LOCUS       NP_001171656            1395 aa            linear   PRI 27-APR-2025
DEFINITION  G-protein coupled receptor-associated sorting protein 1 [Homo
            sapiens].
ACCESSION   NP_001171656
VERSION     NP_001171656.1
DBSOURCE    REFSEQ: accession NM_001184727.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (residues 1 to 1395)
  AUTHORS   Du,J., Chen,Y., Liu,G., Zeng,Q., Zhou,N. and Du,D.
  TITLE     Comprehensive pan-cancer analysis of role of GPRASP1, associated
            with clinical outcomes, immune microenvironment, and
            immunotherapeutic efficiency in pancreatic cancer
  JOURNAL   Pathol Res Pract 243, 154374 (2023)
   PUBMED   36801507
  REMARK    GeneRIF: Comprehensive pan-cancer analysis of role of GPRASP1,
            associated with clinical outcomes, immune microenvironment, and
            immunotherapeutic efficiency in pancreatic cancer.
REFERENCE   2  (residues 1 to 1395)
  AUTHORS   Zhang,T., Liu,G., Zhang,J., Chen,S., Deng,Z. and Xie,M.
  TITLE     GPRASP1 is a candidate anti-oncogene and correlates with immune
            microenvironment and immunotherapeutic efficiency in head and neck
            cancer
  JOURNAL   J Oral Pathol Med 52 (3), 232-244 (2023)
   PUBMED   36264603
  REMARK    GeneRIF: GPRASP1 is a candidate anti-oncogene and correlates with
            immune microenvironment and immunotherapeutic efficiency in head
            and neck cancer.
REFERENCE   3  (residues 1 to 1395)
  AUTHORS   Zheng,X., Chang,F., Rong,Y. and Tuszynski,G.P.
  TITLE     G-protein coupled receptor-associated sorting protein 1 (GASP-1), a
            ubiquitous tumor marker, promotes proliferation and invasion of
            triple negative breast cancer
  JOURNAL   Exp Mol Pathol 125, 104751 (2022)
   PUBMED   35122807
  REMARK    GeneRIF: G-protein coupled receptor-associated sorting protein 1
            (GASP-1), a ubiquitous tumor marker, promotes proliferation and
            invasion of triple negative breast cancer.
REFERENCE   4  (residues 1 to 1395)
  AUTHORS   Manfra,O., Van Craenenbroeck,K., Skieterska,K., Frimurer,T.,
            Schwartz,T.W., Levy,F.O. and Andressen,K.W.
  TITLE     Downregulation of 5-HT7 Serotonin Receptors by the Atypical
            Antipsychotics Clozapine and Olanzapine. Role of Motifs in the
            C-Terminal Domain and Interaction with GASP-1
  JOURNAL   ACS Chem Neurosci 6 (7), 1206-1218 (2015)
   PUBMED   25706089
  REMARK    GeneRIF: Study demonstrated that binding of clozapine or olanzapine
            to the 5-HT7 receptor leads to antagonist-mediated lysosomal
            degradation by exposing key residues in the C-terminal tail that
            interact with GASP-1
REFERENCE   5  (residues 1 to 1395)
  AUTHORS   He,C., Wei,Y., Sun,K., Li,B., Dong,X., Zou,Z., Liu,Y., Kinch,L.N.,
            Khan,S., Sinha,S., Xavier,R.J., Grishin,N.V., Xiao,G.,
            Eskelinen,E.L., Scherer,P.E., Whistler,J.L. and Levine,B.
  TITLE     Beclin 2 functions in autophagy, degradation of G protein-coupled
            receptors, and metabolism
  JOURNAL   Cell 154 (5), 1085-1099 (2013)
   PUBMED   23954414
REFERENCE   6  (residues 1 to 1395)
  AUTHORS   Bartlett,S.E., Enquist,J., Hopf,F.W., Lee,J.H., Gladher,F.,
            Kharazia,V., Waldhoer,M., Mailliard,W.S., Armstrong,R., Bonci,A.
            and Whistler,J.L.
  TITLE     Dopamine responsiveness is regulated by targeted sorting of D2
            receptors
  JOURNAL   Proc Natl Acad Sci U S A 102 (32), 11521-11526 (2005)
   PUBMED   16049099
  REMARK    GeneRIF: Modulation of the D2-GASP interaction is important for the
            functional down-regulation of D2 dopamine receptors.
