LOCUS       NM_001321527            2743 bp    mRNA    linear   PRI 22-SEP-2024
DEFINITION  Homo sapiens glycerol-3-phosphate acyltransferase 2, mitochondrial
            (GPAT2), transcript variant 4, mRNA; nuclear gene for mitochondrial
            product.
ACCESSION   NM_001321527
VERSION     NM_001321527.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2743)
  AUTHORS   Morgenstern,M., Peikert,C.D., Lubbert,P., Suppanz,I., Klemm,C.,
            Alka,O., Steiert,C., Naumenko,N., Schendzielorz,A., Melchionda,L.,
            Muhlhauser,W.W.D., Knapp,B., Busch,J.D., Stiller,S.B.,
            Dannenmaier,S., Lindau,C., Licheva,M., Eickhorst,C., Galbusera,R.,
            Zerbes,R.M., Ryan,M.T., Kraft,C., Kozjak-Pavlovic,V., Drepper,F.,
            Dennerlein,S., Oeljeklaus,S., Pfanner,N., Wiedemann,N. and
            Warscheid,B.
  TITLE     Quantitative high-confidence human mitochondrial proteome and its
            dynamics in cellular context
  JOURNAL   Cell Metab 33 (12), 2464-2483 (2021)
   PUBMED   34800366
REFERENCE   2  (bases 1 to 2743)
  AUTHORS   Pellon-Maison,M., Montanaro,M.A., Lacunza,E., Garcia-Fabiani,M.B.,
            Soler-Gerino,M.C., Cattaneo,E.R., Quiroga,I.Y., Abba,M.C.,
            Coleman,R.A. and Gonzalez-Baro,M.R.
  TITLE     Glycerol-3-phosphate acyltranferase-2 behaves as a cancer testis
            gene and promotes growth and tumorigenicity of the breast cancer
            MDA-MB-231 cell line
  JOURNAL   PLoS One 9 (6), e100896 (2014)
   PUBMED   24967918
  REMARK    GeneRIF: GPAT2 as a cancer testis gene
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2743)
  AUTHORS   Shiromoto,Y., Kuramochi-Miyagawa,S., Daiba,A., Chuma,S.,
            Katanaya,A., Katsumata,A., Nishimura,K., Ohtaka,M., Nakanishi,M.,
            Nakamura,T., Yoshinaga,K., Asada,N., Nakamura,S., Yasunaga,T.,
            Kojima-Kita,K., Itou,D., Kimura,T. and Nakano,T.
  TITLE     GPAT2, a mitochondrial outer membrane protein, in piRNA biogenesis
            in germline stem cells
  JOURNAL   RNA 19 (6), 803-810 (2013)
   PUBMED   23611983
REFERENCE   4  (bases 1 to 2743)
  AUTHORS   Gimeno,R.E. and Cao,J.
  TITLE     Thematic review series: glycerolipids. Mammalian
            glycerol-3-phosphate acyltransferases: new genes for an old
            activity
  JOURNAL   J Lipid Res 49 (10), 2079-2088 (2008)
   PUBMED   18658143
  REMARK    Review article
REFERENCE   5  (bases 1 to 2743)
  AUTHORS   Chen,Y.Q., Kuo,M.S., Li,S., Bui,H.H., Peake,D.A., Sanders,P.E.,
            Thibodeaux,S.J., Chu,S., Qian,Y.W., Zhao,Y., Bredt,D.S.,
            Moller,D.E., Konrad,R.J., Beigneux,A.P., Young,S.G. and Cao,G.
  TITLE     AGPAT6 is a novel microsomal glycerol-3-phosphate acyltransferase
  JOURNAL   J Biol Chem 283 (15), 10048-10057 (2008)
   PUBMED   18238778
REFERENCE   6  (bases 1 to 2743)
  AUTHORS   Wang,S., Lee,D.P., Gong,N., Schwerbrock,N.M., Mashek,D.G.,
            Gonzalez-Baro,M.R., Stapleton,C., Li,L.O., Lewin,T.M. and
            Coleman,R.A.
  TITLE     Cloning and functional characterization of a novel mitochondrial
            N-ethylmaleimide-sensitive glycerol-3-phosphate acyltransferase
            (GPAT2)
  JOURNAL   Arch Biochem Biophys 465 (2), 347-358 (2007)
   PUBMED   17689486
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC013272.5.
