LOCUS       NM_007266               2445 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens GPN-loop GTPase 1 (GPN1), transcript variant 1, mRNA.
ACCESSION   NM_007266
VERSION     NM_007266.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   2  (bases 1 to 2445)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 2445)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 2445)
  AUTHORS   Mendez-Hernandez,L.E., Robledo-Rivera,A.Y., Macias-Silva,M.,
            Calera,M.R. and Sanchez-Olea,R.
  TITLE     Gpn3 is polyubiquitinated on lysine 216 and degraded by the
            proteasome in the cell nucleus in a Gpn1-inhibitable manner
  JOURNAL   FEBS Lett 591 (22), 3757-3770 (2017)
   PUBMED   29029378
  REMARK    GeneRIF: Results show that Gpn1-inhibitable, nuclear
            polyubiquitination on lysine 216 regulates the half-life of Gpn3 by
            tagging it for proteasomal degradation.
REFERENCE   5  (bases 1 to 2445)
  AUTHORS   Reyes-Pardo,H., Barbosa-Camacho,A.A., Perez-Mejia,A.E.,
            Lara-Chacon,B., Salas-Estrada,L.A., Robledo-Rivera,A.Y.,
            Montero-Moran,G.M., Lara-Gonzalez,S., Calera,M.R. and
            Sanchez-Olea,R.
  TITLE     A nuclear export sequence in GPN-loop GTPase 1, an essential
            protein for nuclear targeting of RNA polymerase II, is necessary
            and sufficient for nuclear export
  JOURNAL   Biochim Biophys Acta 1823 (10), 1756-1766 (2012)
   PUBMED   22796641
  REMARK    GeneRIF: A highly active, evolutionarily conserved nuclear export
            sequences in XAB1/Gpn1, was identified.
REFERENCE   6  (bases 1 to 2445)
  AUTHORS   Carre,C. and Shiekhattar,R.
  TITLE     Human GTPases associate with RNA polymerase II to mediate its
            nuclear import
  JOURNAL   Mol Cell Biol 31 (19), 3953-3962 (2011)
   PUBMED   21768307
  REMARK    GeneRIF: Data show that GPN1/GPN3 define a new family of small
            GTPases that are specialized for the transport of RNA polymerase II
            into the nucleus.
REFERENCE   7  (bases 1 to 2445)
  AUTHORS   Kraja,A.T., Vaidya,D., Pankow,J.S., Goodarzi,M.O., Assimes,T.L.,
            Kullo,I.J., Sovio,U., Mathias,R.A., Sun,Y.V., Franceschini,N.,
            Absher,D., Li,G., Zhang,Q., Feitosa,M.F., Glazer,N.L.,
            Haritunians,T., Hartikainen,A.L., Knowles,J.W., North,K.E.,
            Iribarren,C., Kral,B., Yanek,L., O'Reilly,P.F., McCarthy,M.I.,
            Jaquish,C., Couper,D.J., Chakravarti,A., Psaty,B.M., Becker,L.C.,
            Province,M.A., Boerwinkle,E., Quertermous,T., Palotie,L.,
            Jarvelin,M.R., Becker,D.M., Kardia,S.L., Rotter,J.I., Chen,Y.D. and
            Borecki,I.B.
  TITLE     A bivariate genome-wide approach to metabolic syndrome: STAMPEED
            consortium
  JOURNAL   Diabetes 60 (4), 1329-1339 (2011)
   PUBMED   21386085
REFERENCE   8  (bases 1 to 2445)
  AUTHORS   Forget,D., Lacombe,A.A., Cloutier,P., Al-Khoury,R., Bouchard,A.,
            Lavallee-Adam,M., Faubert,D., Jeronimo,C., Blanchette,M. and
            Coulombe,B.
