LOCUS       NM_002510               2650 bp    mRNA    linear   PRI 11-SEP-2024
DEFINITION  Homo sapiens glycoprotein nmb (GPNMB), transcript variant 2, mRNA.
ACCESSION   NM_002510
VERSION     NM_002510.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Yu,X., Li,M., Wang,C. and Guan,X.
  TITLE     Glycoprotein non-metastatic melanoma protein B (GPNMB): An
            attractive target in atherosclerosis
  JOURNAL   Biochem Biophys Res Commun 732, 150386 (2024)
   PUBMED   39024681
  REMARK    GeneRIF: Glycoprotein non-metastatic melanoma protein B (GPNMB): An
            attractive target in atherosclerosis.
            Review article
REFERENCE   2  (bases 1 to 2650)
  AUTHORS   Vakayil,M., Madani,A.Y., Agha,M.V., Majeed,Y., Hayat,S.,
            Yonuskunju,S., Mohamoud,Y.A., Malek,J., Suhre,K. and Mazloum,N.A.
  TITLE     The E3 ubiquitin-protein ligase UHRF1 promotes adipogenesis and
            limits fibrosis by suppressing GPNMB-mediated TGF-beta signaling
  JOURNAL   Sci Rep 14 (1), 11886 (2024)
   PUBMED   38789534
  REMARK    GeneRIF: The E3 ubiquitin-protein ligase UHRF1 promotes
            adipogenesis and limits fibrosis by suppressing GPNMB-mediated
            TGF-beta signaling.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2650)
  AUTHORS   Gibbons,E., Taya,M., Wu,H., Lopa,S.H., Moss,J., Henske,E.P.,
            McCormack,F.X. and Hammes,S.R.
  TITLE     Glycoprotein non-metastatic melanoma protein B promotes tumor
            growth and is a biomarker for lymphangioleiomyomatosis
  JOURNAL   Endocr Relat Cancer 31 (6), e230312 (2024)
   PUBMED   38614127
  REMARK    GeneRIF: Glycoprotein non-metastatic melanoma protein B promotes
            tumor growth and is a biomarker for lymphangioleiomyomatosis.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2650)
  AUTHORS   Kimura,T., Okita,Y., Nagumo,Y., Chin,J.M., Fikry,M.A., Shiga,M.,
            Kandori,S., Kawahara,T., Suzuki,H., Nishiyama,H. and Kato,M.
  TITLE     Glycoprotein nonmetastatic melanoma protein B impacts the malignant
            potential of bladder cancer cells through its hem-immunoreceptor
            tyrosine-based activation motif
  JOURNAL   Pathol Int 74 (5), 262-273 (2024)
   PUBMED   38501371
  REMARK    GeneRIF: Glycoprotein nonmetastatic melanoma protein B impacts the
            malignant potential of bladder cancer cells through its
            hem-immunoreceptor tyrosine-based activation motif.
REFERENCE   5  (bases 1 to 2650)
  AUTHORS   Yalcin,F., Haneke,H., Efe,I.E., Kuhrt,L.D., Motta,E., Nickl,B.,
            Fluh,C., Synowitz,M., Dzaye,O., Bader,M. and Kettenmann,H.
  TITLE     Tumor associated microglia/macrophages utilize GPNMB to promote
            tumor growth and alter immune cell infiltration in glioma
  JOURNAL   Acta Neuropathol Commun 12 (1), 50 (2024)
   PUBMED   38566120
  REMARK    GeneRIF: Tumor associated microglia/macrophages utilize GPNMB to
            promote tumor growth and alter immune cell infiltration in glioma.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2650)
  AUTHORS   Bandari,P.S., Qian,J., Yehia,G., Joshi,D.D., Maloof,P.B.,
            Potian,J., Oh,H.S., Gascon,P., Harrison,J.S. and Rameshwar,P.
  TITLE     Hematopoietic growth factor inducible neurokinin-1 type: a
            transmembrane protein that is similar to neurokinin 1 interacts
            with substance P
  JOURNAL   Regul Pept 111 (1-3), 169-178 (2003)
   PUBMED   12609765
REFERENCE   7  (bases 1 to 2650)
  AUTHORS   Basrur,V., Yang,F., Kushimoto,T., Higashimoto,Y., Yasumoto,K.,
            Valencia,J., Muller,J., Vieira,W.D., Watabe,H., Shabanowitz,J.,
            Hearing,V.J., Hunt,D.F. and Appella,E.
