LOCUS       NM_178819               6298 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens glycerol-3-phosphate acyltransferase 4 (GPAT4),
            transcript variant 1, mRNA.
ACCESSION   NM_178819
VERSION     NM_178819.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6298)
  AUTHORS   Chang,C.L., Weigel,A.V., Ioannou,M.S., Pasolli,H.A., Xu,C.S.,
            Peale,D.R., Shtengel,G., Freeman,M., Hess,H.F., Blackstone,C. and
            Lippincott-Schwartz,J.
  TITLE     Spastin tethers lipid droplets to peroxisomes and directs fatty
            acid trafficking through ESCRT-III
  JOURNAL   J Cell Biol 218 (8), 2583-2599 (2019)
   PUBMED   31227594
REFERENCE   2  (bases 1 to 6298)
  AUTHORS   Zhu,X.G., Nicholson Puthenveedu,S., Shen,Y., La,K., Ozlu,C.,
            Wang,T., Klompstra,D., Gultekin,Y., Chi,J., Fidelin,J., Peng,T.,
            Molina,H., Hang,H.C., Min,W. and Birsoy,K.
  TITLE     CHP1 Regulates Compartmentalized Glycerolipid Synthesis by
            Activating GPAT4
  JOURNAL   Mol Cell 74 (1), 45-58 (2019)
   PUBMED   30846317
  REMARK    GeneRIF: GPAT4 activity requires CHP1 to be N-myristoylated,
            forming a key molecular interface between the two proteins,
            regulating glycerolipid metabolism.
REFERENCE   3  (bases 1 to 6298)
  AUTHORS   Cooper,D.E., Grevengoed,T.J., Klett,E.L. and Coleman,R.A.
  TITLE     Glycerol-3-phosphate Acyltransferase Isoform-4 (GPAT4) Limits
            Oxidation of Exogenous Fatty Acids in Brown Adipocytes
  JOURNAL   J Biol Chem 290 (24), 15112-15120 (2015)
   PUBMED   25918168
REFERENCE   4  (bases 1 to 6298)
  AUTHORS   Snogdal,L.S., Grarup,N., Banasik,K., Wod,M., Jorgensen,T.,
            Witte,D.R., Lauritzen,T., Nielsen,A.A., Brandslund,I.,
            Christensen,C., Pedersen,O., Yderstraede,K., Beck-Nielsen,H.,
            Henriksen,J.E., Hansen,T. and Hojlund,K.
  TITLE     Studies of association of AGPAT6 variants with type 2 diabetes and
            related metabolic phenotypes in 12,068 Danes
  JOURNAL   BMC Med Genet 14, 113 (2013)
   PUBMED   24156295
  REMARK    GeneRIF: None of the eleven AGPAT6 variants were robustly
            associated with type 2 diabetes in the Danish.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 6298)
  AUTHORS   Shan,D., Li,J.L., Wu,L., Li,D., Hurov,J., Tobin,J.F., Gimeno,R.E.
            and Cao,J.
  TITLE     GPAT3 and GPAT4 are regulated by insulin-stimulated phosphorylation
            and play distinct roles in adipogenesis
  JOURNAL   J Lipid Res 51 (7), 1971-1981 (2010)
   PUBMED   20181984
  REMARK    GeneRIF: Results reveal a link between the lipogenic effects of
            insulin and microsomal GPAT3 and GPAT4, implying their importance
            in glycerolipid biosynthesis.
REFERENCE   6  (bases 1 to 6298)
  AUTHORS   Ghosh,D., Lippert,D., Krokhin,O., Cortens,J.P. and Wilkins,J.A.
  TITLE     Defining the membrane proteome of NK cells
  JOURNAL   J Mass Spectrom 45 (1), 1-25 (2010)
   PUBMED   19946888
REFERENCE   7  (bases 1 to 6298)
  AUTHORS   Chen,Y.Q., Kuo,M.S., Li,S., Bui,H.H., Peake,D.A., Sanders,P.E.,
            Thibodeaux,S.J., Chu,S., Qian,Y.W., Zhao,Y., Bredt,D.S.,
            Moller,D.E., Konrad,R.J., Beigneux,A.P., Young,S.G. and Cao,G.
