LOCUS       NM_001244949            6372 bp    mRNA    linear   PRI 06-DEC-2024
DEFINITION  Homo sapiens glycerol-3-phosphate acyltransferase, mitochondrial
            (GPAM), transcript variant 1, mRNA; nuclear gene for mitochondrial
            product.
ACCESSION   NM_001244949
VERSION     NM_001244949.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6372)
  AUTHORS   Johnson,Z.L., Ammirati,M., Wasilko,D.J., Chang,J.S., Noell,S.,
            Foley,T.L., Yoon,H., Smith,K., Asano,S., Hales,K., Wan,M., Yang,Q.,
            Piotrowski,M.A., Farley,K.A., Gilbert,T., Aschenbrenner,L.M.,
            Fennell,K.F., Dutra,J.K., Xu,M., Guo,C., Varghese,A.E.,
            Bellenger,J., Quinn,A., Am Ende,C.W., West,G.M., Griffor,M.C.,
            Bennett,D., Calabrese,M., Steppan,C.M., Han,S. and Wu,H.
  TITLE     Structural basis of the acyl-transfer mechanism of human GPAT1
  JOURNAL   Nat Struct Mol Biol 30 (1), 22-30 (2023)
   PUBMED   36522428
  REMARK    GeneRIF: Structural basis of the acyl-transfer mechanism of human
            GPAT1.
REFERENCE   2  (bases 1 to 6372)
  AUTHORS   Irifune,H.
  TITLE     [Identification of GPAT1-dependent mitochondrial metabolism as a
            novel therapeutic target for AML]
  JOURNAL   Rinsho Ketsueki 63 (5), 353-362 (2022)
   PUBMED   35662157
  REMARK    GeneRIF: [Identification of GPAT1-dependent mitochondrial
            metabolism as a novel therapeutic target for AML].
REFERENCE   3  (bases 1 to 6372)
  AUTHORS   Morgenstern,M., Peikert,C.D., Lubbert,P., Suppanz,I., Klemm,C.,
            Alka,O., Steiert,C., Naumenko,N., Schendzielorz,A., Melchionda,L.,
            Muhlhauser,W.W.D., Knapp,B., Busch,J.D., Stiller,S.B.,
            Dannenmaier,S., Lindau,C., Licheva,M., Eickhorst,C., Galbusera,R.,
            Zerbes,R.M., Ryan,M.T., Kraft,C., Kozjak-Pavlovic,V., Drepper,F.,
            Dennerlein,S., Oeljeklaus,S., Pfanner,N., Wiedemann,N. and
            Warscheid,B.
  TITLE     Quantitative high-confidence human mitochondrial proteome and its
            dynamics in cellular context
  JOURNAL   Cell Metab 33 (12), 2464-2483 (2021)
   PUBMED   34800366
REFERENCE   4  (bases 1 to 6372)
  AUTHORS   Hakim,A., Moll,M., Brancale,J., Liu,J., Lasky-Su,J.A.,
            Silverman,E.K., Vilarinho,S., Jiang,Z.G., Pita-Juarez,Y.H.,
            Vlachos,I.S., Zhang,X., Aberg,F., Afdhal,N.H., Hobbs,B.D. and
            Cho,M.H.
  TITLE     Genetic Variation in the Mitochondrial Glycerol-3-Phosphate
            Acyltransferase Is Associated With Liver Injury
  JOURNAL   Hepatology 74 (6), 3394-3408 (2021)
   PUBMED   34216018
  REMARK    GeneRIF: Genetic Variation in the Mitochondrial
            Glycerol-3-Phosphate Acyltransferase Is Associated With Liver
            Injury.
REFERENCE   5  (bases 1 to 6372)
  AUTHORS   Jamialahmadi,O., Mancina,R.M., Ciociola,E., Tavaglione,F.,
            Luukkonen,P.K., Baselli,G., Malvestiti,F., Thuillier,D.,
            Raverdy,V., Mannisto,V., Pipitone,R.M., Pennisi,G., Prati,D.,
            Spagnuolo,R., Petta,S., Pihlajamaki,J., Pattou,F., Yki-Jarvinen,H.,
            Valenti,L. and Romeo,S.
