LOCUS       NM_000408               5812 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens glycerol-3-phosphate dehydrogenase 2 (GPD2),
            transcript variant 2, mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_000408
VERSION     NM_000408.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5812)
  AUTHORS   Oh,S., Mai,X.L., Kim,J., de Guzman,A.C.V., Lee,J.Y. and Park,S.
  TITLE     Glycerol 3-phosphate dehydrogenases (1 and 2) in cancer and other
            diseases
  JOURNAL   Exp Mol Med 56 (5), 1066-1079 (2024)
   PUBMED   38689091
  REMARK    GeneRIF: Glycerol 3-phosphate dehydrogenases (1 and 2) in cancer
            and other diseases.
            Review article
REFERENCE   2  (bases 1 to 5812)
  AUTHORS   Mikeli,M., Fujikawa,M. and Tanabe,T.
  TITLE     GPD2: The relationship with cancer and neural stemness
  JOURNAL   Cells Dev 173, 203824 (2023)
   PUBMED   36592694
  REMARK    GeneRIF: GPD2: The relationship with cancer and neural stemness.
REFERENCE   3  (bases 1 to 5812)
  AUTHORS   Wu,S., Mao,C., Kondiparthi,L., Poyurovsky,M.V., Olszewski,K. and
            Gan,B.
  TITLE     A ferroptosis defense mechanism mediated by glycerol-3-phosphate
            dehydrogenase 2 in mitochondria
  JOURNAL   Proc Natl Acad Sci U S A 119 (26), e2121987119 (2022)
   PUBMED   35749365
  REMARK    GeneRIF: A ferroptosis defense mechanism mediated by
            glycerol-3-phosphate dehydrogenase 2 in mitochondria.
REFERENCE   4  (bases 1 to 5812)
  AUTHORS   Morgenstern,M., Peikert,C.D., Lubbert,P., Suppanz,I., Klemm,C.,
            Alka,O., Steiert,C., Naumenko,N., Schendzielorz,A., Melchionda,L.,
            Muhlhauser,W.W.D., Knapp,B., Busch,J.D., Stiller,S.B.,
            Dannenmaier,S., Lindau,C., Licheva,M., Eickhorst,C., Galbusera,R.,
            Zerbes,R.M., Ryan,M.T., Kraft,C., Kozjak-Pavlovic,V., Drepper,F.,
            Dennerlein,S., Oeljeklaus,S., Pfanner,N., Wiedemann,N. and
            Warscheid,B.
  TITLE     Quantitative high-confidence human mitochondrial proteome and its
            dynamics in cellular context
  JOURNAL   Cell Metab 33 (12), 2464-2483 (2021)
   PUBMED   34800366
REFERENCE   5  (bases 1 to 5812)
  AUTHORS   Li,X., Zhou,L., Zhang,Y., He,X., Lu,H., Zhang,L., Tian,Y., Liu,X.,
            Zheng,H., Shao,J. and Long,M.
  TITLE     mGPDH Deficiency leads to melanoma metastasis via induced NRF2
  JOURNAL   J Cell Mol Med 25 (11), 5305-5315 (2021)
   PUBMED   33939274
  REMARK    GeneRIF: mGPDH Deficiency leads to melanoma metastasis via induced
            NRF2.
REFERENCE   6  (bases 1 to 5812)
  AUTHORS   Vertessy,B.G., Orosz,F. and Ovadi,J.
  TITLE     Modulation of the interaction between aldolase and
            glycerol-phosphate dehydrogenase by fructose phosphates
  JOURNAL   Biochim Biophys Acta 1078 (2), 236-242 (1991)
   PUBMED   2065091
REFERENCE   7  (bases 1 to 5812)
  AUTHORS   Zolnierowicz,S., Swierczynski,J. and Zelewski,L.
  TITLE     Isolation and properties of glycerol-3-phosphate oxidoreductase
            from human placenta
  JOURNAL   Eur J Biochem 154 (1), 161-166 (1986)
   PUBMED   3943520
REFERENCE   8  (bases 1 to 5812)
  AUTHORS   Ovadi,J., Mohamed Osman,I.R. and Batke,J.
