LOCUS       NM_001142524            5155 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens G protein-coupled receptor associated sorting protein
            family member 3 (GPRASP3), transcript variant 1, mRNA.
ACCESSION   NM_001142524 XM_005262204
VERSION     NM_001142524.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5155)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   2  (bases 1 to 5155)
  AUTHORS   Kutzner,A. and Heese,K.
  TITLE     Glutamate E15 and E171 are Hotspots in p60TRP-Related Cancer
  JOURNAL   Cancer Invest 34 (2), 64-69 (2016)
   PUBMED   26854063
  REMARK    GeneRIF: Report that some regions of p60TRP were more prone to
            specific mutations, with two hotspots for mutations at E15 and
            E171.
REFERENCE   3  (bases 1 to 5155)
  AUTHORS   Gonzales,P.A., Pisitkun,T., Hoffert,J.D., Tchapyjnikov,D.,
            Star,R.A., Kleta,R., Wang,N.S. and Knepper,M.A.
  TITLE     Large-scale proteomics and phosphoproteomics of urinary exosomes
  JOURNAL   J Am Soc Nephrol 20 (2), 363-379 (2009)
   PUBMED   19056867
REFERENCE   4  (bases 1 to 5155)
  AUTHORS   Winter,E.E. and Ponting,C.P.
  TITLE     Mammalian BEX, WEX and GASP genes: coding and non-coding chimaerism
            sustained by gene conversion events
  JOURNAL   BMC Evol Biol 5, 54 (2005)
   PUBMED   16221301
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 5155)
  AUTHORS   Heese,K., Yamada,T., Akatsu,H., Yamamoto,T., Kosaka,K., Nagai,Y.
            and Sawada,T.
  TITLE     Characterizing the new transcription regulator protein p60TRP
  JOURNAL   J Cell Biochem 91 (5), 1030-1042 (2004)
   PUBMED   15034937
  REMARK    GeneRIF: Down-regulation in the brain of Alzheimer's disease
            subjects point to a possible pivotal role of p60TRP in the control
            of cellular aging and survival.
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA104939.1, AB051488.1,
            BC041409.2 and AL035427.17.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_001142524.1.
            
            Summary: This gene is a member of a gene family which encodes
            proteins with a basic helix-loop-helix domain. Other members of
            this gene family encode proteins which function as transcription
            factors, either enhancing or inhibiting transcription depending on
            the activity of other DNA binding proteins. The coding region of
            this gene is located entirely within the terminal exon. The encoded
            protein may be involved in the survival of neurons (PMID:
            15034937). Multiple alternatively spliced variants, encoding the
            same protein, have been identified. [provided by RefSeq, Sep 2011].
            
            Transcript Variant: This variant (1) represents the longest
            transcript. Variants 1-8 encode the same protein.
            
            ##Evidence-Data-START##
            Transcript exon combination :: DA104939.1, SRR1803615.16260.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968540, SAMEA1970526
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000457056.6/ ENSP00000403226.1
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-587               DA104939.1         1-587
            588-3584            AB051488.1         361-3357
            3585-4056           BC041409.2         3502-3973
