LOCUS       NM_005288               4851 bp    mRNA    linear   PRI 01-JUN-2024
DEFINITION  Homo sapiens G protein-coupled receptor 12 (GPR12), mRNA.
ACCESSION   NM_005288
VERSION     NM_005288.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4851)
  AUTHORS   Pan,G., Jiang,B., Yi,Z., Yin,J. and Liu,Y.
  TITLE     Exosomal miR-105-5p derived from bladder cancer stem cells targets
            for GPR12 to promote the malignancy of bladder cancer
  JOURNAL   BMC Urol 23 (1), 155 (2023)
   PUBMED   37789353
  REMARK    GeneRIF: Exosomal miR-105-5p derived from bladder cancer stem cells
            targets for GPR12 to promote the malignancy of bladder cancer.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4851)
  AUTHORS   Li,H., Zhang,J., Yu,Y., Luo,F., Wu,L., Liu,J., Chen,N., Liu,Z. and
            Hua,T.
  TITLE     Structural insight into the constitutive activity of human orphan
            receptor GPR12
  JOURNAL   Sci Bull (Beijing) 68 (1), 95-104 (2023)
   PUBMED   36593162
  REMARK    GeneRIF: Structural insight into the constitutive activity of human
            orphan receptor GPR12.
REFERENCE   3  (bases 1 to 4851)
  AUTHORS   Zhang,M., Yang,X., Chen,S., Jia,W., Ma,X., Wang,J., Qian,Y.,
            Lei,D., Liu,H. and Pan,X.
  TITLE     GPR12 inhibits migration and promotes apoptosis in esophageal
            cancer and hypopharyngeal cancer cells
  JOURNAL   Thorac Cancer 12 (10), 1525-1535 (2021)
   PUBMED   33742771
  REMARK    GeneRIF: GPR12 inhibits migration and promotes apoptosis in
            esophageal cancer and hypopharyngeal cancer cells.
REFERENCE   4  (bases 1 to 4851)
  AUTHORS   Brown,K.J., Laun,A.S. and Song,Z.H.
  TITLE     Cannabidiol, a novel inverse agonist for GPR12
  JOURNAL   Biochem Biophys Res Commun 493 (1), 451-454 (2017)
   PUBMED   28888984
  REMARK    GeneRIF: Since we have demonstrated that CBD is an inverse agonist
            for GPR12, this provides novel mechanism of action for CBD, and an
            initial chemical scaffold upon which highly potent and efficacious
            agents acting on GPR12 may be developed with the ultimate goal of
            blocking cancer metastasis.
REFERENCE   5  (bases 1 to 4851)
  AUTHORS   Lu,X., Zhang,N., Meng,B., Dong,S. and Hu,Y.
  TITLE     Involvement of GPR12 in the regulation of cell proliferation and
            survival
  JOURNAL   Mol Cell Biochem 366 (1-2), 101-110 (2012)
   PUBMED   22430950
  REMARK    GeneRIF: GPR12 may play a role in cell proliferation and survival
REFERENCE   6  (bases 1 to 4851)
  AUTHORS   Uhlenbrock,K., Huber,J., Ardati,A., Busch,A.E. and Kostenis,E.
  TITLE     Fluid shear stress differentially regulates gpr3, gpr6, and gpr12
            expression in human umbilical vein endothelial cells
  JOURNAL   Cell Physiol Biochem 13 (2), 75-84 (2003)
   PUBMED   12649592
  REMARK    GeneRIF: control of gene expression in vascular endothelial cells
            in the presence of fluid shear stress and classifies it as a
            sphingosine 1-phosphate receptor
REFERENCE   7  (bases 1 to 4851)
  AUTHORS   Song,Z.H., Modi,W. and Bonner,T.I.
  TITLE     Molecular cloning and chromosomal localization of human genes
            encoding three closely related G protein-coupled receptors
  JOURNAL   Genomics 28 (2), 347-349 (1995)
   PUBMED   8530049
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL159978.14, AK290220.1 and AW015633.1.
