LOCUS       NM_001503               5790 bp    mRNA    linear   PRI 01-AUG-2024
DEFINITION  Homo sapiens glycosylphosphatidylinositol specific phospholipase D1
            (GPLD1), mRNA.
ACCESSION   NM_001503
VERSION     NM_001503.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5790)
  AUTHORS   Cao,J., Zhou,A., Zhou,Z., Liu,H. and Jia,S.
  TITLE     The role of GPLD1 in chronic diseases
  JOURNAL   J Cell Physiol 238 (7), 1407-1415 (2023)
   PUBMED   37393554
  REMARK    GeneRIF: The role of GPLD1 in chronic diseases.
            Review article
REFERENCE   2  (bases 1 to 5790)
  AUTHORS   Kano,T., Tsumagari,R., Nakashima,A., Kikkawa,U., Ueda,S.,
            Yamanoue,M., Takei,N. and Shirai,Y.
  TITLE     RalA, PLD and mTORC1 Are Required for Kinase-Independent Pathways
            in DGKbeta-Induced Neurite Outgrowth
  JOURNAL   Biomolecules 11 (12), 1814 (2021)
   PUBMED   34944458
  REMARK    GeneRIF: RalA, PLD and mTORC1 Are Required for Kinase-Independent
            Pathways in DGKbeta-Induced Neurite Outgrowth.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5790)
  AUTHORS   Masuda,S., Fujishima,Y., Maeda,N., Tsugawa-Shimizu,Y., Nakamura,Y.,
            Tanaka,Y., Obata,Y., Fukuda,S., Nagao,H., Kita,S., Nishizawa,H. and
            Shimomura,I.
  TITLE     Impact of glycosylphosphatidylinositol-specific phospholipase D on
            hepatic diacylglycerol accumulation, steatosis, and insulin
            resistance in diet-induced obesity
  JOURNAL   Am J Physiol Endocrinol Metab 316 (2), E239-E250 (2019)
   PUBMED   30457913
  REMARK    GeneRIF: Serum GPI-PLD in the NAFLD (+) group was significantly
            higher than in the NAFLD (-) group in men with metabolic syndrome.
REFERENCE   4  (bases 1 to 5790)
  AUTHORS   Chakraborti,S., Sarkar,J., Bhuyan,R. and Chakraborti,T.
  TITLE     Role of curcumin in PLD activation by Arf6-cytohesin1 signaling
            axis in U46619-stimulated pulmonary artery smooth muscle cells
  JOURNAL   Mol Cell Biochem 438 (1-2), 97-109 (2018)
   PUBMED   28780751
  REMARK    GeneRIF: computational study suggests that although curcumin to
            some extent binds with Tp receptor, yet the inhibition of Arf6GDP
            to Arf6GTP conversion appeared to be an important mechanism by
            which curcumin inhibits U46619-induced increase in PLD activity in
            PASMCs.
REFERENCE   5  (bases 1 to 5790)
  AUTHORS   von Toerne,C., Huth,C., de Las Heras Gala,T., Kronenberg,F.,
            Herder,C., Koenig,W., Meisinger,C., Rathmann,W., Waldenberger,M.,
            Roden,M., Peters,A., Thorand,B. and Hauck,S.M.
  TITLE     MASP1, THBS1, GPLD1 and ApoA-IV are novel biomarkers associated
            with prediabetes: the KORA F4 study
  JOURNAL   Diabetologia 59 (9), 1882-1892 (2016)
   PUBMED   27344311
  REMARK    GeneRIF: Study discovered novel and independent associations of
            prediabetes and related traits with MASP1, and some evidence for
            associations with THBS1, GPLD1 and ApoA-IV, suggesting a role for
            these proteins in the pathophysiology of type 2 diabetes.
REFERENCE   6  (bases 1 to 5790)
  AUTHORS   Metz,C.N., Brunner,G., Choi-Muira,N.H., Nguyen,H., Gabrilove,J.,
            Caras,I.W., Altszuler,N., Rifkin,D.B., Wilson,E.L. and Davitz,M.A.
