LOCUS       NM_013296               7152 bp    mRNA    linear   PRI 06-DEC-2024
DEFINITION  Homo sapiens G protein signaling modulator 2 (GPSM2), transcript
            variant 3, mRNA.
ACCESSION   NM_013296
VERSION     NM_013296.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7152)
  AUTHORS   Kadhim,D.J., Azari,H., Sokhangouy,S.K., Hassanian,S.M.,
            Alshekarchi,H.I., Goshayeshi,L., Goshayeshi,L., Abbaszadegan,M.R.,
            Khojasteh-Leylakoohi,F., Khazaei,M., Gataa,I.S., Peters,G.J., A
            Ferns,G., Batra,J., Lam,A.K., Giovannetti,E. and Avan,A.
  TITLE     G-Protein Signaling Modulator 2 as a Potential Biomarker in
            Colorectal Cancer: Integrative Analysis Using Genetic Profiling and
            Pan-Cancer Studies
  JOURNAL   Genes (Basel) 15 (4), 474 (2024)
   PUBMED   38674408
  REMARK    GeneRIF: G-Protein Signaling Modulator 2 as a Potential Biomarker
            in Colorectal Cancer: Integrative Analysis Using Genetic Profiling
            and Pan-Cancer Studies.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7152)
  AUTHORS   Esser,R.L., Bruck,C., Thiele,J., Golumba-Nagy,V., Meyer,A.,
            Steinbach-Knodgen,E., Yan,S., Tho Pesch,C., Stahl,D., Schiller,J.
            and Kofler,D.M.
  TITLE     Truncated isoforms of GPSM2 containing the GoLoco motif region
            promote CD4+ T-cell migration in SLE
  JOURNAL   Lupus Sci Med 9 (1) (2022)
   PUBMED   35940821
  REMARK    GeneRIF: Truncated isoforms of GPSM2 containing the GoLoco motif
            region promote CD4(+) T-cell migration in SLE.
REFERENCE   3  (bases 1 to 7152)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   4  (bases 1 to 7152)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   5  (bases 1 to 7152)
  AUTHORS   Yang,D., Ji,F., Li,Y., Jiao,Y. and Fang,X.
  TITLE     GPSM2 Serves as an Independent Prognostic Biomarker for Liver
            Cancer Survival
  JOURNAL   Technol Cancer Res Treat 19, 1533033820945817 (2020)
   PUBMED   32812493
  REMARK    GeneRIF: GPSM2 Serves as an Independent Prognostic Biomarker for
            Liver Cancer Survival.
REFERENCE   6  (bases 1 to 7152)
  AUTHORS   Du,Q., Taylor,L., Compton,D.A. and Macara,I.G.
  TITLE     LGN blocks the ability of NuMA to bind and stabilize microtubules.
            A mechanism for mitotic spindle assembly regulation
  JOURNAL   Curr Biol 12 (22), 1928-1933 (2002)
   PUBMED   12445386
REFERENCE   7  (bases 1 to 7152)
  AUTHORS   Blumer,J.B., Chandler,L.J. and Lanier,S.M.
  TITLE     Expression analysis and subcellular distribution of the two
            G-protein regulators AGS3 and LGN indicate distinct functionality.
            Localization of LGN to the midbody during cytokinesis
  JOURNAL   J Biol Chem 277 (18), 15897-15903 (2002)
   PUBMED   11832491
  REMARK    GeneRIF: LGN is expressed in neuronal, astroglial, and microglial
            cultures
REFERENCE   8  (bases 1 to 7152)
  AUTHORS   Du,Q., Stukenberg,P.T. and Macara,I.G.
  TITLE     A mammalian Partner of inscuteable binds NuMA and regulates mitotic
            spindle organization
  JOURNAL   Nat Cell Biol 3 (12), 1069-1075 (2001)
   PUBMED   11781568
REFERENCE   9  (bases 1 to 7152)
  AUTHORS   Mochizuki,N., Cho,G., Wen,B. and Insel,P.A.
  TITLE     Identification and cDNA cloning of a novel human mosaic protein,
            LGN, based on interaction with G alpha i2
  JOURNAL   Gene 181 (1-2), 39-43 (1996)
   PUBMED   8973305
REFERENCE   10 (bases 1 to 7152)
  AUTHORS   Shearer,A.E., Hildebrand,M.S., Odell,A.M. and Smith,R.J.H.
