LOCUS       NM_016235               5144 bp    mRNA    linear   PRI 13-APR-2024
DEFINITION  Homo sapiens G protein-coupled receptor class C group 5 member B
            (GPRC5B), transcript variant 1, mRNA.
ACCESSION   NM_016235
VERSION     NM_016235.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5144)
  AUTHORS   Passchier,E.M.J., Kerst,S., Brouwers,E., Hamilton,E.M.C.,
            Bisseling,Q., Bugiani,M., Waisfisz,Q., Kitchen,P., Unger,L.,
            Breur,M., Hoogterp,L., de Vries,S.I., Abbink,T.E.M., Kole,M.H.P.,
            Leurs,R., Vischer,H.F., Brignone,M.S., Ambrosini,E., Feillet,F.,
            Born,A.P., Epstein,L.G., Mansvelder,H.D., Min,R. and van der
            Knaap,M.S.
  TITLE     Aquaporin-4 and GPRC5B: old and new players in controlling brain
            oedema
  JOURNAL   Brain 146 (8), 3444-3454 (2023)
   PUBMED   37143309
REFERENCE   2  (bases 1 to 5144)
  AUTHORS   Kanamori,K., Ozawa,S., Iwabuchi,H., Ikoma,T., Suzuki,K., Tanaka,K.,
            Yoshimura Sawai,N., Abe,T., Kato,Y., Hata,R.I. and Kobayashi,M.
  TITLE     GPRC5B (G protein-coupled receptor class C group 5 member B)
            suppresses glucose starvation-induced apoptosis in head-and-neck
            squamous cell carcinoma
  JOURNAL   Biomed Res 44 (1), 1-7 (2023)
   PUBMED   36682796
  REMARK    GeneRIF: GPRC5B (G protein-coupled receptor class C group 5 member
            B) suppresses glucose starvation-induced apoptosis in head-and-neck
            squamous cell carcinoma.
REFERENCE   3  (bases 1 to 5144)
  AUTHORS   Huang,C., Zhang,N., Xiong,H., Wang,N., Chen,Z., Ni,Z., Liu,X.,
            Lin,B., Ge,B., Du,B. and Huang,Q.
  TITLE     Multi-Omics Analysis for Transcriptional Regulation of
            Immune-Related Targets Using Epigenetic Data: A New Research
            Direction
  JOURNAL   Front Immunol 12, 741634 (2022)
   PUBMED   35046932
  REMARK    GeneRIF: Multi-Omics Analysis for Transcriptional Regulation of
            Immune-Related Targets Using Epigenetic Data: A New Research
            Direction.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5144)
  AUTHORS   Alonso-Gardon,M., Elorza-Vidal,X., Castellanos,A., La Sala,G.,
            Armand-Ugon,M., Gilbert,A., Di Pietro,C., Pla-Casillanis,A.,
            Ciruela,F., Gasull,X., Nunes,V., Martinez,A., Schulte,U.,
            Cohen-Salmon,M., Marazziti,D. and Estevez,R.
  TITLE     Identification of the GlialCAM interactome: the G protein-coupled
            receptors GPRC5B and GPR37L1 modulate megalencephalic
            leukoencephalopathy proteins
  JOURNAL   Hum Mol Genet 30 (17), 1649-1665 (2021)
   PUBMED   34100078
  REMARK    GeneRIF: Identification of the GlialCAM interactome: the G
            protein-coupled receptors GPRC5B and GPR37L1 modulate
            megalencephalic leukoencephalopathy proteins.
REFERENCE   5  (bases 1 to 5144)
  AUTHORS   Soni,A., Amisten,S., Rorsman,P. and Salehi,A.
  TITLE     GPRC5B a putative glutamate-receptor candidate is negative
            modulator of insulin secretion
  JOURNAL   Biochem Biophys Res Commun 441 (3), 643-648 (2013)
   PUBMED   24404583
  REMARK    GeneRIF: this study proposes that increased expression of GPRC5B
            contributes to the reduced insulin secretion and beta-cell
            viability observed in type-2 diabetes.
REFERENCE   6  (bases 1 to 5144)
  AUTHORS   Palmer,D.J., Kelly,V.C., Smit,A.M., Kuy,S., Knight,C.G. and
            Cooper,G.J.
