LOCUS       NM_001177676            3056 bp    mRNA    linear   PRI 24-SEP-2024
DEFINITION  Homo sapiens G protein-coupled receptor 68 (GPR68), transcript
            variant 1, mRNA.
ACCESSION   NM_001177676
VERSION     NM_001177676.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3056)
  AUTHORS   Yu,S., Liu,D., Yan,C., Yuan,C., Zhang,C. and Zheng,S.
  TITLE     A novel mutation in GPR68 causes hypomaturation amelogenesis
            imperfecta
  JOURNAL   Arch Oral Biol 164, 105991 (2024)
   PUBMED   38761453
  REMARK    GeneRIF: A novel mutation in GPR68 causes hypomaturation
            amelogenesis imperfecta.
REFERENCE   2  (bases 1 to 3056)
  AUTHORS   Pissas,K.P., Grunder,S. and Tian,Y.
  TITLE     Functional expression of the proton sensors ASIC1a, TMEM206, and
            OGR1 together with BKCa channels is associated with cell volume
            changes and cell death under strongly acidic conditions in DAOY
            medulloblastoma cells
  JOURNAL   Pflugers Arch 476 (6), 923-937 (2024)
   PUBMED   38627262
  REMARK    GeneRIF: Functional expression of the proton sensors ASIC1a,
            TMEM206, and OGR1 together with BKCa channels is associated with
            cell volume changes and cell death under strongly acidic conditions
            in DAOY medulloblastoma cells.
REFERENCE   3  (bases 1 to 3056)
  AUTHORS   Khan,N.M., Diaz-Hernandez,M.E., Martin,W.N., Patel,B., Chihab,S.
            and Drissi,H.
  TITLE     pH-sensing G protein-coupled orphan receptor GPR68 is expressed in
            human cartilage and correlates with degradation of extracellular
            matrix during OA progression
  JOURNAL   PeerJ 11, e16553 (2023)
   PUBMED   38077417
  REMARK    GeneRIF: pH-sensing G protein-coupled orphan receptor GPR68 is
            expressed in human cartilage and correlates with degradation of
            extracellular matrix during OA progression.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3056)
  AUTHORS   van Setten,G.B.
  TITLE     Expression of GPR-68 in Human Corneal and Conjunctival Epithelium.
            Possible indicator and mediator of attrition associated
            inflammation at the ocular surface
  JOURNAL   J Fr Ophtalmol 46 (1), 19-24 (2023)
   PUBMED   36503812
  REMARK    GeneRIF: Expression of GPR-68 in Human Corneal and Conjunctival
            Epithelium. Possible indicator and mediator of attrition associated
            inflammation at the ocular surface.
REFERENCE   5  (bases 1 to 3056)
  AUTHORS   van Setten,G.B.
  TITLE     GPR-68 in human lacrimal gland. Detection and possible role in the
            pathogenesis of dry eye disease
  JOURNAL   J Fr Ophtalmol 45 (8), 921-927 (2022)
   PUBMED   35623913
  REMARK    GeneRIF: GPR-68 in human lacrimal gland. Detection and possible
            role in the pathogenesis of dry eye disease.
REFERENCE   6  (bases 1 to 3056)
  AUTHORS   Bektas,M., Barak,L.S., Jolly,P.S., Liu,H., Lynch,K.R., Lacana,E.,
            Suhr,K.B., Milstien,S. and Spiegel,S.
  TITLE     The G protein-coupled receptor GPR4 suppresses ERK activation in a
            ligand-independent manner
  JOURNAL   Biochemistry 42 (42), 12181-12191 (2003)
   PUBMED   14567679
REFERENCE   7  (bases 1 to 3056)
  AUTHORS   Ludwig,M.G., Vanek,M., Guerini,D., Gasser,J.A., Jones,C.E.,
            Junker,U., Hofstetter,H., Wolf,R.M. and Seuwen,K.
  TITLE     Proton-sensing G-protein-coupled receptors
  JOURNAL   Nature 425 (6953), 93-98 (2003)
   PUBMED   12955148
  REMARK    GeneRIF: ovarian cancer G-protein-coupled receptor 1 (OGR1),
            previously described as a receptor for sphingosylphosphorylcholine,
            acts as a proton-sensing receptor stimulating inositol phosphate
            formation
REFERENCE   8  (bases 1 to 3056)
  AUTHORS   Xu,Y.
  TITLE     Sphingosylphosphorylcholine and lysophosphatidylcholine: G
            protein-coupled receptors and receptor-mediated signal transduction
  JOURNAL   Biochim Biophys Acta 1582 (1-3), 81-88 (2002)
   PUBMED   12069813
  REMARK    Review article
REFERENCE   9  (bases 1 to 3056)
  AUTHORS   Xu,Y. and Casey,G.
  TITLE     Identification of human OGR1, a novel G protein-coupled receptor
            that maps to chromosome 14
  JOURNAL   Genomics 35 (2), 397-402 (1996)
   PUBMED   8661159
REFERENCE   10 (bases 1 to 3056)
  AUTHORS   An,S., Tsai,C. and Goetzl,E.J.