  TITLE     Release of GPI-anchored membrane proteins by a cell-associated
            GPI-specific phospholipase D
  JOURNAL   EMBO J 13 (7), 1741-1751 (1994)
   PUBMED   7512501
REFERENCE   7  (bases 1 to 5790)
  AUTHORS   Hoener,M.C. and Brodbeck,U.
  TITLE     Phosphatidylinositol-glycan-specific phospholipase D is an
            amphiphilic glycoprotein that in serum is associated with
            high-density lipoproteins
  JOURNAL   Eur J Biochem 206 (3), 747-757 (1992)
   PUBMED   1606959
REFERENCE   8  (bases 1 to 5790)
  AUTHORS   Metz,C.N., Zhang,Y.Y., Guo,Y., Tsang,T.C., Kochan,J.P.,
            Altszuler,N. and Davitz,M.A.
  TITLE     Production of the glycosylphosphatidylinositol-specific
            phospholipase D by the islets of Langerhans
  JOURNAL   J Biol Chem 266 (27), 17733-17736 (1991)
   PUBMED   1833386
REFERENCE   9  (bases 1 to 5790)
  AUTHORS   Scallon,B.J., Fung,W.J., Tsang,T.C., Li,S., Kado-Fong,H.,
            Huang,K.S. and Kochan,J.P.
  TITLE     Primary structure and functional activity of a
            phosphatidylinositol-glycan-specific phospholipase D
  JOURNAL   Science 252 (5004), 446-448 (1991)
   PUBMED   2017684
REFERENCE   10 (bases 1 to 5790)
  AUTHORS   Davitz,M.A., Hom,J. and Schenkman,S.
  TITLE     Purification of a glycosyl-phosphatidylinositol-specific
            phospholipase D from human plasma
  JOURNAL   J Biol Chem 264 (23), 13760-13764 (1989)
   PUBMED   2760042
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AJ308108.1, AL031230.1,
            L11702.1, AY007546.2, AL359713.25 and BU587813.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_001503.3.
            
            Summary: Many proteins are tethered to the extracellular face of
            eukaryotic plasma membranes by a glycosylphosphatidylinositol (GPI)
            anchor. The GPI-anchor is a glycolipid found on many blood cells.
            The protein encoded by this gene is a GPI degrading enzyme.
            Glycosylphosphatidylinositol specific phospholipase D1 hydrolyzes
            the inositol phosphate linkage in proteins anchored by
            phosphatidylinositol glycans, thereby releasing the attached
            protein from the plasma membrane. [provided by RefSeq, Jul 2008].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: L11702.1, AJ308108.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000230036.2/ ENSP00000230036.1
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-65                AJ308108.1         45-109
            66-67               AL031230.1         33165-33166         c
            68-863              L11702.1           1-796
            864-2159            AY007546.2         802-2097
            2160-2160           AL359713.25        133736-133736       c
            2161-2788           AJ308108.1         2205-2832
            2789-5451           AL359713.25        122690-125352       c
            5452-5787           BU587813.1         3-338
            5788-5790           BU587813.1         341-343