REFERENCE   7  (residues 1 to 1395)
  AUTHORS   Ross,M.T., Grafham,D.V., Coffey,A.J., Scherer,S., McLay,K.,
            Muzny,D., Platzer,M., Howell,G.R., Burrows,C., Bird,C.P.,
            Frankish,A., Lovell,F.L., Howe,K.L., Ashurst,J.L., Fulton,R.S.,
            Sudbrak,R., Wen,G., Jones,M.C., Hurles,M.E., Andrews,T.D.,
            Scott,C.E., Searle,S., Ramser,J., Whittaker,A., Deadman,R.,
            Carter,N.P., Hunt,S.E., Chen,R., Cree,A., Gunaratne,P., Havlak,P.,
            Hodgson,A., Metzker,M.L., Richards,S., Scott,G., Steffen,D.,
            Sodergren,E., Wheeler,D.A., Worley,K.C., Ainscough,R.,
            Ambrose,K.D., Ansari-Lari,M.A., Aradhya,S., Ashwell,R.I.,
            Babbage,A.K., Bagguley,C.L., Ballabio,A., Banerjee,R., Barker,G.E.,
            Barlow,K.F., Barrett,I.P., Bates,K.N., Beare,D.M., Beasley,H.,
            Beasley,O., Beck,A., Bethel,G., Blechschmidt,K., Brady,N.,
            Bray-Allen,S., Bridgeman,A.M., Brown,A.J., Brown,M.J., Bonnin,D.,
            Bruford,E.A., Buhay,C., Burch,P., Burford,D., Burgess,J.,
            Burrill,W., Burton,J., Bye,J.M., Carder,C., Carrel,L., Chako,J.,
            Chapman,J.C., Chavez,D., Chen,E., Chen,G., Chen,Y., Chen,Z.,
            Chinault,C., Ciccodicola,A., Clark,S.Y., Clarke,G., Clee,C.M.,
            Clegg,S., Clerc-Blankenburg,K., Clifford,K., Cobley,V., Cole,C.G.,
            Conquer,J.S., Corby,N., Connor,R.E., David,R., Davies,J., Davis,C.,
            Davis,J., Delgado,O., Deshazo,D., Dhami,P., Ding,Y., Dinh,H.,
            Dodsworth,S., Draper,H., Dugan-Rocha,S., Dunham,A., Dunn,M.,
            Durbin,K.J., Dutta,I., Eades,T., Ellwood,M., Emery-Cohen,A.,
            Errington,H., Evans,K.L., Faulkner,L., Francis,F., Frankland,J.,
            Fraser,A.E., Galgoczy,P., Gilbert,J., Gill,R., Glockner,G.,
            Gregory,S.G., Gribble,S., Griffiths,C., Grocock,R., Gu,Y.,
            Gwilliam,R., Hamilton,C., Hart,E.A., Hawes,A., Heath,P.D.,
            Heitmann,K., Hennig,S., Hernandez,J., Hinzmann,B., Ho,S., Hoffs,M.,
            Howden,P.J., Huckle,E.J., Hume,J., Hunt,P.J., Hunt,A.R.,
            Isherwood,J., Jacob,L., Johnson,D., Jones,S., de Jong,P.J.,
            Joseph,S.S., Keenan,S., Kelly,S., Kershaw,J.K., Khan,Z.,
            Kioschis,P., Klages,S., Knights,A.J., Kosiura,A., Kovar-Smith,C.,
            Laird,G.K., Langford,C., Lawlor,S., Leversha,M., Lewis,L., Liu,W.,
            Lloyd,C., Lloyd,D.M., Loulseged,H., Loveland,J.E., Lovell,J.D.,
            Lozado,R., Lu,J., Lyne,R., Ma,J., Maheshwari,M., Matthews,L.H.,
            McDowall,J., McLaren,S., McMurray,A., Meidl,P., Meitinger,T.,
            Milne,S., Miner,G., Mistry,S.L., Morgan,M., Morris,S., Muller,I.,
            Mullikin,J.C., Nguyen,N., Nordsiek,G., Nyakatura,G., O'Dell,C.N.,
            Okwuonu,G., Palmer,S., Pandian,R., Parker,D., Parrish,J.,
            Pasternak,S., Patel,D., Pearce,A.V., Pearson,D.M., Pelan,S.E.,
            Perez,L., Porter,K.M., Ramsey,Y., Reichwald,K., Rhodes,S.,
            Ridler,K.A., Schlessinger,D., Schueler,M.G., Sehra,H.K.,
            Shaw-Smith,C., Shen,H., Sheridan,E.M., Shownkeen,R., Skuce,C.D.,
            Smith,M.L., Sotheran,E.C., Steingruber,H.E., Steward,C.A.,
            Storey,R., Swann,R.M., Swarbreck,D., Tabor,P.E., Taudien,S.,