            
            On Jan 7, 2021 this sequence version replaced NM_001321527.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR18074968.2870743.1,
                                           SRR14038192.600030.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: inferred from homology
            MANE Ensembl match                 :: ENST00000434632.6/
                                                  ENSP00000389395.2
            RefSeq Select criteria             :: based on manual assertion,
                                                  conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-64                AC013272.5         67879-67942
            65-187              AC013272.5         70376-70498
            188-301             AC013272.5         70649-70762
            302-375             AC013272.5         71461-71534
            376-480             AC013272.5         71947-72051
            481-609             AC013272.5         72261-72389
            610-709             AC013272.5         74705-74804
            710-843             AC013272.5         75243-75376
            844-943             AC013272.5         75689-75788
            944-1156            AC013272.5         75886-76098
            1157-1279           AC013272.5         76490-76612
            1280-1362           AC013272.5         76783-76865
            1363-1481           AC013272.5         77192-77310
            1482-1552           AC013272.5         77952-78022
            1553-1811           AC013272.5         78110-78368
            1812-1960           AC013272.5         78458-78606
            1961-2038           AC013272.5         78795-78872
            2039-2170           AC013272.5         79355-79486
            2171-2291           AC013272.5         79569-79689
            2292-2357           AC013272.5         79772-79837
            2358-2413           AC013272.5         80072-80127
            2414-2743           AC013272.5         80520-80849
FEATURES             Location/Qualifiers
     source          1..2743
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q11.2"
     gene            1..2743
                     /gene="GPAT2"
                     /gene_synonym="CT123"
                     /note="glycerol-3-phosphate acyltransferase 2,
                     mitochondrial"
                     /db_xref="GeneID:150763"
                     /db_xref="HGNC:HGNC:27168"
                     /db_xref="MIM:616431"
     exon            1..64
                     /gene="GPAT2"
                     /gene_synonym="CT123"
                     /inference="alignment:Splign:2.1.0"
     exon            65..187
                     /gene="GPAT2"
                     /gene_synonym="CT123"
                     /inference="alignment:Splign:2.1.0"
     CDS             125..2530
                     /gene="GPAT2"
                     /gene_synonym="CT123"
                     /EC_number="2.3.1.51"
                     /EC_number="2.3.1.15"
                     /note="isoform 2 is encoded by transcript variant 4;
                     cancer/testis antigen 123; 1-acylglycerol-3-phosphate
                     O-acyltransferase GPAT2"
                     /codon_start=1
                     /product="glycerol-3-phosphate acyltransferase 2,
                     mitochondrial isoform 2"
                     /protein_id="NP_001308456.1"
                     /db_xref="CCDS:CCDS92811.1"
                     /db_xref="GeneID:150763"
                     /db_xref="HGNC:HGNC:27168"
                     /db_xref="MIM:616431"
                     /translation="MATMLEGRCQTQPRSSPSGREASLWSSGFGMKLEAVTPFLGKYR
                     PFVGRCCQTCTPKSWESLFHRSITDLGFCNVILVKEENTRFRGWLVRRLCYFLWSLEQ
                     HIPPCQDVPQKIMESTGVQNLLSGRVPGGTGEGQVPDLVKKEVQRILGHIQAPPRPFL
                     VRLFSWALLRFLNCLFLNVQLHKGQMKMVQKAAQAGLPLVLLSTHKTLLDGILLPFML
                     LSQGLGVLRVAWDSRACSPALRALLRKLGGLFLPPEASLSLDSSEGLLARAVVQAVIE
