LOCUS       NM_001184727            5901 bp    mRNA    linear   PRI 06-JUN-2024
DEFINITION  Homo sapiens G protein-coupled receptor associated sorting protein
            1 (GPRASP1), transcript variant 4, mRNA.
ACCESSION   NM_001184727
VERSION     NM_001184727.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5901)
  AUTHORS   Du,J., Chen,Y., Liu,G., Zeng,Q., Zhou,N. and Du,D.
  TITLE     Comprehensive pan-cancer analysis of role of GPRASP1, associated
            with clinical outcomes, immune microenvironment, and
            immunotherapeutic efficiency in pancreatic cancer
  JOURNAL   Pathol Res Pract 243, 154374 (2023)
   PUBMED   36801507
  REMARK    GeneRIF: Comprehensive pan-cancer analysis of role of GPRASP1,
            associated with clinical outcomes, immune microenvironment, and
            immunotherapeutic efficiency in pancreatic cancer.
REFERENCE   2  (bases 1 to 5901)
  AUTHORS   Zhang,T., Liu,G., Zhang,J., Chen,S., Deng,Z. and Xie,M.
  TITLE     GPRASP1 is a candidate anti-oncogene and correlates with immune
            microenvironment and immunotherapeutic efficiency in head and neck
            cancer
  JOURNAL   J Oral Pathol Med 52 (3), 232-244 (2023)
   PUBMED   36264603
  REMARK    GeneRIF: GPRASP1 is a candidate anti-oncogene and correlates with
            immune microenvironment and immunotherapeutic efficiency in head
            and neck cancer.
REFERENCE   3  (bases 1 to 5901)
  AUTHORS   Zheng,X., Chang,F., Rong,Y. and Tuszynski,G.P.
  TITLE     G-protein coupled receptor-associated sorting protein 1 (GASP-1), a
            ubiquitous tumor marker, promotes proliferation and invasion of
            triple negative breast cancer
  JOURNAL   Exp Mol Pathol 125, 104751 (2022)
   PUBMED   35122807
  REMARK    GeneRIF: G-protein coupled receptor-associated sorting protein 1
            (GASP-1), a ubiquitous tumor marker, promotes proliferation and
            invasion of triple negative breast cancer.
REFERENCE   4  (bases 1 to 5901)
  AUTHORS   Manfra,O., Van Craenenbroeck,K., Skieterska,K., Frimurer,T.,
            Schwartz,T.W., Levy,F.O. and Andressen,K.W.
  TITLE     Downregulation of 5-HT7 Serotonin Receptors by the Atypical
            Antipsychotics Clozapine and Olanzapine. Role of Motifs in the
            C-Terminal Domain and Interaction with GASP-1
  JOURNAL   ACS Chem Neurosci 6 (7), 1206-1218 (2015)
   PUBMED   25706089
  REMARK    GeneRIF: Study demonstrated that binding of clozapine or olanzapine
            to the 5-HT7 receptor leads to antagonist-mediated lysosomal
            degradation by exposing key residues in the C-terminal tail that
            interact with GASP-1
REFERENCE   5  (bases 1 to 5901)
  AUTHORS   He,C., Wei,Y., Sun,K., Li,B., Dong,X., Zou,Z., Liu,Y., Kinch,L.N.,
            Khan,S., Sinha,S., Xavier,R.J., Grishin,N.V., Xiao,G.,
            Eskelinen,E.L., Scherer,P.E., Whistler,J.L. and Levine,B.
  TITLE     Beclin 2 functions in autophagy, degradation of G protein-coupled
            receptors, and metabolism
  JOURNAL   Cell 154 (5), 1085-1099 (2013)
   PUBMED   23954414
REFERENCE   6  (bases 1 to 5901)
  AUTHORS   Bartlett,S.E., Enquist,J., Hopf,F.W., Lee,J.H., Gladher,F.,
            Kharazia,V., Waldhoer,M., Mailliard,W.S., Armstrong,R., Bonci,A.
            and Whistler,J.L.
  TITLE     Dopamine responsiveness is regulated by targeted sorting of D2
            receptors
  JOURNAL   Proc Natl Acad Sci U S A 102 (32), 11521-11526 (2005)
   PUBMED   16049099
  REMARK    GeneRIF: Modulation of the D2-GASP interaction is important for the
            functional down-regulation of D2 dopamine receptors.