  TITLE     AGPAT6 is a novel microsomal glycerol-3-phosphate acyltransferase
  JOURNAL   J Biol Chem 283 (15), 10048-10057 (2008)
   PUBMED   18238778
  REMARK    GeneRIF: AGPAT6 is a microsomal GPAT, and we propose renaming this
            enzyme GPAT4.
REFERENCE   8  (bases 1 to 6298)
  AUTHORS   Tan,X.J., Huang,Z.P., Li,L.Y., Nie,D.S., Zhong,C.G., Fu,J.J. and
            Lu,G.X.
  TITLE     Molecular cloning and preliminary function study of a novel human
            gene, TSARG7, related to spermatogenesis
  JOURNAL   Yi Chuan Xue Bao 33 (4), 294-303 (2006)
   PUBMED   16625827
  REMARK    GeneRIF: we have cloned a novel human gene and this gene may play
            an important role in human spermatogenesis and sexual maturation.
REFERENCE   9  (bases 1 to 6298)
  AUTHORS   Li,D., Yu,L., Wu,H., Shan,Y., Guo,J., Dang,Y., Wei,Y. and Zhao,S.
  TITLE     Cloning and identification of the human LPAAT-zeta gene, a novel
            member of the lysophosphatidic acid acyltransferase family
  JOURNAL   J Hum Genet 48 (8), 438-442 (2003)
   PUBMED   12938015
REFERENCE   10 (bases 1 to 6298)
  AUTHORS   Lehner,R. and Kuksis,A.
  TITLE     Biosynthesis of triacylglycerols
  JOURNAL   Prog Lipid Res 35 (2), 169-201 (1996)
   PUBMED   8944226
  REMARK    Review article
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AF406612.1, AC009630.14 and BU620295.1.
            
            On Nov 23, 2018 this sequence version replaced NM_178819.3.
            
            Summary: Lysophosphatidic acid acyltransferases (EC 2.3.1.51)
            catalyze the conversion of lysophosphatidic acid (LPA) to
            phosphatidic acid (PA). LPA and PA are involved in signal
            transduction and lipid biosynthesis.[supplied by OMIM, Apr 2004].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803611.232280.1,
                                           SRR1660807.184189.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000396987.7/ ENSP00000380184.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2592              AF406612.1         1-2592
            2593-5953           AC009630.14        23969-27329         c
            5954-6298           BU620295.1         19-363              c
FEATURES             Location/Qualifiers
     source          1..6298
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="8"
                     /map="8p11.21"
     gene            1..6298
                     /gene="GPAT4"
                     /gene_synonym="1-AGPAT 6; AGPAT6; LPAAT-zeta; LPAATZ;
                     TSARG7"
                     /note="glycerol-3-phosphate acyltransferase 4"
                     /db_xref="GeneID:137964"
                     /db_xref="HGNC:HGNC:20880"
                     /db_xref="MIM:608143"
     exon            1..79
                     /gene="GPAT4"
                     /gene_synonym="1-AGPAT 6; AGPAT6; LPAAT-zeta; LPAATZ;
                     TSARG7"
                     /inference="alignment:Splign:2.1.0"
     exon            80..1092
                     /gene="GPAT4"
                     /gene_synonym="1-AGPAT 6; AGPAT6; LPAAT-zeta; LPAATZ;
                     TSARG7"
                     /inference="alignment:Splign:2.1.0"
     CDS             928..2298
                     /gene="GPAT4"
                     /gene_synonym="1-AGPAT 6; AGPAT6; LPAAT-zeta; LPAATZ;