  TITLE     Genetic Hearing Loss Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301607
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DC341689.1, BC027732.2,
            AI224972.1 and AL449266.17.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_013296.4.
            
            Summary: The protein encoded by this gene belongs to a family of
            proteins that modulate activation of G proteins, which transduce
            extracellular signals received by cell surface receptors into
            integrated cellular responses. The N-terminal half of this protein
            contains 10 copies of leu-gly-asn (LGN) repeat, and the C-terminal
            half contains 4 GoLoco motifs, which are involved in guanine
            nucleotide exchange. This protein may play a role in neuroblast
            division and in the development of normal hearing. Mutations in
            this gene are associated with autosomal recessive nonsyndromic
            deafness (DFNB82). Alternative splicing results in multiple
            transcript variants. [provided by RefSeq, Mar 2016].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803612.14773.1,
                                           SRR1803615.188004.1 [ECO:0000332]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000264126.9/ ENSP00000264126.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-220               DC341689.1         5-224
            221-2912            BC027732.2         1-2692
            2913-3029           AI224972.1         7-123               c
            3030-7152           AL449266.17        139297-143419
FEATURES             Location/Qualifiers
     source          1..7152
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p13.3"
     gene            1..7152
                     /gene="GPSM2"
                     /gene_synonym="CMCS; DFNB82; LGN; PINS"
                     /note="G protein signaling modulator 2"
                     /db_xref="GeneID:29899"
                     /db_xref="HGNC:HGNC:29501"
                     /db_xref="MIM:609245"
     exon            1..244
                     /gene="GPSM2"
                     /gene_synonym="CMCS; DFNB82; LGN; PINS"
                     /inference="alignment:Splign:2.1.0"
     exon            245..548
                     /gene="GPSM2"
                     /gene_synonym="CMCS; DFNB82; LGN; PINS"
                     /inference="alignment:Splign:2.1.0"
     CDS             493..2547
                     /gene="GPSM2"
                     /gene_synonym="CMCS; DFNB82; LGN; PINS"
                     /note="G-protein signalling modulator 2 (AGS3-like, C.
                     elegans); mosaic protein LGN"
                     /codon_start=1
                     /product="G-protein-signaling modulator 2"
                     /protein_id="NP_037428.3"
                     /db_xref="CCDS:CCDS792.2"
                     /db_xref="GeneID:29899"
                     /db_xref="HGNC:HGNC:29501"
                     /db_xref="MIM:609245"
                     /translation="MEENLISMREDHSFHVRYRMEASCLELALEGERLCKSGDCRAGV
                     SFFEAAVQVGTEDLKTLSAIYSQLGNAYFYLHDYAKALEYHHHDLTLARTIGDQLGEA
                     KASGNLGNTLKVLGNFDEAIVCCQRHLDISRELNDKVGEARALYNLGNVYHAKGKSFG
                     CPGPQDVGEFPEEVRDALQAAVDFYEENLSLVTALGDRAAQGRAFGNLGNTHYLLGNF