  TITLE     Exome-Wide Association Study on Alanine Aminotransferase Identifies
            Sequence Variants in the GPAM and APOE Associated With Fatty Liver
            Disease
  JOURNAL   Gastroenterology 160 (5), 1634-1646 (2021)
   PUBMED   33347879
  REMARK    GeneRIF: Exome-Wide Association Study on Alanine Aminotransferase
            Identifies Sequence Variants in the GPAM and APOE Associated With
            Fatty Liver Disease.
REFERENCE   6  (bases 1 to 6372)
  AUTHORS   Deloukas,P., Earthrowl,M.E., Grafham,D.V., Rubenfield,M.,
            French,L., Steward,C.A., Sims,S.K., Jones,M.C., Searle,S.,
            Scott,C., Howe,K., Hunt,S.E., Andrews,T.D., Gilbert,J.G.,
            Swarbreck,D., Ashurst,J.L., Taylor,A., Battles,J., Bird,C.P.,
            Ainscough,R., Almeida,J.P., Ashwell,R.I., Ambrose,K.D.,
            Babbage,A.K., Bagguley,C.L., Bailey,J., Banerjee,R., Bates,K.,
            Beasley,H., Bray-Allen,S., Brown,A.J., Brown,J.Y., Burford,D.C.,
            Burrill,W., Burton,J., Cahill,P., Camire,D., Carter,N.P.,
            Chapman,J.C., Clark,S.Y., Clarke,G., Clee,C.M., Clegg,S., Corby,N.,
            Coulson,A., Dhami,P., Dutta,I., Dunn,M., Faulkner,L., Frankish,A.,
            Frankland,J.A., Garner,P., Garnett,J., Gribble,S., Griffiths,C.,
            Grocock,R., Gustafson,E., Hammond,S., Harley,J.L., Hart,E.,
            Heath,P.D., Ho,T.P., Hopkins,B., Horne,J., Howden,P.J., Huckle,E.,
            Hynds,C., Johnson,C., Johnson,D., Kana,A., Kay,M., Kimberley,A.M.,
            Kershaw,J.K., Kokkinaki,M., Laird,G.K., Lawlor,S., Lee,H.M.,
            Leongamornlert,D.A., Laird,G., Lloyd,C., Lloyd,D.M., Loveland,J.,
            Lovell,J., McLaren,S., McLay,K.E., McMurray,A.,
            Mashreghi-Mohammadi,M., Matthews,L., Milne,S., Nickerson,T.,
            Nguyen,M., Overton-Larty,E., Palmer,S.A., Pearce,A.V., Peck,A.I.,
            Pelan,S., Phillimore,B., Porter,K., Rice,C.M., Rogosin,A.,
            Ross,M.T., Sarafidou,T., Sehra,H.K., Shownkeen,R., Skuce,C.D.,
            Smith,M., Standring,L., Sycamore,N., Tester,J., Thorpe,A.,
            Torcasso,W., Tracey,A., Tromans,A., Tsolas,J., Wall,M., Walsh,J.,
            Wang,H., Weinstock,K., West,A.P., Willey,D.L., Whitehead,S.L.,
            Wilming,L., Wray,P.W., Young,L., Chen,Y., Lovering,R.C.,
            Moschonas,N.K., Siebert,R., Fechtel,K., Bentley,D., Durbin,R.,
            Hubbard,T., Doucette-Stamm,L., Beck,S., Smith,D.R. and Rogers,J.
  TITLE     The DNA sequence and comparative analysis of human chromosome 10
  JOURNAL   Nature 429 (6990), 375-381 (2004)
   PUBMED   15164054
REFERENCE   7  (bases 1 to 6372)
  AUTHORS   Igal,R.A., Wang,S., Gonzalez-Baro,M. and Coleman,R.A.
  TITLE     Mitochondrial glycerol phosphate acyltransferase directs the
            incorporation of exogenous fatty acids into triacylglycerol
  JOURNAL   J Biol Chem 276 (45), 42205-42212 (2001)
   PUBMED   11546763
REFERENCE   8  (bases 1 to 6372)
  AUTHORS   Lehner,R. and Kuksis,A.