  TITLE     Human colostrum: identification of minor proteins in the aqueous
            phase by proteomics
  JOURNAL   Proteomics 6 (7), 2208-2216 (2006)
   PUBMED   16502470
REFERENCE   7  (bases 1 to 5144)
  AUTHORS   Robbins,M.J., Michalovich,D., Hill,J., Calver,A.R., Medhurst,A.D.,
            Gloger,I., Sims,M., Middlemiss,D.N. and Pangalos,M.N.
  TITLE     Molecular cloning and characterization of two novel retinoic
            acid-inducible orphan G-protein-coupled receptors (GPRC5B and
            GPRC5C)
  JOURNAL   Genomics 67 (1), 8-18 (2000)
   PUBMED   10945465
REFERENCE   8  (bases 1 to 5144)
  AUTHORS   Brauner-Osborne,H. and Krogsgaard-Larsen,P.
  TITLE     Sequence and expression pattern of a novel human orphan
            G-protein-coupled receptor, GPRC5B, a family C receptor with a
            short amino-terminal domain
  JOURNAL   Genomics 65 (2), 121-128 (2000)
   PUBMED   10783259
REFERENCE   9  (bases 1 to 5144)
  AUTHORS   Loftus,B.J., Kim,U.J., Sneddon,V.P., Kalush,F., Brandon,R.,
            Fuhrmann,J., Mason,T., Crosby,M.L., Barnstead,M., Cronin,L.,
            Deslattes Mays,A., Cao,Y., Xu,R.X., Kang,H.L., Mitchell,S.,
            Eichler,E.E., Harris,P.C., Venter,J.C. and Adams,M.D.
  TITLE     Genome duplications and other features in 12 Mb of DNA sequence
            from human chromosome 16p and 16q
  JOURNAL   Genomics 60 (3), 295-308 (1999)
   PUBMED   10493829
REFERENCE   10 (bases 1 to 5144)
  AUTHORS   Min,R., Abbink,T.E.M. and van der Knaap,M.S.
  TITLE     Megalencephalic Leukoencephalopathy with Subcortical Cysts
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE, Bean LJH,
            Gripp KW and Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301707
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA141861.1, BC034467.2 and
            AC134300.3.
            
            On Nov 23, 2018 this sequence version replaced NM_016235.2.
            
            Summary: This gene encodes a member of the type 3 G protein-coupled
            receptor family. Members of this superfamily are characterized by a
            signature 7-transmembrane domain motif. The encoded protein may
            modulate insulin secretion and increased protein expression is
            associated with type 2 diabetes. Alternative splicing results in
            multiple transcript variants. [provided by RefSeq, Feb 2015].
            
            Transcript Variant: This variant (1) represents the shorter
            transcript and encodes the shorter isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC034467.2, AF181862.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1968968,
                                           SAMEA1970526 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000300571.7/ ENSP00000300571.2
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-26                DA141861.1         51-76
            27-2864             BC034467.2         3-2840
            2865-5144           AC134300.3         84669-86948
FEATURES             Location/Qualifiers
     source          1..5144
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="16"
                     /map="16p12.3"
     gene            1..5144
                     /gene="GPRC5B"
                     /gene_synonym="MLC3; RAIG-2; RAIG2"
                     /note="G protein-coupled receptor class C group 5 member