                     RDAVIAHEQRLLIAKEFGDKAAERRAYSNLGNAYIFLGEFETASEYYKKTLLLARQLK
                     DRAVEAQSCYSLGNTYTLLQDYEKAIDYHLKHLAIAQELNDRIGEGRACWSLGNAYTA
                     LGNHDQAMHFAEKHLEISREVGDKSGELTARLNLSDLQMVLGLSYSTNNSIMSENTEI
                     DSSLNGVRPKLGRRHSMENMELMKLTPEKVQNWNSEILAKQKPLIAKPSAKLLFVNRL
                     KGKKYKTNSSTKVLQDASNSIDHRIPNSQRKISADTIGDEGFFDLLSRFQSNRMDDQR
                     CCLQEKNCHTASTTTSSTPPKMMLKTSSVPVVSPNTDEFLDLLASSQSRRLDDQRASF
                     SNLPGLRLTQNSQSVLSHLMTNDNKEADEDFFDILVKCQGSRLDDQRCAPPPATTKGP
                     TVPDEDFFSLILRSQGKRMDEQRVLLQRDQNRDTDFGLKDFLQNNALLEFKNSGKKSA
                     DH"
     exon            549..770
                     /gene="GPSM2"
                     /gene_synonym="CMCS; DFNB82; LGN; PINS"
                     /inference="alignment:Splign:2.1.0"
     exon            771..906
                     /gene="GPSM2"
                     /gene_synonym="CMCS; DFNB82; LGN; PINS"
                     /inference="alignment:Splign:2.1.0"
     exon            907..1049
                     /gene="GPSM2"
                     /gene_synonym="CMCS; DFNB82; LGN; PINS"
                     /inference="alignment:Splign:2.1.0"
     exon            1050..1173
                     /gene="GPSM2"
                     /gene_synonym="CMCS; DFNB82; LGN; PINS"
                     /inference="alignment:Splign:2.1.0"
     exon            1174..1289
                     /gene="GPSM2"
                     /gene_synonym="CMCS; DFNB82; LGN; PINS"
                     /inference="alignment:Splign:2.1.0"
     exon            1290..1445
                     /gene="GPSM2"
                     /gene_synonym="CMCS; DFNB82; LGN; PINS"
                     /inference="alignment:Splign:2.1.0"
     exon            1446..1554
                     /gene="GPSM2"
                     /gene_synonym="CMCS; DFNB82; LGN; PINS"
                     /inference="alignment:Splign:2.1.0"
     exon            1555..1684
                     /gene="GPSM2"
                     /gene_synonym="CMCS; DFNB82; LGN; PINS"
                     /inference="alignment:Splign:2.1.0"
     exon            1685..1755
                     /gene="GPSM2"
                     /gene_synonym="CMCS; DFNB82; LGN; PINS"
                     /inference="alignment:Splign:2.1.0"
     exon            1756..1932
                     /gene="GPSM2"
                     /gene_synonym="CMCS; DFNB82; LGN; PINS"
                     /inference="alignment:Splign:2.1.0"
     exon            1933..2092
                     /gene="GPSM2"
                     /gene_synonym="CMCS; DFNB82; LGN; PINS"
                     /inference="alignment:Splign:2.1.0"
     exon            2093..2307
                     /gene="GPSM2"
                     /gene_synonym="CMCS; DFNB82; LGN; PINS"
                     /inference="alignment:Splign:2.1.0"
     exon            2308..7152
                     /gene="GPSM2"
                     /gene_synonym="CMCS; DFNB82; LGN; PINS"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2987..2992
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GPSM2"
                     /gene_synonym="CMCS; DFNB82; LGN; PINS"
                     /note="hexamer: TATAAA"
     polyA_site      3008
                     /gene="GPSM2"
                     /gene_synonym="CMCS; DFNB82; LGN; PINS"
