LOCUS       NM_016301               1457 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens GPN-loop GTPase 3 (GPN3), transcript variant 1, mRNA.
ACCESSION   NM_016301
VERSION     NM_016301.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1457)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   2  (bases 1 to 1457)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 1457)
  AUTHORS   Lara-Chacon,B., Guerrero-Rodriguez,S.L., Ramirez-Hernandez,K.J.,
            Robledo-Rivera,A.Y., Velazquez,M.A.V., Sanchez-Olea,R. and
            Calera,M.R.
  TITLE     Gpn3 Is Essential for Cell Proliferation of Breast Cancer Cells
            Independent of Their Malignancy Degree
  JOURNAL   Technol Cancer Res Treat 18, 1533033819870823 (2019)
   PUBMED   31431135
  REMARK    GeneRIF: our analysis of public data revealed that Gpn3
            overexpression was associated with a significant decrease in
            overall survival in patients with estrogen receptor-positive and
            Human epidermal growth factor receptor 2 (HER2+) breast cancer,
            supporting our proposal that targeting Gpn3 could potentially
            benefit patients with breast cancer.
REFERENCE   4  (bases 1 to 1457)
  AUTHORS   Mendez-Hernandez,L.E., Robledo-Rivera,A.Y., Macias-Silva,M.,
            Calera,M.R. and Sanchez-Olea,R.
  TITLE     Gpn3 is polyubiquitinated on lysine 216 and degraded by the
            proteasome in the cell nucleus in a Gpn1-inhibitable manner
  JOURNAL   FEBS Lett 591 (22), 3757-3770 (2017)
   PUBMED   29029378
  REMARK    GeneRIF: Results indicate that polyubiquitination on lysine 216 is
            a regulatory process for proteasomal degradation of Gpn3 that is
            not in a complex with Gpn1, a critical partner of Gpn3 in human
            cells.
REFERENCE   5  (bases 1 to 1457)
  AUTHORS   Barbosa-Camacho,A.A., Mendez-Hernandez,L.E., Lara-Chacon,B.,
            Pena-Gomez,S.G., Romero,V., Gonzalez-Gonzalez,R.,
            Guerra-Moreno,J.A., Robledo-Rivera,A.Y., Sanchez-Olea,R. and
            Calera,M.R.
  TITLE     The Gpn3 Q279* cancer-associated mutant inhibits Gpn1 nuclear
            export and is deficient in RNA polymerase II nuclear targeting
  JOURNAL   FEBS Lett 591 (21), 3555-3566 (2017)
   PUBMED   28940195
  REMARK    GeneRIF: an acquired PDZ-binding motif in Gpn3 Q279* caused Gpn3
            nuclear entry, and inhibited Gpn1 nuclear export and Gpn3-mediated
            RNAPII nuclear targeting.
REFERENCE   6  (bases 1 to 1457)
  AUTHORS   Mendez-Hernandez,L.E., Perez-Mejia,A.E., Lara-Chacon,B.,
            Barbosa-Camacho,A.A., Pena-Gomez,S.G., Martinez-Sanchez,M.,
            Robledo-Rivera,A.Y., Sanchez-Olea,R. and Calera,M.R.
  TITLE     Gpn1 and Gpn3 associate tightly and their protein levels are
            mutually dependent in mammalian cells
  JOURNAL   FEBS Lett 588 (21), 3823-3829 (2014)
   PUBMED   25241168
  REMARK    GeneRIF: Gpn1-Gpn3 interaction was essential to maintain
            steady-state protein levels of both GTPases
REFERENCE   7  (bases 1 to 1457)
  AUTHORS   Calera,M.R., Zamora-Ramos,C., Araiza-Villanueva,M.G.,
            Moreno-Aguilar,C.A., Pena-Gomez,S.G., Castellanos-Teran,F.,
            Robledo-Rivera,A.Y. and Sanchez-Olea,R.
  TITLE     Parcs/Gpn3 is required for the nuclear accumulation of RNA
            polymerase II
  JOURNAL   Biochim Biophys Acta 1813 (10), 1708-1716 (2011)
   PUBMED   21782856
  REMARK    GeneRIF: Parcs/Gpn3 plays a critical role in the nuclear
            accumulation of RNAP II, and this function explains the relative
            importance of Parcs/Gpn3 in cell proliferation.
REFERENCE   8  (bases 1 to 1457)
  AUTHORS   Carre,C. and Shiekhattar,R.