            Taylor,T., Teague,B., Thomas,K., Thorpe,A., Timms,K., Tracey,A.,
            Trevanion,S., Tromans,A.C., d'Urso,M., Verduzco,D., Villasana,D.,
            Waldron,L., Wall,M., Wang,Q., Warren,J., Warry,G.L., Wei,X.,
            West,A., Whitehead,S.L., Whiteley,M.N., Wilkinson,J.E.,
            Willey,D.L., Williams,G., Williams,L., Williamson,A.,
            Williamson,H., Wilming,L., Woodmansey,R.L., Wray,P.W., Yen,J.,
            Zhang,J., Zhou,J., Zoghbi,H., Zorilla,S., Buck,D., Reinhardt,R.,
            Poustka,A., Rosenthal,A., Lehrach,H., Meindl,A., Minx,P.J.,
            Hillier,L.W., Willard,H.F., Wilson,R.K., Waterston,R.H., Rice,C.M.,
            Vaudin,M., Coulson,A., Nelson,D.L., Weinstock,G., Sulston,J.E.,
            Durbin,R., Hubbard,T., Gibbs,R.A., Beck,S., Rogers,J. and
            Bentley,D.R.
  TITLE     The DNA sequence of the human X chromosome
  JOURNAL   Nature 434 (7031), 325-337 (2005)
   PUBMED   15772651
REFERENCE   8  (residues 1 to 1395)
  AUTHORS   Heydorn,A., Sondergaard,B.P., Ersboll,B., Holst,B., Nielsen,F.C.,
            Haft,C.R., Whistler,J. and Schwartz,T.W.
  TITLE     A library of 7TM receptor C-terminal tails. Interactions with the
            proposed post-endocytic sorting proteins ERM-binding phosphoprotein
            50 (EBP50), N-ethylmaleimide-sensitive factor (NSF), sorting nexin
            1 (SNX1), and G protein-coupled receptor-associated sorting protein
            (GASP)
  JOURNAL   J Biol Chem 279 (52), 54291-54303 (2004)
   PUBMED   15452121
REFERENCE   9  (residues 1 to 1395)
  AUTHORS   Simonin,F., Karcher,P., Boeuf,J.J., Matifas,A. and Kieffer,B.L.
  TITLE     Identification of a novel family of G protein-coupled receptor
            associated sorting proteins
  JOURNAL   J Neurochem 89 (3), 766-775 (2004)
   PUBMED   15086532
REFERENCE   10 (residues 1 to 1395)
  AUTHORS   Whistler,J.L., Enquist,J., Marley,A., Fong,J., Gladher,F.,
            Tsuruda,P., Murray,S.R. and Von Zastrow,M.
  TITLE     Modulation of postendocytic sorting of G protein-coupled receptors
  JOURNAL   Science 297 (5581), 615-620 (2002)
   PUBMED   12142540
  REMARK    GeneRIF: identified a protein that binds preferentially to the
            cytoplasmic tail of the delta opioid receptor as a candidate
            heterotrimeric GTP-binding protein (G protein)-coupled
            receptor-associated sorting protein (GASP) [GASP]
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BX954816.1, BX489726.1,
            BC114552.1, AY044233.1 and AW293629.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            Summary: This gene encodes a member of the GPRASP (G
            protein-coupled receptor associated sorting protein) family. The
            protein may modulate lysosomal sorting and functional
            down-regulation of a variety of G-protein coupled receptors. It
            targets receptors for degradation in lysosomes. The receptors
            interacting with this sorting protein include D2 dopamine receptor
            (DRD2), delta opioid receptor (OPRD1), beta-2 adrenergic receptor
            (ADRB2), D4 dopamine receptor (DRD4) and cannabinoid 1 receptor
            (CB1R). Multiple alternatively spliced transcript variants encoding
            the same protein have been identified. [provided by RefSeq, May
            2010].