REFERENCE   7  (bases 1 to 5901)
  AUTHORS   Ross,M.T., Grafham,D.V., Coffey,A.J., Scherer,S., McLay,K.,
            Muzny,D., Platzer,M., Howell,G.R., Burrows,C., Bird,C.P.,
            Frankish,A., Lovell,F.L., Howe,K.L., Ashurst,J.L., Fulton,R.S.,
            Sudbrak,R., Wen,G., Jones,M.C., Hurles,M.E., Andrews,T.D.,
            Scott,C.E., Searle,S., Ramser,J., Whittaker,A., Deadman,R.,
            Carter,N.P., Hunt,S.E., Chen,R., Cree,A., Gunaratne,P., Havlak,P.,
            Hodgson,A., Metzker,M.L., Richards,S., Scott,G., Steffen,D.,
            Sodergren,E., Wheeler,D.A., Worley,K.C., Ainscough,R.,
            Ambrose,K.D., Ansari-Lari,M.A., Aradhya,S., Ashwell,R.I.,
            Babbage,A.K., Bagguley,C.L., Ballabio,A., Banerjee,R., Barker,G.E.,
            Barlow,K.F., Barrett,I.P., Bates,K.N., Beare,D.M., Beasley,H.,
            Beasley,O., Beck,A., Bethel,G., Blechschmidt,K., Brady,N.,
            Bray-Allen,S., Bridgeman,A.M., Brown,A.J., Brown,M.J., Bonnin,D.,
            Bruford,E.A., Buhay,C., Burch,P., Burford,D., Burgess,J.,
            Burrill,W., Burton,J., Bye,J.M., Carder,C., Carrel,L., Chako,J.,
            Chapman,J.C., Chavez,D., Chen,E., Chen,G., Chen,Y., Chen,Z.,
            Chinault,C., Ciccodicola,A., Clark,S.Y., Clarke,G., Clee,C.M.,
            Clegg,S., Clerc-Blankenburg,K., Clifford,K., Cobley,V., Cole,C.G.,
            Conquer,J.S., Corby,N., Connor,R.E., David,R., Davies,J., Davis,C.,
            Davis,J., Delgado,O., Deshazo,D., Dhami,P., Ding,Y., Dinh,H.,
            Dodsworth,S., Draper,H., Dugan-Rocha,S., Dunham,A., Dunn,M.,
            Durbin,K.J., Dutta,I., Eades,T., Ellwood,M., Emery-Cohen,A.,
            Errington,H., Evans,K.L., Faulkner,L., Francis,F., Frankland,J.,
            Fraser,A.E., Galgoczy,P., Gilbert,J., Gill,R., Glockner,G.,
            Gregory,S.G., Gribble,S., Griffiths,C., Grocock,R., Gu,Y.,
            Gwilliam,R., Hamilton,C., Hart,E.A., Hawes,A., Heath,P.D.,
            Heitmann,K., Hennig,S., Hernandez,J., Hinzmann,B., Ho,S., Hoffs,M.,
            Howden,P.J., Huckle,E.J., Hume,J., Hunt,P.J., Hunt,A.R.,
            Isherwood,J., Jacob,L., Johnson,D., Jones,S., de Jong,P.J.,
            Joseph,S.S., Keenan,S., Kelly,S., Kershaw,J.K., Khan,Z.,
            Kioschis,P., Klages,S., Knights,A.J., Kosiura,A., Kovar-Smith,C.,
            Laird,G.K., Langford,C., Lawlor,S., Leversha,M., Lewis,L., Liu,W.,
            Lloyd,C., Lloyd,D.M., Loulseged,H., Loveland,J.E., Lovell,J.D.,
            Lozado,R., Lu,J., Lyne,R., Ma,J., Maheshwari,M., Matthews,L.H.,
            McDowall,J., McLaren,S., McMurray,A., Meidl,P., Meitinger,T.,
            Milne,S., Miner,G., Mistry,S.L., Morgan,M., Morris,S., Muller,I.,
            Mullikin,J.C., Nguyen,N., Nordsiek,G., Nyakatura,G., O'Dell,C.N.,
            Okwuonu,G., Palmer,S., Pandian,R., Parker,D., Parrish,J.,
            Pasternak,S., Patel,D., Pearce,A.V., Pearson,D.M., Pelan,S.E.,
            Perez,L., Porter,K.M., Ramsey,Y., Reichwald,K., Rhodes,S.,
            Ridler,K.A., Schlessinger,D., Schueler,M.G., Sehra,H.K.,
            Shaw-Smith,C., Shen,H., Sheridan,E.M., Shownkeen,R., Skuce,C.D.,
            Smith,M.L., Sotheran,E.C., Steingruber,H.E., Steward,C.A.,
            Storey,R., Swann,R.M., Swarbreck,D., Tabor,P.E., Taudien,S.,
            Taylor,T., Teague,B., Thomas,K., Thorpe,A., Timms,K., Tracey,A.,
