LOCUS       NM_003608               4511 bp    mRNA    linear   PRI 11-FEB-2025
DEFINITION  Homo sapiens G protein-coupled receptor 65 (GPR65), mRNA.
ACCESSION   NM_003608
VERSION     NM_003608.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4511)
  AUTHORS   Caratis,F., Opielka,M., Hausmann,M., Velasco-Estevez,M., Rojek,B.,
            de Valliere,C., Seuwen,K., Rogler,G., Karaszewski,B. and
            Rutkowska,A.
  TITLE     The proton-sensing receptors TDAG8 and GPR4 are differentially
            expressed in human and mouse oligodendrocytes: Exploring their role
            in neuroinflammation and multiple sclerosis
  JOURNAL   PLoS One 19 (3), e0283060 (2024)
   PUBMED   38527054
  REMARK    GeneRIF: The proton-sensing receptors TDAG8 and GPR4 are
            differentially expressed in human and mouse oligodendrocytes:
            Exploring their role in neuroinflammation and multiple sclerosis.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4511)
  AUTHORS   Li,G., Lin,J., Gao,X., Su,H., Lin,R., Gao,H., Feng,Z., Wu,H.,
            Feng,B., Zuo,K., Li,Y., Wu,W., Fang,L. and Liu,Z.
  TITLE     Intestinal epithelial pH-sensing receptor GPR65 maintains mucosal
            homeostasis via regulating antimicrobial defense and restrains gut
            inflammation in inflammatory bowel disease
  JOURNAL   Gut Microbes 15 (2), 2257269 (2023)
   PUBMED   37749885
  REMARK    GeneRIF: Intestinal epithelial pH-sensing receptor GPR65 maintains
            mucosal homeostasis via regulating antimicrobial defense and
            restrains gut inflammation in inflammatory bowel disease.
REFERENCE   3  (bases 1 to 4511)
  AUTHORS   Zhang,K., Zhang,M.X., Meng,X.X., Zhu,J., Wang,J.J., He,Y.F.,
            Li,Y.H., Zhao,S.C., Shi,Z.M., Zheng,L.N., Han,T. and Hong,W.
  TITLE     Targeting GPR65 alleviates hepatic inflammation and fibrosis by
            suppressing the JNK and NF-kappaB pathways
  JOURNAL   Mil Med Res 10 (1), 56 (2023)
   PUBMED   38001521
  REMARK    GeneRIF: Targeting GPR65 alleviates hepatic inflammation and
            fibrosis by suppressing the JNK and NF-kappaB pathways.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4511)
  AUTHORS   Mao,J., Feng,Y., Zheng,Y., Gao,Y., Zhang,L., Sun,X., Wu,Y., Zhu,X.
            and Ma,F.
  TITLE     GPR65 inhibits human trophoblast cell adhesion through upregulation
            of MYLK and downregulation of fibronectin via cAMP-ERK signaling in
            a low pH environment
  JOURNAL   Cell Commun Signal 21 (1), 238 (2023)
   PUBMED   37723567
  REMARK    GeneRIF: GPR65 inhibits human trophoblast cell adhesion through
            upregulation of MYLK and downregulation of fibronectin via cAMP-ERK
            signaling in a low pH environment.
            Erratum:[Cell Commun Signal. 2024 Aug 27;22(1):414. doi:
            10.1186/s12964-024-01801-9.. PMID: 39192257]
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4511)
  AUTHORS   Xu,X., Bu,B., Tian,H., Wu,R. and Yang,J.
  TITLE     MicroRNAs combined with the TLR4/TDAG8 mRNAs and proinflammatory
            cytokines are biomarkers for the rapid diagnosis of sepsis
  JOURNAL   Mol Med Rep 26 (5) (2022)
   PUBMED   36102304
  REMARK    GeneRIF: MicroRNAs combined with the TLR4/TDAG8 mRNAs and
            proinflammatory cytokines are biomarkers for the rapid diagnosis of
            sepsis.
REFERENCE   6  (bases 1 to 4511)
  AUTHORS   Ishii,S., Kihara,Y. and Shimizu,T.
  TITLE     Identification of T cell death-associated gene 8 (TDAG8) as a novel
            acid sensing G-protein-coupled receptor
  JOURNAL   J Biol Chem 280 (10), 9083-9087 (2005)
   PUBMED   15618224
  REMARK    GeneRIF: TDAG8 may play biological roles in immune response and
            cellular transformation under conditions accompanying tissue
            acidosis.