  TITLE     Proteomic analysis of early melanosomes: identification of novel
            melanosomal proteins
  JOURNAL   J Proteome Res 2 (1), 69-79 (2003)
   PUBMED   12643545
REFERENCE   8  (bases 1 to 2650)
  AUTHORS   Bachner,D., Schroder,D. and Gross,G.
  TITLE     mRNA expression of the murine glycoprotein (transmembrane) nmb
            (Gpnmb) gene is linked to the developing retinal pigment epithelium
            and iris
  JOURNAL   Brain Res Gene Expr Patterns 1 (3-4), 159-165 (2002)
   PUBMED   12638126
REFERENCE   9  (bases 1 to 2650)
  AUTHORS   Ahn,J.H., Lee,Y., Jeon,C., Lee,S.J., Lee,B.H., Choi,K.D. and
            Bae,Y.S.
  TITLE     Identification of the genes differentially expressed in human
            dendritic cell subsets by cDNA subtraction and microarray analysis
  JOURNAL   Blood 100 (5), 1742-1754 (2002)
   PUBMED   12176896
REFERENCE   10 (bases 1 to 2650)
  AUTHORS   Weterman,M.A., Ajubi,N., van Dinter,I.M., Degen,W.G., van
            Muijen,G.N., Ruitter,D.J. and Bloemers,H.P.
  TITLE     nmb, a novel gene, is expressed in low-metastatic human melanoma
            cell lines and xenografts
  JOURNAL   Int J Cancer 60 (1), 73-81 (1995)
   PUBMED   7814155
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from CD105306.1, BC011595.1,
            BC032783.1 and BC025297.1.
            
            On Jun 2, 2019 this sequence version replaced NM_002510.2.
            
            Summary: The protein encoded by this gene is a type I transmembrane
            glycoprotein which shows homology to the pMEL17 precursor, a
            melanocyte-specific protein. GPNMB shows expression in the lowly
            metastatic human melanoma cell lines and xenografts but does not
            show expression in the highly metastatic cell lines. GPNMB may be
            involved in growth delay and reduction of metastatic potential. Two
            transcript variants encoding different isoforms have been found for
            this gene. [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (2) uses an alternate in-frame
            splice site compared to variant 1. The resulting isoform (b) has
            the same N- and C-termini but is shorter compared to isoform a.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803617.79056.1,
                                           SRR7346977.1135135.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000258733.9/ ENSP00000258733.5
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-319               CD105306.1         67-385
            320-472             BC011595.1         286-438
            473-1101            BC032783.1         467-1095
            1102-1543           BC032783.1         1132-1573
            1544-2650           BC025297.1         1512-2618
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="7"
                     /map="7p15.3"
     gene            1..2650
                     /gene="GPNMB"
                     /gene_synonym="HGFIN; NMB; PLCA3"
                     /note="glycoprotein nmb"
                     /db_xref="GeneID:10457"
                     /db_xref="HGNC:HGNC:4462"
                     /db_xref="MIM:604368"
     exon            1..153
                     /gene="GPNMB"
                     /gene_synonym="HGFIN; NMB; PLCA3"
                     /inference="alignment:Splign:2.1.0"
     CDS             84..1766
                     /gene="GPNMB"
                     /gene_synonym="HGFIN; NMB; PLCA3"
                     /note="isoform b precursor is encoded by transcript