                     B"
                     /db_xref="GeneID:51704"
                     /db_xref="HGNC:HGNC:13308"
                     /db_xref="MIM:605948"
     exon            1..122
                     /gene="GPRC5B"
                     /gene_synonym="MLC3; RAIG-2; RAIG2"
                     /inference="alignment:Splign:2.1.0"
     exon            123..1153
                     /gene="GPRC5B"
                     /gene_synonym="MLC3; RAIG-2; RAIG2"
                     /inference="alignment:Splign:2.1.0"
     CDS             124..1335
                     /gene="GPRC5B"
                     /gene_synonym="MLC3; RAIG-2; RAIG2"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; retinoic acid responsive gene protein; G
                     protein-coupled receptor, family C, group 1, member B;
                     G-protein coupled receptor family C group 5 member B;
                     retinoic acid-induced gene 2 protein"
                     /codon_start=1
                     /product="G-protein coupled receptor family C group 5
                     member B isoform 1 precursor"
                     /protein_id="NP_057319.1"
                     /db_xref="CCDS:CCDS10581.1"
                     /db_xref="GeneID:51704"
                     /db_xref="HGNC:HGNC:13308"
                     /db_xref="MIM:605948"
                     /translation="MFVASERKMRAHQVLTFLLLFVITSVASENASTSRGCGLDLLPQ
                     YVSLCDLDAIWGIVVEAVAGAGALITLLLMLILLVRLPFIKEKEKKSPVGLHFLFLLG
                     TLGLFGLTFAFIIQEDETICSVRRFLWGVLFALCFSCLLSQAWRVRRLVRHGTGPAGW
                     QLVGLALCLMLVQVIIAVEWLVLTVLRDTRPACAYEPMDFVMALIYDMVLLVVTLGLA
                     LFTLCGKFKRWKLNGAFLLITAFLSVLIWVAWMTMYLFGNVKLQQGDAWNDPTLAITL
                     AASGWVFVIFHAIPEIHCTLLPALQENTPNYFDTSQPRMRETAFEEDVQLPRAYMENK
                     AFSMDEHNAALRTAGFPNGSLGKRPSGSLGKRPSAPFRSNVYQPTEMAVVLNGGTIPT
                     APPSHTGRHLW"
     exon            1154..1290
                     /gene="GPRC5B"
                     /gene_synonym="MLC3; RAIG-2; RAIG2"
                     /inference="alignment:Splign:2.1.0"
     exon            1291..5144
                     /gene="GPRC5B"
                     /gene_synonym="MLC3; RAIG-2; RAIG2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2841..2846
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GPRC5B"
                     /gene_synonym="MLC3; RAIG-2; RAIG2"
                     /note="hexamer: AATAAA"
     polyA_site      2864
                     /gene="GPRC5B"
                     /gene_synonym="MLC3; RAIG-2; RAIG2"
                     /note="major polyA site"
     regulatory      4517..4522
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GPRC5B"
                     /gene_synonym="MLC3; RAIG-2; RAIG2"
                     /note="hexamer: AATAAA"
     polyA_site      4541
                     /gene="GPRC5B"
                     /gene_synonym="MLC3; RAIG-2; RAIG2"
     regulatory      5062..5067
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GPRC5B"
                     /gene_synonym="MLC3; RAIG-2; RAIG2"
                     /note="hexamer: AATAAA"
     polyA_site      5087
                     /gene="GPRC5B"
                     /gene_synonym="MLC3; RAIG-2; RAIG2"
     regulatory      5123..5128
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GPRC5B"