  TITLE     The protein interaction network of the human transcription
            machinery reveals a role for the conserved GTPase RPAP4/GPN1 and
            microtubule assembly in nuclear import and biogenesis of RNA
            polymerase II
  JOURNAL   Mol Cell Proteomics 9 (12), 2827-2839 (2010)
   PUBMED   20855544
  REMARK    GeneRIF: RPAP4/GPN1 is a member of a newly discovered GTPase family
            that contains a highly conserved GPN loop motif that is essential,
            along with its GTP-binding motifs, for nuclear localization of
            POLR2A/RPB1 in a process that also requires microtubule assembly
REFERENCE   9  (bases 1 to 2445)
  AUTHORS   Lembo,F., Pero,R., Angrisano,T., Vitiello,C., Iuliano,R.,
            Bruni,C.B. and Chiariotti,L.
  TITLE     MBDin, a novel MBD2-interacting protein, relieves MBD2 repression
            potential and reactivates transcription from methylated promoters
  JOURNAL   Mol Cell Biol 23 (5), 1656-1665 (2003)
   PUBMED   12588985
  REMARK    GeneRIF: relieves MBD2-mediated transcriptional repression and
            reactivates transcription from methylated promoters
REFERENCE   10 (bases 1 to 2445)
  AUTHORS   Nitta,M., Saijo,M., Kodo,N., Matsuda,T., Nakatsu,Y., Tamai,H. and
            Tanaka,K.
  TITLE     A novel cytoplasmic GTPase XAB1 interacts with DNA repair protein
            XPA
  JOURNAL   Nucleic Acids Res 28 (21), 4212-4218 (2000)
   PUBMED   11058119
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK298866.1, BC007451.2,
            AJ010842.1 and AC074091.6.
            
            On Aug 31, 2018 this sequence version replaced NM_007266.3.
            
            Summary: This gene encodes a guanosine triphosphatase enzyme. The
            encoded protein may play a role in DNA repair and may function in
            activation of transcription. Alternatively spliced transcript
            variants have been described. [provided by RefSeq, Feb 2009].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660809.128275.1,
                                           SRR1803614.247358.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000610189.2/ ENSP00000476446.1
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2                 AK298866.1         55-56
            3-1392              BC007451.2         1-1390
            1393-1780           AJ010842.1         1337-1724
            1781-2445           AC074091.6         128722-129386       c
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2p23.3"
     gene            1..2445
                     /gene="GPN1"
                     /gene_synonym="ATPBD1A; MBDIN; NTPBP; RPAP4; XAB1"
                     /note="GPN-loop GTPase 1"
                     /db_xref="GeneID:11321"
                     /db_xref="HGNC:HGNC:17030"
                     /db_xref="MIM:611479"
     exon            1..120
                     /gene="GPN1"
                     /gene_synonym="ATPBD1A; MBDIN; NTPBP; RPAP4; XAB1"
                     /inference="alignment:Splign:2.1.0"
     CDS             10..1134
                     /gene="GPN1"
                     /gene_synonym="ATPBD1A; MBDIN; NTPBP; RPAP4; XAB1"
                     /note="isoform a is encoded by transcript variant 1;
                     MBD2-interacting protein; ATP(GTP)-binding protein; XPA
                     binding protein 1, GTPase; RNA polymerase II associated
                     protein 4; RNAPII-associated protein 4"
                     /codon_start=1
                     /product="GPN-loop GTPase 1 isoform a"