  TITLE     Cloning, sequencing and tissue distribution of two related G
            protein-coupled receptor candidates expressed prominently in human
            lung tissue
  JOURNAL   FEBS Lett 375 (1-2), 121-124 (1995)
   PUBMED   7498459
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL135818.3, BC128536.1 and
            BC098567.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_001177676.1.
            
            Summary: The protein encoded by this gene is a G protein-coupled
            receptor for sphingosylphosphorylcholine. The encoded protein is a
            proton-sensing receptor, inactive at pH 7.8 but active at pH 6.8.
            Mutations in this gene are a cause of amelogenesis imperfecta.
            [provided by RefSeq, Feb 2017].
            
            Transcript Variant: This variant (1) differs in the 5' UTR compared
            to variant 3. Variants 1-3 all encode the same protein. There is an
            upstream in-frame AUG (uAUG) present; however, translation is
            thought to begin from the annotated downstream AUG due to
            inhibition of the uAUG by a small overlapping open reading frame.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803613.197127.1,
                                           SRR1660803.223864.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            CDS uses downstream in-frame AUG :: upstream ORF predicted to
                                                inhibit translation from
                                                upstream AUG
            MANE Ensembl match               :: ENST00000650645.1/
                                                ENSP00000498702.1
            RefSeq Select criteria           :: based on conservation,
                                                expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-196               AL135818.3         28569-28764         c
            197-1551            BC128536.1         9-1363
            1552-3056           BC098567.1         1253-2757
FEATURES             Location/Qualifiers
     source          1..3056
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="14"
                     /map="14q32.11"
     gene            1..3056
                     /gene="GPR68"
                     /gene_synonym="AI2A6; GPR12A; OGR1"
                     /note="G protein-coupled receptor 68"
                     /db_xref="GeneID:8111"
                     /db_xref="HGNC:HGNC:4519"
                     /db_xref="MIM:601404"
     exon            1..408
                     /gene="GPR68"
                     /gene_synonym="AI2A6; GPR12A; OGR1"
                     /inference="alignment:Splign:2.1.0"
     exon            409..3056
                     /gene="GPR68"
                     /gene_synonym="AI2A6; GPR12A; OGR1"
                     /inference="alignment:Splign:2.1.0"
     CDS             538..1635
                     /gene="GPR68"
                     /gene_synonym="AI2A6; GPR12A; OGR1"
                     /note="ovarian cancer G protein-coupled receptor, 1;
                     sphingosylphosphorylcholine receptor"
                     /codon_start=1
                     /product="ovarian cancer G-protein coupled receptor 1"
                     /protein_id="NP_001171147.1"
                     /db_xref="CCDS:CCDS9894.2"
                     /db_xref="GeneID:8111"
                     /db_xref="HGNC:HGNC:4519"
                     /db_xref="MIM:601404"
                     /translation="MGNITADNSSMSCTIDHTIHQTLAPVVYVTVLVVGFPANCLSLY
                     FGYLQIKARNELGVYLCNLTVADLFYICSLPFWLQYVLQHDNWSHGDLSCQVCGILLY
                     ENIYISVGFLCCISVDRYLAVAHPFRFHQFRTLKAAVGVSVVIWAKELLTSIYFLMHE
                     EVIEDENQHRVCFEHYPIQAWQRAINYYRFLVGFLFPICLLLASYQGILRAVRRSHGT
                     QKSRKDQIQRLVLSTVVIFLACFLPYHVLLLVRSVWEASCDFAKGVFNAYHFSLLLTS
                     FNCVADPVLYCFVSETTHRDLARLRGACLAFLTCSRTGRAREAYPLGAPEASGKSGAQ
                     GEEPELLTKLHPAFQTPNSPGSGGFPTGRLA"
     regulatory      3037..3042
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GPR68"