                     TSARG7"
                     /EC_number="2.3.1.15"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; 1-acyl-sn-glycerol-3-phosphate acyltransferase
                     zeta; 1-AGP acyltransferase 6; glycerol-3-phosphate
                     acyltransferase 6; testis spermatogenesis
                     apoptosis-related protein 7; lysophosphatidic acid
                     acyltransferase zeta; 1-acylglycerol-3-phosphate
                     O-acyltransferase 6 (lysophosphatidic acid
                     acyltransferase, zeta)"
                     /codon_start=1
                     /product="glycerol-3-phosphate acyltransferase 4 isoform 1
                     precursor"
                     /protein_id="NP_848934.1"
                     /db_xref="CCDS:CCDS6117.1"
                     /db_xref="GeneID:137964"
                     /db_xref="HGNC:HGNC:20880"
                     /db_xref="MIM:608143"
                     /translation="MFLLLPFDSLIVNLLGISLTVLFTLLLVFIIVPAIFGVSFGIRK
                     LYMKSLLKIFAWATLRMERGAKEKNHQLYKPYTNGIIAKDPTSLEEEIKEIRRSGSSK
                     ALDNTPEFELSDIFYFCRKGMETIMDDEVTKRFSAEELESWNLLSRTNYNFQYISLRL
                     TVLWGLGVLIRYCFLLPLRIALAFTGISLLVVGTTVVGYLPNGRFKEFMSKHVHLMCY
                     RICVRALTAIITYHDRENRPRNGGICVANHTSPIDVIILASDGYYAMVGQVHGGLMGV
                     IQRAMVKACPHVWFERSEVKDRHLVAKRLTEHVQDKSKLPILIFPEGTCINNTSVMMF
                     KKGSFEIGATVYPVAIKYDPQFGDAFWNSSKYGMVTYLLRMMTSWAIVCSVWYLPPMT
                     READEDAVQFANRVKSAIARQGGLVDLLWDGGLKREKVKDTFKEEQQKLYSKMIVGNH
                     KDRSRS"
     exon            1093..1162
                     /gene="GPAT4"
                     /gene_synonym="1-AGPAT 6; AGPAT6; LPAAT-zeta; LPAATZ;
                     TSARG7"
                     /inference="alignment:Splign:2.1.0"
     exon            1163..1463
                     /gene="GPAT4"
                     /gene_synonym="1-AGPAT 6; AGPAT6; LPAAT-zeta; LPAATZ;
                     TSARG7"
                     /inference="alignment:Splign:2.1.0"
     exon            1464..1538
                     /gene="GPAT4"
                     /gene_synonym="1-AGPAT 6; AGPAT6; LPAAT-zeta; LPAATZ;
                     TSARG7"
                     /inference="alignment:Splign:2.1.0"
     exon            1539..1628
                     /gene="GPAT4"
                     /gene_synonym="1-AGPAT 6; AGPAT6; LPAAT-zeta; LPAATZ;
                     TSARG7"
                     /inference="alignment:Splign:2.1.0"
     exon            1629..1722
                     /gene="GPAT4"
                     /gene_synonym="1-AGPAT 6; AGPAT6; LPAAT-zeta; LPAATZ;
                     TSARG7"
                     /inference="alignment:Splign:2.1.0"
     exon            1723..1838
                     /gene="GPAT4"
                     /gene_synonym="1-AGPAT 6; AGPAT6; LPAAT-zeta; LPAATZ;
                     TSARG7"
                     /inference="alignment:Splign:2.1.0"
     exon            1839..1894
                     /gene="GPAT4"
                     /gene_synonym="1-AGPAT 6; AGPAT6; LPAAT-zeta; LPAATZ;
                     TSARG7"
                     /inference="alignment:Splign:2.1.0"
     exon            1895..1980
                     /gene="GPAT4"
                     /gene_synonym="1-AGPAT 6; AGPAT6; LPAAT-zeta; LPAATZ;