                     variant 2; osteoactivin; transmembrane glycoprotein NMB;
                     glycoprotein nmb-like protein; transmembrane glycoprotein
                     HGFIN; glycoprotein (transmembrane) nmb; glycoprotein
                     nonmetastatic melanoma protein B; hematopoietic growth
                     factor inducible neurokinin-1 type"
                     /codon_start=1
                     /product="transmembrane glycoprotein NMB isoform b
                     precursor"
                     /protein_id="NP_002501.1"
                     /db_xref="CCDS:CCDS5380.1"
                     /db_xref="GeneID:10457"
                     /db_xref="HGNC:HGNC:4462"
                     /db_xref="MIM:604368"
                     /translation="MECLYYFLGFLLLAARLPLDAAKRFHDVLGNERPSAYMREHNQL
                     NGWSSDENDWNEKLYPVWKRGDMRWKNSWKGGRVQAVLTSDSPALVGSNITFAVNLIF
                     PRCQKEDANGNIVYEKNCRNEAGLSADPYVYNWTAWSEDSDGENGTGQSHHNVFPDGK
                     PFPHHPGWRRWNFIYVFHTLGQYFQKLGRCSVRVSVNTANVTLGPQLMEVTVYRRHGR
                     AYVPIAQVKDVYVVTDQIPVFVTMFQKNDRNSSDETFLKDLPIMFDVLIHDPSHFLNY
                     STINYKWSFGDNTGLFVSTNHTVNHTYVLNGTFSLNLTVKAAAPGPCPPPPPPPRPSK
                     PTPSLGPAGDNPLELSRIPDENCQINRYGHFQATITIVEGILEVNIIQMTDVLMPVPW
                     PESSLIDFVVTCQGSIPTEVCTIISDPTCEITQNTVCSPVDVDEMCLLTVRRTFNGSG
                     TYCVNLTLGDDTSLALTSTLISVPDRDPASPLRMANSALISVGCLAIFVTVISLLVYK
                     KHKEYNPIENSPGNVVRSKGLSVFLNRAKAVFFPGNQEKDPLLKNQEFKGVS"
     exon            154..306
                     /gene="GPNMB"
                     /gene_synonym="HGFIN; NMB; PLCA3"
                     /inference="alignment:Splign:2.1.0"
     exon            307..450
                     /gene="GPNMB"
                     /gene_synonym="HGFIN; NMB; PLCA3"
                     /inference="alignment:Splign:2.1.0"
     exon            451..624
                     /gene="GPNMB"
                     /gene_synonym="HGFIN; NMB; PLCA3"
                     /inference="alignment:Splign:2.1.0"
     exon            625..783
                     /gene="GPNMB"
                     /gene_synonym="HGFIN; NMB; PLCA3"
                     /inference="alignment:Splign:2.1.0"
     exon            784..1101
                     /gene="GPNMB"
                     /gene_synonym="HGFIN; NMB; PLCA3"
                     /inference="alignment:Splign:2.1.0"
     exon            1102..1200
                     /gene="GPNMB"
                     /gene_synonym="HGFIN; NMB; PLCA3"
                     /inference="alignment:Splign:2.1.0"
     exon            1201..1303
                     /gene="GPNMB"
                     /gene_synonym="HGFIN; NMB; PLCA3"
                     /inference="alignment:Splign:2.1.0"
     exon            1304..1512
                     /gene="GPNMB"
                     /gene_synonym="HGFIN; NMB; PLCA3"
                     /inference="alignment:Splign:2.1.0"
     exon            1513..1606
                     /gene="GPNMB"
                     /gene_synonym="HGFIN; NMB; PLCA3"
                     /inference="alignment:Splign:2.1.0"
     exon            1607..2650
                     /gene="GPNMB"
                     /gene_synonym="HGFIN; NMB; PLCA3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2629..2634
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GPNMB"
                     /gene_synonym="HGFIN; NMB; PLCA3"
                     /note="hexamer: AATAAA"
     polyA_site      2650