            
            On Nov 23, 2018 this sequence version replaced NM_005288.3.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660803.250970.1,
                                           SRR1803615.90676.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2142853, SAMEA2145743
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000405846.5/ ENSP00000384932.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1                 AL159978.14        83552-83552         c
            2-1290              AK290220.1         2-1290
            1291-4508           AL159978.14        78309-81526         c
            4509-4851           AW015633.1         14-356              c
FEATURES             Location/Qualifiers
     source          1..4851
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="13"
                     /map="13q12.13"
     gene            1..4851
                     /gene="GPR12"
                     /gene_synonym="GPCR12; GPCR21; PPP1R84"
                     /note="G protein-coupled receptor 12"
                     /db_xref="GeneID:2835"
                     /db_xref="HGNC:HGNC:4466"
                     /db_xref="MIM:600752"
     exon            1..208
                     /gene="GPR12"
                     /gene_synonym="GPCR12; GPCR21; PPP1R84"
                     /inference="alignment:Splign:2.1.0"
     exon            209..4851
                     /gene="GPR12"
                     /gene_synonym="GPCR12; GPCR21; PPP1R84"
                     /inference="alignment:Splign:2.1.0"
     CDS             224..1228
                     /gene="GPR12"
                     /gene_synonym="GPCR12; GPCR21; PPP1R84"
                     /note="protein phosphatase 1, regulatory subunit 84"
                     /codon_start=1
                     /product="G-protein coupled receptor 12"
                     /protein_id="NP_005279.1"
                     /db_xref="CCDS:CCDS9319.1"
                     /db_xref="GeneID:2835"
                     /db_xref="HGNC:HGNC:4466"
                     /db_xref="MIM:600752"
                     /translation="MNEDLKVNLSGLPRDYLDAAAAENISAAVSSRVPAVEPEPELVV
                     NPWDIVLCTSGTLISCENAIVVLIIFHNPSLRAPMFLLIGSLALADLLAGIGLITNFV
                     FAYLLQSEATKLVTIGLIVASFSASVCSLLAITVDRYLSLYYALTYHSERTVTFTYVM
                     LVMLWGTSICLGLLPVMGWNCLRDESTCSVVRPLTKNNAAILSVSFLFMFALMLQLYI
                     QICKIVMRHAHQIALQHHFLATSHYVTTRKGVSTLAIILGTFAACWMPFTLYSLIADY
                     TYPSIYTYATLLPATYNSIINPVIYAFRNQEIQKALCLICCGCIPSSLAQRARSPSDV
                     "
     regulatory      1597..1602
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GPR12"
                     /gene_synonym="GPCR12; GPCR21; PPP1R84"
                     /note="hexamer: AATAAA"
     polyA_site      1625
                     /gene="GPR12"
                     /gene_synonym="GPCR12; GPCR21; PPP1R84"
     regulatory      3009..3014
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GPR12"
                     /gene_synonym="GPCR12; GPCR21; PPP1R84"
                     /note="hexamer: AATAAA"
     polyA_site      3032
                     /gene="GPR12"
                     /gene_synonym="GPCR12; GPCR21; PPP1R84"
     regulatory      4815..4820