                     QLLVSGQPLLIFLEEPPGALGPRLSALGQAWVGFVVQAVQVGIVPDALLVPVAVTYDL
                     VPDAPCDIDHASAPLGLWTGALAVLRSLWSRWGCSHRICSRVHLAQPFSLQEYIVSAR
                     SCWGGRQTLEQLLQPIVLGQCTAVPDTEKEQEWTPITGPLLALKEEDQLLVRRLSCHV
                     LSASVGSSAVMSTAIMATLLLFKHQKGVFLSQLLGEFSWLTEEILLRGFDVGFSGQLR
                     SLLQHSLSLLRAHVALLRIRQGDLLVVPQPGPGLTHLAQLSAELLPVFLSEAVGACAV
                     RGLLAGRVPPQGPWELQGILLLSQNELYRQILLLMHLLPQDLLLLKPCQSSYCYCQEV
                     LDRLIQCGLLVAEETPGSRPACDTGRQRLSRKLLWKPSGDFTDSDSDDFGEADGRYFR
                     LSQQSHCPDFFLFLCRLLSPLLKAFAQAAAFLRQGQLPDTELGYTEQLFQFLQATAQE
                     EGIFECADPKLAISAVWTFRDLGVLQQTPSPAGPRLHLSPTFASLDNQEKLEQFIRQF
                     ICS"
     exon            188..301
                     /gene="GPAT2"
                     /gene_synonym="CT123"
                     /inference="alignment:Splign:2.1.0"
     exon            302..375
                     /gene="GPAT2"
                     /gene_synonym="CT123"
                     /inference="alignment:Splign:2.1.0"
     exon            376..480
                     /gene="GPAT2"
                     /gene_synonym="CT123"
                     /inference="alignment:Splign:2.1.0"
     exon            481..609
                     /gene="GPAT2"
                     /gene_synonym="CT123"
                     /inference="alignment:Splign:2.1.0"
     exon            610..709
                     /gene="GPAT2"
                     /gene_synonym="CT123"
                     /inference="alignment:Splign:2.1.0"
     exon            710..843
                     /gene="GPAT2"
                     /gene_synonym="CT123"
                     /inference="alignment:Splign:2.1.0"
     exon            844..943
                     /gene="GPAT2"
                     /gene_synonym="CT123"
                     /inference="alignment:Splign:2.1.0"
     exon            944..1156
                     /gene="GPAT2"
                     /gene_synonym="CT123"
                     /inference="alignment:Splign:2.1.0"
     exon            1157..1279
                     /gene="GPAT2"
                     /gene_synonym="CT123"
                     /inference="alignment:Splign:2.1.0"
     exon            1280..1362
                     /gene="GPAT2"
                     /gene_synonym="CT123"
                     /inference="alignment:Splign:2.1.0"
     exon            1363..1481
                     /gene="GPAT2"
                     /gene_synonym="CT123"
                     /inference="alignment:Splign:2.1.0"
     exon            1482..1552
                     /gene="GPAT2"
                     /gene_synonym="CT123"
                     /inference="alignment:Splign:2.1.0"
     exon            1553..1811
                     /gene="GPAT2"
                     /gene_synonym="CT123"
                     /inference="alignment:Splign:2.1.0"
     exon            1812..1960
                     /gene="GPAT2"
                     /gene_synonym="CT123"
                     /inference="alignment:Splign:2.1.0"
     exon            1961..2038
                     /gene="GPAT2"
                     /gene_synonym="CT123"
                     /inference="alignment:Splign:2.1.0"
     exon            2039..2170
                     /gene="GPAT2"
                     /gene_synonym="CT123"
                     /inference="alignment:Splign:2.1.0"
     exon            2171..2291
                     /gene="GPAT2"
                     /gene_synonym="CT123"
                     /inference="alignment:Splign:2.1.0"
     exon            2292..2357
                     /gene="GPAT2"
                     /gene_synonym="CT123"
                     /inference="alignment:Splign:2.1.0"
     exon            2358..2413
                     /gene="GPAT2"
                     /gene_synonym="CT123"
                     /inference="alignment:Splign:2.1.0"
     exon            2414..2743