                     /gene_synonym="MLC3; RAIG-2; RAIG2"
                     /note="hexamer: ATTAAA"
     polyA_site      5144
                     /gene="GPRC5B"
                     /gene_synonym="MLC3; RAIG-2; RAIG2"
ORIGIN      
        1 gctgcagtga agctggaccc gcaggcgggc gtgcgtggag cgggggccgc ggccgcgccg
       61 cagagatgtg actcgggccg aaggccagct ggagcgtcgg cgctgcgggg ccgcgggggt
      121 cgaatgttcg tggcatcaga gagaaagatg agagctcacc aggtgctcac cttcctcctg
      181 ctcttcgtga tcacctcggt ggcctctgaa aacgccagca catcccgagg ctgtgggctg
      241 gacctcctcc ctcagtacgt gtccctgtgc gacctggacg ccatctgggg cattgtggtg
      301 gaggcggtgg ccggggcggg cgccctgatc acactgctcc tgatgctcat cctcctggtg
      361 cggctgccct tcatcaagga gaaggagaag aagagccctg tgggcctcca ctttctgttc
      421 ctcctgggga ccctgggcct ctttgggctg acgtttgcct tcatcatcca ggaggacgag
      481 accatctgct ctgtccgccg cttcctctgg ggcgtcctct ttgcgctctg cttctcctgc
      541 ctgctgagcc aggcatggcg cgtgcggagg ctggtgcggc atggcacggg ccccgcgggc
      601 tggcagctgg tgggcctggc gctgtgcctg atgctggtgc aagtcatcat cgctgtggag
      661 tggctggtgc tcaccgtgct gcgtgacaca aggccagcct gcgcctacga gcccatggac
      721 tttgtgatgg ccctcatcta cgacatggta ctgcttgtgg tcaccctggg gctggccctc
      781 ttcactctgt gcggcaagtt caagaggtgg aagctgaacg gggccttcct cctcatcaca
      841 gccttcctct ctgtgctcat ctgggtggcc tggatgacca tgtacctctt cggcaatgtc
      901 aagctgcagc agggggatgc ctggaacgac cccaccttgg ccatcacgct ggcggccagc
      961 ggctgggtct tcgtcatctt ccacgccatc cctgagatcc actgcaccct tctgccagcc
     1021 ctgcaggaga acacgcccaa ctacttcgac acgtcgcagc ccaggatgcg ggagacggcc
     1081 ttcgaggagg acgtgcagct gccgcgggcc tatatggaga acaaggcctt ctccatggat
     1141 gaacacaatg cagctctccg aacagcagga tttcccaacg gcagcttggg aaaaagaccc
     1201 agtggcagct tggggaaaag acccagcgct ccgtttagaa gcaacgtgta tcagccaact
     1261 gagatggccg tcgtgctcaa cggtgggacc atcccaactg ctccgccaag tcacacagga
     1321 agacaccttt ggtgaaagac tttaagttcc agagaatcag aatttctctt accgatttgc
     1381 ctccctggct gtgtctttct tgagggagaa atcggtaaca gttgccgaac caggccgcct
     1441 cacagccagg aaatttggaa atcctagcca aggggatttc gtgtaaatgt gaacactgac
     1501 gaactgaaaa gctaacaccg actgcccgcc cctcccctgc cacacacaca gacacgtaat
     1561 accagaccaa cctcaatccc cgcaaactaa agcaaagcta attgcaaata gtattaggct
     1621 cactggaaaa tgtggctggg aagactgttt catcctctgg gggtagaaca gaaccaaatt
     1681 cacagctggt gggccagact ggtgttggtt ggaggtgggg ggctcccact cttatcacct
     1741 ctccccagca agtgctggac cccaggtagc ctcttggaga tgaccgttgc gttgaggaca
     1801 aatggggact ttgccaccgg cttgcctggt ggtttgcaca tttcaggggg gtcaggagag
     1861 ttaaggaggt tgtgggtggg attccaaggt gaggcccaac tgaatcgtgg ggtgagcttt
     1921 atagccagta gaggtggagg gaccctggca tgtgccaaag aagaggccct ctgggtgatg
     1981 aagtgaccat cacatttgga aagtgatcaa ccactgttcc ttctatgggg ctcttgctct
     2041 agtgtctatg gtgagaacac aggccccgcc ccttcccttg tagagccata gaaatattct
     2101 ggcttggggc agcagtccct tcttcccttg atcatctcgc cctgttccta cacttacggg
     2161 tgtatctcca aatcctctcc caattttatt cccttattca tttcaagagc tccaatgggg
     2221 tctccagctg aaagcccctc cgggaggcag gttggaaggc aggcaccacg gcaggttttc
     2281 cgcgatgatg tcacctagca gggcttcagg ggttcccact aggatgcaga gatgacctct
     2341 cgctgcctca caagcagtga cacctcgggt cctttccgtt gctatggtga aaattcctgg
     2401 atggaatgga tcacatgagg gtttcttgtt gcttttggag ggtgtggggg atattttgtt