FEATURES             Location/Qualifiers
     source          1..5790
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6p22.3"
     gene            1..5790
                     /gene="GPLD1"
                     /gene_synonym="GPIPLD; GPIPLDM; PIGPLD; PIGPLD1; PLD"
                     /note="glycosylphosphatidylinositol specific phospholipase
                     D1"
                     /db_xref="GeneID:2822"
                     /db_xref="HGNC:HGNC:4459"
                     /db_xref="MIM:602515"
     exon            1..164
                     /gene="GPLD1"
                     /gene_synonym="GPIPLD; GPIPLDM; PIGPLD; PIGPLD1; PLD"
                     /inference="alignment:Splign:2.1.0"
     CDS             68..2590
                     /gene="GPLD1"
                     /gene_synonym="GPIPLD; GPIPLDM; PIGPLD; PIGPLD1; PLD"
                     /EC_number="3.1.4.50"
                     /note="glycoprotein phospholipase D; GPI-specific
                     phospholipase D; phosphatidylinositol-glycan-specific
                     phospholipase D; GPI-PLD; PI-G PLD"
                     /codon_start=1
                     /product="phosphatidylinositol-glycan-specific
                     phospholipase D precursor"
                     /protein_id="NP_001494.2"
                     /db_xref="CCDS:CCDS4553.1"
                     /db_xref="GeneID:2822"
                     /db_xref="HGNC:HGNC:4459"
                     /db_xref="MIM:602515"
                     /translation="MSAFRLWPGLLIMLGSLCHRGSPCGLSTHVEIGHRALEFLQLHN
                     GRVNYRELLLEHQDAYQAGIVFPDCFYPSICKGGKFHDVSESTHWTPFLNASVHYIRE
                     NYPLPWEKDTEKLVAFLFGITSHMAADVSWHSLGLEQGFLRTMGAIDFHGSYSEAHSA
                     GDFGGDVLSQFEFNFNYLARRWYVPVKDLLGIYEKLYGRKVITENVIVDCSHIQFLEM
                     YGEMLAVSKLYPTYSTKSPFLVEQFQEYFLGGLDDMAFWSTNIYHLTSFMLENGTSDC
                     NLPENPLFIACGGQQNHTQGSKMQKNDFHRNLTTSLTESVDRNINYTERGVFFSVNSW
                     TPDSMSFIYKALERNIRTMFIGGSQLSQKHVSSPLASYFLSFPYARLGWAMTSADLNQ
                     DGHGDLVVGAPGYSRPGHIHIGRVYLIYGNDLGLPPVDLDLDKEAHRILEGFQPSGRF
                     GSALAVLDFNVDGVPDLAVGAPSVGSEQLTYKGAVYVYFGSKQGGMSSSPNITISCQD
                     IYCNLGWTLLAADVNGDSEPDLVIGSPFAPGGGKQKGIVAAFYSGPSLSDKEKLNVEA
                     ANWTVRGEEDFSWFGYSLHGVTVDNRTLLLVGSPTWKNASRLGHLLHIRDEKKSLGRV
                     YGYFPPNGQSWFTISGDKAMGKLGTSLSSGHVLMNGTLKQVLLVGAPTYDDVSKVAFL
                     TVTLHQGGATRMYALTSDAQPLLLSTFSGDRRFSRFGGVLHLSDLDDDGLDEIIMAAP
                     LRIADVTSGLIGGEDGRVYVYNGKETTLGDMTGKCKSWITPCPEEKAQYVLISPEASS
                     RFGSSLITVRSKAKNQVVIAAGRSSLGARLSGALHVYSLGSD"
     exon            165..220
                     /gene="GPLD1"
                     /gene_synonym="GPIPLD; GPIPLDM; PIGPLD; PIGPLD1; PLD"
                     /inference="alignment:Splign:2.1.0"
     exon            221..299
                     /gene="GPLD1"
                     /gene_synonym="GPIPLD; GPIPLDM; PIGPLD; PIGPLD1; PLD"
                     /inference="alignment:Splign:2.1.0"