                     /gene="GPNMB"
                     /gene_synonym="HGFIN; NMB; PLCA3"
                     /note="major polyA site"
ORIGIN      
        1 agaagaacac tgttgctctt ggtggacggg cccagaggaa ttcagagtta aaccttgagt
       61 gcctgcgtcc gtgagaattc agcatggaat gtctctacta tttcctggga tttctgctcc
      121 tggctgcaag attgccactt gatgccgcca aacgatttca tgatgtgctg ggcaatgaaa
      181 gaccttctgc ttacatgagg gagcacaatc aattaaatgg ctggtcttct gatgaaaatg
      241 actggaatga aaaactctac ccagtgtgga agcggggaga catgaggtgg aaaaactcct
      301 ggaagggagg ccgtgtgcag gcggtcctga ccagtgactc accagccctc gtgggctcaa
      361 atataacatt tgcggtgaac ctgatattcc ctagatgcca aaaggaagat gccaatggca
      421 acatagtcta tgagaagaac tgcagaaatg aggctggttt atctgctgat ccgtatgttt
      481 acaactggac agcatggtca gaggacagtg acggggaaaa tggcaccggc caaagccatc
      541 ataacgtctt ccctgatggg aaaccttttc ctcaccaccc cggatggaga agatggaatt
      601 tcatctacgt cttccacaca cttggtcagt atttccagaa attgggacga tgttcagtga
      661 gagtttctgt gaacacagcc aatgtgacac ttgggcctca actcatggaa gtgactgtct
      721 acagaagaca tggacgggca tatgttccca tcgcacaagt gaaagatgtg tacgtggtaa
      781 cagatcagat tcctgtgttt gtgactatgt tccagaagaa cgatcgaaat tcatccgacg
      841 aaaccttcct caaagatctc cccattatgt ttgatgtcct gattcatgat cctagccact
      901 tcctcaatta ttctaccatt aactacaagt ggagcttcgg ggataatact ggcctgtttg
      961 tttccaccaa tcatactgtg aatcacacgt atgtgctcaa tggaaccttc agccttaacc
     1021 tcactgtgaa agctgcagca ccaggacctt gtccgccacc gccaccacca cccagacctt
     1081 caaaacccac cccttcttta ggacctgctg gtgacaaccc cctggagctg agtaggattc
     1141 ctgatgaaaa ctgccagatt aacagatatg gccactttca agccaccatc acaattgtag
     1201 agggaatctt agaggttaac atcatccaga tgacagacgt cctgatgccg gtgccatggc
     1261 ctgaaagctc cctaatagac tttgtcgtga cctgccaagg gagcattccc acggaggtct
     1321 gtaccatcat ttctgacccc acctgcgaga tcacccagaa cacagtctgc agccctgtgg
     1381 atgtggatga gatgtgtctg ctgactgtga gacgaacctt caatgggtct gggacgtact
     1441 gtgtgaacct caccctgggg gatgacacaa gcctggctct cacgagcacc ctgatttctg
     1501 ttcctgacag agacccagcc tcgcctttaa ggatggcaaa cagtgccctg atctccgttg
     1561 gctgcttggc catatttgtc actgtgatct ccctcttggt gtacaaaaaa cacaaggaat
     1621 acaacccaat agaaaatagt cctgggaatg tggtcagaag caaaggcctg agtgtctttc
     1681 tcaaccgtgc aaaagccgtg ttcttcccgg gaaaccagga aaaggatccg ctactcaaaa
     1741 accaagaatt taaaggagtt tcttaaattt cgaccttgtt tctgaagctc acttttcagt
     1801 gccattgatg tgagatgtgc tggagtggct attaaccttt ttttcctaaa gattattgtt
     1861 aaatagatat tgtggtttgg ggaagttgaa ttttttatag gttaaatgtc attttagaga
     1921 tggggagagg gattatactg caggcagctt cagccatgtt gtgaaactga taaaagcaac
     1981 ttagcaaggc ttcttttcat tattttttat gtttcactta taaagtctta ggtaactagt
     2041 aggatagaaa cactgtgtcc cgagagtaag gagagaagct actattgatt agagcctaac
     2101 ccaggttaac tgcaagaaga ggcgggatac tttcagcttt ccatgtaact gtatgcataa
     2161 agccaatgta gtccagtttc taagatcatg ttccaagcta actgaatccc acttcaatac
     2221 acactcatga actcctgatg gaacaataac aggcccaagc ctgtggtatg atgtgcacac
     2281 ttgctagact cagaaaaaat actactctca taaatgggtg ggagtatttt ggtgacaacc
     2341 tactttgctt ggctgagtga aggaatgata ttcatatatt catttattcc atggacattt
     2401 agttagtgct ttttatatac caggcatgat gctgagtgac actcttgtgt atatttccaa
     2461 atttttgtac agtcgctgca catatttgaa atcatatatt aagactttcc aaagatgagg
     2521 tccctggttt ttcatggcaa cttgatcagt aaggatttca cctctgtttg taactaaaac
     2581 catctactat atgttagaca tgacattctt tttctctcct tcctgaaaaa taaagtgtgg
     2641 gaagagacaa
//