  TITLE     Interaction of the dissociable glycerol-3-phosphate dehydrogenase
            and fructose-1,6-bisphosphate aldolase. Quantitative analysis by an
            extrinsic fluorescence probe
  JOURNAL   Eur J Biochem 133 (2), 433-437 (1983)
   PUBMED   6406231
REFERENCE   9  (bases 1 to 5812)
  AUTHORS   MacDonald,M.J.
  TITLE     High content of mitochondrial glycerol-3-phosphate dehydrogenase in
            pancreatic islets and its inhibition by diazoxide
  JOURNAL   J Biol Chem 256 (16), 8287-8290 (1981)
   PUBMED   6790537
REFERENCE   10 (bases 1 to 5812)
  AUTHORS   Harding,J.W. Jr., Pyeritz,E.A., Copeland,E.S. and White,H.B. 3rd.
  TITLE     Role of glycerol 3-phosphate dehydrogenase in glyceride metabolism.
            Effect of diet on enzyme activities in chicken liver
  JOURNAL   Biochem J 146 (1), 223-229 (1975)
   PUBMED   167714
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC092686.3 and AC011308.8.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_000408.4.
            
            Summary: The protein encoded by this gene localizes to the inner
            mitochondrial membrane and catalyzes the conversion of
            glycerol-3-phosphate to dihydroxyacetone phosphate, using FAD as a
            cofactor. Along with GDP1, the encoded protein constitutes the
            glycerol phosphate shuttle, which reoxidizes NADH formed during
            glycolysis. Two transcript variants encoding the same protein have
            been found for this gene.[provided by RefSeq, Jan 2010].
            
            Transcript Variant: This variant (2) differs in the 5' UTR compared
            to variant 1. Both variants 1 and 2 encode the same protein.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803615.230556.1,
                                           SRR1660803.138469.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            MANE Ensembl match                 :: ENST00000438166.7/
                                                  ENSP00000409708.2
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-135               AC092686.3         64793-64927
            136-245             AC092686.3         104512-104621
            246-417             AC011308.8         8703-8874
            418-542             AC011308.8         23455-23579
            543-640             AC011308.8         24879-24976
            641-804             AC011308.8         25992-26155
            805-969             AC011308.8         62267-62431
            970-1114            AC011308.8         63261-63405
            1115-1308           AC011308.8         70048-70241
            1309-1443           AC011308.8         81484-81618
            1444-1619           AC011308.8         82022-82197
            1620-1751           AC011308.8         82746-82877
            1752-1910           AC011308.8         83793-83951
            1911-2023           AC011308.8         91548-91660
            2024-2102           AC011308.8         91745-91823
            2103-2201           AC011308.8         92349-92447
            2202-5812           AC011308.8         95452-99062
FEATURES             Location/Qualifiers
     source          1..5812
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q24.1"
     gene            1..5812
                     /gene="GPD2"