            Trevanion,S., Tromans,A.C., d'Urso,M., Verduzco,D., Villasana,D.,
            Waldron,L., Wall,M., Wang,Q., Warren,J., Warry,G.L., Wei,X.,
            West,A., Whitehead,S.L., Whiteley,M.N., Wilkinson,J.E.,
            Willey,D.L., Williams,G., Williams,L., Williamson,A.,
            Williamson,H., Wilming,L., Woodmansey,R.L., Wray,P.W., Yen,J.,
            Zhang,J., Zhou,J., Zoghbi,H., Zorilla,S., Buck,D., Reinhardt,R.,
            Poustka,A., Rosenthal,A., Lehrach,H., Meindl,A., Minx,P.J.,
            Hillier,L.W., Willard,H.F., Wilson,R.K., Waterston,R.H., Rice,C.M.,
            Vaudin,M., Coulson,A., Nelson,D.L., Weinstock,G., Sulston,J.E.,
            Durbin,R., Hubbard,T., Gibbs,R.A., Beck,S., Rogers,J. and
            Bentley,D.R.
  TITLE     The DNA sequence of the human X chromosome
  JOURNAL   Nature 434 (7031), 325-337 (2005)
   PUBMED   15772651
REFERENCE   8  (bases 1 to 5901)
  AUTHORS   Heydorn,A., Sondergaard,B.P., Ersboll,B., Holst,B., Nielsen,F.C.,
            Haft,C.R., Whistler,J. and Schwartz,T.W.
  TITLE     A library of 7TM receptor C-terminal tails. Interactions with the
            proposed post-endocytic sorting proteins ERM-binding phosphoprotein
            50 (EBP50), N-ethylmaleimide-sensitive factor (NSF), sorting nexin
            1 (SNX1), and G protein-coupled receptor-associated sorting protein
            (GASP)
  JOURNAL   J Biol Chem 279 (52), 54291-54303 (2004)
   PUBMED   15452121
REFERENCE   9  (bases 1 to 5901)
  AUTHORS   Simonin,F., Karcher,P., Boeuf,J.J., Matifas,A. and Kieffer,B.L.
  TITLE     Identification of a novel family of G protein-coupled receptor
            associated sorting proteins
  JOURNAL   J Neurochem 89 (3), 766-775 (2004)
   PUBMED   15086532
REFERENCE   10 (bases 1 to 5901)
  AUTHORS   Whistler,J.L., Enquist,J., Marley,A., Fong,J., Gladher,F.,
            Tsuruda,P., Murray,S.R. and Von Zastrow,M.
  TITLE     Modulation of postendocytic sorting of G protein-coupled receptors
  JOURNAL   Science 297 (5581), 615-620 (2002)
   PUBMED   12142540
  REMARK    GeneRIF: identified a protein that binds preferentially to the
            cytoplasmic tail of the delta opioid receptor as a candidate
            heterotrimeric GTP-binding protein (G protein)-coupled
            receptor-associated sorting protein (GASP) [GASP]
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BX954816.1, BX489726.1,
            BC114552.1, AY044233.1 and AW293629.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_001184727.1.
            
            Summary: This gene encodes a member of the GPRASP (G
            protein-coupled receptor associated sorting protein) family. The
            protein may modulate lysosomal sorting and functional
            down-regulation of a variety of G-protein coupled receptors. It
            targets receptors for degradation in lysosomes. The receptors
            interacting with this sorting protein include D2 dopamine receptor
            (DRD2), delta opioid receptor (OPRD1), beta-2 adrenergic receptor
            (ADRB2), D4 dopamine receptor (DRD4) and cannabinoid 1 receptor
            (CB1R). Multiple alternatively spliced transcript variants encoding
            the same protein have been identified. [provided by RefSeq, May
            2010].