REFERENCE   7  (bases 1 to 4511)
  AUTHORS   Wang,J.Q., Kon,J., Mogi,C., Tobo,M., Damirin,A., Sato,K.,
            Komachi,M., Malchinkhuu,E., Murata,N., Kimura,T., Kuwabara,A.,
            Wakamatsu,K., Koizumi,H., Uede,T., Tsujimoto,G., Kurose,H.,
            Sato,T., Harada,A., Misawa,N., Tomura,H. and Okajima,F.
  TITLE     TDAG8 is a proton-sensing and psychosine-sensitive
            G-protein-coupled receptor
  JOURNAL   J Biol Chem 279 (44), 45626-45633 (2004)
   PUBMED   15326175
  REMARK    GeneRIF: TDAG8 is one of the proton-sensing GPCRs coupling to
            adenylyl cyclase and psychosine, and its related lysosphingolipids
            behave as if they were antagonists against protein-sensing
            receptors, including TDAG8, GPR4, and OGR1.
REFERENCE   8  (bases 1 to 4511)
  AUTHORS   Sin,W.C., Zhang,Y., Zhong,W., Adhikarakunnathu,S., Powers,S.,
            Hoey,T., An,S. and Yang,J.
  TITLE     G protein-coupled receptors GPR4 and TDAG8 are oncogenic and
            overexpressed in human cancers
  JOURNAL   Oncogene 23 (37), 6299-6303 (2004)
   PUBMED   15221007
  REMARK    GeneRIF: GPR4 and TDAG8 overexpression in human tumors plays a role
            in driving or maintaining tumor formation
REFERENCE   9  (bases 1 to 4511)
  AUTHORS   Im,D.S., Heise,C.E., Nguyen,T., O'Dowd,B.F. and Lynch,K.R.
  TITLE     Identification of a molecular target of psychosine and its role in
            globoid cell formation
  JOURNAL   J Cell Biol 153 (2), 429-434 (2001)
   PUBMED   11309421
REFERENCE   10 (bases 1 to 4511)
  AUTHORS   Kyaw,H., Zeng,Z., Su,K., Fan,P., Shell,B.K., Carter,K.C. and Li,Y.
  TITLE     Cloning, characterization, and mapping of human homolog of mouse
            T-cell death-associated gene
  JOURNAL   DNA Cell Biol 17 (6), 493-500 (1998)
   PUBMED   9655242
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL157955.5.
            
            On Nov 22, 2018 this sequence version replaced NM_003608.3.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: DA950973.1, SRR1803615.13159.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000267549.5/ ENSP00000267549.3
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-88                AL157955.5         51766-51853
            89-4511             AL157955.5         57020-61442
FEATURES             Location/Qualifiers
     source          1..4511
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="14"
                     /map="14q31.3"
     gene            1..4511
                     /gene="GPR65"
                     /gene_synonym="hTDAG8; TDAG8"
                     /note="G protein-coupled receptor 65"
                     /db_xref="GeneID:8477"
                     /db_xref="HGNC:HGNC:4517"
                     /db_xref="MIM:604620"
     exon            1..88
                     /gene="GPR65"
                     /gene_synonym="hTDAG8; TDAG8"
                     /inference="alignment:Splign:2.1.0"
     exon            89..4511
                     /gene="GPR65"
                     /gene_synonym="hTDAG8; TDAG8"
                     /inference="alignment:Splign:2.1.0"
     CDS             548..1561
                     /gene="GPR65"
                     /gene_synonym="hTDAG8; TDAG8"
                     /note="T-cell death-associated gene 8 protein"
                     /codon_start=1
                     /product="psychosine receptor"
                     /protein_id="NP_003599.2"
                     /db_xref="CCDS:CCDS9879.1"
                     /db_xref="GeneID:8477"
                     /db_xref="HGNC:HGNC:4517"
                     /db_xref="MIM:604620"
                     /translation="MNSTCIEEQHDLDHYLFPIVYIFVIIVSIPANIGSLCVSFLQAK