     2461 ttggtttttc tgcaggttcc atgaaaacag cccttttcca agcccattgt ttctgtcatg
     2521 gtttccatct gtcctgagca agtcattcct ttgttattta gcatttcgaa catctcggcc
     2581 attcaaagcc cccatgttct ctgcactgtt tggccagcat aacctctagc atcgattcaa
     2641 agcagagttt taacctgacg gcatggaatg tataaatgag ggtgggtcct tctgcagata
     2701 ctctaatcac tacattgctt tttctataaa actacccata agcctttaac ctttaaagaa
     2761 aaatgaaaaa ggttagtgtt tgggggccgg gggaggactg accgcttcat aagccagtac
     2821 gtctgagctg agtatgtttc aataaacctt ttgatatttc tcaaggccct agtctctgct
     2881 gtctcccctc cccaccccat ccttgcaaag cactggggaa agtaaggcca atctggccct
     2941 ccctgtgtga cccgccttcg agttttcctt aacagttagt acatttcctt gtgttaccac
     3001 gcatggggaa gaaaacgcat ggccccagaa tgccaccccc acctgacctc cccggaagca
     3061 ccccgcctct gcccagagca tgtgcttgct tctagagaat cccgttccag tcattgcgtg
     3121 gacagaaaac gtaagagtcc tggggagggg tgggagggaa tgaagctagg acctggggtg
     3181 ggggtggggg cggttgcatg cggacccggt attctgaaaa gaaagaaatc ttcaaacacc
     3241 tcggtcctgt ccggtgaaaa aacgacagca ttacacattc tcgtgccagg aaatggttac
     3301 tcacccccgc tctctggagt cctttggagc tttctgatac cattcacacg atggagcaat
     3361 tcacacgttc gttttctaag ttctttattg tccaagcata taaggtgagc acagcacaaa
     3421 tggctgccat ggaaagtcgt gcttccccag cataagaaca gagccatctt tgagccatgg
     3481 gaagcctcat ttaatgagac ctttttcccc ccttttaaat ctgatatgtt atcgcactta
     3541 gggttttgtt ttattttggc ttttaaggtt gagagtgggc tgaacgcctt cataatgggg
     3601 gcggaggcgg gagtgagagg agaagggaga gagctgttct ttttctaatg actacacctg
     3661 aaaagtatca agagacatct ttgccaagag actgtagcag ccagctgccc cccgtggagc
     3721 aaggtttaaa gacaaaatta aatggcacct ctgtttaaga tctgcgtgtg taaacaagac
     3781 tcgtttgggg aaaacgaccc gggaggagag catctgtgat caaaatctct cattgtaagc
     3841 acaaattgtt ccgtgtctgg ttattaaaat cgctttgggt ctataacagc cactcttgtc
     3901 ccccctttta atagaaaatt gtcattctag cctggatttc tccccactgg aggtggaggg
     3961 tgggaagaga agggagtcag ctctgacagc ttacaaactg ggaagttctg tgcatctcca
     4021 gggattccag agttgaagat ctggttgttg gaagctgggc gcccagtgct tttttttttt
     4081 ttttttttta gcccagatgc cgtcaggacc cactgaacaa gggtgaactc gggtggtggt
     4141 ctcggttggc caaggctgag ttgaacgcct caggccaaac cccaagttga aagggagcat
     4201 tgtggctttt ataatccaat gatgcaatga tgataccagc ttacaggagt tctcagcagg
     4261 tgctttttat taactgatga agagctaagg ctcacttttg atagataata tatatttatt
     4321 aaatgcatta atgtgtgttt tataatgtac ctttatcttc ccccggctga ctgttgcata
     4381 cttataaaaa agaagttaaa gaaatttcaa gacaatcaat atgtaccata aacgactata
     4441 taaatattac aaatttaaaa ggctgtaaac catgggcaga taggttttat tttcaaaatg
     4501 ctgttcttaa cacaaaaata aaggctttac tatttactta agacgtgtgg tagcttgtca
     4561 aaacaaccca gtgcaactaa ctactaggct ttcggatctg ccttgagggt cggtctgtgg
     4621 cattttaaat gggagaatct tggttaacag caaaccacag aacatattaa ggtaataagt
     4681 ttacccataa aagcaggaac tttctatata atactttggg acttagacaa caaaaatcct
     4741 ttgttacatc tgtctgggtt taagtgtctc atcttgttgg gtttgcagta aaagttaagg
     4801 agtggtctcc tttggaaatg atcattaagt ataaggtctt ttttataaag agagtagtca
     4861 gtcactaggt aaacttaaaa ccaaattcag aggttgggga cttaaattag aatatggatc
     4921 tttgcaaagg cggccacaag tgggaaaact ggtaataaga gcacttctgt attccttggg
     4981 actagagttc attgtacatt ttaacagcag ctggtgtaga agaatactcc tgaggcactg
     5041 aagaagtaat ctggatgaat gaataaaatg tggggataat gcagaaagtg aagactggtc
     5101 ctttcttttc taaactattt gcattaaagc ttgtttgcaa tgaa
//
