LOCUS       NM_020752               7023 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens G protein-coupled receptor 158 (GPR158), mRNA.
ACCESSION   NM_020752
VERSION     NM_020752.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7023)
  AUTHORS   Laboute,T., Zucca,S., Holcomb,M., Patil,D.N., Garza,C.,
            Wheatley,B.A., Roy,R.N., Forli,S. and Martemyanov,K.A.
  TITLE     Orphan receptor GPR158 serves as a metabotropic glycine receptor:
            mGlyR
  JOURNAL   Science 379 (6639), 1352-1358 (2023)
   PUBMED   36996198
  REMARK    GeneRIF: Orphan receptor GPR158 serves as a metabotropic glycine
            receptor: mGlyR.
REFERENCE   2  (bases 1 to 7023)
  AUTHORS   Patil,D.N., Singh,S., Laboute,T., Strutzenberg,T.S., Qiu,X., Wu,D.,
            Novick,S.J., Robinson,C.V., Griffin,P.R., Hunt,J.F., Izard,T.,
            Singh,A.K. and Martemyanov,K.A.
  TITLE     Cryo-EM structure of human GPR158 receptor coupled to the
            RGS7-Gbeta5 signaling complex
  JOURNAL   Science 375 (6576), 86-91 (2022)
   PUBMED   34793198
  REMARK    GeneRIF: Cryo-EM structure of human GPR158 receptor coupled to the
            RGS7-Gbeta5 signaling complex.
REFERENCE   3  (bases 1 to 7023)
  AUTHORS   Jeong,E., Kim,Y., Jeong,J. and Cho,Y.
  TITLE     Structure of the class C orphan GPCR GPR158 in complex with
            RGS7-Gbeta5
  JOURNAL   Nat Commun 12 (1), 6805 (2021)
   PUBMED   34815401
  REMARK    GeneRIF: Structure of the class C orphan GPCR GPR158 in complex
            with RGS7-Gbeta5.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 7023)
  AUTHORS   Engqvist,H., Parris,T.Z., Kovacs,A., Nemes,S., Werner Ronnerman,E.,
            De Lara,S., Biermann,J., Sundfeldt,K., Karlsson,P. and Helou,K.
  TITLE     Immunohistochemical validation of COL3A1, GPR158 and PITHD1 as
            prognostic biomarkers in early-stage ovarian carcinomas
  JOURNAL   BMC Cancer 19 (1), 928 (2019)
   PUBMED   31533654
  REMARK    GeneRIF: Elevated GPR158 expression patterns in mucinous ovarian
            carcinoma patients with unfavorable overall survival.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 7023)
  AUTHORS   Hajj,M., De Vita,T., Vol,C., Renassia,C., Bologna,J.C., Brabet,I.,
            Cazade,M., Pastore,M., Blahos,J., Labesse,G., Pin,J.P. and
            Prezeau,L.
  TITLE     Nonclassical Ligand-Independent Regulation of Go Protein by an
            Orphan Class C G-Protein-Coupled Receptor
  JOURNAL   Mol Pharmacol 96 (2), 233-246 (2019)
   PUBMED   31189666
  REMARK    GeneRIF: Data observed that GPR158 interacted with and stabilized
            the amount of RGS7-beta5 through a 50-residue region downstream of
            its transmembrane domain and upstream of the VCPWE motifs. Also,
            two VCPWE motifs are involved in alphao binding.
REFERENCE   6  (bases 1 to 7023)
  AUTHORS   Ovsyannikova,I.G., Kennedy,R.B., O'Byrne,M., Jacobson,R.M.,
            Pankratz,V.S. and Poland,G.A.
  TITLE     Genome-wide association study of antibody response to smallpox
            vaccine
  JOURNAL   Vaccine 30 (28), 4182-4189 (2012)
   PUBMED   22542470
REFERENCE   7  (bases 1 to 7023)
  AUTHORS   Ng,D., Hu,N., Hu,Y., Wang,C., Giffen,C., Tang,Z.Z., Han,X.Y.,
            Yang,H.H., Lee,M.P., Goldstein,A.M. and Taylor,P.R.
