LOCUS       NM_005282               3052 bp    mRNA    linear   PRI 11-JUN-2024
DEFINITION  Homo sapiens G protein-coupled receptor 4 (GPR4), mRNA.
ACCESSION   NM_005282
VERSION     NM_005282.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3052)
  AUTHORS   Nakanishi,M., Ibe,A., Morishita,K., Shinagawa,K., Yamamoto,Y.,
            Takahashi,H., Ikemori,K., Muragaki,Y. and Ehata,S.
  TITLE     Acid-sensing receptor GPR4 plays a crucial role in lymphatic cancer
            metastasis
  JOURNAL   Cancer Sci 115 (5), 1551-1563 (2024)
   PUBMED   38410865
  REMARK    GeneRIF: Acid-sensing receptor GPR4 plays a crucial role in
            lymphatic cancer metastasis.
REFERENCE   2  (bases 1 to 3052)
  AUTHORS   Caratis,F., Opielka,M., Hausmann,M., Velasco-Estevez,M., Rojek,B.,
            de Valliere,C., Seuwen,K., Rogler,G., Karaszewski,B. and
            Rutkowska,A.
  TITLE     The proton-sensing receptors TDAG8 and GPR4 are differentially
            expressed in human and mouse oligodendrocytes: Exploring their role
            in neuroinflammation and multiple sclerosis
  JOURNAL   PLoS One 19 (3), e0283060 (2024)
   PUBMED   38527054
  REMARK    GeneRIF: The proton-sensing receptors TDAG8 and GPR4 are
            differentially expressed in human and mouse oligodendrocytes:
            Exploring their role in neuroinflammation and multiple sclerosis.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3052)
  AUTHORS   Stolwijk,J.A., Wallner,S., Heider,J., Kurz,B., Putz,L.,
            Michaelis,S., Goricnik,B., Erl,J., Frank,L., Berneburg,M.,
            Haubner,F., Wegener,J. and Schreml,S.
  TITLE     GPR4 in the pH-dependent migration of melanoma cells in the tumor
            microenvironment
  JOURNAL   Exp Dermatol 32 (4), 479-490 (2023)
   PUBMED   36562556
  REMARK    GeneRIF: GPR4 in the pH-dependent migration of melanoma cells in
            the tumor microenvironment.
REFERENCE   4  (bases 1 to 3052)
  AUTHORS   Rowe,J.B., Kapolka,N.J., Taghon,G.J., Morgan,W.M. and Isom,D.G.
  TITLE     The evolution and mechanism of GPCR proton sensing
  JOURNAL   J Biol Chem 296, 100167 (2021)
   PUBMED   33478938
  REMARK    GeneRIF: The evolution and mechanism of GPCR proton sensing.
REFERENCE   5  (bases 1 to 3052)
  AUTHORS   Qi,H., Yao,C., Xing,J. and Qin,Y.
  TITLE     Hypoxia-induced GPR4 suppresses trophoblast cell migration and
            proliferation through the MAPK signaling pathway
  JOURNAL   Reprod Toxicol 99, 1-8 (2021)
   PUBMED   33161135
  REMARK    GeneRIF: Hypoxia-induced GPR4 suppresses trophoblast cell migration
            and proliferation through the MAPK signaling pathway.
REFERENCE   6  (bases 1 to 3052)
  AUTHORS   Lum,H., Qiao,J., Walter,R.J., Huang,F., Subbaiah,P.V., Kim,K.S. and
            Holian,O.
  TITLE     Inflammatory stress increases receptor for lysophosphatidylcholine
            in human microvascular endothelial cells
  JOURNAL   Am J Physiol Heart Circ Physiol 285 (4), H1786-H1789 (2003)
   PUBMED   12805023
  REMARK    GeneRIF: Endogenous GPR4 in endothelial cells may be a potential G
            protein-coupled receptor by which LPC signals proinflammatory
            activities.
REFERENCE   7  (bases 1 to 3052)
  AUTHORS   Ludwig,M.G., Vanek,M., Guerini,D., Gasser,J.A., Jones,C.E.,
            Junker,U., Hofstetter,H., Wolf,R.M. and Seuwen,K.
  TITLE     Proton-sensing G-protein-coupled receptors
  JOURNAL   Nature 425 (6953), 93-98 (2003)
   PUBMED   12955148
  REMARK    GeneRIF: GPR4, a close relative of OGR1, also responds to pH
            changes, but elicits cyclic AMP formation
REFERENCE   8  (bases 1 to 3052)
  AUTHORS   An,S., Tsai,C. and Goetzl,E.J.
  TITLE     Cloning, sequencing and tissue distribution of two related G
            protein-coupled receptor candidates expressed prominently in human
            lung tissue
  JOURNAL   FEBS Lett 375 (1-2), 121-124 (1995)
   PUBMED   7498459
REFERENCE   9  (bases 1 to 3052)
  AUTHORS   Mahadevan,M.S., Baird,S., Bailly,J.E., Shutler,G.G., Sabourin,L.A.,
            Tsilfidis,C., Neville,C.E., Narang,M. and Korneluk,R.G.