            4057-5155           AL035427.17        130660-131758
FEATURES             Location/Qualifiers
     source          1..5155
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="X"
                     /map="Xq22.1"
     gene            1..5155
                     /gene="GPRASP3"
                     /gene_synonym="BHLHB9; GASP3; p60TRP"
                     /note="G protein-coupled receptor associated sorting
                     protein family member 3"
                     /db_xref="GeneID:80823"
                     /db_xref="HGNC:HGNC:29353"
                     /db_xref="MIM:300921"
     exon            1..83
                     /gene="GPRASP3"
                     /gene_synonym="BHLHB9; GASP3; p60TRP"
                     /inference="alignment:Splign:2.1.0"
     exon            84..218
                     /gene="GPRASP3"
                     /gene_synonym="BHLHB9; GASP3; p60TRP"
                     /inference="alignment:Splign:2.1.0"
     exon            219..313
                     /gene="GPRASP3"
                     /gene_synonym="BHLHB9; GASP3; p60TRP"
                     /inference="alignment:Splign:2.1.0"
     exon            314..5155
                     /gene="GPRASP3"
                     /gene_synonym="BHLHB9; GASP3; p60TRP"
                     /inference="alignment:Splign:2.1.0"
     CDS             611..2254
                     /gene="GPRASP3"
                     /gene_synonym="BHLHB9; GASP3; p60TRP"
                     /note="protein BHLHB9; p60-like protein; transcription
                     regulator of 60 kDa; basic helix-loop-helix domain
                     containing, class B, 9;
                     p60-transcription-regulator-protein; basic
                     helix-loop-helix family member b9"
                     /codon_start=1
                     /product="G protein-coupled receptor associated sorting
                     protein 3"
                     /protein_id="NP_001135996.1"
                     /db_xref="GeneID:80823"
                     /db_xref="HGNC:HGNC:29353"
                     /db_xref="MIM:300921"
                     /translation="MAGTKNKTRAQAKTEKKAAIQAKAGAEREATGVVRPVAKTRAKA
                     KAKTGSKTDAVAEMKAVSKNKVVAETKEGALSEPKTLGKAMGDFTPKAGNESTSSTCK
                     NEAGTDAWFWAGEEATINSWFWNGEEAGNSFSTKNDKPEIGAQVCAEELEPAAGADCK
                     PRSGAEEEEEENVIGNWFWEGDDTSFDPNPKPVSRIVKPQPVYEINEKNRPKDWSEVT
                     IWPNAPAVTPAVLGFRSQAPSEASPPSYIVLASAEENACSLPVATACRPSRNTRSCSQ
                     PIPECRFDSDPCIQTIDEIRRQIRIREVNGIKPFACPCKMECYMDSEEFEKLVSLLKS
                     TTDPLIHKIARIAMGVHNVHPFAQEFINEVGVVTLIESLLSFPSPEMRKKTVITLNPP
                     SGDERQRKIELHVKHMCKETMSFPLNSPGQQSGLKILGQLTTDFVHHYIVANYFSELF
                     HLLSSGNCKTRNLVLKLLLNMSENPTAARDMINMKALAALKLIFNQKEAKANLVSGVA
                     IFINIKEHIRKGSIVVVDHLSYNTLMAIFREVKEIIETM"
     regulatory      4034..4039
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GPRASP3"
                     /gene_synonym="BHLHB9; GASP3; p60TRP"
                     /note="hexamer: AATAAA"
     polyA_site      4056
                     /gene="GPRASP3"
                     /gene_synonym="BHLHB9; GASP3; p60TRP"
                     /note="major polyA site"
     regulatory      5135..5140
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GPRASP3"
                     /gene_synonym="BHLHB9; GASP3; p60TRP"