                     /gene_synonym="GDH2; GPDM; mGDH; mGPDH"
                     /note="glycerol-3-phosphate dehydrogenase 2"
                     /db_xref="GeneID:2820"
                     /db_xref="HGNC:HGNC:4456"
                     /db_xref="MIM:138430"
     exon            1..135
                     /gene="GPD2"
                     /gene_synonym="GDH2; GPDM; mGDH; mGPDH"
                     /inference="alignment:Splign:2.1.0"
     exon            136..245
                     /gene="GPD2"
                     /gene_synonym="GDH2; GPDM; mGDH; mGPDH"
                     /inference="alignment:Splign:2.1.0"
     CDS             144..2327
                     /gene="GPD2"
                     /gene_synonym="GDH2; GPDM; mGDH; mGPDH"
                     /EC_number="1.1.5.3"
                     /note="glycerol-3-phosphate dehydrogenase, mitochondrial;
                     mitochondrial glycerophosphate dehydrogenase; GPD-M;
                     mtGPD; GPDH-M; glycerol-3-phosphate dehydrogenase 2
                     (mitochondrial); testicular tissue protein Li 76;
                     mitohondrial glycerophosphate dehydrogenase"
                     /codon_start=1
                     /product="glycerol-3-phosphate dehydrogenase,
                     mitochondrial precursor"
                     /protein_id="NP_000399.3"
                     /db_xref="CCDS:CCDS2202.1"
                     /db_xref="GeneID:2820"
                     /db_xref="HGNC:HGNC:4456"
                     /db_xref="MIM:138430"
                     /translation="MAFQKAVKGTILVGGGALATVLGLSQFAHYRRKQMNLAYVKAAD
                     CISEPVNREPPSREAQLLTLQNTSEFDILVIGGGATGSGCALDAVTRGLKTALVERDD
                     FSSGTSSRSTKLIHGGVRYLQKAIMKLDIEQYRMVKEALHERANLLEIAPHLSAPLPI
                     MLPVYKWWQLPYYWVGIKLYDLVAGSNCLKSSYVLSKSRALEHFPMLQKDKLVGAIVY
                     YDGQHNDARMNLAIALTAARYGAATANYMEVVSLLKKTDPQTGKVRVSGARCKDVLTG
                     QEFDVRAKCVINATGPFTDSVRKMDDKDAAAICQPSAGVHIVMPGYYSPESMGLLDPA
                     TSDGRVIFFLPWQKMTIAGTTDTPTDVTHHPIPSEEDINFILNEVRNYLSCDVEVRRG
                     DVLAAWSGIRPLVTDPKSADTQSISRNHVVDISESGLITIAGGKWTTYRSMAEDTINA
                     AVKTHNLKAGPSRTVGLFLQGGKDWSPTLYIRLVQDYGLESEVAQHLAATYGDKAFEV
                     AKMASVTGKRWPIVGVRLVSEFPYIEAEVKYGIKEYACTAVDMISRRTRLAFLNVQAA
                     EEALPRIVELMGRELNWDDYKKQEQLETARKFLYYEMGYKSRSEQLTDRSEISLLPSD
                     IDRYKKRFHKFDADQKGFITIVDVQRVLESINVQMDENTLHEILNEVDLNKNGQVELN
                     EFLQLMSAIQKGRVSGSRLAILMKTAEENLDRRVPIPVDRSCGGL"
     exon            246..417
                     /gene="GPD2"
                     /gene_synonym="GDH2; GPDM; mGDH; mGPDH"
                     /inference="alignment:Splign:2.1.0"
     exon            418..542
                     /gene="GPD2"
                     /gene_synonym="GDH2; GPDM; mGDH; mGPDH"
                     /inference="alignment:Splign:2.1.0"
     exon            543..640
                     /gene="GPD2"
                     /gene_synonym="GDH2; GPDM; mGDH; mGPDH"
                     /inference="alignment:Splign:2.1.0"
     exon            641..804
                     /gene="GPD2"
                     /gene_synonym="GDH2; GPDM; mGDH; mGPDH"
                     /inference="alignment:Splign:2.1.0"
     exon            805..969
                     /gene="GPD2"