  TITLE     Biosynthesis of triacylglycerols
  JOURNAL   Prog Lipid Res 35 (2), 169-201 (1996)
   PUBMED   8944226
  REMARK    Review article
REFERENCE   9  (bases 1 to 6372)
  AUTHORS   Skorve,J., Ruyter,B., Rustan,A.C., Christiansen,E.N., Drevon,C.A.
            and Berge,R.K.
  TITLE     Effect of 3- and 4-thia-substituted fatty acids on glycerolipid
            metabolism and mitochondrial beta-oxidation in rat liver
  JOURNAL   Biochem Pharmacol 40 (9), 2005-2012 (1990)
   PUBMED   2242030
REFERENCE   10 (bases 1 to 6372)
  AUTHORS   Thomas,P.D. and Poznansky,M.J.
  TITLE     Lipid peroxidation inactivates rat liver microsomal
            glycerol-3-phosphate acyl transferase. Effect of iron and copper
            salts and carbon tetrachloride
  JOURNAL   J Biol Chem 265 (5), 2684-2691 (1990)
   PUBMED   2303421
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA802170.1, AL833093.2,
            BC030783.1, BX647451.1, DA728196.1, AB046780.1 and AL833061.2.
            
            On Nov 23, 2018 this sequence version replaced NM_001244949.1.
            
            Summary: This gene encodes a mitochondrial enzyme which prefers
            saturated fatty acids as its substrate for the synthesis of
            glycerolipids. This metabolic pathway's first step is catalyzed by
            the encoded enzyme. Two forms for this enzyme exist, one in the
            mitochondria and one in the endoplasmic reticulum. Two
            alternatively spliced transcript variants have been described for
            this gene. [provided by RefSeq, Oct 2011].
            
            Transcript Variant: This variant (1) represents the longer
            transcript. Variants 1 and 2 encode the same protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AL833061.2, SRR1660807.155407.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            MANE Ensembl match                 :: ENST00000348367.9/
                                                  ENSP00000265276.4
            RefSeq Select criteria             :: based on conservation,
                                                  longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2                 DA802170.1         15-16
            3-760               AL833093.2         2-759
            761-2570            BC030783.1         739-2548
            2571-4266           AL833093.2         2570-4265
            4267-5122           BX647451.1         4245-5100
            5123-5627           DA728196.1         214-718
            5628-6366           AB046780.1         4931-5669
            6367-6372           AL833061.2         6362-6367
FEATURES             Location/Qualifiers
     source          1..6372
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="10"
                     /map="10q25.2"
     gene            1..6372
                     /gene="GPAM"
                     /gene_synonym="GPAT; GPAT1"
                     /note="glycerol-3-phosphate acyltransferase,
                     mitochondrial"
                     /db_xref="GeneID:57678"
                     /db_xref="HGNC:HGNC:24865"
                     /db_xref="MIM:602395"
     exon            1..73
                     /gene="GPAM"
                     /gene_synonym="GPAT; GPAT1"
                     /inference="alignment:Splign:2.1.0"
     exon            74..171
                     /gene="GPAM"
                     /gene_synonym="GPAT; GPAT1"