            
            Transcript Variant: This variant (4) has an additional exon in the
            5' UTR and encodes the same protein, as compared to variant 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803612.256013.1,
                                           SRR1803613.156277.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968189, SAMEA1968540
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000537097.2/ ENSP00000445683.1
            RefSeq Select criteria :: based on expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-60                BX954816.1         84-143
            61-587              BX489726.1         1-527
            588-5202            BC114552.1         303-4917
            5203-5421           AY044233.1         391-609
            5422-5901           AW293629.1         15-494              c
FEATURES             Location/Qualifiers
     source          1..5901
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="X"
                     /map="Xq22.1"
     gene            1..5901
                     /gene="GPRASP1"
                     /gene_synonym="GASP; GASP-1; GASP1"
                     /note="G protein-coupled receptor associated sorting
                     protein 1"
                     /db_xref="GeneID:9737"
                     /db_xref="HGNC:HGNC:24834"
                     /db_xref="MIM:300417"
     exon            1..113
                     /gene="GPRASP1"
                     /gene_synonym="GASP; GASP-1; GASP1"
                     /inference="alignment:Splign:2.1.0"
     exon            114..160
                     /gene="GPRASP1"
                     /gene_synonym="GASP; GASP-1; GASP1"
                     /inference="alignment:Splign:2.1.0"
     exon            161..251
                     /gene="GPRASP1"
                     /gene_synonym="GASP; GASP-1; GASP1"
                     /inference="alignment:Splign:2.1.0"
     exon            252..319
                     /gene="GPRASP1"
                     /gene_synonym="GASP; GASP-1; GASP1"
                     /inference="alignment:Splign:2.1.0"
     exon            320..397
                     /gene="GPRASP1"
                     /gene_synonym="GASP; GASP-1; GASP1"
                     /inference="alignment:Splign:2.1.0"
     exon            398..5901
                     /gene="GPRASP1"
                     /gene_synonym="GASP; GASP-1; GASP1"
                     /inference="alignment:Splign:2.1.0"
     CDS             732..4919
                     /gene="GPRASP1"
                     /gene_synonym="GASP; GASP-1; GASP1"
                     /codon_start=1
                     /product="G-protein coupled receptor-associated sorting
                     protein 1"
                     /protein_id="NP_001171656.1"
                     /db_xref="CCDS:CCDS35352.1"
                     /db_xref="GeneID:9737"
                     /db_xref="HGNC:HGNC:24834"
                     /db_xref="MIM:300417"
                     /translation="MTGAEIESGAQVKPEKKPGEEVVGGAEIENDVPLVVRPKVRTQA
                     QIMPGARPKNKSKVMPGASTKVETSAVGGARPKSKAKAIPVSRFKEEAQMWAQPRFGA
                     ERLSKTERNSQTNIIASPLVSTDSVLVAKTKYLSEDRELVNTDTESFPRRKAHYQAGF
                     QPSFRSKEETNMGSWCCPRPTSKQEASPNSDFKWVDKSVSSLFWSGDEVTAKFHPGNR