  TITLE     Replication of a genome-wide case-control study of esophageal
            squamous cell carcinoma
  JOURNAL   Int J Cancer 123 (7), 1610-1615 (2008)
   PUBMED   18649358
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   8  (bases 1 to 7023)
  AUTHORS   Grupe,A., Li,Y., Rowland,C., Nowotny,P., Hinrichs,A.L., Smemo,S.,
            Kauwe,J.S., Maxwell,T.J., Cherny,S., Doil,L., Tacey,K., van
            Luchene,R., Myers,A., Wavrant-De Vrieze,F., Kaleem,M.,
            Hollingworth,P., Jehu,L., Foy,C., Archer,N., Hamilton,G.,
            Holmans,P., Morris,C.M., Catanese,J., Sninsky,J., White,T.J.,
            Powell,J., Hardy,J., O'Donovan,M., Lovestone,S., Jones,L.,
            Morris,J.C., Thal,L., Owen,M., Williams,J. and Goate,A.
  TITLE     A scan of chromosome 10 identifies a novel locus showing strong
            association with late-onset Alzheimer disease
  JOURNAL   Am J Hum Genet 78 (1), 78-88 (2006)
   PUBMED   16385451
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   9  (bases 1 to 7023)
  AUTHORS   Bjarnadottir,T.K., Fredriksson,R. and Schioth,H.B.
  TITLE     The gene repertoire and the common evolutionary history of
            glutamate, pheromone (V2R), taste(1) and other related G
            protein-coupled receptors
  JOURNAL   Gene 362, 70-84 (2005)
   PUBMED   16229975
REFERENCE   10 (bases 1 to 7023)
  AUTHORS   Deloukas,P., Earthrowl,M.E., Grafham,D.V., Rubenfield,M.,
            French,L., Steward,C.A., Sims,S.K., Jones,M.C., Searle,S.,
            Scott,C., Howe,K., Hunt,S.E., Andrews,T.D., Gilbert,J.G.,
            Swarbreck,D., Ashurst,J.L., Taylor,A., Battles,J., Bird,C.P.,
            Ainscough,R., Almeida,J.P., Ashwell,R.I., Ambrose,K.D.,
            Babbage,A.K., Bagguley,C.L., Bailey,J., Banerjee,R., Bates,K.,
            Beasley,H., Bray-Allen,S., Brown,A.J., Brown,J.Y., Burford,D.C.,
            Burrill,W., Burton,J., Cahill,P., Camire,D., Carter,N.P.,
            Chapman,J.C., Clark,S.Y., Clarke,G., Clee,C.M., Clegg,S., Corby,N.,
            Coulson,A., Dhami,P., Dutta,I., Dunn,M., Faulkner,L., Frankish,A.,
            Frankland,J.A., Garner,P., Garnett,J., Gribble,S., Griffiths,C.,
            Grocock,R., Gustafson,E., Hammond,S., Harley,J.L., Hart,E.,
            Heath,P.D., Ho,T.P., Hopkins,B., Horne,J., Howden,P.J., Huckle,E.,
            Hynds,C., Johnson,C., Johnson,D., Kana,A., Kay,M., Kimberley,A.M.,
            Kershaw,J.K., Kokkinaki,M., Laird,G.K., Lawlor,S., Lee,H.M.,
            Leongamornlert,D.A., Laird,G., Lloyd,C., Lloyd,D.M., Loveland,J.,
            Lovell,J., McLaren,S., McLay,K.E., McMurray,A.,
            Mashreghi-Mohammadi,M., Matthews,L., Milne,S., Nickerson,T.,
            Nguyen,M., Overton-Larty,E., Palmer,S.A., Pearce,A.V., Peck,A.I.,
            Pelan,S., Phillimore,B., Porter,K., Rice,C.M., Rogosin,A.,
            Ross,M.T., Sarafidou,T., Sehra,H.K., Shownkeen,R., Skuce,C.D.,
            Smith,M., Standring,L., Sycamore,N., Tester,J., Thorpe,A.,
            Torcasso,W., Tracey,A., Tromans,A., Tsolas,J., Wall,M., Walsh,J.,
            Wang,H., Weinstock,K., West,A.P., Willey,D.L., Whitehead,S.L.,
            Wilming,L., Wray,P.W., Young,L., Chen,Y., Lovering,R.C.,
            Moschonas,N.K., Siebert,R., Fechtel,K., Bentley,D., Durbin,R.,
            Hubbard,T., Doucette-Stamm,L., Beck,S., Smith,D.R. and Rogers,J.