                     /note="major polyA site"
     regulatory      6124..6129
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GPSM2"
                     /gene_synonym="CMCS; DFNB82; LGN; PINS"
                     /note="hexamer: AATAAA"
     polyA_site      6152
                     /gene="GPSM2"
                     /gene_synonym="CMCS; DFNB82; LGN; PINS"
     regulatory      6943..6948
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GPSM2"
                     /gene_synonym="CMCS; DFNB82; LGN; PINS"
                     /note="hexamer: AATAAA"
     polyA_site      6970
                     /gene="GPSM2"
                     /gene_synonym="CMCS; DFNB82; LGN; PINS"
     regulatory      7125..7130
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GPSM2"
                     /gene_synonym="CMCS; DFNB82; LGN; PINS"
                     /note="hexamer: AGTAAA"
     polyA_site      7152
                     /gene="GPSM2"
                     /gene_synonym="CMCS; DFNB82; LGN; PINS"
ORIGIN      
        1 gcagaggagg gcggtgttga gaccggcgga gcggcgggac ccctaggtgg cggagggacg
       61 ctccgggaaa gcgaggggcg ctacgagctc tggcccacgt gacctgccgg gggcgggagc
      121 agggggcgcg ccggcctcct gcggtgcccc tgccttgggg aggggccgtg accacccgtc
      181 tgtcgcccga ggcggccgcc gctgcacctt caccgcgtac ccgggacccg cccgcccgcg
      241 ggagaaatgt tgctgaagtg ctgctgaaag ggccagagat gcaaggattt gggatacatt
      301 ttgaaccttt aagctgtctg acattgacct cctttcatta ttaataaaga agaatcagga
      361 gcttaggatg tattaacacc aactcattaa tatactaacc ggacaatgtt ctacaaacaa
      421 ttctacattg taaaggactg gattggcaca aaataaaata attttatttt attcagctta
      481 taatatgact cgatggagga aaatttgata agcatgagag aagaccattc ttttcatgtt
      541 cgttacagaa tggaagcttc ttgcctagag ctggccttgg aaggggaacg tctatgtaaa
      601 tcaggagact gccgcgctgg cgtgtcattc tttgaagctg cagttcaagt tggaactgaa
      661 gacctaaaaa cacttagcgc tatttacagc cagttgggca atgcttattt ctatttgcat
      721 gattatgcca aagcattaga atatcaccat catgatttaa cccttgcaag gactattgga
      781 gaccagctgg gggaagcgaa agctagtggt aatctgggaa acaccttaaa agttcttggg
      841 aattttgacg aagccatagt ttgttgtcag cgacacctag atatttccag agagcttaat
      901 gacaaggtgg gagaagcaag agcactttac aatcttggga atgtgtatca tgccaaaggg
      961 aaaagttttg gttgccctgg tccccaggat gtaggagaat ttccagaaga agtgagagat
     1021 gctctgcagg cagccgtgga tttttatgag gaaaacctat cattagtgac tgctttgggt
     1081 gaccgagcgg cacaaggacg tgcctttgga aatcttggaa acacacatta cctccttggc
     1141 aacttcaggg atgcagttat agctcatgag cagcgtctcc ttattgcaaa agaatttgga
     1201 gataaagcag ctgaaagaag agcatatagc aaccttggaa atgcatatat atttcttggt
     1261 gaatttgaaa ctgcctcgga atactacaag aagacactac tgttggcccg acagcttaaa
     1321 gaccgagctg tagaagcaca gtcttgttac agtcttggaa atacatatac tttacttcaa
     1381 gactatgaaa aggccattga ttatcatctg aagcacttag caattgctca agagctgaat
     1441 gatagaattg gtgaaggaag agcatgttgg agcttaggaa atgcatacac agcactagga
     1501 aatcatgatc aagcaatgca ttttgctgaa aagcacttgg aaatttcaag agaggttggg
     1561 gataaaagtg gtgaactaac agcacgactt aatctctcag accttcaaat ggttcttggt
     1621 ctgagctaca gcacaaataa ctccataatg tctgaaaata ctgaaattga tagcagtttg
     1681 aatggtgtac gccccaagtt gggacgccgg catagtatgg aaaatatgga acttatgaag
     1741 ttaacaccag aaaaggtaca gaactggaac agtgaaattc ttgctaagca aaaacctctt
     1801 attgccaaac cttctgcaaa gctactcttt gtcaacagac tgaaggggaa aaaatacaaa