                     VKDSNRSMHMANQEANTMSRSQTNQELYIASSSGSEDESVKTPWFWARDKTNTWSGPR
                     EDPNSRSRFRSKKEVYVESSSGSEHEDHLESWFGAGKEAKFRSKMRAGKEANNRARHR
                     AKREACIDFMPGSIDVIKKESCFWPEENANTFSRPMIKKEARARAMTKEEAKTKARAR
                     AKQEARSEEEALIGTWFWATDESSMADEASIESSLQVEDESIIGSWFWTEEEASMGTG
                     ASSKSRPRTDGERIGDSLFGAREKTSMKTGAEATSESILAADDEQVIIGSWFWAGEEV
                     NQEAEEETIFGSWFWVIDAASVESGVGVSCESRTRSEEEEVIGPWFWSGEQVDIEAGI
                     GEEARPGAEEETIFGSWFWAENQTYMDCRAETSCDTMQGAEEEEPIIGSWFWTRVEAC
                     VEGDVNSKSSLEDKEEAMIPCFGAKEEVSMKHGTGVRCRFMAGAEETNNKSCFWAEKE
                     PCMYPAGGGSWKSRPEEEEDIVNSWFWSRKYTKPEAIIGSWLWATEESNIDGTGEKAK
                     LLTEEETIINSWFWKEDEAISEATDREESRPEAEEGDIIGSWFWAGEEDRLEPAAETR
                     EEDRLAAEKEGIVGSWFGAREETIRREAGSCSKSSPKAEEEEVIIGSWFWEEEASPEA
                     VAGVGFESKPGTEEEEITVGSWFWPEEEASIQAGSQAVEEMESETEEETIFGSWFWDG
                     KEVSEEAGPCCVSKPEDDEEMIVESWFWSRDKAIKETGTVATCESKPENEEGAIVGSW
                     FEAEDEVDNRTDNGSNCGSRTLADEDEAIVGSWFWAGDEAHFESNPSPVFRAICRSTC
                     SVEQEPDPSRRPQSWEEVTVQFKPGPWGRVGFPSISPFRFPKEAASLFCEMFGGKPRN
                     MVLSPEGEDQESLLQPDQPSPEFPFQYDPSYRSVQEIREHLRAKESTEPESSSCNCIQ
                     CELKIGSEEFEELLLLMEKIRDPFIHEISKIAMGMRSASQFTRDFIRDSGVVSLIETL
                     LNYPSSRVRTSFLENMIRMAPPYPNLNIIQTYICKVCEETLAYSVDSPEQLSGIRMIR
                     HLTTTTDYHTLVANYMSGFLSLLATGNAKTRFHVLKMLLNLSENLFMTKELLSAEAVS
                     EFIGLFNREETNDNIQIVLAIFENIGNNIKKETVFSDDDFNIEPLISAFHKVEKFAKE
                     LQGKTDNQNDPEGDQEN"
     regulatory      5246..5251
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GPRASP1"
                     /gene_synonym="GASP; GASP-1; GASP1"
                     /note="hexamer: AATAAA"
     polyA_site      5275
                     /gene="GPRASP1"
                     /gene_synonym="GASP; GASP-1; GASP1"
                     /note="major polyA site"
     regulatory      5877..5882
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GPRASP1"
                     /gene_synonym="GASP; GASP-1; GASP1"
                     /note="hexamer: AATAAA"
     polyA_site      5901
                     /gene="GPRASP1"
                     /gene_synonym="GASP; GASP-1; GASP1"
ORIGIN      
        1 gtctttgcca ttcggatcgc tgggaaagcg gtgggaatcc aactgaagag cagccagagg
       61 agagctgaag agaggagggg gaggccgatg acctgggctc tgggcctctg aagcctggca
      121 acgaggagac gatccgttcg gagccgggcg ctagagagag gtctggcgta ttctgacagg
      181 acacagtgag catctgtaga ggagaggctt gaaataaagg aggagcacga atattccctg
      241 gatttctgga ggcctgcttt aaggctggcc agttctgcaa gaaaggcaag gaggaggaga
      301 ctggctcaca cctctggagg acccccttct gtcagctgtg gggcttgaca ctacttgaac
      361 aagaaaagga gggggaaact gcaccacata agtgaagatc cacctccagt ggctgctctg
      421 ctggtggtgg ggttgctgct gacaaccacc ctcaacgggt ctgcacccat ccaggaaatc
      481 tctgtcttcc tcaagcttgg ttgtgcctgt tctacactct atctgtatta ttgaattact
      541 gactgagact gtgtttggga aggaggctga gtgactactg gactggatat tgactctaac
      601 tcttgtttcc aagcttatat cctcaatcac ctaaagatca gagtgtgaag aaacaaacct
      661 gtgacagatc tgtggttgag gtttagactg ggggaggagt atagtactgg actttctttg
