LOCUS       NP_116106                434 aa            linear   PRI 01-AUG-2024
DEFINITION  glycerol-3-phosphate acyltransferase 3 [Homo sapiens].
ACCESSION   NP_116106
VERSION     NP_116106.2
DBSOURCE    REFSEQ: accession NM_032717.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (residues 1 to 434)
  AUTHORS   Fan,G., Li,Y., Zong,Y., Suo,X., Jia,Y., Gao,M. and Yang,X.
  TITLE     GPAT3 regulates the synthesis of lipid intermediate LPA and
            exacerbates Kupffer cell inflammation mediated by the ERK signaling
            pathway
  JOURNAL   Cell Death Dis 14 (3), 208 (2023)
   PUBMED   36964139
  REMARK    GeneRIF: GPAT3 regulates the synthesis of lipid intermediate LPA
            and exacerbates Kupffer cell inflammation mediated by the ERK
            signaling pathway.
            Publication Status: Online-Only
REFERENCE   2  (residues 1 to 434)
  AUTHORS   Tanigawa,K., Hayashi,Y., Hama,K., Yamashita,A., Yokoyama,K.,
            Luo,Y., Kawashima,A., Maeda,Y., Nakamura,Y., Harada,A., Kiriya,M.,
            Karasawa,K. and Suzuki,K.
  TITLE     Mycobacterium leprae promotes triacylglycerol de novo synthesis
            through induction of GPAT3 expression in human premonocytic THP-1
            cells
  JOURNAL   PLoS One 16 (3), e0249184 (2021)
   PUBMED   33770127
  REMARK    GeneRIF: Mycobacterium leprae promotes triacylglycerol de novo
            synthesis through induction of GPAT3 expression in human
            premonocytic THP-1 cells.
            Publication Status: Online-Only
REFERENCE   3  (residues 1 to 434)
  AUTHORS   Sim,M.F.M., Persiani,E., Talukder,M.M.U., Mcilroy,G.D., Roumane,A.,
            Edwardson,J.M. and Rochford,J.J.
  TITLE     Oligomers of the lipodystrophy protein seipin may co-ordinate GPAT3
            and AGPAT2 enzymes to facilitate adipocyte differentiation
  JOURNAL   Sci Rep 10 (1), 3259 (2020)
   PUBMED   32094408
  REMARK    GeneRIF: Oligomers of the lipodystrophy protein seipin may
            co-ordinate GPAT3 and AGPAT2 enzymes to facilitate adipocyte
            differentiation.
            Publication Status: Online-Only
REFERENCE   4  (residues 1 to 434)
  AUTHORS   Fan,S.H., Wang,Y.Y., Wu,Z.Y., Zhang,Z.F., Lu,J., Li,M.Q., Shan,Q.,
            Wu,D.M., Sun,C.H., Hu,B. and Zheng,Y.L.
  TITLE     AGPAT9 suppresses cell growth, invasion and metastasis by
            counteracting acidic tumor microenvironment through
            KLF4/LASS2/V-ATPase signaling pathway in breast cancer
  JOURNAL   Oncotarget 6 (21), 18406-18417 (2015)
   PUBMED   26110566
  REMARK    GeneRIF: Data show that 1-acylglycerol-3-phosphate
            O-acyltransferase 9 (AGPAT9) inhibit cell growth by regulating
            expression of KLF4/LASS2/V-ATPase proteins in breast cancer.
REFERENCE   5  (residues 1 to 434)
  AUTHORS   Comuzzie,A.G., Cole,S.A., Laston,S.L., Voruganti,V.S., Haack,K.,
            Gibbs,R.A. and Butte,N.F.
  TITLE     Novel genetic loci identified for the pathophysiology of childhood
            obesity in the Hispanic population
  JOURNAL   PLoS One 7 (12), e51954 (2012)
   PUBMED   23251661
REFERENCE   6  (residues 1 to 434)
  AUTHORS   Cao,J., Li,J.L., Li,D., Tobin,J.F. and Gimeno,R.E.