     exon            300..397
                     /gene="GPLD1"
                     /gene_synonym="GPIPLD; GPIPLDM; PIGPLD; PIGPLD1; PLD"
                     /inference="alignment:Splign:2.1.0"
     exon            398..508
                     /gene="GPLD1"
                     /gene_synonym="GPIPLD; GPIPLDM; PIGPLD; PIGPLD1; PLD"
                     /inference="alignment:Splign:2.1.0"
     exon            509..557
                     /gene="GPLD1"
                     /gene_synonym="GPIPLD; GPIPLDM; PIGPLD; PIGPLD1; PLD"
                     /inference="alignment:Splign:2.1.0"
     exon            558..612
                     /gene="GPLD1"
                     /gene_synonym="GPIPLD; GPIPLDM; PIGPLD; PIGPLD1; PLD"
                     /inference="alignment:Splign:2.1.0"
     exon            613..720
                     /gene="GPLD1"
                     /gene_synonym="GPIPLD; GPIPLDM; PIGPLD; PIGPLD1; PLD"
                     /inference="alignment:Splign:2.1.0"
     exon            721..748
                     /gene="GPLD1"
                     /gene_synonym="GPIPLD; GPIPLDM; PIGPLD; PIGPLD1; PLD"
                     /inference="alignment:Splign:2.1.0"
     exon            749..888
                     /gene="GPLD1"
                     /gene_synonym="GPIPLD; GPIPLDM; PIGPLD; PIGPLD1; PLD"
                     /inference="alignment:Splign:2.1.0"
     exon            889..954
                     /gene="GPLD1"
                     /gene_synonym="GPIPLD; GPIPLDM; PIGPLD; PIGPLD1; PLD"
                     /inference="alignment:Splign:2.1.0"
     exon            955..1075
                     /gene="GPLD1"
                     /gene_synonym="GPIPLD; GPIPLDM; PIGPLD; PIGPLD1; PLD"
                     /inference="alignment:Splign:2.1.0"
     exon            1076..1215
                     /gene="GPLD1"
                     /gene_synonym="GPIPLD; GPIPLDM; PIGPLD; PIGPLD1; PLD"
                     /inference="alignment:Splign:2.1.0"
     exon            1216..1402
                     /gene="GPLD1"
                     /gene_synonym="GPIPLD; GPIPLDM; PIGPLD; PIGPLD1; PLD"
                     /inference="alignment:Splign:2.1.0"
     exon            1403..1513
                     /gene="GPLD1"
                     /gene_synonym="GPIPLD; GPIPLDM; PIGPLD; PIGPLD1; PLD"
                     /inference="alignment:Splign:2.1.0"
     exon            1514..1588
                     /gene="GPLD1"
                     /gene_synonym="GPIPLD; GPIPLDM; PIGPLD; PIGPLD1; PLD"
                     /inference="alignment:Splign:2.1.0"
     exon            1589..1745
                     /gene="GPLD1"
                     /gene_synonym="GPIPLD; GPIPLDM; PIGPLD; PIGPLD1; PLD"
                     /inference="alignment:Splign:2.1.0"
     exon            1746..1887
                     /gene="GPLD1"
                     /gene_synonym="GPIPLD; GPIPLDM; PIGPLD; PIGPLD1; PLD"
                     /inference="alignment:Splign:2.1.0"
     exon            1888..1993