      721 taacttgtac catgactggg gcagagattg agtctggtgc ccaggtcaag cctgaaaaga
      781 agcctgggga agaggttgta ggtggggctg agatagagaa tgatgtccct ctggtggtca
      841 gacccaaggt taggacccag gcccagataa tgcctggggc aaggcccaag aataagtcca
      901 aggttatgcc tggagcaagc accaaagttg agacaagtgc agtgggtggg gcacgcccta
      961 agagtaaggc caaggcaata cctgtttcac gatttaagga agaagcccag atgtgggctc
     1021 agcccaggtt tggtgctgaa agattgtcta agacagagag aaactcccag accaatatca
     1081 tagcctctcc acttgtcagt actgattctg tcttggttgc taaaacaaag tacctgtctg
     1141 aggatagaga actggttaat acagacactg agagctttcc tagaaggaag gcccattacc
     1201 aagcaggatt ccagccttct tttaggtcaa aggaggagac caatatgggg tcctggtgct
     1261 gtcctaggcc tacatccaaa caagaagcct ctcctaattc tgatttcaaa tgggtagaca
     1321 aatctgtgag ttccttgttc tggagtggag atgaggtcac tgcaaaattt catcctggga
     1381 atagggtaaa agacagtaac agatccatgc acatggccaa tcaagaggct aataccatgt
     1441 ctaggtccca aactaaccag gagctctata ttgcatctag ttctggttct gaggatgagt
     1501 ctgttaagac accctggttc tgggccagag ataaaaccaa tacctggtct gggcccaggg
     1561 aagatcccaa tagcaggtcc aggtttaggt ctaagaaaga agtctatgtt gaatcaagtt
     1621 ctggatctga gcatgaagac catttggagt cctggtttgg ggctggaaag gaggccaaat
     1681 tcaggtccaa aatgagagct gggaaggagg ccaataacag ggccaggcac agggccaagc
     1741 gagaagcttg cattgatttc atgcctgggt ctatagatgt aattaaaaaa gagtcctgtt
     1801 tctggcctga agaaaatgct aatacctttt caaggcccat gatcaagaaa gaggccaggg
     1861 ccagagcaat gacaaaggaa gaggccaaaa ccaaggcccg agccagggcc aagcaagaag
     1921 ccaggtcaga ggaggaagcc ctcattggga cctggttctg ggctacagac gagtccagca
     1981 tggcagatga agccagcata gagtccagtc tacaagtgga ggatgagtcc ataattggga
     2041 gttggttctg gactgaagaa gaggccagta tggggactgg ggctagcagt aaatccagac
     2101 caaggactga tggggagcgt attggtgatt ccttatttgg ggctagggaa aagaccagta
     2161 tgaaaactgg ggctgaggcc acctctgaat ctatactagc agctgatgat gaacaggtca
     2221 ttattggttc ctggttctgg gctggtgaag aggtcaacca agaggctgag gaagagacca
     2281 tttttgggtc gtggttctgg gtcattgatg cggccagtgt ggaatctggt gttggggtca
     2341 gctgtgagtc caggacaagg tctgaggaag aagaggtcat tggtccctgg ttttggtctg
     2401 gagaacaagt tgatatagag gctggaatcg gagaagaggc caggccagga gctgaagaag
     2461 agacaatatt cgggtcctgg ttttgggctg aaaaccagac ctatatggat tgtagggctg
     2521 aaactagctg tgacaccatg caaggggctg aggaggagga gcccattatt gggtcctggt
     2581 tttggaccag agtagaagct tgtgtggagg gtgatgtcaa cagcaagtct agcctggagg
     2641 acaaggaaga ggccatgata ccatgttttg gagccaaaga agaggtcagt atgaagcatg
     2701 ggactggtgt cagatgcaga tttatggcag gggctgagga gaccaataat aagtcttgct
     2761 tctgggcaga aaaagaaccc tgtatgtatc ctgccggtgg aggaagttgg aagtctaggc
     2821 cagaggagga agaggacatt gtcaattcgt ggttctggtc cagaaaatac acaaagccag
     2881 aggccattat agggtcctgg ttatgggcta cagaagagag taatatagat gggactggag
     2941 aaaaggccaa gttactgact gaagaggaga ccataatcaa ttcctggttc tggaaagaag
     3001 atgaagccat ttcagaggct actgacagag aagagtccag gccagaagct gaggaggggg
     3061 acattattgg ttcttggttc tgggctggag aagaggacag actagagcca gctgctgaga
     3121 ctagagaaga agacaggcta gcagctgaga aagaaggtat tgttgggtcc tggtttgggg
     3181 ccagagaaga gaccattaga agagaggctg ggtcttgcag caaatccagt cctaaagctg
     3241 aagaggaaga agtcattatt gggtcctggt tctgggaaga agaggccagt ccggaggcag