                     TSARG7"
                     /inference="alignment:Splign:2.1.0"
     exon            1981..2109
                     /gene="GPAT4"
                     /gene_synonym="1-AGPAT 6; AGPAT6; LPAAT-zeta; LPAATZ;
                     TSARG7"
                     /inference="alignment:Splign:2.1.0"
     exon            2110..2189
                     /gene="GPAT4"
                     /gene_synonym="1-AGPAT 6; AGPAT6; LPAAT-zeta; LPAATZ;
                     TSARG7"
                     /inference="alignment:Splign:2.1.0"
     exon            2190..6298
                     /gene="GPAT4"
                     /gene_synonym="1-AGPAT 6; AGPAT6; LPAAT-zeta; LPAATZ;
                     TSARG7"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3189..3194
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GPAT4"
                     /gene_synonym="1-AGPAT 6; AGPAT6; LPAAT-zeta; LPAATZ;
                     TSARG7"
                     /note="hexamer: AATAAA"
     polyA_site      3211
                     /gene="GPAT4"
                     /gene_synonym="1-AGPAT 6; AGPAT6; LPAAT-zeta; LPAATZ;
                     TSARG7"
                     /note="major polyA site"
     regulatory      4164..4169
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GPAT4"
                     /gene_synonym="1-AGPAT 6; AGPAT6; LPAAT-zeta; LPAATZ;
                     TSARG7"
                     /note="hexamer: AATAAA"
     polyA_site      4188
                     /gene="GPAT4"
                     /gene_synonym="1-AGPAT 6; AGPAT6; LPAAT-zeta; LPAATZ;
                     TSARG7"
     regulatory      6277..6282
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GPAT4"
                     /gene_synonym="1-AGPAT 6; AGPAT6; LPAAT-zeta; LPAATZ;
                     TSARG7"
                     /note="hexamer: AATAAA"
     polyA_site      6298
                     /gene="GPAT4"
                     /gene_synonym="1-AGPAT 6; AGPAT6; LPAAT-zeta; LPAATZ;
                     TSARG7"
ORIGIN      
        1 gcagcttggg cccgcggcgg cggcaagggc gggagggagc ggtcgccgcg ggatttggag
       61 ctgcctagcc tcgcggtcgc tttggcagca tgtaagcagc tgtttgccaa gaacccaggt
      121 cactgctaag aaagggtgcc ttcgggagaa gagtgtccag aggataccaa tgccagatgc
      181 atctggagtt acactcagca ctcgcagtat gagacattgt gtgccagcat ctctttcctt
      241 ctggcaaaga ctgtagctct ccaggtagga ggatcctgga agctgtgagc accaggagcc
      301 ttgccagagg aggatggggc cagatatgaa ctctctacca tgaacatggt tctcggctta
      361 tgaaggaatt ttaagtaaaa cagttattta atttccacat attcaagtca aaagccttct
      421 gtgtgaagtg ccagtgatta cccctccaca ggagttatca ggatttttct ggcaccaagt
      481 ttaattcttc ttcgtacttc tggtagtgac agatctgcag gacagattta tctgttgaat
      541 gctcttgggc aggaaaacca tgtaaaacct ctggaagcag catcaggaca gcagagcaga
      601 gcccccgtcc tcactgctca cttgcacaga aactccatct ggactcggat gcttttactg
      661 aagacccatc tagcttcaat catctttaga gtccatccat tctggagaga cctggcgttt
      721 gcagttgcct cctgtggccg tgtttttctg tcattctgtt cccaggcctt ctattcaggc
      781 ggttgaaggg tgtggacttt ggaatggggt ttgctgttct tcgggaactt gcttcctttc
      841 cctggctggt gctgtcagga aggaccatct gaaggctgca atttgttctt agggaggcag
      901 gtgctggcct ggcctggatc ttccaccatg ttcctgttgc tgccttttga tagcctgatt
      961 gtcaaccttc tgggcatctc cctgactgtc ctcttcaccc tccttctcgt tttcatcata