  TITLE     Human GTPases associate with RNA polymerase II to mediate its
            nuclear import
  JOURNAL   Mol Cell Biol 31 (19), 3953-3962 (2011)
   PUBMED   21768307
  REMARK    GeneRIF: Data show that GPN1/GPN3 define a new family of small
            GTPases that are specialized for the transport of RNA polymerase II
            into the nucleus.
REFERENCE   9  (bases 1 to 1457)
  AUTHORS   Clark,H.F., Gurney,A.L., Abaya,E., Baker,K., Baldwin,D., Brush,J.,
            Chen,J., Chow,B., Chui,C., Crowley,C., Currell,B., Deuel,B.,
            Dowd,P., Eaton,D., Foster,J., Grimaldi,C., Gu,Q., Hass,P.E.,
            Heldens,S., Huang,A., Kim,H.S., Klimowski,L., Jin,Y., Johnson,S.,
            Lee,J., Lewis,L., Liao,D., Mark,M., Robbie,E., Sanchez,C.,
            Schoenfeld,J., Seshagiri,S., Simmons,L., Singh,J., Smith,V.,
            Stinson,J., Vagts,A., Vandlen,R., Watanabe,C., Wieand,D., Woods,K.,
            Xie,M.H., Yansura,D., Yi,S., Yu,G., Yuan,J., Zhang,M., Zhang,Z.,
            Goddard,A., Wood,W.I., Godowski,P. and Gray,A.
  TITLE     The secreted protein discovery initiative (SPDI), a large-scale
            effort to identify novel human secreted and transmembrane proteins:
            a bioinformatics assessment
  JOURNAL   Genome Res 13 (10), 2265-2270 (2003)
   PUBMED   12975309
  REMARK    Erratum:[Genome Res. 2003 Dec;13(12):2759]
REFERENCE   10 (bases 1 to 1457)
  AUTHORS   Simpson,J.C., Wellenreuther,R., Poustka,A., Pepperkok,R. and
            Wiemann,S.
  TITLE     Systematic subcellular localization of novel proteins identified by
            large-scale cDNA sequencing
  JOURNAL   EMBO Rep 1 (3), 287-292 (2000)
   PUBMED   11256614
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BC008416.1 and AK223209.1.
            
            On Nov 22, 2018 this sequence version replaced NM_016301.3.
            
            Transcript Variant: This variant (1) encodes the predominant
            isoform (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC008416.1, SRR3476690.1100503.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000228827.8/ ENSP00000228827.3
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-784               BC008416.1         3-786
            785-1391            AK223209.1         784-1390
            1392-1457           BC008416.1         1394-1459
FEATURES             Location/Qualifiers
     source          1..1457
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12q24.11"
     gene            1..1457
                     /gene="GPN3"
                     /gene_synonym="ATPBD1C"
                     /note="GPN-loop GTPase 3"
                     /db_xref="GeneID:51184"
                     /db_xref="HGNC:HGNC:30186"
     exon            1..102
                     /gene="GPN3"
                     /gene_synonym="ATPBD1C"
                     /inference="alignment:Splign:2.1.0"
     CDS             55..909
                     /gene="GPN3"
                     /gene_synonym="ATPBD1C"
                     /note="isoform 1 is encoded by transcript variant 1;
                     protein x 0004; ATP-binding domain 1 family member C"
                     /codon_start=1
                     /product="GPN-loop GTPase 3 isoform 1"
                     /protein_id="NP_057385.3"
                     /db_xref="CCDS:CCDS9147.1"
                     /db_xref="GeneID:51184"
                     /db_xref="HGNC:HGNC:30186"
                     /translation="MPRYAQLVMGPAGSGKSTYCATMVQHCEALNRSVQVVNLDPAAE
                     HFNYSVMADIRELIEVDDVMEDDSLRFGPNGGLVFCMEYFANNFDWLENCLGHVEDDY
                     ILFDCPGQIELYTHLPVMKQLVQQLEQWEFRVCGVFLVDSQFMVESFKFISGILAALS
                     AMISLEIPQVNIMTKMDLLSKKAKKEIEKFLDPDMYSLLEDSTSDLRSKKFKKLTKAI
                     CGLIDDYSMVRFLPYDQSDEESMNIVLQHIDFAIQYGEDLEFKEPKEREDESSSMFDE
                     YFQECQDE"
     exon            103..211