                     /inference="alignment:Splign:2.1.0"
     exon            172..302
                     /gene="GPAM"
                     /gene_synonym="GPAT; GPAT1"
                     /inference="alignment:Splign:2.1.0"
     CDS             201..2687
                     /gene="GPAM"
                     /gene_synonym="GPAT; GPAT1"
                     /EC_number="2.3.1.15"
                     /note="glycerol-3-phosphate acyltransferase 1,
                     mitochondrial; GPAT-1"
                     /codon_start=1
                     /product="glycerol-3-phosphate acyltransferase 1,
                     mitochondrial precursor"
                     /protein_id="NP_001231878.1"
                     /db_xref="CCDS:CCDS7570.1"
                     /db_xref="GeneID:57678"
                     /db_xref="HGNC:HGNC:24865"
                     /db_xref="MIM:602395"
                     /translation="MDESALTLGTIDVSYLPHSSEYSVGRCKHTSEEWGECGFRPTIF
                     RSATLKWKESLMSRKRPFVGRCCYSCTPQSWDKFFNPSIPSLGLRNVIYINETHTRHR
                     GWLARRLSYVLFIQERDVHKGMFATNVTENVLNSSRVQEAIAEVAAELNPDGSAQQQS
                     KAVNKVKKKAKRILQEMVATVSPAMIRLTGWVLLKLFNSFFWNIQIHKGQLEMVKAAT
                     ETNLPLLFLPVHRSHIDYLLLTFILFCHNIKAPYIASGNNLNIPIFSTLIHKLGGFFI
                     RRRLDETPDGRKDVLYRALLHGHIVELLRQQQFLEIFLEGTRSRSGKTSCARAGLLSV
                     VVDTLSTNVIPDILIIPVGISYDRIIEGHYNGEQLGKPKKNESLWSVARGVIRMLRKN
                     YGCVRVDFAQPFSLKEYLESQSQKPVSALLSLEQALLPAILPSRPSDAADEGRDTSIN
                     ESRNATDESLRRRLIANLAEHILFTASKSCAIMSTHIVACLLLYRHRQGIDLSTLVED
                     FFVMKEEVLARDFDLGFSGNSEDVVMHAIQLLGNCVTITHTSRNDEFFITPSTTVPSV
                     FELNFYSNGVLHVFIMEAIIACSLYAVLNKRGLGGPTSTPPNLISQEQLVRKAASLCY
                     LLSNEGTISLPCQTFYQVCHETVGKFIQYGILTVAEHDDQEDISPSLAEQQWDKKLPE
                     PLSWRSDEEDEDSDFGEEQRDCYLKVSQSKEHQQFITFLQRLLGPLLEAYSSAAIFVH
                     NFSGPVPEPEYLQKLHKYLITRTERNVAVYAESATYCLVKNAVKMFKDIGVFKETKQK
                     RVSVLELSSTFLPQCNRQKLLEYILSFVVL"
     exon            303..425
                     /gene="GPAM"
                     /gene_synonym="GPAT; GPAT1"
                     /inference="alignment:Splign:2.1.0"
     exon            426..499
                     /gene="GPAM"
                     /gene_synonym="GPAT; GPAT1"
                     /inference="alignment:Splign:2.1.0"
     exon            500..613
                     /gene="GPAM"
                     /gene_synonym="GPAT; GPAT1"
                     /inference="alignment:Splign:2.1.0"
     exon            614..760
                     /gene="GPAM"
                     /gene_synonym="GPAT; GPAT1"
                     /inference="alignment:Splign:2.1.0"
     exon            761..857
                     /gene="GPAM"
                     /gene_synonym="GPAT; GPAT1"
                     /inference="alignment:Splign:2.1.0"
     exon            858..994
                     /gene="GPAM"
                     /gene_synonym="GPAT; GPAT1"
                     /inference="alignment:Splign:2.1.0"
     exon            995..1094
                     /gene="GPAM"
                     /gene_synonym="GPAT; GPAT1"
                     /inference="alignment:Splign:2.1.0"
     exon            1095..1307
                     /gene="GPAM"
                     /gene_synonym="GPAT; GPAT1"
                     /inference="alignment:Splign:2.1.0"