  TITLE     Molecular identification of microsomal
            acyl-CoA:glycerol-3-phosphate acyltransferase, a key enzyme in de
            novo triacylglycerol synthesis
  JOURNAL   Proc Natl Acad Sci U S A 103 (52), 19695-19700 (2006)
   PUBMED   17170135
REFERENCE   7  (residues 1 to 434)
  AUTHORS   Tang,W., Yuan,J., Chen,X., Gu,X., Luo,K., Li,J., Wan,B., Wang,Y.
            and Yu,L.
  TITLE     Identification of a novel human lysophosphatidic acid
            acyltransferase, LPAAT-theta, which activates mTOR pathway
  JOURNAL   J Biochem Mol Biol 39 (5), 626-635 (2006)
   PUBMED   17002884
  REMARK    GeneRIF: LPAAT-theta gene consisted of 12 exons and 11 introns, and
            mapped to chromosome 4q21.23, was ubiquitously expressed in 18
            human tissues and overexpression of LPAAT-theta can induce
            mTOR-dependent p70S6K and 4EBP1 phosphorylation in HEK293T cells.
REFERENCE   8  (residues 1 to 434)
  AUTHORS   Oh,J.H., Yang,J.O., Hahn,Y., Kim,M.R., Byun,S.S., Jeon,Y.J.,
            Kim,J.M., Song,K.S., Noh,S.M., Kim,S., Yoo,H.S., Kim,Y.S. and
            Kim,N.S.
  TITLE     Transcriptome analysis of human gastric cancer
  JOURNAL   Mamm Genome 16 (12), 942-954 (2005)
   PUBMED   16341674
REFERENCE   9  (residues 1 to 434)
  AUTHORS   Yamada,S., Ohira,M., Horie,H., Ando,K., Takayasu,H., Suzuki,Y.,
            Sugano,S., Hirata,T., Goto,T., Matsunaga,T., Hiyama,E., Hayashi,Y.,
            Ando,H., Suita,S., Kaneko,M., Sasaki,F., Hashizume,K., Ohnuma,N.
            and Nakagawara,A.
  TITLE     Expression profiling and differential screening between
            hepatoblastomas and the corresponding normal livers: identification
            of high expression of the PLK1 oncogene as a poor-prognostic
            indicator of hepatoblastomas
  JOURNAL   Oncogene 23 (35), 5901-5911 (2004)
   PUBMED   15221005
REFERENCE   10 (residues 1 to 434)
  AUTHORS   Clark,H.F., Gurney,A.L., Abaya,E., Baker,K., Baldwin,D., Brush,J.,
            Chen,J., Chow,B., Chui,C., Crowley,C., Currell,B., Deuel,B.,
            Dowd,P., Eaton,D., Foster,J., Grimaldi,C., Gu,Q., Hass,P.E.,
            Heldens,S., Huang,A., Kim,H.S., Klimowski,L., Jin,Y., Johnson,S.,
            Lee,J., Lewis,L., Liao,D., Mark,M., Robbie,E., Sanchez,C.,
            Schoenfeld,J., Seshagiri,S., Simmons,L., Singh,J., Smith,V.,
            Stinson,J., Vagts,A., Vandlen,R., Watanabe,C., Wieand,D., Woods,K.,
            Xie,M.H., Yansura,D., Yi,S., Yu,G., Yuan,J., Zhang,M., Zhang,Z.,
            Goddard,A., Wood,W.I., Godowski,P. and Gray,A.
  TITLE     The secreted protein discovery initiative (SPDI), a large-scale
            effort to identify novel human secreted and transmembrane proteins:
            a bioinformatics assessment
  JOURNAL   Genome Res 13 (10), 2265-2270 (2003)
   PUBMED   12975309
  REMARK    Erratum:[Genome Res. 2003 Dec;13(12):2759]
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC096768.3, DA673378.1, AY358100.1 and BM841009.1.
            
            On Jun 10, 2002 this sequence version replaced NP_116106.1.
            
            Summary: This gene encodes a member of the lysophosphatidic acid
            acyltransferase protein family. The encoded protein is an enzyme
            which catalyzes the conversion of glycerol-3-phosphate to
            lysophosphatidic acid in the synthesis of triacylglycerol. Multiple
            alternatively spliced variants, encoding the same protein, have
            been identified. [provided by RefSeq, Jan 2012].
