LOCUS       NM_004466               2943 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens glypican 5 (GPC5), mRNA.
ACCESSION   NM_004466
VERSION     NM_004466.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2943)
  AUTHORS   Yang,X., Chen,Y., Zhou,Y., Wu,C., Li,Q., Wu,J., Hu,W.W., Zhao,W.Q.,
            Wei,W., Wu,C.P., Jiang,J.T. and Ji,M.
  TITLE     GPC5 suppresses lung cancer progression and metastasis via
            intracellular CTDSP1/AhR/ARNT signaling axis and extracellular
            exosome secretion
  JOURNAL   Oncogene 40 (25), 4307-4323 (2021)
   PUBMED   34079082
  REMARK    GeneRIF: GPC5 suppresses lung cancer progression and metastasis via
            intracellular CTDSP1/AhR/ARNT signaling axis and extracellular
            exosome secretion.
REFERENCE   2  (bases 1 to 2943)
  AUTHORS   Schlaepfer Sales,C.B., Guimaraes,V.S.N., Valverde,L.F., Dias,R.B.,
            Freitas,R.D., Siquara da Rocha,L.O., Coelho de Miranda,M., Vilas
            Boas,D.S., Goncalves Agra,I.M., Dos Santos,J.N. and Gurgel
            Rocha,C.A.
  TITLE     Glypican-1, -3, -5 (GPC1, GPC3, GPC5) and Hedgehog Pathway
            Expression in Oral Squamous Cell Carcinoma
  JOURNAL   Appl Immunohistochem Mol Morphol 29 (5), 345-351 (2021)
   PUBMED   33512817
  REMARK    GeneRIF: Glypican-1, -3, -5 (GPC1, GPC3, GPC5) and Hedgehog Pathway
            Expression in Oral Squamous Cell Carcinoma.
REFERENCE   3  (bases 1 to 2943)
  AUTHORS   Takeuchi,M., Takeuchi,K., Takai,T., Yamaguchi,R., Furukawa,T.,
            Akagi,K.I. and Takeuchi,J.K.
  TITLE     Subcellular localization of glypican-5 is associated with dynamic
            motility of the human mesenchymal stem cell line U3DT
  JOURNAL   PLoS One 16 (2), e0226538 (2021)
   PUBMED   33606708
  REMARK    GeneRIF: Subcellular localization of glypican-5 is associated with
            dynamic motility of the human mesenchymal stem cell line U3DT.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2943)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   5  (bases 1 to 2943)
  AUTHORS   Hong,X., Zhang,Z., Pan,L., Ma,W., Zhai,X., Gu,C., Zhang,Y., Bi,X.,
            Huang,W., Pei,H. and Liu,Z.
  TITLE     MicroRNA-301b promotes the proliferation and invasion of glioma
            cells through enhancing activation of Wnt/beta-catenin signaling
            via targeting Glypican-5
  JOURNAL   Eur J Pharmacol 854, 39-47 (2019)
   PUBMED   30951720
  REMARK    GeneRIF: miR-301b represses the proliferation and invasion of
            glioma cells by up-regulating GPC5 expression
REFERENCE   6  (bases 1 to 2943)
  AUTHORS   Veugelers,M., De Cat,B., Delande,N., Esselens,C., Bonk,I.,
            Vermeesch,J., Marynen,P., Fryns,J.P. and David,G.
  TITLE     A 4-Mb BAC/PAC contig and complete genomic structure of the
            GPC5/GPC6 gene cluster on chromosome 13q32
  JOURNAL   Matrix Biol 20 (5-6), 375-385 (2001)
   PUBMED   11566272
REFERENCE   7  (bases 1 to 2943)
  AUTHORS   De Cat,B. and David,G.
  TITLE     Developmental roles of the glypicans
  JOURNAL   Semin Cell Dev Biol 12 (2), 117-125 (2001)
   PUBMED   11292377
  REMARK    Review article
REFERENCE   8  (bases 1 to 2943)
  AUTHORS   Saunders,S., Paine-Saunders,S. and Lander,A.D.
  TITLE     Expression of the cell surface proteoglycan glypican-5 is
            developmentally regulated in kidney, limb, and brain
  JOURNAL   Dev Biol 190 (1), 78-93 (1997)
   PUBMED   9331333
REFERENCE   9  (bases 1 to 2943)
  AUTHORS   Veugelers,M., Vermeesch,J., Reekmans,G., Steinfeld,R., Marynen,P.
            and David,G.
  TITLE     Characterization of glypican-5 and chromosomal localization of
            human GPC5, a new member of the glypican gene family
  JOURNAL   Genomics 40 (1), 24-30 (1997)
   PUBMED   9070915
REFERENCE   10 (bases 1 to 2943)
  AUTHORS   Watanabe,K., Yamada,H. and Yamaguchi,Y.