                     /gene="GPAT2"
                     /gene_synonym="CT123"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2700..2705
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GPAT2"
                     /gene_synonym="CT123"
                     /note="hexamer: AACAAG"
     polyA_site      2743
                     /gene="GPAT2"
                     /gene_synonym="CT123"
                     /note="major polyA site"
ORIGIN      
        1 agtccacgcc ttgtggcggc tttgcggagc tgctgctttg gcgggagttg gaagctggtg
       61 tgaggtttct gtggggagaa ggagagtgcc agaggtgact ggttcatggt tcttctaggc
      121 tctcatggcc accatgttgg aaggcagatg ccaaactcag ccaaggagca gccccagtgg
      181 ccgagaggct agcctgtggt cgtcaggctt tgggatgaag ctggaggctg tcactccatt
      241 cctggggaag tatcgcccct ttgtgggtcg ctgttgccag acctgcaccc ccaagagctg
      301 ggagtccctc ttccacagaa gcataacgga cctaggcttc tgcaatgtga tcctggtgaa
      361 ggaggagaac acaaggtttc ggggctggct ggttcggagg ctctgctatt tcctgtggtc
      421 cctggagcag cacatccccc cctgccagga tgtcccacag aagatcatgg aaagcaccgg
      481 ggtgcagaac ctcctctcag ggagggtccc aggaggcact ggggaaggcc aggtgcctga
      541 ccttgtgaag aaggaggtac agcgcatcct gggtcacatc caggccccac cccgtccctt
      601 cctggtcagg ctgttcagct gggcgctgct gaggttcctg aactgcctgt tcctgaatgt
      661 gcagctccac aagggtcaga tgaagatggt ccagaaggcc gcccaggcag gcttgccgct
      721 tgtcctcctc tctactcaca aaaccctcct ggatgggatc ctgctgccct ttatgctgct
      781 ctcccagggc ctgggtgtgc ttcgtgtggc ctgggactcc cgcgcctgct cccctgccct
      841 cagagctctg ctgaggaagc ttggggggct tttcctgccc ccagaggcca gcctctccct
      901 ggacagctct gaggggctcc ttgccagggc tgtggtccag gcggtcatag agcagctgct
      961 ggttagtggg cagcccctgc tcatcttcct ggaggaacct cctggggctc tggggccacg
     1021 gctgtcagcc ctgggccagg cttgggtggg gtttgtggtg caggcagtcc aggtgggcat
     1081 cgtcccagat gctctgctgg taccagtggc cgtcacctat gacctggttc cggatgcacc
     1141 gtgtgacata gaccatgcct cggcccccct ggggctgtgg acaggagctc tggctgtcct
     1201 acgtagcttg tggagccgct ggggctgcag ccaccggatc tgctcccggg tgcacctagc
     1261 tcagcccttt tccctgcagg aatacatcgt cagtgccaga agctgctggg gcggcagaca
     1321 gaccctggag cagctactgc agcccatcgt gctgggccaa tgtactgctg tcccagacac
     1381 tgagaaggag caggagtgga cccccataac tgggcctctc ctggccctca aggaagagga
     1441 ccagctcctg gtcaggagac tgagctgtca tgtcctgagt gccagtgtag ggagctctgc
     1501 ggtgatgagc acggccatta tggcaacgct gctgctcttc aagcatcaga agggtgtgtt
     1561 cctgtcgcag ctcctggggg agttctcctg gctgacggag gagatactgt tgcgtggctt
     1621 tgatgtaggc ttctctgggc agctgcggag cctgctgcag cactcactga gcctgctgcg
     1681 ggcgcacgtg gccctgctgc gcatccgtca gggtgacttg ctggtggtgc cgcagcctgg
     1741 cccaggcctc acacacctgg cacaactgag tgctgagctg ctgcccgtct tcctgagcga
     1801 ggctgtgggc gcctgtgcag tgcgggggct gctggcaggc agagtgccgc cccaggggcc
     1861 ctgggagctg cagggcatat tgctgctgag ccagaatgag ctgtaccgcc agatcctgct
     1921 gctgatgcac ctgctgccgc aagacctgct gctgctaaag ccctgccagt cttcctactg
     1981 ctactgtcag gaggtgctgg accggctcat ccaatgcggg ctcctggttg ctgaggagac
     2041 cccaggctcc cggccagcct gtgacacagg gcgacagcga ttgagcagaa agctgctgtg
     2101 gaaaccgagt ggggacttta ctgatagtga cagtgatgac ttcggagagg ctgacggccg
     2161 gtacttcagg ctcagccagc agtcacactg cccagatttc tttcttttcc tctgccgcct
     2221 gctcagcccg ctgctcaagg cctttgcaca ggctgccgcc ttcctccgcc agggccagct
     2281 gcccgatact gagttgggct acacagagca gctgttccag ttcctgcagg ccaccgccca
     2341 ggaagaaggg atcttcgagt gtgcggaccc aaagctcgcc atcagtgctg tctggacctt
     2401 cagagaccta ggggttctgc agcagacgcc gagccctgca ggccccaggc tccacctgtc
     2461 ccctactttt gccagcctgg acaatcagga aaaactagaa cagttcatcc ggcagttcat
     2521 ttgtagctag aactgtgagg aggagcctgt gctgagactt ctcagcccca gaacacagct
     2581 gtgtcctaga gccagaagat ggagaggagg ctgcaaaccc ttagctgctc tataaatata
     2641 atcattgagg cttgattgtc ccttgccatc tcttgctttt tcccttcttt gatgtgataa
     2701 acaaggggac gagacgagtt gtcttttccc cagcccagca gca
//