     exon            1308..1421
                     /gene="GPAM"
                     /gene_synonym="GPAT; GPAT1"
                     /inference="alignment:Splign:2.1.0"
     exon            1422..1507
                     /gene="GPAM"
                     /gene_synonym="GPAT; GPAT1"
                     /inference="alignment:Splign:2.1.0"
     exon            1508..1623
                     /gene="GPAM"
                     /gene_synonym="GPAT; GPAT1"
                     /inference="alignment:Splign:2.1.0"
     exon            1624..1694
                     /gene="GPAM"
                     /gene_synonym="GPAT; GPAT1"
                     /inference="alignment:Splign:2.1.0"
     exon            1695..1959
                     /gene="GPAM"
                     /gene_synonym="GPAT; GPAT1"
                     /inference="alignment:Splign:2.1.0"
     exon            1960..2102
                     /gene="GPAM"
                     /gene_synonym="GPAT; GPAT1"
                     /inference="alignment:Splign:2.1.0"
     exon            2103..2180
                     /gene="GPAM"
                     /gene_synonym="GPAT; GPAT1"
                     /inference="alignment:Splign:2.1.0"
     exon            2181..2321
                     /gene="GPAM"
                     /gene_synonym="GPAT; GPAT1"
                     /inference="alignment:Splign:2.1.0"
     exon            2322..2511
                     /gene="GPAM"
                     /gene_synonym="GPAT; GPAT1"
                     /inference="alignment:Splign:2.1.0"
     exon            2512..2570
                     /gene="GPAM"
                     /gene_synonym="GPAT; GPAT1"
                     /inference="alignment:Splign:2.1.0"
     exon            2571..6372
                     /gene="GPAM"
                     /gene_synonym="GPAT; GPAT1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6351..6356
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GPAM"
                     /gene_synonym="GPAT; GPAT1"
                     /note="hexamer: AATAAA"
     polyA_site      6372
                     /gene="GPAM"
                     /gene_synonym="GPAT; GPAT1"
                     /note="major polyA site"
ORIGIN      
        1 gcgccactgc agctggcatt ggccgggact ggaagtgcgg gcttctgcag cagccgaagc
       61 tggagctgct agggcagcag cggctcccct gttgtatgga cattctgcac ccgaaactga
      121 tagctgagtc ctgaagtttt atgttatgaa acagaagaac tttcatccca gcacatgatt
      181 tgggaattac actttgtgac atggatgaat ctgcactgac ccttggtaca atagatgttt
      241 cttatctgcc acattcatca gaatacagtg ttggtcgatg taagcacaca agtgaggaat
      301 ggggtgagtg tggctttaga cccaccatct tcagatctgc aactttaaaa tggaaagaaa
      361 gcctaatgag tcggaaaagg ccatttgttg gaagatgttg ttactcctgc actccccaga
      421 gctgggacaa atttttcaac cccagtatcc cgtctttggg tttgcggaat gttatttata
      481 tcaatgaaac tcacacaaga caccgcggat ggcttgcaag acgcctttct tacgttcttt
      541 ttattcaaga gcgagatgtg cataagggca tgtttgccac caatgtgact gaaaatgtgc
      601 tgaacagcag tagagtacaa gaggcaattg cagaagtggc tgctgaatta aaccctgatg
      661 gttctgccca gcagcaatca aaagccgtta acaaagtgaa aaagaaagct aaaaggattc
      721 ttcaagaaat ggttgccact gtctcaccgg caatgatcag actgactggg tgggtgctgc
      781 taaaactgtt caacagcttc ttttggaaca ttcaaattca caaaggtcaa cttgagatgg
      841 ttaaagctgc aactgagacg aatttgccgc ttctgtttct accagttcat agatcccata
      901 ttgactatct gctgctcact ttcattctct tctgccataa catcaaagca ccatacattg