     1021 gtgccagcca tttttggagt ctcctttggt atccgcaaac tctacatgaa aagtctgtta
     1081 aaaatctttg cgtgggctac cttgagaatg gagcgaggag ccaaggagaa gaaccaccag
     1141 ctttacaagc cctacaccaa cggaatcatt gcaaaggatc ccacttcact agaagaagag
     1201 atcaaagaga ttcgtcgaag tggtagtagt aaggctctgg acaacactcc agagttcgag
     1261 ctctctgaca ttttctactt ttgccggaaa ggaatggaga ccattatgga tgatgaggtg
     1321 acaaagagat tctcagcaga agaactggag tcctggaacc tgctgagcag aaccaattat
     1381 aacttccagt acatcagcct tcggctcacg gtcctgtggg ggttaggagt gctgattcgg
     1441 tactgctttc tgctgccgct caggatagca ctggctttca cagggattag ccttctggtg
     1501 gtgggcacaa ctgtggtggg atacttgcca aatgggaggt ttaaggagtt catgagtaaa
     1561 catgttcact taatgtgtta ccggatctgc gtgcgagcgc tgacagccat catcacctac
     1621 catgacaggg aaaacagacc aagaaatggt ggcatctgtg tggccaatca tacctcaccg
     1681 atcgatgtga tcatcttggc cagcgatggc tattatgcca tggtgggtca agtgcacggg
     1741 ggactcatgg gtgtgattca gagagccatg gtgaaggcct gcccacacgt ctggtttgag
     1801 cgctcggaag tgaaggatcg ccacctggtg gctaagagac tgactgaaca tgtgcaagat
     1861 aaaagcaagc tgcctatcct catcttccca gaaggaacct gcatcaataa tacatcggtg
     1921 atgatgttca aaaagggaag ttttgaaatt ggagccacag tttaccctgt tgctatcaag
     1981 tatgaccctc aatttggcga tgccttctgg aacagcagca aatacgggat ggtgacgtac
     2041 ctgctgcgaa tgatgaccag ctgggccatt gtctgcagcg tgtggtacct gcctcccatg
     2101 actagagagg cagatgaaga tgctgtccag tttgcgaata gggtgaaatc tgccattgcc
     2161 aggcagggag gacttgtgga cctgctgtgg gatgggggcc tgaagaggga gaaggtgaag
     2221 gacacgttca aggaggagca gcagaagctg tacagcaaga tgatcgtggg gaaccacaag
     2281 gacaggagcc gctcctgagc ctgcctccag ctggctgggg ccaccgtgcg gggtgccaac
     2341 gggctcagag ctggagttgc cgccgccgcc cccactgctg tgtcctttcc agactccagg
     2401 gctccccggg ctgctctgga tcccaggact ccggctttcg ccgagccgca gcgggatccc
     2461 tgtgcacccg gcgcagccta cccttggtgg tctaaacgga tgctgctggg tgttgcgacc
     2521 caggacgaga tgccttgttt cttttacaat aagtcgttgg aggaatgcca ttaaagtgaa
     2581 ctccccacct ttgcacgctg tgcgggctga gtggttgggg agatgtggcc atggtcttgt
     2641 gctagagatg gcggtacaag agtctgttat gcaagcccgt gtgccaggga tgtgctgggg
     2701 gcggccaccc gctctccagg aaaggcacag ctgaggcact gtggctggct tcggcctcaa
     2761 catcgccccc agccttggag ctctgcagac atgataggaa ggaaactgtc atctgcaggg
     2821 gctttcagca aaatgaaggg ttagattttt atgctgctgc tgatggggtt actaaaggga
     2881 ggggaagagg ccaggtgggc cgctgactgg gccatgggga gaacgtgtgt tcgtactcca
     2941 ggctaaccct gaactcccca tgtgatgcgc gctttgttga atgtgtgtct cggtttcccc
     3001 atctgtaata tgagtcgggg ggaatggtgg tgattcctac ctcacagggc tgttgtgggg
     3061 attaaagtgc tgcgggtgag tgaaggacac atcacgttca gtgtttcaag tacaggccca
     3121 caaaacgggg cacggcaggc ctgagctcag agctgctgca ctgggctttg gatttgttct
     3181 tgtgagtaaa taaaactggc tggtgaatga actgggggac ttctcccttt actttctccc
     3241 ctggctgtgg ccctgagtag caggcttctc gtgaggattc ccgttctctt ctgggagcat
     3301 ggccctgggg ctgcggagaa gccaactgac tgctggagcg aggaaccctg cagctcagaa
     3361 ggtgaacagc tcgtctcaga tgcttgcaga tctttggtta atttagaatt tccagcacag
     3421 acgtggattt ctccagagac ggggtgtgtg cgggtcgctg atgtgaggcc taggggaagc
     3481 aatgagtaaa ctccagggtg ggggaggagc aagggcccgg ggagggctgc agaggggccg
     3541 gggcggggca gggcctgtgc tgcatggtgg cttctgcagg ggagggacag gggagggctg
     3601 cagaggggca gggtgggcag ggcatgtgct gcatggcagc ttctgcacag ccagagcagg