  TITLE     K-glypican: a novel GPI-anchored heparan sulfate proteoglycan that
            is highly expressed in developing brain and kidney
  JOURNAL   J Cell Biol 130 (5), 1207-1218 (1995)
   PUBMED   7657705
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DB100549.1, HY017486.1 and
            BC039730.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_004466.5.
            
            Summary: Cell surface heparan sulfate proteoglycans are composed of
            a membrane-associated protein core substituted with a variable
            number of heparan sulfate chains. Members of the glypican-related
            integral membrane proteoglycan family (GRIPS) contain a core
            protein anchored to the cytoplasmic membrane via a glycosyl
            phosphatidylinositol linkage.  These proteins may play a role in
            the control of cell division and growth regulation. [provided by
            RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC039730.1, SRR1660809.180115.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968968, SAMEA2145313
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000377067.9/ ENSP00000366267.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-38                DB100549.1         3-40
            39-62               HY017486.1         23-46
            63-2943             BC039730.1         27-2907
FEATURES             Location/Qualifiers
     source          1..2943
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="13"
                     /map="13q31.3"
     gene            1..2943
                     /gene="GPC5"
                     /note="glypican 5"
                     /db_xref="GeneID:2262"
                     /db_xref="HGNC:HGNC:4453"
                     /db_xref="MIM:602446"
     exon            1..589
                     /gene="GPC5"
                     /inference="alignment:Splign:2.1.0"
     CDS             427..2145
                     /gene="GPC5"
                     /note="glypican proteoglycan 5; bA93M14.1"
                     /codon_start=1
                     /product="glypican-5 precursor"
                     /protein_id="NP_004457.1"
                     /db_xref="CCDS:CCDS9468.1"
                     /db_xref="GeneID:2262"
                     /db_xref="HGNC:HGNC:4453"
                     /db_xref="MIM:602446"
                     /translation="MDAQTWPVGFRCLLLLALVGSARSEGVQTCEEVRKLFQWRLLGA
                     VRGLPDSPRAGPDLQVCISKKPTCCTRKMEERYQIAARQDMQQFLQTSSSTLKFLISR
                     NAAAFQETLETLIKQAENYTSILFCSTYRNMALEAAASVQEFFTDVGLYLFGADVNPE
                     EFVNRFFDSLFPLVYNHLINPGVTDSSLEYSECIRMARRDVSPFGNIPQRVMGQMGRS
                     LLPSRTFLQALNLGIEVINTTDYLHFSKECSRALLKMQYCPHCQGLALTKPCMGYCLN
                     VMRGCLAHMAELNPHWHAYIRSLEELSDAMHGTYDIGHVLLNFHLLVNDAVLQAHLNG
                     QKLLEQVNRICGRPVRTPTQSPRCSFDQSKEKHGMKTTTRNSEETLANRRKEFINSLR
                     LYRSFYGGLADQLCANELAAADGLPCWNGEDIVKSYTQRVVGNGIKAQSGNPEVKVKG
                     IDPVINQIIDKLKHVVQLLQGRSPKPDKWELLQLGSGGGMVEQVSGDCDDEDGCGGSG
                     SGEVKRTLKITDWMPDDMNFSDVKQIHQTDTGSTLDTTGAGCAVATESMTFTLISVVM
                     LLPGIW"
     exon            590..751
                     /gene="GPC5"
                     /inference="alignment:Splign:2.1.0"
     exon            752..1446
                     /gene="GPC5"
                     /inference="alignment:Splign:2.1.0"
     exon            1447..1580
                     /gene="GPC5"
                     /inference="alignment:Splign:2.1.0"
     exon            1581..1706
                     /gene="GPC5"
                     /inference="alignment:Splign:2.1.0"
     exon            1707..1827
                     /gene="GPC5"
                     /inference="alignment:Splign:2.1.0"
     exon            1828..1987
                     /gene="GPC5"
                     /inference="alignment:Splign:2.1.0"
     exon            1988..2943
                     /gene="GPC5"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2914..2919