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GPR12"
                     /gene_synonym="GPCR12; GPCR21; PPP1R84"
                     /note="hexamer: AATAAA"
     polyA_site      4851
                     /gene="GPR12"
                     /gene_synonym="GPCR12; GPCR21; PPP1R84"
                     /note="major polyA site"
ORIGIN      
        1 gaaagctggg cgcgcgagcc gaggctgctg ctcacctgca gccgcgccgc tcacctgccg
       61 cggagtgcgc gccccgcgcc ctcccggccc gctccggagc ccggccgccc cccccttgcc
      121 acagcgggca actgcgccga catgcgacac gagtagccca cgcgcttcgg aagcaccgga
      181 ggagccccct cggcctgggc gagccaagag gacaggggtt aaaatgaatg aagacctgaa
      241 ggtcaattta agcgggctgc ctcgggatta tttagatgcc gctgctgcgg agaacatctc
      301 ggctgctgtc tcctcccggg ttcctgccgt agagccagag cctgagctcg tagtcaaccc
      361 ctgggacatt gtcttgtgta cctcgggaac cctcatctcc tgtgaaaatg ccattgtggt
      421 ccttatcatc ttccacaacc ccagcctgcg agcacccatg ttcctgctaa taggcagcct
      481 ggctcttgca gacctgctgg ccggcattgg actcatcacc aattttgttt ttgcctacct
      541 gcttcagtca gaagccacca agctggtcac gatcggcctc attgtcgcct ctttctctgc
      601 ctctgtctgc agcttgctgg ctatcactgt tgaccgctac ctctcactgt actacgctct
      661 gacgtaccat tcggagagga cggtcacgtt tacctatgtc atgctcgtca tgctctgggg
      721 gacctccatc tgcctggggc tgctgcccgt catgggctgg aactgcctcc gagacgagtc
      781 cacctgcagc gtggtcagac cgctcaccaa gaacaacgcg gccatcctct cggtgtcctt
      841 cctcttcatg tttgcgctca tgcttcagct ctacatccag atctgtaaga ttgtgatgag
      901 gcacgcccat cagatagccc tgcagcacca cttcctggcc acgtcgcact atgtgaccac
      961 ccggaaaggg gtctccaccc tggctatcat cctggggacg tttgctgctt gctggatgcc
     1021 tttcaccctc tattccttga tagcggatta cacctacccc tccatctata cctacgccac
     1081 cctcctgccc gccacctaca attccatcat caaccctgtc atatatgctt tcagaaacca
     1141 agagatccag aaagcgctct gtctcatttg ctgcggctgc atcccgtcca gtctcgccca
     1201 gagagcgcgc tcgcccagtg atgtgtagca cccttgcacc caggaggact ctgcatttac
     1261 caagcacttc cactgcctgg ccaaggtttg agatgcttcc cttgaattcc ttgcattgga
     1321 ttccctctca agccacagga gcacttagca ttcgtgaaac aatgacatcg ttcagatgag
     1381 ttaagtgatt gaagtgaaaa taatgttacc agtgttttca ccattaaaaa aaattgttga
     1441 gctctccact caacattatt ttgttttgct tgggggcaag ggtttccttt tatactttgt
     1501 ttggagggct gactgggggt aggaaaggaa gggatatgat acgcccatga tgttattaga
     1561 agtaaatgag tttcagagtt tttacctgga ctttaaaata aatttgagtg attgaggaaa
     1621 attgagaaag acttactttt tccagactat tttttttttt tacatgatgg tagatttttt
     1681 aatgctgcat gtatgtaaca gaatatgacc cctttcaatc caatacaaat agtttacata
     1741 attgtgtttg gaagggacct gattttgcta acatgaactc agtaatgtca ccgacactga
     1801 aaccgtgaac ccattcttat ccattctttc tttcctttcg gcaattactg ttcacgagaa
     1861 ttccttcggt atttctataa atgttgcaac tccttctgca tggtcgactg tgttagctag
     1921 accacttgtt tcaaatgttg ccatgtcaat ctagattcca acgggtcatt tttcaatata
     1981 gttttccaat atgtactttc taaaatgaat cctgaagtag gttttgatct tcatatgaag
     2041 taggattgat tagatccacc attgtagttt cttaagctat tgtctgattg ttttcagaaa
     2101 acaaaaggaa aaaatattag caattgagag tagattaaat ttattttaat atattctgaa
     2161 aaaaactaat gtgacattat catgaaatga aaaaaatcac ctttcctctc tctctgagaa
     2221 tatactaatg gttattcttg attgagataa ttagaataaa tattataggc caagtttttc