  TITLE     Isolation of a novel G protein-coupled receptor (GPR4) localized to
            chromosome 19q13.3
  JOURNAL   Genomics 30 (1), 84-88 (1995)
   PUBMED   8595909
REFERENCE   10 (bases 1 to 3052)
  AUTHORS   Heiber,M., Docherty,J.M., Shah,G., Nguyen,T., Cheng,R., Heng,H.H.,
            Marchese,A., Tsui,L.C., Shi,X., George,S.R. et al.
  TITLE     Isolation of three novel human genes encoding G protein-coupled
            receptors
  JOURNAL   DNA Cell Biol 14 (1), 25-35 (1995)
   PUBMED   7832990
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC011480.3, DA858082.1, BC038450.1 and BE669939.1.
            
            On Nov 23, 2018 this sequence version replaced NM_005282.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC038450.1, SRR1660809.224954.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000323040.5/ ENSP00000319744.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-4                 AC011480.3         36602-36605
            5-551               DA858082.1         1-547
            552-2629            BC038450.1         536-2613
            2630-3052           BE669939.1         10-432              c
FEATURES             Location/Qualifiers
     source          1..3052
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="19"
                     /map="19q13.32"
     gene            1..3052
                     /gene="GPR4"
                     /gene_synonym="GPR6C.l"
                     /note="G protein-coupled receptor 4"
                     /db_xref="GeneID:2828"
                     /db_xref="HGNC:HGNC:4497"
                     /db_xref="MIM:600551"
     exon            1..118
                     /gene="GPR4"
                     /gene_synonym="GPR6C.l"
                     /inference="alignment:Splign:2.1.0"
     exon            119..3052
                     /gene="GPR4"
                     /gene_synonym="GPR6C.l"
                     /inference="alignment:Splign:2.1.0"
     CDS             950..2038
                     /gene="GPR4"
                     /gene_synonym="GPR6C.l"
                     /note="G-protein coupled receptor 19; G-protein coupled
                     receptor 6C.l"
                     /codon_start=1
                     /product="G-protein coupled receptor 4"
                     /protein_id="NP_005273.1"
                     /db_xref="CCDS:CCDS12669.1"
                     /db_xref="GeneID:2828"
                     /db_xref="HGNC:HGNC:4497"
                     /db_xref="MIM:600551"
                     /translation="MGNHTWEGCHVDSRVDHLFPPSLYIFVIGVGLPTNCLALWAAYR
                     QVQQRNELGVYLMNLSIADLLYICTLPLWVDYFLHHDNWIHGPGSCKLFGFIFYTNIY
                     ISIAFLCCISVDRYLAVAHPLRFARLRRVKTAVAVSSVVWATELGANSAPLFHDELFR
                     DRYNHTFCFEKFPMEGWVAWMNLYRVFVGFLFPWALMLLSYRGILRAVRGSVSTERQE
                     KAKIKRLALSLIAIVLVCFAPYHVLLLSRSAIYLGRPWDCGFEERVFSAYHSSLAFTS
                     LNCVADPILYCLVNEGARSDVAKALHNLLRFLASDKPQEMANASLTLETPLTSKRNST
                     AKAMTGSWAATPPSQGDQVQLKMLPPAQ"
     regulatory      2150..2155
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GPR4"
                     /gene_synonym="GPR6C.l"
                     /note="hexamer: AATACA"
     polyA_site      2173
                     /gene="GPR4"
                     /gene_synonym="GPR6C.l"
                     /note="major polyA site"
     regulatory      3020..3025
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GPR4"
                     /gene_synonym="GPR6C.l"
                     /note="hexamer: AATAAA"
     polyA_site      3052
                     /gene="GPR4"
                     /gene_synonym="GPR6C.l"
                     /note="major polyA site"
ORIGIN      
        1 agccgctgtt ctgccccgcg ggggcatccg ctgggtcccc gcgccgttgt caagaccggg