                     /note="hexamer: AATAAA"
     polyA_site      5155
                     /gene="GPRASP3"
                     /gene_synonym="BHLHB9; GASP3; p60TRP"
                     /note="major polyA site"
ORIGIN      
        1 gcatccagaa aagacctgag aggcggacga cacctgtgct ccgcagagca gaacgtggga
       61 ggaacggcgg gatgttaagg taggagctca agaagcagag agtctacctg cccgacgcgt
      121 tcgtggagag gagcagcggt tgctgttgga ggaggtgggt gcaaggacgg cagatggcat
      181 ccctgagagg tggtgactga gactagatgc gggtgcaggt ctgtttcttt ctaggttcct
      241 gtagagatct gtatgtagct gttcacttgg aagttaatta agaaagaaca aaaactattc
      301 atttggattc aaggtccatc tccactggtg gctgtggtgg ctttggattg ttggaagacc
      361 atcaccttca gcaggtctgc acttgggaac aatcattctc ccccagcccg agcgtgcctc
      421 tgccctctgt atcattgaca gaggctgtat gtttggaaag agaactttgt gactgcagaa
      481 ctgacagttg actctaattc ttgctaccaa gtttttcccc gacccagtga aagagtgtta
      541 agacttcgac ctaggacaca ttgagaacca aggccaagac tggacagggc catataactg
      601 ggcttcaacc atggctggga ctaagaataa gacaagagcc caggccaaaa ctgaaaaaaa
      661 ggctgctata caagctaaag ctggagcaga gagggaggct actggtgttg ttaggcctgt
      721 agccaagacc agggccaaag caaaagccaa gacagggtct aagacagatg cagtagcaga
      781 gatgaaggca gtgtctaaga acaaggttgt tgctgagacg aaggaaggag ctctgtcaga
      841 gcctaagact ctgggcaaag ccatgggaga tttcactccc aaggctggga atgagtccac
      901 cagctccaca tgtaaaaatg aggctggtac tgatgcctgg ttctgggctg gggaagaggc
      961 cactatcaat tcctggttct ggaatggaga agaggctggt aatagtttca gcactaagaa
     1021 tgataaacct gaaattggtg cccaggtctg tgctgaggag ttggaacctg cggctggggc
     1081 cgattgcaaa cctaggtcag gggctgagga ggaggaggaa gagaatgtta ttgggaactg
     1141 gttttgggaa ggagatgata ctagttttga ccctaatcct aaacctgtga gcaggatagt
     1201 taagcctcag cctgtgtatg aaattaatga aaaaaatagg cccaaggact ggtctgaggt
     1261 aactatctgg cccaatgccc ctgctgtaac tccagctgtg ttaggattta gatcccaggc
     1321 accatctgag gcaagccctc cttcatatat tgttctggcc tccgctgaag aaaatgcctg
     1381 ttctttgcct gtggcaacag cttgccgccc ttctaggaac actcgctcat gctcacagcc
     1441 tatccctgag tgtcgttttg attctgaccc ctgcatccag accatagatg agattagacg
     1501 tcaaatcagg atcagggagg taaatgggat taagccattt gcttgtcctt gcaaaatgga
     1561 atgctatatg gattctgagg aatttgaaaa acttgttagc ttacttaagt caactactga
     1621 tcctcttatt cataaaatag cacggattgc aatgggtgtc cataatgttc acccatttgc
     1681 ccaagagttt attaacgaag taggtgtagt gacacttatt gaaagcttgc tcagttttcc
     1741 ttcccctgaa atgagaaaaa agactgtaat tactctgaat cctccttctg gggatgaaag
     1801 acaacgcaaa attgaattac atgttaagca tatgtgtaaa gaaaccatgt catttccttt
     1861 gaactcaccg ggacagcaat ctggattaaa gatactagga caactgacta ctgattttgt
     1921 ccatcactac attgttgcca attacttttc agagcttttc catttgctgt cctcaggaaa
     1981 ttgcaaaacc agaaatcttg ttttgaaact acttttaaat atgtctgaaa atccaactgc
     2041 agccagagac atgatcaata tgaaggcatt ggcagcatta aaactcatct ttaaccagaa
     2101 agaggcaaaa gccaatcttg ttagtggtgt ggccatattt attaacataa aggagcatat
     2161 cagaaaaggc tcaattgtag ttgttgatca cttgagttat aatacactca tggccatttt
     2221 cagggaagtt aaagagatta ttgaaacaat gtaaaatgag ccagagatag aacattttaa
     2281 gccatcttca aactctagca ggctgtacat tacagtgtac acattataca ctgcatcttt
     2341 aacacagagt cacctgtgac aggctctagg tttgagctag actattttgg gggtatcaaa
     2401 tgaatattat accttgggct gaaaatgttt gatttttatc ttgtctagat tggcatattt