            
            Transcript Variant: This variant (1) represents the longest
            transcript. Variants 1, 2 and 3 encode the same protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: DQ345298.1, SRR1660809.33345.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000264409.5/ ENSP00000264409.4
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Homo sapiens"
                     /db_xref="taxon:9606"
                     /chromosome="4"
                     /map="4q21.23"
     Protein         1..434
                     /product="glycerol-3-phosphate acyltransferase 3"
                     /EC_number="2.3.1.51"
                     /EC_number="2.3.1.15"
                     /note="1-acylglycerol-3-phosphate O-acyltransferase 8;
                     lysophosphatidic acid acyltransferase theta; MAG-1;
                     GPAT-3; hGPAT3; 1-AGPAT 9; 1-AGP acyltransferase 9; lung
                     cancer metastasis-associated protein 1;
                     acyl-CoA:glycerol-3-phosphate acyltransferase 3;
                     endoplasmic reticulum associated GPAT;
                     1-acyl-sn-glycerol-3-phosphate O-acyltransferase 9; testis
                     secretory sperm-binding protein Li 213e;
                     1-acyl-sn-glycerol-3-phosphate O-acyltransferase 10;
                     1-acylglycerol-3-phosphate O-acyltransferase 9"
                     /calculated_mol_wt=48574
     Site            14..34
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (Q53EU6.2)"
     Site            68
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:18669648,
                     ECO:0007744|PubMed:23186163, ECO:0007744|PubMed:24275569;
                     propagated from UniProtKB/Swiss-Prot (Q53EU6.2)"
     Site            77
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:18669648,
                     ECO:0007744|PubMed:23186163; propagated from
                     UniProtKB/Swiss-Prot (Q53EU6.2)"
     Site            137..157
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (Q53EU6.2)"
     Site            161..181
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (Q53EU6.2)"
     Region          199..408
                     /region_name="LPLAT_LPCAT1-like"
                     /note="Lysophospholipid Acyltransferases (LPLATs) of
                     Glycerophospholipid Biosynthesis: LPCAT1-like; cd07991"
                     /db_xref="CDD:153253"
     Site            order(229,232,234,248..251,304..306)
                     /site_type="active"
                     /note="putative acyl-acceptor binding pocket [active]"
                     /db_xref="CDD:153253"
     Region          229..234
                     /region_name="HXXXXD motif.
                     /evidence=ECO:0000250|UniProtKB:Q9D517"
                     /note="propagated from UniProtKB/Swiss-Prot (Q53EU6.2)"
     CDS             1..434
                     /gene="GPAT3"
                     /gene_synonym="AGPAT 10; AGPAT10; AGPAT8; AGPAT9;
                     HMFN0839; LPAAT-theta; MAG1"
                     /coded_by="NM_032717.5:516..1820"
                     /db_xref="CCDS:CCDS3606.1"
                     /db_xref="GeneID:84803"
                     /db_xref="HGNC:HGNC:28157"
                     /db_xref="MIM:610958"
ORIGIN      
        1 megaelagki lstwltlvlg fillpsvfgv slgiseiymk ilvktlewat iriekgtpke
       61 silknsasvg iiqrdespme kglsglrgrd felsdvfyfs kkgleaived evtqrfssee
      121 lvswnlltrt nvnfqyislr ltmvwvlgvi vrycvllplr vtlafigisl lvigttlvgq
      181 lpdsslknwl selvhltccr icvralsgti hyhnkqyrpq kggicvanht spidvliltt
      241 dgcyamvgqv hgglmgiiqr amvkacphvw fersemkdrh lvtkrlkehi adkkklpili
      301 fpegtcinnt svmmfkkgsf eiggtihpva ikynpqfgda fwnsskynmv syllrmmtsw
      361 aivcdvwymp pmtreegeda vqfanrvksa iaiqggltel pwdgglkrak vkdifkeeqq
      421 knyskmivgn gsls
//