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GPC5"
                     /note="hexamer: AATAAA"
     polyA_site      2943
                     /gene="GPC5"
                     /note="major polyA site"
ORIGIN      
        1 atcccaggtt agctgctgcg agccgagccg ggcggcggag gcggcggcgg cggcggcagt
       61 ggcggcagtg gcggcagtgg cggcagcggc agcagttgca gcagtggtgg ccagagcgga
      121 tgcttgcggg ctccctgcgg ctccactagt tttcttcgcc ccgcccagcc gcccactctt
      181 ctcggctagg gaagaagacc agagggtgct cagctggaaa actctggtgt ctcagcttag
      241 ggcctcctcc gggaagagct aactgctccc aggtgaagcc ggtgcccgcg ggcggtccgt
      301 acaccccgca gccggctcgc accgctcgag agcctcggcc gctgtgtctt ccacgtctgc
      361 agctcagcca gggcgcgcag ggcgagtggg gtccactggc gggtaaaggg gaccaggacg
      421 gcgaggatgg acgcacagac ctggcccgtg ggctttcgct gcctcctcct tctggccctg
      481 gttgggtccg cccgcagcga gggcgtgcag acctgcgaag aagttcggaa acttttccag
      541 tggcggctgc tgggagctgt cagggggctg ccggattcgc cgcgggcagg acctgatctt
      601 caggtttgca tatccaaaaa gcctacatgt tgcaccagga agatggagga gagatatcag
      661 attgcggctc gccaggatat gcagcagttt cttcaaacgt ccagctctac attaaagttt
      721 ctaatatctc gaaatgcggc tgcttttcaa gaaacccttg aaactctcat caaacaagca
      781 gaaaattaca ccagtatact tttttgcagt acctacagga acatggcctt ggaggctgct
      841 gcttcggttc aggagttctt cactgatgtg gggctgtatt tatttggtgc ggatgttaat
      901 cctgaagaat ttgtaaacag attttttgac agtctttttc ctctggtcta caaccacctc
      961 attaaccctg gtgtgactga cagttccctg gaatactcag aatgcatccg gatggctcgc
     1021 cgggatgtga gtccatttgg taatattccc caaagagtaa tgggacagat ggggaggtcc
     1081 ctgctgccca gccgcacttt tctgcaggca ctcaatctgg gcattgaagt catcaacacc
     1141 acagactatc tgcacttctc caaagagtgc agcagagccc tcctgaagat gcaatactgc
     1201 ccgcactgcc aaggcctggc gctcactaag ccttgtatgg gatactgcct caatgtcatg
     1261 cgaggctgcc tggcgcacat ggcggagctt aatccacact ggcatgcata tatccggtcg
     1321 ttggaagaac tctcggatgc aatgcatgga acatacgaca ttggacacgt gctgctgaac
     1381 tttcacttgc ttgttaatga tgctgtgtta caggctcacc tcaatggaca aaaattattg
     1441 gaacaggtaa ataggatttg tggccgccct gtaagaacac ccacacaaag cccccgttgt
     1501 tcttttgatc agagcaaaga gaagcatgga atgaagacca ccacaaggaa cagtgaagag
     1561 acgcttgcca acagaagaaa agaatttatc aacagccttc gactgtacag gtcattctat
     1621 ggaggtctag ctgatcagct ttgtgctaat gaattagctg ctgcagatgg acttccctgc
     1681 tggaatggag aagatatagt aaaaagttat actcagcgtg tggttggaaa tggaatcaaa
     1741 gcccagtctg gaaatcctga agtcaaagtc aaaggaattg atcctgtgat aaatcagatt
     1801 attgataaac tgaagcatgt tgttcagttg ttacagggta gatcacccaa acctgacaag
     1861 tgggaacttc ttcagctggg cagtggtgga ggcatggttg aacaagtcag tggggactgt
     1921 gatgatgaag atggttgcgg gggatcagga agtggagaag tcaagaggac actgaagatc
     1981 acagactgga tgccagatga tatgaacttc agtgatgtaa agcaaatcca tcaaacagac
     2041 actggcagta ctttagacac aacaggagca ggatgtgcag tggcgactga atctatgaca
     2101 ttcactctga taagtgtggt gatgttactt cccgggattt ggtaactgaa ctcttctgtc
     2161 ctgacatacc ttactgaagt ctcgatttct tctctctctg catatgcctg gaataagaga
     2221 tcctttttca atgtaacaat tatatttatg aaaagatatg ttacactaac ttctcagaag
     2281 ccaagctgaa atattcataa agtccctaaa actcaacgtt taaatgacac actttaaaaa
     2341 tatgtctttt ttcaatctaa ctgaaaacct tcttaacttc taatatatta aatctgaaga
     2401 tgtgaagggc acagaagtga ctttgaataa gaagaattta gtgtatctgt aattttatta
     2461 tcaattccaa gccccttcct ttctaaatta aaaatgtttt catttgaaag tgtatttgcc
     2521 agacaatgaa aacagtatgc agtatttctt aaagtattga aattagaata tcatgaaata
     2581 aatcaaaaca tacaatggca agtagtatgc atgcatattc aagagactct tccatttttg
     2641 caagctgtag aaggaaatgt ctgaatgtct ataagttatg gggtagattc ttgagaagca
     2701 tttcatataa tttcactgaa gaaccttgat aattttgacc cactgtaact tagccactga
     2761 tgaaccttaa agctgagtat tttattaaca cctgatttgt attccattat attcaaaatg
     2821 catctttggt attgtgcctc tgctcccatc tctctctttg cctcatagat ttagctatgt
     2881 tgggaagcac atgcttgctc taggaatatc tccaataaag ctgttaacta tttggtggaa
     2941 aaa
//