  TITLE     The DNA sequence and comparative analysis of human chromosome 10
  JOURNAL   Nature 429 (6990), 375-381 (2004)
   PUBMED   15164054
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL139821.9, AY528411.1, AL161654.14 and AL355587.14.
            
            On Nov 23, 2018 this sequence version replaced NM_020752.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803612.226578.1,
                                           SRR1803613.32810.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2145743, SAMEA2148874
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000376351.4/ ENSP00000365529.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-360               AL139821.9         96839-97198
            361-1692            AY528411.1         1-1332
            1693-1696           AL161654.14        56921-56924
            1697-4042           AY528411.1         1337-3682
            4043-7023           AL355587.14        86528-89508
FEATURES             Location/Qualifiers
     source          1..7023
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="10"
                     /map="10p12.1"
     gene            1..7023
                     /gene="GPR158"
                     /gene_synonym="mGlyR"
                     /note="G protein-coupled receptor 158"
                     /db_xref="GeneID:57512"
                     /db_xref="HGNC:HGNC:23689"
                     /db_xref="MIM:614573"
     exon            1..1322
                     /gene="GPR158"
                     /gene_synonym="mGlyR"
                     /inference="alignment:Splign:2.1.0"
     CDS             421..4068
                     /gene="GPR158"
                     /gene_synonym="mGlyR"
                     /note="probable G-protein coupled receptor 158;
                     metabotropic glycine receptor"
                     /codon_start=1
                     /product="metabotropic glycine receptor precursor"
                     /protein_id="NP_065803.2"
                     /db_xref="CCDS:CCDS31166.1"
                     /db_xref="GeneID:57512"
                     /db_xref="HGNC:HGNC:23689"
                     /db_xref="MIM:614573"
                     /translation="MGAMAYPLLLCLLLAQLGLGAVGASRDPQGRPDSPRERTPKGKP
                     HAQQPGRASASDSSAPWSRSTDGTILAQKLAEEVPMDVASYLYTGDSHQLKRANCSGR
                     YELAGLPGKWPALASAHPSLHRALDTLTHATNFLNVMLQSNKSREQNLQDDLDWYQAL
                     VWSLLEGEPSISRAAITFSTDSLSAPAPQVFLQATREESRILLQDLSSSAPHLANATL
                     ETEWFHGLRRKWRPHLHRRGPNQGPRGLGHSWRRKDGLGGDKSHFKWSPPYLECENGS
                     YKPGWLVTLSSAIYGLQPNLVPEFRGVMKVDINLQKVDIDQCSSDGWFSGTHKCHLNN
                     SECMPIKGLGFVLGAYECICKAGFYHPGVLPVNNFRRRGPDQHISGSTKDVSEEAYVC
                     LPCREGCPFCADDSPCFVQEDKYLRLAIISFQALCMLLDFVSMLVVYHFRKAKSIRAS
                     GLILLETILFGSLLLYFPVVILYFEPSTFRCILLRWARLLGFATVYGTVTLKLHRVLK
                     VFLSRTAQRIPYMTGGRVMRMLAVILLVVFWFLIGWTSSVCQNLEKQISLIGQGKTSD
