LOCUS       NM_001098200            1394 bp    mRNA    linear   PRI 10-JUN-2024
DEFINITION  Homo sapiens G protein-coupled receptor 18 (GPR18), transcript
            variant 2, mRNA.
ACCESSION   NM_001098200
VERSION     NM_001098200.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1394)
  AUTHORS   Bardin,M., Pawelzik,S.C., Lagrange,J., Mahdi,A., Arnardottir,H.,
            Regnault,V., Feve,B., Lacolley,P., Michel,J.B., Mercier,N. and
            Back,M.
  TITLE     The resolvin D2 - GPR18 axis is expressed in human coronary
            atherosclerosis and transduces atheroprotection in apolipoprotein E
            deficient mice
  JOURNAL   Biochem Pharmacol 201, 115075 (2022)
   PUBMED   35525326
  REMARK    GeneRIF: The resolvin D2 - GPR18 axis is expressed in human
            coronary atherosclerosis and transduces atheroprotection in
            apolipoprotein E deficient mice.
REFERENCE   2  (bases 1 to 1394)
  AUTHORS   Fabisiak,A., Fabisiak,N., Mokrowiecka,A., Malecka-Panas,E.,
            Jacenik,D., Kordek,R., Zielinska,M., Kiec-Kononowicz,K. and
            Fichna,J.
  TITLE     Novel selective agonist of GPR18, PSB-KK-1415 exerts potent
            anti-inflammatory and anti-nociceptive activities in animal models
            of intestinal inflammation and inflammatory pain
  JOURNAL   Neurogastroenterol Motil 33 (3), e14003 (2021)
   PUBMED   33058313
  REMARK    GeneRIF: Novel selective agonist of GPR18, PSB-KK-1415 exerts
            potent anti-inflammatory and anti-nociceptive activities in animal
            models of intestinal inflammation and inflammatory pain.
REFERENCE   3  (bases 1 to 1394)
  AUTHORS   Sotudeh,N., Morales,P., Hurst,D.P., Lynch,D.L. and Reggio,P.H.
  TITLE     Towards A Molecular Understanding of The Cannabinoid Related Orphan
            Receptor GPR18: A Focus on Its Constitutive Activity
  JOURNAL   Int J Mol Sci 20 (9), 2300 (2019)
   PUBMED   31075933
  REMARK    GeneRIF: molecular dynamics simulations of wild-type (WT) GPR18 and
            the A3.39N mutant in fully hydrated (POPC) phophatidylcholine lipid
            bilayers, are reported.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1394)
  AUTHORS   Zhang,L., Qiu,C., Yang,L., Zhang,Z., Zhang,Q., Wang,B. and Wang,X.
  TITLE     GPR18 expression on PMNs as biomarker for outcome in patient with
            sepsis
  JOURNAL   Life Sci 217, 49-56 (2019)
   PUBMED   30503722
  REMARK    GeneRIF: Lipopolysaccharides-Toll-like receptor (TLR)4 signaling
            mediated the GPR18 expression on polymorphonuclear neutrophils.
            These results indicate that decreased percentage GPR18-positive
            polymorphonuclear neutrophils is associated with increased severity
            and poorer outcome of sepsis.
REFERENCE   5  (bases 1 to 1394)
  AUTHORS   Pirault,J. and Back,M.
  TITLE     Lipoxin and Resolvin Receptors Transducing the Resolution of
            Inflammation in Cardiovascular Disease
  JOURNAL   Front Pharmacol 9, 1273 (2018)
   PUBMED   30487747
  REMARK    Review article
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1394)
  AUTHORS   Qin,Y., Verdegaal,E.M., Siderius,M., Bebelman,J.P., Smit,M.J.,
            Leurs,R., Willemze,R., Tensen,C.P. and Osanto,S.
  TITLE     Quantitative expression profiling of G-protein-coupled receptors
            (GPCRs) in metastatic melanoma: the constitutively active orphan
            GPCR GPR18 as novel drug target
  JOURNAL   Pigment Cell Melanoma Res 24 (1), 207-218 (2011)
   PUBMED   20880198
  REMARK    GeneRIF: GPR18, the most abundantly overexpressed orphan
            G-protein-coupled receptor in all melanoma metastases, is
            constitutively active and inhibits apoptosis, indicating an
            important role for GPR18 in tumor cell survival.
REFERENCE   7  (bases 1 to 1394)
  AUTHORS   Lambert,N.A.
  TITLE     Dissociation of heterotrimeric g proteins in cells
  JOURNAL   Sci Signal 1 (25), re5 (2008)
   PUBMED   18577758
  REMARK    Review article
            Erratum:[Sci Signal. 2008 Jul 15;1(28):er3]
            Publication Status: Online-Only
REFERENCE   8  (bases 1 to 1394)
  AUTHORS   Kohno,M., Hasegawa,H., Inoue,A., Muraoka,M., Miyazaki,T., Oka,K.
            and Yasukawa,M.
  TITLE     Identification of N-arachidonylglycine as the endogenous ligand for
            orphan G-protein-coupled receptor GPR18
  JOURNAL   Biochem Biophys Res Commun 347 (3), 827-832 (2006)
   PUBMED   16844083
  REMARK    GeneRIF: N-arachidonylglycine is a natural ligand for GPR18
REFERENCE   9  (bases 1 to 1394)
  AUTHORS   Christian,S.L., McDonough,J., Liu Cy,C.Y., Shaikh,S., Vlamakis,V.,
            Badner,J.A., Chakravarti,A. and Gershon,E.S.
  TITLE     An evaluation of the assembly of an approximately 15-Mb region on
            human chromosome 13q32-q33 linked to bipolar disorder and
            schizophrenia
  JOURNAL   Genomics 79 (5), 635-656 (2002)
   PUBMED   11991713
REFERENCE   10 (bases 1 to 1394)
  AUTHORS   Gantz,I., Muraoka,A., Yang,Y.K., Samuelson,L.C., Zimmerman,E.M.,
            Cook,H. and Yamada,T.
