSSQTVTPVAEPPSTEPTQPDGPTTLGPL
                     DLLPSEELLTDTSNSSSSTGEEADLAALLPDLSGRLLINSVFHVGAERLQQMLFSDSP
                     FLQGFLQQCKFTDVTLSPWSGDSKCHQRRVLTYTIPISNPLGPKSASVVETQTLFRRG
                     PQAGGCVVDSEVLTQGIPYQDYFYTAHRYCILGLARNKARLRVSSEIRYRKQPWSLVK
                     SLIEKNSWSGIEDYFHHLERELAKAEKLSLEEGGKDARGLLSGLRRRKRPLSWRAHGD
                     GPQHPDPDPCARAGIHTSGSLSSRFSEPSVDQGPGAGIPSALVLISIVICVSLIILIA
                     LNVLLFYRLWSLERTAHTFESWHSLALAKGKFPQTATEWAEILALQKQFHSVEVHKWR
                     QILRASVELLDEMKFSLEKLHQGITVSDPPFDTQPRPDDSFS"
     exon            165..375
                     /gene="GRAMD1A"
                     /gene_synonym="KIAA1533"
                     /inference="alignment:Splign:2.1.0"
     exon            376..396
                     /gene="GRAMD1A"
                     /gene_synonym="KIAA1533"
                     /inference="alignment:Splign:2.1.0"
     exon            397..481
                     /gene="GRAMD1A"
                     /gene_synonym="KIAA1533"
                     /inference="alignment:Splign:2.1.0"
     exon            482..585
                     /gene="GRAMD1A"
                     /gene_synonym="KIAA1533"
                     /inference="alignment:Splign:2.1.0"
     exon            586..681
                     /gene="GRAMD1A"
                     /gene_synonym="KIAA1533"
                     /inference="alignment:Splign:2.1.0"
     exon            682..762
                     /gene="GRAMD1A"
                     /gene_synonym="KIAA1533"
                     /inference="alignment:Splign:2.1.0"
     exon            763..875
                     /gene="GRAMD1A"
                     /gene_synonym="KIAA1533"
                     /inference="alignment:Splign:2.1.0"
     exon            876..1026
                     /gene="GRAMD1A"
                     /gene_synonym="KIAA1533"
                     /inference="alignment:Splign:2.1.0"
     exon            1027..1225
                     /gene="GRAMD1A"
                     /gene_synonym="KIAA1533"
                     /inference="alignment:Splign:2.1.0"
     exon            1226..1369
                     /gene="GRAMD1A"
                     /gene_synonym="KIAA1533"
                     /inference="alignment:Splign:2.1.0"
     exon            1370..1488
                     /gene="GRAMD1A"
                     /gene_synonym="KIAA1533"
                     /inference="alignment:Splign:2.1.0"
     exon            1489..1631
                     /gene="GRAMD1A"
                     /gene_synonym="KIAA1533"
                     /inference="alignment:Splign:2.1.0"
     exon            1632..1735
                     /gene="GRAMD1A"
                     /gene_synonym="KIAA1533"
                     /inference="alignment:Splign:2.1.0"
     exon            1736..1909
                     /gene="GRAMD1A"
                     /gene_synonym="KIAA1533"
                     /inference="alignment:Splign:2.1.0"
     exon            1910..1997
                     /gene="GRAMD1A"
                     /gene_synonym="KIAA1533"
                     /inference="alignment:Splign:2.1.0"
     exon            1998..2009
                     /gene="GRAMD1A"
                     /gene_synonym="KIAA1533"
                     /inference="alignment:Splign:2.1.0"
     exon            2010..2117
                     /gene="GRAMD1A"
                     /gene_synonym="KIAA1533"
                     /inference="alignment:Splign:2.1.0"
     exon            2118..2238
                     /gene="GRAMD1A"
                     /gene_synonym="KIAA1533"
                     /inference="alignment:Splign:2.1.0"
     exon            2239..2659
                     /gene="GRAMD1A"