     1861 acgaattcct ccactaaagt tctccaagat gccagtaatt ctattgacca ccgaattcca
     1921 aattctcaga ggaaaatcag tgcagatact attggagatg aagggttctt tgacttatta
     1981 agccgatttc aaagcaatag gatggatgat cagagatgtt gcttacaaga aaagaactgc
     2041 catacagctt caacaacaac ttcttccact ccccctaaaa tgatgctaaa aacatcatct
     2101 gttcctgtgg tatcccccaa cacggatgag tttttagatc ttcttgccag ctcacagagt
     2161 cgccgtctgg atgaccagag ggctagtttc agtaatttgc cagggcttcg tctaacacaa
     2221 aacagccagt cggtacttag ccacctgatg actaatgaca acaaagaggc tgatgaagat
     2281 ttctttgaca tccttgtaaa atgtcaagga tccagattag atgatcaaag atgtgctcca
     2341 ccacctgcta ccacaaaggg tccgacagta ccagatgaag actttttcag ccttatttta
     2401 cggtcccagg gaaagagaat ggatgaacag agagttcttt tacaaagaga tcaaaacaga
     2461 gacactgact ttgggctaaa ggactttttg caaaataatg ctttgttgga gtttaaaaat
     2521 tcagggaaaa aatcggcaga ccattagtta ctatggattt attttttttc ctttcaaaca
     2581 cggtaaggaa acaatctatt acttttttcc ttaaaaggag aatttatagc actgtaatac
     2641 agcttaaaat atttttagaa tgatgtaaat agttaacctt cagtagtcta ttaaggcatt
     2701 aatacttctc tggacatgcg cgtttgaggg tggaggggtc ctgtaaggtg cttcatcgtc
     2761 tgtgattact gcttgggatg tgttctttgg cagcttgtga gattacttta cctagtgttt
     2821 ataaagtagg aagttaagtg aatcatagat tagaatttaa tactcttatg gaaataattt
     2881 tttaacatct taattgacaa tggcgttttt ttatacataa ccatggatgt agtgggaaac
     2941 aatgttgttt ggtaaaaata atgtacttga tcaatgtaaa aaagtatata aaatagtctt
     3001 actaaaaatc taggtttttt tttcctccat gaaaatctgt ttttttaggc caaagtcagt
     3061 ataaaagaaa ctctacttct tgggctgggc atggtggctc attcctgtaa tcccagcaca
     3121 ttgggaggct gaggcaggag gatcgcttga ggctaggagt tcaagaccaa cctgggcaac
     3181 atagaccccc gtgtctatga aaaataaatc agcagccagg cgcagtggct cacacctgta
     3241 atcccagcac tttgggaggc cgaagcgggc ggatcacttg aggtcgggag tttgagacca
     3301 gcctggccaa catagtgaaa ccctgtctct actaaaaata caaaaattag ctgggcatgg
     3361 tggcatgcgc ctgtagtttc agctacttgg gaggctgagg caggagaatc acttgaaccc
     3421 ctgggaggca gagattgcag tgagctgaga ccaagccact ggactccagc ctgagtgaca
     3481 gagcaagact ctgtctcaaa aaaaaaaaaa acagcaagca tgctggcaca cacctgtagt
     3541 cccaactgct taagaggctg aggcaggagg accacttgag cccaggaggt ggaggctgca
     3601 ctgagctatg atcgtgccac tgcactccag cctgggtgac agagcaagac cctgtctcta
     3661 gaaaacaagt atcttttgtg tgttttgggc tgtttaaaaa aattatttaa aatggtctct
     3721 tctgttccat aatactgctg taaaacaaac ttttctaagt tctaatataa aaacaaaaaa
     3781 caaaacccat gtggttaggt caagaaccta ggacacataa acaaacagaa aacagatgaa
     3841 aactcacaaa aattaaatat gaaagaaaga tgtcagctag aaccttagtt gtcattaagc
     3901 tttgtcttcc ttatcccaga tattgaaggc agtttacaag gggatgataa caaagtaact
     3961 aactaactgt agcaaaagac aagtatggga cagactggga cctggagtaa cactggatca
     4021 cagactgcaa aggcccacgc ttggaacaag ttgccaactt gtttcactct gcttgcttca
     4081 gactgtgcac agctgggatg ggatctgggg atgtggaccc ctattctttc aaaaagtcat
     4141 tcaggtgatt tggatgggca aatagaacta tttctctaat ggccaatgtt ttttaagagt
     4201 cataacctgg aattaattac attaagtgct cagctaaaaa aaaaaaaaaa gttctaaatt
     4261 acaacctgga ttacattatg tttcatgtat actataaggt atgatgtcct ggatagcatt
     4321 ttaaaaactc aggtaagttt catggggttc ttataagaaa attcagttaa gacaatatac
     4381 caaaccacaa cgtaaaaagt ttgtgtatac aaactttttg tattatttgt acacaaagta
     4441 caaatttgta tttgtacaca aagtacaaac tttgtgtatt attttgtgta tacaatagtt