                     /gene="GPN3"
                     /gene_synonym="ATPBD1C"
                     /inference="alignment:Splign:2.1.0"
     exon            212..379
                     /gene="GPN3"
                     /gene_synonym="ATPBD1C"
                     /inference="alignment:Splign:2.1.0"
     exon            380..504
                     /gene="GPN3"
                     /gene_synonym="ATPBD1C"
                     /inference="alignment:Splign:2.1.0"
     exon            505..620
                     /gene="GPN3"
                     /gene_synonym="ATPBD1C"
                     /inference="alignment:Splign:2.1.0"
     exon            621..717
                     /gene="GPN3"
                     /gene_synonym="ATPBD1C"
                     /inference="alignment:Splign:2.1.0"
     exon            718..846
                     /gene="GPN3"
                     /gene_synonym="ATPBD1C"
                     /inference="alignment:Splign:2.1.0"
     exon            847..1457
                     /gene="GPN3"
                     /gene_synonym="ATPBD1C"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1016..1021
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GPN3"
                     /gene_synonym="ATPBD1C"
                     /note="hexamer: AGTAAA"
     polyA_site      1043
                     /gene="GPN3"
                     /gene_synonym="ATPBD1C"
                     /note="major polyA site"
     regulatory      1370..1375
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GPN3"
                     /gene_synonym="ATPBD1C"
                     /note="hexamer: AGTAAA"
     polyA_site      1393
                     /gene="GPN3"
                     /gene_synonym="ATPBD1C"
     regulatory      1417..1422
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GPN3"
                     /gene_synonym="ATPBD1C"
                     /note="hexamer: AACAAA"
     polyA_site      1457
                     /gene="GPN3"
                     /gene_synonym="ATPBD1C"
ORIGIN      
        1 agtgggcgtc gcgcgaaggc taagggagtg tggcgggcgg ctccgggagc caacatgcct
       61 cggtatgcgc agctggtcat gggccccgcg ggcagcggga agagcaccta ctgtgccacc
      121 atggtccagc actgtgaagc cctcaaccgg tctgtccaag ttgtaaacct ggatccagca
      181 gcagaacact tcaactactc cgtgatggct gacatccggg aactgatcga ggtggatgat
      241 gtaatggagg atgattctct gcgattcggt cccaacggag gattggtatt ttgcatggag
      301 tactttgcca ataattttga ctggctggag aactgtcttg gccatgtaga ggacgactat
      361 atcctttttg attgtccagg tcagattgag ttgtacactc acctgcctgt gatgaaacag
      421 ctggtccagc agctcgagca gtgggagttc cgagtctgtg gagtttttct tgttgattct
      481 cagttcatgg tggagtcatt caagtttatt tctggcatct tggcagccct gagtgccatg
      541 atctctctag aaattccgca agtcaacatc atgacaaaaa tggatctgct gagtaaaaaa
      601 gcaaaaaagg aaattgagaa atttttagat ccagacatgt attctttatt agaagattct
      661 acaagtgact taagaagcaa aaaattcaag aaactgacta aagctatatg tggactgatt
      721 gatgactaca gcatggttcg atttttacct tacgatcagt cagatgaaga aagcatgaac
      781 attgtattgc agcatattga ttttgccatt caatatggag aagacctaga atttaaagaa
      841 ccaaaggaac gtgaagatga gtcttcctct atgtttgacg aatattttca agaatgccag
      901 gatgaatgaa gagtttacta aaagtaacca tctaaagagc ttgtggccaa accagcagaa
      961 cattcttctc ttcaaaggat gcaatagtag aaagctactt attttaatga aaaaaagtaa
     1021 aacttcgttc tttatcagcc tcatgcctga atcaaatttt taattattct gaaactgctg
     1081 ctgtttaaag tggaatcttt tagtattata acagcatcac tttagatttt gtaagtcaaa
     1141 attgaaatga atgcacatag atttatatat aaattagcac ctgagctaag gttaaggctg
     1201 gtctaaactt attttcactt tttgtattat ttttgagatg caggaattac tgtaacaaaa
     1261 tatgtatgtc cgaagggaaa aagctgcaag gatatatata agaccactgc ttatctgtat
     1321 cttcccattt tcctatattg aaaatgtata ttatttatat aacttaaaaa gtaaaaataa
     1381 ctatgttttg agatatgtat gtgtatatat aaaagaaaca aaggttttta atgattcttg
     1441 gacctagata acaagta
//