            
            Transcript Variant: This variant (4) has an additional exon in the
            5' UTR and encodes the same protein, as compared to variant 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803612.256013.1,
                                           SRR1803613.156277.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968189, SAMEA1968540
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000537097.2/ ENSP00000445683.1
            RefSeq Select criteria :: based on expression, longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..1395
                     /organism="Homo sapiens"
                     /db_xref="taxon:9606"
                     /chromosome="X"
                     /map="Xq22.1"
     Protein         1..1395
                     /product="G-protein coupled receptor-associated sorting
                     protein 1"
                     /calculated_mol_wt=156735
     Region          1..25
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q5JY77.3)"
     Region          45..83
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q5JY77.3)"
     Region          269..288
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q5JY77.3)"
     Site            297
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q5U4C1; propagated from
                     UniProtKB/Swiss-Prot (Q5JY77.3)"
     Region          446..469
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q5JY77.3)"
     Site            631
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q5U4C1; propagated from
                     UniProtKB/Swiss-Prot (Q5JY77.3)"
     Region          899..1395
                     /region_name="OPRD1-binding"
                     /note="propagated from UniProtKB/Swiss-Prot (Q5JY77.3)"
     Site            899
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q920R4; propagated from
                     UniProtKB/Swiss-Prot (Q5JY77.3)"
     Region          1167..1370
                     /region_name="Arm_2"
                     /note="Armadillo-like; pfam04826"
                     /db_xref="CDD:461447"
     CDS             1..1395
                     /gene="GPRASP1"
                     /gene_synonym="GASP; GASP-1; GASP1"
                     /coded_by="NM_001184727.2:732..4919"
                     /db_xref="CCDS:CCDS35352.1"
                     /db_xref="GeneID:9737"
                     /db_xref="HGNC:HGNC:24834"
                     /db_xref="MIM:300417"
ORIGIN      
        1 mtgaeiesga qvkpekkpge evvggaeien dvplvvrpkv rtqaqimpga rpknkskvmp
       61 gastkvetsa vggarpkska kaipvsrfke eaqmwaqprf gaerlskter nsqtniiasp
      121 lvstdsvlva ktkylsedre lvntdtesfp rrkahyqagf qpsfrskeet nmgswccprp
      181 tskqeaspns dfkwvdksvs slfwsgdevt akfhpgnrvk dsnrsmhman qeantmsrsq
      241 tnqelyiass sgsedesvkt pwfwardktn twsgpredpn srsrfrskke vyvesssgse
      301 hedhleswfg agkeakfrsk mragkeannr arhrakreac idfmpgsidv ikkescfwpe
      361 enantfsrpm ikkeararam tkeeaktkar arakqearse eealigtwfw atdessmade
      421 asiesslqve desiigswfw teeeasmgtg assksrprtd gerigdslfg arektsmktg
      481 aeatsesila addeqviigs wfwageevnq eaeeetifgs wfwvidaasv esgvgvsces
      541 rtrseeeevi gpwfwsgeqv dieagigeea rpgaeeetif gswfwaenqt ymdcraetsc
      601 dtmqgaeeee piigswfwtr veacvegdvn skssledkee amipcfgake evsmkhgtgv
      661 rcrfmagaee tnnkscfwae kepcmypagg gswksrpeee edivnswfws rkytkpeaii
      721 gswlwatees nidgtgekak llteeetiin swfwkedeai seatdreesr peaeegdiig
      781 swfwageedr lepaaetree drlaaekegi vgswfgaree tirreagscs ksspkaeeee
      841 viigswfwee easpeavagv gfeskpgtee eeitvgswfw peeeasiqag sqaveemese
      901 teeetifgsw fwdgkevsee agpccvskpe ddeemivesw fwsrdkaike tgtvatcesk
      961 peneegaivg swfeaedevd nrtdngsncg srtladedea ivgswfwagd eahfesnpsp
     1021 vfraicrstc sveqepdpsr rpqsweevtv qfkpgpwgrv gfpsispfrf pkeaaslfce
     1081 mfggkprnmv lspegedqes llqpdqpspe fpfqydpsyr svqeirehlr akestepess
     1141 scnciqcelk igseefeell llmekirdpf iheiskiamg mrsasqftrd firdsgvvsl
     1201 ietllnypss rvrtsflenm irmappypnl niiqtyickv ceetlaysvd speqlsgirm
     1261 irhlttttdy htlvanymsg flsllatgna ktrfhvlkml lnlsenlfmt kellsaeavs
     1321 efiglfnree tndniqivla ifenignnik ketvfsdddf nieplisafh kvekfakelq
     1381 gktdnqndpe gdqen
//