                     /gene="GPLD1"
                     /gene_synonym="GPIPLD; GPIPLDM; PIGPLD; PIGPLD1; PLD"
                     /inference="alignment:Splign:2.1.0"
     exon            1994..2087
                     /gene="GPLD1"
                     /gene_synonym="GPIPLD; GPIPLDM; PIGPLD; PIGPLD1; PLD"
                     /inference="alignment:Splign:2.1.0"
     exon            2088..2264
                     /gene="GPLD1"
                     /gene_synonym="GPIPLD; GPIPLDM; PIGPLD; PIGPLD1; PLD"
                     /inference="alignment:Splign:2.1.0"
     exon            2265..2425
                     /gene="GPLD1"
                     /gene_synonym="GPIPLD; GPIPLDM; PIGPLD; PIGPLD1; PLD"
                     /inference="alignment:Splign:2.1.0"
     exon            2426..2452
                     /gene="GPLD1"
                     /gene_synonym="GPIPLD; GPIPLDM; PIGPLD; PIGPLD1; PLD"
                     /inference="alignment:Splign:2.1.0"
     exon            2453..2503
                     /gene="GPLD1"
                     /gene_synonym="GPIPLD; GPIPLDM; PIGPLD; PIGPLD1; PLD"
                     /inference="alignment:Splign:2.1.0"
     exon            2504..5790
                     /gene="GPLD1"
                     /gene_synonym="GPIPLD; GPIPLDM; PIGPLD; PIGPLD1; PLD"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5767..5772
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GPLD1"
                     /gene_synonym="GPIPLD; GPIPLDM; PIGPLD; PIGPLD1; PLD"
                     /note="hexamer: AATAAA"
     polyA_site      5790
                     /gene="GPLD1"
                     /gene_synonym="GPIPLD; GPIPLDM; PIGPLD; PIGPLD1; PLD"
                     /note="major polyA site"
ORIGIN      
        1 gagtcccagt gctgctgcag ctctgagcat tcccacgtca ccagagaagc cggtgggcaa
       61 tgagatcatg tctgctttca ggttgtggcc tggcctgctg atcatgttgg gttctctctg
      121 ccatagaggt tcaccgtgtg gcctttcaac acacgtagaa ataggacaca gagctctgga
      181 gtttcttcag cttcacaatg ggcgtgttaa ctacagagag ctgttactag aacaccagga
      241 tgcgtatcag gctggaatcg tgtttcctga ttgtttttac cctagcatct gcaaaggagg
      301 aaaattccat gatgtgtctg agagcactca ctggactccg tttcttaatg caagcgttca
      361 ttatatccga gagaactatc cccttccctg ggagaaggac acagagaaac tggtagcttt
      421 cttgtttgga attacttctc acatggcggc agatgtcagc tggcatagtc tgggccttga
      481 acaaggattc cttaggacca tgggagctat tgattttcac ggctcctatt cagaggctca
      541 ttcggctggt gattttggag gagatgtgtt gagccagttt gaatttaatt ttaattacct
      601 tgcacgacgc tggtatgtgc cagtcaaaga tctactggga atttatgaga aactgtatgg
      661 tcgaaaagtc atcaccgaaa atgtaatcgt tgattgttca catatccagt tcttagaaat
      721 gtatggtgag atgctagctg tttccaagtt atatcccact tactctacaa agtccccgtt
      781 tttggtggaa caattccaag agtattttct tggaggactg gatgatatgg cattttggtc
      841 cactaatatt taccatctaa caagcttcat gttggagaat gggaccagtg actgcaacct
      901 gcctgagaac cctctgttca ttgcatgtgg cggccagcaa aaccacaccc agggctcaaa
      961 aatgcagaaa aatgattttc acagaaattt gactacatcc ctaactgaaa gtgttgacag
