LOCUS       NM_145171                599 bp    mRNA    linear   PRI 11-FEB-2025
DEFINITION  Homo sapiens glycoprotein hormone subunit beta 5 (GPHB5), mRNA.
ACCESSION   NM_145171
VERSION     NM_145171.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 599)
  AUTHORS   Wang,Y., Xiang,T., Xia,X., Zhang,H., Geng,S., Yang,G., Qiu,S.,
            He,Y., Liu,R., Li,L., Liu,H., Li,K., Zhang,L., Liang,Z. and He,J.
  TITLE     Elevated circulating GPHB5 levels in women with insulin resistance
            and polycystic ovary syndrome: A cross-sectional study and multiple
            intervention studies
  JOURNAL   Front Endocrinol (Lausanne) 13, 1010714 (2022)
   PUBMED   36568071
  REMARK    GeneRIF: Elevated circulating GPHB5 levels in women with insulin
            resistance and polycystic ovary syndrome: A cross-sectional study
            and multiple intervention studies.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 599)
  AUTHORS   Xiang,T., Zhang,S., Li,Q., Li,L., Liu,H., Chen,C., Yang,G. and
            Yang,M.
  TITLE     GPHB5 Is a Biomarker in Women With Metabolic Syndrome: Results From
            Cross-Sectional and Intervention Studies
  JOURNAL   Front Endocrinol (Lausanne) 13, 893142 (2022)
   PUBMED   35757403
  REMARK    GeneRIF: GPHB5 Is a Biomarker in Women With Metabolic Syndrome:
            Results From Cross-Sectional and Intervention Studies.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 599)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 599)
  AUTHORS   Suzuki,C., Nagasaki,H., Okajima,Y., Suga,H., Ozaki,N., Arima,H.,
            Iwasaki,Y. and Oiso,Y.
  TITLE     Inflammatory cytokines regulate glycoprotein subunit beta5 of
            thyrostimulin through nuclear factor-kappaB
  JOURNAL   Endocrinology 150 (5), 2237-2243 (2009)
   PUBMED   19095738
  REMARK    GeneRIF: Inflammatory cytokines positively regulate thyrostimulin
            through NF-kappaB activation.
REFERENCE   5  (bases 1 to 599)
  AUTHORS   Okajima,Y., Nagasaki,H., Suzuki,C., Suga,H., Ozaki,N., Arima,H.,
            Hamada,Y., Civelli,O. and Oiso,Y.
  TITLE     Biochemical roles of the oligosaccharide chains in thyrostimulin, a
            heterodimeric hormone of glycoprotein hormone subunits alpha 2
            (GPA2) and beta 5 (GPB5)
  JOURNAL   Regul Pept 148 (1-3), 62-67 (2008)
   PUBMED   18433898
  REMARK    GeneRIF: Dual site-disrupted GPA2 and the GPB5 mutant were not
            expressed in either the conditioned medium or cell lysate.
REFERENCE   6  (bases 1 to 599)
  AUTHORS   Okada,S.L., Ellsworth,J.L., Durnam,D.M., Haugen,H.S.,
            Holloway,J.L., Kelley,M.L., Lewis,K.E., Ren,H., Sheppard,P.O.,
            Storey,H.M., Waggie,K.S., Wolf,A.C., Yao,L.Y. and Webster,P.J.
  TITLE     A glycoprotein hormone expressed in corticotrophs exhibits unique
            binding properties on thyroid-stimulating hormone receptor
  JOURNAL   Mol Endocrinol 20 (2), 414-425 (2006)
   PUBMED   16210345
REFERENCE   7  (bases 1 to 599)
  AUTHORS   Sudo,S., Kuwabara,Y., Park,J.I., Hsu,S.Y. and Hsueh,A.J.