       61 gacctggacg cagcggagag aggtgccgcc gcccgccccg tccagtcgcc gcgcgcaggc
      121 actgcagtca gcggtgaact gacttcatcc caatccctca gcccccacca ggaccagtct
      181 ggagtccctc ccctgccccc attgaaattt cccttccgtc cccaaactta cctctgatct
      241 agaccttact cacctccttc ctgtttccta agactccttc ctgccgtcca cagaccgagc
      301 cttttatctt tgtccaccct gtgccagaca cctccttttc cagaaccttc tccttactgg
      361 tgaccttact tatctctgtt gctttctggg gtcctaggaa atgccagcac tcccacccac
      421 attgcctgaa ctttccaaca ctccctaact gcgctgtgtc ctatctcaac actttctcat
      481 gtatttcttg tgtcttctag aacattcccc cgccattatt acttcaatat ggctacacat
      541 acttcctaat tgccctgcaa accatctcct tctcaccatt gcccagcgat gctttcgtct
      601 cctccataaa cactcccgga gaccaatttt tgtgtcaccc ccatactccc tcgttgacac
      661 actgactcca tacataacct ccttgaaaaa cctctttatt aatctcacca tcctccagac
      721 ttccctcctg tcataattcc atccctcctc caacttttcc ctctcaagct ctgcccttcc
      781 cagcccagcc cagcctaccc aacctcatct cttccctgta gaccacatcc caccatgttc
      841 ccctgagcct ccaaggaagg ggctcagggg gccccatggc ctcccgctcc ctgtggcccc
      901 acagcccccg tgggccaggg gaagcgcccc agaagccgaa gtgcccacca tgggcaacca
      961 cacgtgggag ggctgccacg tggactcgcg cgtggaccac ctctttccgc catccctcta
     1021 catctttgtc atcggcgtgg ggctgcccac caactgcctg gctctgtggg cggcctaccg
     1081 ccaggtgcaa cagcgcaacg agctgggcgt ctacctgatg aacctcagca tcgccgacct
     1141 gctgtacatc tgcacgctgc cgctgtgggt ggactacttc ctgcaccacg acaactggat
     1201 ccacggcccc gggtcctgca agctctttgg gttcatcttc tacaccaata tctacatcag
     1261 catcgccttc ctgtgctgca tctcggtgga ccgctacctg gctgtggccc acccactccg
     1321 cttcgcccgc ctgcgccgcg tcaagaccgc cgtggccgtg agctccgtgg tctgggccac
     1381 ggagctgggc gccaactcgg cgcccctgtt ccatgacgag ctcttccgag accgctacaa
     1441 ccacaccttc tgctttgaga agttccccat ggaaggctgg gtggcctgga tgaacctcta
     1501 tcgggtgttc gtgggcttcc tcttcccgtg ggcgctcatg ctgctgtcgt accggggcat
     1561 cctgcgggcc gtgcggggca gcgtgtccac cgagcgccag gagaaggcca agatcaagcg
     1621 gctggccctc agcctcatcg ccatcgtgct ggtctgcttt gcgccctatc acgtgctctt
     1681 gctgtcccgc agcgccatct acctgggccg cccctgggac tgcggcttcg aggagcgcgt
     1741 cttttctgca taccacagct cactggcttt caccagcctc aactgtgtgg cggaccccat
     1801 cctctactgc ctggtcaacg agggcgcccg cagcgatgtg gccaaggccc tgcacaacct
     1861 gctccgcttt ctggccagcg acaagcccca ggagatggcc aatgcctcgc tcaccctgga
     1921 gaccccactc acctccaaga ggaacagcac agccaaagcc atgactggca gctgggcggc
     1981 cactccgccc tcccaggggg accaggtgca gctgaagatg ctgccgccag cacaatgaac
     2041 cccgagtggc acagaatccc cagttttccc ctctcatccc acagtccctt ctctcctggt
     2101 ctggtgtatg caaattgtat ggaaaaaggg ctgtgttaat attcataaga atacaagaac
     2161 ttaggaagag tgaggttggt gtgtcactgg tcaacctttg tgctcccaga tcccatcaca
     2221 gtttggcgat tgtggagggc ctcctgaagg aggagatgag taaatatatt tttttggaga
     2281 cagggtctca ctgtgttgcc caggctggag tgcagtagtg cagtcgtggc tcactgcagc
     2341 ctccacctcc tgggctctcc agcgatcttc ccacatcagc ctcccgagta gctgggacca
     2401 caaatgtgag ccaccatgcc tggctaattt ttgtactttt tgtagaaatg gagtctcact
     2461 atgtttccca ggctgatctt gaactcctgg gctcaagaga tcctcctgcc ttggcctccc
     2521 aaagtgctca gattagagat gtgagccgcc atgtctggcc agataaatta agtcaaacat
     2581 ttggtttcca gaaaataaag acaaatagag aaggttagat tttttttttt ccaacaagtg
     2641 gataaaagtc tgtgactcgg gggaaagtgg aaggagaaat gcagccgata tagagtcatt
     2701 atgtttgcaa agcccctggt catacaggcc agggaacata agaccgcaat tctaagtttc
     2761 tagataaaca gcgatctcca agtcaagact gaggatgaag agggagaatg tcagaactca
     2821 agtgaagggc aatcagggca gactgcctgg aggagtgatg ccagaaggtt tgggaagaag
     2881 gtgtgggaca agaagaaagg gtatttattc attcattcaa cagaggttta tgtagggcac
     2941 tgtgctgggt ggggctgggg acacaacaat gactgaggca gcctggcctt gccttcacag
     3001 ggctcaccat acacaagtaa ataaaaaata tgtaatgttt ggaattgcta aa
//