     2281 tggttcatac tgtctgtggt gcataatgca tttgaaacag gcagtcccct attttaatga
     2341 ttaggtttgt ttgtcagtgc acagcgtcca tctatgactg tgacatatac cagctatatc
     2401 ctgtaaggtc ataaacagaa tgaccattgg ctgttgatga aaagttaagt ttgaaatctt
     2461 ttaactcctt ttcaccaaaa accaatttat acacaataaa aatcacattg cctgttttca
     2521 gagaacaaga atgtagtcag gaaattgcaa agaagaattc actttaaaca cattgtaaaa
     2581 tcgatgtgtc tcaggtttct tttctgttca tgcagacaaa tgtagacgca ggtaccttcc
     2641 ctccatttgc aatggattta tagttatgtc tatataatca gtggaaaaaa ttcttggcag
     2701 cacattaatt gagcccattc agatggcagc aaaagcagta tttcagggtc attggggaat
     2761 catttgtaat aaattaacct catcggtttg tcccagatgt gatgcccagg gtgctctcaa
     2821 gaccattctg ggagcctgca tgctgatgcc ttctctccag agcatcttct aatgtagcac
     2881 acgtgtgaaa aaatcctcta cttgaatctg tgtctcctcc ctgaggttac ttatggaagc
     2941 aggagaggga ctgaaatact taagaagtgg gaaagaattt tcaatacata ccactgtatg
     3001 atcaatctaa taaaactgat gcctttaaaa catttctttt gaacatttga tggagttagt
     3061 aggttgaaca tgatgagtct gttcgttttt aattaattaa aattgaatag caccaccata
     3121 ggagacttga caaaggtaag ttggttttga gaggggctag gaaatatccg taatgaggtt
     3181 ttaaaggaat aaatgatgat tttataaaaa tagtggctcc atttgcatat ctccaatagt
     3241 cagaattata aagagaataa tatgtacttg ttgtgaccac atgtgtgcca ggccccatgc
     3301 tgcgtgtttt actataatca caaaactact acttttatag gtgagaaaac taagactaag
     3361 agaggcgagg ataaaaaatg tgcccgaggc cacaaagcac aaggatagag tcagaatttg
     3421 aagccagctt ttccaaagtc taagtcttgc tccttccctg taccttgcaa gctttcatcc
     3481 ctcagtggat accagggctg aggaaactga tttgtaacac gttcctgtca cagagttttg
     3541 gctctacatt taagacataa aacttggcct ggcgcagtgg ctcacgcctg taatcccagc
     3601 actttggggg gccaaggcag gtggatcacc tgaggtcagg agttcgaaac cagcctgacc
     3661 aatatggcaa aaccctgtct ctactaaaaa taaaatttag ccgggcgtgg tggcgggtgc
     3721 ctgtaatccc agctactcag gaggctgagg caggagaact gcttgaagac aggaggcaga
     3781 ggtagcagtg aacaaagatc gcaccactgc actccagcct gggtgacaga gcaagactcc
     3841 atctcaaaaa aaaaaggaca taaaatttcc tgtttaaagt tacactagtt acgtggttgc
     3901 ttttggccca tgtttttaaa acatatagtt tcagctaaga gatttttcag ttcaactaac
     3961 aacatgcctt taccgtttaa attgtgaaaa atgttggata tttatgtggt tttctacaag
     4021 gtttcttttt tcctgtggag ctaattattt ttaaatgtgc acacatctca atgtacatat
     4081 gtgtatgtat gtatttatgc atacgtatac ccaataatat taactttgca acaggaccaa
     4141 agacctcatg gaaaactgtc ttatttccaa cagtcctgag gtagcagata atcttttact
     4201 ccacataatt aaaatttcta gctaccaagg aattgatatt tttccagtag aaacagttat
     4261 ccttgtaact aggacagttc cattacttta ccagaatcct gaattagtca aaactagtca
     4321 tcgaagttat gactatctag ggttttgttt ccttttattc ctttaactct gtatttcttt
     4381 ctatcagtga agtttgtttt taaaggtacc tggggcaggg aagcagggtg gagatagagc
     4441 ccctccttgc ctggagccta cagtaaggag ccaggttcag gaggagtgga ttccttggtt
     4501 gacttggcca tgcagaaata gtcttagact cccccaggat ttaaaatagg attgaaacaa
     4561 gaagtttaaa agagcagagg aggaatagag atacttagtg ccaataaaaa tatcatggag
     4621 acttcttcgg ctttttaaaa aaaatgctta atccattatt aaaataatga ttgactttat
     4681 tttatagtaa tatgtgataa tatgaagtta ggatgacttg ccatttcaga tttaaaatac
     4741 atattaatta agtgactact atgttgtgat tttgaataat gtttaagttt ctatattgat
     4801 tgtatgcctg gtataataaa ggaaaagact gccttattgt cccctttaaa a
//