  TITLE     Heterodimeric fly glycoprotein hormone-alpha2 (GPA2) and
            glycoprotein hormone-beta5 (GPB5) activate fly leucine-rich
            repeat-containing G protein-coupled receptor-1 (DLGR1) and
            stimulation of human thyrotropin receptors by chimeric fly GPA2 and
            human GPB5
  JOURNAL   Endocrinology 146 (8), 3596-3604 (2005)
   PUBMED   15890769
  REMARK    GeneRIF: Srosophila GPA2/GPB5 heterodimers did not activate human
            TSH or gonadotropin receptors, chimeric fly GPA2/human GPB5
            heterodimers stimulated human TSH receptors. (GPA2 in drosophila)
REFERENCE   8  (bases 1 to 599)
  AUTHORS   Heilig,R., Eckenberg,R., Petit,J.L., Fonknechten,N., Da Silva,C.,
            Cattolico,L., Levy,M., Barbe,V., de Berardinis,V., Ureta-Vidal,A.,
            Pelletier,E., Vico,V., Anthouard,V., Rowen,L., Madan,A., Qin,S.,
            Sun,H., Du,H., Pepin,K., Artiguenave,F., Robert,C., Cruaud,C.,
            Bruls,T., Jaillon,O., Friedlander,L., Samson,G., Brottier,P.,
            Cure,S., Segurens,B., Aniere,F., Samain,S., Crespeau,H., Abbasi,N.,
            Aiach,N., Boscus,D., Dickhoff,R., Dors,M., Dubois,I., Friedman,C.,
            Gouyvenoux,M., James,R., Madan,A., Mairey-Estrada,B., Mangenot,S.,
            Martins,N., Menard,M., Oztas,S., Ratcliffe,A., Shaffer,T.,
            Trask,B., Vacherie,B., Bellemere,C., Belser,C., Besnard-Gonnet,M.,
            Bartol-Mavel,D., Boutard,M., Briez-Silla,S., Combette,S.,
            Dufosse-Laurent,V., Ferron,C., Lechaplais,C., Louesse,C.,
            Muselet,D., Magdelenat,G., Pateau,E., Petit,E.,
            Sirvain-Trukniewicz,P., Trybou,A., Vega-Czarny,N., Bataille,E.,
            Bluet,E., Bordelais,I., Dubois,M., Dumont,C., Guerin,T.,
            Haffray,S., Hammadi,R., Muanga,J., Pellouin,V., Robert,D.,
            Wunderle,E., Gauguet,G., Roy,A., Sainte-Marthe,L., Verdier,J.,
            Verdier-Discala,C., Hillier,L., Fulton,L., McPherson,J.,
            Matsuda,F., Wilson,R., Scarpelli,C., Gyapay,G., Wincker,P.,
            Saurin,W., Quetier,F., Waterston,R., Hood,L. and Weissenbach,J.
  TITLE     The DNA sequence and analysis of human chromosome 14
  JOURNAL   Nature 421 (6923), 601-607 (2003)
   PUBMED   12508121
  REMARK    Erratum:[Nature. 2023 Aug;620(7973):E17. doi:
            10.1038/s41586-023-06403-y.. PMID: 37491470]
REFERENCE   9  (bases 1 to 599)
  AUTHORS   Hsu,S.Y., Nakabayashi,K. and Bhalla,A.
  TITLE     Evolution of glycoprotein hormone subunit genes in bilateral
            metazoa: identification of two novel human glycoprotein hormone
            subunit family genes, GPA2 and GPB5
  JOURNAL   Mol Endocrinol 16 (7), 1538-1551 (2002)
   PUBMED   12089349
  REMARK    GeneRIF: identification and characterization of GPB5 genes
REFERENCE   10 (bases 1 to 599)
  AUTHORS   Nakabayashi,K., Matsumi,H., Bhalla,A., Bae,J., Mosselman,S.,
            Hsu,S.Y. and Hsueh,A.J.