                     /protein_id="NP_009197.3"
                     /db_xref="CCDS:CCDS1760.3"
                     /db_xref="GeneID:11321"
                     /db_xref="HGNC:HGNC:17030"
                     /db_xref="MIM:611479"
                     /translation="MAASAAAAELQASGGPRHPVCLLVLGMAGSGKTTFVQRLTGHLH
                     AQGTPPYVINLDPAVHEVPFPANIDIRDTVKYKEVMKQYGLGPNGGIVTSLNLFATRF
                     DQVMKFIEKAQNMSKYVLIDTPGQIEVFTWSASGTIITEALASSFPTVVIYVMDTSRS
                     TNPVTFMSNMLYACSILYKTKLPFIVVMNKTDIIDHSFAVEWMQDFEAFQDALNQETT
                     YVSNLTRSMSLVLDEFYSSLRVVGVSAVLGTGLDELFVQVTSAAEEYEREYRPEYERL
                     KKSLANAESQQQREQLERLRKDMGSVALDAGTAKDSLSPVLHPSDLILTRGTLDEEDE
                     EADSDTDDIDHRVTEESHEEPAFQNFMQESMAQYWKRNNK"
     exon            121..214
                     /gene="GPN1"
                     /gene_synonym="ATPBD1A; MBDIN; NTPBP; RPAP4; XAB1"
                     /inference="alignment:Splign:2.1.0"
     exon            215..254
                     /gene="GPN1"
                     /gene_synonym="ATPBD1A; MBDIN; NTPBP; RPAP4; XAB1"
                     /inference="alignment:Splign:2.1.0"
     exon            255..321
                     /gene="GPN1"
                     /gene_synonym="ATPBD1A; MBDIN; NTPBP; RPAP4; XAB1"
                     /inference="alignment:Splign:2.1.0"
     exon            322..359
                     /gene="GPN1"
                     /gene_synonym="ATPBD1A; MBDIN; NTPBP; RPAP4; XAB1"
                     /inference="alignment:Splign:2.1.0"
     exon            360..438
                     /gene="GPN1"
                     /gene_synonym="ATPBD1A; MBDIN; NTPBP; RPAP4; XAB1"
                     /inference="alignment:Splign:2.1.0"
     exon            439..533
                     /gene="GPN1"
                     /gene_synonym="ATPBD1A; MBDIN; NTPBP; RPAP4; XAB1"
                     /inference="alignment:Splign:2.1.0"
     exon            534..579
                     /gene="GPN1"
                     /gene_synonym="ATPBD1A; MBDIN; NTPBP; RPAP4; XAB1"
                     /inference="alignment:Splign:2.1.0"
     exon            580..726
                     /gene="GPN1"
                     /gene_synonym="ATPBD1A; MBDIN; NTPBP; RPAP4; XAB1"
                     /inference="alignment:Splign:2.1.0"
     exon            727..809
                     /gene="GPN1"
                     /gene_synonym="ATPBD1A; MBDIN; NTPBP; RPAP4; XAB1"
                     /inference="alignment:Splign:2.1.0"
     exon            810..849
                     /gene="GPN1"
                     /gene_synonym="ATPBD1A; MBDIN; NTPBP; RPAP4; XAB1"
                     /inference="alignment:Splign:2.1.0"
     exon            850..940
                     /gene="GPN1"
                     /gene_synonym="ATPBD1A; MBDIN; NTPBP; RPAP4; XAB1"
                     /inference="alignment:Splign:2.1.0"
     exon            941..1048
                     /gene="GPN1"
                     /gene_synonym="ATPBD1A; MBDIN; NTPBP; RPAP4; XAB1"
                     /inference="alignment:Splign:2.1.0"
     exon            1049..2445
                     /gene="GPN1"
                     /gene_synonym="ATPBD1A; MBDIN; NTPBP; RPAP4; XAB1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1759..1764
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GPN1"
                     /gene_synonym="ATPBD1A; MBDIN; NTPBP; RPAP4; XAB1"
                     /note="hexamer: GATAAA"
     polyA_site      1780
                     /gene="GPN1"