                     KESELGIYLFSLSLSDLLYALTLPLWIDYTWNKDNWTFSPALCKGSAFLMYMNFYSST
                     AFLTCIAVDRYLAVVYPLKFFFLRTRRFALMVSLSIWILETIFNAVMLWEDETVVEYC
                     DAEKSNFTLCYDKYPLEKWQINLNLFRTCTGYAIPLVTILICNRKVYQAVRHNKATEN
                     KEKKRIIKLLVSITVTFVLCFTPFHVMLLIRCILEHAVNFEDHSNSGKRTYTMYRITV
                     ALTSLNCVADPILYCFVTETGRYDMWNILKFCTGRCNTSQRQRKRILSVSTKDTMELE
                     VLE"
     regulatory      1692..1697
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GPR65"
                     /gene_synonym="hTDAG8; TDAG8"
                     /note="hexamer: AATAAA"
     polyA_site      1714
                     /gene="GPR65"
                     /gene_synonym="hTDAG8; TDAG8"
                     /note="major polyA site"
     regulatory      1758..1763
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GPR65"
                     /gene_synonym="hTDAG8; TDAG8"
                     /note="hexamer: AACAAA"
     polyA_site      1780
                     /gene="GPR65"
                     /gene_synonym="hTDAG8; TDAG8"
     regulatory      4486..4491
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GPR65"
                     /gene_synonym="hTDAG8; TDAG8"
                     /note="hexamer: AATAAA"
     regulatory      4494..4499
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GPR65"
                     /gene_synonym="hTDAG8; TDAG8"
                     /note="hexamer: TATAAA"
     polyA_site      4507
                     /gene="GPR65"
                     /gene_synonym="hTDAG8; TDAG8"
     polyA_site      4511
                     /gene="GPR65"
                     /gene_synonym="hTDAG8; TDAG8"
ORIGIN      
        1 agcagtgttg gtttctcttc ttgacttgat gcaggcacag atttatcaag ctcctcagtc
       61 aacaaacaca tcaccggaag aaatatggaa ggaaaggaat tttaaaagga aataccaatc
      121 tctgtgcaaa caaagccttg tatattcatg tttgcaccaa tctactgtga gatttatgaa
      181 gaaaaacaaa ttgcggacaa ctctctatgt acacttacaa atgcctcagt tgatgcttgt
      241 gggctgtttg tcagcgttct gtgataatga acacatggac ttctgtttat taaattcagt
      301 tgaccccttt agccaattgc caggagcctg gatttttact tccaactgct gatatctgtg
      361 taaaaattga tctacatcca ccctttaaaa gcattgatga attaattaga actttagaca
      421 acaaagaaaa attgaaaaag aattctcagt aaaagcgaat tcgatgttca aaacaaacta
      481 caaagagaca agacttctct gtttactttc taagaactaa tataattgct accttaaaaa
      541 ggaaaaaatg aacagcacat gtattgaaga acagcatgac ctggatcact atttgtttcc
      601 cattgtttac atctttgtga ttatagtcag cattccagcc aatattggat ctctgtgtgt
      661 gtctttcctg caagcaaaga aggaaagtga actaggaatt tacctcttca gtttgtcact
      721 atcagattta ctctatgcat taactctccc tttatggatt gattatacct ggaataaaga
      781 caactggact ttctctcctg ccttgtgcaa agggagtgct tttctcatgt acatgaattt
      841 ttacagcagc acagcattcc tcacctgcat tgccgttgat cggtatttgg ctgttgtcta
      901 ccctttgaag ttttttttcc taaggacaag aagatttgca ctcatggtca gcctgtccat
      961 ctggatattg gaaaccatct tcaatgctgt catgttgtgg gaagatgaaa cagttgttga
     1021 atattgcgat gccgaaaagt ctaattttac tttatgctat gacaaatacc ctttagagaa
     1081 atggcaaatc aacctcaact tgttcaggac gtgtacaggc tatgcaatac ctttggtcac
     1141 catcctgatc tgcaaccgga aagtctacca agctgtgcgg cacaataaag ccacggaaaa
     1201 caaggaaaag aagagaatca taaaactact tgtcagcatc acagttactt ttgtcttatg
     1261 ctttactccc tttcatgtga tgttgctgat tcgctgcatt ttagagcatg ctgtgaactt
     1321 cgaagaccac agcaattctg ggaagcgaac ttacacaatg tatagaatca cggttgcatt
     1381 aacaagttta aattgtgttg ctgatccaat tctgtactgt tttgtaaccg aaacaggaag
     1441 atatgatatg tggaatatat taaaattctg cactgggagg tgtaatacat cacaaagaca
     1501 aagaaaacgc atactttctg tgtctacaaa agatactatg gaattagagg tccttgagta
     1561 gaaccaagga tgttttgaag ggaagggaag tttaagttat gcattattat atcatcaaga