  TITLE     Cloning and chromosomal localization of a gene (GPR18) encoding a
            novel seven transmembrane receptor highly expressed in spleen and
            testis
  JOURNAL   Genomics 42 (3), 462-466 (1997)
   PUBMED   9205118
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BC008569.1 and AA825595.1.
            
            On Aug 13, 2020 this sequence version replaced NM_001098200.1.
            
            Transcript Variant: This variant (2) differs in the 5' UTR,
            compared to variant 1. Variants 1 and 2 encode the same protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF339781.1, AF261135.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000397470.5/ ENSP00000380610.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1390              BC008569.1         9-1398
            1391-1394           AA825595.1         42-45               c
FEATURES             Location/Qualifiers
     source          1..1394
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="13"
                     /map="13q32.3"
     gene            1..1394
                     /gene="GPR18"
                     /gene_synonym="DRV2"
                     /note="G protein-coupled receptor 18"
                     /db_xref="GeneID:2841"
                     /db_xref="HGNC:HGNC:4472"
                     /db_xref="MIM:602042"
     exon            1..226
                     /gene="GPR18"
                     /gene_synonym="DRV2"
                     /inference="alignment:Splign:2.1.0"
     exon            227..1394
                     /gene="GPR18"
                     /gene_synonym="DRV2"
                     /inference="alignment:Splign:2.1.0"
     CDS             261..1256
                     /gene="GPR18"
                     /gene_synonym="DRV2"
                     /note="NAGly receptor; resolvin D2 receptor"
                     /codon_start=1
                     /product="N-arachidonyl glycine receptor"
                     /protein_id="NP_001091670.1"
                     /db_xref="CCDS:CCDS9491.1"
                     /db_xref="GeneID:2841"
                     /db_xref="HGNC:HGNC:4472"
                     /db_xref="MIM:602042"
                     /translation="MITLNNQDQPVPFNSSHPDEYKIAALVFYSCIFIIGLFVNITAL
                     WVFSCTTKKRTTVTIYMMNVALVDLIFIMTLPFRMFYYAKDEWPFGEYFCQILGALTV
                     FYPSIALWLLAFISADRYMAIVQPKYAKELKNTCKAVLACVGVWIMTLTTTTPLLLLY
                     KDPDKDSTPATCLKISDIIYLKAVNVLNLTRLTFFFLIPLFIMIGCYLVIIHNLLHGR
                     TSKLKPKVKEKSIRIIITLLVQVLVCFMPFHICFAFLMLGTGENSYNPWGAFTTFLMN
                     LSTCLDVILYYIVSKQFQARVISVMLYRNYLRSMRRKSFRSGSLRSLSNINSEML"
     regulatory      1363..1368
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GPR18"
                     /gene_synonym="DRV2"
                     /note="hexamer: AATAAA"
     polyA_site      1394
                     /gene="GPR18"
                     /gene_synonym="DRV2"
                     /note="major polyA site"
ORIGIN      
        1 caaagcagca attaaagtca gcccagcacc aactccgacg ccaagcgtta cactggaaac
       61 tactttttaa agcaacaaaa gagtctaaaa caaaatacaa catttcttaa atacactgtt
      121 tccagaaaga gctattttaa cagaagcaac tcaaagatat cccttcgaca gaagtggaag
      181 tgctgaaaaa tgctcatctc tcacacagac ttttgatgga caggagtttc taagtatcat
      241 gcctaccaac aagctgtaaa atgatcaccc tgaacaatca agatcaacct gtccctttta
      301 acagctcaca tccagatgaa tacaaaattg cagcccttgt cttctatagc tgtatcttca
      361 taattggatt atttgttaac atcactgcat tatgggtttt cagttgtacc accaagaaga
      421 gaaccacggt aaccatctat atgatgaatg tggcattagt ggacttgata tttataatga
      481 ctttaccctt tcgaatgttt tattatgcaa aagatgaatg gccatttgga gagtacttct
      541 gccagattct tggagctctc acagtgtttt acccaagcat tgctttatgg cttcttgcct
      601 ttattagtgc tgacagatac atggccattg tacagccgaa gtacgccaaa gaacttaaaa
      661 acacgtgcaa agccgtgctg gcgtgtgtgg gagtctggat aatgaccctg accacgacca
      721 cccctctgct actgctctat aaagacccag ataaagactc cactcccgcc acctgcctca
      781 agatttctga catcatctat ctaaaagctg tgaacgtgct gaacctcact cgactgacat
      841 tttttttctt gattcctttg ttcatcatga ttgggtgcta cttggtcatt attcataatc
      901 tccttcacgg caggacgtct aagctgaaac ccaaagtcaa ggagaagtcc ataaggatca
      961 tcatcacgct gctggtgcag gtgctcgtct gctttatgcc cttccacatc tgtttcgctt
     1021 tcctgatgct gggaacgggg gagaacagtt acaatccctg gggagccttt accaccttcc
     1081 tcatgaacct cagcacgtgt ctggatgtga ttctctacta catcgtttca aaacaatttc
     1141 aggctcgagt cattagtgtc atgctatacc gtaattacct tcgaagcatg cgcagaaaaa
     1201 gtttccgatc tggtagtcta cggtcactaa gcaatataaa cagtgaaatg ttatgaataa
     1261 taaggttctt tcatttcaat cccatcaaaa ttcacttcac taactactct ggcgtcaatg
     1321 gatattctgt ataatactat caagtccctt ttctcttgaa aaaataaatt cattatcttc
     1381 attttaaaaa ctta
//