     3661 caccacagga aaagagtcgg ccactggtgc ctgctgtaca gtcacagtgg aggcagagcc
     3721 aaggccaagc agcccctgtg ggttctgggg tttcaccagt ttcatgctcc tcagctcccc
     3781 attcatgccc caccagccct cacttgggac ccatgctccc tcgtggcaca gtcatgatgc
     3841 ctctcaggat tttgtgcctg tggtgctgag tgtcacgtcc ccaaggtcgc cctctggcct
     3901 gcagttcagg gagtgggtgg gagtgaagct gtgctcctgg ggctgcagac agatccactt
     3961 tgcatgtggt acctgctggg ttggccctgc aatctccttt tctctgcacc tacctgtgac
     4021 ctccctggga gagcctccca tttcctcacc tgccctgttg ccagcccggg ggaaatcttt
     4081 ttgctctcaa atgaagactg aaaaaataaa gtcagtgtta tgactaataa gtctcttagg
     4141 aactgtttta caaaatgctt aagaataaaa agtgattttc agcttttata ggtgatgttt
     4201 acagtgtttg taaaccaact ggagggatca gtaattggtc ttaaaagatc aggttctctt
     4261 ttaaaaagtt catctgaaac tttatggttt gttactttcg tgatttgcat aggtatttat
     4321 atatacacac acacacaggt tttgcttttg tctcacattc agcacagctg tctgcggccc
     4381 ctgccggcag gtgtggccgc acctggtgtg aagtcacgtg gagtcagtgc tgtggaggga
     4441 agtcctgtgc gggccgggca gtgaggagag cactgattca cgcctgcaga aacgtaacct
     4501 cccctttccg tgtatgcctt ttaccatcat ccaggcgact gctccatcct tttaaattag
     4561 gggtcctgca agccccaagt gacccatagt cagagtatct ggtttggggt aaccgagttg
     4621 gtcttatggg gcaaaaactc aagacactta gaaacatgca aagtacaaaa taatgtgttc
     4681 cctcctgtta ccccagcaag ggtggggctg agctgggaat gacacccagt ggtgtaatga
     4741 gtggaaagtg ctggtcagct gctaaccccc ctccaagcct gctgcacctt cacttctctg
     4801 catccgtctc ctgagtcata actagggact tggctagatg acctcagaga cctcgtccac
     4861 ctctgaattc tctgttttta agcccatagt tcaaactttg gctttgatct ctgaagtctt
     4921 ctgggtttgg aaatacggga agtcagcttt gaatccattg cttctctaaa acatcagggc
     4981 tagtggaact aacccacttg agggtgacct caagggcttt aggcagccat ggggctgggg
     5041 ccatactgct ctcaggctgg gcagtatctt cattgtggtt ctcagacagc cttttttact
     5101 caaacatgag actttccacc caccagttag ggaacttttt tttttttttt aaacacggag
     5161 tttcactctt gttgcccatg ctggagtgca atggcacaat ctcagctcca ctgcaacctc
     5221 tacctcccgg gttcaagtga ttcttctgcc tcagcctcca agtagctgag attacaggca
     5281 tgcaccacca cacccagcta attttttgta tttagtagag actgggtttc accatgttgg
     5341 tcaggctggt ctccaactcc tgacctcagg tgatccactc tccttggcct cccaaagttc
     5401 tgggattaca ggcgtgagcc actgcgccca gccaagaggg aactttttaa taaagctagt
     5461 gggattccct tgacagcaga accagccttg ggacttgatg cttcaccgtg tcagaatccg
     5521 tccctggtgc tgcctttcag agaagaggct tgatgtgtga ggggtcaggg ggagtgctcc
     5581 agatccctga aatgcccttc ccagaccagt gacaggcatt tttgggtgcc atcgtgtgac
     5641 cttcacggca cagcaaacag tgggcactaa ccctgttgtc ctgtggaaat ctgggtctct
     5701 gaatcctgcc atttccttcc tggaattggc caggaacttg attttctggg ctattgcttg
     5761 ctagtgtccc ctgatgcatg aaggatcccc ccatgtcata ggtcccacct gcctgctgtg
     5821 catcccgggt ggccagactc ggcttctcca ggtgcacttg tcccaggtgg cccggtccgt
     5881 aggctggaag ggcagctgca ggtgcactgc ctcgcggaca ggttaggata tggccacgca
     5941 gccatccatc ttctacagca cgcacacccc actctctccc ccagtcaata tgtctctctc
     6001 cgatgggaaa gttaataaat tttgctctag attaaaagta ttgatcattt catttgtaaa
     6061 cgataaataa aaagggggaa cttttcattg cgccaggggt ggcacctggc gtgtgttgcg
     6121 ggggtgattg cgctggctgc cggggggtgg gcttctcata tgcattctgg ccggccagct
     6181 gcattgattt cctattagtc tcccagcacc acccagtaac acatcatttc agtacctgct
     6241 attaatggtc ttttgataaa taatcacttg taagtcaata aatttttatt aaacagtg
//