                     HLIFNMCLIDRWDYMTAVAEFLFLLWGVYLCYAVRTVPSAFHEPRYMAVAVHNELIIS
                     AIFHTIRFVLASRLQSDWMLMLYFAHTHLTVTVTIGLLLIPKFSHSSNNPRDDIATEA
                     YEDELDMGRSGSYLNSSINSAWSEHSLDPEDIRDELKKLYAQLEIYKRKKMITNNPHL
                     QKKRCSKKGLGRSIMRRITEIPETVSRQCSKEDKEGADHGTAKGTALIRKNPPESSGN
                     TGKSKEETLKNRVFSLKKSHSTYDHVRDQTEESSSLPTESQEEETTENSTLESLSGKK
                     LTQKLKEDSEAESTESVPLVCKSASAHNLSSEKKTGHPRTSMLQKSLSVIASAKEKTL
                     GLAGKTQTAGVEERTKSQKPLPKDKETNRNHSNSDNTETKDPAPQNSNPAEEPRKPQK
                     SGIMKQQRVNPTTANSDLNPGTTQMKDNFDIGEVCPWEVYDLTPGPVPSESKVQKHVS
                     IVASEMEKNPTFSLKEKSHHKPKAAEVCQQSNQKRIDKAEVCLWESQGQSILEDEKLL
                     ISKTPVLPERAKEENGGQPRAANVCAGQSEELPPKAVASKTENENLNQIGHQEKKTSS
                     SEENVRGSYNSSNNFQQPLTSRAEVCPWEFETPAQPNAGRSVALPASSALSANKIAGP
                     RKEEIWDSFKV"
     exon            1323..1428
                     /gene="GPR158"
                     /gene_synonym="mGlyR"
                     /inference="alignment:Splign:2.1.0"
     exon            1429..1531
                     /gene="GPR158"
                     /gene_synonym="mGlyR"
                     /inference="alignment:Splign:2.1.0"
     exon            1532..1755
                     /gene="GPR158"
                     /gene_synonym="mGlyR"
                     /inference="alignment:Splign:2.1.0"
     exon            1756..1824
                     /gene="GPR158"
                     /gene_synonym="mGlyR"
                     /inference="alignment:Splign:2.1.0"
     exon            1825..1934
                     /gene="GPR158"
                     /gene_synonym="mGlyR"
                     /inference="alignment:Splign:2.1.0"
     exon            1935..2173
                     /gene="GPR158"
                     /gene_synonym="mGlyR"
                     /inference="alignment:Splign:2.1.0"
     exon            2174..2312
                     /gene="GPR158"
                     /gene_synonym="mGlyR"
                     /inference="alignment:Splign:2.1.0"
     exon            2313..2418
                     /gene="GPR158"
                     /gene_synonym="mGlyR"
                     /inference="alignment:Splign:2.1.0"
     exon            2419..2565
                     /gene="GPR158"
                     /gene_synonym="mGlyR"
                     /inference="alignment:Splign:2.1.0"
     exon            2566..7023
                     /gene="GPR158"
                     /gene_synonym="mGlyR"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7002..7007
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GPR158"
                     /gene_synonym="mGlyR"
                     /note="hexamer: AATAAA"
     polyA_site      7023
                     /gene="GPR158"
                     /gene_synonym="mGlyR"
                     /note="major polyA site"
ORIGIN      