                     /gene_synonym="ATPBD1A; MBDIN; NTPBP; RPAP4; XAB1"
                     /note="major polyA site"
     regulatory      2421..2426
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GPN1"
                     /gene_synonym="ATPBD1A; MBDIN; NTPBP; RPAP4; XAB1"
                     /note="hexamer: AATAAA"
     polyA_site      2445
                     /gene="GPN1"
                     /gene_synonym="ATPBD1A; MBDIN; NTPBP; RPAP4; XAB1"
ORIGIN      
        1 aggaggaaga tggcggcgtc cgcagctgcc gctgagctcc aggcttctgg gggtccgcgg
       61 cacccagtgt gtctgttggt gttgggaatg gcgggatccg ggaaaaccac ttttgtacag
      121 aggctcacag gacacctgca tgcccaaggc actccaccgt atgtgatcaa cctggatcca
      181 gcagtacatg aagttccctt tcctgccaat attgatattc gtgatactgt aaagtataaa
      241 gaagtaatga aacaatatgg acttggaccc aatggcggca tagtgacctc actcaatctc
      301 tttgctacca gatttgatca ggtgatgaaa tttattgaga aggcccagaa catgtccaaa
      361 tatgtgttga ttgacacacc tggacagatt gaggtattca cctggtcagc ttctgggaca
      421 attatcactg aagcccttgc atcctcattt ccaacagttg tcatctatgt aatggacaca
      481 tcgagaagta ccaacccagt gaccttcatg tccaacatgc tctatgcctg cagcatctta
      541 tacaaaacca agctgccttt cattgtggtc atgaataaaa ctgacatcat tgaccacagc
      601 tttgcagtgg aatggatgca ggattttgag gctttccaag atgccttgaa tcaagagact
      661 acatacgtca gtaacctgac tcgttcaatg agcctggtgt tagatgagtt ttacagctca
      721 ctcagggtgg tgggtgtctc tgctgttctg ggtactggat tagatgaact ctttgtgcaa
      781 gttaccagtg ctgccgaaga atatgaaagg gagtatcgtc ctgaatatga acgtctgaaa
      841 aaatcactgg ccaacgcaga gagccaacag cagagagaac aactggaacg ccttcgaaaa
      901 gatatgggtt ctgtagcctt ggatgcaggg actgccaaag acagcttatc tcctgtgctg
      961 cacccttctg atttgatcct gactcgagga accttggatg aagaggatga ggaagcagac
     1021 agcgatactg atgacattga ccacagagtt acagaggaaa gccatgaaga gccagcattc
     1081 cagaatttta tgcaagaatc gatggcacaa tactggaaga gaaacaataa ataggagact
     1141 ttagcacact tcacttgttt ctagaagtcc agaattttgg acctccacgt gaaagaactg
     1201 ttcttacctc tgaactgggg gctcccataa gggataattt tcctcagagt agcaaagttt
     1261 ctcttattag agaaatcttg tgactcagat gaagtcaggg atagaagacc cttggacctg
     1321 gcaggttaat gctgattatt ccttggcctt tcccttgtat ttatgcaagg aaggatatac
     1381 tgagctgata ctcttccaag cctacaactt caagttttat catttgaact caagtacttt
     1441 tgctgctgag gaatggaatc aaaagaacgt agtctcctgg tgaccacctc agatctctat
     1501 tattaggcta gatgtatagc ctctactccc ccagcttctt gctcttgacc ctgcactgta
     1561 agttgccctt ctattagcag ccaaggaaaa gggaaacatg agcttatcca gaacggtggc
     1621 agagtctcct tggcaatcaa ccaacgttgc tatgaaatat gcctcacact gtatagctca
     1681 ttataggacg tcaggtttgt tgaaaaaagt gggcaagaca tgattaatga atcagaatcc
     1741 tgtttcattg gtgacttgga taaagacttt ttaattttaa ctttgttcta agactgcttg
     1801 tcatgatttc aaattagaaa attatataat tgcaaacagc ttcacttctc ctgttcaaca
     1861 gaggcttaag gccagatgtc caaacttgtc tcaataagga ggtgatattt tactaaagtt
     1921 tcccacgtgc acatactgac taaatacaga gctaggccca gtttgtattg tactctgaac
     1981 ttaatgcaaa gtctccttgg tgattttcgc aaagtccgtg gatttgggtc agaggcacat
     2041 ttcatacata acagccctta taaacgtttg ccctgcctcc acattttaca gtatcttaaa
     2101 acagtacatt tctttcaaag aattttatct ctatgagtca gtactccaac ttagtggttc
     2161 tcacattgtg gaccaggagc atcagcatca tctggggaat tgttcaactt acctcttata
     2221 acctatgaac tcagaaaccc tgggggtggg ctgagcagtc tgttttagaa agccctccat
     2281 gtgattctga tgcatagtag cctatgacat aattccagac caggtgaatc tcaagatact
     2341 aatcctcaca tcatttgcct tctcatattt ccctcgtctt gaccatctgg tgcctgttat
     2401 gcagtttaac attctgcagc aataaaagtg ttttattata aagta
//