      961 cttcaggcaa taatctcaac atcccaatct tcagtacctt gatccataag cttgggggct
     1021 tcttcatacg acgaaggctc gatgaaacac cagatggacg gaaagatgtt ctctatagag
     1081 ctttgctcca tgggcatata gttgaattac ttcgacagca gcaattcttg gagatcttcc
     1141 tggaaggcac acgttctagg agtggaaaaa cctcttgtgc tcgggcagga cttttgtcag
     1201 ttgtggtaga tactctgtct accaatgtca tcccagacat cttgataata cctgttggaa
     1261 tctcctatga tcgcattatc gaaggtcact acaatggtga acaactgggc aaacctaaga
     1321 agaatgagag cctgtggagt gtagcaagag gtgttattag aatgttacga aaaaactatg
     1381 gttgtgtccg agtggatttt gcacagccat tttccttaaa ggaatattta gaaagccaaa
     1441 gtcagaaacc ggtgtctgct ctactttccc tggagcaagc gttgttacca gctatacttc
     1501 cttcaagacc cagtgatgct gctgatgaag gtagagacac gtccattaat gagtccagaa
     1561 atgcaacaga tgaatcccta cgaaggaggt tgattgcaaa tctggctgag catattctat
     1621 tcactgctag caagtcctgt gccattatgt ccacacacat tgtggcttgc ctgctcctct
     1681 acagacacag gcagggaatt gatctctcca cattggtcga agacttcttt gtgatgaaag
     1741 aggaagtcct ggctcgtgat tttgacctgg ggttctcagg aaattcagaa gatgtagtaa
     1801 tgcatgccat acagctgctg ggaaattgtg tcacaatcac ccacactagc aggaacgatg
     1861 agttttttat cacccccagc acaactgtcc catcagtctt cgaactcaac ttctacagca
     1921 atggggtact tcatgtcttt atcatggagg ccatcatagc ttgcagcctt tatgcagttc
     1981 tgaacaagag gggactgggg ggtcccacta gcaccccacc taacctgatc agccaggagc
     2041 agctggtgcg gaaggcggcc agcctgtgct accttctctc caatgaaggc accatctcac
     2101 tgccttgcca gacattttac caagtctgcc atgaaacagt aggaaagttt atccagtatg
     2161 gcattcttac agtggcagag cacgatgacc aggaagatat cagtcctagt cttgctgagc
     2221 agcagtggga caagaagctt ccagaacctt tgtcttggag aagtgatgaa gaagatgaag
     2281 acagtgactt tggggaggaa cagcgagatt gctacctgaa ggtgagccaa tccaaggagc
     2341 accagcagtt tatcaccttc ttacagagac tccttgggcc tttgctggag gcctacagct
     2401 ctgctgccat ctttgttcac aacttcagtg gtcctgttcc agaacctgag tatctgcaaa
     2461 agttgcacaa atacctaata accagaacag aaagaaatgt tgcagtatat gctgagagtg
     2521 ccacatattg tcttgtgaag aatgctgtga aaatgtttaa ggatattggg gttttcaagg
     2581 agaccaaaca aaagagagtg tctgttttag aactgagcag cacttttcta cctcaatgca
     2641 accgacaaaa acttctagaa tatattctga gttttgtggt gctgtaggta acgtgtggca
     2701 ctgctggcaa atgaaggtca tgagatgagt tccttgtagg taccagcttc tggctcaaga
     2761 gttgaaggtg ccatcgcagg gtcaggcctg ccctgtcccg aagtgatctc ctggaagaca
     2821 agtgccttct ccctccatgg atctgtgatc ttcccagctc tgcatcaaca cagcagcctg
     2881 cagataacac ttggggggac ctcagcctct attcgcaact cataatccgt agactacaag
     2941 atgaaatctc aataaattat ttttgagttt attaaagatt gacattttaa gtacaacttt
     3001 taaggactaa ttactgtgat ggacacagaa atgtagctgt gttctggaac tgaatcttac
     3061 atggtatact tagtgctgct gggtaatttg ttggtatatt atctggttag tggttaatgc
     3121 ttcctttaaa aataattgag tcatccattc actctttttc agttttatct gtcaatagta
     3181 gctacatttt taatgggagc accttttatc ccaaagtgct ttataaattg agtggactga
     3241 tatatatcac acccaggtat cactgtgctg tcctttgctg tcagatttag aaatgttttt
     3301 aagagctatg tgaaaacaga caatattagt ttaggtcggg aactgagata ttgtaatcaa
     3361 atagttaaca tcaggaagtt aatttggctg gcaaaattct agggaaactt ggccagaaaa
     3421 ctggtgttga aggcttttgc tcatataaac aagtgccatt gagtttcaaa tgaccagcaa
     3481 atatatttag aacccttcct gttttatgtc tgtacctcgt ccacccctca ggtaatacct
     3541 gcctctcaca ggtacagctg tttcttggaa atcctccaac caaatagcag ttttcctaac
     3601 ttgattagct tgagctgaca gactgttaga atacagttct ctggccacag ctgatgaggg