     4501 cttacagcct gtaaacattc aacattattc catttaaaca ttgtttttaa cagctgtcta
     4561 ccctgttctg attttcctaa aagcttcaca ctatataggc tgggcacaat ggctcatgcc
     4621 tgtaatccca gcactttggg aggccgacaa gggcgggtca cttgaggtca ggagttcgag
     4681 accagcctga ccaacatggt gaaaccccat ctctactaaa aaatacaaaa attagccagg
     4741 catggtgatg cacatctgta atcctagcta ctcgggagac tgaggcagga gaatctcttg
     4801 aacctgggag gtggaggttg cagtgagccg agattgtacc acctcactcc agcctgggtg
     4861 acagagcaaa actctcaaaa aaaagtaaaa tttcaaaaaa gcttcacaat ataaacctta
     4921 attttaatga catattggct agtcaataaa caagtcttat ctcatctcat ctcttttctg
     4981 ataacaaaca cccgatgtgt tcaatttgct ttcatattta agtctttcct gaattgctgt
     5041 catcattcaa caacagttgc atgtcgcctt gctagctgtc aaagtagact tcatccccaa
     5101 atggatatct gtaatgaaag aatacaaagg tgaaatttta tttaaaaatt ttttaaaaga
     5161 atttggtttt ggattaaaag gcatgcaagc agcattaatt ccactcacag ttacagtcta
     5221 tcacctgggg cattcactac ttttcagagt cagatcacag ttcaaaagac agctctcacc
     5281 ttgggggcat attccccaag tcagtgtgaa catgtcttgt cccaagagga cactttatac
     5341 gcatggatgt tcacaaattt attctaaact gcaggtgtct gtcaggattc atttgttaaa
     5401 catgtcctct gtgttaaaaa tcctaaaatc cttttgcatt tcttagaaaa aatcacgttt
     5461 ctgatgaaat tctaaagatg tttgcaatat taaactagaa tatattaaca tgtataattt
     5521 tgagacaagg tcttgctctg tcacccaggc tggagtacag tggctcgatc ataactcact
     5581 gccacctcac gatcctcttg cctcagccct ccaagtagct gggactacag gcgtgtacca
     5641 ccatgcctgg ctagttttat ttttagtaca tagccttgaa caactgagct taagcgatca
     5701 tcctccctca accttccaaa gtgctaggac tgtaggcatg agccactgca cctggcctaa
     5761 aattacattg ttacaggcac gtaagtaaca cttcctggat ccaaaaatgt gtattctgca
     5821 tttcaacaac tgaacttaag cgatcatcct ccctcaacct tccaaagtgc taggactgta
     5881 ggcatgagcc actgcacctg gcctaaaatt acattgttac aggcacgtaa gtaacacttc
     5941 ctggatccaa aaatgtgtat tctgcatttc aacaactgaa cttaagcgat catcctccct
     6001 caaccttcca aagtgctagg actgtaggca tgagccactg cacctggcct aaaattacat
     6061 tgttacaggc acgtaagtaa cacttcctgg atccaaaaat gtgtattctg catttcatat
     6121 taaaataaaa aagcaagtgc ttgggataca aatatctgca tgaaaattta aagactttat
     6181 tcctgactgg tatcactttt agtaagcagt tgaacataac ttgtagtgtg aatatggtta
     6241 aaacaaagga cacctgatgt ctgtgaagtc tgctaaggac aggtacaaaa tttatgcatt
     6301 gctttttaaa aagtttaaaa tgaggaatgc ttttgataat cagaaagact aatgtaaagt
     6361 gctgactgat gtcctgtctg cagttaagga agacacccaa ctctcttctt cctcatcatg
     6421 gtattctcta tgtatagatc tctaaaaatg caaacttcct atggacaaga caatatgatt
     6481 tgctataata tgaattaaga tatggtaata tctaatagtc tccactgcta ggattctgag
     6541 taacacaaaa aataggtttt ataaaaagcc catgcacttc aattggtggg ggaaaagaat
     6601 aaagtcattt tcagtcgact gactctgtaa aacagattac caatataaca agctatgtta
     6661 tctaaattgc cctggcagtc ttcagttttc tctaagtaaa caaagtaaaa ggaacaataa
     6721 ggatgcaggc gtagtaatag ctgtggaaaa gatgaaacta cagccacagt gacctgtggg
     6781 cagcctgcgt agagataagc tgaggtgtca gtcagactga tcctggtaac gatactccat
     6841 ggctgcatga agatgctgga ggcatcaagt gggtggcctt aactctagtg gattcactag
     6901 gcaatgtaaa ttggttagtg ggttcccgta agtgcctgta acaataaaat ttctttttca
     6961 atgtccccaa ttcaaactgg cctccttaac tatccagaat cagtgatgcc tgtcatctgt
     7021 tcatcttaaa ataaacactt cttactttat gggatgtaaa tatcaaagag aagatgaaat
     7081 gaaaagcttt tacatatata acgtgtttgt taattctaat tgtcagtaaa aatgtgaatg
     7141 ttgaagttga aa
//