     1021 gaatataaac tatactgaaa gaggagtgtt ctttagtgta aattcctgga ccccggattc
     1081 catgtccttt atctacaagg ctttggaaag gaacataagg acaatgttca taggtggctc
     1141 tcagttgtca caaaagcacg tctccagccc cttagcatct tacttcttgt catttcctta
     1201 tgcgaggctt ggctgggcaa tgacctcagc tgacctcaac caggatgggc acggtgacct
     1261 cgtggtgggc gcaccaggct acagccgccc cggccacatc cacatcgggc gcgtgtacct
     1321 catctacggc aatgacctgg gcctgccacc tgttgacctg gacctggaca aggaggccca
     1381 caggatcctt gaaggcttcc agccctcagg tcggtttggc tcggccttgg ctgtgttgga
     1441 ctttaacgtg gacggcgtgc ctgacctggc cgtgggagct ccctcggtgg gctccgagca
     1501 gctcacctac aaaggtgccg tgtatgtcta ctttggttcc aaacaaggag gaatgtcttc
     1561 ttcccctaac atcaccattt cttgccagga catctactgt aacttgggct ggactctctt
     1621 ggctgcagat gtgaatggag acagtgaacc cgatctggtc atcggctccc cttttgcacc
     1681 aggtggaggg aagcagaagg gaattgtggc tgcgttttat tctggcccca gcctgagcga
     1741 caaagaaaaa ctgaacgtgg aggcagccaa ctggacggtg agaggcgagg aagacttctc
     1801 ctggtttgga tattcccttc acggtgtcac tgtggacaac agaaccttgc tgttggttgg
     1861 gagcccgacc tggaagaatg ccagcaggct gggccatttg ttacacatcc gagatgagaa
     1921 aaagagcctt gggagggtgt atggctactt cccaccaaac ggccaaagct ggtttaccat
     1981 ttctggagac aaggcaatgg ggaaactggg tacttccctt tccagtggcc acgtactgat
     2041 gaatgggact ctgaaacaag tgctgctggt tggagcccct acgtacgatg acgtgtctaa
     2101 ggtggcattc ctgaccgtga ccctacacca aggcggagcc actcgcatgt acgcactcac
     2161 atctgacgcg cagcctctgc tgctcagcac cttcagcgga gaccgccgct tctcccgatt
     2221 tggtggcgtt ctgcacttga gtgacctgga tgatgatggc ttagatgaaa tcatcatggc
     2281 agcccccctg aggatagcag atgtaacctc tggactgatt gggggagaag acggccgagt
     2341 atatgtatat aatggcaaag agaccaccct tggtgacatg actggcaaat gcaaatcatg
     2401 gataactcca tgtccagaag aaaaggccca atatgtattg atttctcctg aagccagctc
     2461 aaggtttggg agctccctca tcaccgtgag gtccaaggca aagaaccaag tcgtcattgc
     2521 tgctggaagg agttctttgg gagcccgact ctccggggca cttcacgtct atagccttgg
     2581 ctcagattga agatttcact gcatttcccc actctgccca cctctctcat gctgaatcac
     2641 atccatggtg agcattttga tggacaaagt ggcacatcca gtggagcggt ggtagatcct
     2701 gatagacatg gggctcctgg gagtagagag acacactaac agccacaccc tctggaaatc
     2761 tgatacagta aatatatgac tgcaccagaa atatgtgaaa tagcagacat tctgcttact
     2821 catgtctcct tccacagttt acttcctcgc tccctttgca tctaaacctt tcttctttcc
     2881 caacttattg cctgtagtca gacctgctgt acaacctatt tcctcttcct cttgaatgtc
     2941 tttccaatgg ctggaaaggt ccctctgtgg ttatctgtta gaacagtctc tgtacacaat
     3001 tcctcctaaa aacatccttt tttaaaaaaa gaattgttca gccataaaga aagaacaaga
     3061 tcatgccctt tgcagggaca tggatggagc tggaggccat tatccttcat aaactattgc
     3121 aggaacagaa aaccaaacac tccatattct cacttgtaag tgggagctaa atgagaacac
     3181 gtggacacat agagggaaac aacacacact ggggcctatg agagggcgga aggtgggagg
     3241 agggagagat caggaaaaat aactaatgga tacttagggt gatgaaataa tctgtgtaac
     3301 aaacccccat gacacacctt tatgtatgta acaaaccagc acttcctgcg catgtacccc
     3361 tgaacttaaa agttaaaaaa aagttgaact taaaaataac agattggccc atgccaatca