                     /gene_synonym="KIAA1533"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2642..2647
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GRAMD1A"
                     /gene_synonym="KIAA1533"
                     /note="hexamer: AATAAA"
     polyA_site      2659
                     /gene="GRAMD1A"
                     /gene_synonym="KIAA1533"
                     /note="major polyA site"
ORIGIN      
        1 cttttgtgcg ggcggttggg tcgggtgggg gcggcggccg cggaaggcca ggccggtgcc
       61 ctgcggggac gcccagcgca gcccagcccc gcgcagccca gccctgccct gccctgccct
      121 gccctgcgcc cggggcgcgc ccaccgcgcc gcatccatgt tcgacaccac accccactct
      181 ggccggagca cgccaagcag ctccccatcg ctccggaaac ggctgcagct cctgccccca
      241 agccggcccc cacctgagcc agaaccaggc accatggtgg agaagggatc agatagctcc
      301 tcagagaagg gtggggtgcc tgggaccccc agcacccaga gcctaggcag ccggaacttc
      361 atccgcaaca gcaagaagat gcagagctgg tacagtatgc tgagccccac ttataagcag
      421 cgtaatgagg acttccggaa actgttcagc aaactccccg aagcagaacg cctcattgtg
      481 gattactcct gcgccctgca gcgtgagatc ctgctccagg gccgcctcta cctctctgag
      541 aactggatct gcttctacag caacatcttc cgctgggaga ccacgatctc catccagctg
      601 aaggaagtga catgtctgaa gaaggaaaag acggccaagc tgatccccaa cgccatccag
      661 atctgcacgg agagcgagaa gcatttcttc acttcctttg gggcccgtga ccgctgcttc
      721 ctcctcatct tccgcctctg gcagaatgca ctgcttgaaa agacgctgag tccccgcgag
      781 ctctggcacc tggtgcatca gtgctacggc tcagagctgg gcctcaccag tgaggatgag
      841 gactatgtct cccccttgca gctgaacggt ctggggaccc ccaaggaagt gggagatgtg
      901 atcgccctga gcgacatcac ctcctcgggg gcagctgacc gcagccagga gccaagccca
      961 gtgggttcgc gccgtggcca tgtcacgccc aacctttccc gagccagcag cgacgcagac
     1021 catggggcag aggaggacaa ggaggagcag gtagacagcc agccagacgc ctcctccagc
     1081 cagacagtga ccccggtggc tgaacccccg agcacagagc ccacccagcc tgacgggccc
     1141 accaccctgg gccccttgga tctgctgccc agtgaggagc tattgacaga cacaagtaac
     1201 tcctcttcat ccactgggga ggaagcggac ttggctgccc tgcttcccga cctctccggc
     1261 cgcctcctca tcaactctgt cttccatgtg ggcgctgagc ggctccagca gatgctcttc
     1321 tcggactcgc ccttcctcca gggcttccta cagcagtgca agttcacaga cgtgacgctg
     1381 agcccctgga gtggggacag caagtgccac cagcgccggg tgctgacgta caccatcccc
     1441 atcagcaacc cactgggccc caagagcgcc tccgtggtgg agacacagac gctgttccgg
     1501 cgcggccccc aggccggcgg gtgtgtggtg gactccgagg tgctgacgca gggcatcccc
     1561 taccaggact acttctacac tgcccaccgc tactgcatcc tgggtctggc ccggaacaag
     1621 gcgcggctcc gagtgtcttc tgagatccgc taccgaaagc agccgtggag cctggtgaag
     1681 tcgctcattg agaagaactc gtggagcggc attgaagact atttccacca tctggagcga
     1741 gagctcgcca aggctgagaa gctgtctctg gaggaaggcg ggaaggatgc ccggggcttg
     1801 ctatccggcc tgcggcggcg gaagcggccc ctgagctggc gggctcacgg ggacgggccc
     1861 cagcacccag atcctgaccc ctgtgcccgg gccggcattc acacctcggg ctccctcagc
     1921 tcccgcttct ccgaaccatc tgtggaccag ggccccgggg caggcatccc cagtgccctg
     1981 gttctcatca gcattgtgat ctgtgtgagc cttatcatcc tcatcgccct caacgtcctg
     2041 ctcttctacc gcctctggtc cctggaaagg acagcccaca cctttgagtc ctggcacagc
     2101 ctggccctgg ccaagggcaa gttcccccag acggccacag agtgggccga gatcctggcg
     2161 ctgcagaagc aattccacag cgtggaggtg cacaagtgga ggcagatcct gcgggcctcc
     2221 gtggagctcc tggatgagat gaagttctcg ctggagaagc tgcaccaagg catcacagtc
     2281 tcagaccctc cctttgacac ccagccccgg cccgatgaca gcttttcctg aggaccccgg
     2341 ccacgcagct gttcccccac atggacagat ggacacacag agcctcggcg gccactgctg
     2401 gcacggtgtg agcgccaggc atctcccacc cgcccctccc gacggcccaa ccaggggctg
     2461 tgcagacgtg gggaccacgg aaccgagatg cactttagac cagggagctg gcccggcctc
     2521 tggcaggccc cccactaact tattttgccc ggctgaggtt gtggggggcg cctcctgggg
     2581 tgcacgattc cctcagctct gggtttaatg tattatattt atttggggcc gacagtgccc
     2641 caataaaggg tcagaagtg
//