     2461 ttaacatttt acttaagata gcaaaccagt tcgttttaag taagctaact tgttcattag
     2521 tatctgtggc ttaaaatggc aaaaaagaaa atatccttga gtttgtaatc tagttacaga
     2581 agtaaggcat acacacacac aaagataaca gtacctagag agagagtgtg tgtgagtgtg
     2641 cgtgtctctg tgtgtgcacg tgcacgctca tggccaaatg tgcgcactct acataaagga
     2701 ggcaggagtt cctataggct atttaatgta agagaaacta tttttctcct gttccagctg
     2761 tatcagatac tcgttccgca acacagaaat gactcagaat ctcagacaaa atgtattatt
     2821 tgttcaattt taattttgct actacattca taactcttaa attgttaggc tgtttcattt
     2881 acatcaaagt tatctcacaa aagagaaggc aggaaacgtt ttgtgagtgc ctattctatg
     2941 tcaaacactg tgttggcacc atattttaca agtttttttc ctcttctcac agtgatcttg
     3001 tgagttagtt acttatattt ttattagaac tcattattct gggtaccctc caatgagaat
     3061 tagagaggtt aaataccttt tcctagattc ccacagcagg aaggtggcat agctgttttg
     3121 tctgacacca gaacccatct caccacactg ctttacagtc ttcctgaagg acattttgag
     3181 gtgggggggc cttcaaagct cagagactgg tttgaatggt ttaattttgc aatggatcat
     3241 gtccatgcca ggtgttacaa ttcttaactt cctccaaatt cgtgtgtcca ttagacattt
     3301 ggctacatct ggctggaggt caggagaaaa ttctgaggta aatagatgga ttttatctgg
     3361 cagtgtcgaa aatagtagga gcctaaaact ttgttcaatg agtaagatgt agatttggag
     3421 tcatcagcag gggaaacttg ttagatgtcc tttgtatctt tttctatcca tttgtatttt
     3481 ttctttccca aagagctgaa atcattttat accctgaatt taaaaggtaa ttgctttttt
     3541 cctccttgcg aaatcatacc ttaatttttt tttttttttt ttttaccatt tatgatttat
     3601 tctcctatgt gtcatatgaa aggccaaggg gctttatctt ttactagtag gcaagcctgg
     3661 gcatttctgc atttgtgcca gctaacacta caaaatagac ttaattagag aaatttagac
     3721 ccagaattgc agcttcaaca acacaaaaca ggaaagctag aagaggttgc tgtgtcaacc
     3781 tacaagatag tgattcttgg aagttggtat ggaccagaaa gcattcccag gacatttagg
     3841 ctatggctga gttctctaag actcagggtg ggaattataa tccctggtct aagctgatat
     3901 gaaagtttct atgtgtgaaa atatggagtt atatgctttg agattttctg ccagttaaac
     3961 taaaacaaga tcatactgta aatgtttgta acttgcttcc ccccactgta tactgtaaac
     4021 atctttgcat gtcaataaat atgcctctac aacatatttt tgaatcactt aatatttttc
     4081 ccatgttaac agttggtata tagtgttgta aacctttatt gcatattctc ataacataca
     4141 atatacacat tcttttgcaa aaatgggaac acagtattga gacttgcctt ttcctcttaa
     4201 cacttatctt ttcaatttag tattatatag atctgtcaca tgctttttat ctaataactt
     4261 tatagaaata taattgccat acattaagct gcatgttgaa agtatacagt gtgatcagtt
     4321 ttggaatgta tatacacaca aaaccataac cacaatcaaa attgtaaata cattcatcac
     4381 ccaccaaagc tacttttttt ttgagacaga gtcttgctct gtcacccagg ctggagtaca
     4441 gtggcgcaat ctcggcccac tgcaacctct gcttcccagg ttcaagtgat tctcgtgcct
     4501 tagcctcctg agcagctggg actacaggtg catgccacca catccgacta attttttttt
     4561 ttattttttg tatttttagt agagacaggg tttcaccatg ttggccaggc tggtcttgaa
     4621 ctcctggtct caagtgatct acccatcttg gtctccctga gtgccaggat tacaggcgtg
     4681 agccaccatg cccagcctcc cccaaagctt cttaatgccc ttttgtaatc cctccctccc
     4741 ctctctccct gtgcccctaa cccaggcaac catgggacat tttctgacaa aatagaattg
     4801 ttttcatttc ctgtaattgt gtataaatgg actcatatag catgtactct ttggtcggga
     4861 ggggatctgg cttatttcac ttagtgtaat tatttgagat tcatccatat catgtgtctc
     4921 actatttcat tgctttttat tgccaagtag tattccacca tatggcttta ccacagttta
     4981 tttatccatt cacttattga tggacatttg ggctgttcag agtttggcca ttataagtaa
     5041 agcagctatg agcatttgtg tacaagtgtt tttatggaca taggctttca tatctttgga
     5101 atgacatcta acaatattga atcttcgacc catgaataaa atctctctcc actta
//