     3301 tggcaggagt cggctttgag tcaaagcctg ggactgagga ggaagaaatc actgttgggt
     3361 cctggttctg gcctgaagaa gaagccagta tacaggctgg atctcaggca gtagaggaaa
     3421 tggagtcaga gactgaagag gaaaccattt ttgggtcctg gttctgggat ggaaaagaag
     3481 tcagtgaaga agcaggacca tgctgtgtat ccaagccaga ggatgatgaa gagatgattg
     3541 ttgagtcctg gttctggtct agagacaaag ccattaagga aactggaact gtggccacct
     3601 gtgagtccaa gccagaaaat gaggaagggg ccattgttgg gtcttggttt gaggctgaag
     3661 atgaggtaga taacaggact gacaatggaa gcaactgtgg gtccaggaca ttagctgatg
     3721 aagatgaggc catagtgggg tcctggttct gggcaggaga tgaggcccat tttgaatcaa
     3781 atcctagccc cgtgttcagg gccatttgca ggtccacgtg ttcagttgaa caggagcctg
     3841 atccttcacg caggcctcag agttgggagg aggtcactgt tcagttcaag cctggtccat
     3901 ggggtagggt cggcttccca tctataagcc cctttagatt tccgaaagag gcagcatctt
     3961 tattctgtga aatgtttggg ggcaaaccca ggaacatggt acttagccca gaaggggaag
     4021 atcaggaatc tttgcttcag cctgatcagc ctagtcctga gttcccattt cagtatgatc
     4081 cttcctacag gtcagtccag gaaattcgag agcatcttag ggccaaggag agtacagagc
     4141 ctgagagttc atcctgtaac tgcatacaat gtgagctgaa aattggttct gaagagtttg
     4201 aagaactcct tttattaatg gaaaaaattc gggatccttt tattcatgaa atatctaaaa
     4261 tcgcaatggg tatgagaagt gcttctcaat ttacccgaga tttcattcga gattcaggtg
     4321 ttgtctcact tattgaaacc ttgcttaatt atccgtcctc ccgagttaga acaagttttt
     4381 tggaaaatat gattcgcatg gccccacctt atccgaatct aaacataatt cagacataca
     4441 tatgtaaagt gtgtgaggaa acccttgctt atagcgtgga ttccccggaa cagctgtctg
     4501 gaataaggat gattagacat ctcactacta ctactgacta tcacacactg gttgccaatt
     4561 atatgtctgg gtttctctcc ttattagcta caggcaatgc caaaacaagg tttcatgttt
     4621 tgaaaatgct actgaatttg tctgaaaatc ttttcatgac aaaagaacta ctcagtgctg
     4681 aagcagtgtc agaatttata ggcctcttta acagggaaga gacaaatgac aatattcaaa
     4741 ttgttcttgc aatatttgag aatattggca acaatatcaa aaaagaaaca gtgttctctg
     4801 atgatgattt caatattgag ccgcttattt ctgcattcca caaagttgag aaatttgcta
     4861 aggaactgca aggcaaaaca gacaatcaaa atgaccctga aggggaccaa gaaaattagt
     4921 aatggttaat tgctggcctc agattgtcct tatgttcctg agttatgatc cttgagtaat
     4981 gctttgattt taatagttgg ttctgtgttg caacatatat ctttagtgct gacactaact
     5041 ttgtccaact ctgtctgtaa gctggagcat ttttctgatg ccagctgaat attagagctg
     5101 aaaacacatt tgttgatatt tgtcttgtcc acattgtgat gttcagtatt tgagcttata
     5161 gtgaactgag caatcataaa taagccaccc ttctgattgt cgttctactg tatatatata
     5221 tatatttgag tgttgtttgt gtttcaataa agtcctatgt taaagttggc agaaatcacc
     5281 cttcttcttg aaattaaaat acagacccaa tgataacaca tgaacacaca tagagataat
     5341 taatatatga ataatatgtt cctttcaaaa gtttttttct cagctttaaa cgcaaaaatt
     5401 accaggggct gctatgtagg gcatacttca gagaaaaagt agctacttcg gttggactct
     5461 gaagtttggg aaagatattg gtggtgtaaa aaaaaaaaaa agcatggagt taggaaaaat
     5521 tttcctatgg gagtgaggta tcctgtttgc ctagagtgag tgagaggaat atattgagca
     5581 acatagagga tgacataagt tttaaagagc tgaggatttg cattttacag ctcaaagtac
     5641 aactgctgct tccatactac ctaaagggca aaagaataag acaggaaagc atagggattt
     5701 gattgagtca ggaagacaat ttaatcatga tggtaccatg gtaagtgtta aggcaacatc
     5761 atacagccat taaacatgat gatgtatagg gttttaggaa aagccctgac atttttgata
     5821 tttcatataa tagagaacac tggtgtcata catgggaagt atgatcccat ttgaaaaata
     5881 aatgtgaata aattgaccaa a
//