                     /gene_synonym="GDH2; GPDM; mGDH; mGPDH"
                     /inference="alignment:Splign:2.1.0"
     exon            970..1114
                     /gene="GPD2"
                     /gene_synonym="GDH2; GPDM; mGDH; mGPDH"
                     /inference="alignment:Splign:2.1.0"
     exon            1115..1308
                     /gene="GPD2"
                     /gene_synonym="GDH2; GPDM; mGDH; mGPDH"
                     /inference="alignment:Splign:2.1.0"
     exon            1309..1443
                     /gene="GPD2"
                     /gene_synonym="GDH2; GPDM; mGDH; mGPDH"
                     /inference="alignment:Splign:2.1.0"
     exon            1444..1619
                     /gene="GPD2"
                     /gene_synonym="GDH2; GPDM; mGDH; mGPDH"
                     /inference="alignment:Splign:2.1.0"
     exon            1620..1751
                     /gene="GPD2"
                     /gene_synonym="GDH2; GPDM; mGDH; mGPDH"
                     /inference="alignment:Splign:2.1.0"
     exon            1752..1910
                     /gene="GPD2"
                     /gene_synonym="GDH2; GPDM; mGDH; mGPDH"
                     /inference="alignment:Splign:2.1.0"
     exon            1911..2023
                     /gene="GPD2"
                     /gene_synonym="GDH2; GPDM; mGDH; mGPDH"
                     /inference="alignment:Splign:2.1.0"
     exon            2024..2102
                     /gene="GPD2"
                     /gene_synonym="GDH2; GPDM; mGDH; mGPDH"
                     /inference="alignment:Splign:2.1.0"
     exon            2103..2201
                     /gene="GPD2"
                     /gene_synonym="GDH2; GPDM; mGDH; mGPDH"
                     /inference="alignment:Splign:2.1.0"
     exon            2202..5812
                     /gene="GPD2"
                     /gene_synonym="GDH2; GPDM; mGDH; mGPDH"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5542..5547
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GPD2"
                     /gene_synonym="GDH2; GPDM; mGDH; mGPDH"
                     /note="hexamer: AATAAA"
     polyA_site      5563
                     /gene="GPD2"
                     /gene_synonym="GDH2; GPDM; mGDH; mGPDH"
     regulatory      5788..5793
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GPD2"
                     /gene_synonym="GDH2; GPDM; mGDH; mGPDH"
                     /note="hexamer: ATTACA"
     polyA_site      5812
                     /gene="GPD2"
                     /gene_synonym="GDH2; GPDM; mGDH; mGPDH"
                     /note="major polyA site"
ORIGIN      
        1 gagtgacagc cccgcgcgcc tcgctgggag cacccgggcc gaggctctga ttctgggggg
       61 aggccgactc caccctggct ggaggaactg ggtgctcctg cccgctggcc cctcgcgcgt
      121 gaggatctat ctcaggctaa gaaatggcat ttcaaaaggc agtgaaaggg acgattcttg
      181 ttggaggagg tgctcttgca actgttttag gactttctca gtttgctcat tacagaagga
      241 aacaaatgaa cctggcctat gttaaagcag cagactgcat ttcagaacca gttaacaggg
      301 agcctccttc cagagaagct cagctactga ctttgcaaaa cacatctgaa tttgatatcc
      361 ttgttattgg aggaggagca acaggaagtg gctgtgcgct agatgctgtc accagaggac
      421 taaaaacagc ccttgtagaa agagatgatt tctcatcagg gaccagcagc agaagcacta
      481 aattgatcca tggtggtgtg agatatctgc agaaggccat catgaagttg gatattgagc