     3661 ctttctgtac tgcacacaga ttgtgtactg caccccagtc caggtgactg gtacccactc
     3721 gagttgtgcc gtgcacaacc tgtccagtat atgcatgtgg tggccctact gactggtaat
     3781 ggttagaggc atttatggat ttttagcttt gaggaaaaac catgactttt aacaaatttt
     3841 tatgggttat atgcctaaac ccttatgcca catagtggta aataattatg aaaaatggtc
     3901 tgttcataat tggtaggtgc cttttgtgag cagggagcat aattattggt ttattatggt
     3961 aattatggtg attttttaaa tatcatgtaa tgttaaaacg ttttctaaca gtttactgtt
     4021 gcttatctcc aagatattat ggaattaaga atttttccag atgagtgtta catagattct
     4081 ttgaatttag tataaaagta ctgagaatta agtttgtact tccataagct tggattttaa
     4141 acactgatag tatctcatga gtaatgtgtg ttttgggaga gggagggatg ctgattgata
     4201 tttcacattg tatgaaatac catgtttgaa actcatagca ataatgctat gctgttgtga
     4261 tccctctcaa gttctgcatt taaaatatat tttttcttta taggaattga tgtataccat
     4321 gaagtcattg tcagttgtag tagctctgat gttgaatgag atatcatgtt ttagcattcc
     4381 attttactga ctagggtaga agaacacttt tcttggctac atttggagga tacccaggga
     4441 gtcttgggtg ttccttatct ggggaagcaa acatttcact agtctctttt tttcatcctt
     4501 taaattgtaa attaaggatt actcaagctc accattattc aagattggga ctcgcttccc
     4561 agtcgacact ctgccctgcc tgtcattgct gcaaagagct gctgctttgc caacctaagc
     4621 aaagaaaata cggcttctct tgcattattt tcccttttgg ttggtttgtt ttctagaagt
     4681 acgttcagat gctttgggga atgcaatgta tgatttgcta gctctctcac cacttaactc
     4741 actgtgagga taaatatgca tgctttttgt aattaactgg tgctttgaaa atctttttta
     4801 agggagaaaa atctcaacca aagttatgct catccagaca agctgacctt tgagttaatt
     4861 tcagcacaac tcattcttca gtgcctcatg actgaaaaca aaaaacaaaa aaacgaaagc
     4921 atcttcacaa tgaagcttcc agatagcacc gttttgctaa aagatacatt ctcattgttt
     4981 tccaacagtg atggcttcca cataaggtta aacaaactag gtgcttgtaa ataatttatt
     5041 acagtttact ctatcgcatt tctgtaacat gaaatgcatg cccttcttca ggggaagact
     5101 gtggtcaagt taaaaaaaaa aaacaatatt aaacaacatg aaactgcagt ctgtttttga
     5161 aaatgagaat gtcctaagtg attcagaaga gaggagggaa gttgtgcact ctgaaaatgc
     5221 atgaaaaaca aaggcaaaaa ctagtgggaa atgtgtagaa ctgttaactg agatggcttc
     5281 gagtcttcct tctggaatct gttaaatttc acaaagtcat gagggtaaat ggagaaaata
     5341 tttctgggat tacaatgaat gtaagcccaa attgtggaat tgccagtaac ctggatgggg
     5401 aaaagcattt cccatagcac tccatgtaat atgagtgctc tgtgagatgt tcatcagtgt
     5461 tttatagaaa tggtgttgct gggaaaccaa gtttgcacct ggaaacttac aatgcacttt
     5521 agcgcagtaa gggcttggca tccggtagtg aaaaactgtc taacccagca ttgcccaaac
     5581 tattttgaca ccaggacctt tttctccttt gggatactta tgaacctctc actaatgtcc
     5641 tgtggagaac attttgggaa acactatgtt agatagttct ttaaggagac aaaacggtaa
     5701 tgaacagata gcactggggc agaatatgca tgcattttgt aacgtccagt gtggcgttga
     5761 atagatgtgt atttcctccc ctgcagaaaa taagcacaga aaattataat gtaggtgatc
     5821 ggagctcttt cctttgatag agagaacagc cccaatgatc ctggcttttt cactgaacgt
     5881 atcagaatac atggatgaat tggggtaaat aaggttttaa ttcagatcta gaagaaagta
     5941 ttgtacgttt gaatgcagat ttttatccac agatagttgt agtgtttaga catgacagga
     6001 cctatcgttg aggtttctaa gacttactat gggctgtaaa cctgtttttt aaaactattt
     6061 tagaaacctg agacttgccg tctggcattt tagtttaata caaactaatg attgcatttg
     6121 aaagagattc ttgaccttat ttctaaacgt ctagagctct gaaatgtctt gatggaaggt
     6181 attaaactat ttgcctgttg tacaaagaaa tgttaagact cgtgaaaaga attactataa
     6241 ggtactgtga aataactgcg attttgtgag caaaacatac ttggaaatgc tgattgattt
     6301 ttatgcttgt tagtgtattg caagaaacac agaaaatgta gttttgtttt aataaaccaa
     6361 aaattgaaca ta
//