        1 atggagcagc gtcttcggcg gccgcggcgg cagcagcagc agcagcttct gaacgcgcct
       61 caatgagagc ggcggtggcg gcagccgggc cgagagacgg actcgggctg actccagccg
      121 ctgggagcgc gaggccatgt aacccgctcg gctccaggct gcgaggtgcg taatccccag
      181 ccggcccctc gcgcagcggg cacggccagc gctgccacag gtgacttgat ttctgcgacg
      241 gtagcttagc cacccggggc caatctcgaa acattcttat tttcaagtcc tttggactgg
      301 gtgccatctg gagaaggggg aagactcctc gaaaaagtct gactgttgag aaactgacga
      361 tccaaattta aaaagtgatt cccccccctc ccgttccctc ctcttctctc tgggaggcag
      421 atgggagcca tggcttaccc cttactcctc tgcctcctgc ttgctcagct gggattggga
      481 gctgttggcg ccagccgcga cccccaagga cggccggatt cccctcgaga gaggaccccg
      541 aaggggaagc cgcacgccca gcagccgggt cgagcctctg cctcggactc ctcggctccc
      601 tggagccgct ccaccgatgg caccatcttg gcgcagaaac tcgccgagga ggtgcccatg
      661 gacgtggcct cttacctcta caccggggac tcccaccagc tgaagcgagc caactgctcc
      721 ggccgctacg agttggcggg cctgccgggg aagtggccag ccctggccag cgcgcacccc
      781 tccttgcacc gggcgctgga cacactgaca cacgccacca acttcctcaa cgtgatgctg
      841 cagagcaata agtcgcggga gcagaacttg caggacgacc tggattggta ccaggcgctg
      901 gtgtggagcc ttctggaggg cgagcccagc atctcccggg cggccatcac cttcagcacc
      961 gattcgctgt ccgcaccggc cccacaggtc ttcctccagg ccacgcgcga ggagagccgc
     1021 atcctgctcc aagacctgtc ctcctccgca ccccacctgg ccaacgccac tctggagacc
     1081 gagtggttcc acggcctccg gcgcaagtgg aggccccact tacaccgccg cggccccaat
     1141 caggggcccc ggggcctggg ccacagctgg cggcgcaagg acgggctcgg cggggacaag
     1201 agccacttca agtggtctcc gccttatctg gagtgcgaga acgggagtta caagcccggg
     1261 tggctggtta ctctttcctc tgccatctac gggttgcagc ctaacctggt cccggaattc
     1321 aggggtgtca tgaaagttga cataaatctt cagaaagtgg acattgacca atgctcaagt
     1381 gatggctggt tttcaggaac tcataaatgc cacctcaaca attcagagtg tatgccaatt
     1441 aaaggcctag gattcgttct tggagcctat gagtgcattt gcaaagcagg attctatcat
     1501 cctggagtct taccagtgaa caactttcgg agaaggggtc cggatcagca tatttcagga
     1561 agtacaaaag atgtgtcaga agaagcctat gtctgcctac cttgcaggga gggctgcccc
     1621 ttctgtgctg atgacagccc atgcttcgtc caggaagata agtatttacg acttgccatc
     1681 atctccttcc aagccctgtg tatgctgctc gacttcgtta gcatgctggt ggtctaccac
     1741 tttcgcaaag caaagagcat ccgggcatcg ggccttatcc tgttggaaac gatccttttt
     1801 ggatctctgc tcctatactt tccagttgtt attttgtact ttgagccaag cacatttcgc
     1861 tgtattctcc taagatgggc tcgtcttctc ggttttgcta ctgtttacgg aactgtcact
     1921 ctcaaacttc acagggtttt gaaggtgttt ctttcacgaa cggctcaacg aattccatat
     1981 atgactggcg gacgggtcat gaggatgctg gcagtaatac tcttggtagt gttttggttt
     2041 ctcattggct ggacttcatc tgtgtgccag aatttggaga aacagatttc acttattggc
     2101 caggggaaaa catccgatca cctcatcttc aatatgtgcc tcattgaccg ctgggactac
     2161 atgacagcag ttgctgaatt tttattcctc ttgtggggtg tttatctctg ctatgcagtg
     2221 cggacagtcc catcggcatt ccatgagccc cgctatatgg ctgttgcagt tcacaatgag
     2281 ctcatcatct ctgctatatt ccatacaatt agatttgttc ttgcctcaag acttcagtct