  TITLE     Thyrostimulin, a heterodimer of two new human glycoprotein hormone
            subunits, activates the thyroid-stimulating hormone receptor
  JOURNAL   J Clin Invest 109 (11), 1445-1452 (2002)
   PUBMED   12045258
  REMARK    GeneRIF: Thyrostimulin, a heterodimer of two new human glycoprotein
            hormone subunits, activates the thyroid-stimulating hormone
            receptor (thyrostimulin)
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL118555.6 and BM709443.1.
            
            On May 17, 2019 this sequence version replaced NM_145171.3.
            
            Summary: GPHB5 is a cystine knot-forming polypeptide and a subunit
            of the dimeric glycoprotein hormone family (Hsu et al., 2002
            [PubMed 12089349]).[supplied by OMIM, Mar 2008].
            
            ##Evidence-Data-START##
            Transcript exon combination :: BM709443.1 [ECO:0000332]
            RNAseq introns              :: partial sample support SAMEA1965299,
                                           SAMEA1968968 [ECO:0000350]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000621500.2/ ENSP00000478993.1
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-56                AL118555.6         111830-111885
            57-599              BM709443.1         1-543
FEATURES             Location/Qualifiers
     source          1..599
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="14"
                     /map="14q23.2"
     gene            1..599
                     /gene="GPHB5"
                     /gene_synonym="B5; GPB5; ZLUT1"
                     /note="glycoprotein hormone subunit beta 5"
                     /db_xref="GeneID:122876"
                     /db_xref="HGNC:HGNC:18055"
                     /db_xref="MIM:609652"
     exon            1..112
                     /gene="GPHB5"
                     /gene_synonym="B5; GPB5; ZLUT1"
                     /inference="alignment:Splign:2.1.0"
     exon            113..317
                     /gene="GPHB5"
                     /gene_synonym="B5; GPB5; ZLUT1"
                     /inference="alignment:Splign:2.1.0"
     CDS             114..506
                     /gene="GPHB5"
                     /gene_synonym="B5; GPB5; ZLUT1"
                     /note="glycoprotein beta 5; glycoprotein hormone beta
                     subunit; thyrostimulin subunit beta; glycoprotein hormone
                     beta 5"
                     /codon_start=1
                     /product="glycoprotein hormone beta-5 precursor"
                     /protein_id="NP_660154.3"
                     /db_xref="CCDS:CCDS73643.1"
                     /db_xref="GeneID:122876"
                     /db_xref="HGNC:HGNC:18055"
                     /db_xref="MIM:609652"
                     /translation="MKLAFLFLGPMALLLLAGYGCVLGASSGNLRTFVGCAVREFTFL
                     AKKPGCRGLRITTDACWGRCETWEKPILEPPYIEAHHRVCTYNETKQVTVKLPNCAPG
                     VDPFYTYPVAIRCDCGACSTATTECETI"
     exon            318..599
                     /gene="GPHB5"
                     /gene_synonym="B5; GPB5; ZLUT1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 aacagaaggt agagacattt acccagggca aacttctacc attcattgtg acttcctgaa
       61 atcttagtgc aagtttcagc tctaaaagaa gagtgggctc ctgcaagatt agcatgaagc
      121 tggcattcct cttccttggc cccatggccc tcctccttct ggctggctat ggctgtgtcc
      181 tcggtgcctc cagtgggaac ctgcgcacct ttgtgggctg tgccgtgagg gagtttactt
      241 tcctggccaa gaagccaggc tgcaggggcc ttcggatcac cacggatgcc tgctggggtc
      301 gctgtgagac ctgggagaaa cccattctgg aaccccccta tattgaagcc catcatcgag
      361 tctgtaccta caacgagacc aaacaggtga ctgtcaagct gcccaactgt gccccgggag
      421 tcgacccctt ctacacctat cccgtggcca tccgctgtga ctgcggagcc tgctccactg
      481 ccaccacgga gtgtgagacc atctgaggcc gctagctgct ctctgcagac ccacctgtgt
      541 gagcagcaca tgcagttata cttcctggat gcaagactgt ttaatttcga ccacaccca
//