     3421 aagtataata gaaagcatag tatacagaaa aaaaaaaaaa aaagaaactg agggaggaaa
     3481 aatagtacag agtaaagcat gggggtgagg gataacctca cattccaaat cacttgtgac
     3541 ttagtccttt ttaactttta ttttaggttc agtggtacat gtgaaggttt tatcttttct
     3601 gttcctccca ccttccccac tcaagtagac cccagtgtgt gttgttttgt gttcataagt
     3661 tcttttaact tagcacccac ttgtgagaac atgctatact tggtatttgg ttttctgttc
     3721 ctggtcgtgt gttagtttgc taaggatgat agtccttttt tttttttttt ttttttgaga
     3781 cggagtcttg ctctgttgcc caggctggag tgcagtgatg tgatctcagc tcactgcaac
     3841 ctccgcctcc caggttcaag tgattctcct gtctcagcct cccaggcagc tgggactaca
     3901 ggtgcacaat actacgcccg gctaattttt gtatttttag tagagacagg gtttcaccat
     3961 gttggccagg ctggtctcga actcctgatc tcaggtgatc cacccacctt gacctcccaa
     4021 agtgctggga ttacatgtgt gagccacagg acctggccga taatcctttc ttaatgagga
     4081 ttaatacctt ttcaatgttt atatagtgaa ggacaaatat aaaaaccaag actatcaccc
     4141 ttctgtgccc tccaatactt ttgtagagtt ggctgttcta acacctaaag agcctagcga
     4201 cactgctagt tttaagccat ctcatgaggg acctttgtcg cattaggtta caacttgtgc
     4261 caaaagaact agagggaaaa gatgtggcct gagccacacg ctactgtaag ggccggagag
     4321 gaaagcaggc cttggcctcg cttgttcctg ggctgttttt cgtaggatgg atttcatttg
     4381 tcagtcctgc agcgtggcac acttaaggga atggaatgta tcatagggtt tcagtttcag
     4441 taaacattag cgggcattat ctgtaaacag taactagttt attttgtgtt aaattgggaa
     4501 gaacctcttt cttctgccac gggtgagatg atactaaaag caggagaaaa taacaaatga
     4561 gaagagccct tctcttctca gcttctctca agagtagaga ctggtgaaaa ggggacagga
     4621 agaggaatcc agctgaagag tggaaagtta acaagaacat caagaggtac atccctagag
     4681 agcatctctg ggtcactccc aggatgacct catttttctg gaagaggtac atgtgctagc
     4741 tgcatagtaa cctatggcac agcaaaagca caaggaagcg ccatttggtt ttgtttaggg
     4801 acaattattg gtagcccaac ctatctgaag aattactttt aattaaaagg atggttattt
     4861 ttaggggacc agaaattact tagttgtatt cacttatgag ttgagcagtt agagcactct
     4921 gacatctgac acttgagtaa agggatgttt ttctcagtac tgggtctttt cattagacgg
     4981 atgcatttat atagtgtcag tttttatgct tcactaaatg ataaggcctc tgtttaaaca
     5041 tgaccattat ttacctcacg atttatattt ttctcaatgc ctgaatacac ccaatagcaa
     5101 gcaccaggtg gaggttaaga cccacaaaaa tggaagcaag cttcaagttt ataggacatt
     5161 taaccatact aaccccaatg tcaaaaacaa tgtaaattat actcctactg taaaaggtca
     5221 actgatatgg cacgtatgta aaaaacctgg catacaggaa aaaattaatg catgacagtc
     5281 taatatatta tcctgataca tggattaaat tttctttcca ctaagtatta actatagttt
     5341 taagcattga ttctgcttaa taactaaaat gtaaagacct taccttgtag ggttgtaatt
     5401 tctcattaac agaaatctga acttatacaa gtttaactga aatttatctt tatttcaaga
     5461 tatcagaaga catttacata tttgcatttt ctgtcttgct actatctgcg tatatgtaac
     5521 agcttttctc aaggcttcaa gataggaatt gttttatgcc tttgtatcac agacctaaga
     5581 gaccaaaatt atttgaagat ggagtgggga caaaactagg gtctctgcag tccctactcc
     5641 atgtaccatc cattagctca cctctgtagg tattactaaa gtcatgagag ttaaatccaa
     5701 cgtgcaactt taatattgca attccaaaaa ataaagctgt atacaactct tggaataact
     5761 caccaaaata aatagctttt taatataaaa
//