      541 agtataggat ggtaaaagaa gcccttcatg agcgtgccaa cctgctagaa attgctcccc
      601 atttatcagc tccattgcct ataatgcttc cagtttacaa gtggtggcag ttaccttact
      661 actgggtagg aatcaagctg tatgatttgg ttgcaggaag caattgccta aaaagcagtt
      721 atgtcctcag caaatcaaga gcccttgaac atttcccaat gctccagaag gacaaactgg
      781 taggagcaat tgtctactat gacggacaac ataacgatgc acggatgaac cttgccattg
      841 ctctgactgc tgccaggtat ggggctgcca cagccaatta catggaggta gtgagcttgc
      901 tcaagaagac agacccccag acagggaaag tgcgtgtgag cggcgcacgg tgcaaggatg
      961 tcctcacagg gcaggaattt gacgtgagag ccaaatgtgt tatcaatgcc acgggacctt
     1021 tcacggactc tgtgcgcaaa atggatgata aagacgcagc agctatctgc cagccaagtg
     1081 ctggtgtcca tattgtgatg cctggttatt acagcccaga gagcatggga cttcttgacc
     1141 cagcgaccag tgatgggcga gttattttct tcttaccctg gcaaaagatg acgatcgctg
     1201 gcactactga tactccaact gatgttacac accatccaat tccttcagaa gaagatatca
     1261 acttcatttt gaatgaagtg cgtaattacc tgagttgtga tgttgaagtg agaagagggg
     1321 atgtcctggc agcatggagt ggaatccgtc ctcttgttac agaccccaaa tctgcagata
     1381 ctcagtctat ctcccgaaat catgttgttg atatcagtga gagtggcctt attactatag
     1441 caggtggaaa gtggacaact tatcggtcta tggcagaaga taccataaat gctgctgtca
     1501 aaactcataa tttaaaagca ggaccaagta gaacagttgg gcttttcctt caagggggta
     1561 aagattggag ccccacactc tacattaggc ttgtgcagga ttatggactt gaaagcgagg
     1621 tggcacagca tcttgccgcc acctatggtg ataaggcctt tgaggtggcc aaaatggcaa
     1681 gtgtgactgg caaaaggtgg cctattgttg gagtacgtct tgtgtcagaa tttccatata
     1741 ttgaagcaga ggtgaaatat gggattaagg agtatgcctg cactgctgtg gatatgattt
     1801 cacgtcgtac tcgcctggcc tttctaaatg tccaggcagc agaggaagcc ctacccagga
     1861 ttgttgaact gatgggcagg gaactgaatt gggatgatta taagaagcag gaacaacttg
     1921 aaacagccag gaagtttcta tattatgaaa tgggctataa atctcgatca gaacagttaa
     1981 cagatcgctc tgaaattagc ctactgcctt cagacattga caggtataag aagagatttc
     2041 ataagtttga tgcagaccag aaaggcttta ttaccattgt tgatgttcag cgtgtattag
     2101 agagtatcaa tgtccaaatg gatgaaaata cactccatga aattctaaat gaagttgatt
     2161 tgaataaaaa tggacaggtt gaactcaatg aatttttgca gctgatgagt gctattcaaa
     2221 aaggaagggt atctggaagc cggcttgcta tactaatgaa aactgcagaa gagaacctcg
     2281 acagaagagt tccaattcca gtggaccgta gttgtggagg attgtgagtc tgggcagtaa
     2341 atccacagcc aacaaacata gaaacgacaa atcaccatgt aacaaccaga gatgactgaa
     2401 accactctga aataatgaat gtggatagct gcctttttta acactagaaa acattccaaa
     2461 actttaaggt gttggtgtat ttgccagctt tatttgctgt actttatttg tatttgccat
     2521 tcagtctagc ttttaagtat atttttttct ttttctcatt ttcaatgcac attagttttg
     2581 catctgtttt gtgacctgtt agatgtgaca cattctcttt ttgtttattc ccttattcta
     2641 aatgagttct aaaaacataa tattttggca aaaattgaaa aaagctggag acattttgtg
     2701 acatgcatac agatagcatg tgttattaaa aagagttgcc tattgaaatg atcatgtttc
     2761 tggagaaatt aagcttatag acagcatgtg ttattaaaaa gagttaccta ttgaaatgat
     2821 agtgtttctg gagatttctc agccccatct tcctccagct tgtctacctt cctcatgcaa
     2881 acaatattcc acatttttat atctagagca tgactggcta actggagagg gaaggaaaag
     2941 tttagatctg gttactggag caagtctcaa agagaaactc tgaaagcttc cagaatcaca
     3001 ggtataagat aaggatagca ttgacatttg ctgggagtta cagtgatagt ttcatctcag
     3061 cagttcattt ttttcttcag tcactgctgg ttttctttga ctattatagt tgccaggaag
     3121 atccttgctc ttcttacttt aaaaccagca tttaagtggc aatttggatg taataggatg