                     /gene_synonym="AI2A6; GPR12A; OGR1"
                     /note="hexamer: AATAAA"
     polyA_site      3056
                     /gene="GPR68"
                     /gene_synonym="AI2A6; GPR12A; OGR1"
                     /note="major polyA site"
ORIGIN      
        1 aggctgcggg aagtcgctgg agggagagct gggatcgtgg ctgcaagggt gcccaggctc
       61 ctgcagagcc tctgcaggaa gcccagctgt ttgaggtttg aaggcagagc ctagctgggg
      121 caggcagagt cagagaggac tcgtttcaga gtcccccctc agaggactcc ctccatcctg
      181 atttcagcag ctgcctgctc ctcacctggt tgcagagacc acactgcctt cctggggccc
      241 tgaggctcta ggagaagctg gaaagcacag gtgggggtcc atccagctgt ttccaaactg
      301 cgggttgtga cccattcatg ggtcagtaaa tcaacttcat agctcatgac ctgcatttaa
      361 aaaattgaaa tagaatagaa atgatcagag tgcgtggcaa gaagtagggg tcttgcccac
      421 caccagcgat gcccagccct tggtagagct tgaaccacct tctataaaca ggatggcggt
      481 ggagagacag gcccagtccc tgagcccatg aggagtgtgg ccccttcagg cccaaagatg
      541 gggaacatca ctgcagacaa ctcctcgatg agctgtacca tcgaccatac catccaccag
      601 acgctggccc cggtggtcta tgttaccgtg ctggtggtgg gcttcccggc caactgcctg
      661 tccctctact tcggctacct gcagatcaag gcccggaacg agctgggcgt gtacctgtgc
      721 aacctgacgg tggccgacct cttctacatc tgctcgctgc ccttctggct gcagtacgtg
      781 ctgcagcacg acaactggtc tcacggcgac ctgtcctgcc aggtgtgcgg catcctcctg
      841 tacgagaaca tctacatcag cgtgggcttc ctctgctgca tctccgtgga ccgctacctg
      901 gctgtggccc atcccttccg cttccaccag ttccggaccc tgaaggcggc cgtcggcgtc
      961 agcgtggtca tctgggccaa ggagctgctg accagcatct acttcctgat gcacgaggag
     1021 gtcatcgagg acgagaacca gcaccgcgtg tgctttgagc actaccccat ccaggcatgg
     1081 cagcgcgcca tcaactacta ccgcttcctg gtgggcttcc tcttccccat ctgcctgctg
     1141 ctggcgtcct accagggcat cctgcgcgcc gtgcgccgga gccacggcac ccagaagagc
     1201 cgcaaggacc agatccagcg gctggtgctc agcaccgtgg tcatcttcct ggcctgcttc
     1261 ctgccctacc acgtgttgct gctggtgcgc agcgtctggg aggccagctg cgacttcgcc
     1321 aagggcgttt tcaacgccta ccacttctcc ctcctgctca ccagcttcaa ctgcgtcgcc
     1381 gaccccgtgc tctactgctt cgtcagcgag accacccacc gggacctggc ccgcctccgc
     1441 ggggcctgcc tggccttcct cacctgctcc aggaccggcc gggccaggga ggcctacccg
     1501 ctgggtgccc ccgaggcctc cgggaaaagc ggggcccagg gtgaggagcc cgagctgttg
     1561 accaagctcc acccggcctt ccagacccct aactcgccag ggtcgggcgg gttccccacg
     1621 ggcaggttgg cctagcctgg gtcctccgcg ggtggctcca cgtgaggcct gagccttcag
     1681 cccacgggcc tcagggcctg ccgcctcctg cttccctcgc tgcggaggca gggaagcccc
     1741 tgtaactccg gaagcctgct ctcgcttgct gagcccgctg ggaccgccga gggtgggaat
     1801 aagccccggt tggctcgtgg gaataagccg tgtcctctgc cgcggctgcg atgtggccag
     1861 gctggggctg ctggtcgggg gaagacagtg aactgcgttc cctggcctgc ttcctgcaga
     1921 gtttgtgcat ggggagtgtg aggacatgga gggtgggagg ctgggcgttc agctgccagg
     1981 gccttccaat gccaccgttg tcacagacaa tgcctgtcca aacgtcccgg tgggatcagc
     2041 actgcagccc gcccacaaca gggtgggaag ggaagactgg agggggaagg aaggcaggag
     2101 ggggaaggaa gaaagagggg gaaggaaaga aagagggggg aaggaaagga ggaaggggag
     2161 aaggaaggga ggaggaaaga aggaaggtag ggggtgttgg gttgggagaa ttgagggagt
     2221 tataggcaga ggaggatcct agctccagcc ataagaactg ggagagccgc ctgctgctcc
     2281 agaagacctg cccacctcca cagaaatgct agcccttcag accttctggt cagtgaactg
     2341 ggaccagctc tgactaagga tgccttccat ggcctgggac tcgagttctt cagaaccaag
     2401 aggctaactg ggcttcctcc aagaagggac caggggctag aagcagaagt tggcatcagc
     2461 aaacatttct ggaatctacc aaagggaggc ccaggcagaa ccccttcctc agtctccctt
     2521 cccctcttcc ccccagcctt gaagaggttg atcctttgtg ctgtgtctct tagccctttc
     2581 atcggggatg cccagaggca acagcccagg ctggaggtgc cccagggaag ggtgctcaca
     2641 gtggtcccag ggctacactt ggctttgact gcacagcccc tgctcagatc cttgtggagg
     2701 gtgtccgatg acttggggag gttctcttcc ccttcctaga ggaggaagtg accaagtttg
     2761 aaggcggcag aaatggccac acagccaaga agagcccatg caactcagtc caggatttta
     2821 ctgggtcagt gacattggtg gaagccttca tgcctcctca ttccacagca cttcctccta
     2881 tgttgacctt aaacactggc ttcccactga tacagactgg gatgagctga gggcagtctc
     2941 atttactgtc aagagttgta tttttgtatt gtcctttttg ccaagtctac atgtgttgac
     3001 tctgtaatgg atttatgtag cccacttcag tctgcaaata aagcaaagta actgga
//