     2341 gattggatgt tgatgctgta ttttgcacat actcatttga ctgtgacagt caccattggg
     2401 ttgcttttga ttccaaagtt ttcacattca agcaataacc cacgagatga tattgctaca
     2461 gaagcatatg aggatgagct agacatgggc cgatctggat cctacctgaa cagcagtatc
     2521 aattcagcct ggagtgagca cagcttggat ccagaggaca ttcgggacga gctgaaaaaa
     2581 ctctatgccc aactggaaat atataaaaga aagaagatga tcacaaacaa cccccacctc
     2641 cagaaaaagc ggtgctcgaa gaagggccta ggtcgttcca tcatgagacg cattacggag
     2701 atcccagaga cagtcagccg gcagtgctct aaagaggaca aggagggcgc cgaccatggc
     2761 acagccaaag gcactgccct catcaggaag aaccccccag agtcttcagg gaacacaggg
     2821 aaatccaagg aggagaccct gaaaaaccga gtcttctcac tcaagaaatc ccacagcact
     2881 tatgaccacg tgagagacca aacggaagag tccagtagcc tacccacaga aagccaagag
     2941 gaggagacaa cagaaaattc cacactggaa tccctgtcgg gtaaaaaact aacacaaaaa
     3001 ctaaaagaag acagcgaggc tgagtccacg gagtcggtgc cgttggtgtg caagtcagca
     3061 agcgctcaca acctcagctc agagaagaaa actgggcacc cacgaacatc gatgttacag
     3121 aagtctctca gtgtcatagc aagcgccaag gagaagactc ttggattagc tgggaaaacc
     3181 caaacagcag gtgtggaaga acgcactaaa tcccagaaac ctttgccaaa agataaagag
     3241 acaaacagaa atcactcaaa ttctgataac acagagacta aagatcctgc cccccaaaac
     3301 tcaaatcctg cggaggagcc aagaaagcct cagaaatctg ggattatgaa acaacaaagg
     3361 gtcaacccca ccactgccaa ttctgacctg aacccaggca ccacccagat gaaggacaac
     3421 tttgacattg gggaggtgtg tccttgggag gtttatgacc tgacccctgg tcctgtgcct
     3481 tcagaatcaa aagttcaaaa gcacgtatct attgtggctt ctgaaatgga gaaaaacccc
     3541 actttttcct taaaggagaa atctcaccac aagcctaagg cagctgaggt ttgtcagcaa
     3601 tccaatcaga agcgcataga taaggctgaa gtatgccttt gggagagcca aggccagtcc
     3661 attttggaag atgagaagct tttgatttcc aagactccag ttctcccaga gagggcaaaa
     3721 gaggagaacg gaggtcagcc tcgtgcagcc aatgtgtgtg ctgggcagag cgaagaactg
     3781 ccccccaaag ctgtagcatc aaaaacagag aatgaaaatc tcaaccaaat aggacaccag
     3841 gaaaaaaaga catcttcttc tgaggagaat gtgcgtggct cctataactc aagtaataac
     3901 ttccagcaac ctttaacatc acgagcagag gtttgtcctt gggagtttga gaccccagct
     3961 caaccaaatg ctggaagaag tgtagcttta cctgcctctt ctgctctaag tgcaaataag
     4021 atagcagggc ctaggaaaga agagatctgg gatagtttta aagtgtagca tctccaggaa
     4081 gaagaggaaa aggagggaac cccggattgg atatgagaca gaagatataa gaatcaaata
     4141 ttcccaagga ggatttgtca atcaaggaaa acatgacaga tggtgaggta aagtcaaagg
     4201 catgggtaga agaggaccag gggggcaaga gcaacaacgt cataatggag aagtcagact
     4261 ttggtcaaga aagtccttcc cttggtaaca ctaggaaaat ctttccattt cagcatgttt
     4321 aaggaaaata gcccacaatg tctgccctga tcaatatgta tccatgggac tttgaagatc
     4381 ctaagccagg taaaccagga gacacagaag acgtaccaga tttgcaaaga aagaaaaggt
     4441 ataagacata tataactgaa attctaagta gctgaccgag aagaacttac tttacctatt
     4501 taaccttgat agcactgcta acttaatgca tcccaaaaat atcttttata ttaatgattg
     4561 ctctcatttt cttataaatg tatgtttcag tatatcgttg tgtctcatat tcaagcattc
     4621 cagattgtat aatttttgca aataactttg gtattatgtg acacaacaca tttatgcaat