     3181 agaccaaatt tatctaatta ttacagtagt aatacattta agagttaaaa atgtgttttt
     3241 atatatccac atatgataac aaaagattca attcccaagc ctaaattttg aagcatgtgg
     3301 tattgtcaaa tctagcttac catctttttt gggggtactt gcaaccatag taaaggaaga
     3361 tggaatagac tttagttaac tttaattata attatatgtt aaataaggca cataaccagt
     3421 ttccaaggtc atcatggttg cttaaagtct ttccccttct gtactccatg gaaatattct
     3481 cagtaaacca aaaacaaaaa tggaaaaata atcaccaacc cccatcccga cacacacaca
     3541 gagtccaaag caaaagtcag tgtgtattga atttaacaag taatgcagtt tgggatgctt
     3601 ttgctacatt ttggtggcat tttaactagt tatctgaata tttattaatc gtacttcctc
     3661 ttgtaaagtt aactacttac ttttttgttg ttgttttttt aacatcaggt tctgtatcta
     3721 ataggagatg taacacttta tttcatggca ggtttttatt gcagagactt gaagtcttag
     3781 ttttttaaac tggcacataa aacacttttt gctgttattt ttatttatgt caatactgca
     3841 gagtatcttt atgccttatt caagtggatt ctgagcctgt atgtcacaat gtaaacactg
     3901 gaggttcact cacctacgca ctcacccacc acctctgaaa gaaacagaaa ctgcagagaa
     3961 agacagcatc ttagctcatt ttgtttttaa atgaggtttt agacgcttgc cacttcctaa
     4021 gggaaatcct aaaacagagc aagtgatgct cccaggtatc actgtgaact tttttctttc
     4081 aaagtgtgaa tttttacact ggctttttca tttttttaaa gtaattgaag cttgtggctt
     4141 tacaacttag tgttttttgc tatccagata acaagtttca ttgtttagaa cccagtgaca
     4201 cttaataggt agataaattg tcctttaaaa tatcccagat gatatacaca atatggtaca
     4261 tttgtgctct ctctctctgt ttttctctct ttctctttct agttagatca agataacgat
     4321 gacttgtacc ctccctgatt ctgttacagt agggcccggg cagatctgtg tttgttaaac
     4381 aggccttgtt tgtgcatgct ttgctatgaa tgaagttcct ttaaggacaa agaaaagcac
     4441 acttttcttc ttttgagcat atctgctttt actttaaatc tgctaatttc taaaatgtag
     4501 agtccttcac caatcccaga gactcaattt ggaaatgaac tgatttcaag ccgttactgt
     4561 gaataaagca ccccagcatt ttgtataagc tcttaattaa acctgtacag cttctttacc
     4621 tgatttaaga aagggagaca agcaataggg gggaataagc ttcttcaaaa ttcattccaa
     4681 gcacaaaaga aattttttcc ctttgcatat aaacttgaca tcagttgatt ctgttggggt
     4741 cggggtgggt atgcagggat tgccttttat tatcaagtga tttatttcaa gagcctttga
     4801 ggggattcag gtgaaggaac ccccatctgt gctggagagt tggatactcc tttaaatagc
     4861 ccccaccaca gtcataacaa taatgataat gctggattat ttgttaagcc aaggttgtct
     4921 gcctcatatc catcatgctg tttgcaatat tcttgctttc aatcaattta tactgagtgt
     4981 aactttagga tttctgctat taaatgcatg ctctctatcc tgcttcagtt tctggctttg
     5041 ctttgtctgt ttcaaaatat gtagcttcct cttttgtaca acaaaaaact cattctcact
     5101 tttactaaat atactgtagg agtcatcatt gatgttattt ttctcttatg tatctgtaaa
     5161 gatttttggc atatgaatgt aatattaaag tcaatgatgc tataacttgc gatgtttgca
     5221 tcatgtcaac ctttttgaag gagtgaaaaa gccctactat gtttttaaat agcaagtgta
     5281 agctcagtgc tagagtggat atacacaccg catgttttca tatgtggcac ttttatgtat
     5341 catgttgggt tattgttcta gactggactg ttaaatacta tgtttgaggc tgggttgtca
     5401 tttttataac tgtcttggtg ttttatggcc attatttatt acttttgata cacagaatga
     5461 gctgcatgca tttatagagc aataagagga tgtatttaat gtgccttgtt tttaactgaa
     5521 taagaactgg aagcatgaat caataaaact gattaaaatg gtctatttgc tagcattttg
     5581 atgttacttg cagtcagata actttgatta ctgttgaagt ttaaaaaaag tttgaaaata
     5641 tttttacaaa ctgtgttttt gatgacacaa aagtgaaata tctacagaga tagatgtaat
     5701 tttataagac tgccagaatt atttgtatta atttgttgct gtagccttta gggcatgact
     5761 tctgtatttg tgcaatccta ttctacaatt acattcatcc tattacaact ca
//