     1621 ttacattttg aaaaggaaat ctagcatgtg aggggactaa gtgttctcag agtgatgttt
     1681 taatccagtc caataaaaat atcttaaaac tgcattgtac agctccctcc ctgcgtttta
     1741 ttaaatgatg tatattaaac aaagatcaat attttcttaa tgactcaggg tctttattgt
     1801 taatgccaat tgtttttgta tctgtgctat aatcccttag agtcagtaaa gtatgtaggg
     1861 gactgtttct tcctttgtgt ctgggtttat gatttttctc actctttctt tggactccag
     1921 ggtgtcagcc atcaggtctc ctaattttgt gtaccggtct ccaacaaccc cagctactga
     1981 atactgcttc taatctcctc attcattaac aaatctttat ttttttatct tgtataaaat
     2041 aactgcttta ttgacacaaa atttacataa cttaaaattc aactttgtat tgtgtacaat
     2101 tcagtgattt tttgtatatt cacagagctg tgcaaccatc accacactca aaaaattttc
     2161 atcacccacc aaagaaatct tatactctta gcagtcgctc cctgctctcc cgtccatgcc
     2221 agttattaat ttactttctg tctctaagga ttttcattac tctgaacatt tcatataaat
     2281 agaattatac aatatgtggc ctactgtgac gtatttcact tagtataatg gtttcaagtt
     2341 ttatccatgt gtagaatgta tcagcacttc atttcttttt atggcctgat agtattctgt
     2401 tgcatggtta tactccattt tgtttatcta atcacttggc ttcattaaca aatatttatt
     2461 gaatccattc cataaactag gttttgagtt aagtactggg gctatgaaag aaatggtctc
     2521 atgaagcctc acgaagttta cattagttca aaagcctagt caccgagctt gaaagatttc
     2581 tatataaagg aaaaggaaat aggctctgag ttttattttg atctcttttt aatttataac
     2641 tgggtataac atagctgaaa ttaccagaag tttaatgcat agacaaataa atagttctat
     2701 tatatctttc tttttggact tagaatgtta gaatattttg agagttcttt tttttttttt
     2761 ttttgagtca gagtcttgct ctgtaatcca ggctagagtg tagtggtgcg atctccactc
     2821 actgcagcct ccacctccca ggttcaagcg attctcctgc ctcagcctcc caagtagctg
     2881 ggattacagg cacccaccac catgcccagc taatttttgt atttttagta gagacggggt
     2941 ttcaccatgt tgcacaggct ggtctcaatc gaactcctga cctcaagtga tcatcccacc
     3001 taggtctccc aaagtgctga gatgacaggc gtgagccacc atgcctggca aagagagtct
     3061 tgatacaaca tattcttttg aatcctcatt gtgtaaattg cctcgttgta aatagacact
     3121 cagtaaacat tttcctcacc aaaatatttt taaggatttt tctacccttc tccttttctc
     3181 tttgctttcc ttttcttgcc tgttctttcc actcccccca aaatgatcag atagcaaatg
     3241 tcttgataac atgaggtgcc ctcacattaa aaaacaaaat attgagccgg gcgcggtggc
     3301 tcatgcctgt aatcccagca ctttgggagg ctgaggtggg cagatcgcct taggtcagga
     3361 gttggagacc aggctgacca atatgatgaa actctgtctc tactaaaaat tcaaaaatgt
     3421 gccagacctg gcctggtggc atgtgcctgt aatcccagct acttgggagg ctgagtcata
     3481 agcctgcaat gggaaaatgg atcgaatctg gggtgagggg gaagtgatgt gggggttatg
     3541 gtacctcttt tctcttccaa agatgctgtt cttactgcat cacttgtggc tggccaggaa
     3601 aagccatgca ggagttttgt ttgtggccac taggtgacga tcgtgttctg tacgggacct
     3661 cttattaata gttcaccact agccgccact ccagaagagc ggaggaaccc aggataatat
     3721 tttgtcaacc aagaaacaag aagtccctcc caggaactgg aaatgaatgg ggaaaatgct
     3781 gaaatctcat ttgcactatt catttctctt ctctctggaa agctcggcaa tcatcaggtc
     3841 atttcatttg gcttaaattc catgtgtctt tccaaacttt taaaagctgg tgaaaattgt
     3901 tccacccata tgtaaaagaa cataggttaa gttgtctaat tcttgcagga atgtggatat
     3961 agcattaaaa atatgtcttt gtatacttat cttacccatg taagaaaaga gtggccaact
     4021 ttcatataaa tagaaagaga acatttaagc tatatgcagt ttgcattttt gtctactatt
     4081 atgaaattat tatctatgaa attcaagctg taactcaaca tatgtataat tttaatttct
     4141 aatttattgt tagatctcag cacttaaaaa attacatctt gtatttgaat tgttaaatct
     4201 gttccctgca aagaacagta atacaatcat gttctaattt actagcattt gcatatttta
     4261 gaaatataat ggcctgtaat ttacttttct tttgcctata attttctgaa gctctttatg
     4321 atgcaccggt gcatttttat ttaaaaaata gattgtgact cctcaaataa tgttacaatt
     4381 cgatgttcaa aaagcaatcc aggtacatag ccataaaggg atgagctaga gaggtctcca
     4441 tattatcatt caatgtgaga ataaaaattc tatattttat tctagaataa aattataaat
     4501 ttctttatct a
//