     4681 ctgcagctat tcaattgtta ttgcacctta cagaatacct gctatctatc aactttagtt
     4741 gattcttgaa gtacagtaag ctttctctgg cttgggaagc cataactgtt actataaaaa
     4801 cttttagttt tggctgtggt ttatatattg tgactttgaa tttgactcta ttatttcaca
     4861 tcatggtttg ttatactgtc ttaatcaggg ttttttatac aagttgagtt acttgttttg
     4921 cacttcttgt taggactcag aagctttatt aatattggag atcaagtggt cctacttagt
     4981 catatgtctc aataagttaa ggacaactta tccgttgttt attcaaagtc agagatagat
     5041 aacgccttca ttccaattaa ttgtcccttt taactctttc agtatttcct acttagcagc
     5101 atttccaaag gaagaagcta agagtgagaa aaatataccg tgcattatta ttactattgg
     5161 aaagggaaga ctctagggat gacataagaa ttatagcagt actataaacc caggaagttt
     5221 gcctttcaaa aaaaaacaca ggtagctcct gatagcactt tcaagggatt atttttttaa
     5281 agagaaaaat tatggtagca tcaagatcat tgtatggata tatttttatt atgtgtactg
     5341 aaaatacagt attttaaaat accttaaagt atttattctc ataaactctt attcattgct
     5401 tcagctacag gtagaacttg ctgggctcaa atcccaaaga ggttttataa ccttatttat
     5461 tcaaaaccta taaggtggta tggaatcttc attctcccaa gcactggaaa atgtctaagt
     5521 cctgcaaatt gccattgtga gccacttgct cgacatgtaa catgtaaggt ccatttgcaa
     5581 agcaaagcag cccccaaagc atattttata aagcttattg cattccacac tgatctcttg
     5641 gcatgggaat cctaagctgc cgactaagcc ctaccgacat gatcatgcag tgagattcca
     5701 aagctcagtc tcatttcatt taaagaagaa tcactcagaa atagaaccga gacttccctt
     5761 tttctccctg taaacaccca agtatcaact gcttatttgg ccaggacact cccagcacaa
     5821 ataactattt tttatgtcac aagcagcaag gaggacatgc tagggtgata aaagatggag
     5881 aaacaggatc agagggtgga tatagggctg ttcttagaga gtattttcag tggaaggtaa
     5941 aaacagaatt ctccatattc atcatcaaat ttttctcagt gattttttta ttcaggagta
     6001 agcaagcact tcactgtttc acaaagctgt gcgcaaatct tcctcaccca tttgctgact
     6061 ttatgcatta ctcaggttgg tggggtcggt ttgagaagat atagaaattc tatttttgtg
     6121 tctttacacc atttatttct tttatctctt ccttttcaat gaaggcctat atgcttggtg
     6181 acctccttta aggaatcttt gtgaactggg ttggaagttc ctagacccac atatttgttt
     6241 catttatgtc tgaaatctgt tagcacttga ttcctttctt gagaattatg cagtcaagca
     6301 tcagtgactt tctattgcac ttcaggattg atcctgctag agatgtgagt taaaaagact
     6361 tgccaaatta tatcttagcg acattctata gttcatagat tattctccac cagcataaat
     6421 cagtgagagt gcctagagtc tttctgagag tttcattgcc attatcaaca agagaagttg
     6481 aaatttacaa gtcaggaggt tatttttcca gattgataac catagaaagt gaataaacac
     6541 ttttaaggtc gcaaacattt gctaggttgt ccttctcaat gcatgtgcag gctgcatcct
     6601 gtccttgttt ttaagccagg gtttataaat aagtagattt ataccaatct taatagaatt
     6661 gtatatttta tgcaagaatt aaatgcttta caacatgaag tataactcaa cccattgtaa
     6721 actttggtgg caatatggat ttgaaactcg acagttctct tgtatttgct tcctaggttt
     6781 ctgcatgcaa gttatgacag gtaggactga aaaaacactg ccttttgact tctagcattt
     6841 agcaaccgag agtcgtagag tcaataaagc tgtaagtgtc ttcacttaat ctgtggttct
     6901 cctaaaacta ttatctgaaa cctacagcat cccaccatga aatatttggt aaatttatgt
     6961 tgtgacgtgt tgcagcatgt aaataattat aacttctctg caataaaaca tatttatatg
     7021 aaa
//
